MD13G1241200.v1.1

Plant intracellular ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014334
Physical Location & Seq
Forward (+)
25052470 .. 25055454
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1241200.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGATGTACGAGCAGCAGCAACAGCAGCAGCAGCAAATTAGAATGGACACGAAGAAAAAAGCTGCTCATGAGAGGAAGAGATCGATAGAAGAAGAGAGGCTTGAAATTGTGGATTTGAGTGGGATGTCTCTTGATGCCCTTCCCAATCCTTCCCTTAATTTGGGGACTATTTGCAAGCTTGACCTTTCCAATAACAATCTTCAGAACATACCAGAATCCTTAACAGCAAGATTACTAAACGTGGTGGTGCTAGACGTGCACTCAAATCAGCTGAAATCTCTTCCAAACTCAATTGGTTGTTTGTCCAAGCTTAAGGTTCTCAATGTTGCCGGAAACCTTCTTTCATATCTCCCAAAAACTATTGAAAATTGCAGGTCAATGGAAGAGCTGAATGCCAACTTCAACAAGCTGAGCCACCTCCCAGACACCATTGGTTTTGAACTCAATAGCCTCAAGAAGCTATCAGTGAACTCCAACAAGCTTGTCTTTCTACCACGCTCCCTCACCCATCTTACGTCTCTGCACGTACTCGATGCTCGTCTCAACTGCCTCAGGTCCCTTCCTGAAGATCTCGAGAACCTTATCAACCTTGAAGTTCTCAATGTAAGCCAAAACTTCCAGTACCTCGAAACCCTACCGTACTCCATTGGCCTCCTTTTGTCTCTAGTCGAATTGGACGTCAGCTACAACAAGATCACAGCCCTACCAGACTCCATTGGATGCCTCAAGAAGCTTCAGAAGCTTATTGTGGAAGGAAACCCTATGGTTTCTCCTCCTCCTGAGGTGTTTGAGCAAGGGTTGCATTCAGTGAAGGAGTATCTTAGTGAGAAAATGAATGGTGGCCACAAGACTCCGCCAAAGAAGAAGTCTTGGATGGGCAAGTTGGTGAAGTATGGCACGTTCAATGGGGCCAACCGAAGCGGACCTCGAGAGGAGAGAGGAGGGTTCATCATGCCCGAGTACCGCTCAATTGACGGCCTTGCTTCACCGAGGTATATGGGGATGTTCTCACCCCGTCGCCTCTTCTCTCCCCGTACTTACTTCAGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8

Protein Analysis

351

Amino Acids

39.52

Weight (kDa)

9.13

Isoelectric Point (pI)

51.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 59 - 114 4.7e-08 Leucine rich repeat
LRR_14 PF23598 60 - 132 1.9e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014334)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19330 AT4G29880
fragaria_vesca FvH4_4g06910
malus_domestica MD13G1241200.v1.1 MD16G1245200.v1.1
prunus_persica Prupe.1G072100_v2.0.a1
pyrus_communis pycom13g21190
rosa_chinensis RchiOBHm_Chr4g0398811
rosa_laevigata RLG00000009317
rosa_multiflora Rmu_sc0004751.1_g000026
rosa_roxburghii Rroxscaffold_5G00343770
rosa_rugosa Rorug04G0012000
rosa_samantha Rh4AG089700 Rh4BG087100 Rh4CG097700 Rh4DG081400
rosa_wichuraiana Rw4G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 681
Acc36I ACCTGC 1 cut(s) 363
AccBSI CCGCTC 1 cut(s) 966
AciI CCGC 3 cut(s) 854, 921, 964
AcoI YGGCCR 1 cut(s) 841
AcuI CTGAAG 4 cut(s) 185, 585, 719, 1027
AcyI GRCGYC 1 cut(s) 678
AfaI GTAC 6 cut(s) 8, 528, 623, 641, 962, 1036
AfiI CCNNNNNNNGG 1 cut(s) 160
AflII CTTAAG 1 cut(s) 311
AgsI TTSAA 6 cut(s) 104, 365, 403, 440, 593, 904
AjiI CACGTC 1 cut(s) 256
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 4 cut(s) 132, 522, 545, 666
Alw44I GTGCAC 1 cut(s) 257
Ama87I CYCGRG 3 cut(s) 572, 927, 956
AoxI GGCC 4 cut(s) 649, 841, 909, 976
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 6 cut(s) 13, 16, 25, 28, 31, 62
ArsI GACNNNNNNTTYG 4 cut(s) 663, 695, 851, 883
AspS9I GGNCC 3 cut(s) 555, 909, 923
AsuHPI GGTGA 4 cut(s) 496, 898, 978, 1002
AvaI CYCGRG 3 cut(s) 572, 927, 956
AvaII GGWCC 2 cut(s) 555, 923
AxyI CCTNAGG 2 cut(s) 551, 780
BaeGI GKGCMC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 6 cut(s) 25, 28, 37, 40, 43, 49
BccI CCATC 2 cut(s) 516, 868
BceAI ACGGC 1 cut(s) 991
BcoDI GTCTC 4 cut(s) 132, 522, 545, 666
BfaI CTAG 2 cut(s) 251, 665
BfrI CTTAAG 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 363
BglII AGATCT 1 cut(s) 568
BisI GCNGC 6 cut(s) 14, 17, 26, 29, 32, 63
BlpI GCTNAGC 1 cut(s) 410
BlsI GCNGC 6 cut(s) 15, 18, 27, 30, 33, 64
Bme18I GGWCC 2 cut(s) 555, 923
BmeT110I CYCGRG 3 cut(s) 572, 927, 956
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 3 cut(s) 555, 909, 923
BmiI GGNNCC 2 cut(s) 557, 910
BmsI GCATC 3 cut(s) 124, 523, 710
BplI GAGNNNNNCTC 2 cut(s) 950, 982
Bpu1102I GCTNAGC 1 cut(s) 410
BpuEI CTTGAG 2 cut(s) 437, 710
Bsa29I ATCGAT 1 cut(s) 83
BsaAI YACGTR 1 cut(s) 526
BsaHI GRCGYC 1 cut(s) 678
BsaJI CCNNGG 1 cut(s) 989
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse1I ACTGG 1 cut(s) 619
Bse21I CCTNAGG 2 cut(s) 551, 780
BseCI ATCGAT 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 989
BseGI GGATG 4 cut(s) 129, 725, 879, 1008
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMII CTCAG 3 cut(s) 401, 565, 771
BseNI ACTGG 1 cut(s) 619
BseRI GAGGAG 4 cut(s) 762, 765, 947, 954
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 6 cut(s) 25, 28, 37, 40, 43, 49
BsgI GTGCAG 1 cut(s) 506
BshFI GGCC 4 cut(s) 651, 843, 911, 978
BshVI ATCGAT 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 3 cut(s) 572, 927, 956
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 2 cut(s) 178, 541
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 4 cut(s) 132, 522, 545, 666
BsmBI CGTCTC 2 cut(s) 522, 545
BsmFI GGGAC 2 cut(s) 178, 541
BsmI GAATGC 2 cut(s) 397, 802
BsnI GGCC 4 cut(s) 651, 843, 911, 978
BsoBI CYCGRG 3 cut(s) 572, 927, 956
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 3 cut(s) 80, 568, 693
Bsp1720I GCTNAGC 1 cut(s) 410
BspACI CCGC 3 cut(s) 854, 921, 964
BspANI GGCC 4 cut(s) 651, 843, 911, 978
BspCNI CTCAG 3 cut(s) 402, 564, 772
BspDI ATCGAT 1 cut(s) 83
BspHI TCATGA 1 cut(s) 67
BspLI GGNNCC 2 cut(s) 557, 910
BspMI ACCTGC 1 cut(s) 363
BspQI GCTCTTC 1 cut(s) 378
BspTI CTTAAG 1 cut(s) 311
BsrBI CCGCTC 1 cut(s) 966
BsrI ACTGG 1 cut(s) 619
BssECI CCNNGG 1 cut(s) 989
BssMI GATC 3 cut(s) 80, 568, 693
BssNI GRCGYC 1 cut(s) 678
Bst4CI ACNGT 1 cut(s) 639
Bst6I CTCTTC 5 cut(s) 71, 87, 285, 378, 1028
BstACI GRCGYC 1 cut(s) 678
BstAFI CTTAAG 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 799
BstBAI YACGTR 1 cut(s) 526
BstC8I GCNNGC 1 cut(s) 176
BstDEI CTNAG 4 cut(s) 410, 551, 780, 821
BstF5I GGATG 4 cut(s) 129, 725, 879, 1008
BstKTI GATC 3 cut(s) 83, 571, 696
BstMAI GTCTC 4 cut(s) 132, 522, 545, 666
BstMBI GATC 3 cut(s) 80, 568, 693
BstMWI GCNNNNNNNGC 6 cut(s) 22, 25, 31, 256, 739, 799
BstSLI GKGCMC 1 cut(s) 261
BstV1I GCAGC 6 cut(s) 25, 28, 37, 40, 43, 49
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
Bsu15I ATCGAT 1 cut(s) 83
Bsu36I CCTNAGG 2 cut(s) 551, 780
BsuRI GGCC 4 cut(s) 651, 843, 911, 978
BsuTUI ATCGAT 1 cut(s) 83
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 4 cut(s) 129, 725, 879, 1008
BtsIMutI CAGTG 2 cut(s) 471, 813
BveI ACCTGC 1 cut(s) 363
Cac8I GCNNGC 1 cut(s) 176
CciI TCATGA 1 cut(s) 67
Cfr13I GGNCC 3 cut(s) 555, 909, 923
ClaI ATCGAT 1 cut(s) 83
Csp6I GTAC 6 cut(s) 7, 527, 622, 640, 961, 1035
CviAII CATG 2 cut(s) 68, 952
CviQI GTAC 6 cut(s) 7, 527, 622, 640, 961, 1035
DdeI CTNAG 4 cut(s) 410, 551, 780, 821
DpnI GATC 3 cut(s) 82, 570, 695
DpnII GATC 3 cut(s) 80, 568, 693
EaeI YGGCCR 1 cut(s) 841
Eam1104I CTCTTC 5 cut(s) 71, 87, 285, 378, 1028
EarI CTCTTC 5 cut(s) 71, 87, 285, 378, 1028
EciI GGCGGA 1 cut(s) 843
Eco47I GGWCC 2 cut(s) 555, 923
Eco57I CTGAAG 4 cut(s) 185, 585, 719, 1027
Eco81I CCTNAGG 2 cut(s) 551, 780
Eco88I CYCGRG 3 cut(s) 572, 927, 956
EcoO109I RGGNCCY 1 cut(s) 555
Esp3I CGTCTC 2 cut(s) 522, 545
FaeI CATG 2 cut(s) 71, 955
FaiI YATR 8 cut(s) 69, 209, 346, 764, 894, 953, 996, 998
FalI AAGNNNNNCTT 4 cut(s) 84, 116, 470, 502
FaqI GGGAC 2 cut(s) 178, 541
FatI CATG 2 cut(s) 67, 951
Fnu4HI GCNGC 6 cut(s) 14, 17, 26, 29, 32, 63
FokI GGATG 4 cut(s) 136, 732, 886, 1015
Fsp4HI GCNGC 6 cut(s) 14, 17, 26, 29, 32, 63
FspBI CTAG 2 cut(s) 251, 665
GluI GCNGC 6 cut(s) 14, 17, 26, 29, 32, 63
HaeIII GGCC 4 cut(s) 651, 843, 911, 978
HapII CCGG 1 cut(s) 330
Hin1I GRCGYC 1 cut(s) 678
Hin1II CATG 2 cut(s) 71, 955
HindIII AAGCTT 5 cut(s) 176, 308, 479, 731, 740
HinfI GANTC 3 cut(s) 215, 710, 850
HpaII CCGG 1 cut(s) 330
HphI GGTGA 4 cut(s) 496, 898, 978, 1002
Hpy166II GTNNAC 2 cut(s) 259, 469
Hpy188I TCNGA 2 cut(s) 204, 738
Hpy188III TCNNGA 9 cut(s) 68, 131, 454, 563, 572, 574, 727, 779, 929
Hpy8I GTNNAC 2 cut(s) 259, 469
Hpy99I CGWCG 1 cut(s) 1020
HpyAV CCTTC 6 cut(s) 149, 159, 347, 569, 746, 805
HpyCH4III ACNGT 1 cut(s) 639
HpyCH4IV ACGT 6 cut(s) 240, 255, 515, 525, 678, 899
HpyCH4V TGCA 5 cut(s) 174, 259, 372, 523, 802
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 25, 31, 256, 739, 799
HpyF3I CTNAG 4 cut(s) 410, 551, 780, 821
HpySE526I ACGT 6 cut(s) 240, 255, 515, 525, 678, 899
Hsp92I GRCGYC 1 cut(s) 678
Hsp92II CATG 2 cut(s) 71, 955
Kzo9I GATC 3 cut(s) 80, 568, 693
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 1 cut(s) 503
LpnPI CCDG 9 cut(s) 225, 343, 358, 435, 538, 576, 632, 720, 792
Lsp1109I GCAGC 6 cut(s) 25, 28, 37, 40, 43, 49
LweI GCATC 3 cut(s) 124, 523, 710
MaeI CTAG 2 cut(s) 251, 665
MaeII ACGT 6 cut(s) 240, 255, 515, 525, 678, 899
MalI GATC 3 cut(s) 82, 570, 695
MbiI CCGCTC 1 cut(s) 966
MboI GATC 3 cut(s) 80, 568, 693
MfeI CAATTG 2 cut(s) 291, 969
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 843
MluCI AATT 7 cut(s) 36, 105, 157, 291, 367, 671, 969
MluNI TGGCCA 1 cut(s) 843
MlyI GAGTC 2 cut(s) 704, 844
MmeI TCCRAC 1 cut(s) 498
Mox20I TGGCCA 1 cut(s) 843
MscI TGGCCA 1 cut(s) 843
MseI TTAA 3 cut(s) 156, 221, 312
Msp20I TGGCCA 1 cut(s) 843
MspA1I CMGCKG 1 cut(s) 271
MspCI CTTAAG 1 cut(s) 311
MspI CCGG 1 cut(s) 330
MunI CAATTG 2 cut(s) 291, 969
Mva1269I GAATGC 2 cut(s) 397, 802
MwoI GCNNNNNNNGC 6 cut(s) 22, 25, 31, 256, 739, 799
NdeII GATC 3 cut(s) 80, 568, 693
NlaIII CATG 2 cut(s) 71, 955
NlaIV GGNNCC 2 cut(s) 557, 910
PaeR7I CTCGAG 2 cut(s) 572, 927
PagI TCATGA 1 cut(s) 67
PciSI GCTCTTC 1 cut(s) 378
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PctI GAATGC 2 cut(s) 397, 802
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 6 cut(s) 15, 18, 27, 30, 33, 64
PleI GAGTC 2 cut(s) 704, 844
PpsI GAGTC 2 cut(s) 704, 844
Ppu21I YACGTR 1 cut(s) 526
PpuMI RGGWCCY 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 557, 910
PspPI GGNCC 3 cut(s) 555, 909, 923
PspPPI RGGWCCY 1 cut(s) 555
PsrI GAACNNNNNNTAC 2 cut(s) 884, 916
PsuI RGATCY 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 271
RsaI GTAC 6 cut(s) 8, 528, 623, 641, 962, 1036
RsaNI GTAC 6 cut(s) 7, 527, 622, 640, 961, 1035
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 3 cut(s) 156, 221, 312
SatI GCNGC 6 cut(s) 14, 17, 26, 29, 32, 63
Sau3AI GATC 3 cut(s) 80, 568, 693
Sau96I GGNCC 3 cut(s) 555, 909, 923
SchI GAGTC 2 cut(s) 704, 844
SduI GDGCHC 1 cut(s) 261
SfaNI GCATC 3 cut(s) 124, 523, 710
Sfr274I CTCGAG 2 cut(s) 572, 927
SinI GGWCC 2 cut(s) 555, 923
SlaI CTCGAG 2 cut(s) 572, 927
SmlI CTYRAG 5 cut(s) 311, 452, 572, 725, 927
SmoI CTYRAG 5 cut(s) 311, 452, 572, 725, 927
Sse9I AATT 7 cut(s) 36, 105, 157, 291, 367, 671, 969
SsiI CCGC 3 cut(s) 854, 921, 964
SspMI CTAG 2 cut(s) 251, 665
TaaI ACNGT 1 cut(s) 639
TaiI ACGT 6 cut(s) 243, 258, 518, 528, 681, 902
TaqI TCGA 6 cut(s) 83, 531, 573, 627, 669, 928
TasI AATT 7 cut(s) 36, 105, 157, 291, 367, 671, 969
TfiI GAWTC 1 cut(s) 215
Tru1I TTAA 3 cut(s) 156, 221, 312
Tru9I TTAA 3 cut(s) 156, 221, 312
TscAI CASTG 2 cut(s) 471, 813
TseI GCWGC 6 cut(s) 13, 16, 25, 28, 31, 62
TspDTI ATGAA 3 cut(s) 333, 848, 937
TspRI CASTG 2 cut(s) 471, 813
Vha464I CTTAAG 1 cut(s) 311
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 555, 923
XhoI CTCGAG 2 cut(s) 572, 927
XspI CTAG 2 cut(s) 251, 665
ZraI GACGTC 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.