Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006848

Physical Location & Seq

Genomic Coordinates Forward (+)

24345236 .. 24349901

Gene Structure 4666 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1239200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1962 bp

ATGATTACTGGCAAGGACATATACGATGTTCTAGCAGCCATAGTCCCCTTATACGTGGCTATGATCTTAGCCTACGGTTCCGTCCGGTGGTGGAAAATCTTCACGCCCGACCAATGCTCCGGCATAAACCGCTTCGTCGCAGTTTTTGCGGTCCCATTGCTCTCCTTCCACTTCATATCCTCCAACAACCCTTACGCCATGGACTACCAATTCATCCTCGCCGACTCCCTCCAAAAAGTCGTCATCCTTGTCGCCCTCTTCCTCTGGCAAGCCTTTTCCAAGCGCGGCAACCTAGAGTGGATGATCACGCTCTTCTCCCTCTCCACCCTCCCCAACACCCTCGTCATGGGCATCCCGCTCTTACGCGCCATGTACGGCGACTTCTCCGGGACTCTGATGGTTCAGATTGTCGTTTTGCAGAGCGTTGTCTGGTACACTCTCATGCTCTTCATGTTCGAGTACCGCGGCGCCAAACTGCTCATCTCCGAGCAGTTCCCGGAGACTGCAGCTTCGATCACGTCGTTCAGAGTTGACTCTGACGTCGTTTCCCTCAACGGTCGAGAACCACTCCAAACCGACGCGGAAATCGGGGATGACGGGAAACTTCACGTGGTGGTGAAAAGATCGAATGCGTCATCGATGGTTTCATCCTTTAAGTCACATGGCTTGAACTCGTTGACTTCAATGACCCCGCGAGCTTCGAATCTCACAGGAGTGGAGATTTACTCTGTCCAGTCCTCCCGAGAGCCGACCCCGCGGGCATCCAGCTTCAACCAGACAGATTTTTACGCCATGTTTAACGCCAGCAAGGCCCCGAGCCCCAAACATGGATACGCAAACAGTTTTCAAGGTGGATTTGGGGACGTTTATTCGGTGCAATCGTCAAAAGGGGTGACGCCGAGGACTTCGAATTTCGACGAAGAGGCAATGAAGATGAGTAATATGGGTAATAAGAAAAGGGGGGCGAGGAGCATGAGTGGTGAGATCTTCAATGGTGGTGCTGTCTCTTCCTACCCGCCTCCGAACCCCATGTTTTCAGGGTCTACGAGTGGTGGGCCGAAGAAGAAAGATAGTGGTAGCGGCGGCGGCGGCGGCGGCGGCGGTAGTGGTGGGGCAGCGCCTAATAAGGAGCTTCATATGTTTGTCTGGAGTTCAAGTGCATCGCCAGTTTCCGAAGGGAATCTTAGACATGCGGTTAATCGAGCTGCTTCCACTGACTTTGGGGCGACCGATCCTTCCAAGGCTGCAGGTCTTCATCAACATGAAGGTGCTGCTGCATCGAAAGGTATACACGAATTAATTGAGAATATGAGTCCTGGGAGGAAAATGAGTGGAGATAGGGAGCTTGAGATAGAAGAAGGATCGAATAAGTTTCCACCGAGTGTGTCACCGTACAGTAGTACTAGTTGCCAGAAGAAAGTGGATATTGAAGGTGGTGTAGCAAAGAAGCACCAAATGCCTCCTGCAAGTGTCATGACTAGGCTTATTCTCATCATGGTCTGGAGGAAGCTCATTAGAAACCCCAACACTTACTCCAGCCTTCTTGGCGTGGCATGGTCTCTCATATCATACAAGTGGAACATCAAAATGCCAACAATTGTTAGTGGATCCATATCAATATTATCTGATGCTGGATTGGGAATGGCTATGTTCAGTCTCGGATTATTCATGGCTTTGCAACCGAAGATCATAGCTTGTGGAAAATCTGTTGCAACATTTTCAATGGCTGTTAGGTTCCTAACCGGCCCAGCAGTGATTGCTGCAACTTCTATTGCTGTCGGTCTTCGTGGAGTTCTGTTGCATGTTGCAATAGTTCAGGCTGCTCTTCCTCAAGGCATAGTTCCTTTTGTATTTGCCAAGGAATATAATGTCCATGCAGACATCCTTAGTACCGCGGTTATTTTTGGAATGCTCATTGCCCTGCCCATAACAATACTCTACTATATCCTTCTCGGGCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001666 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005886 GO:0006810 GO:0006950 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009743 GO:0009746 GO:0009749 GO:0009914 GO:0009921 GO:0009925 GO:0009926 GO:0009958 GO:0009966 GO:0010033 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0015562 GO:0016020 GO:0016323 GO:0022857 GO:0023051 GO:0032991 GO:0034284 GO:0036293 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045178 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0065007 GO:0065008 GO:0070482 GO:0071944 GO:0080161 GO:0098590 GO:0098796 GO:0098797 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mem_trans

Protein Analysis

654

Amino Acids

70.22

Weight (kDa)

9.33

Isoelectric Point (pI)

45.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mem_trans	PF03547	10 - 648	1e-121	Membrane transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006848)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57090 AT5G57090
fragaria_vesca	FvH4_4g06870
malus_domestica	MD13G1239200.v1.1 MD16G1244500.v1.1
prunus_persica	Prupe.1G071700_v2.0.a1 Prupe.1G071800_v2.0.a1
pyrus_communis	pycom16g20590
rosa_chinensis	RchiOBHm_Chr4g0398751 RchiOBHm_Chr4g0398781
rosa_laevigata	RLG00000009323 RLG00000009324
rosa_multiflora	Rmu_sc0000458.1_g000007
rosa_roxburghii	Rroxscaffold_5G00343700 Rroxscaffold_5G00343710
rosa_rugosa	Rorug04G0011300 Rorug04G0011400
rosa_samantha	Rh4AG089200 Rh4AG089300 Rh4BG086600 Rh4CG097200 Rh4CG097400 Rh4DG080900 Rh4DG081100
rosa_wichuraiana	Rw4G007370 Rw4G007380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	539
AatII	GACGTC	1 cut(s)	543
Acc36I	ACCTGC	1 cut(s)	1238
AccB1I	GGYRCC	1 cut(s)	467
AccBSI	CCGCTC	1 cut(s)	358
AccI	GTMKAC	2 cut(s)	1043, 1288
AccII	CGCG	7 cut(s)	285, 366, 465, 581, 694, 757, 1895
AclWI	GGATC	4 cut(s)	1226, 1369, 1602, 1615
AcsI	RAATTY	1 cut(s)	910
AcvI	CACGTG	1 cut(s)	610
AcyI	GRCGYC	3 cut(s)	468, 540, 896
AdeI	CACNNNGTG	2 cut(s)	613, 1382
AfaI	GTAC	6 cut(s)	374, 434, 461, 1394, 1402, 1891
AfiI	CCNNNNNNNGG	3 cut(s)	87, 346, 827
AgsI	TTSAA	8 cut(s)	670, 684, 772, 848, 991, 1155, 1430, 1722
AhlI	ACTAGT	1 cut(s)	1403
AjiI	CACGTC	1 cut(s)	519
AjnI	CCWGG	1 cut(s)	1315
AleI	CACNNNNGTG	1 cut(s)	713
AluBI	AGCT	8 cut(s)	509, 698, 768, 1132, 1205, 1345, 1510, 1694
AluI	AGCT	8 cut(s)	509, 698, 768, 1132, 1205, 1345, 1510, 1694
Alw26I	GTCTC	4 cut(s)	494, 1009, 1563, 1661
AlwI	GGATC	4 cut(s)	1226, 1369, 1602, 1615
Ama87I	CYCGRG	3 cut(s)	741, 814, 1952
AoxI	GGCC	3 cut(s)	810, 1055, 1744
ApeKI	GCWGC	9 cut(s)	35, 506, 1115, 1205, 1244, 1271, 1274, 1760, 1820
ApoI	RAATTY	1 cut(s)	910
ArsI	GACNNNNNNTTYG	2 cut(s)	493, 525
AseI	ATTAAT	1 cut(s)	1298
Asp700I	GAANNNNTTC	1 cut(s)	98
AspLEI	GCGC	4 cut(s)	285, 368, 470, 1120
AspS9I	GGNCC	4 cut(s)	151, 811, 1055, 1745
AsuC2I	CCSGG	2 cut(s)	388, 497
AsuHPI	GGTGA	4 cut(s)	628, 904, 992, 1380
AsuII	TTCGAA	2 cut(s)	701, 908
AvaI	CYCGRG	3 cut(s)	741, 814, 1952
AvaII	GGWCC	1 cut(s)	151
BamHI	GGATCC	1 cut(s)	1607
BanI	GGYRCC	1 cut(s)	467
BanII	GRGCYC	2 cut(s)	821, 1959
BarI	GAAGNNNNNNTAC	2 cut(s)	365, 397
BbrPI	CACGTG	1 cut(s)	610
BbsI	GAAGAC	2 cut(s)	1244, 1775
BbvI	GCAGC	9 cut(s)	47, 518, 1127, 1192, 1231, 1258, 1261, 1747, 1807
BccI	CCATC	2 cut(s)	391, 634
BceAI	ACGGC	1 cut(s)	391
BciT130I	CCWGG	1 cut(s)	1317
BciVI	GTATCC	1 cut(s)	824
BclI	TGATCA	1 cut(s)	303
BcnI	CCSGG	2 cut(s)	388, 497
BcoDI	GTCTC	4 cut(s)	494, 1009, 1563, 1661
BcuI	ACTAGT	1 cut(s)	1403
BfaI	CTAG	5 cut(s)	32, 293, 1404, 1479, 1960
BfmI	CTRYAG	2 cut(s)	504, 1245
BfoI	RGCGCY	2 cut(s)	471, 1121
BfuAI	ACCTGC	1 cut(s)	1238
BfuI	GTATCC	1 cut(s)	824
BglI	GCCNNNNNGGC	2 cut(s)	809, 1545
BglII	AGATCT	1 cut(s)	984
BmcAI	AGTACT	1 cut(s)	1402
Bme1390I	CCNGG	3 cut(s)	388, 497, 1317
Bme18I	GGWCC	1 cut(s)	151
BmeT110I	CYCGRG	3 cut(s)	741, 814, 1952
BmgBI	CACGTC	1 cut(s)	519
BmgT120I	GGNCC	4 cut(s)	151, 811, 1055, 1745
BmiI	GGNNCC	6 cut(s)	79, 153, 469, 813, 1609, 1736
BmrFI	CCNGG	3 cut(s)	388, 497, 1317
BmsI	GCATC	5 cut(s)	360, 770, 1169, 1286, 1618
BpiI	GAAGAC	2 cut(s)	1244, 1775
BplI	GAGNNNNNCTC	4 cut(s)	552, 584, 710, 742
BpmI	CTGGAG	3 cut(s)	1168, 1519, 1522
Bpu14I	TTCGAA	2 cut(s)	701, 908
BpuEI	CTTGAG	2 cut(s)	1367, 1815
BpuMI	CCSGG	2 cut(s)	388, 497
Bsa29I	ATCGAT	1 cut(s)	638
BsaAI	YACGTR	2 cut(s)	55, 610
BsaBI	GATNNNNATC	1 cut(s)	1612
BsaHI	GRCGYC	3 cut(s)	468, 540, 896
BsaI	GGTCTC	1 cut(s)	1563
BsaJI	CCNNGG	8 cut(s)	198, 463, 755, 899, 1239, 1316, 1857, 1893
BsaWI	WCCGGW	1 cut(s)	84
BsaXI	ACNNNNNCTCC	8 cut(s)	101, 131, 961, 991, 1141, 1171, 1517, 1547
Bsc4I	CCNNNNNNNGG	3 cut(s)	87, 346, 827
Bse118I	RCCGGY	1 cut(s)	1742
Bse1I	ACTGG	3 cut(s)	13, 733, 1166
Bse3DI	GCAATG	3 cut(s)	155, 933, 1914
Bse8I	GATNNNNATC	1 cut(s)	1612
BseBI	CCWGG	1 cut(s)	1317
BseCI	ATCGAT	1 cut(s)	638
BseDI	CCNNGG	8 cut(s)	198, 463, 755, 899, 1239, 1316, 1857, 1893
BseGI	GGATG	8 cut(s)	213, 243, 306, 351, 598, 647, 761, 1881
BseJI	GATNNNNATC	1 cut(s)	1612
BseLI	CCNNNNNNNGG	3 cut(s)	87, 346, 827
BseMI	GCAATG	3 cut(s)	155, 933, 1914
BseNI	ACTGG	3 cut(s)	13, 733, 1166
BseRI	GAGGAG	1 cut(s)	982
BseXI	GCAGC	9 cut(s)	47, 518, 1127, 1192, 1231, 1258, 1261, 1747, 1807
BseYI	CCCAGC	1 cut(s)	1747
Bsh1236I	CGCG	7 cut(s)	285, 366, 465, 581, 694, 757, 1895
Bsh1285I	CGRYCG	2 cut(s)	559, 1230
BshFI	GGCC	3 cut(s)	812, 1057, 1746
BshNI	GGYRCC	1 cut(s)	467
BshVI	ATCGAT	1 cut(s)	638
BsiEI	CGRYCG	2 cut(s)	559, 1230
BsiHKCI	CYCGRG	3 cut(s)	741, 814, 1952
BsiSI	CCGG	5 cut(s)	85, 120, 387, 497, 1743
BslFI	GGGAC	4 cut(s)	29, 137, 403, 875
BslI	CCNNNNNNNGG	3 cut(s)	87, 346, 827
BsmAI	GTCTC	4 cut(s)	494, 1009, 1563, 1661
BsmFI	GGGAC	4 cut(s)	29, 137, 403, 875
BsmI	GAATGC	2 cut(s)	634, 1914
BsnI	GGCC	3 cut(s)	812, 1057, 1746
Bso31I	GGTCTC	1 cut(s)	1563
BsoBI	CYCGRG	3 cut(s)	741, 814, 1952
Bsp119I	TTCGAA	2 cut(s)	701, 908
Bsp1286I	GDGCHC	2 cut(s)	821, 1959
Bsp143I	GATC	9 cut(s)	63, 303, 513, 623, 984, 1231, 1361, 1607, 1686
Bsp19I	CCATGG	1 cut(s)	198
BspANI	GGCC	3 cut(s)	812, 1057, 1746
BspDI	ATCGAT	1 cut(s)	638
BspFNI	CGCG	7 cut(s)	285, 366, 465, 581, 694, 757, 1895
BspHI	TCATGA	1 cut(s)	1473
BspLI	GGNNCC	6 cut(s)	79, 153, 469, 813, 1609, 1736
BspMAI	CTGCAG	2 cut(s)	508, 1249
BspMI	ACCTGC	1 cut(s)	1238
BspPI	GGATC	4 cut(s)	1226, 1369, 1602, 1615
BspQI	GCTCTTC	3 cut(s)	317, 452, 1830
BspT104I	TTCGAA	2 cut(s)	701, 908
BspT107I	GGYRCC	1 cut(s)	467
BspTNI	GGTCTC	1 cut(s)	1563
BsrBI	CCGCTC	1 cut(s)	358
BsrDI	GCAATG	3 cut(s)	155, 933, 1914
BsrFI	RCCGGY	1 cut(s)	1742
BsrI	ACTGG	3 cut(s)	13, 733, 1166
BssAI	RCCGGY	1 cut(s)	1742
BssECI	CCNNGG	8 cut(s)	198, 463, 755, 899, 1239, 1316, 1857, 1893
BssMI	GATC	9 cut(s)	63, 303, 513, 623, 984, 1231, 1361, 1607, 1686
BssNAI	GTATAC	1 cut(s)	1289
BssNI	GRCGYC	3 cut(s)	468, 540, 896
BssT1I	CCWWGG	3 cut(s)	198, 1239, 1857
Bst1107I	GTATAC	1 cut(s)	1289
Bst2UI	CCWGG	1 cut(s)	1317
Bst4CI	ACNGT	5 cut(s)	77, 557, 842, 1392, 1397
Bst6I	CTCTTC	6 cut(s)	263, 317, 452, 915, 1012, 1830
BstACI	GRCGYC	3 cut(s)	468, 540, 896
BstAPI	GCANNNNNTGC	3 cut(s)	146, 1456, 1757
BstBAI	YACGTR	2 cut(s)	55, 610
BstBI	TTCGAA	2 cut(s)	701, 908
BstC8I	GCNNGC	4 cut(s)	270, 696, 759, 805
BstDEI	CTNAG	3 cut(s)	67, 1184, 1886
BstDSI	CCRYGG	4 cut(s)	198, 463, 755, 1893
BstF5I	GGATG	8 cut(s)	213, 243, 306, 351, 598, 647, 761, 1881
BstFNI	CGCG	7 cut(s)	285, 366, 465, 581, 694, 757, 1895
BstH2I	RGCGCY	2 cut(s)	471, 1121
BstHHI	GCGC	4 cut(s)	285, 368, 470, 1120
BstKTI	GATC	9 cut(s)	66, 306, 516, 626, 987, 1234, 1364, 1610, 1689
BstMAI	GTCTC	4 cut(s)	494, 1009, 1563, 1661
BstMBI	GATC	9 cut(s)	63, 303, 513, 623, 984, 1231, 1361, 1607, 1686
BstMCI	CGRYCG	2 cut(s)	559, 1230
BstNI	CCWGG	1 cut(s)	1317
BstNSI	RCATGY	2 cut(s)	1193, 1805
BstSCI	CCNGG	3 cut(s)	386, 495, 1315
BstSFI	CTRYAG	2 cut(s)	504, 1245
BstUI	CGCG	7 cut(s)	285, 366, 465, 581, 694, 757, 1895
BstV1I	GCAGC	9 cut(s)	47, 518, 1127, 1192, 1231, 1258, 1261, 1747, 1807
BstV2I	GAAGAC	2 cut(s)	1244, 1775
BstX2I	RGATCY	2 cut(s)	984, 1607
BstYI	RGATCY	2 cut(s)	984, 1607
BstZ17I	GTATAC	1 cut(s)	1289
Bsu15I	ATCGAT	1 cut(s)	638
BsuI	GTATCC	1 cut(s)	824
BsuRI	GGCC	3 cut(s)	812, 1057, 1746
BsuTUI	ATCGAT	1 cut(s)	638
BtgI	CCRYGG	4 cut(s)	198, 463, 755, 1893
BtgZI	GCGATG	1 cut(s)	1146
BtrI	CACGTC	1 cut(s)	519
BtsCI	GGATG	8 cut(s)	213, 243, 306, 351, 598, 647, 761, 1881
BtsI	GCAGTG	1 cut(s)	1758
BtsIMutI	CAGTG	2 cut(s)	1212, 1758
BveI	ACCTGC	1 cut(s)	1238
Cac8I	GCNNGC	4 cut(s)	270, 696, 759, 805
CciI	TCATGA	1 cut(s)	1473
CfoI	GCGC	4 cut(s)	285, 368, 470, 1120
Cfr10I	RCCGGY	1 cut(s)	1742
Cfr13I	GGNCC	4 cut(s)	151, 811, 1055, 1745
Cfr42I	CCGCGG	3 cut(s)	466, 758, 1896
ClaI	ATCGAT	1 cut(s)	638
CseI	GACGC	3 cut(s)	587, 621, 904
Csp6I	GTAC	6 cut(s)	373, 433, 460, 1393, 1401, 1890
CspCI	CAANNNNNGTGG	2 cut(s)	1201, 1236
CviQI	GTAC	6 cut(s)	373, 433, 460, 1393, 1401, 1890
DdeI	CTNAG	3 cut(s)	67, 1184, 1886
DinI	GGCGCC	1 cut(s)	469
DpnI	GATC	9 cut(s)	65, 305, 515, 625, 986, 1233, 1363, 1609, 1688
DpnII	GATC	9 cut(s)	63, 303, 513, 623, 984, 1231, 1361, 1607, 1686
DraIII	CACNNNGTG	2 cut(s)	613, 1382
DrdI	GACNNNNNNGTC	1 cut(s)	539
DseDI	GACNNNNNNGTC	1 cut(s)	539
Eam1104I	CTCTTC	6 cut(s)	263, 317, 452, 915, 1012, 1830
EarI	CTCTTC	6 cut(s)	263, 317, 452, 915, 1012, 1830
Eco130I	CCWWGG	3 cut(s)	198, 1239, 1857
Eco24I	GRGCYC	2 cut(s)	821, 1959
Eco31I	GGTCTC	1 cut(s)	1563
Eco47I	GGWCC	1 cut(s)	151
Eco72I	CACGTG	1 cut(s)	610
Eco88I	CYCGRG	3 cut(s)	741, 814, 1952
EcoO109I	RGGNCCY	1 cut(s)	811
EcoRII	CCWGG	1 cut(s)	1315
EcoT14I	CCWWGG	3 cut(s)	198, 1239, 1857
EcoT38I	GRGCYC	2 cut(s)	821, 1959
EgeI	GGCGCC	1 cut(s)	469
EheI	GGCGCC	1 cut(s)	469
ErhI	CCWWGG	3 cut(s)	198, 1239, 1857
FalI	AAGNNNNNCTT	2 cut(s)	1167, 1199
FaqI	GGGAC	4 cut(s)	29, 137, 403, 875
FauI	CCCGC	5 cut(s)	363, 699, 750, 762, 1023
FauNDI	CATATG	1 cut(s)	1137
FbaI	TGATCA	1 cut(s)	303
FblI	GTMKAC	2 cut(s)	1043, 1288
FokI	GGATG	8 cut(s)	200, 230, 313, 338, 605, 634, 748, 1868
FriOI	GRGCYC	2 cut(s)	821, 1959
FspBI	CTAG	5 cut(s)	32, 293, 1404, 1479, 1960
GlaI	GCGC	4 cut(s)	284, 367, 469, 1119
GsaI	CCCAGC	1 cut(s)	1751
GsuI	CTGGAG	3 cut(s)	1168, 1519, 1522
HaeII	RGCGCY	2 cut(s)	471, 1121
HaeIII	GGCC	3 cut(s)	812, 1057, 1746
HapII	CCGG	5 cut(s)	85, 120, 387, 497, 1743
HgaI	GACGC	3 cut(s)	587, 621, 904
HhaI	GCGC	4 cut(s)	285, 368, 470, 1120
Hin1I	GRCGYC	3 cut(s)	468, 540, 896
Hin6I	GCGC	4 cut(s)	283, 366, 468, 1118
HinP1I	GCGC	4 cut(s)	283, 366, 468, 1118
HincII	GTYRAC	2 cut(s)	532, 678
HindII	GTYRAC	2 cut(s)	532, 678
HinfI	GANTC	6 cut(s)	224, 391, 533, 703, 1180, 1312
HpaII	CCGG	5 cut(s)	85, 120, 387, 497, 1743
HphI	GGTGA	4 cut(s)	628, 904, 992, 1380
Hpy166II	GTNNAC	5 cut(s)	435, 532, 678, 1044, 1289
Hpy188I	TCNGA	9 cut(s)	396, 405, 487, 527, 538, 1023, 1174, 1627, 1661
Hpy188III	TCNNGA	5 cut(s)	560, 741, 1147, 1474, 1501
Hpy8I	GTNNAC	5 cut(s)	435, 532, 678, 1044, 1289
Hpy99I	CGWCG	5 cut(s)	140, 523, 545, 581, 920
HpyAV	CCTTC	8 cut(s)	175, 1169, 1245, 1259, 1352, 1424, 1550, 1958
HpyCH4III	ACNGT	5 cut(s)	77, 557, 842, 1392, 1397
HpyCH4IV	ACGT	5 cut(s)	54, 518, 540, 609, 864
HpyF3I	CTNAG	3 cut(s)	67, 1184, 1886
HpySE526I	ACGT	5 cut(s)	54, 518, 540, 609, 864
Hsp92I	GRCGYC	3 cut(s)	468, 540, 896
HspAI	GCGC	4 cut(s)	283, 366, 468, 1118
KasI	GGCGCC	1 cut(s)	467
Ksp22I	TGATCA	1 cut(s)	303
KspI	CCGCGG	3 cut(s)	466, 758, 1896
Kzo9I	GATC	9 cut(s)	63, 303, 513, 623, 984, 1231, 1361, 1607, 1686
LguI	GCTCTTC	3 cut(s)	317, 452, 1830
LmnI	GCTCC	4 cut(s)	122, 969, 1129, 1342
Lsp1109I	GCAGC	9 cut(s)	47, 518, 1127, 1192, 1231, 1258, 1261, 1747, 1807
LweI	GCATC	5 cut(s)	360, 770, 1169, 1286, 1618
MaeI	CTAG	5 cut(s)	32, 293, 1404, 1479, 1960
MaeII	ACGT	5 cut(s)	54, 518, 540, 609, 864
MaeIII	GTNAC	3 cut(s)	657, 892, 1386
MalI	GATC	9 cut(s)	65, 305, 515, 625, 986, 1233, 1363, 1609, 1688
MbiI	CCGCTC	1 cut(s)	358
MboI	GATC	9 cut(s)	63, 303, 513, 623, 984, 1231, 1361, 1607, 1686
MfeI	CAATTG	1 cut(s)	1596
MflI	RGATCY	2 cut(s)	984, 1607
MhlI	GDGCHC	2 cut(s)	821, 1959
MluCI	AATT	5 cut(s)	209, 910, 1295, 1299, 1596
Mly113I	GGCGCC	1 cut(s)	468
MlyI	GAGTC	4 cut(s)	218, 385, 527, 1321
MmeI	TCCRAC	1 cut(s)	207
MroXI	GAANNNNTTC	1 cut(s)	98
MseI	TTAA	4 cut(s)	654, 798, 1197, 1298
MslI	CAYNNNNRTG	6 cut(s)	440, 713, 1260, 1266, 1573, 1586
MspA1I	CMGCKG	3 cut(s)	465, 757, 1895
MspI	CCGG	5 cut(s)	85, 120, 387, 497, 1743
MspR9I	CCNGG	3 cut(s)	388, 497, 1317
MunI	CAATTG	1 cut(s)	1596
Mva1269I	GAATGC	2 cut(s)	634, 1914
MvaI	CCWGG	1 cut(s)	1317
MvnI	CGCG	7 cut(s)	285, 366, 465, 581, 694, 757, 1895
NarI	GGCGCC	1 cut(s)	468
NciI	CCSGG	2 cut(s)	388, 497
NcoI	CCATGG	1 cut(s)	198
NdeI	CATATG	1 cut(s)	1137
NdeII	GATC	9 cut(s)	63, 303, 513, 623, 984, 1231, 1361, 1607, 1686
NlaIV	GGNNCC	6 cut(s)	79, 153, 469, 813, 1609, 1736
NmeAIII	GCCGAG	1 cut(s)	924
NmuCI	GTSAC	3 cut(s)	657, 892, 1386
NspI	RCATGY	2 cut(s)	1193, 1805
NspV	TTCGAA	2 cut(s)	701, 908
OliI	CACNNNNGTG	1 cut(s)	713
PagI	TCATGA	1 cut(s)	1473
PciSI	GCTCTTC	3 cut(s)	317, 452, 1830
PcsI	WCGNNNNNNNCGW	2 cut(s)	518, 878
PctI	GAATGC	2 cut(s)	634, 1914
PdmI	GAANNNNTTC	1 cut(s)	98
PfeI	GAWTC	2 cut(s)	703, 1180
PfoI	TCCNGGA	2 cut(s)	386, 495
PleI	GAGTC	4 cut(s)	218, 385, 527, 1320
PluTI	GGCGCC	1 cut(s)	471
PmaCI	CACGTG	1 cut(s)	610
PmlI	CACGTG	1 cut(s)	610
PpsI	GAGTC	4 cut(s)	218, 385, 527, 1320
Ppu21I	YACGTR	2 cut(s)	55, 610
PshBI	ATTAAT	1 cut(s)	1298
Psp6I	CCWGG	1 cut(s)	1315
PspCI	CACGTG	1 cut(s)	610
PspFI	CCCAGC	1 cut(s)	1747
PspGI	CCWGG	1 cut(s)	1315
PspN4I	GGNNCC	6 cut(s)	79, 153, 469, 813, 1609, 1736
PspPI	GGNCC	4 cut(s)	151, 811, 1055, 1745
PstI	CTGCAG	2 cut(s)	508, 1249
PsuI	RGATCY	2 cut(s)	984, 1607
RsaI	GTAC	6 cut(s)	374, 434, 461, 1394, 1402, 1891
RsaNI	GTAC	6 cut(s)	373, 433, 460, 1393, 1401, 1890
RseI	CAYNNNNRTG	6 cut(s)	440, 713, 1260, 1266, 1573, 1586
SacII	CCGCGG	3 cut(s)	466, 758, 1896
SapI	GCTCTTC	3 cut(s)	317, 452, 1830
SaqAI	TTAA	4 cut(s)	654, 798, 1197, 1298
Sau3AI	GATC	9 cut(s)	63, 303, 513, 623, 984, 1231, 1361, 1607, 1686
Sau96I	GGNCC	4 cut(s)	151, 811, 1055, 1745
ScaI	AGTACT	1 cut(s)	1402
SchI	GAGTC	4 cut(s)	218, 385, 527, 1321
ScrFI	CCNGG	3 cut(s)	388, 497, 1317
SduI	GDGCHC	2 cut(s)	821, 1959
SfaNI	GCATC	5 cut(s)	360, 770, 1169, 1286, 1618
SfcI	CTRYAG	2 cut(s)	504, 1245
SfoI	GGCGCC	1 cut(s)	469
Sfr303I	CCGCGG	3 cut(s)	466, 758, 1896
SfuI	TTCGAA	2 cut(s)	701, 908
SgrBI	CCGCGG	3 cut(s)	466, 758, 1896
SinI	GGWCC	1 cut(s)	151
SmiMI	CAYNNNNRTG	6 cut(s)	440, 713, 1260, 1266, 1573, 1586
SmlI	CTYRAG	2 cut(s)	1346, 1830
SmoI	CTYRAG	2 cut(s)	1346, 1830
SpeI	ACTAGT	1 cut(s)	1403
Sse9I	AATT	5 cut(s)	209, 910, 1295, 1299, 1596
SspDI	GGCGCC	1 cut(s)	467
SspI	AATATT	1 cut(s)	1620
SspMI	CTAG	5 cut(s)	32, 293, 1404, 1479, 1960
StyD4I	CCNGG	3 cut(s)	386, 495, 1315
StyI	CCWWGG	3 cut(s)	198, 1239, 1857
TaaI	ACNGT	5 cut(s)	77, 557, 842, 1392, 1397
TaiI	ACGT	5 cut(s)	57, 521, 543, 612, 867
TaqII	GACCGA	2 cut(s)	1244, 1769
TasI	AATT	5 cut(s)	209, 910, 1295, 1299, 1596
TatI	WGTACW	1 cut(s)	1400
TauI	GCSGC	9 cut(s)	288, 468, 1083, 1086, 1089, 1092, 1095, 1098, 1101
TfiI	GAWTC	2 cut(s)	703, 1180
Tru1I	TTAA	4 cut(s)	654, 798, 1197, 1298
Tru9I	TTAA	4 cut(s)	654, 798, 1197, 1298
TscAI	CASTG	2 cut(s)	1219, 1758
TseFI	GTSAC	3 cut(s)	657, 892, 1386
TseI	GCWGC	9 cut(s)	35, 506, 1115, 1205, 1244, 1271, 1274, 1760, 1820
Tsp45I	GTSAC	3 cut(s)	657, 892, 1386
TspDTI	ATGAA	9 cut(s)	163, 202, 439, 636, 944, 1124, 1244, 1278, 1657
TspGWI	ACGGA	1 cut(s)	70
TspRI	CASTG	2 cut(s)	1219, 1758
VpaK11BI	GGWCC	1 cut(s)	151
VspI	ATTAAT	1 cut(s)	1298
XapI	RAATTY	1 cut(s)	910
XceI	RCATGY	2 cut(s)	1193, 1805
XmiI	GTMKAC	2 cut(s)	1043, 1288
XmnI	GAANNNNTTC	1 cut(s)	98
XspI	CTAG	5 cut(s)	32, 293, 1404, 1479, 1960
ZraI	GACGTC	1 cut(s)	541
ZrmI	AGTACT	1 cut(s)	1402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.