MD13G1203100.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012768
Physical Location & Seq
Reverse (-)
18259785 .. 18262807
3023 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1203100.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGGCGGTGCAGAATCAGAACCAGGGAGAGAAATTGAACTTGGGACCTGAGTCAGCAGGAAGTCGCAAACCTGCGCTTCCTAACTTTCTTTTATCAGTGAAGCTCAAATATGTCAAGCTAGGGTACCACTACTTAATCTCCAACGCCCTCTACCTCCTACTAGTCCCTCTCCTTGCCGCGGCCTCCGCCCACCTCTCTACGCTCACAGCCGATGACTTCTTCCACCTGTGGCAGCAGCTCAAGTTCAACTTCGTCTCGGTCACTTTGTGCTCCGGCCTCTTGGTTTTCCTAACCACCGTATACTTCACCACCCGCCCTAGAAAAATATACTTGGTGAACTTCGCATGCTACAAGCCCGAGCCGGCCCGTACGTGCACACGTGAGATGTTCATGGAGAGGTCGAAGCTGACGGGCAGCTTCTACGACGAGAACTTGGCTTTTCAGCAGAAGATTCTCGAGAGGTCTGGCCTCGGGCAGAATACGTACATGCCTGAGGCCGTGATGCGGGTCCCACCGAACCCGTGCATGGTGGAGGCGAGGAAGGAGGCGGAGATGGTGATGTTTGGAGCGATCGACGAGTTGCTGGAGAAGACTGGGGTGAAGGCTAAGGATATTGGAATTCTGGTGGTGAACTGCAGCTTGTTCAATCCGACGCCGTCTTTGTCGTCCATGGTTGTGAATCACTACAAGCTTAGGGGGAACGTTGTGAGCTACAACCTTGGTGGGATGGGTTGCAGTGCTGGTCTCATTTCTATTGACCTTGCAAAGCAGCTTCTCCAGGTGCACCCCAACTCTTATGCCCTAGTGGTAAGCATGGAGAACATCACTCTCAATTGGTACTTTGGCAATGACCGCTCTATGCTTCTCTCCAACTGCCTCTTTCGAATGGGCGGCGCCGCCATCCTCCTCTCCAACCGCTCCTCTGACCACCGCCGCTCAAAGTACCAGCTCATGCACGCGGTCCGCACGCACAAGGGTGCAGACGACAGGGCATATAATTGCGTGTTCCAAAAGGAGGACGACACGAAAAGAGTCGGCGTTGCTCTCTCGAAAGACTTGATGGCTGTGGCTGGTGAAGCCCTAAAAACCAACATCACCACACTTGGTCCAATAGTCCTCCCCATGTCAGAGCAACTCCTGTTTTTTGCTACTTTGGTGGCAAGAAAGGTGTTCAAGATGAAGAACATCAAACCCTACATCCCGGATTTCAAGCTGGCGTTTGAGCACTTCTGCATTCACGCCGGAGGGAGAGCGGTGCTGGATGAGATCGAGAAGAACCTTGAGCTCAGCGACTGGCACATGGAGCCATCGAGGATGACTCTCTATAGGTTTGGGAACACTTCAAGCAGCTCATTGTGGTACGAGCTGGCTTACTCTGAGGCCAAGGGAAGAGTCCGAAAGGGAGACAGGATGTGGCAAATTGCGTTCGGGTCGGGGTTCAAGTGCAACAGCGCGGTGTGGAAGGCGTTAAGGACTGTTAAACCAGCCAAGGAGAAGAACCCTTGGATTGATGAGATTCATGAGTTTCCTGTAGAGGTGCCTAAAGTGTCAGCCGTTGCTTCTTAA

Protein Analysis

522

Amino Acids

58.58

Weight (kDa)

9.26

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 101 - 390 1.3e-142 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 408 - 488 7.9e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 410 - 486 3.2e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012768)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04220 AT5G43760
fragaria_vesca FvH4_4g01980 FvH4_7g17470
malus_domestica MD13G1203100.v1.1
prunus_persica Prupe.1G025600_v2.0.a1
pyrus_communis pycom07g15420 pycom13g17640 pycom16g17200
rosa_chinensis RchiOBHm_Chr4g0388941
rosa_laevigata RLG00000010005
rosa_multiflora Rmu_sc0005486.1_g000002
rosa_roxburghii Rroxscaffold_5G00335260
rosa_rugosa Rorug03G0321500
rosa_samantha Rh4AG025900 Rh4BG019200 Rh4DG019700
rosa_wichuraiana Rw4G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 79
Acc65I GGTACC 1 cut(s) 123
AccB1I GGYRCC 3 cut(s) 123, 893, 1537
AccBSI CCGCTC 4 cut(s) 855, 918, 936, 1253
AccI GTMKAC 1 cut(s) 300
AccII CGCG 3 cut(s) 179, 959, 1454
AclI AACGTT 1 cut(s) 702
AcsI RAATTY 1 cut(s) 618
AcvI CACGTG 1 cut(s) 380
AcyI GRCGYC 2 cut(s) 653, 894
AdeI CACNNNGTG 1 cut(s) 267
AfaI GTAC 6 cut(s) 125, 370, 485, 839, 944, 1361
AfiI CCNNNNNNNGG 4 cut(s) 178, 504, 526, 1015
AflIII ACRYGT 1 cut(s) 377
AgsI TTSAA 7 cut(s) 37, 247, 646, 1174, 1210, 1344, 1441
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 2 cut(s) 21, 777
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AleI CACNNNNGTG 1 cut(s) 975
Alw21I GWGCWC 5 cut(s) 272, 377, 786, 1227, 1287
Alw26I GTCTC 3 cut(s) 259, 749, 1398
Alw44I GTGCAC 2 cut(s) 373, 782
AlwNI CAGNNNCTG 1 cut(s) 1293
Ama87I CYCGRG 3 cut(s) 356, 455, 470
AoxI GGCC 6 cut(s) 180, 274, 363, 466, 495, 1380
ApaLI GTGCAC 2 cut(s) 373, 782
ApeKI GCWGC 6 cut(s) 232, 235, 414, 636, 769, 1347
ApoI RAATTY 1 cut(s) 618
ArsI GACNNNNNNTTYG 2 cut(s) 643, 675
Asp718I GGTACC 1 cut(s) 123
AspLEI GCGC 3 cut(s) 76, 896, 1454
AspS9I GGNCC 5 cut(s) 44, 364, 508, 961, 1106
AsuC2I CCSGG 1 cut(s) 1202
AsuHPI GGTGA 7 cut(s) 298, 346, 568, 610, 640, 1085, 1087
AsuII TTCGAA 1 cut(s) 883
AvaI CYCGRG 3 cut(s) 356, 455, 470
AvaII GGWCC 4 cut(s) 44, 508, 961, 1106
AxyI CCTNAGG 1 cut(s) 492
BaeGI GKGCMC 2 cut(s) 377, 786
BanI GGYRCC 3 cut(s) 123, 893, 1537
BanII GRGCYC 1 cut(s) 1287
BbrPI CACGTG 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 596
Bbv12I GWGCWC 5 cut(s) 272, 377, 786, 1227, 1287
BbvI GCAGC 6 cut(s) 244, 247, 426, 648, 781, 1359
BccI CCATC 5 cut(s) 547, 721, 908, 1054, 1315
BceAI ACGGC 3 cut(s) 482, 640, 1538
BciT130I CCWGG 2 cut(s) 23, 779
BcnI CCSGG 1 cut(s) 1202
BcoDI GTCTC 3 cut(s) 259, 749, 1398
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 4 cut(s) 119, 161, 318, 803
BfmI CTRYAG 3 cut(s) 634, 1324, 1530
BfoI RGCGCY 1 cut(s) 897
BfuAI ACCTGC 1 cut(s) 79
BlpI GCTNAGC 1 cut(s) 1286
Bme1390I CCNGG 3 cut(s) 23, 779, 1202
Bme18I GGWCC 4 cut(s) 44, 508, 961, 1106
BmeT110I CYCGRG 3 cut(s) 356, 455, 470
BmgT120I GGNCC 5 cut(s) 44, 364, 508, 961, 1106
BmiI GGNNCC 7 cut(s) 45, 125, 509, 510, 895, 1305, 1539
BmrFI CCNGG 3 cut(s) 23, 779, 1202
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 603
BoxI GACNNNNGTC 1 cut(s) 49
BpiI GAAGAC 1 cut(s) 596
BplI GAGNNNNNCTC 2 cut(s) 1303, 1335
BpmI CTGGAG 2 cut(s) 605, 761
Bpu10I CCTNAGC 2 cut(s) 606, 692
Bpu1102I GCTNAGC 1 cut(s) 1286
Bpu14I TTCGAA 1 cut(s) 883
BpuEI CTTGAG 2 cut(s) 224, 1301
BpuMI CCSGG 1 cut(s) 1202
BsaAI YACGTR 3 cut(s) 372, 380, 483
BsaHI GRCGYC 2 cut(s) 653, 894
BsaI GGTCTC 1 cut(s) 749
BsaJI CCNNGG 8 cut(s) 22, 177, 469, 669, 718, 1383, 1488, 1502
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 4 cut(s) 178, 504, 526, 1015
Bse118I RCCGGY 1 cut(s) 361
Bse1I ACTGG 2 cut(s) 598, 1298
Bse21I CCTNAGG 1 cut(s) 492
Bse3DI GCAATG 1 cut(s) 853
BseBI CCWGG 2 cut(s) 23, 779
BseDI CCNNGG 8 cut(s) 22, 177, 469, 669, 718, 1383, 1488, 1502
BseGI GGATG 6 cut(s) 732, 900, 1197, 1267, 1320, 1416
BseLI CCNNNNNNNGG 4 cut(s) 178, 504, 526, 1015
BseMI GCAATG 1 cut(s) 853
BseMII CTCAG 4 cut(s) 39, 483, 1300, 1368
BseNI ACTGG 2 cut(s) 598, 1298
BseRI GAGGAG 2 cut(s) 896, 910
BseSI GKGCMC 2 cut(s) 377, 786
BseXI GCAGC 6 cut(s) 244, 247, 426, 648, 781, 1359
BsgI GTGCAG 2 cut(s) 29, 999
Bsh1236I CGCG 3 cut(s) 179, 959, 1454
Bsh1285I CGRYCG 1 cut(s) 573
BshFI GGCC 6 cut(s) 182, 276, 365, 468, 497, 1382
BshNI GGYRCC 3 cut(s) 123, 893, 1537
BsiEI CGRYCG 1 cut(s) 573
BsiHKAI GWGCWC 5 cut(s) 272, 377, 786, 1227, 1287
BsiHKCI CYCGRG 3 cut(s) 356, 455, 470
BsiSI CCGG 4 cut(s) 273, 362, 1202, 1242
BsiWI CGTACG 1 cut(s) 368
BslFI GGGAC 3 cut(s) 57, 149, 494
BslI CCNNNNNNNGG 4 cut(s) 178, 504, 526, 1015
BsmAI GTCTC 3 cut(s) 259, 749, 1398
BsmBI CGTCTC 1 cut(s) 259
BsmFI GGGAC 3 cut(s) 57, 149, 494
BsmI GAATGC 1 cut(s) 1233
BsnI GGCC 6 cut(s) 182, 276, 365, 468, 497, 1382
Bso31I GGTCTC 1 cut(s) 749
BsoBI CYCGRG 3 cut(s) 356, 455, 470
Bsp119I TTCGAA 1 cut(s) 883
Bsp1286I GDGCHC 5 cut(s) 272, 377, 786, 1227, 1287
Bsp143I GATC 2 cut(s) 570, 1266
Bsp1720I GCTNAGC 1 cut(s) 1286
Bsp19I CCATGG 1 cut(s) 669
BspANI GGCC 6 cut(s) 182, 276, 365, 468, 497, 1382
BspCNI CTCAG 4 cut(s) 40, 484, 1299, 1369
BspFNI CGCG 3 cut(s) 179, 959, 1454
BspHI TCATGA 1 cut(s) 1519
BspLI GGNNCC 7 cut(s) 45, 125, 509, 510, 895, 1305, 1539
BspMAI CTGCAG 1 cut(s) 638
BspMI ACCTGC 1 cut(s) 79
BspT104I TTCGAA 1 cut(s) 883
BspT107I GGYRCC 3 cut(s) 123, 893, 1537
BspTNI GGTCTC 1 cut(s) 749
BsrBI CCGCTC 4 cut(s) 855, 918, 936, 1253
BsrDI GCAATG 1 cut(s) 853
BsrFI RCCGGY 1 cut(s) 361
BsrI ACTGG 2 cut(s) 598, 1298
BssAI RCCGGY 1 cut(s) 361
BssECI CCNNGG 8 cut(s) 22, 177, 469, 669, 718, 1383, 1488, 1502
BssMI GATC 2 cut(s) 570, 1266
BssNAI GTATAC 1 cut(s) 301
BssNI GRCGYC 2 cut(s) 653, 894
BssT1I CCWWGG 5 cut(s) 669, 718, 1383, 1488, 1502
Bst1107I GTATAC 1 cut(s) 301
Bst2UI CCWGG 2 cut(s) 23, 779
Bst4CI ACNGT 2 cut(s) 298, 1477
Bst6I CTCTTC 1 cut(s) 1384
BstACI GRCGYC 2 cut(s) 653, 894
BstBAI YACGTR 3 cut(s) 372, 380, 483
BstBI TTCGAA 1 cut(s) 883
BstC8I GCNNGC 6 cut(s) 346, 363, 957, 968, 1215, 1368
BstDEI CTNAG 6 cut(s) 48, 492, 606, 692, 1286, 1377
BstDSI CCRYGG 2 cut(s) 177, 669
BstF5I GGATG 6 cut(s) 732, 900, 1197, 1267, 1320, 1416
BstFNI CGCG 3 cut(s) 179, 959, 1454
BstH2I RGCGCY 1 cut(s) 897
BstHHI GCGC 3 cut(s) 76, 896, 1454
BstKTI GATC 2 cut(s) 573, 1269
BstMAI GTCTC 3 cut(s) 259, 749, 1398
BstMBI GATC 2 cut(s) 570, 1266
BstMCI CGRYCG 1 cut(s) 573
BstMWI GCNNNNNNNGC 4 cut(s) 185, 852, 1076, 1303
BstNI CCWGG 2 cut(s) 23, 779
BstNSI RCATGY 2 cut(s) 348, 490
BstPAI GACNNNNGTC 1 cut(s) 49
BstSCI CCNGG 3 cut(s) 21, 777, 1200
BstSFI CTRYAG 3 cut(s) 634, 1324, 1530
BstSLI GKGCMC 2 cut(s) 377, 786
BstSNI TACGTA 1 cut(s) 483
BstUI CGCG 3 cut(s) 179, 959, 1454
BstV1I GCAGC 6 cut(s) 244, 247, 426, 648, 781, 1359
BstV2I GAAGAC 1 cut(s) 596
BstZ17I GTATAC 1 cut(s) 301
Bsu36I CCTNAGG 1 cut(s) 492
BsuRI GGCC 6 cut(s) 182, 276, 365, 468, 497, 1382
BtgI CCRYGG 2 cut(s) 177, 669
BtsCI GGATG 6 cut(s) 732, 900, 1197, 1267, 1320, 1416
BtsI GCAGTG 1 cut(s) 742
BtsIMutI CAGTG 2 cut(s) 102, 742
BveI ACCTGC 1 cut(s) 79
Cac8I GCNNGC 6 cut(s) 346, 363, 957, 968, 1215, 1368
CaiI CAGNNNCTG 1 cut(s) 1293
CciI TCATGA 1 cut(s) 1519
CfoI GCGC 3 cut(s) 76, 896, 1454
Cfr10I RCCGGY 1 cut(s) 361
Cfr13I GGNCC 5 cut(s) 44, 364, 508, 961, 1106
Cfr42I CCGCGG 1 cut(s) 180
CpoI CGGWCCG 1 cut(s) 961
CseI GACGC 1 cut(s) 661
Csp6I GTAC 6 cut(s) 124, 369, 484, 838, 943, 1360
CspCI CAANNNNNGTGG 2 cut(s) 703, 738
CspI CGGWCCG 1 cut(s) 961
CviQI GTAC 6 cut(s) 124, 369, 484, 838, 943, 1360
DdeI CTNAG 6 cut(s) 48, 492, 606, 692, 1286, 1377
DinI GGCGCC 1 cut(s) 895
DpnI GATC 2 cut(s) 572, 1268
DpnII GATC 2 cut(s) 570, 1266
DraIII CACNNNGTG 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 1384
EarI CTCTTC 1 cut(s) 1384
EciI GGCGGA 2 cut(s) 175, 563
Ecl136II GAGCTC 1 cut(s) 1285
Eco105I TACGTA 1 cut(s) 483
Eco130I CCWWGG 5 cut(s) 669, 718, 1383, 1488, 1502
Eco24I GRGCYC 1 cut(s) 1287
Eco31I GGTCTC 1 cut(s) 749
Eco47I GGWCC 4 cut(s) 44, 508, 961, 1106
Eco53kI GAGCTC 1 cut(s) 1285
Eco72I CACGTG 1 cut(s) 380
Eco81I CCTNAGG 1 cut(s) 492
Eco88I CYCGRG 3 cut(s) 356, 455, 470
EcoICRI GAGCTC 1 cut(s) 1285
EcoO109I RGGNCCY 2 cut(s) 44, 508
EcoRI GAATTC 1 cut(s) 618
EcoRII CCWGG 2 cut(s) 21, 777
EcoT14I CCWWGG 5 cut(s) 669, 718, 1383, 1488, 1502
EcoT38I GRGCYC 1 cut(s) 1287
EgeI GGCGCC 1 cut(s) 895
EheI GGCGCC 1 cut(s) 895
ErhI CCWWGG 5 cut(s) 669, 718, 1383, 1488, 1502
Esp3I CGTCTC 1 cut(s) 259
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 3 cut(s) 57, 149, 494
FauI CCCGC 2 cut(s) 320, 498
FblI GTMKAC 1 cut(s) 300
FokI GGATG 6 cut(s) 739, 887, 1184, 1274, 1327, 1423
FriOI GRGCYC 1 cut(s) 1287
FspBI CTAG 4 cut(s) 119, 161, 318, 803
GlaI GCGC 3 cut(s) 75, 895, 1453
GsuI CTGGAG 2 cut(s) 605, 761
HaeII RGCGCY 1 cut(s) 897
HaeIII GGCC 6 cut(s) 182, 276, 365, 468, 497, 1382
HapII CCGG 4 cut(s) 273, 362, 1202, 1242
HgaI GACGC 1 cut(s) 661
HhaI GCGC 3 cut(s) 76, 896, 1454
Hin1I GRCGYC 2 cut(s) 653, 894
Hin6I GCGC 3 cut(s) 74, 894, 1452
HinP1I GCGC 3 cut(s) 74, 894, 1452
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 8 cut(s) 13, 50, 451, 679, 1032, 1318, 1392, 1516
HpaII CCGG 4 cut(s) 273, 362, 1202, 1242
HphI GGTGA 7 cut(s) 298, 346, 568, 610, 640, 1085, 1087
Hpy166II GTNNAC 5 cut(s) 301, 337, 375, 631, 784
Hpy188I TCNGA 6 cut(s) 18, 651, 925, 1129, 1378, 1397
Hpy188III TCNNGA 6 cut(s) 455, 457, 1048, 1174, 1270, 1520
Hpy8I GTNNAC 5 cut(s) 301, 337, 375, 631, 784
Hpy99I CGWCG 3 cut(s) 428, 578, 655
HpyAV CCTTC 3 cut(s) 535, 595, 1456
HpyCH4III ACNGT 2 cut(s) 298, 1477
HpyCH4IV ACGT 4 cut(s) 371, 379, 482, 702
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 852, 1076, 1303
HpyF3I CTNAG 6 cut(s) 48, 492, 606, 692, 1286, 1377
HpySE526I ACGT 4 cut(s) 371, 379, 482, 702
Hsp92I GRCGYC 2 cut(s) 653, 894
HspAI GCGC 3 cut(s) 74, 894, 1452
KasI GGCGCC 1 cut(s) 893
KflI GGGWCCC 1 cut(s) 508
KpnI GGTACC 1 cut(s) 127
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
KspI CCGCGG 1 cut(s) 180
Kzo9I GATC 2 cut(s) 570, 1266
LmnI GCTCC 4 cut(s) 275, 566, 923, 1303
Lsp1109I GCAGC 6 cut(s) 244, 247, 426, 648, 781, 1359
LweI GCATC 1 cut(s) 492
MaeI CTAG 4 cut(s) 119, 161, 318, 803
MaeII ACGT 4 cut(s) 371, 379, 482, 702
MaeIII GTNAC 1 cut(s) 259
MalI GATC 2 cut(s) 572, 1268
MbiI CCGCTC 4 cut(s) 855, 918, 936, 1253
MboI GATC 2 cut(s) 570, 1266
MboII GAAGA 7 cut(s) 211, 460, 601, 1192, 1285, 1401, 1507
MfeI CAATTG 1 cut(s) 832
MhlI GDGCHC 5 cut(s) 272, 377, 786, 1227, 1287
MluCI AATT 5 cut(s) 32, 618, 832, 997, 1419
Mly113I GGCGCC 1 cut(s) 894
MlyI GAGTC 4 cut(s) 59, 1041, 1312, 1401
MmeI TCCRAC 4 cut(s) 165, 674, 894, 936
MroNI GCCGGC 1 cut(s) 361
MseI TTAA 4 cut(s) 134, 1469, 1479, 1564
MslI CAYNNNNRTG 2 cut(s) 674, 975
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 4 cut(s) 273, 362, 1202, 1242
MspR9I CCNGG 3 cut(s) 23, 779, 1202
MunI CAATTG 1 cut(s) 832
Mva1269I GAATGC 1 cut(s) 1233
MvaI CCWGG 2 cut(s) 23, 779
MvnI CGCG 3 cut(s) 179, 959, 1454
MwoI GCNNNNNNNGC 4 cut(s) 185, 852, 1076, 1303
NaeI GCCGGC 1 cut(s) 363
NarI GGCGCC 1 cut(s) 894
NciI CCSGG 1 cut(s) 1202
NcoI CCATGG 1 cut(s) 669
NdeII GATC 2 cut(s) 570, 1266
NgoMIV GCCGGC 1 cut(s) 361
NlaIV GGNNCC 7 cut(s) 45, 125, 509, 510, 895, 1305, 1539
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 2 cut(s) 348, 490
NspV TTCGAA 1 cut(s) 883
OliI CACNNNNGTG 1 cut(s) 975
PaeI GCATGC 1 cut(s) 348
PaeR7I CTCGAG 1 cut(s) 455
PagI TCATGA 1 cut(s) 1519
PctI GAATGC 1 cut(s) 1233
PdiI GCCGGC 1 cut(s) 363
PfeI GAWTC 4 cut(s) 13, 451, 679, 1516
Pfl23II CGTACG 1 cut(s) 368
PflFI GACNNNGTC 1 cut(s) 655
PfoI TCCNGGA 1 cut(s) 1200
Ple19I CGATCG 1 cut(s) 573
PleI GAGTC 4 cut(s) 58, 1040, 1312, 1400
PluTI GGCGCC 1 cut(s) 897
PmaCI CACGTG 1 cut(s) 380
PmlI CACGTG 1 cut(s) 380
PpsI GAGTC 4 cut(s) 58, 1040, 1312, 1400
Ppu21I YACGTR 3 cut(s) 372, 380, 483
PpuMI RGGWCCY 2 cut(s) 44, 508
PshAI GACNNNNGTC 1 cut(s) 49
Psp124BI GAGCTC 1 cut(s) 1287
Psp1406I AACGTT 1 cut(s) 702
Psp5II RGGWCCY 2 cut(s) 44, 508
Psp6I CCWGG 2 cut(s) 21, 777
PspCI CACGTG 1 cut(s) 380
PspGI CCWGG 2 cut(s) 21, 777
PspLI CGTACG 1 cut(s) 368
PspN4I GGNNCC 7 cut(s) 45, 125, 509, 510, 895, 1305, 1539
PspPI GGNCC 5 cut(s) 44, 364, 508, 961, 1106
PspPPI RGGWCCY 2 cut(s) 44, 508
PstI CTGCAG 1 cut(s) 638
PstNI CAGNNNCTG 1 cut(s) 1293
PsyI GACNNNGTC 1 cut(s) 655
PvuI CGATCG 1 cut(s) 573
RsaI GTAC 6 cut(s) 125, 370, 485, 839, 944, 1361
RsaNI GTAC 6 cut(s) 124, 369, 484, 838, 943, 1360
RseI CAYNNNNRTG 2 cut(s) 674, 975
Rsr2I CGGWCCG 1 cut(s) 961
RsrII CGGWCCG 1 cut(s) 961
SacI GAGCTC 1 cut(s) 1287
SacII CCGCGG 1 cut(s) 180
SaqAI TTAA 4 cut(s) 134, 1469, 1479, 1564
Sau3AI GATC 2 cut(s) 570, 1266
Sau96I GGNCC 5 cut(s) 44, 364, 508, 961, 1106
SchI GAGTC 4 cut(s) 59, 1041, 1312, 1401
ScrFI CCNGG 3 cut(s) 23, 779, 1202
SduI GDGCHC 5 cut(s) 272, 377, 786, 1227, 1287
SfaNI GCATC 1 cut(s) 492
SfcI CTRYAG 3 cut(s) 634, 1324, 1530
SfoI GGCGCC 1 cut(s) 895
Sfr274I CTCGAG 1 cut(s) 455
Sfr303I CCGCGG 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 883
SgrBI CCGCGG 1 cut(s) 180
SinI GGWCC 4 cut(s) 44, 508, 961, 1106
SlaI CTCGAG 1 cut(s) 455
SmiMI CAYNNNNRTG 2 cut(s) 674, 975
SmlI CTYRAG 3 cut(s) 239, 455, 1280
SmoI CTYRAG 3 cut(s) 239, 455, 1280
SnaBI TACGTA 1 cut(s) 483
SpeI ACTAGT 1 cut(s) 160
SphI GCATGC 1 cut(s) 348
Sse9I AATT 5 cut(s) 32, 618, 832, 997, 1419
SspDI GGCGCC 1 cut(s) 893
SspMI CTAG 4 cut(s) 119, 161, 318, 803
SstI GAGCTC 1 cut(s) 1287
StyD4I CCNGG 3 cut(s) 21, 777, 1200
StyI CCWWGG 5 cut(s) 669, 718, 1383, 1488, 1502
TaaI ACNGT 2 cut(s) 298, 1477
TaiI ACGT 4 cut(s) 374, 382, 485, 705
TaqI TCGA 7 cut(s) 401, 456, 573, 883, 1049, 1269, 1310
TaqII GACCGA 1 cut(s) 247
TasI AATT 5 cut(s) 32, 618, 832, 997, 1419
TauI GCSGC 5 cut(s) 179, 182, 894, 899, 936
TfiI GAWTC 4 cut(s) 13, 451, 679, 1516
Tru1I TTAA 4 cut(s) 134, 1469, 1479, 1564
Tru9I TTAA 4 cut(s) 134, 1469, 1479, 1564
TscAI CASTG 2 cut(s) 102, 742
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 6 cut(s) 232, 235, 414, 636, 769, 1347
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 3 cut(s) 379, 1193, 1508
TspRI CASTG 2 cut(s) 102, 742
Tth111I GACNNNGTC 1 cut(s) 655
VneI GTGCAC 2 cut(s) 373, 782
VpaK11BI GGWCC 4 cut(s) 44, 508, 961, 1106
XapI RAATTY 1 cut(s) 618
XceI RCATGY 2 cut(s) 348, 490
XhoI CTCGAG 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 300
XspI CTAG 4 cut(s) 119, 161, 318, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.