Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0006848

Physical Location & Seq

Genomic Coordinates Forward (+)

14889515 .. 14893938

Gene Structure 4424 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG086600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGATCAGCGGAAAGGACATTTACGAGGTCCTTGCAAGCATTGTGCCGCTTTACGTGGCCATGATCATGGCCTACGGCTCGGTCCGGTGGTGGAAGATCTTCACCCCGGACCAGTGCTCCGGCATCAACCGCTTCGTCGCGGTTTTTGCAGTTCCTCTGCTTTCATTTCACTTCATTTCTTCCAACGATCCCTACACCATGGACTACCAGTTCATCGCGGCGGACTCGTTGCAGAAAGTGGTGATTCTTGTGGCTCTATTCCTCTGGCAGACCTTCTCCAAGCGCGGCAACCTCGAGTGGATGATCACGCTCTTCTCCCTCTCCACTCTCCCCAACACTCTCGTCATGGGGATTCCTCTCTTGAAAGCCATGTACGGCGACTTTTCAGGCGCTCTCATGGTTCAAATCGTGGTACTGCAGAGCGTCATCTGGTACACTCTCATGCTCTTCATGTTCGAGTACAGAGGAGCCAAACTCTTGATCTCAGAGCAGTTCCCGGAAACCGCCGGGTCCATTACCTCGTTTCGAGTGGACTCCGACGTCGTTTCGCTTAACGGACGCGAGCCTTTGCAGACCGATGCCGAGATCGGAGAGGATGGAAAGTTACACGTGGTGGTGAGAAGATCGGCGGCGTCGTCGATGGTGTCGTCGTTCAAGTCGCACGGGTTGAACTCTTTGAATTCCGGCATGACTCCTCGGGCTTCGAACCTGACCGGCGTTGAGATTTACTCGGTTCAGTCCTCTCGTGAGCCGACACCGAGGGCGTCCAGCTTCAACCAGACTGACTTCTACGCCATGTTCAATGCCAGCAAGGCTCCGAGCCCTAAACACGGCTACACCAACAGCTTCCAGGGCGGGTTTGGTGATGTTTACTCAGTCCAGTCGTCGAAAGGCGTGACCCCGAGGACTTCGAATTTTGATGAGGAGATGATGAAGATAAGCAACAAAAAGAGAGGGGCCAGGAGCATGAGCGGTGAGCTGTTTAATGCCGGGACTGTGTCCTCGTATCCGCCTCCCAATCCCATGTTTTCGGGGTCTACTAGTGGCGGACCGAAAAAGAAGGAAAGCGGAGGCGCCGGTGCCGGTGGTGGTAGTGGTGGTGCAGTGCCTAATAAGGAGCTTCATATGTTTGTTTGGAGTTCGAGTGCTTCGCCGGTTTCCGAGGGGAATCTGAAACATGCAGTTAACCGAGCTGCCTCTACTGATTTTGGGGGCATTGATCCTTCCAAGGCTGCTCAACATGATCATCCAATTGCTGGTTCAAAAGGGATGCATGAATTGATTCAGAATATGAGTCCTGGTAGGAAAATGAGTGGAGATGGGGAGCTTGAAATAGAAGAAGGAACAAAGTTTCCGGCAAGTGCATCTCCATACAGCAGCTGCCAGAAGAAGATGGACATGGAAGACGGAGGTGTGGCAAAGAAGCAACAAATGCCTCCTGCAAGTGTCATGACTAGGCTTATACTCATCATGGTGTGGAGGAAGCTCATTAGAAATCCCAACACCTACTCCAGTCTCCTTGGTGTCGCATGGTCTCTCGTATCATACAAGTGGCACATCAAAATGCCCACAATTATAAGTGGATCCATATCAATATTATCTGACGCTGGCTTGGGGATGGCTATGTTCAGTCTAGGTTTATTCATGGCTTTACAACCAAAGATCATAGCTTGTGGAAAATCAGTAGCAACATTTGCAATGGCTGTTAGGTTCTTGACAGGCCCAGCAGTTATTGCTGCATGCTCCATAGCTGTTGGTCTTCGTGGAGTTCTTTTGCATGTTGCAATAGTTCAGGCTGCTCTTCCTCAAGGGATTGTTCCTTTTGTATTTGCCAAAGAATACAATGTGCACGCAGACATACTTAGCACTGCGGTCATTTTTGGAATGTTGATTGCCTTGCCTATAACAATACTCTACTATATTCTTCTCGGGCTCTATCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001666 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005886 GO:0006810 GO:0006950 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009743 GO:0009746 GO:0009749 GO:0009914 GO:0009921 GO:0009925 GO:0009926 GO:0009958 GO:0009966 GO:0010033 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0015562 GO:0016020 GO:0016323 GO:0022857 GO:0023051 GO:0032991 GO:0034284 GO:0036293 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045178 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0065007 GO:0065008 GO:0070482 GO:0071944 GO:0080161 GO:0098590 GO:0098796 GO:0098797 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mem_trans

Protein Analysis

647

Amino Acids

69.91

Weight (kDa)

9.16

Isoelectric Point (pI)

43.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mem_trans	PF03547	10 - 640	4.7e-121	Membrane transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006848)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57090 AT5G57090
fragaria_vesca	FvH4_4g06870
malus_domestica	MD13G1239200.v1.1 MD16G1244500.v1.1
prunus_persica	Prupe.1G071700_v2.0.a1 Prupe.1G071800_v2.0.a1
pyrus_communis	pycom16g20590
rosa_chinensis	RchiOBHm_Chr4g0398751 RchiOBHm_Chr4g0398781
rosa_laevigata	RLG00000009323 RLG00000009324
rosa_multiflora	Rmu_sc0000458.1_g000007
rosa_roxburghii	Rroxscaffold_5G00343700 Rroxscaffold_5G00343710
rosa_rugosa	Rorug04G0011300 Rorug04G0011400
rosa_samantha	Rh4AG089200 Rh4AG089300 Rh4BG086600 Rh4CG097200 Rh4CG097400 Rh4DG080900 Rh4DG081100
rosa_wichuraiana	Rw4G007370 Rw4G007380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1577
AasI	GACNNNNNNGTC	1 cut(s)	539
AatII	GACGTC	1 cut(s)	543
AccB1I	GGYRCC	2 cut(s)	1073, 1079
AccB7I	CCANNNNNTGG	1 cut(s)	1256
AccBSI	CCGCTC	1 cut(s)	972
AccI	GTMKAC	1 cut(s)	1037
AccII	CGCG	4 cut(s)	140, 218, 285, 561
AclWI	GGATC	4 cut(s)	182, 1214, 1578, 1591
AcoI	YGGCCR	1 cut(s)	57
AcsI	RAATTY	2 cut(s)	679, 913
AcvI	CACGTG	1 cut(s)	610
AcyI	GRCGYC	4 cut(s)	540, 632, 764, 1074
AdeI	CACNNNGTG	1 cut(s)	613
AfaI	GTAC	4 cut(s)	374, 414, 434, 461
AfiI	CCNNNNNNNGG	2 cut(s)	830, 1256
AflIII	ACRYGT	1 cut(s)	607
AgsI	TTSAA	9 cut(s)	364, 404, 655, 670, 679, 775, 802, 1263, 1331
AhlI	ACTAGT	1 cut(s)	1040
AjnI	CCWGG	3 cut(s)	849, 959, 1297
AjuI	GAANNNNNNNTTGG	2 cut(s)	1243, 1275
AloI	GAACNNNNNNTCC	4 cut(s)	194, 226, 1336, 1368
Alw21I	GWGCWC	2 cut(s)	119, 1851
Alw26I	GTCTC	2 cut(s)	1520, 1539
Alw44I	GTGCAC	1 cut(s)	1847
AlwI	GGATC	4 cut(s)	182, 1214, 1578, 1591
AlwNI	CAGNNNCTG	1 cut(s)	1380
Ama87I	CYCGRG	4 cut(s)	293, 696, 901, 1928
AoxI	GGCC	4 cut(s)	57, 69, 957, 1720
ApaLI	GTGCAC	1 cut(s)	1847
ApeKI	GCWGC	6 cut(s)	1193, 1232, 1377, 1380, 1736, 1796
ApoI	RAATTY	2 cut(s)	679, 913
Asp700I	GAANNNNTTC	2 cut(s)	98, 1281
AspLEI	GCGC	3 cut(s)	285, 392, 1076
AspS9I	GGNCC	7 cut(s)	28, 82, 109, 510, 957, 1049, 1721
AsuC2I	CCSGG	4 cut(s)	107, 497, 508, 991
AsuHPI	GGTGA	5 cut(s)	94, 253, 628, 875, 986
AsuII	TTCGAA	2 cut(s)	704, 911
AvaI	CYCGRG	4 cut(s)	293, 696, 901, 1928
AvaII	GGWCC	5 cut(s)	28, 82, 109, 510, 1049
BaeGI	GKGCMC	1 cut(s)	1851
BalI	TGGCCA	1 cut(s)	59
BamHI	GGATCC	1 cut(s)	1583
BanI	GGYRCC	2 cut(s)	1073, 1079
BanII	GRGCYC	2 cut(s)	824, 1935
BauI	CACGAG	1 cut(s)	744
BbrPI	CACGTG	1 cut(s)	610
BbsI	GAAGAC	2 cut(s)	1410, 1751
Bbv12I	GWGCWC	2 cut(s)	119, 1851
BbvI	GCAGC	6 cut(s)	1180, 1219, 1367, 1389, 1723, 1783
BccI	CCATC	5 cut(s)	590, 634, 1313, 1387, 1612
BceAI	ACGGC	3 cut(s)	91, 391, 847
BcgI	CGANNNNNNTGC	2 cut(s)	14, 48
BciT130I	CCWGG	3 cut(s)	851, 961, 1299
BciVI	GTATCC	1 cut(s)	1017
BclI	TGATCA	4 cut(s)	3, 63, 303, 1243
BcnI	CCSGG	4 cut(s)	107, 497, 508, 991
BcoDI	GTCTC	2 cut(s)	1520, 1539
BcuI	ACTAGT	1 cut(s)	1040
BfaI	CTAG	3 cut(s)	1041, 1455, 1634
BfmI	CTRYAG	1 cut(s)	416
BfoI	RGCGCY	2 cut(s)	393, 1077
BfuI	GTATCC	1 cut(s)	1017
BglI	GCCNNNNNGGC	1 cut(s)	812
BglII	AGATCT	1 cut(s)	96
Bme1390I	CCNGG	7 cut(s)	107, 497, 508, 851, 961, 991, 1299
Bme18I	GGWCC	5 cut(s)	28, 82, 109, 510, 1049
BmeT110I	CYCGRG	4 cut(s)	293, 696, 901, 1928
BmgT120I	GGNCC	7 cut(s)	28, 82, 109, 510, 957, 1049, 1721
BmiI	GGNNCC	7 cut(s)	469, 511, 816, 958, 1075, 1081, 1585
BmrFI	CCNGG	7 cut(s)	107, 497, 508, 851, 961, 991, 1299
BmsI	GCATC	4 cut(s)	132, 568, 1260, 1373
BpiI	GAAGAC	2 cut(s)	1410, 1751
BplI	GAGNNNNNCTC	4 cut(s)	459, 491, 713, 745
BpmI	CTGGAG	1 cut(s)	1495
Bpu14I	TTCGAA	2 cut(s)	704, 911
BpuEI	CTTGAG	1 cut(s)	1791
BpuMI	CCSGG	4 cut(s)	107, 497, 508, 991
BsaAI	YACGTR	2 cut(s)	55, 610
BsaBI	GATNNNNATC	1 cut(s)	1588
BsaHI	GRCGYC	4 cut(s)	540, 632, 764, 1074
BsaI	GGTCTC	1 cut(s)	1539
BsaJI	CCNNGG	9 cut(s)	105, 198, 695, 758, 850, 902, 1161, 1227, 1519
BsaWI	WCCGGW	1 cut(s)	84
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 1129, 1159, 1493, 1523
Bsc4I	CCNNNNNNNGG	2 cut(s)	830, 1256
Bse118I	RCCGGY	4 cut(s)	713, 1076, 1082, 1153
Bse1I	ACTGG	4 cut(s)	112, 208, 880, 1512
Bse3DI	GCAATG	1 cut(s)	1704
Bse8I	GATNNNNATC	1 cut(s)	1588
BseBI	CCWGG	3 cut(s)	851, 961, 1299
BseDI	CCNNGG	9 cut(s)	105, 198, 695, 758, 850, 902, 1161, 1227, 1519
BseGI	GGATG	5 cut(s)	306, 601, 1246, 1275, 1623
BseJI	GATNNNNATC	1 cut(s)	1588
BseLI	CCNNNNNNNGG	2 cut(s)	830, 1256
BseMI	GCAATG	1 cut(s)	1704
BseMII	CTCAG	2 cut(s)	498, 888
BseNI	ACTGG	4 cut(s)	112, 208, 880, 1512
BseRI	GAGGAG	3 cut(s)	480, 684, 938
BseSI	GKGCMC	1 cut(s)	1851
BseXI	GCAGC	6 cut(s)	1180, 1219, 1367, 1389, 1723, 1783
BseYI	CCCAGC	1 cut(s)	1723
BsgI	GTGCAG	1 cut(s)	1122
Bsh1236I	CGCG	4 cut(s)	140, 218, 285, 561
BshFI	GGCC	4 cut(s)	59, 71, 959, 1722
BshNI	GGYRCC	2 cut(s)	1073, 1079
BsiHKAI	GWGCWC	2 cut(s)	119, 1851
BsiHKCI	CYCGRG	4 cut(s)	293, 696, 901, 1928
BslFI	GGGAC	1 cut(s)	1006
BslI	CCNNNNNNNGG	2 cut(s)	830, 1256
BsmAI	GTCTC	2 cut(s)	1520, 1539
BsmFI	GGGAC	1 cut(s)	1006
BsnI	GGCC	4 cut(s)	59, 71, 959, 1722
Bso31I	GGTCTC	1 cut(s)	1539
BsoBI	CYCGRG	4 cut(s)	293, 696, 901, 1928
Bsp119I	TTCGAA	2 cut(s)	704, 911
Bsp1286I	GDGCHC	4 cut(s)	119, 824, 1851, 1935
Bsp19I	CCATGG	1 cut(s)	198
BspANI	GGCC	4 cut(s)	59, 71, 959, 1722
BspCNI	CTCAG	2 cut(s)	497, 887
BspFNI	CGCG	4 cut(s)	140, 218, 285, 561
BspHI	TCATGA	1 cut(s)	1449
BspLI	GGNNCC	7 cut(s)	469, 511, 816, 958, 1075, 1081, 1585
BspMAI	CTGCAG	1 cut(s)	420
BspPI	GGATC	4 cut(s)	182, 1214, 1578, 1591
BspQI	GCTCTTC	3 cut(s)	317, 452, 1806
BspT104I	TTCGAA	2 cut(s)	704, 911
BspT107I	GGYRCC	2 cut(s)	1073, 1079
BspTNI	GGTCTC	1 cut(s)	1539
BsrBI	CCGCTC	1 cut(s)	972
BsrDI	GCAATG	1 cut(s)	1704
BsrFI	RCCGGY	4 cut(s)	713, 1076, 1082, 1153
BsrI	ACTGG	4 cut(s)	112, 208, 880, 1512
BssAI	RCCGGY	4 cut(s)	713, 1076, 1082, 1153
BssECI	CCNNGG	9 cut(s)	105, 198, 695, 758, 850, 902, 1161, 1227, 1519
BssNI	GRCGYC	4 cut(s)	540, 632, 764, 1074
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	3 cut(s)	198, 1227, 1519
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	3 cut(s)	851, 961, 1299
Bst4CI	ACNGT	1 cut(s)	997
Bst6I	CTCTTC	3 cut(s)	317, 452, 1806
BstACI	GRCGYC	4 cut(s)	540, 632, 764, 1074
BstAPI	GCANNNNNTGC	3 cut(s)	1432, 1694, 1733
BstBAI	YACGTR	2 cut(s)	55, 610
BstBI	TTCGAA	2 cut(s)	704, 911
BstC8I	GCNNGC	6 cut(s)	37, 563, 808, 1609, 1741, 1851
BstDEI	CTNAG	3 cut(s)	484, 874, 1862
BstDSI	CCRYGG	1 cut(s)	198
BstF5I	GGATG	5 cut(s)	306, 601, 1246, 1275, 1623
BstFNI	CGCG	4 cut(s)	140, 218, 285, 561
BstH2I	RGCGCY	2 cut(s)	393, 1077
BstHHI	GCGC	3 cut(s)	285, 392, 1076
BstMAI	GTCTC	2 cut(s)	1520, 1539
BstMWI	GCNNNNNNNGC	7 cut(s)	129, 146, 812, 852, 1432, 1694, 1733
BstNI	CCWGG	3 cut(s)	851, 961, 1299
BstNSI	RCATGY	3 cut(s)	1181, 1743, 1781
BstSCI	CCNGG	7 cut(s)	105, 495, 506, 849, 959, 989, 1297
BstSFI	CTRYAG	1 cut(s)	416
BstSLI	GKGCMC	1 cut(s)	1851
BstUI	CGCG	4 cut(s)	140, 218, 285, 561
BstV1I	GCAGC	6 cut(s)	1180, 1219, 1367, 1389, 1723, 1783
BstV2I	GAAGAC	2 cut(s)	1410, 1751
BstX2I	RGATCY	2 cut(s)	96, 1583
BstXI	CCANNNNNNTGG	1 cut(s)	67
BstYI	RGATCY	2 cut(s)	96, 1583
BsuI	GTATCC	1 cut(s)	1017
BsuRI	GGCC	4 cut(s)	59, 71, 959, 1722
BtgI	CCRYGG	1 cut(s)	198
BtgZI	GCGATG	1 cut(s)	199
BtsCI	GGATG	5 cut(s)	306, 601, 1246, 1275, 1623
BtsI	GCAGTG	2 cut(s)	1110, 1866
BtsIMutI	CAGTG	3 cut(s)	119, 1110, 1866
Cac8I	GCNNGC	6 cut(s)	37, 563, 808, 1609, 1741, 1851
CaiI	CAGNNNCTG	1 cut(s)	1380
CciI	TCATGA	1 cut(s)	1449
CfoI	GCGC	3 cut(s)	285, 392, 1076
Cfr10I	RCCGGY	4 cut(s)	713, 1076, 1082, 1153
Cfr13I	GGNCC	7 cut(s)	28, 82, 109, 510, 957, 1049, 1721
CpoI	CGGWCCG	2 cut(s)	82, 1049
CseI	GACGC	5 cut(s)	412, 567, 621, 753, 1613
Csp6I	GTAC	4 cut(s)	373, 413, 433, 460
CspI	CGGWCCG	2 cut(s)	82, 1049
CviQI	GTAC	4 cut(s)	373, 413, 433, 460
DdeI	CTNAG	3 cut(s)	484, 874, 1862
DinI	GGCGCC	1 cut(s)	1075
DraIII	CACNNNGTG	1 cut(s)	613
DrdI	GACNNNNNNGTC	1 cut(s)	539
DseDI	GACNNNNNNGTC	1 cut(s)	539
EaeI	YGGCCR	1 cut(s)	57
Eam1104I	CTCTTC	3 cut(s)	317, 452, 1806
EarI	CTCTTC	3 cut(s)	317, 452, 1806
EciI	GGCGGA	3 cut(s)	236, 999, 1062
Eco130I	CCWWGG	3 cut(s)	198, 1227, 1519
Eco24I	GRGCYC	2 cut(s)	824, 1935
Eco31I	GGTCTC	1 cut(s)	1539
Eco47I	GGWCC	5 cut(s)	28, 82, 109, 510, 1049
Eco72I	CACGTG	1 cut(s)	610
Eco88I	CYCGRG	4 cut(s)	293, 696, 901, 1928
EcoO109I	RGGNCCY	1 cut(s)	28
EcoRI	GAATTC	1 cut(s)	679
EcoRII	CCWGG	3 cut(s)	849, 959, 1297
EcoT14I	CCWWGG	3 cut(s)	198, 1227, 1519
EcoT22I	ATGCAT	1 cut(s)	1275
EcoT38I	GRGCYC	2 cut(s)	824, 1935
EgeI	GGCGCC	1 cut(s)	1075
EheI	GGCGCC	1 cut(s)	1075
ErhI	CCWWGG	3 cut(s)	198, 1227, 1519
FaqI	GGGAC	1 cut(s)	1006
FauI	CCCGC	1 cut(s)	848
FauNDI	CATATG	1 cut(s)	1125
FbaI	TGATCA	4 cut(s)	3, 63, 303, 1243
FblI	GTMKAC	1 cut(s)	1037
FokI	GGATG	5 cut(s)	313, 608, 1233, 1282, 1630
FriOI	GRGCYC	2 cut(s)	824, 1935
FspBI	CTAG	3 cut(s)	1041, 1455, 1634
GlaI	GCGC	3 cut(s)	284, 391, 1075
GsaI	CCCAGC	1 cut(s)	1727
GsuI	CTGGAG	1 cut(s)	1495
HaeII	RGCGCY	2 cut(s)	393, 1077
HaeIII	GGCC	4 cut(s)	59, 71, 959, 1722
HgaI	GACGC	5 cut(s)	412, 567, 621, 753, 1613
HhaI	GCGC	3 cut(s)	285, 392, 1076
Hin1I	GRCGYC	4 cut(s)	540, 632, 764, 1074
Hin6I	GCGC	3 cut(s)	283, 390, 1074
HinP1I	GCGC	3 cut(s)	283, 390, 1074
HincII	GTYRAC	1 cut(s)	1186
HindII	GTYRAC	1 cut(s)	1186
HinfI	GANTC	8 cut(s)	224, 244, 352, 533, 691, 1168, 1282, 1294
HpaI	GTTAAC	1 cut(s)	1186
HphI	GGTGA	5 cut(s)	94, 253, 628, 875, 986
Hpy166II	GTNNAC	6 cut(s)	435, 532, 871, 1038, 1186, 1849
Hpy188I	TCNGA	8 cut(s)	487, 538, 590, 819, 1162, 1173, 1287, 1603
Hpy188III	TCNNGA	5 cut(s)	361, 478, 746, 1450, 1714
Hpy8I	GTNNAC	6 cut(s)	435, 532, 871, 1038, 1186, 1849
Hpy99I	CGWCG	7 cut(s)	140, 542, 545, 637, 640, 652, 889
HpyAV	CCTTC	4 cut(s)	283, 1054, 1233, 1334
HpyCH4III	ACNGT	1 cut(s)	997
HpyCH4IV	ACGT	3 cut(s)	54, 540, 609
HpyF10VI	GCNNNNNNNGC	7 cut(s)	129, 146, 812, 852, 1432, 1694, 1733
HpyF3I	CTNAG	3 cut(s)	484, 874, 1862
HpySE526I	ACGT	3 cut(s)	54, 540, 609
Hsp92I	GRCGYC	4 cut(s)	540, 632, 764, 1074
HspAI	GCGC	3 cut(s)	283, 390, 1074
KasI	GGCGCC	1 cut(s)	1073
Ksp22I	TGATCA	4 cut(s)	3, 63, 303, 1243
KspAI	GTTAAC	1 cut(s)	1186
LguI	GCTCTTC	3 cut(s)	317, 452, 1806
LmnI	GCTCC	7 cut(s)	122, 467, 820, 963, 1117, 1324, 1748
Lsp1109I	GCAGC	6 cut(s)	1180, 1219, 1367, 1389, 1723, 1783
LweI	GCATC	4 cut(s)	132, 568, 1260, 1373
MaeI	CTAG	3 cut(s)	1041, 1455, 1634
MaeII	ACGT	3 cut(s)	54, 540, 609
MaeIII	GTNAC	2 cut(s)	603, 895
MbiI	CCGCTC	1 cut(s)	972
MfeI	CAATTG	1 cut(s)	1251
MflI	RGATCY	2 cut(s)	96, 1583
MhlI	GDGCHC	4 cut(s)	119, 824, 1851, 1935
MlsI	TGGCCA	1 cut(s)	59
MluCI	AATT	5 cut(s)	679, 913, 1251, 1277, 1572
MluNI	TGGCCA	1 cut(s)	59
Mly113I	GGCGCC	1 cut(s)	1074
MlyI	GAGTC	4 cut(s)	218, 527, 685, 1303
MmeI	TCCRAC	2 cut(s)	207, 561
Mox20I	TGGCCA	1 cut(s)	59
Mph1103I	ATGCAT	1 cut(s)	1275
MroXI	GAANNNNTTC	2 cut(s)	98, 1281
MscI	TGGCCA	1 cut(s)	59
MseI	TTAA	3 cut(s)	552, 984, 1185
MslI	CAYNNNNRTG	5 cut(s)	65, 440, 1472, 1549, 1562
Msp20I	TGGCCA	1 cut(s)	59
MspA1I	CMGCKG	2 cut(s)	9, 1380
MspR9I	CCNGG	7 cut(s)	107, 497, 508, 851, 961, 991, 1299
MunI	CAATTG	1 cut(s)	1251
MvaI	CCWGG	3 cut(s)	851, 961, 1299
MvnI	CGCG	4 cut(s)	140, 218, 285, 561
MwoI	GCNNNNNNNGC	7 cut(s)	129, 146, 812, 852, 1432, 1694, 1733
NarI	GGCGCC	1 cut(s)	1074
NciI	CCSGG	4 cut(s)	107, 497, 508, 991
NcoI	CCATGG	1 cut(s)	198
NdeI	CATATG	1 cut(s)	1125
NlaIV	GGNNCC	7 cut(s)	469, 511, 816, 958, 1075, 1081, 1585
NmeAIII	GCCGAG	1 cut(s)	607
NmuCI	GTSAC	1 cut(s)	895
NsiI	ATGCAT	1 cut(s)	1275
NspI	RCATGY	3 cut(s)	1181, 1743, 1781
NspV	TTCGAA	2 cut(s)	704, 911
PaeI	GCATGC	1 cut(s)	1743
PaeR7I	CTCGAG	1 cut(s)	293
PagI	TCATGA	1 cut(s)	1449
PciSI	GCTCTTC	3 cut(s)	317, 452, 1806
PcsI	WCGNNNNNNNCGW	2 cut(s)	632, 644
PdmI	GAANNNNTTC	2 cut(s)	98, 1281
PfeI	GAWTC	4 cut(s)	244, 352, 1168, 1282
PflFI	GACNNNGTC	1 cut(s)	997
PflMI	CCANNNNNTGG	1 cut(s)	1256
PfoI	TCCNGGA	1 cut(s)	495
PleI	GAGTC	4 cut(s)	218, 527, 685, 1302
PluTI	GGCGCC	1 cut(s)	1077
PmaCI	CACGTG	1 cut(s)	610
PmlI	CACGTG	1 cut(s)	610
PpsI	GAGTC	4 cut(s)	218, 527, 685, 1302
Ppu21I	YACGTR	2 cut(s)	55, 610
PpuMI	RGGWCCY	1 cut(s)	28
PsiI	TTATAA	1 cut(s)	1577
Psp5II	RGGWCCY	1 cut(s)	28
Psp6I	CCWGG	3 cut(s)	849, 959, 1297
PspCI	CACGTG	1 cut(s)	610
PspFI	CCCAGC	1 cut(s)	1723
PspGI	CCWGG	3 cut(s)	849, 959, 1297
PspN4I	GGNNCC	7 cut(s)	469, 511, 816, 958, 1075, 1081, 1585
PspPI	GGNCC	7 cut(s)	28, 82, 109, 510, 957, 1049, 1721
PspPPI	RGGWCCY	1 cut(s)	28
PspXI	VCTCGAGB	1 cut(s)	293
PstI	CTGCAG	1 cut(s)	420
PstNI	CAGNNNCTG	1 cut(s)	1380
PsuI	RGATCY	2 cut(s)	96, 1583
PsyI	GACNNNGTC	1 cut(s)	997
PvuII	CAGCTG	1 cut(s)	1380
RsaI	GTAC	4 cut(s)	374, 414, 434, 461
RsaNI	GTAC	4 cut(s)	373, 413, 433, 460
RseI	CAYNNNNRTG	5 cut(s)	65, 440, 1472, 1549, 1562
Rsr2I	CGGWCCG	2 cut(s)	82, 1049
RsrII	CGGWCCG	2 cut(s)	82, 1049
SapI	GCTCTTC	3 cut(s)	317, 452, 1806
SaqAI	TTAA	3 cut(s)	552, 984, 1185
Sau96I	GGNCC	7 cut(s)	28, 82, 109, 510, 957, 1049, 1721
SchI	GAGTC	4 cut(s)	218, 527, 685, 1303
ScrFI	CCNGG	7 cut(s)	107, 497, 508, 851, 961, 991, 1299
SduI	GDGCHC	4 cut(s)	119, 824, 1851, 1935
SfaNI	GCATC	4 cut(s)	132, 568, 1260, 1373
SfcI	CTRYAG	1 cut(s)	416
SfoI	GGCGCC	1 cut(s)	1075
Sfr274I	CTCGAG	1 cut(s)	293
SfuI	TTCGAA	2 cut(s)	704, 911
SgrAI	CRCCGGYG	1 cut(s)	1076
SinI	GGWCC	5 cut(s)	28, 82, 109, 510, 1049
SlaI	CTCGAG	1 cut(s)	293
SmiMI	CAYNNNNRTG	5 cut(s)	65, 440, 1472, 1549, 1562
SmlI	CTYRAG	2 cut(s)	293, 1806
SmoI	CTYRAG	2 cut(s)	293, 1806
SpeI	ACTAGT	1 cut(s)	1040
SphI	GCATGC	1 cut(s)	1743
Sse9I	AATT	5 cut(s)	679, 913, 1251, 1277, 1572
SspDI	GGCGCC	1 cut(s)	1073
SspI	AATATT	1 cut(s)	1596
SspMI	CTAG	3 cut(s)	1041, 1455, 1634
StyD4I	CCNGG	7 cut(s)	105, 495, 506, 849, 959, 989, 1297
StyI	CCWWGG	3 cut(s)	198, 1227, 1519
TaaI	ACNGT	1 cut(s)	997
TaiI	ACGT	3 cut(s)	57, 543, 612
TaqI	TCGA	8 cut(s)	294, 456, 526, 638, 704, 887, 911, 1142
TaqII	GACCGA	3 cut(s)	70, 590, 1066
TasI	AATT	5 cut(s)	679, 913, 1251, 1277, 1572
TatI	WGTACW	1 cut(s)	459
TauI	GCSGC	4 cut(s)	49, 221, 288, 632
TfiI	GAWTC	4 cut(s)	244, 352, 1168, 1282
Tru1I	TTAA	3 cut(s)	552, 984, 1185
Tru9I	TTAA	3 cut(s)	552, 984, 1185
TscAI	CASTG	3 cut(s)	119, 1110, 1873
TseFI	GTSAC	1 cut(s)	895
TseI	GCWGC	6 cut(s)	1193, 1232, 1377, 1380, 1736, 1796
Tsp45I	GTSAC	1 cut(s)	895
TspDTI	ATGAA	8 cut(s)	153, 163, 202, 439, 947, 1112, 1290, 1633
TspGWI	ACGGA	2 cut(s)	570, 1422
TspRI	CASTG	3 cut(s)	119, 1110, 1873
Tth111I	GACNNNGTC	1 cut(s)	997
Van91I	CCANNNNNTGG	1 cut(s)	1256
VneI	GTGCAC	1 cut(s)	1847
VpaK11BI	GGWCC	5 cut(s)	28, 82, 109, 510, 1049
XapI	RAATTY	2 cut(s)	679, 913
XceI	RCATGY	3 cut(s)	1181, 1743, 1781
XcmI	CCANNNNNNNNNTGG	1 cut(s)	857
XhoI	CTCGAG	1 cut(s)	293
XmiI	GTMKAC	1 cut(s)	1037
XmnI	GAANNNNTTC	2 cut(s)	98, 1281
XspI	CTAG	3 cut(s)	1041, 1455, 1634
ZraI	GACGTC	1 cut(s)	541
Zsp2I	ATGCAT	1 cut(s)	1275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.