Rh4DG019700

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012768
Physical Location & Seq
Reverse (-)
3031517 .. 3034481
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG019700.1

Sequence Viewer

Length: 1590 bp
ATGACTACACAAGCGCCGGAGACGTCGTCGTCGTCGTCGTCATCCTCGCCCTCGCAGGAAAACGATGTCCATCATGTAGTAACTAAGAGAGTACTTCCGAATTTTCTTCTATCCGTGAGGCTCAAGTACGTGAAGCTCGGTTACCATTACCTGATCTCCAACGCCATGTACATCTTACTCGTCCCGCTCCTTGCGGTGGCATTTGCTCATCTCTCAACCCTAACCGTCGAGGATTTGGCTCGTTTGTGGGACCAGCTCAAGTACAACTTCGTCTCCGTCACCGTCTGCTCCAGCCTCATAGTCTTCTTGACCACCGTCTACTTCACCACCCGGCCGAGGAAGGTGTACTTGGTTAACTTTGCGTGTTACAAGCCCGAGAACGAGCGCATGTGCACTAGGGAGATCTTCATGCACAGGTCCAAGCTGACGGGGAGCTTCTCCGATGAGAACCTGGCGTTCCAGCAAAAGATTCTGGAGCGCTCTGGCCTCGGGCAGAACACGTATATGCCCGAGGCCGTGCTGAGGGTTCCGCCAAACCCATGTATGGCGGAGGCCAGGAAGGAGGCGGAGATGGTGATGTTTGGGGCTATTGATGAGCTGCTGGAGAAGACGGGAGTGAAGCCTAAGGATATTGGGATTCTGGTGGTGAATTGCAGCTTGTTCAATCCAACGCCGTCTCTATCCTCCATGGTTGTGAATCATTACAAGCTTAGAGGGAATATTGTGAGCTACAATCTTGGTGGGATGGGTTGCAGTGCTGGCCTCATTTCTATTGATCTTGCCAAGCAGCTTCTCCAGGTGCACCCCAACTCATATGCCCTAGTGGTCAGCATGGAGAACATAACCCTCAATTGGTACTTTGGCAATGACCGCTCTATGCTCCTCTCGAACTGCCTCTTCCGACTTGGCGGTGCTGCCATCCTCCTCTCCAACCGCTCCTCTGACCGCCGCCGCTCCAAGTACCAACTCATGCACACCGTTCGCACCCACAAAGGCTCGGACGACAAGGCGTATGGCTGTGTTTTCCAAAAGGAAGATGATACCAAAAGAATCGGTGTAGCCCTCTCAAAAGACCTAATGGCTGTAGCCGGCGAGGCCCTCAAAACGAACATCACTACACTCGGCCCTATAGTCCTACCCATGTCAGAACAACTCCTGTTTTTTGCTACTTTGGTAGCAAGAAAAGTGTTCAAGATGAAGAAGATCAAACCCTACATCCCTGATTTCAAGTTGGCATTCGAGCACTTCTGTATCCATGCTGGTGGGAGAGCAGTGTTGGATGAGATGGAGAAGAACCTTGAGCTCACTGATTGGCATATGGAGCCTTCAAGAATGACACTTAACAGGTTTGGGAACACTTCTAGTAGTTCATTGTGGTATGAACTGGCTTACTCTGAGGCTAAGGGAAGAGTGAGAAAGGGACATAGGATGTGGCAGATTGCATTCGGGTCAGGGTTCAAGTGTAACAGTGCTGTGTGGAAGGCATTGAAGACTGTCAATCCAGCAAAGGAGAAGAATCCTTGGATTGATGAGATTCATCTGTTTCCTGTTGAGGTGCCTAAAGTGGCAGCCATTAAAATTTCTTCTTAA

Protein Analysis

529

Amino Acids

59.56

Weight (kDa)

9.35

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 107 - 396 3.3e-144 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 414 - 494 9.9e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 416 - 490 5.8e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012768)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04220 AT5G43760
fragaria_vesca FvH4_4g01980 FvH4_7g17470
malus_domestica MD13G1203100.v1.1
prunus_persica Prupe.1G025600_v2.0.a1
pyrus_communis pycom07g15420 pycom13g17640 pycom16g17200
rosa_chinensis RchiOBHm_Chr4g0388941
rosa_laevigata RLG00000010005
rosa_multiflora Rmu_sc0005486.1_g000002
rosa_roxburghii Rroxscaffold_5G00335260
rosa_rugosa Rorug03G0321500
rosa_samantha Rh4AG025900 Rh4BG019200 Rh4DG019700
rosa_wichuraiana Rw4G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 28
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 1555
AccBSI CCGCTC 4 cut(s) 187, 873, 936, 954
AccI GTMKAC 1 cut(s) 318
AcoI YGGCCR 1 cut(s) 332
AcsI RAATTY 2 cut(s) 100, 1578
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 7 cut(s) 93, 128, 170, 263, 347, 857, 962
AfeI AGCGCT 1 cut(s) 479
AfiI CCNNNNNNNGG 5 cut(s) 196, 336, 522, 544, 852
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 6 cut(s) 664, 1192, 1228, 1329, 1459, 1489
AjnI CCWGG 3 cut(s) 450, 554, 795
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
Alw21I GWGCWC 4 cut(s) 395, 804, 1245, 1305
Alw26I GTCTC 3 cut(s) 14, 277, 681
Alw44I GTGCAC 2 cut(s) 391, 800
Ama87I CYCGRG 3 cut(s) 374, 488, 509
Aor51HI AGCGCT 1 cut(s) 479
AoxI GGCC 7 cut(s) 332, 484, 513, 552, 760, 1095, 1123
ApaLI GTGCAC 2 cut(s) 391, 800
ApeKI GCWGC 5 cut(s) 598, 654, 787, 914, 1568
ApoI RAATTY 2 cut(s) 100, 1578
AspLEI GCGC 3 cut(s) 16, 387, 480
AspS9I GGNCC 4 cut(s) 250, 417, 1096, 1124
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 4 cut(s) 271, 316, 586, 658
AvaI CYCGRG 3 cut(s) 374, 488, 509
AvaII GGWCC 2 cut(s) 250, 417
AxyI CCTNAGG 1 cut(s) 624
BaeGI GKGCMC 2 cut(s) 395, 804
BaeI ACNNNNGTAYC 2 cut(s) 1234, 1267
BanI GGYRCC 1 cut(s) 1555
BanII GRGCYC 1 cut(s) 1305
BarI GAAGNNNNNNTAC 2 cut(s) 125, 157
BbsI GAAGAC 3 cut(s) 295, 614, 1496
Bbv12I GWGCWC 4 cut(s) 395, 804, 1245, 1305
BbvCI CCTCAGC 1 cut(s) 521
BbvI GCAGC 5 cut(s) 585, 666, 799, 901, 1580
BccI CCATC 5 cut(s) 78, 565, 739, 926, 1279
BceAI ACGGC 2 cut(s) 500, 658
BcgI CGANNNNNNTGC 2 cut(s) 962, 996
BciT130I CCWGG 3 cut(s) 452, 556, 797
BciVI GTATCC 1 cut(s) 1262
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 3 cut(s) 14, 277, 681
BfaI CTAG 3 cut(s) 396, 821, 1362
BfmI CTRYAG 2 cut(s) 1083, 1128
BfoI RGCGCY 2 cut(s) 17, 481
BfuI GTATCC 1 cut(s) 1262
BglI GCCNNNNNGGC 1 cut(s) 1094
BglII AGATCT 1 cut(s) 402
BisI GCNGC 7 cut(s) 599, 655, 788, 915, 949, 952, 1569
BlsI GCNGC 7 cut(s) 600, 656, 789, 916, 950, 953, 1570
BmcAI AGTACT 1 cut(s) 93
Bme1390I CCNGG 4 cut(s) 331, 452, 556, 797
Bme18I GGWCC 2 cut(s) 250, 417
BmeT110I CYCGRG 3 cut(s) 374, 488, 509
BmgT120I GGNCC 4 cut(s) 250, 417, 1096, 1124
BmiI GGNNCC 4 cut(s) 251, 528, 1323, 1557
BmrFI CCNGG 4 cut(s) 331, 452, 556, 797
BoxI GACNNNNGTC 1 cut(s) 314
BpiI GAAGAC 3 cut(s) 295, 614, 1496
BpmI CTGGAG 4 cut(s) 274, 494, 623, 779
Bpu10I CCTNAGC 2 cut(s) 521, 1401
BpuEI CTTGAG 3 cut(s) 107, 242, 1319
BpuMI CCSGG 1 cut(s) 331
BsaAI YACGTR 2 cut(s) 130, 501
BsaBI GATNNNNATC 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 335, 487, 510, 687, 1520
BsaXI ACNNNNNCTCC 6 cut(s) 11, 41, 140, 170, 257, 287
Bsc4I CCNNNNNNNGG 5 cut(s) 196, 336, 522, 544, 852
Bse118I RCCGGY 1 cut(s) 1088
Bse1I ACTGG 1 cut(s) 1389
Bse21I CCTNAGG 1 cut(s) 624
Bse3DI GCAATG 1 cut(s) 871
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 3 cut(s) 452, 556, 797
BseDI CCNNGG 5 cut(s) 335, 487, 510, 687, 1520
BseGI GGATG 6 cut(s) 41, 750, 918, 1215, 1285, 1434
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 5 cut(s) 196, 336, 522, 544, 852
BseMI GCAATG 1 cut(s) 871
BseMII CTCAG 2 cut(s) 512, 1386
BseNI ACTGG 1 cut(s) 1389
BseRI GAGGAG 3 cut(s) 872, 914, 928
BseSI GKGCMC 2 cut(s) 395, 804
BseX3I CGGCCG 1 cut(s) 332
BseXI GCAGC 5 cut(s) 585, 666, 799, 901, 1580
Bsh1285I CGRYCG 1 cut(s) 335
BshFI GGCC 7 cut(s) 334, 486, 515, 554, 762, 1097, 1125
BshNI GGYRCC 1 cut(s) 1555
BsiEI CGRYCG 1 cut(s) 335
BsiHKAI GWGCWC 4 cut(s) 395, 804, 1245, 1305
BsiHKCI CYCGRG 3 cut(s) 374, 488, 509
BsiSI CCGG 3 cut(s) 17, 331, 1089
BslFI GGGAC 3 cut(s) 167, 263, 1434
BslI CCNNNNNNNGG 5 cut(s) 196, 336, 522, 544, 852
BsmAI GTCTC 3 cut(s) 14, 277, 681
BsmBI CGTCTC 3 cut(s) 14, 277, 681
BsmFI GGGAC 3 cut(s) 167, 263, 1434
BsmI GAATGC 2 cut(s) 1235, 1442
BsnI GGCC 7 cut(s) 334, 486, 515, 554, 762, 1097, 1125
BsoBI CYCGRG 3 cut(s) 374, 488, 509
Bsp1286I GDGCHC 4 cut(s) 395, 804, 1245, 1305
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 4 cut(s) 153, 402, 775, 1203
Bsp19I CCATGG 1 cut(s) 687
BspANI GGCC 7 cut(s) 334, 486, 515, 554, 762, 1097, 1125
BspCNI CTCAG 2 cut(s) 513, 1387
BspLI GGNNCC 4 cut(s) 251, 528, 1323, 1557
BspT107I GGYRCC 1 cut(s) 1555
BsrBI CCGCTC 4 cut(s) 187, 873, 936, 954
BsrDI GCAATG 1 cut(s) 871
BsrFI RCCGGY 1 cut(s) 1088
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 1 cut(s) 1389
BssAI RCCGGY 1 cut(s) 1088
BssECI CCNNGG 5 cut(s) 335, 487, 510, 687, 1520
BssMI GATC 4 cut(s) 153, 402, 775, 1203
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 2 cut(s) 687, 1520
Bst2UI CCWGG 3 cut(s) 452, 556, 797
Bst4CI ACNGT 6 cut(s) 226, 283, 316, 979, 1469, 1495
Bst6I CTCTTC 2 cut(s) 902, 1402
BstACI GRCGYC 1 cut(s) 23
BstAUI TGTACA 1 cut(s) 168
BstBAI YACGTR 2 cut(s) 130, 501
BstC8I GCNNGC 2 cut(s) 760, 1090
BstDEI CTNAG 6 cut(s) 84, 521, 624, 710, 1395, 1401
BstDSI CCRYGG 1 cut(s) 687
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 6 cut(s) 41, 750, 918, 1215, 1285, 1434
BstH2I RGCGCY 2 cut(s) 17, 481
BstHHI GCGC 3 cut(s) 16, 387, 480
BstKTI GATC 4 cut(s) 156, 405, 778, 1206
BstMAI GTCTC 3 cut(s) 14, 277, 681
BstMBI GATC 4 cut(s) 153, 402, 775, 1203
BstMCI CGRYCG 1 cut(s) 335
BstMWI GCNNNNNNNGC 4 cut(s) 759, 870, 1094, 1321
BstNI CCWGG 3 cut(s) 452, 556, 797
BstNSI RCATGY 1 cut(s) 391
BstPAI GACNNNNGTC 1 cut(s) 314
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 4 cut(s) 329, 450, 554, 795
BstSFI CTRYAG 2 cut(s) 1083, 1128
BstSLI GKGCMC 2 cut(s) 395, 804
BstV1I GCAGC 5 cut(s) 585, 666, 799, 901, 1580
BstV2I GAAGAC 3 cut(s) 295, 614, 1496
BstX2I RGATCY 1 cut(s) 402
BstXI CCANNNNNNTGG 1 cut(s) 1262
BstYI RGATCY 1 cut(s) 402
BstZI CGGCCG 1 cut(s) 332
Bsu36I CCTNAGG 1 cut(s) 624
BsuI GTATCC 1 cut(s) 1262
BsuRI GGCC 7 cut(s) 334, 486, 515, 554, 762, 1097, 1125
BtgI CCRYGG 1 cut(s) 687
BtsCI GGATG 6 cut(s) 41, 750, 918, 1215, 1285, 1434
BtsI GCAGTG 2 cut(s) 760, 1278
BtsIMutI CAGTG 4 cut(s) 760, 1278, 1305, 1474
Cac8I GCNNGC 2 cut(s) 760, 1090
CfoI GCGC 3 cut(s) 16, 387, 480
Cfr10I RCCGGY 1 cut(s) 1088
Cfr13I GGNCC 4 cut(s) 250, 417, 1096, 1124
Csp6I GTAC 7 cut(s) 92, 127, 169, 262, 346, 856, 961
CspCI CAANNNNNGTGG 2 cut(s) 721, 756
CviQI GTAC 7 cut(s) 92, 127, 169, 262, 346, 856, 961
DdeI CTNAG 6 cut(s) 84, 521, 624, 710, 1395, 1401
DpnI GATC 4 cut(s) 155, 404, 777, 1205
DpnII GATC 4 cut(s) 153, 402, 775, 1203
DrdI GACNNNNNNGTC 1 cut(s) 28
DseDI GACNNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 1 cut(s) 332
EagI CGGCCG 1 cut(s) 332
Eam1104I CTCTTC 2 cut(s) 902, 1402
EarI CTCTTC 2 cut(s) 902, 1402
EciI GGCGGA 3 cut(s) 519, 563, 581
Ecl136II GAGCTC 1 cut(s) 1303
EclXI CGGCCG 1 cut(s) 332
Eco130I CCWWGG 2 cut(s) 687, 1520
Eco24I GRGCYC 1 cut(s) 1305
Eco47I GGWCC 2 cut(s) 250, 417
Eco47III AGCGCT 1 cut(s) 479
Eco52I CGGCCG 1 cut(s) 332
Eco53kI GAGCTC 1 cut(s) 1303
Eco81I CCTNAGG 1 cut(s) 624
Eco88I CYCGRG 3 cut(s) 374, 488, 509
Eco91I GGTNACC 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 1303
EcoO109I RGGNCCY 1 cut(s) 1096
EcoO65I GGTNACC 1 cut(s) 140
EcoRII CCWGG 3 cut(s) 450, 554, 795
EcoT14I CCWWGG 2 cut(s) 687, 1520
EcoT38I GRGCYC 1 cut(s) 1305
ErhI CCWWGG 2 cut(s) 687, 1520
Esp3I CGTCTC 3 cut(s) 14, 277, 681
FalI AAGNNNNNCTT 4 cut(s) 251, 283, 332, 364
FaqI GGGAC 3 cut(s) 167, 263, 1434
FauI CCCGC 1 cut(s) 192
FauNDI CATATG 2 cut(s) 814, 1317
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 7 cut(s) 599, 655, 788, 915, 949, 952, 1569
FokI GGATG 6 cut(s) 28, 757, 905, 1202, 1292, 1441
FriOI GRGCYC 1 cut(s) 1305
Fsp4HI GCNGC 7 cut(s) 599, 655, 788, 915, 949, 952, 1569
FspBI CTAG 3 cut(s) 396, 821, 1362
GlaI GCGC 3 cut(s) 15, 386, 479
GluI GCNGC 7 cut(s) 599, 655, 788, 915, 949, 952, 1569
GsuI CTGGAG 4 cut(s) 274, 494, 623, 779
HaeII RGCGCY 2 cut(s) 17, 481
HaeIII GGCC 7 cut(s) 334, 486, 515, 554, 762, 1097, 1125
HapII CCGG 3 cut(s) 17, 331, 1089
HhaI GCGC 3 cut(s) 16, 387, 480
Hin1I GRCGYC 1 cut(s) 23
Hin6I GCGC 3 cut(s) 14, 385, 478
HinP1I GCGC 3 cut(s) 14, 385, 478
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 6 cut(s) 469, 637, 697, 1050, 1516, 1534
HpaI GTTAAC 1 cut(s) 355
HpaII CCGG 3 cut(s) 17, 331, 1089
HphI GGTGA 4 cut(s) 271, 316, 586, 658
Hpy166II GTNNAC 5 cut(s) 319, 346, 355, 393, 802
Hpy188I TCNGA 7 cut(s) 99, 442, 902, 943, 1000, 1147, 1396
Hpy188III TCNNGA 5 cut(s) 307, 473, 886, 1192, 1329
Hpy8I GTNNAC 5 cut(s) 319, 346, 355, 393, 802
Hpy99I CGWCG 6 cut(s) 28, 31, 34, 37, 40, 230
HpyAV CCTTC 4 cut(s) 334, 553, 1335, 1474
HpyCH4III ACNGT 6 cut(s) 226, 283, 316, 979, 1469, 1495
HpyCH4IV ACGT 3 cut(s) 23, 129, 500
HpyCH4V TGCA 7 cut(s) 393, 412, 654, 753, 802, 973, 1442
HpyF10VI GCNNNNNNNGC 4 cut(s) 759, 870, 1094, 1321
HpyF3I CTNAG 6 cut(s) 84, 521, 624, 710, 1395, 1401
HpySE526I ACGT 3 cut(s) 23, 129, 500
Hsp92I GRCGYC 1 cut(s) 23
HspAI GCGC 3 cut(s) 14, 385, 478
KroI GCCGGC 1 cut(s) 1088
KroNI GCCGGC 1 cut(s) 1090
KspAI GTTAAC 1 cut(s) 355
Kzo9I GATC 4 cut(s) 153, 402, 775, 1203
LmnI GCTCC 8 cut(s) 192, 293, 432, 475, 885, 941, 959, 1321
Lsp1109I GCAGC 5 cut(s) 585, 666, 799, 901, 1580
MaeI CTAG 3 cut(s) 396, 821, 1362
MaeII ACGT 3 cut(s) 23, 129, 500
MaeIII GTNAC 5 cut(s) 79, 140, 277, 365, 1463
MalI GATC 4 cut(s) 155, 404, 777, 1205
MbiI CCGCTC 4 cut(s) 187, 873, 936, 954
MboI GATC 4 cut(s) 153, 402, 775, 1203
MfeI CAATTG 1 cut(s) 850
MflI RGATCY 1 cut(s) 402
MhlI GDGCHC 4 cut(s) 395, 804, 1245, 1305
MluCI AATT 4 cut(s) 100, 649, 850, 1578
MmeI TCCRAC 5 cut(s) 183, 692, 925, 954, 1257
MroNI GCCGGC 1 cut(s) 1088
MseI TTAA 4 cut(s) 354, 1341, 1575, 1588
MslI CAYNNNNRTG 3 cut(s) 503, 692, 1260
MspI CCGG 3 cut(s) 17, 331, 1089
MspR9I CCNGG 4 cut(s) 331, 452, 556, 797
MunI CAATTG 1 cut(s) 850
Mva1269I GAATGC 2 cut(s) 1235, 1442
MvaI CCWGG 3 cut(s) 452, 556, 797
MwoI GCNNNNNNNGC 4 cut(s) 759, 870, 1094, 1321
NaeI GCCGGC 1 cut(s) 1090
NciI CCSGG 1 cut(s) 331
NcoI CCATGG 1 cut(s) 687
NdeI CATATG 2 cut(s) 814, 1317
NdeII GATC 4 cut(s) 153, 402, 775, 1203
NgoMIV GCCGGC 1 cut(s) 1088
NlaIV GGNNCC 4 cut(s) 251, 528, 1323, 1557
NmeAIII GCCGAG 2 cut(s) 360, 1101
NmuCI GTSAC 1 cut(s) 277
NspI RCATGY 1 cut(s) 391
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 32, 35
PctI GAATGC 2 cut(s) 1235, 1442
PdiI GCCGGC 1 cut(s) 1090
PfeI GAWTC 6 cut(s) 469, 637, 697, 1050, 1516, 1534
PflFI GACNNNGTC 1 cut(s) 25
PkrI GCNGC 7 cut(s) 600, 656, 789, 916, 950, 953, 1570
Ppu21I YACGTR 2 cut(s) 130, 501
PshAI GACNNNNGTC 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 1305
Psp6I CCWGG 3 cut(s) 450, 554, 795
PspEI GGTNACC 1 cut(s) 140
PspGI CCWGG 3 cut(s) 450, 554, 795
PspN4I GGNNCC 4 cut(s) 251, 528, 1323, 1557
PspPI GGNCC 4 cut(s) 250, 417, 1096, 1124
PsuI RGATCY 1 cut(s) 402
PsyI GACNNNGTC 1 cut(s) 25
RsaI GTAC 7 cut(s) 93, 128, 170, 263, 347, 857, 962
RsaNI GTAC 7 cut(s) 92, 127, 169, 262, 346, 856, 961
RseI CAYNNNNRTG 3 cut(s) 503, 692, 1260
SacI GAGCTC 1 cut(s) 1305
SaqAI TTAA 4 cut(s) 354, 1341, 1575, 1588
SatI GCNGC 7 cut(s) 599, 655, 788, 915, 949, 952, 1569
Sau3AI GATC 4 cut(s) 153, 402, 775, 1203
Sau96I GGNCC 4 cut(s) 250, 417, 1096, 1124
ScaI AGTACT 1 cut(s) 93
ScrFI CCNGG 4 cut(s) 331, 452, 556, 797
SduI GDGCHC 4 cut(s) 395, 804, 1245, 1305
SfcI CTRYAG 2 cut(s) 1083, 1128
SinI GGWCC 2 cut(s) 250, 417
SmiMI CAYNNNNRTG 3 cut(s) 503, 692, 1260
SmlI CTYRAG 3 cut(s) 122, 257, 1298
SmoI CTYRAG 3 cut(s) 122, 257, 1298
Sse9I AATT 4 cut(s) 100, 649, 850, 1578
SspI AATATT 1 cut(s) 721
SspMI CTAG 3 cut(s) 396, 821, 1362
SstI GAGCTC 1 cut(s) 1305
StyD4I CCNGG 4 cut(s) 329, 450, 554, 795
StyI CCWWGG 2 cut(s) 687, 1520
TaaI ACNGT 6 cut(s) 226, 283, 316, 979, 1469, 1495
TaiI ACGT 3 cut(s) 26, 132, 503
TaqI TCGA 3 cut(s) 228, 887, 1239
TasI AATT 4 cut(s) 100, 649, 850, 1578
TatI WGTACW 4 cut(s) 91, 168, 261, 345
TauI GCSGC 2 cut(s) 951, 954
TfiI GAWTC 6 cut(s) 469, 637, 697, 1050, 1516, 1534
Tru1I TTAA 4 cut(s) 354, 1341, 1575, 1588
Tru9I TTAA 4 cut(s) 354, 1341, 1575, 1588
TscAI CASTG 4 cut(s) 760, 1278, 1312, 1474
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 5 cut(s) 598, 654, 787, 914, 1568
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 5 cut(s) 397, 1211, 1359, 1395, 1526
TspGWI ACGGA 2 cut(s) 103, 265
TspRI CASTG 4 cut(s) 760, 1278, 1312, 1474
Tth111I GACNNNGTC 1 cut(s) 25
VneI GTGCAC 2 cut(s) 391, 800
VpaK11BI GGWCC 2 cut(s) 250, 417
XapI RAATTY 2 cut(s) 100, 1578
XceI RCATGY 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 318
XspI CTAG 3 cut(s) 396, 821, 1362
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.