Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010373

Physical Location & Seq

Genomic Coordinates Reverse (-)

21075952 .. 21085603

Gene Structure 9652 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1216000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGGAGGAATGGATAGATTATCTTGATTACATCGACGAACCCCAACCCACCGATTTCCTCTGGCCCAACGGCACTCCTTTCGGCGATTTCGAATTCTCCGCCCTCGATGTTCCTGCTGCCGCCGTTTCCTCCGCCTCACAGTCACAGGAAAAAGAGTGCCCCCGCAAGAGGGGACGACCGGATTCGTGCTCTGGATCAGGGGCAAAAGCTTGCCGTGAGAAATTGAGGAGGGAGAGATTGAATGACAGGTTTGTGGAGTTGAACGGTGTTTTGGAGCCTGGGAGGCCGCCGAAGACGGACAAGGCTGCTATGCTTGATGATACCATAAAGGTTTTGAACCAGCTGAGAGCTGAAGCTCAGGAGCTCACAGAAACAAATCAAAAGTTACTTGAAGAATTTAAAAGTTTAAAGGCAGAGAAGAACGAATTTCGTGAAGAGAAACTCGTACTGAAAGCAGATAAAGAACGGCTGGAGCAGCAGTTGAAAGGTATGGCAATTTCACCTTCTGGGTTTGTTCCAACCCACCCAGCGGTACCAGCTGCATATCATCCTGGGGCAAACAAGATGGCAGTCTATCCTAGCTATAGTCTGGTCCCGATGTGGCACTATTTGCCTCCATCGGCCCGTGATACATCTCGTGATCATAAACTTAGGCCCCCCGCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009743 GO:0009746 GO:0009749 GO:0009889 GO:0009987 GO:0010029 GO:0010030 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019725 GO:0031323 GO:0031326 GO:0033500 GO:0034284 GO:0042221 GO:0042592 GO:0042593 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051239 GO:0051240 GO:0051252 GO:0051716 GO:0055082 GO:0060255 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0080090 GO:0097159 GO:0140110 GO:1900140 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

223

Amino Acids

25.02

Weight (kDa)

5.91

Isoelectric Point (pI)

55.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	68 - 116	4.1e-08	Helix-loop-helix DNA-binding domain
bHLH_IRO3	PF23177	69 - 133	2.8e-07	Iron-related transcription factor 3 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23210 AT3G23210 AT4G14410 AT4G14410 AT4G14410
fragaria_vesca	FvH4_4g03420
malus_domestica	MD13G1212400.v1.1 MD16G1216000.v1.1
prunus_persica	Prupe.1G037400_v2.0.a1
pyrus_communis	pycom13g18420 pycom16g18070
rosa_chinensis	RchiOBHm_Chr4g0392401
rosa_laevigata	RLG00000009814
rosa_multiflora	Rmu_sc0001800.1_g000017
rosa_roxburghii	Rroxscaffold_5G00337580
rosa_rugosa	Rorug03G0338600
rosa_samantha	Rh4AG042400 Rh4BG037300 Rh4CG045200 Rh4DG039700
rosa_wichuraiana	Rw4G003310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	532
AccB1I	GGYRCC	1 cut(s)	532
AciI	CCGC	7 cut(s)	99, 120, 132, 163, 287, 530, 660
AclWI	GGATC	1 cut(s)	202
AcsI	RAATTY	3 cut(s)	92, 395, 425
AcuI	CTGAAG	1 cut(s)	372
AfaI	GTAC	2 cut(s)	447, 534
AfiI	CCNNNNNNNGG	3 cut(s)	168, 169, 529
AgsI	TTSAA	5 cut(s)	241, 262, 337, 392, 484
AjnI	CCWGG	2 cut(s)	277, 550
AjuI	GAANNNNNNNTTGG	2 cut(s)	254, 286
AluBI	AGCT	7 cut(s)	209, 343, 350, 356, 364, 539, 582
AluI	AGCT	7 cut(s)	209, 343, 350, 356, 364, 539, 582
Alw21I	GWGCWC	2 cut(s)	191, 366
AlwI	GGATC	1 cut(s)	202
AoxI	GGCC	4 cut(s)	62, 284, 621, 653
ApeKI	GCWGC	5 cut(s)	116, 305, 475, 539, 662
ApoI	RAATTY	3 cut(s)	92, 395, 425
Asp718I	GGTACC	1 cut(s)	532
AspS9I	GGNCC	4 cut(s)	63, 592, 622, 654
AsuHPI	GGTGA	1 cut(s)	492
AsuII	TTCGAA	1 cut(s)	90
AvaII	GGWCC	1 cut(s)	592
BaeGI	GKGCMC	1 cut(s)	161
BanI	GGYRCC	1 cut(s)	532
BanII	GRGCYC	1 cut(s)	366
BauI	CACGAG	1 cut(s)	636
BbsI	GAAGAC	1 cut(s)	299
Bbv12I	GWGCWC	2 cut(s)	191, 366
BbvI	GCAGC	5 cut(s)	103, 292, 487, 526, 649
BccI	CCATC	2 cut(s)	559, 625
BceAI	ACGGC	4 cut(s)	85, 107, 198, 482
BcgI	CGANNNNNNTGC	4 cut(s)	61, 95, 95, 129
BciT130I	CCWGG	2 cut(s)	279, 552
BclI	TGATCA	1 cut(s)	640
BfaI	CTAG	1 cut(s)	579
BfmI	CTRYAG	1 cut(s)	583
BglI	GCCNNNNNGGC	1 cut(s)	283
BisI	GCNGC	7 cut(s)	117, 120, 287, 306, 476, 540, 663
BlsI	GCNGC	7 cut(s)	118, 121, 288, 307, 477, 541, 664
Bme1390I	CCNGG	2 cut(s)	279, 552
Bme18I	GGWCC	1 cut(s)	592
BmgT120I	GGNCC	4 cut(s)	63, 592, 622, 654
BmiI	GGNNCC	4 cut(s)	276, 534, 594, 656
BmrFI	CCNGG	2 cut(s)	279, 552
BpiI	GAAGAC	1 cut(s)	299
BpmI	CTGGAG	1 cut(s)	491
Bpu10I	CCTNAGC	1 cut(s)	357
Bpu14I	TTCGAA	1 cut(s)	90
BsaJI	CCNNGG	2 cut(s)	278, 551
BsaWI	WCCGGW	1 cut(s)	178
BsaXI	ACNNNNNCTCC	2 cut(s)	464, 494
Bsc4I	CCNNNNNNNGG	3 cut(s)	168, 169, 529
BseBI	CCWGG	2 cut(s)	279, 552
BseDI	CCNNGG	2 cut(s)	278, 551
BseGI	GGATG	1 cut(s)	547
BseLI	CCNNNNNNNGG	3 cut(s)	168, 169, 529
BseMII	CTCAG	2 cut(s)	335, 371
BseRI	GAGGAG	1 cut(s)	241
BseSI	GKGCMC	1 cut(s)	161
BseXI	GCAGC	5 cut(s)	103, 292, 487, 526, 649
BseYI	CCCAGC	1 cut(s)	526
Bsh1285I	CGRYCG	1 cut(s)	179
BshFI	GGCC	4 cut(s)	64, 286, 623, 655
BshNI	GGYRCC	1 cut(s)	532
BsiEI	CGRYCG	1 cut(s)	179
BsiHKAI	GWGCWC	2 cut(s)	191, 366
BsiSI	CCGG	1 cut(s)	179
BslFI	GGGAC	2 cut(s)	186, 578
BslI	CCNNNNNNNGG	3 cut(s)	168, 169, 529
BsmFI	GGGAC	2 cut(s)	186, 578
BsnI	GGCC	4 cut(s)	64, 286, 623, 655
Bsp119I	TTCGAA	1 cut(s)	90
Bsp1286I	GDGCHC	3 cut(s)	161, 191, 366
Bsp143I	GATC	2 cut(s)	194, 640
BspACI	CCGC	7 cut(s)	99, 120, 132, 163, 287, 530, 660
BspANI	GGCC	4 cut(s)	64, 286, 623, 655
BspCNI	CTCAG	2 cut(s)	336, 370
BspLI	GGNNCC	4 cut(s)	276, 534, 594, 656
BspPI	GGATC	1 cut(s)	202
BspT104I	TTCGAA	1 cut(s)	90
BspT107I	GGYRCC	1 cut(s)	532
BssECI	CCNNGG	2 cut(s)	278, 551
BssMI	GATC	2 cut(s)	194, 640
BssSI	CACGAG	1 cut(s)	636
Bst2BI	CACGAG	1 cut(s)	636
Bst2UI	CCWGG	2 cut(s)	279, 552
Bst4CI	ACNGT	2 cut(s)	141, 266
Bst6I	CTCTTC	1 cut(s)	429
BstAPI	GCANNNNNTGC	1 cut(s)	610
BstBI	TTCGAA	1 cut(s)	90
BstC8I	GCNNGC	1 cut(s)	211
BstDEI	CTNAG	4 cut(s)	344, 357, 650, 666
BstF5I	GGATG	1 cut(s)	547
BstKTI	GATC	2 cut(s)	197, 643
BstMBI	GATC	2 cut(s)	194, 640
BstMCI	CGRYCG	1 cut(s)	179
BstMWI	GCNNNNNNNGC	4 cut(s)	283, 475, 536, 610
BstNI	CCWGG	2 cut(s)	279, 552
BstSCI	CCNGG	2 cut(s)	277, 550
BstSFI	CTRYAG	1 cut(s)	583
BstSLI	GKGCMC	1 cut(s)	161
BstV1I	GCAGC	5 cut(s)	103, 292, 487, 526, 649
BstV2I	GAAGAC	1 cut(s)	299
BsuRI	GGCC	4 cut(s)	64, 286, 623, 655
BtsCI	GGATG	1 cut(s)	547
Cac8I	GCNNGC	1 cut(s)	211
Cfr13I	GGNCC	4 cut(s)	63, 592, 622, 654
Csp6I	GTAC	2 cut(s)	446, 533
CviQI	GTAC	2 cut(s)	446, 533
DdeI	CTNAG	4 cut(s)	344, 357, 650, 666
DpnI	GATC	2 cut(s)	196, 642
DpnII	GATC	2 cut(s)	194, 640
DraI	TTTAAA	2 cut(s)	400, 408
Eam1104I	CTCTTC	1 cut(s)	429
EarI	CTCTTC	1 cut(s)	429
EciI	GGCGGA	2 cut(s)	88, 121
Ecl136II	GAGCTC	1 cut(s)	364
Eco24I	GRGCYC	1 cut(s)	366
Eco47I	GGWCC	1 cut(s)	592
Eco53kI	GAGCTC	1 cut(s)	364
Eco57I	CTGAAG	1 cut(s)	372
EcoICRI	GAGCTC	1 cut(s)	364
EcoO109I	RGGNCCY	1 cut(s)	654
EcoRI	GAATTC	1 cut(s)	92
EcoRII	CCWGG	2 cut(s)	277, 550
EcoT38I	GRGCYC	1 cut(s)	366
FaiI	YATR	6 cut(s)	311, 326, 491, 544, 585, 645
FaqI	GGGAC	2 cut(s)	186, 578
FauI	CCCGC	2 cut(s)	170, 667
FbaI	TGATCA	1 cut(s)	640
Fnu4HI	GCNGC	7 cut(s)	117, 120, 287, 306, 476, 540, 663
FokI	GGATG	1 cut(s)	534
FriOI	GRGCYC	1 cut(s)	366
Fsp4HI	GCNGC	7 cut(s)	117, 120, 287, 306, 476, 540, 663
FspBI	CTAG	1 cut(s)	579
GluI	GCNGC	7 cut(s)	117, 120, 287, 306, 476, 540, 663
GsaI	CCCAGC	1 cut(s)	530
GsuI	CTGGAG	1 cut(s)	491
HaeIII	GGCC	4 cut(s)	64, 286, 623, 655
HapII	CCGG	1 cut(s)	179
HindIII	AAGCTT	1 cut(s)	207
HinfI	GANTC	1 cut(s)	182
HpaII	CCGG	1 cut(s)	179
HphI	GGTGA	1 cut(s)	492
Hpy188III	TCNNGA	6 cut(s)	23, 192, 359, 431, 595, 638
Hpy99I	CGWCG	1 cut(s)	38
HpyAV	CCTTC	1 cut(s)	513
HpyCH4III	ACNGT	2 cut(s)	141, 266
HpyCH4V	TGCA	1 cut(s)	542
HpyF10VI	GCNNNNNNNGC	4 cut(s)	283, 475, 536, 610
HpyF3I	CTNAG	4 cut(s)	344, 357, 650, 666
KpnI	GGTACC	1 cut(s)	536
Ksp22I	TGATCA	1 cut(s)	640
Kzo9I	GATC	2 cut(s)	194, 640
LmnI	GCTCC	3 cut(s)	274, 361, 472
Lsp1109I	GCAGC	5 cut(s)	103, 292, 487, 526, 649
MaeI	CTAG	1 cut(s)	579
MaeIII	GTNAC	2 cut(s)	141, 384
MalI	GATC	2 cut(s)	196, 642
MboI	GATC	2 cut(s)	194, 640
MboII	GAAGA	4 cut(s)	304, 404, 430, 446
MhlI	GDGCHC	3 cut(s)	161, 191, 366
MluCI	AATT	5 cut(s)	92, 221, 395, 425, 495
MmeI	TCCRAC	1 cut(s)	542
MnlI	CCTC	9 cut(s)	68, 113, 139, 145, 162, 219, 222, 276, 624
MseI	TTAA	2 cut(s)	399, 407
MspA1I	CMGCKG	4 cut(s)	343, 530, 539, 662
MspI	CCGG	1 cut(s)	179
MspR9I	CCNGG	2 cut(s)	279, 552
MvaI	CCWGG	2 cut(s)	279, 552
MwoI	GCNNNNNNNGC	4 cut(s)	283, 475, 536, 610
NdeII	GATC	2 cut(s)	194, 640
NlaIV	GGNNCC	4 cut(s)	276, 534, 594, 656
NmuCI	GTSAC	1 cut(s)	141
NspV	TTCGAA	1 cut(s)	90
PcsI	WCGNNNNNNNCGW	1 cut(s)	87
PfeI	GAWTC	1 cut(s)	182
PkrI	GCNGC	7 cut(s)	118, 121, 288, 307, 477, 541, 664
Psp124BI	GAGCTC	1 cut(s)	366
Psp6I	CCWGG	2 cut(s)	277, 550
PspFI	CCCAGC	1 cut(s)	526
PspGI	CCWGG	2 cut(s)	277, 550
PspN4I	GGNNCC	4 cut(s)	276, 534, 594, 656
PspPI	GGNCC	4 cut(s)	63, 592, 622, 654
PvuII	CAGCTG	2 cut(s)	343, 539
RsaI	GTAC	2 cut(s)	447, 534
RsaNI	GTAC	2 cut(s)	446, 533
SacI	GAGCTC	1 cut(s)	366
SaqAI	TTAA	2 cut(s)	399, 407
SatI	GCNGC	7 cut(s)	117, 120, 287, 306, 476, 540, 663
Sau3AI	GATC	2 cut(s)	194, 640
Sau96I	GGNCC	4 cut(s)	63, 592, 622, 654
ScrFI	CCNGG	2 cut(s)	279, 552
SduI	GDGCHC	3 cut(s)	161, 191, 366
SfcI	CTRYAG	1 cut(s)	583
SfuI	TTCGAA	1 cut(s)	90
SinI	GGWCC	1 cut(s)	592
Sse9I	AATT	5 cut(s)	92, 221, 395, 425, 495
SsiI	CCGC	7 cut(s)	99, 120, 132, 163, 287, 530, 660
SspMI	CTAG	1 cut(s)	579
SstI	GAGCTC	1 cut(s)	366
StyD4I	CCNGG	2 cut(s)	277, 550
TaaI	ACNGT	2 cut(s)	141, 266
TaqI	TCGA	3 cut(s)	33, 90, 105
TasI	AATT	5 cut(s)	92, 221, 395, 425, 495
TauI	GCSGC	2 cut(s)	122, 289
TfiI	GAWTC	1 cut(s)	182
Tru1I	TTAA	2 cut(s)	399, 407
Tru9I	TTAA	2 cut(s)	399, 407
TseFI	GTSAC	1 cut(s)	141
TseI	GCWGC	5 cut(s)	116, 305, 475, 539, 662
Tsp45I	GTSAC	1 cut(s)	141
TspGWI	ACGGA	1 cut(s)	311
VpaK11BI	GGWCC	1 cut(s)	592
XapI	RAATTY	3 cut(s)	92, 395, 425
XspI	CTAG	1 cut(s)	579

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.