Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006848

Physical Location & Seq

Genomic Coordinates Forward (+)

26616630 .. 26620097

Gene Structure 3468 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1244500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1953 bp

ATGATTACTGGCAAGGACATTTACGATGTTCTTGCAGCCATAGTCCCCTTATACGTGGCTATGATCTTAGCCTACGGTTCCGTTAGGTGGTGGAAAATCTTCACGCCCGACCAATGCTCCGGCATAAACCGCTTCGTCGCTGTTTTCGCCGTCCCATTGCTCTCCTTCCACTTCGTATCCTCCAACGACCCTTACGCCATGAACTACCAATTCATCCTCGCCGACTCCCTCCAAAAAGTAGTCATCCTCGTTGCCCTCTTCCTCTGGCAAGCCTTCTCCAAGCGCGGCAACCTCGAGTGGATGATCACGCTCTTCTCCCTCTCCACCCTCCCCAACACCCTCGTCATGGGCATCCCTCTTTTACGCGCCATGTATGGCGACTTCTCCGGGACGCTGATGGTTCAGATTGTCGTTTTGCAGAGCGTGGTCTGGTACACTCTCATGCTCTTCATGTTCGAGTACCACGGCGCGAAGCTGCTCATCTCTGAGCAGTTCCCGGAGACTGCAGCTTCGATCACGTCGTTCAAAGTTGACTCTGACGTCGTTTCGCTCAACGGTCGAGAGCCGCTGCAAACCGACGCGGAAATCAGCGATGACGGGAAGCTTCACGTGGTGGTGAAAAGATCGAATGCGTCGTCTATGGTATCTTCTTTCAACAAGTCACACGGGTTGAACTCTTTGAATTCGATGACCCCACGGGCTTCGAATCTCACGGGAGTCGAGATTTACTCGGTGCAGTCCTCTCGCGAGCCCACCCCGCGGGCTTCCAGCTTCAACCAGACGGATTTTTACGCCATGTTTAACGCCAGCAAGGCCCCAAGCCCCAAACACGGATATACAAATAGCTTTCAAGGCGGATTCGGGGACGTTTATTCGGTGCAGTCATCCGAAGGGGTGACGCCGACAACTTCGAATTTCGATGAGGAGGCGATGAAGATGAGTAATATGAGTAATAAGAAGAGCGGGGCGAGGAGCATGAGTGGTGAGATCTTCATTGGTGGTCCGGCCTCTTCATACCTGCCTCCGAATCCCTTGTTTTCAGGGTCTACGAGTGGCGGGCTGAAGAAGAAAGATAGCGCTAGTGGTGGCGGTGGTGGTAGTGGTGGGGCATCGCCTAATAAGGAGCCTCATATGTTTGTTTGGAGTTCAAATGCATCGCCAGTTTCCGAAGGGAATCTTAGACATGCAATTAATCGAGCTGCTTCCACTGACTATGGCGCTACCGATCCTTCCAAGGTTGCAGGTCTTCGTAAACATGATAGTGCTGCTGCATCGAAAGGTATACACGAATTAATTGAGAATATGAGTCCTGGGAGTAAAATGAGAGGAGATAGCGAGCTTGAGATAGAAGAAGGATCGAATAAATTTCCACCGAGTGTGTCACCGTACAGTAGTAGTTGCCAAAAGAAGGTTGATATGGAAGGACGTGTAGCAAAGAAGCACCAGATGCCTCCAGCAAGTGTCATGACTAGGCTTATTCTCATCATGGTCTGGAGAAAGCTCATTAGAAACCCCAACACTTACTCCAGCCTTCTTGGCGTGGCATGGTCTCTCATATCATACAAGTGGAAAATCAAAATGCCAACAATTGTAAGTGGATCCATATCAATATTATCTGATGCTGGCTTAGGAATGGCTATGTTCAGTCTCGGATTATTCATGGCCTTACAACCAAAGATCATAGCTTGTGGAAAATCTGTTGCAACATTTGCAATGGCTGTTAGGTTCTTAACAGGCCCAGCAGTGATTGCTGCAACTTCTATTGCTGTCGGTCTTCGTGGAGTTCTTTTGCATGTTGCAATAGTTCAGGCTGCTCTTCCTCAAGGCATAGTTCCTTTTGTATTTGCCAAGGAATACAATGTCCACGCGGACATACTTAGCACTGCTGTTATTTTTGGAATGCTTGTTGCCTTGCCTATAACAATACTCTACTATATTCTTCTCGACCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001666 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005886 GO:0006810 GO:0006950 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009743 GO:0009746 GO:0009749 GO:0009914 GO:0009921 GO:0009925 GO:0009926 GO:0009958 GO:0009966 GO:0010033 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0015562 GO:0016020 GO:0016323 GO:0022857 GO:0023051 GO:0032991 GO:0034284 GO:0036293 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045178 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0065007 GO:0065008 GO:0070482 GO:0071944 GO:0080161 GO:0098590 GO:0098796 GO:0098797 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mem_trans

Protein Analysis

651

Amino Acids

70.22

Weight (kDa)

9.12

Isoelectric Point (pI)

46.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mem_trans	PF03547	10 - 645	1.7e-120	Membrane transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006848)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57090 AT5G57090
fragaria_vesca	FvH4_4g06870
malus_domestica	MD13G1239200.v1.1 MD16G1244500.v1.1
prunus_persica	Prupe.1G071700_v2.0.a1 Prupe.1G071800_v2.0.a1
pyrus_communis	pycom16g20590
rosa_chinensis	RchiOBHm_Chr4g0398751 RchiOBHm_Chr4g0398781
rosa_laevigata	RLG00000009323 RLG00000009324
rosa_multiflora	Rmu_sc0000458.1_g000007
rosa_roxburghii	Rroxscaffold_5G00343700 Rroxscaffold_5G00343710
rosa_rugosa	Rorug04G0011300 Rorug04G0011400
rosa_samantha	Rh4AG089200 Rh4AG089300 Rh4BG086600 Rh4CG097200 Rh4CG097400 Rh4DG080900 Rh4DG081100
rosa_wichuraiana	Rw4G007370 Rw4G007380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	539
AatII	GACGTC	1 cut(s)	543
Acc36I	ACCTGC	2 cut(s)	1026, 1232
AccBSI	CCGCTC	1 cut(s)	963
AccI	GTMKAC	2 cut(s)	1046, 1282
AccII	CGCG	7 cut(s)	285, 366, 470, 581, 747, 760, 1868
AclWI	GGATC	4 cut(s)	1220, 1363, 1593, 1606
AcsI	RAATTY	3 cut(s)	682, 913, 1364
AcuI	CTGAAG	1 cut(s)	1082
AcvI	CACGTG	1 cut(s)	610
AcyI	GRCGYC	2 cut(s)	540, 899
AdeI	CACNNNGTG	2 cut(s)	613, 1376
AfaI	GTAC	3 cut(s)	434, 461, 1388
AfeI	AGCGCT	1 cut(s)	1078
AfiI	CCNNNNNNNGG	5 cut(s)	87, 346, 759, 830, 1408
AflIII	ACRYGT	1 cut(s)	1426
AgsI	TTSAA	7 cut(s)	526, 655, 673, 682, 775, 851, 1149
AjiI	CACGTC	2 cut(s)	519, 1427
AjnI	CCWGG	1 cut(s)	1309
AluBI	AGCT	9 cut(s)	475, 509, 604, 771, 846, 1199, 1339, 1501, 1685
AluI	AGCT	9 cut(s)	475, 509, 604, 771, 846, 1199, 1339, 1501, 1685
Alw26I	GTCTC	3 cut(s)	494, 1554, 1652
AlwI	GGATC	4 cut(s)	1220, 1363, 1593, 1606
Ama87I	CYCGRG	1 cut(s)	293
Aor51HI	AGCGCT	1 cut(s)	1078
AoxI	GGCC	4 cut(s)	813, 1005, 1662, 1735
ApeKI	GCWGC	9 cut(s)	35, 475, 506, 568, 1199, 1265, 1268, 1751, 1811
ApoI	RAATTY	3 cut(s)	682, 913, 1364
ArsI	GACNNNNNNTTYG	4 cut(s)	493, 525, 620, 652
AseI	ATTAAT	2 cut(s)	1191, 1292
Asp700I	GAANNNNTTC	1 cut(s)	98
AspLEI	GCGC	5 cut(s)	285, 368, 470, 1079, 1220
AspS9I	GGNCC	3 cut(s)	814, 1001, 1736
AsuC2I	CCSGG	2 cut(s)	388, 497
AsuHPI	GGTGA	4 cut(s)	628, 907, 995, 1374
AsuII	TTCGAA	2 cut(s)	704, 911
AvaI	CYCGRG	1 cut(s)	293
AvaII	GGWCC	1 cut(s)	1001
BamHI	GGATCC	1 cut(s)	1598
BanII	GRGCYC	1 cut(s)	753
BarI	GAAGNNNNNNTAC	2 cut(s)	365, 397
BbrPI	CACGTG	1 cut(s)	610
BbsI	GAAGAC	2 cut(s)	1238, 1766
BbvI	GCAGC	9 cut(s)	47, 462, 518, 555, 1186, 1252, 1255, 1738, 1798
BccI	CCATC	1 cut(s)	391
BceAI	ACGGC	2 cut(s)	134, 481
BcgI	CGANNNNNNTGC	4 cut(s)	14, 48, 725, 759
BciT130I	CCWGG	1 cut(s)	1311
BciVI	GTATCC	1 cut(s)	187
BclI	TGATCA	1 cut(s)	303
BcnI	CCSGG	2 cut(s)	388, 497
BcoDI	GTCTC	3 cut(s)	494, 1554, 1652
BfaI	CTAG	3 cut(s)	1080, 1470, 1951
BfmI	CTRYAG	1 cut(s)	504
BfoI	RGCGCY	2 cut(s)	1080, 1221
BfuAI	ACCTGC	2 cut(s)	1026, 1232
BfuI	GTATCC	1 cut(s)	187
BglI	GCCNNNNNGGC	2 cut(s)	812, 1536
BglII	AGATCT	1 cut(s)	987
Bme1390I	CCNGG	3 cut(s)	388, 497, 1311
Bme18I	GGWCC	1 cut(s)	1001
BmeT110I	CYCGRG	1 cut(s)	293
BmgBI	CACGTC	2 cut(s)	519, 1427
BmgT120I	GGNCC	3 cut(s)	814, 1001, 1736
BmiI	GGNNCC	4 cut(s)	79, 816, 1125, 1600
BmrFI	CCNGG	3 cut(s)	388, 497, 1311
BmsI	GCATC	6 cut(s)	360, 1118, 1163, 1280, 1437, 1609
BpiI	GAAGAC	2 cut(s)	1238, 1766
BplI	GAGNNNNNCTC	2 cut(s)	713, 745
BpmI	CTGGAG	3 cut(s)	1437, 1510, 1513
Bpu10I	CCTNAGC	1 cut(s)	1627
Bpu14I	TTCGAA	2 cut(s)	704, 911
BpuEI	CTTGAG	2 cut(s)	1361, 1806
BpuMI	CCSGG	2 cut(s)	388, 497
BsaAI	YACGTR	2 cut(s)	55, 610
BsaBI	GATNNNNATC	1 cut(s)	1603
BsaHI	GRCGYC	2 cut(s)	540, 899
BsaI	GGTCTC	1 cut(s)	1554
BsaJI	CCNNGG	6 cut(s)	463, 695, 758, 1233, 1310, 1848
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 964, 994, 1508, 1538
Bsc4I	CCNNNNNNNGG	5 cut(s)	87, 346, 759, 830, 1408
Bse1I	ACTGG	2 cut(s)	13, 1160
Bse3DI	GCAATG	2 cut(s)	155, 1719
Bse8I	GATNNNNATC	1 cut(s)	1603
BseBI	CCWGG	1 cut(s)	1311
BseDI	CCNNGG	6 cut(s)	463, 695, 758, 1233, 1310, 1848
BseGI	GGATG	5 cut(s)	213, 243, 306, 351, 884
BseJI	GATNNNNATC	1 cut(s)	1603
BseLI	CCNNNNNNNGG	5 cut(s)	87, 346, 759, 830, 1408
BseMI	GCAATG	2 cut(s)	155, 1719
BseMII	CTCAG	1 cut(s)	477
BseNI	ACTGG	2 cut(s)	13, 1160
BseRI	GAGGAG	3 cut(s)	938, 985, 1341
BseXI	GCAGC	9 cut(s)	47, 462, 518, 555, 1186, 1252, 1255, 1738, 1798
BseYI	CCCAGC	1 cut(s)	1738
BsgI	GTGCAG	2 cut(s)	755, 899
Bsh1236I	CGCG	7 cut(s)	285, 366, 470, 581, 747, 760, 1868
Bsh1285I	CGRYCG	1 cut(s)	559
BshFI	GGCC	4 cut(s)	815, 1007, 1664, 1737
BsiEI	CGRYCG	1 cut(s)	559
BsiHKCI	CYCGRG	1 cut(s)	293
BsiSI	CCGG	4 cut(s)	120, 387, 497, 1004
BslFI	GGGAC	4 cut(s)	29, 137, 403, 878
BslI	CCNNNNNNNGG	5 cut(s)	87, 346, 759, 830, 1408
BsmAI	GTCTC	3 cut(s)	494, 1554, 1652
BsmFI	GGGAC	4 cut(s)	29, 137, 403, 878
BsmI	GAATGC	2 cut(s)	634, 1905
BsnI	GGCC	4 cut(s)	815, 1007, 1664, 1737
Bso31I	GGTCTC	1 cut(s)	1554
BsoBI	CYCGRG	1 cut(s)	293
Bsp119I	TTCGAA	2 cut(s)	704, 911
Bsp1286I	GDGCHC	1 cut(s)	753
Bsp143I	GATC	9 cut(s)	63, 303, 513, 623, 987, 1225, 1355, 1598, 1677
Bsp68I	TCGCGA	1 cut(s)	747
BspANI	GGCC	4 cut(s)	815, 1007, 1664, 1737
BspCNI	CTCAG	1 cut(s)	478
BspFNI	CGCG	7 cut(s)	285, 366, 470, 581, 747, 760, 1868
BspHI	TCATGA	1 cut(s)	1464
BspLI	GGNNCC	4 cut(s)	79, 816, 1125, 1600
BspMAI	CTGCAG	1 cut(s)	508
BspMI	ACCTGC	2 cut(s)	1026, 1232
BspPI	GGATC	4 cut(s)	1220, 1363, 1593, 1606
BspQI	GCTCTTC	4 cut(s)	317, 452, 953, 1821
BspT104I	TTCGAA	2 cut(s)	704, 911
BspTNI	GGTCTC	1 cut(s)	1554
BsrBI	CCGCTC	1 cut(s)	963
BsrDI	GCAATG	2 cut(s)	155, 1719
BsrI	ACTGG	2 cut(s)	13, 1160
BssECI	CCNNGG	6 cut(s)	463, 695, 758, 1233, 1310, 1848
BssMI	GATC	9 cut(s)	63, 303, 513, 623, 987, 1225, 1355, 1598, 1677
BssNAI	GTATAC	1 cut(s)	1283
BssNI	GRCGYC	2 cut(s)	540, 899
BssT1I	CCWWGG	2 cut(s)	1233, 1848
Bst1107I	GTATAC	1 cut(s)	1283
Bst2UI	CCWGG	1 cut(s)	1311
Bst4CI	ACNGT	4 cut(s)	77, 557, 1386, 1391
Bst6I	CTCTTC	6 cut(s)	263, 317, 452, 953, 1015, 1821
BstACI	GRCGYC	2 cut(s)	540, 899
BstAPI	GCANNNNNTGC	3 cut(s)	1447, 1709, 1748
BstBAI	YACGTR	2 cut(s)	55, 610
BstBI	TTCGAA	2 cut(s)	704, 911
BstC8I	GCNNGC	7 cut(s)	270, 749, 762, 808, 1058, 1337, 1624
BstDEI	CTNAG	5 cut(s)	67, 486, 1178, 1627, 1877
BstDSI	CCRYGG	3 cut(s)	463, 695, 758
BstF5I	GGATG	5 cut(s)	213, 243, 306, 351, 884
BstFNI	CGCG	7 cut(s)	285, 366, 470, 581, 747, 760, 1868
BstH2I	RGCGCY	2 cut(s)	1080, 1221
BstHHI	GCGC	5 cut(s)	285, 368, 470, 1079, 1220
BstKTI	GATC	9 cut(s)	66, 306, 516, 626, 990, 1228, 1358, 1601, 1680
BstMAI	GTCTC	3 cut(s)	494, 1554, 1652
BstMBI	GATC	9 cut(s)	63, 303, 513, 623, 987, 1225, 1355, 1598, 1677
BstMCI	CGRYCG	1 cut(s)	559
BstMWI	GCNNNNNNNGC	9 cut(s)	129, 146, 757, 812, 852, 1447, 1536, 1709, 1748
BstNI	CCWGG	1 cut(s)	1311
BstNSI	RCATGY	2 cut(s)	1187, 1796
BstSCI	CCNGG	3 cut(s)	386, 495, 1309
BstSFI	CTRYAG	1 cut(s)	504
BstUI	CGCG	7 cut(s)	285, 366, 470, 581, 747, 760, 1868
BstV1I	GCAGC	9 cut(s)	47, 462, 518, 555, 1186, 1252, 1255, 1738, 1798
BstV2I	GAAGAC	2 cut(s)	1238, 1766
BstX2I	RGATCY	2 cut(s)	987, 1598
BstYI	RGATCY	2 cut(s)	987, 1598
BstZ17I	GTATAC	1 cut(s)	1283
BsuI	GTATCC	1 cut(s)	187
BsuRI	GGCC	4 cut(s)	815, 1007, 1664, 1737
BtgI	CCRYGG	3 cut(s)	463, 695, 758
BtgZI	GCGATG	4 cut(s)	606, 944, 1095, 1140
BtrI	CACGTC	2 cut(s)	519, 1427
BtsCI	GGATG	5 cut(s)	213, 243, 306, 351, 884
BtsI	GCAGTG	2 cut(s)	1749, 1881
BtsIMutI	CAGTG	3 cut(s)	1206, 1749, 1881
BtuMI	TCGCGA	1 cut(s)	747
BveI	ACCTGC	2 cut(s)	1026, 1232
Cac8I	GCNNGC	7 cut(s)	270, 749, 762, 808, 1058, 1337, 1624
CciI	TCATGA	1 cut(s)	1464
CfoI	GCGC	5 cut(s)	285, 368, 470, 1079, 1220
Cfr13I	GGNCC	3 cut(s)	814, 1001, 1736
Cfr42I	CCGCGG	1 cut(s)	761
CseI	GACGC	4 cut(s)	400, 587, 621, 907
Csp6I	GTAC	3 cut(s)	433, 460, 1387
CviQI	GTAC	3 cut(s)	433, 460, 1387
DdeI	CTNAG	5 cut(s)	67, 486, 1178, 1627, 1877
DpnI	GATC	9 cut(s)	65, 305, 515, 625, 989, 1227, 1357, 1600, 1679
DpnII	GATC	9 cut(s)	63, 303, 513, 623, 987, 1225, 1355, 1598, 1677
DraIII	CACNNNGTG	2 cut(s)	613, 1376
DrdI	GACNNNNNNGTC	1 cut(s)	539
DseDI	GACNNNNNNGTC	1 cut(s)	539
Eam1104I	CTCTTC	6 cut(s)	263, 317, 452, 953, 1015, 1821
EarI	CTCTTC	6 cut(s)	263, 317, 452, 953, 1015, 1821
EciI	GGCGGA	1 cut(s)	870
Eco130I	CCWWGG	2 cut(s)	1233, 1848
Eco24I	GRGCYC	1 cut(s)	753
Eco31I	GGTCTC	1 cut(s)	1554
Eco47I	GGWCC	1 cut(s)	1001
Eco47III	AGCGCT	1 cut(s)	1078
Eco57I	CTGAAG	1 cut(s)	1082
Eco72I	CACGTG	1 cut(s)	610
Eco88I	CYCGRG	1 cut(s)	293
EcoO109I	RGGNCCY	1 cut(s)	814
EcoRI	GAATTC	1 cut(s)	682
EcoRII	CCWGG	1 cut(s)	1309
EcoT14I	CCWWGG	2 cut(s)	1233, 1848
EcoT22I	ATGCAT	1 cut(s)	1156
EcoT38I	GRGCYC	1 cut(s)	753
ErhI	CCWWGG	2 cut(s)	1233, 1848
FalI	AAGNNNNNCTT	2 cut(s)	1161, 1193
FaqI	GGGAC	4 cut(s)	29, 137, 403, 878
FauI	CCCGC	4 cut(s)	753, 765, 956, 1049
FauNDI	CATATG	1 cut(s)	1131
FbaI	TGATCA	1 cut(s)	303
FblI	GTMKAC	2 cut(s)	1046, 1282
FokI	GGATG	5 cut(s)	200, 230, 313, 338, 871
FriOI	GRGCYC	1 cut(s)	753
FspBI	CTAG	3 cut(s)	1080, 1470, 1951
GlaI	GCGC	5 cut(s)	284, 367, 469, 1078, 1219
GsaI	CCCAGC	1 cut(s)	1742
GsuI	CTGGAG	3 cut(s)	1437, 1510, 1513
HaeII	RGCGCY	2 cut(s)	1080, 1221
HaeIII	GGCC	4 cut(s)	815, 1007, 1664, 1737
HapII	CCGG	4 cut(s)	120, 387, 497, 1004
HgaI	GACGC	4 cut(s)	400, 587, 621, 907
HhaI	GCGC	5 cut(s)	285, 368, 470, 1079, 1220
Hin1I	GRCGYC	2 cut(s)	540, 899
Hin6I	GCGC	5 cut(s)	283, 366, 468, 1077, 1218
HinP1I	GCGC	5 cut(s)	283, 366, 468, 1077, 1218
HincII	GTYRAC	1 cut(s)	532
HindII	GTYRAC	1 cut(s)	532
HindIII	AAGCTT	1 cut(s)	602
HinfI	GANTC	8 cut(s)	224, 533, 706, 717, 858, 1027, 1174, 1306
HpaII	CCGG	4 cut(s)	120, 387, 497, 1004
HphI	GGTGA	4 cut(s)	628, 907, 995, 1374
Hpy166II	GTNNAC	6 cut(s)	435, 532, 1047, 1253, 1283, 1864
Hpy188I	TCNGA	8 cut(s)	405, 487, 538, 889, 1026, 1168, 1618, 1652
Hpy188III	TCNNGA	6 cut(s)	560, 721, 746, 1465, 1492, 1943
Hpy8I	GTNNAC	6 cut(s)	435, 532, 1047, 1253, 1283, 1864
Hpy99I	CGWCG	5 cut(s)	140, 523, 545, 581, 637
HpyAV	CCTTC	9 cut(s)	175, 283, 884, 1163, 1239, 1346, 1402, 1415, 1541
HpyCH4III	ACNGT	4 cut(s)	77, 557, 1386, 1391
HpyCH4IV	ACGT	6 cut(s)	54, 518, 540, 609, 867, 1426
HpyF10VI	GCNNNNNNNGC	9 cut(s)	129, 146, 757, 812, 852, 1447, 1536, 1709, 1748
HpyF3I	CTNAG	5 cut(s)	67, 486, 1178, 1627, 1877
HpySE526I	ACGT	6 cut(s)	54, 518, 540, 609, 867, 1426
Hsp92I	GRCGYC	2 cut(s)	540, 899
HspAI	GCGC	5 cut(s)	283, 366, 468, 1077, 1218
Ksp22I	TGATCA	1 cut(s)	303
KspI	CCGCGG	1 cut(s)	761
Kzo9I	GATC	9 cut(s)	63, 303, 513, 623, 987, 1225, 1355, 1598, 1677
LguI	GCTCTTC	4 cut(s)	317, 452, 953, 1821
LmnI	GCTCC	3 cut(s)	122, 972, 1123
Lsp1109I	GCAGC	9 cut(s)	47, 462, 518, 555, 1186, 1252, 1255, 1738, 1798
LweI	GCATC	6 cut(s)	360, 1118, 1163, 1280, 1437, 1609
MaeI	CTAG	3 cut(s)	1080, 1470, 1951
MaeII	ACGT	6 cut(s)	54, 518, 540, 609, 867, 1426
MaeIII	GTNAC	3 cut(s)	660, 895, 1380
MalI	GATC	9 cut(s)	65, 305, 515, 625, 989, 1227, 1357, 1600, 1679
MbiI	CCGCTC	1 cut(s)	963
MboI	GATC	9 cut(s)	63, 303, 513, 623, 987, 1225, 1355, 1598, 1677
MfeI	CAATTG	1 cut(s)	1587
MflI	RGATCY	2 cut(s)	987, 1598
MhlI	GDGCHC	1 cut(s)	753
MluCI	AATT	8 cut(s)	209, 682, 913, 1188, 1289, 1293, 1364, 1587
MlyI	GAGTC	4 cut(s)	218, 527, 726, 1315
MmeI	TCCRAC	1 cut(s)	207
Mph1103I	ATGCAT	1 cut(s)	1156
MroXI	GAANNNNTTC	1 cut(s)	98
MseI	TTAA	4 cut(s)	801, 1191, 1292, 1730
MslI	CAYNNNNRTG	3 cut(s)	440, 1260, 1564
MspA1I	CMGCKG	2 cut(s)	568, 760
MspI	CCGG	4 cut(s)	120, 387, 497, 1004
MspR9I	CCNGG	3 cut(s)	388, 497, 1311
MunI	CAATTG	1 cut(s)	1587
Mva1269I	GAATGC	2 cut(s)	634, 1905
MvaI	CCWGG	1 cut(s)	1311
MvnI	CGCG	7 cut(s)	285, 366, 470, 581, 747, 760, 1868
MwoI	GCNNNNNNNGC	9 cut(s)	129, 146, 757, 812, 852, 1447, 1536, 1709, 1748
NciI	CCSGG	2 cut(s)	388, 497
NdeI	CATATG	1 cut(s)	1131
NdeII	GATC	9 cut(s)	63, 303, 513, 623, 987, 1225, 1355, 1598, 1677
NlaIV	GGNNCC	4 cut(s)	79, 816, 1125, 1600
NmuCI	GTSAC	3 cut(s)	660, 895, 1380
NruI	TCGCGA	1 cut(s)	747
NsiI	ATGCAT	1 cut(s)	1156
NspI	RCATGY	2 cut(s)	1187, 1796
NspV	TTCGAA	2 cut(s)	704, 911
PaeR7I	CTCGAG	1 cut(s)	293
PagI	TCATGA	1 cut(s)	1464
PciSI	GCTCTTC	4 cut(s)	317, 452, 953, 1821
PcsI	WCGNNNNNNNCGW	2 cut(s)	518, 632
PctI	GAATGC	2 cut(s)	634, 1905
PdmI	GAANNNNTTC	1 cut(s)	98
PfeI	GAWTC	4 cut(s)	706, 858, 1027, 1174
PfoI	TCCNGGA	2 cut(s)	386, 495
PleI	GAGTC	4 cut(s)	218, 527, 725, 1314
PmaCI	CACGTG	1 cut(s)	610
PmlI	CACGTG	1 cut(s)	610
PpsI	GAGTC	4 cut(s)	218, 527, 725, 1314
Ppu21I	YACGTR	2 cut(s)	55, 610
PshBI	ATTAAT	2 cut(s)	1191, 1292
Psp6I	CCWGG	1 cut(s)	1309
PspCI	CACGTG	1 cut(s)	610
PspFI	CCCAGC	1 cut(s)	1738
PspGI	CCWGG	1 cut(s)	1309
PspN4I	GGNNCC	4 cut(s)	79, 816, 1125, 1600
PspPI	GGNCC	3 cut(s)	814, 1001, 1736
PspXI	VCTCGAGB	1 cut(s)	293
PstI	CTGCAG	1 cut(s)	508
PsuI	RGATCY	2 cut(s)	987, 1598
RruI	TCGCGA	1 cut(s)	747
RsaI	GTAC	3 cut(s)	434, 461, 1388
RsaNI	GTAC	3 cut(s)	433, 460, 1387
RseI	CAYNNNNRTG	3 cut(s)	440, 1260, 1564
SacII	CCGCGG	1 cut(s)	761
SapI	GCTCTTC	4 cut(s)	317, 452, 953, 1821
SaqAI	TTAA	4 cut(s)	801, 1191, 1292, 1730
Sau3AI	GATC	9 cut(s)	63, 303, 513, 623, 987, 1225, 1355, 1598, 1677
Sau96I	GGNCC	3 cut(s)	814, 1001, 1736
SchI	GAGTC	4 cut(s)	218, 527, 726, 1315
ScrFI	CCNGG	3 cut(s)	388, 497, 1311
SduI	GDGCHC	1 cut(s)	753
SfaNI	GCATC	6 cut(s)	360, 1118, 1163, 1280, 1437, 1609
SfcI	CTRYAG	1 cut(s)	504
Sfr274I	CTCGAG	1 cut(s)	293
Sfr303I	CCGCGG	1 cut(s)	761
SfuI	TTCGAA	2 cut(s)	704, 911
SgrBI	CCGCGG	1 cut(s)	761
SinI	GGWCC	1 cut(s)	1001
SlaI	CTCGAG	1 cut(s)	293
SmiMI	CAYNNNNRTG	3 cut(s)	440, 1260, 1564
SmlI	CTYRAG	3 cut(s)	293, 1340, 1821
SmoI	CTYRAG	3 cut(s)	293, 1340, 1821
Sse9I	AATT	8 cut(s)	209, 682, 913, 1188, 1289, 1293, 1364, 1587
SspI	AATATT	1 cut(s)	1611
SspMI	CTAG	3 cut(s)	1080, 1470, 1951
StyD4I	CCNGG	3 cut(s)	386, 495, 1309
StyI	CCWWGG	2 cut(s)	1233, 1848
TaaI	ACNGT	4 cut(s)	77, 557, 1386, 1391
TaiI	ACGT	6 cut(s)	57, 521, 543, 612, 870, 1429
TaqII	GACCGA	1 cut(s)	1760
TasI	AATT	8 cut(s)	209, 682, 913, 1188, 1289, 1293, 1364, 1587
TauI	GCSGC	2 cut(s)	288, 568
TfiI	GAWTC	4 cut(s)	706, 858, 1027, 1174
Tru1I	TTAA	4 cut(s)	801, 1191, 1292, 1730
Tru9I	TTAA	4 cut(s)	801, 1191, 1292, 1730
TscAI	CASTG	3 cut(s)	1213, 1749, 1888
TseFI	GTSAC	3 cut(s)	660, 895, 1380
TseI	GCWGC	9 cut(s)	35, 475, 506, 568, 1199, 1265, 1268, 1751, 1811
Tsp45I	GTSAC	3 cut(s)	660, 895, 1380
TspDTI	ATGAA	7 cut(s)	202, 215, 439, 947, 982, 1002, 1648
TspGWI	ACGGA	3 cut(s)	70, 797, 846
TspRI	CASTG	3 cut(s)	1213, 1749, 1888
VpaK11BI	GGWCC	1 cut(s)	1001
VspI	ATTAAT	2 cut(s)	1191, 1292
XapI	RAATTY	3 cut(s)	682, 913, 1364
XceI	RCATGY	2 cut(s)	1187, 1796
XhoI	CTCGAG	1 cut(s)	293
XmiI	GTMKAC	2 cut(s)	1046, 1282
XmnI	GAANNNNTTC	1 cut(s)	98
XspI	CTAG	3 cut(s)	1080, 1470, 1951
ZraI	GACGTC	1 cut(s)	541
Zsp2I	ATGCAT	1 cut(s)	1156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.