pycom07g15420

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012768
Physical Location & Seq
Forward (+)
17729680 .. 17730372
693 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g15420.1

Sequence Viewer

Length: 693 bp
ATGCTCCTCTGCAACTGTATTTTCCGAATGGGCGGCGCCGCGGTTCTTCTATCGAACAGGAACCGCGACCGCCCCCGGTCCAAGTACGAGCTCGTACACACTGTCCGGACCCACAAGGGCGCCGACGACAGGAACTACAAGTGTGTCTACCAGCGAGAAGACGACAAAGGGACCGTCGGCGTTTCGCTGGCGCGTGAGCTCATGGCCGTGGCGGGCGACGCATTGAAAACGAACATAACAACGCTGGGCCCACTCGTCCTGCCGTTTACCGAACAGGTGTTGTTTTTCATAACGCTGATTAGGAAGAAGGTTTTCAAGGCGAAGGTCAAACCGTACATTCCGGACTTCAAGCTCGCGTTCGAGCACTTCTGCATACACGCGGGTGGACGCGCCGTCCTGGACGAGCTGGAGAAGAATCTTCAACTCACTGAGTGGCACATGGAGCCGTCTCGGATGACGTTGCACCGGTTCGGCAACACGTCGAGCAGTTCACTGTGGTACGAGCTCTCGTACGCGGAGGCCAAGGGCCGGGTCGGGAGGGGTGACCGGGTTTGGCAGATTGCTTTCGGGTCGGGTTTTAAGTGTAACAGCGCGGTATGGCGGGCCCTCCGGCCGATTTCTGTCGGGGACGGGTTGGGGAACCCGTGGATGGACTCGATTGATAAGTACCCGGTCAAAGTCCAGATGGCTTAG

Protein Analysis

231

Amino Acids

26.06

Weight (kDa)

9.55

Isoelectric Point (pI)

21.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 1 - 104 3.6e-47 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 121 - 201 7.9e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 123 - 201 6.5e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012768)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04220 AT5G43760
fragaria_vesca FvH4_4g01980 FvH4_7g17470
malus_domestica MD13G1203100.v1.1
prunus_persica Prupe.1G025600_v2.0.a1
pyrus_communis pycom07g15420 pycom13g17640 pycom16g17200
rosa_chinensis RchiOBHm_Chr4g0388941
rosa_laevigata RLG00000010005
rosa_multiflora Rmu_sc0005486.1_g000002
rosa_roxburghii Rroxscaffold_5G00335260
rosa_rugosa Rorug03G0321500
rosa_samantha Rh4AG025900 Rh4BG019200 Rh4DG019700
rosa_wichuraiana Rw4G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 35, 119
AccI GTMKAC 1 cut(s) 147
AccII CGCG 8 cut(s) 41, 66, 193, 356, 380, 390, 515, 593
AccIII TCCGGA 2 cut(s) 105, 340
AcoI YGGCCR 2 cut(s) 204, 611
AcyI GRCGYC 2 cut(s) 36, 120
AdeI CACNNNGTG 1 cut(s) 432
AfaI GTAC 6 cut(s) 86, 96, 335, 500, 512, 668
AfiI CCNNNNNNNGG 3 cut(s) 129, 528, 649
AflIII ACRYGT 1 cut(s) 477
AgeI ACCGGT 1 cut(s) 465
AgsI TTSAA 4 cut(s) 226, 316, 349, 422
AhdI GACNNNNNGTC 1 cut(s) 392
AjiI CACGTC 1 cut(s) 480
AjnI CCWGG 1 cut(s) 396
AleI CACNNNNGTG 1 cut(s) 381
AluBI AGCT 5 cut(s) 91, 199, 352, 406, 505
AluI AGCT 5 cut(s) 91, 199, 352, 406, 505
Alw21I GWGCWC 4 cut(s) 93, 201, 366, 507
Alw26I GTCTC 1 cut(s) 453
Aor13HI TCCGGA 2 cut(s) 105, 340
AoxI GGCC 6 cut(s) 204, 247, 519, 526, 603, 611
ApaI GGGCCC 2 cut(s) 251, 607
ArsI GACNNNNNNTTYG 2 cut(s) 159, 191
AsiGI ACCGGT 1 cut(s) 465
Asp700I GAANNNNTTC 1 cut(s) 311
AspLEI GCGC 5 cut(s) 38, 122, 193, 392, 593
AspS9I GGNCC 8 cut(s) 78, 108, 171, 247, 248, 526, 603, 604
AsuC2I CCSGG 4 cut(s) 76, 530, 548, 671
AsuHPI GGTGA 1 cut(s) 554
AvaII GGWCC 3 cut(s) 78, 108, 171
BaeGI GKGCMC 2 cut(s) 251, 607
BanI GGYRCC 2 cut(s) 35, 119
BanII GRGCYC 5 cut(s) 93, 201, 251, 507, 607
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 4 cut(s) 93, 201, 366, 507
BccI CCATC 2 cut(s) 643, 679
BceAI ACGGC 4 cut(s) 191, 247, 377, 430
BciT130I CCWGG 1 cut(s) 398
BcnI CCSGG 4 cut(s) 76, 530, 548, 671
BcoDI GTCTC 1 cut(s) 453
BfoI RGCGCY 2 cut(s) 39, 123
BisI GCNGC 2 cut(s) 34, 39
BlsI GCNGC 2 cut(s) 35, 40
Bme1390I CCNGG 5 cut(s) 76, 398, 530, 548, 671
Bme18I GGWCC 3 cut(s) 78, 108, 171
BmeRI GACNNNNNGTC 1 cut(s) 392
BmgBI CACGTC 1 cut(s) 480
BmgT120I GGNCC 8 cut(s) 78, 108, 171, 247, 248, 526, 603, 604
BmiI GGNNCC 9 cut(s) 37, 62, 110, 121, 172, 249, 444, 605, 641
BmrFI CCNGG 5 cut(s) 76, 398, 530, 548, 671
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 428
BpuMI CCSGG 4 cut(s) 76, 530, 548, 671
BsaHI GRCGYC 2 cut(s) 36, 120
BsaJI CCNNGG 5 cut(s) 39, 74, 207, 522, 644
BsaWI WCCGGW 3 cut(s) 105, 340, 465
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 528, 649
Bse118I RCCGGY 1 cut(s) 465
BseAI TCCGGA 2 cut(s) 105, 340
BseBI CCWGG 1 cut(s) 398
BseDI CCNNGG 5 cut(s) 39, 74, 207, 522, 644
BseGI GGATG 2 cut(s) 459, 654
BseLI CCNNNNNNNGG 3 cut(s) 129, 528, 649
BseMII CTCAG 1 cut(s) 420
BseSI GKGCMC 2 cut(s) 251, 607
BseX3I CGGCCG 1 cut(s) 611
BseYI CCCAGC 1 cut(s) 244
Bsh1236I CGCG 8 cut(s) 41, 66, 193, 356, 380, 390, 515, 593
Bsh1285I CGRYCG 2 cut(s) 70, 614
BshFI GGCC 6 cut(s) 206, 249, 521, 528, 605, 613
BshNI GGYRCC 2 cut(s) 35, 119
BshTI ACCGGT 1 cut(s) 465
BsiEI CGRYCG 2 cut(s) 70, 614
BsiHKAI GWGCWC 4 cut(s) 93, 201, 366, 507
BsiSI CCGG 8 cut(s) 76, 106, 341, 466, 529, 547, 610, 671
BsiWI CGTACG 1 cut(s) 510
BslFI GGGAC 2 cut(s) 184, 641
BslI CCNNNNNNNGG 3 cut(s) 129, 528, 649
BsmAI GTCTC 1 cut(s) 453
BsmBI CGTCTC 1 cut(s) 453
BsmFI GGGAC 2 cut(s) 184, 641
BsnI GGCC 6 cut(s) 206, 249, 521, 528, 605, 613
Bsp120I GGGCCC 2 cut(s) 247, 603
Bsp1286I GDGCHC 6 cut(s) 93, 201, 251, 366, 507, 607
Bsp13I TCCGGA 2 cut(s) 105, 340
BspANI GGCC 6 cut(s) 206, 249, 521, 528, 605, 613
BspCNI CTCAG 1 cut(s) 421
BspEI TCCGGA 2 cut(s) 105, 340
BspFNI CGCG 8 cut(s) 41, 66, 193, 356, 380, 390, 515, 593
BspLI GGNNCC 9 cut(s) 37, 62, 110, 121, 172, 249, 444, 605, 641
BspT107I GGYRCC 2 cut(s) 35, 119
BsrFI RCCGGY 1 cut(s) 465
BssAI RCCGGY 1 cut(s) 465
BssECI CCNNGG 5 cut(s) 39, 74, 207, 522, 644
BssNI GRCGYC 2 cut(s) 36, 120
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 5 cut(s) 17, 103, 175, 333, 495
BstACI GRCGYC 2 cut(s) 36, 120
BstC8I GCNNGC 4 cut(s) 189, 214, 354, 603
BstDEI CTNAG 2 cut(s) 429, 690
BstDSI CCRYGG 3 cut(s) 39, 207, 644
BstEII GGTNACC 1 cut(s) 542
BstF5I GGATG 2 cut(s) 459, 654
BstFNI CGCG 8 cut(s) 41, 66, 193, 356, 380, 390, 515, 593
BstH2I RGCGCY 2 cut(s) 39, 123
BstHHI GCGC 5 cut(s) 38, 122, 193, 392, 593
BstMAI GTCTC 1 cut(s) 453
BstMCI CGRYCG 2 cut(s) 70, 614
BstMWI GCNNNNNNNGC 2 cut(s) 218, 442
BstNI CCWGG 1 cut(s) 398
BstPI GGTNACC 1 cut(s) 542
BstSCI CCNGG 5 cut(s) 74, 396, 528, 546, 669
BstSLI GKGCMC 2 cut(s) 251, 607
BstUI CGCG 8 cut(s) 41, 66, 193, 356, 380, 390, 515, 593
BstV2I GAAGAC 1 cut(s) 165
BstZI CGGCCG 1 cut(s) 611
BsuRI GGCC 6 cut(s) 206, 249, 521, 528, 605, 613
BtgI CCRYGG 3 cut(s) 39, 207, 644
BtrI CACGTC 1 cut(s) 480
BtsCI GGATG 2 cut(s) 459, 654
BtsIMutI CAGTG 3 cut(s) 99, 426, 491
Cac8I GCNNGC 4 cut(s) 189, 214, 354, 603
CfoI GCGC 5 cut(s) 38, 122, 193, 392, 593
Cfr10I RCCGGY 1 cut(s) 465
Cfr13I GGNCC 8 cut(s) 78, 108, 171, 247, 248, 526, 603, 604
Cfr42I CCGCGG 1 cut(s) 42
CseI GACGC 2 cut(s) 227, 396
Csp6I GTAC 6 cut(s) 85, 95, 334, 499, 511, 667
CspAI ACCGGT 1 cut(s) 465
CviAII CATG 2 cut(s) 202, 439
CviQI GTAC 6 cut(s) 85, 95, 334, 499, 511, 667
DdeI CTNAG 2 cut(s) 429, 690
DinI GGCGCC 2 cut(s) 37, 121
DraIII CACNNNGTG 1 cut(s) 432
DriI GACNNNNNGTC 1 cut(s) 392
EaeI YGGCCR 2 cut(s) 204, 611
EagI CGGCCG 1 cut(s) 611
Eam1105I GACNNNNNGTC 1 cut(s) 392
Ecl136II GAGCTC 3 cut(s) 91, 199, 505
EclXI CGGCCG 1 cut(s) 611
Eco130I CCWWGG 1 cut(s) 522
Eco24I GRGCYC 5 cut(s) 93, 201, 251, 507, 607
Eco47I GGWCC 3 cut(s) 78, 108, 171
Eco52I CGGCCG 1 cut(s) 611
Eco53kI GAGCTC 3 cut(s) 91, 199, 505
Eco91I GGTNACC 1 cut(s) 542
EcoICRI GAGCTC 3 cut(s) 91, 199, 505
EcoO109I RGGNCCY 1 cut(s) 604
EcoO65I GGTNACC 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 396
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 5 cut(s) 93, 201, 251, 507, 607
EgeI GGCGCC 2 cut(s) 37, 121
EheI GGCGCC 2 cut(s) 37, 121
ErhI CCWWGG 1 cut(s) 522
Esp3I CGTCTC 1 cut(s) 453
FaeI CATG 2 cut(s) 205, 442
FaiI YATR 6 cut(s) 203, 236, 290, 374, 440, 598
FaqI GGGAC 2 cut(s) 184, 641
FatI CATG 2 cut(s) 201, 438
FauI CCCGC 3 cut(s) 205, 373, 594
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 2 cut(s) 34, 39
FokI GGATG 2 cut(s) 466, 661
FriOI GRGCYC 5 cut(s) 93, 201, 251, 507, 607
Fsp4HI GCNGC 2 cut(s) 34, 39
GlaI GCGC 5 cut(s) 37, 121, 192, 391, 592
GluI GCNGC 2 cut(s) 34, 39
GsaI CCCAGC 1 cut(s) 248
GsuI CTGGAG 1 cut(s) 428
HaeII RGCGCY 2 cut(s) 39, 123
HaeIII GGCC 6 cut(s) 206, 249, 521, 528, 605, 613
HapII CCGG 8 cut(s) 76, 106, 341, 466, 529, 547, 610, 671
HgaI GACGC 2 cut(s) 227, 396
HhaI GCGC 5 cut(s) 38, 122, 193, 392, 593
Hin1I GRCGYC 2 cut(s) 36, 120
Hin1II CATG 2 cut(s) 205, 442
Hin6I GCGC 5 cut(s) 36, 120, 191, 390, 591
HinP1I GCGC 5 cut(s) 36, 120, 191, 390, 591
HinfI GANTC 2 cut(s) 415, 653
HpaII CCGG 8 cut(s) 76, 106, 341, 466, 529, 547, 610, 671
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 5 cut(s) 97, 148, 267, 386, 491
Hpy188I TCNGA 2 cut(s) 26, 453
Hpy188III TCNNGA 4 cut(s) 106, 341, 535, 682
Hpy8I GTNNAC 5 cut(s) 97, 148, 267, 386, 491
Hpy99I CGWCG 4 cut(s) 128, 179, 221, 484
HpyAV CCTTC 2 cut(s) 301, 316
HpyCH4III ACNGT 5 cut(s) 17, 103, 175, 333, 495
HpyCH4IV ACGT 2 cut(s) 458, 479
HpyCH4V TGCA 3 cut(s) 12, 372, 463
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 442
HpyF3I CTNAG 2 cut(s) 429, 690
HpySE526I ACGT 2 cut(s) 458, 479
Hsp92I GRCGYC 2 cut(s) 36, 120
Hsp92II CATG 2 cut(s) 205, 442
HspAI GCGC 5 cut(s) 36, 120, 191, 390, 591
KasI GGCGCC 2 cut(s) 35, 119
Kpn2I TCCGGA 2 cut(s) 105, 340
KspI CCGCGG 1 cut(s) 42
LmnI GCTCC 2 cut(s) 9, 442
MaeII ACGT 2 cut(s) 458, 479
MaeIII GTNAC 2 cut(s) 542, 584
MboII GAAGA 5 cut(s) 38, 170, 316, 410, 424
MhlI GDGCHC 6 cut(s) 93, 201, 251, 366, 507, 607
Mly113I GGCGCC 2 cut(s) 36, 120
MlyI GAGTC 1 cut(s) 647
MnlI CCTC 4 cut(s) 17, 511, 531, 617
MroI TCCGGA 2 cut(s) 105, 340
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 1 cut(s) 579
MslI CAYNNNNRTG 2 cut(s) 206, 381
MspA1I CMGCKG 1 cut(s) 41
MspI CCGG 8 cut(s) 76, 106, 341, 466, 529, 547, 610, 671
MspR9I CCNGG 5 cut(s) 76, 398, 530, 548, 671
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 8 cut(s) 41, 66, 193, 356, 380, 390, 515, 593
MwoI GCNNNNNNNGC 2 cut(s) 218, 442
NarI GGCGCC 2 cut(s) 36, 120
NciI CCSGG 4 cut(s) 76, 530, 548, 671
NlaIII CATG 2 cut(s) 205, 442
NlaIV GGNNCC 9 cut(s) 37, 62, 110, 121, 172, 249, 444, 605, 641
NmuCI GTSAC 1 cut(s) 542
OliI CACNNNNGTG 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 1 cut(s) 415
Pfl23II CGTACG 1 cut(s) 510
PfoI TCCNGGA 1 cut(s) 396
PinAI ACCGGT 1 cut(s) 465
PkrI GCNGC 2 cut(s) 35, 40
PleI GAGTC 1 cut(s) 647
PluTI GGCGCC 2 cut(s) 39, 123
PpsI GAGTC 1 cut(s) 647
Psp124BI GAGCTC 3 cut(s) 93, 201, 507
Psp6I CCWGG 1 cut(s) 396
PspEI GGTNACC 1 cut(s) 542
PspFI CCCAGC 1 cut(s) 244
PspGI CCWGG 1 cut(s) 396
PspLI CGTACG 1 cut(s) 510
PspN4I GGNNCC 9 cut(s) 37, 62, 110, 121, 172, 249, 444, 605, 641
PspOMI GGGCCC 2 cut(s) 247, 603
PspPI GGNCC 8 cut(s) 78, 108, 171, 247, 248, 526, 603, 604
RsaI GTAC 6 cut(s) 86, 96, 335, 500, 512, 668
RsaNI GTAC 6 cut(s) 85, 95, 334, 499, 511, 667
RseI CAYNNNNRTG 2 cut(s) 206, 381
SacI GAGCTC 3 cut(s) 93, 201, 507
SacII CCGCGG 1 cut(s) 42
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 2 cut(s) 34, 39
Sau96I GGNCC 8 cut(s) 78, 108, 171, 247, 248, 526, 603, 604
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 5 cut(s) 76, 398, 530, 548, 671
SduI GDGCHC 6 cut(s) 93, 201, 251, 366, 507, 607
SfoI GGCGCC 2 cut(s) 37, 121
Sfr303I CCGCGG 1 cut(s) 42
SgrBI CCGCGG 1 cut(s) 42
SinI GGWCC 3 cut(s) 78, 108, 171
SmiMI CAYNNNNRTG 2 cut(s) 206, 381
SspDI GGCGCC 2 cut(s) 35, 119
SstI GAGCTC 3 cut(s) 93, 201, 507
StyD4I CCNGG 5 cut(s) 74, 396, 528, 546, 669
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 5 cut(s) 17, 103, 175, 333, 495
TaiI ACGT 2 cut(s) 461, 482
TaqI TCGA 4 cut(s) 53, 360, 482, 656
TauI GCSGC 2 cut(s) 36, 41
TfiI GAWTC 1 cut(s) 415
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 3 cut(s) 106, 433, 498
TseFI GTSAC 1 cut(s) 542
Tsp45I GTSAC 1 cut(s) 542
TspDTI ATGAA 1 cut(s) 277
TspRI CASTG 3 cut(s) 106, 433, 498
VpaK11BI GGWCC 3 cut(s) 78, 108, 171
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.