Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0010373

Physical Location & Seq

Genomic Coordinates Reverse (-)

19812080 .. 19813559

Gene Structure 1480 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1212400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 675 bp

ATGGGGGAATGGATAGAGTATCTTGATTACATCGACGAAACCCAACCCACCGATTTCCTTTGGCCCAACGACACCCCTTCTTTCGGCGATTTCGAATTCTCCGCCGTCGATGTTCCGGCTGCCGCCGCTTCCTCAGCCTCACAGTCACAGGAAAAGGAGTGCCCCCGCAAGAGGGGACGGACGGATTCGTGCTCTGGAGGATCAGGGGCAAAAGCTTGCCGTGAGAAATTGAGGAGGGAGAGATTGAATGACAGGTTTGTGGAGTTGAGCGGTGTTTTGGAACCCGGGAGTTCGCCGAAGACGGACAAGGCTGCTATACTTGATGATGCCGTAAGGGTTTTGAACCAGCTTAGAGCTGAAGCTCAGGAGCTCACAGAAACAAATCAAAAGTTACTTGAAGAAGTTAAAAGTTTAAAGGCAGAGAAGAATGAACTTCGTGCAGAAAAACTTGTCCTGAAAGCAGACAAAGAACGGCTGGAACAGCAGTTGAAAGGTATGGCAATTTCACCTTCTGGGTTTGTTCCAACCCACCCAGCAGTACCAGCTGCATATCATCCTGGGGCAAACAAGATGGCTGTCTATCCTAGCTATAGTCTGGTCCCTATGTGGCACTATATACCTCCATCGGCCCGTGATACATCTCGTGATCACGAACTTAGGCCCCCTGCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009743 GO:0009746 GO:0009749 GO:0009889 GO:0009987 GO:0010029 GO:0010030 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019725 GO:0031323 GO:0031326 GO:0033500 GO:0034284 GO:0042221 GO:0042592 GO:0042593 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051239 GO:0051240 GO:0051252 GO:0051716 GO:0055082 GO:0060255 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0080090 GO:0097159 GO:0140110 GO:1900140 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

225

Amino Acids

24.85

Weight (kDa)

5.53

Isoelectric Point (pI)

55.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	70 - 118	5.6e-10	Helix-loop-helix DNA-binding domain
bHLH_IRO3	PF23177	71 - 136	2.6e-07	Iron-related transcription factor 3 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23210 AT3G23210 AT4G14410 AT4G14410 AT4G14410
fragaria_vesca	FvH4_4g03420
malus_domestica	MD13G1212400.v1.1 MD16G1216000.v1.1
prunus_persica	Prupe.1G037400_v2.0.a1
pyrus_communis	pycom13g18420 pycom16g18070
rosa_chinensis	RchiOBHm_Chr4g0392401
rosa_laevigata	RLG00000009814
rosa_multiflora	Rmu_sc0001800.1_g000017
rosa_roxburghii	Rroxscaffold_5G00337580
rosa_rugosa	Rorug03G0338600
rosa_samantha	Rh4AG042400 Rh4BG037300 Rh4CG045200 Rh4DG039700
rosa_wichuraiana	Rw4G003310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	270
AciI	CCGC	5 cut(s)	102, 123, 126, 166, 270
AclWI	GGATC	1 cut(s)	208
AcsI	RAATTY	1 cut(s)	95
AcuI	CTGAAG	1 cut(s)	378
AfaI	GTAC	1 cut(s)	540
AfiI	CCNNNNNNNGG	3 cut(s)	83, 171, 172
AgsI	TTSAA	4 cut(s)	247, 343, 398, 490
AjnI	CCWGG	1 cut(s)	556
AluBI	AGCT	7 cut(s)	215, 349, 356, 362, 370, 545, 588
AluI	AGCT	7 cut(s)	215, 349, 356, 362, 370, 545, 588
Alw21I	GWGCWC	2 cut(s)	194, 372
AlwI	GGATC	1 cut(s)	208
Ama87I	CYCGRG	1 cut(s)	284
AoxI	GGCC	3 cut(s)	62, 627, 659
ApeKI	GCWGC	4 cut(s)	119, 311, 545, 668
ApoI	RAATTY	1 cut(s)	95
AspS9I	GGNCC	4 cut(s)	63, 598, 628, 660
AsuC2I	CCSGG	2 cut(s)	285, 286
AsuHPI	GGTGA	1 cut(s)	498
AsuII	TTCGAA	1 cut(s)	93
AvaI	CYCGRG	1 cut(s)	284
AvaII	GGWCC	1 cut(s)	598
BaeGI	GKGCMC	1 cut(s)	164
BanII	GRGCYC	1 cut(s)	372
BauI	CACGAG	1 cut(s)	642
BbsI	GAAGAC	1 cut(s)	305
Bbv12I	GWGCWC	2 cut(s)	194, 372
BbvCI	CCTCAGC	1 cut(s)	133
BbvI	GCAGC	4 cut(s)	106, 298, 532, 655
BccI	CCATC	2 cut(s)	565, 631
BceAI	ACGGC	4 cut(s)	89, 204, 314, 488
BciT130I	CCWGG	1 cut(s)	558
BclI	TGATCA	1 cut(s)	646
BcnI	CCSGG	2 cut(s)	285, 286
BfaI	CTAG	1 cut(s)	585
BfmI	CTRYAG	1 cut(s)	589
BisI	GCNGC	6 cut(s)	120, 123, 126, 312, 546, 669
BlsI	GCNGC	6 cut(s)	121, 124, 127, 313, 547, 670
Bme1390I	CCNGG	3 cut(s)	285, 286, 558
Bme18I	GGWCC	1 cut(s)	598
BmeT110I	CYCGRG	1 cut(s)	284
BmgT120I	GGNCC	4 cut(s)	63, 598, 628, 660
BmiI	GGNNCC	3 cut(s)	282, 600, 662
BmrFI	CCNGG	3 cut(s)	285, 286, 558
BmsI	GCATC	1 cut(s)	316
BpiI	GAAGAC	1 cut(s)	305
BpmI	CTGGAG	1 cut(s)	216
Bpu10I	CCTNAGC	2 cut(s)	133, 363
Bpu14I	TTCGAA	1 cut(s)	93
BpuMI	CCSGG	2 cut(s)	285, 286
BsaJI	CCNNGG	2 cut(s)	284, 557
Bsc4I	CCNNNNNNNGG	3 cut(s)	83, 171, 172
BseBI	CCWGG	1 cut(s)	558
BseDI	CCNNGG	2 cut(s)	284, 557
BseGI	GGATG	1 cut(s)	553
BseLI	CCNNNNNNNGG	3 cut(s)	83, 171, 172
BseMII	CTCAG	2 cut(s)	147, 377
BseRI	GAGGAG	1 cut(s)	247
BseSI	GKGCMC	1 cut(s)	164
BseXI	GCAGC	4 cut(s)	106, 298, 532, 655
BseYI	CCCAGC	1 cut(s)	532
BsgI	GTGCAG	1 cut(s)	459
BshFI	GGCC	3 cut(s)	64, 629, 661
BsiHKAI	GWGCWC	2 cut(s)	194, 372
BsiHKCI	CYCGRG	1 cut(s)	284
BsiSI	CCGG	2 cut(s)	116, 285
BslFI	GGGAC	2 cut(s)	189, 584
BslI	CCNNNNNNNGG	3 cut(s)	83, 171, 172
BsmFI	GGGAC	2 cut(s)	189, 584
BsnI	GGCC	3 cut(s)	64, 629, 661
BsoBI	CYCGRG	1 cut(s)	284
Bsp119I	TTCGAA	1 cut(s)	93
Bsp1286I	GDGCHC	3 cut(s)	164, 194, 372
Bsp143I	GATC	2 cut(s)	200, 646
BspACI	CCGC	5 cut(s)	102, 123, 126, 166, 270
BspANI	GGCC	3 cut(s)	64, 629, 661
BspCNI	CTCAG	2 cut(s)	146, 376
BspLI	GGNNCC	3 cut(s)	282, 600, 662
BspPI	GGATC	1 cut(s)	208
BspT104I	TTCGAA	1 cut(s)	93
BsrBI	CCGCTC	1 cut(s)	270
BssECI	CCNNGG	2 cut(s)	284, 557
BssMI	GATC	2 cut(s)	200, 646
BssSI	CACGAG	1 cut(s)	642
Bst2BI	CACGAG	1 cut(s)	642
Bst2UI	CCWGG	1 cut(s)	558
Bst4CI	ACNGT	1 cut(s)	144
BstBI	TTCGAA	1 cut(s)	93
BstC8I	GCNNGC	1 cut(s)	217
BstDEI	CTNAG	5 cut(s)	133, 350, 363, 656, 672
BstF5I	GGATG	1 cut(s)	553
BstKTI	GATC	2 cut(s)	203, 649
BstMBI	GATC	2 cut(s)	200, 646
BstMWI	GCNNNNNNNGC	4 cut(s)	125, 134, 481, 542
BstNI	CCWGG	1 cut(s)	558
BstSCI	CCNGG	3 cut(s)	283, 284, 556
BstSFI	CTRYAG	1 cut(s)	589
BstSLI	GKGCMC	1 cut(s)	164
BstV1I	GCAGC	4 cut(s)	106, 298, 532, 655
BstV2I	GAAGAC	1 cut(s)	305
BsuRI	GGCC	3 cut(s)	64, 629, 661
BtsCI	GGATG	1 cut(s)	553
Cac8I	GCNNGC	1 cut(s)	217
Cfr13I	GGNCC	4 cut(s)	63, 598, 628, 660
Cfr9I	CCCGGG	1 cut(s)	284
Csp6I	GTAC	1 cut(s)	539
CviQI	GTAC	1 cut(s)	539
DdeI	CTNAG	5 cut(s)	133, 350, 363, 656, 672
DpnI	GATC	2 cut(s)	202, 648
DpnII	GATC	2 cut(s)	200, 646
DraI	TTTAAA	1 cut(s)	414
EciI	GGCGGA	1 cut(s)	91
Ecl136II	GAGCTC	1 cut(s)	370
Eco24I	GRGCYC	1 cut(s)	372
Eco47I	GGWCC	1 cut(s)	598
Eco53kI	GAGCTC	1 cut(s)	370
Eco57I	CTGAAG	1 cut(s)	378
Eco88I	CYCGRG	1 cut(s)	284
EcoICRI	GAGCTC	1 cut(s)	370
EcoO109I	RGGNCCY	1 cut(s)	660
EcoRI	GAATTC	1 cut(s)	95
EcoRII	CCWGG	1 cut(s)	556
EcoT38I	GRGCYC	1 cut(s)	372
FaiI	YATR	7 cut(s)	317, 497, 550, 591, 605, 615, 617
FaqI	GGGAC	2 cut(s)	189, 584
FauI	CCCGC	1 cut(s)	173
FbaI	TGATCA	1 cut(s)	646
Fnu4HI	GCNGC	6 cut(s)	120, 123, 126, 312, 546, 669
FokI	GGATG	1 cut(s)	540
FriOI	GRGCYC	1 cut(s)	372
Fsp4HI	GCNGC	6 cut(s)	120, 123, 126, 312, 546, 669
FspBI	CTAG	1 cut(s)	585
GluI	GCNGC	6 cut(s)	120, 123, 126, 312, 546, 669
GsaI	CCCAGC	1 cut(s)	536
GsuI	CTGGAG	1 cut(s)	216
HaeIII	GGCC	3 cut(s)	64, 629, 661
HapII	CCGG	2 cut(s)	116, 285
HindIII	AAGCTT	1 cut(s)	213
HinfI	GANTC	1 cut(s)	185
HpaII	CCGG	2 cut(s)	116, 285
HphI	GGTGA	1 cut(s)	498
Hpy188III	TCNNGA	6 cut(s)	23, 195, 365, 454, 644, 650
Hpy99I	CGWCG	2 cut(s)	38, 110
HpyAV	CCTTC	2 cut(s)	87, 519
HpyCH4III	ACNGT	1 cut(s)	144
HpyCH4V	TGCA	2 cut(s)	440, 548
HpyF10VI	GCNNNNNNNGC	4 cut(s)	125, 134, 481, 542
HpyF3I	CTNAG	5 cut(s)	133, 350, 363, 656, 672
Ksp22I	TGATCA	1 cut(s)	646
Kzo9I	GATC	2 cut(s)	200, 646
LmnI	GCTCC	1 cut(s)	367
Lsp1109I	GCAGC	4 cut(s)	106, 298, 532, 655
LweI	GCATC	1 cut(s)	316
MaeI	CTAG	1 cut(s)	585
MaeIII	GTNAC	2 cut(s)	144, 390
MalI	GATC	2 cut(s)	202, 648
MbiI	CCGCTC	1 cut(s)	270
MboI	GATC	2 cut(s)	200, 646
MboII	GAAGA	3 cut(s)	310, 410, 436
MhlI	GDGCHC	3 cut(s)	164, 194, 372
MluCI	AATT	3 cut(s)	95, 227, 501
MmeI	TCCRAC	1 cut(s)	548
MnlI	CCTC	7 cut(s)	142, 148, 165, 191, 225, 228, 630
MseI	TTAA	2 cut(s)	405, 413
MspA1I	CMGCKG	1 cut(s)	545
MspI	CCGG	2 cut(s)	116, 285
MspR9I	CCNGG	3 cut(s)	285, 286, 558
MvaI	CCWGG	1 cut(s)	558
MwoI	GCNNNNNNNGC	4 cut(s)	125, 134, 481, 542
NciI	CCSGG	2 cut(s)	285, 286
NdeII	GATC	2 cut(s)	200, 646
NlaIV	GGNNCC	3 cut(s)	282, 600, 662
NmuCI	GTSAC	1 cut(s)	144
NspV	TTCGAA	1 cut(s)	93
PcsI	WCGNNNNNNNCGW	1 cut(s)	90
PfeI	GAWTC	1 cut(s)	185
PkrI	GCNGC	6 cut(s)	121, 124, 127, 313, 547, 670
Psp124BI	GAGCTC	1 cut(s)	372
Psp6I	CCWGG	1 cut(s)	556
PspFI	CCCAGC	1 cut(s)	532
PspGI	CCWGG	1 cut(s)	556
PspN4I	GGNNCC	3 cut(s)	282, 600, 662
PspPI	GGNCC	4 cut(s)	63, 598, 628, 660
PvuII	CAGCTG	1 cut(s)	545
RsaI	GTAC	1 cut(s)	540
RsaNI	GTAC	1 cut(s)	539
SacI	GAGCTC	1 cut(s)	372
SaqAI	TTAA	2 cut(s)	405, 413
SatI	GCNGC	6 cut(s)	120, 123, 126, 312, 546, 669
Sau3AI	GATC	2 cut(s)	200, 646
Sau96I	GGNCC	4 cut(s)	63, 598, 628, 660
ScrFI	CCNGG	3 cut(s)	285, 286, 558
SduI	GDGCHC	3 cut(s)	164, 194, 372
SfaNI	GCATC	1 cut(s)	316
SfcI	CTRYAG	1 cut(s)	589
SfuI	TTCGAA	1 cut(s)	93
SinI	GGWCC	1 cut(s)	598
SmaI	CCCGGG	1 cut(s)	286
Sse9I	AATT	3 cut(s)	95, 227, 501
SsiI	CCGC	5 cut(s)	102, 123, 126, 166, 270
SspMI	CTAG	1 cut(s)	585
SstI	GAGCTC	1 cut(s)	372
StyD4I	CCNGG	3 cut(s)	283, 284, 556
TaaI	ACNGT	1 cut(s)	144
TaqI	TCGA	3 cut(s)	33, 93, 108
TasI	AATT	3 cut(s)	95, 227, 501
TauI	GCSGC	2 cut(s)	125, 128
TfiI	GAWTC	1 cut(s)	185
Tru1I	TTAA	2 cut(s)	405, 413
Tru9I	TTAA	2 cut(s)	405, 413
TseFI	GTSAC	1 cut(s)	144
TseI	GCWGC	4 cut(s)	119, 311, 545, 668
Tsp45I	GTSAC	1 cut(s)	144
TspDTI	ATGAA	1 cut(s)	444
TspGWI	ACGGA	3 cut(s)	193, 197, 317
TspMI	CCCGGG	1 cut(s)	284
VpaK11BI	GGWCC	1 cut(s)	598
XapI	RAATTY	1 cut(s)	95
XmaI	CCCGGG	1 cut(s)	284
XspI	CTAG	1 cut(s)	585

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.