Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0006848

Physical Location & Seq

Genomic Coordinates Forward (+)

15580480 .. 15583287

Gene Structure 2808 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G007380.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGATCAGCGGAAAGGACATTTACGAGGTCCTTGCAAGTATTGTGCCGCTTTACGTGGCCATGATCTTGGCCTACGGCTCGGTCCGGTGGTGGAAGATCTTCACCCCGGACCAGTGCTCCGGCATCAACCGCTTCGTGGCGGTTTTTGCTGTTCCTTTGCTTTCATTTCACTTCATTTCTTCCAACGATCCCTACTCCATGGACTACCAGTTCATCGCGGCGGACTCGTTGCAGAAAGTGGTGATTCTTGTGGCTCTATTCCTCTGGCAGACCTTCTCCAAGCGTGGCAACCTCGAGTGGATGATCACGCTCTTCTCCCTCTCCTCTCTCCCCAACACTCTCGTCATGGGGATTCCTCTCTTGAAAGCCATGTACGGCGACTTTTCGGGCTCTCTCATGGTTCAAATCGTGGTGCTGCAGAGCGTCATCTGGTACACTCTCATGCTCTTCATGTTCGAGTACAGAGGAGCCAAACTCTTGATCTCAGAGCAGTTCCCGGAAACCGCCGGGTCCATTACCTCATTTCGAGTGGGCTCCGACGTCGTTTCGCTTAACGGACGCGAGCCTTTGCAGACCGATGCCGAGATCGGAGAGGATGGAAAGTTACACGTGGTGGTGAGAAGATCGGCGGCGTCGTCGATGGTGTCGTCGTTCAAGTCGCACGGGTTGAACTCTTTGAATTCCGGCATGACTCCTCGGGCTTCGAACCTGACTGGCGTTGAGATTTACTCGGTTCAGTCGTCTCGTGAGCCGACACCGAGGGCGTCCAGCTTCAACCAGACTGACTTCTACGCCATGTTCAATGCCAGCAAGGCGCCGAGCCCTAAACACGGCTACACCAACAGCTTCCAGGGCGGGTTTGGCGATGTTTACTCGGTCCAGTCGTCGAAAGGCGTGACCCCGAGGACTTCGAATTTTGATGAGGAGATGATGAAGATAAGCAACAAGAAGAGAGGGGCCAGGAGCATGAGCGGTGAGCTGTTCAATGCCGGGACCGTGTCCTCGTATCCGCCTCCGAATCCCATGTTTTCGGGGTCTACTAGTGGCGGACTGAAAAAGAAGGAAAGCGGCGGCGCCGGTGGTGGTAGTGGTGGTGCAGTGCCTAATAAGGAGCTTCATATGTTTGTTTGGAGTTCGAGTGCTTCGCCGGTTTCCGAGGGGAATCTGAAACATGCAGTTAACCGAGCTGCCTCTACTGATTTTGGAGGCATTGATCCTTCCAAGGCTGCTCAACATGATCATCCAATTGCTGGTTCAAAAGGGATGCATGAATTGATTCAGAATATGAGTCCTGGTAGGAAAATGCGTGGAGATGGGGAGCTTGAAATAGAAGAAGGAACAAAGTTTCCGGCAAGTGCATCTCCATACAGTAGCTGCCAGAAGAAGATGGACATGGAAGACGGAGGTGTGGCAAAGAAGCAACAAATGCCTCCTGCAAGTGTCATGACTAGGCTTATACTCATCATGGTGTGGAGGAAGCTCATTAGAAATCCCAACACCTACTCCAGTCTCCTTGGTGTCGCCTGGTCTCTCGTATCATACAAGTGGCACATCAAAATGCCCACAATTATAAGTGGATCCATATCAATATTATCTGACGCTGGCTTGGGGATGGCTATGTTCAGTCTAGGTTTATTCATGGCTTTACAACCAAAGATCATAGCTTGTGGAAAATCAGTAGCAACATTTGCAATGGCTGTCAGGTTCTTGACAGGCCCAGCAGTTATTGCTGCATGCTCCATAGCTGTTGGTCTTCGTGGAGTTCTTTTGCATGTTGCAATAGTTCAGGCTGCTCTTCCTCAAGGGATTGTTCCTTTTGTGTTTGCCAAAGAATACAATGTGCACGCAGACATACTTAGCACTGCGGTCATTTTTGGAATGCTGATTGCCTTGCCTATAACAATACTCTACTATATTCTTCTCGGGCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001666 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005886 GO:0006810 GO:0006950 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009743 GO:0009746 GO:0009749 GO:0009914 GO:0009921 GO:0009925 GO:0009926 GO:0009958 GO:0009966 GO:0010033 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0015562 GO:0016020 GO:0016323 GO:0022857 GO:0023051 GO:0032991 GO:0034284 GO:0036293 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045178 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0065007 GO:0065008 GO:0070482 GO:0071944 GO:0080161 GO:0098590 GO:0098796 GO:0098797 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mem_trans

Protein Analysis

643

Amino Acids

69.49

Weight (kDa)

9.28

Isoelectric Point (pI)

42.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mem_trans	PF03547	10 - 638	2.7e-120	Membrane transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0006848)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57090 AT5G57090
fragaria_vesca	FvH4_4g06870
malus_domestica	MD13G1239200.v1.1 MD16G1244500.v1.1
prunus_persica	Prupe.1G071700_v2.0.a1 Prupe.1G071800_v2.0.a1
pyrus_communis	pycom16g20590
rosa_chinensis	RchiOBHm_Chr4g0398751 RchiOBHm_Chr4g0398781
rosa_laevigata	RLG00000009323 RLG00000009324
rosa_multiflora	Rmu_sc0000458.1_g000007
rosa_roxburghii	Rroxscaffold_5G00343700 Rroxscaffold_5G00343710
rosa_rugosa	Rorug04G0011300 Rorug04G0011400
rosa_samantha	Rh4AG089200 Rh4AG089300 Rh4BG086600 Rh4CG097200 Rh4CG097400 Rh4DG080900 Rh4DG081100
rosa_wichuraiana	Rw4G007370 Rw4G007380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1571
AatII	GACGTC	1 cut(s)	543
AccB1I	GGYRCC	2 cut(s)	814, 1073
AccB7I	CCANNNNNTGG	1 cut(s)	1250
AccBSI	CCGCTC	1 cut(s)	972
AccI	GTMKAC	1 cut(s)	1037
AccII	CGCG	2 cut(s)	218, 561
AclWI	GGATC	4 cut(s)	182, 1208, 1572, 1585
AcoI	YGGCCR	1 cut(s)	57
AcsI	RAATTY	2 cut(s)	679, 913
AcvI	CACGTG	1 cut(s)	610
AcyI	GRCGYC	5 cut(s)	540, 632, 764, 815, 1074
AdeI	CACNNNGTG	1 cut(s)	613
AfaI	GTAC	3 cut(s)	374, 434, 461
AfiI	CCNNNNNNNGG	3 cut(s)	136, 830, 1250
AflIII	ACRYGT	1 cut(s)	607
AhlI	ACTAGT	1 cut(s)	1040
AjnI	CCWGG	4 cut(s)	849, 959, 1291, 1523
AjuI	GAANNNNNNNTTGG	2 cut(s)	1237, 1269
AloI	GAACNNNNNNTCC	4 cut(s)	194, 226, 1330, 1362
Alw21I	GWGCWC	2 cut(s)	119, 1845
Alw26I	GTCTC	3 cut(s)	747, 1514, 1533
Alw44I	GTGCAC	1 cut(s)	1841
AlwI	GGATC	4 cut(s)	182, 1208, 1572, 1585
AlwNI	CAGNNNCTG	1 cut(s)	1374
Ama87I	CYCGRG	4 cut(s)	293, 696, 901, 1922
AoxI	GGCC	4 cut(s)	57, 69, 957, 1714
ApaLI	GTGCAC	1 cut(s)	1841
ApeKI	GCWGC	6 cut(s)	415, 1187, 1226, 1374, 1730, 1790
ApoI	RAATTY	2 cut(s)	679, 913
Asp700I	GAANNNNTTC	2 cut(s)	98, 1275
AspLEI	GCGC	2 cut(s)	817, 1076
AspS9I	GGNCC	8 cut(s)	28, 82, 109, 510, 877, 957, 993, 1715
AsuC2I	CCSGG	4 cut(s)	107, 497, 508, 991
AsuHPI	GGTGA	4 cut(s)	94, 253, 628, 986
AsuII	TTCGAA	2 cut(s)	704, 911
AvaI	CYCGRG	4 cut(s)	293, 696, 901, 1922
AvaII	GGWCC	6 cut(s)	28, 82, 109, 510, 877, 993
BaeGI	GKGCMC	1 cut(s)	1845
BalI	TGGCCA	1 cut(s)	59
BamHI	GGATCC	1 cut(s)	1577
BanI	GGYRCC	2 cut(s)	814, 1073
BanII	GRGCYC	4 cut(s)	392, 536, 824, 1929
BauI	CACGAG	1 cut(s)	744
BbrPI	CACGTG	1 cut(s)	610
BbsI	GAAGAC	2 cut(s)	1404, 1745
Bbv12I	GWGCWC	2 cut(s)	119, 1845
BbvI	GCAGC	6 cut(s)	402, 1174, 1213, 1361, 1717, 1777
BccI	CCATC	5 cut(s)	590, 634, 1307, 1381, 1606
BceAI	ACGGC	3 cut(s)	91, 391, 847
BcgI	CGANNNNNNTGC	2 cut(s)	14, 48
BciT130I	CCWGG	4 cut(s)	851, 961, 1293, 1525
BciVI	GTATCC	1 cut(s)	1017
BclI	TGATCA	3 cut(s)	3, 303, 1237
BcnI	CCSGG	4 cut(s)	107, 497, 508, 991
BcoDI	GTCTC	3 cut(s)	747, 1514, 1533
BcuI	ACTAGT	1 cut(s)	1040
BfaI	CTAG	4 cut(s)	1041, 1449, 1628, 1930
BfmI	CTRYAG	1 cut(s)	416
BfoI	RGCGCY	2 cut(s)	818, 1077
BfuI	GTATCC	1 cut(s)	1017
BglI	GCCNNNNNGGC	1 cut(s)	812
BglII	AGATCT	1 cut(s)	96
Bme1390I	CCNGG	8 cut(s)	107, 497, 508, 851, 961, 991, 1293, 1525
Bme18I	GGWCC	6 cut(s)	28, 82, 109, 510, 877, 993
BmeT110I	CYCGRG	4 cut(s)	293, 696, 901, 1922
BmgT120I	GGNCC	8 cut(s)	28, 82, 109, 510, 877, 957, 993, 1715
BmiI	GGNNCC	8 cut(s)	469, 511, 535, 816, 958, 994, 1075, 1579
BmrFI	CCNGG	8 cut(s)	107, 497, 508, 851, 961, 991, 1293, 1525
BmsI	GCATC	4 cut(s)	132, 568, 1254, 1367
BpiI	GAAGAC	2 cut(s)	1404, 1745
BplI	GAGNNNNNCTC	4 cut(s)	459, 491, 713, 745
BpmI	CTGGAG	1 cut(s)	1489
Bpu14I	TTCGAA	2 cut(s)	704, 911
BpuEI	CTTGAG	1 cut(s)	1785
BpuMI	CCSGG	4 cut(s)	107, 497, 508, 991
BsaAI	YACGTR	2 cut(s)	55, 610
BsaBI	GATNNNNATC	1 cut(s)	1582
BsaHI	GRCGYC	5 cut(s)	540, 632, 764, 815, 1074
BsaI	GGTCTC	1 cut(s)	1533
BsaJI	CCNNGG	9 cut(s)	105, 198, 695, 758, 850, 902, 1155, 1221, 1513
BsaWI	WCCGGW	1 cut(s)	84
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 1123, 1153, 1487, 1517
Bsc4I	CCNNNNNNNGG	3 cut(s)	136, 830, 1250
Bse118I	RCCGGY	2 cut(s)	1076, 1147
Bse1I	ACTGG	5 cut(s)	112, 208, 718, 880, 1506
Bse3DI	GCAATG	1 cut(s)	1698
Bse8I	GATNNNNATC	1 cut(s)	1582
BseBI	CCWGG	4 cut(s)	851, 961, 1293, 1525
BseDI	CCNNGG	9 cut(s)	105, 198, 695, 758, 850, 902, 1155, 1221, 1513
BseGI	GGATG	5 cut(s)	306, 601, 1240, 1269, 1617
BseJI	GATNNNNATC	1 cut(s)	1582
BseLI	CCNNNNNNNGG	3 cut(s)	136, 830, 1250
BseMI	GCAATG	1 cut(s)	1698
BseMII	CTCAG	1 cut(s)	498
BseNI	ACTGG	5 cut(s)	112, 208, 718, 880, 1506
BseRI	GAGGAG	4 cut(s)	313, 480, 684, 938
BseSI	GKGCMC	1 cut(s)	1845
BseXI	GCAGC	6 cut(s)	402, 1174, 1213, 1361, 1717, 1777
BseYI	CCCAGC	1 cut(s)	1717
BsgI	GTGCAG	1 cut(s)	1116
Bsh1236I	CGCG	2 cut(s)	218, 561
BshFI	GGCC	4 cut(s)	59, 71, 959, 1716
BshNI	GGYRCC	2 cut(s)	814, 1073
BsiHKAI	GWGCWC	2 cut(s)	119, 1845
BsiHKCI	CYCGRG	4 cut(s)	293, 696, 901, 1922
BslFI	GGGAC	1 cut(s)	1006
BslI	CCNNNNNNNGG	3 cut(s)	136, 830, 1250
BsmAI	GTCTC	3 cut(s)	747, 1514, 1533
BsmBI	CGTCTC	1 cut(s)	747
BsmFI	GGGAC	1 cut(s)	1006
BsmI	GAATGC	1 cut(s)	1884
BsnI	GGCC	4 cut(s)	59, 71, 959, 1716
Bso31I	GGTCTC	1 cut(s)	1533
BsoBI	CYCGRG	4 cut(s)	293, 696, 901, 1922
Bsp119I	TTCGAA	2 cut(s)	704, 911
Bsp1286I	GDGCHC	6 cut(s)	119, 392, 536, 824, 1845, 1929
Bsp19I	CCATGG	1 cut(s)	198
BspANI	GGCC	4 cut(s)	59, 71, 959, 1716
BspCNI	CTCAG	1 cut(s)	497
BspFNI	CGCG	2 cut(s)	218, 561
BspHI	TCATGA	1 cut(s)	1443
BspLI	GGNNCC	8 cut(s)	469, 511, 535, 816, 958, 994, 1075, 1579
BspMAI	CTGCAG	1 cut(s)	420
BspPI	GGATC	4 cut(s)	182, 1208, 1572, 1585
BspQI	GCTCTTC	3 cut(s)	317, 452, 1800
BspT104I	TTCGAA	2 cut(s)	704, 911
BspT107I	GGYRCC	2 cut(s)	814, 1073
BspTNI	GGTCTC	1 cut(s)	1533
BsrBI	CCGCTC	1 cut(s)	972
BsrDI	GCAATG	1 cut(s)	1698
BsrFI	RCCGGY	2 cut(s)	1076, 1147
BsrI	ACTGG	5 cut(s)	112, 208, 718, 880, 1506
BssAI	RCCGGY	2 cut(s)	1076, 1147
BssECI	CCNNGG	9 cut(s)	105, 198, 695, 758, 850, 902, 1155, 1221, 1513
BssNI	GRCGYC	5 cut(s)	540, 632, 764, 815, 1074
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	3 cut(s)	198, 1221, 1513
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	4 cut(s)	851, 961, 1293, 1525
Bst4CI	ACNGT	2 cut(s)	997, 1370
Bst6I	CTCTTC	4 cut(s)	317, 452, 944, 1800
BstACI	GRCGYC	5 cut(s)	540, 632, 764, 815, 1074
BstAPI	GCANNNNNTGC	3 cut(s)	1426, 1688, 1727
BstBAI	YACGTR	2 cut(s)	55, 610
BstBI	TTCGAA	2 cut(s)	704, 911
BstC8I	GCNNGC	5 cut(s)	563, 808, 1603, 1735, 1845
BstDEI	CTNAG	2 cut(s)	484, 1856
BstDSI	CCRYGG	1 cut(s)	198
BstF5I	GGATG	5 cut(s)	306, 601, 1240, 1269, 1617
BstFNI	CGCG	2 cut(s)	218, 561
BstH2I	RGCGCY	2 cut(s)	818, 1077
BstHHI	GCGC	2 cut(s)	817, 1076
BstMAI	GTCTC	3 cut(s)	747, 1514, 1533
BstMWI	GCNNNNNNNGC	8 cut(s)	129, 146, 812, 852, 861, 1426, 1688, 1727
BstNI	CCWGG	4 cut(s)	851, 961, 1293, 1525
BstNSI	RCATGY	3 cut(s)	1175, 1737, 1775
BstSCI	CCNGG	8 cut(s)	105, 495, 506, 849, 959, 989, 1291, 1523
BstSFI	CTRYAG	1 cut(s)	416
BstSLI	GKGCMC	1 cut(s)	1845
BstUI	CGCG	2 cut(s)	218, 561
BstV1I	GCAGC	6 cut(s)	402, 1174, 1213, 1361, 1717, 1777
BstV2I	GAAGAC	2 cut(s)	1404, 1745
BstX2I	RGATCY	2 cut(s)	96, 1577
BstXI	CCANNNNNNTGG	1 cut(s)	67
BstYI	RGATCY	2 cut(s)	96, 1577
BsuI	GTATCC	1 cut(s)	1017
BsuRI	GGCC	4 cut(s)	59, 71, 959, 1716
BtgI	CCRYGG	1 cut(s)	198
BtgZI	GCGATG	2 cut(s)	199, 879
BtsCI	GGATG	5 cut(s)	306, 601, 1240, 1269, 1617
BtsI	GCAGTG	2 cut(s)	1104, 1860
BtsIMutI	CAGTG	3 cut(s)	119, 1104, 1860
Cac8I	GCNNGC	5 cut(s)	563, 808, 1603, 1735, 1845
CaiI	CAGNNNCTG	1 cut(s)	1374
CciI	TCATGA	1 cut(s)	1443
CfoI	GCGC	2 cut(s)	817, 1076
Cfr10I	RCCGGY	2 cut(s)	1076, 1147
Cfr13I	GGNCC	8 cut(s)	28, 82, 109, 510, 877, 957, 993, 1715
CpoI	CGGWCCG	1 cut(s)	82
CseI	GACGC	5 cut(s)	412, 567, 621, 753, 1607
Csp6I	GTAC	3 cut(s)	373, 433, 460
CspI	CGGWCCG	1 cut(s)	82
CviQI	GTAC	3 cut(s)	373, 433, 460
DdeI	CTNAG	2 cut(s)	484, 1856
DinI	GGCGCC	2 cut(s)	816, 1075
DraIII	CACNNNGTG	1 cut(s)	613
EaeI	YGGCCR	1 cut(s)	57
Eam1104I	CTCTTC	4 cut(s)	317, 452, 944, 1800
EarI	CTCTTC	4 cut(s)	317, 452, 944, 1800
EciI	GGCGGA	3 cut(s)	236, 999, 1062
Eco130I	CCWWGG	3 cut(s)	198, 1221, 1513
Eco24I	GRGCYC	4 cut(s)	392, 536, 824, 1929
Eco31I	GGTCTC	1 cut(s)	1533
Eco47I	GGWCC	6 cut(s)	28, 82, 109, 510, 877, 993
Eco72I	CACGTG	1 cut(s)	610
Eco88I	CYCGRG	4 cut(s)	293, 696, 901, 1922
EcoO109I	RGGNCCY	1 cut(s)	28
EcoRI	GAATTC	1 cut(s)	679
EcoRII	CCWGG	4 cut(s)	849, 959, 1291, 1523
EcoT14I	CCWWGG	3 cut(s)	198, 1221, 1513
EcoT22I	ATGCAT	1 cut(s)	1269
EcoT38I	GRGCYC	4 cut(s)	392, 536, 824, 1929
EgeI	GGCGCC	2 cut(s)	816, 1075
EheI	GGCGCC	2 cut(s)	816, 1075
ErhI	CCWWGG	3 cut(s)	198, 1221, 1513
Esp3I	CGTCTC	1 cut(s)	747
FaqI	GGGAC	1 cut(s)	1006
FauI	CCCGC	1 cut(s)	848
FauNDI	CATATG	1 cut(s)	1119
FbaI	TGATCA	3 cut(s)	3, 303, 1237
FblI	GTMKAC	1 cut(s)	1037
FokI	GGATG	5 cut(s)	313, 608, 1227, 1276, 1624
FriOI	GRGCYC	4 cut(s)	392, 536, 824, 1929
FspBI	CTAG	4 cut(s)	1041, 1449, 1628, 1930
GlaI	GCGC	2 cut(s)	816, 1075
GsaI	CCCAGC	1 cut(s)	1721
GsuI	CTGGAG	1 cut(s)	1489
HaeII	RGCGCY	2 cut(s)	818, 1077
HaeIII	GGCC	4 cut(s)	59, 71, 959, 1716
HgaI	GACGC	5 cut(s)	412, 567, 621, 753, 1607
HhaI	GCGC	2 cut(s)	817, 1076
Hin1I	GRCGYC	5 cut(s)	540, 632, 764, 815, 1074
Hin6I	GCGC	2 cut(s)	815, 1074
HinP1I	GCGC	2 cut(s)	815, 1074
HincII	GTYRAC	1 cut(s)	1180
HindII	GTYRAC	1 cut(s)	1180
HinfI	GANTC	8 cut(s)	224, 244, 352, 691, 1018, 1162, 1276, 1288
HpaI	GTTAAC	1 cut(s)	1180
HphI	GGTGA	4 cut(s)	94, 253, 628, 986
Hpy166II	GTNNAC	5 cut(s)	435, 871, 1038, 1180, 1843
Hpy188I	TCNGA	8 cut(s)	487, 538, 590, 1017, 1156, 1167, 1281, 1597
Hpy188III	TCNNGA	5 cut(s)	361, 478, 746, 1444, 1708
Hpy8I	GTNNAC	5 cut(s)	435, 871, 1038, 1180, 1843
Hpy99I	CGWCG	6 cut(s)	542, 545, 637, 640, 652, 889
HpyAV	CCTTC	4 cut(s)	283, 1054, 1227, 1328
HpyCH4III	ACNGT	2 cut(s)	997, 1370
HpyCH4IV	ACGT	3 cut(s)	54, 540, 609
HpyF10VI	GCNNNNNNNGC	8 cut(s)	129, 146, 812, 852, 861, 1426, 1688, 1727
HpyF3I	CTNAG	2 cut(s)	484, 1856
HpySE526I	ACGT	3 cut(s)	54, 540, 609
Hsp92I	GRCGYC	5 cut(s)	540, 632, 764, 815, 1074
HspAI	GCGC	2 cut(s)	815, 1074
KasI	GGCGCC	2 cut(s)	814, 1073
Ksp22I	TGATCA	3 cut(s)	3, 303, 1237
KspAI	GTTAAC	1 cut(s)	1180
LguI	GCTCTTC	3 cut(s)	317, 452, 1800
LmnI	GCTCC	7 cut(s)	122, 467, 539, 963, 1111, 1318, 1742
Lsp1109I	GCAGC	6 cut(s)	402, 1174, 1213, 1361, 1717, 1777
LweI	GCATC	4 cut(s)	132, 568, 1254, 1367
MaeI	CTAG	4 cut(s)	1041, 1449, 1628, 1930
MaeII	ACGT	3 cut(s)	54, 540, 609
MaeIII	GTNAC	2 cut(s)	603, 895
MbiI	CCGCTC	1 cut(s)	972
MfeI	CAATTG	1 cut(s)	1245
MflI	RGATCY	2 cut(s)	96, 1577
MhlI	GDGCHC	6 cut(s)	119, 392, 536, 824, 1845, 1929
MlsI	TGGCCA	1 cut(s)	59
MluCI	AATT	5 cut(s)	679, 913, 1245, 1271, 1566
MluNI	TGGCCA	1 cut(s)	59
Mly113I	GGCGCC	2 cut(s)	815, 1074
MlyI	GAGTC	3 cut(s)	218, 685, 1297
MmeI	TCCRAC	2 cut(s)	207, 561
Mox20I	TGGCCA	1 cut(s)	59
Mph1103I	ATGCAT	1 cut(s)	1269
MroXI	GAANNNNTTC	2 cut(s)	98, 1275
MscI	TGGCCA	1 cut(s)	59
MseI	TTAA	2 cut(s)	552, 1179
MslI	CAYNNNNRTG	4 cut(s)	440, 1466, 1543, 1556
Msp20I	TGGCCA	1 cut(s)	59
MspA1I	CMGCKG	1 cut(s)	9
MspR9I	CCNGG	8 cut(s)	107, 497, 508, 851, 961, 991, 1293, 1525
MunI	CAATTG	1 cut(s)	1245
Mva1269I	GAATGC	1 cut(s)	1884
MvaI	CCWGG	4 cut(s)	851, 961, 1293, 1525
MvnI	CGCG	2 cut(s)	218, 561
MwoI	GCNNNNNNNGC	8 cut(s)	129, 146, 812, 852, 861, 1426, 1688, 1727
NarI	GGCGCC	2 cut(s)	815, 1074
NciI	CCSGG	4 cut(s)	107, 497, 508, 991
NcoI	CCATGG	1 cut(s)	198
NdeI	CATATG	1 cut(s)	1119
NlaIV	GGNNCC	8 cut(s)	469, 511, 535, 816, 958, 994, 1075, 1579
NmeAIII	GCCGAG	2 cut(s)	607, 843
NmuCI	GTSAC	1 cut(s)	895
NsiI	ATGCAT	1 cut(s)	1269
NspI	RCATGY	3 cut(s)	1175, 1737, 1775
NspV	TTCGAA	2 cut(s)	704, 911
PaeI	GCATGC	1 cut(s)	1737
PaeR7I	CTCGAG	1 cut(s)	293
PagI	TCATGA	1 cut(s)	1443
PciSI	GCTCTTC	3 cut(s)	317, 452, 1800
PcsI	WCGNNNNNNNCGW	4 cut(s)	632, 644, 737, 881
PctI	GAATGC	1 cut(s)	1884
PdmI	GAANNNNTTC	2 cut(s)	98, 1275
PfeI	GAWTC	5 cut(s)	244, 352, 1018, 1162, 1276
PflFI	GACNNNGTC	1 cut(s)	997
PflMI	CCANNNNNTGG	1 cut(s)	1250
PfoI	TCCNGGA	1 cut(s)	495
PleI	GAGTC	3 cut(s)	218, 685, 1296
PluTI	GGCGCC	2 cut(s)	818, 1077
PmaCI	CACGTG	1 cut(s)	610
PmlI	CACGTG	1 cut(s)	610
PpsI	GAGTC	3 cut(s)	218, 685, 1296
Ppu21I	YACGTR	2 cut(s)	55, 610
PpuMI	RGGWCCY	1 cut(s)	28
PsiI	TTATAA	1 cut(s)	1571
Psp5II	RGGWCCY	1 cut(s)	28
Psp6I	CCWGG	4 cut(s)	849, 959, 1291, 1523
PspCI	CACGTG	1 cut(s)	610
PspFI	CCCAGC	1 cut(s)	1717
PspGI	CCWGG	4 cut(s)	849, 959, 1291, 1523
PspN4I	GGNNCC	8 cut(s)	469, 511, 535, 816, 958, 994, 1075, 1579
PspPI	GGNCC	8 cut(s)	28, 82, 109, 510, 877, 957, 993, 1715
PspPPI	RGGWCCY	1 cut(s)	28
PspXI	VCTCGAGB	1 cut(s)	293
PstI	CTGCAG	1 cut(s)	420
PstNI	CAGNNNCTG	1 cut(s)	1374
PsuI	RGATCY	2 cut(s)	96, 1577
PsyI	GACNNNGTC	1 cut(s)	997
RsaI	GTAC	3 cut(s)	374, 434, 461
RsaNI	GTAC	3 cut(s)	373, 433, 460
RseI	CAYNNNNRTG	4 cut(s)	440, 1466, 1543, 1556
Rsr2I	CGGWCCG	1 cut(s)	82
RsrII	CGGWCCG	1 cut(s)	82
SapI	GCTCTTC	3 cut(s)	317, 452, 1800
SaqAI	TTAA	2 cut(s)	552, 1179
Sau96I	GGNCC	8 cut(s)	28, 82, 109, 510, 877, 957, 993, 1715
SchI	GAGTC	3 cut(s)	218, 685, 1297
ScrFI	CCNGG	8 cut(s)	107, 497, 508, 851, 961, 991, 1293, 1525
SduI	GDGCHC	6 cut(s)	119, 392, 536, 824, 1845, 1929
SfaNI	GCATC	4 cut(s)	132, 568, 1254, 1367
SfcI	CTRYAG	1 cut(s)	416
SfoI	GGCGCC	2 cut(s)	816, 1075
Sfr274I	CTCGAG	1 cut(s)	293
SfuI	TTCGAA	2 cut(s)	704, 911
SgrAI	CRCCGGYG	1 cut(s)	1076
SinI	GGWCC	6 cut(s)	28, 82, 109, 510, 877, 993
SlaI	CTCGAG	1 cut(s)	293
SmiMI	CAYNNNNRTG	4 cut(s)	440, 1466, 1543, 1556
SmlI	CTYRAG	2 cut(s)	293, 1800
SmoI	CTYRAG	2 cut(s)	293, 1800
SpeI	ACTAGT	1 cut(s)	1040
SphI	GCATGC	1 cut(s)	1737
Sse9I	AATT	5 cut(s)	679, 913, 1245, 1271, 1566
SspDI	GGCGCC	2 cut(s)	814, 1073
SspI	AATATT	1 cut(s)	1590
SspMI	CTAG	4 cut(s)	1041, 1449, 1628, 1930
StyD4I	CCNGG	8 cut(s)	105, 495, 506, 849, 959, 989, 1291, 1523
StyI	CCWWGG	3 cut(s)	198, 1221, 1513
TaaI	ACNGT	2 cut(s)	997, 1370
TaiI	ACGT	3 cut(s)	57, 543, 612
TaqI	TCGA	8 cut(s)	294, 456, 526, 638, 704, 887, 911, 1136
TaqII	GACCGA	3 cut(s)	70, 590, 865
TasI	AATT	5 cut(s)	679, 913, 1245, 1271, 1566
TatI	WGTACW	1 cut(s)	459
TauI	GCSGC	5 cut(s)	49, 221, 632, 1071, 1074
TfiI	GAWTC	5 cut(s)	244, 352, 1018, 1162, 1276
Tru1I	TTAA	2 cut(s)	552, 1179
Tru9I	TTAA	2 cut(s)	552, 1179
TscAI	CASTG	3 cut(s)	119, 1104, 1867
TseFI	GTSAC	1 cut(s)	895
TseI	GCWGC	6 cut(s)	415, 1187, 1226, 1374, 1730, 1790
Tsp45I	GTSAC	1 cut(s)	895
TspDTI	ATGAA	8 cut(s)	153, 163, 202, 439, 947, 1106, 1284, 1627
TspGWI	ACGGA	2 cut(s)	570, 1416
TspRI	CASTG	3 cut(s)	119, 1104, 1867
Tth111I	GACNNNGTC	1 cut(s)	997
Van91I	CCANNNNNTGG	1 cut(s)	1250
VneI	GTGCAC	1 cut(s)	1841
VpaK11BI	GGWCC	6 cut(s)	28, 82, 109, 510, 877, 993
XapI	RAATTY	2 cut(s)	679, 913
XceI	RCATGY	3 cut(s)	1175, 1737, 1775
XcmI	CCANNNNNNNNNTGG	1 cut(s)	857
XhoI	CTCGAG	1 cut(s)	293
XmiI	GTMKAC	1 cut(s)	1037
XmnI	GAANNNNTTC	2 cut(s)	98, 1275
XspI	CTAG	4 cut(s)	1041, 1449, 1628, 1930
ZraI	GACGTC	1 cut(s)	541
Zsp2I	ATGCAT	1 cut(s)	1269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.