pycom16g17200

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012768
Physical Location & Seq
Reverse (-)
13279413 .. 13280204
792 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g17200.1

Sequence Viewer

Length: 792 bp
ATGGCGGCACAAAGCCAGAACCAGAAAGGAGAATCGAACTCGGGACCTGAGTCAGCAGGAAGTCGCAAAACTACGCTTCCTAACTTTCTTTTATCAGTGAAGCTCAAATATGTCAAGCTAGGGTACCACTACCTAATCTCCAACGTCATCTACCTCCTACTAGTCCCACTCATCGCCGCGGCCTCCACCCACTTCTCGACGCTCACCGCCAATGACTTCCTCCACTTGTGGCAAAAGCTCAAGTTCAACTTCGTCTCGGTCACCTTGTGCTCGGGCCTCATGGTTTTCCTAACCACCGTATACTTCACCACCCGCCCTAGAAAAGTATACTTGGTGAACTTCGCATGCTACAAGCTCGAGCCGGCCCGCACGTGCACATGTGAGATATTCATGGAGAGGTCGAAGCTGACGGGCAGCTTCTCTGACGAGAACTTGGCTTTTCAGCAAAAGATTCTCGAGAGGTCCGGCCTCGGGCAAAACACATACATACCCGAGGCCTTGCTCCAGGTCCCTCCAAACTCGTGCATGGCGGAGGCGAGGAAGGAGGCGGAAATGGTGATGTTCGGAGCGATAGACGAGTTGCTGGAGAAGACGAGGGTGAAGCCTAAGGATATTGGGATTTTGGTGGTGAACTGCAGCTTGTTCAATCCGACGCCGTCTCTGTCGTCCATGGTTGTGAATCACTATAAACTCAGGGGGAACGTTGTGAGCTATAACCTTGGTGGGATGGGTTGCAGTGCTGGCCTCATTTCTATTGACCTGGCAAAGCAGCTTCTCCAGGTTTGTTACTAA

Protein Analysis

264

Amino Acids

29.22

Weight (kDa)

9.04

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 101 - 261 9.3e-72 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012768)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04220 AT5G43760
fragaria_vesca FvH4_4g01980 FvH4_7g17470
malus_domestica MD13G1203100.v1.1
prunus_persica Prupe.1G025600_v2.0.a1
pyrus_communis pycom07g15420 pycom13g17640 pycom16g17200
rosa_chinensis RchiOBHm_Chr4g0388941
rosa_laevigata RLG00000010005
rosa_multiflora Rmu_sc0005486.1_g000002
rosa_roxburghii Rroxscaffold_5G00335260
rosa_rugosa Rorug03G0321500
rosa_samantha Rh4AG025900 Rh4BG019200 Rh4DG019700
rosa_wichuraiana Rw4G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 123
AccB1I GGYRCC 1 cut(s) 123
AccI GTMKAC 2 cut(s) 300, 327
AccII CGCG 1 cut(s) 179
AciI CCGC 8 cut(s) 5, 177, 179, 207, 313, 367, 530, 548
AclI AACGTT 1 cut(s) 702
AcvI CACGTG 1 cut(s) 372
AcyI GRCGYC 1 cut(s) 653
AdeI CACNNNGTG 1 cut(s) 267
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 471
AflIII ACRYGT 1 cut(s) 377
AgsI TTSAA 2 cut(s) 247, 646
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 3 cut(s) 504, 759, 777
AluBI AGCT 9 cut(s) 103, 118, 238, 355, 406, 417, 639, 711, 772
AluI AGCT 9 cut(s) 103, 118, 238, 355, 406, 417, 639, 711, 772
Alw21I GWGCWC 2 cut(s) 272, 377
Alw26I GTCTC 2 cut(s) 259, 663
Alw44I GTGCAC 1 cut(s) 373
Ama87I CYCGRG 6 cut(s) 40, 271, 356, 455, 470, 491
AoxI GGCC 6 cut(s) 180, 274, 363, 466, 495, 742
ApaLI GTGCAC 1 cut(s) 373
ApeKI GCWGC 3 cut(s) 414, 636, 769
Asp718I GGTACC 1 cut(s) 123
AspS9I GGNCC 5 cut(s) 44, 274, 364, 462, 508
AsuHPI GGTGA 7 cut(s) 196, 253, 298, 346, 568, 610, 640
AvaI CYCGRG 6 cut(s) 40, 271, 356, 455, 470, 491
AvaII GGWCC 3 cut(s) 44, 462, 508
AxyI CCTNAGG 1 cut(s) 606
BaeGI GKGCMC 1 cut(s) 377
BanI GGYRCC 1 cut(s) 123
BauI CACGAG 1 cut(s) 520
BbrPI CACGTG 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 596
Bbv12I GWGCWC 2 cut(s) 272, 377
BbvI GCAGC 3 cut(s) 426, 648, 781
BccI CCATC 1 cut(s) 721
BceAI ACGGC 1 cut(s) 640
BciT130I CCWGG 3 cut(s) 506, 761, 779
BcoDI GTCTC 2 cut(s) 259, 663
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 3 cut(s) 119, 161, 318
BfmI CTRYAG 1 cut(s) 634
BisI GCNGC 6 cut(s) 6, 177, 180, 415, 637, 770
BlsI GCNGC 6 cut(s) 7, 178, 181, 416, 638, 771
Bme1390I CCNGG 3 cut(s) 506, 761, 779
Bme18I GGWCC 3 cut(s) 44, 462, 508
BmeT110I CYCGRG 6 cut(s) 40, 271, 356, 455, 470, 491
BmgT120I GGNCC 5 cut(s) 44, 274, 364, 462, 508
BmiI GGNNCC 3 cut(s) 45, 125, 510
BmrFI CCNGG 3 cut(s) 506, 761, 779
BoxI GACNNNNGTC 1 cut(s) 49
BpiI GAAGAC 1 cut(s) 596
BpmI CTGGAG 3 cut(s) 488, 605, 761
BpuEI CTTGAG 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 653
BsaJI CCNNGG 5 cut(s) 177, 469, 492, 669, 718
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 1 cut(s) 471
Bse118I RCCGGY 1 cut(s) 361
Bse21I CCTNAGG 1 cut(s) 606
BseBI CCWGG 3 cut(s) 506, 761, 779
BseDI CCNNGG 5 cut(s) 177, 469, 492, 669, 718
BseGI GGATG 1 cut(s) 732
BseLI CCNNNNNNNGG 1 cut(s) 471
BseMII CTCAG 2 cut(s) 39, 706
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 3 cut(s) 426, 648, 781
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 6 cut(s) 182, 276, 365, 468, 497, 744
BshNI GGYRCC 1 cut(s) 123
BsiHKAI GWGCWC 2 cut(s) 272, 377
BsiHKCI CYCGRG 6 cut(s) 40, 271, 356, 455, 470, 491
BsiSI CCGG 2 cut(s) 362, 465
BslFI GGGAC 3 cut(s) 57, 149, 494
BslI CCNNNNNNNGG 1 cut(s) 471
BsmAI GTCTC 2 cut(s) 259, 663
BsmBI CGTCTC 2 cut(s) 259, 663
BsmFI GGGAC 3 cut(s) 57, 149, 494
BsnI GGCC 6 cut(s) 182, 276, 365, 468, 497, 744
BsoBI CYCGRG 6 cut(s) 40, 271, 356, 455, 470, 491
Bsp1286I GDGCHC 2 cut(s) 272, 377
Bsp19I CCATGG 1 cut(s) 669
BspACI CCGC 8 cut(s) 5, 177, 179, 207, 313, 367, 530, 548
BspANI GGCC 6 cut(s) 182, 276, 365, 468, 497, 744
BspCNI CTCAG 2 cut(s) 40, 705
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 3 cut(s) 45, 125, 510
BspMAI CTGCAG 1 cut(s) 638
BspT107I GGYRCC 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 361
BssAI RCCGGY 1 cut(s) 361
BssECI CCNNGG 5 cut(s) 177, 469, 492, 669, 718
BssNAI GTATAC 2 cut(s) 301, 328
BssNI GRCGYC 1 cut(s) 653
BssSI CACGAG 1 cut(s) 520
BssT1I CCWWGG 2 cut(s) 669, 718
Bst1107I GTATAC 2 cut(s) 301, 328
Bst2BI CACGAG 1 cut(s) 520
Bst2UI CCWGG 3 cut(s) 506, 761, 779
Bst4CI ACNGT 1 cut(s) 298
BstACI GRCGYC 1 cut(s) 653
BstBAI YACGTR 1 cut(s) 372
BstC8I GCNNGC 4 cut(s) 346, 363, 367, 742
BstDEI CTNAG 3 cut(s) 48, 606, 692
BstDSI CCRYGG 2 cut(s) 177, 669
BstEII GGTNACC 1 cut(s) 259
BstF5I GGATG 1 cut(s) 732
BstFNI CGCG 1 cut(s) 179
BstMAI GTCTC 2 cut(s) 259, 663
BstMWI GCNNNNNNNGC 1 cut(s) 741
BstNI CCWGG 3 cut(s) 506, 761, 779
BstNSI RCATGY 2 cut(s) 348, 381
BstPAI GACNNNNGTC 1 cut(s) 49
BstPI GGTNACC 1 cut(s) 259
BstSCI CCNGG 3 cut(s) 504, 759, 777
BstSFI CTRYAG 1 cut(s) 634
BstSLI GKGCMC 1 cut(s) 377
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 426, 648, 781
BstV2I GAAGAC 1 cut(s) 596
BstZ17I GTATAC 2 cut(s) 301, 328
Bsu36I CCTNAGG 1 cut(s) 606
BsuRI GGCC 6 cut(s) 182, 276, 365, 468, 497, 744
BtgI CCRYGG 2 cut(s) 177, 669
BtgZI GCGATG 1 cut(s) 157
BtsCI GGATG 1 cut(s) 732
BtsI GCAGTG 1 cut(s) 742
BtsIMutI CAGTG 2 cut(s) 102, 742
Cac8I GCNNGC 4 cut(s) 346, 363, 367, 742
Cfr10I RCCGGY 1 cut(s) 361
Cfr13I GGNCC 5 cut(s) 44, 274, 364, 462, 508
Cfr42I CCGCGG 1 cut(s) 180
CseI GACGC 2 cut(s) 208, 661
Csp6I GTAC 1 cut(s) 124
CviAII CATG 6 cut(s) 280, 345, 378, 391, 526, 670
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 3 cut(s) 48, 606, 692
DraIII CACNNNGTG 1 cut(s) 267
EciI GGCGGA 2 cut(s) 545, 563
Eco130I CCWWGG 2 cut(s) 669, 718
Eco147I AGGCCT 1 cut(s) 497
Eco47I GGWCC 3 cut(s) 44, 462, 508
Eco72I CACGTG 1 cut(s) 372
Eco81I CCTNAGG 1 cut(s) 606
Eco88I CYCGRG 6 cut(s) 40, 271, 356, 455, 470, 491
Eco91I GGTNACC 1 cut(s) 259
EcoO109I RGGNCCY 2 cut(s) 44, 508
EcoO65I GGTNACC 1 cut(s) 259
EcoRII CCWGG 3 cut(s) 504, 759, 777
EcoT14I CCWWGG 2 cut(s) 669, 718
ErhI CCWWGG 2 cut(s) 669, 718
Esp3I CGTCTC 2 cut(s) 259, 663
FaeI CATG 6 cut(s) 283, 348, 381, 394, 529, 673
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 3 cut(s) 57, 149, 494
FatI CATG 6 cut(s) 279, 344, 377, 390, 525, 669
FauI CCCGC 2 cut(s) 320, 374
FblI GTMKAC 2 cut(s) 300, 327
Fnu4HI GCNGC 6 cut(s) 6, 177, 180, 415, 637, 770
FokI GGATG 1 cut(s) 739
Fsp4HI GCNGC 6 cut(s) 6, 177, 180, 415, 637, 770
FspBI CTAG 3 cut(s) 119, 161, 318
GluI GCNGC 6 cut(s) 6, 177, 180, 415, 637, 770
GsuI CTGGAG 3 cut(s) 488, 605, 761
HaeIII GGCC 6 cut(s) 182, 276, 365, 468, 497, 744
HapII CCGG 2 cut(s) 362, 465
HgaI GACGC 2 cut(s) 208, 661
Hin1I GRCGYC 1 cut(s) 653
Hin1II CATG 6 cut(s) 283, 348, 381, 394, 529, 673
HinfI GANTC 4 cut(s) 32, 50, 451, 679
HpaII CCGG 2 cut(s) 362, 465
HphI GGTGA 7 cut(s) 196, 253, 298, 346, 568, 610, 640
Hpy166II GTNNAC 5 cut(s) 301, 328, 337, 375, 631
Hpy188I TCNGA 3 cut(s) 424, 566, 651
Hpy188III TCNNGA 4 cut(s) 42, 196, 455, 457
Hpy8I GTNNAC 5 cut(s) 301, 328, 337, 375, 631
Hpy99I CGWCG 2 cut(s) 202, 655
HpyAV CCTTC 1 cut(s) 535
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 3 cut(s) 144, 371, 702
HpyCH4V TGCA 4 cut(s) 375, 525, 636, 735
HpyF10VI GCNNNNNNNGC 1 cut(s) 741
HpyF3I CTNAG 3 cut(s) 48, 606, 692
HpySE526I ACGT 3 cut(s) 144, 371, 702
Hsp92I GRCGYC 1 cut(s) 653
Hsp92II CATG 6 cut(s) 283, 348, 381, 394, 529, 673
KpnI GGTACC 1 cut(s) 127
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
KspI CCGCGG 1 cut(s) 180
LmnI GCTCC 2 cut(s) 507, 566
Lsp1109I GCAGC 3 cut(s) 426, 648, 781
MaeI CTAG 3 cut(s) 119, 161, 318
MaeII ACGT 3 cut(s) 144, 371, 702
MaeIII GTNAC 2 cut(s) 259, 785
MboII GAAGA 1 cut(s) 601
MhlI GDGCHC 2 cut(s) 272, 377
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 2 cut(s) 165, 674
MroNI GCCGGC 1 cut(s) 361
MslI CAYNNNNRTG 1 cut(s) 674
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 2 cut(s) 362, 465
MspR9I CCNGG 3 cut(s) 506, 761, 779
MvaI CCWGG 3 cut(s) 506, 761, 779
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 1 cut(s) 741
NaeI GCCGGC 1 cut(s) 363
NcoI CCATGG 1 cut(s) 669
NgoMIV GCCGGC 1 cut(s) 361
NlaIII CATG 6 cut(s) 283, 348, 381, 394, 529, 673
NlaIV GGNNCC 3 cut(s) 45, 125, 510
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 2 cut(s) 348, 381
PaeI GCATGC 1 cut(s) 348
PaeR7I CTCGAG 2 cut(s) 356, 455
PceI AGGCCT 1 cut(s) 497
PciI ACATGT 1 cut(s) 377
PdiI GCCGGC 1 cut(s) 363
PfeI GAWTC 3 cut(s) 32, 451, 679
PflFI GACNNNGTC 1 cut(s) 655
PkrI GCNGC 6 cut(s) 7, 178, 181, 416, 638, 771
PleI GAGTC 1 cut(s) 58
PmaCI CACGTG 1 cut(s) 372
PmlI CACGTG 1 cut(s) 372
PpsI GAGTC 1 cut(s) 58
Ppu21I YACGTR 1 cut(s) 372
PpuMI RGGWCCY 2 cut(s) 44, 508
PscI ACATGT 1 cut(s) 377
PshAI GACNNNNGTC 1 cut(s) 49
Psp1406I AACGTT 1 cut(s) 702
Psp5II RGGWCCY 2 cut(s) 44, 508
Psp6I CCWGG 3 cut(s) 504, 759, 777
PspCI CACGTG 1 cut(s) 372
PspEI GGTNACC 1 cut(s) 259
PspGI CCWGG 3 cut(s) 504, 759, 777
PspN4I GGNNCC 3 cut(s) 45, 125, 510
PspPI GGNCC 5 cut(s) 44, 274, 364, 462, 508
PspPPI RGGWCCY 2 cut(s) 44, 508
PspXI VCTCGAGB 1 cut(s) 356
PstI CTGCAG 1 cut(s) 638
PsyI GACNNNGTC 1 cut(s) 655
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 674
SacII CCGCGG 1 cut(s) 180
SatI GCNGC 6 cut(s) 6, 177, 180, 415, 637, 770
Sau96I GGNCC 5 cut(s) 44, 274, 364, 462, 508
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 3 cut(s) 506, 761, 779
SduI GDGCHC 2 cut(s) 272, 377
SfcI CTRYAG 1 cut(s) 634
Sfr274I CTCGAG 2 cut(s) 356, 455
Sfr303I CCGCGG 1 cut(s) 180
SgrBI CCGCGG 1 cut(s) 180
SinI GGWCC 3 cut(s) 44, 462, 508
SlaI CTCGAG 2 cut(s) 356, 455
SmiMI CAYNNNNRTG 1 cut(s) 674
SmlI CTYRAG 3 cut(s) 239, 356, 455
SmoI CTYRAG 3 cut(s) 239, 356, 455
SpeI ACTAGT 1 cut(s) 160
SphI GCATGC 1 cut(s) 348
SseBI AGGCCT 1 cut(s) 497
SsiI CCGC 8 cut(s) 5, 177, 179, 207, 313, 367, 530, 548
SspMI CTAG 3 cut(s) 119, 161, 318
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 3 cut(s) 504, 759, 777
StyI CCWWGG 2 cut(s) 669, 718
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 3 cut(s) 147, 374, 705
TaqI TCGA 5 cut(s) 35, 197, 357, 401, 456
TaqII GACCGA 1 cut(s) 247
TauI GCSGC 3 cut(s) 8, 179, 182
TfiI GAWTC 3 cut(s) 32, 451, 679
TscAI CASTG 2 cut(s) 102, 742
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 3 cut(s) 414, 636, 769
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 379
TspRI CASTG 2 cut(s) 102, 742
Tth111I GACNNNGTC 1 cut(s) 655
VneI GTGCAC 1 cut(s) 373
VpaK11BI GGWCC 3 cut(s) 44, 462, 508
XceI RCATGY 2 cut(s) 348, 381
XhoI CTCGAG 2 cut(s) 356, 455
XmiI GTMKAC 2 cut(s) 300, 327
XspI CTAG 3 cut(s) 119, 161, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.