Rw4G007410

Plant intracellular ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014334
Physical Location & Seq
Reverse (-)
15650936 .. 15653948
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007410.1

Sequence Viewer

Length: 1062 bp
ATGATGTACGAGCAACAGCAGCAACAACAGCAGCCGCAGCAAGTGAGGATGGATATCAGGAAGAAGGCTGGTCATGAGAGGAGGAAGTCGATGACCGAAGAAGAGAGGCTTGAGATTGTGGATTTGAGTGGGATGTCTCTTGATGCCTTGCCTAATCCCTCTCTCAATTTGGGGAGTATTTGCAAGCTTGATCTTTCCAATAACAATCTCCAGAACATACCAGAATCCTTGACAGCAAGGTTACTGAACGTGGTGGTGTTCGACGTGCACTCAAATCAGCTGAAGTCCCTTCCAAACTCAATTGGGTGTTTGTCAAAGCTCAAGATTCTCAATGTTTCTGGAAACCTTCTTGAATCTCTCCCCAAACCTATTGAAAATTGCAGGTCACTGGAAGAGTTGAATGCCAACTTCAACAAGATCAGCGTGCTCCCAGACACCATTGGATTCGAAGTTATCAACCTCAAGAAGCTATCCGTGAATTCCAACAAGCTTGTCTTCTTGCCACGCTCTCTCACCCACCTGACCAATCTGCGGGTACTAGATGCTCGCCTCAACTGCCTCAGGTCTCTTCCTGAAGACCTTGAGAACCTCATCAACCTCGAAGTTCTCAATGTCAGCCAAAACTTCCAGTACCTGGAAACCCTACCCTACTCCATTGGCCTCCTCATGTCCCTTGTCGAACTGGACGTCAGCTACAACAAGATCACCGCCCTCCCGGACTCCATCGGATGTCTCAAGAAGCTCCAGAAGCTCATTGTCGAAGGGAACCCTATGGTCTCGCCTCCAGTCGAGGTTTTCGAGCAGGGTTTGCATGCGGTGAGGGAGTACCTAAGTGAGAAAATGAATGGTGGACACAAGAGTCCAGCCAAGAAGAAATCTTGGGTGGGGAAGTTGGTGAAGTATGGCACCTTCAACGGGGCCAACAGAAGCGTCTCTCGAGAGGAGAGAGAAGGGTACATCATGCCTGAGTACCGCTCCATCGATGGCCTTGCTTCCCCGAGGTATATGGGAATGTTCTCTCCCCGTCGTCTATTCTCGCCTCGTCATTACTTCAGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8

Protein Analysis

353

Amino Acids

40.01

Weight (kDa)

9.05

Isoelectric Point (pI)

55.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 62 - 117 9.6e-08 Leucine rich repeat
LRR_14 PF23598 63 - 135 1.4e-06 Leucine-rich repeat region
LRR_8 PF13855 128 - 183 8e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014334)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19330 AT4G29880
fragaria_vesca FvH4_4g06910
malus_domestica MD13G1241200.v1.1 MD16G1245200.v1.1
prunus_persica Prupe.1G072100_v2.0.a1
pyrus_communis pycom13g21190
rosa_chinensis RchiOBHm_Chr4g0398811
rosa_laevigata RLG00000009317
rosa_multiflora Rmu_sc0004751.1_g000026
rosa_roxburghii Rroxscaffold_5G00343770
rosa_rugosa Rorug04G0012000
rosa_samantha Rh4AG089700 Rh4BG087100 Rh4CG097700 Rh4DG081400
rosa_wichuraiana Rw4G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 858
AatII GACGTC 1 cut(s) 690
Acc36I ACCTGC 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 905
AccB7I CCANNNNNTGG 1 cut(s) 634
AccBSI CCGCTC 1 cut(s) 975
AciI CCGC 5 cut(s) 35, 532, 708, 815, 973
AcsI RAATTY 1 cut(s) 478
AcuI CTGAAG 3 cut(s) 302, 594, 1036
AcyI GRCGYC 1 cut(s) 687
AfaI GTAC 6 cut(s) 8, 537, 632, 827, 956, 971
AfiI CCNNNNNNNGG 3 cut(s) 531, 634, 915
AgsI TTSAA 5 cut(s) 353, 374, 400, 412, 913
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 1 cut(s) 633
AluBI AGCT 8 cut(s) 187, 280, 319, 469, 490, 693, 742, 751
AluI AGCT 8 cut(s) 187, 280, 319, 469, 490, 693, 742, 751
Alw21I GWGCWC 2 cut(s) 270, 429
Alw26I GTCTC 5 cut(s) 141, 570, 737, 781, 937
Alw44I GTGCAC 1 cut(s) 266
AlwNI CAGNNNCTG 1 cut(s) 634
Ama87I CYCGRG 2 cut(s) 936, 997
AoxI GGCC 3 cut(s) 658, 918, 985
ApaLI GTGCAC 1 cut(s) 266
ApeKI GCWGC 3 cut(s) 19, 31, 37
ApoI RAATTY 1 cut(s) 478
ArsI GACNNNNNNTTYG 2 cut(s) 672, 704
AspS9I GGNCC 1 cut(s) 918
AsuC2I CCSGG 1 cut(s) 716
AsuHPI GGTGA 4 cut(s) 505, 697, 829, 907
AsuII TTCGAA 1 cut(s) 447
AvaI CYCGRG 2 cut(s) 936, 997
AxyI CCTNAGG 1 cut(s) 560
BaeGI GKGCMC 1 cut(s) 270
BanI GGYRCC 1 cut(s) 905
BarI GAAGNNNNNNTAC 2 cut(s) 893, 925
BbsI GAAGAC 2 cut(s) 487, 582
Bbv12I GWGCWC 2 cut(s) 270, 429
BbvI GCAGC 3 cut(s) 31, 43, 49
BccI CCATC 4 cut(s) 43, 731, 977, 986
BcgI CGANNNNNNTGC 2 cut(s) 971, 1005
BciT130I CCWGG 1 cut(s) 635
BcnI CCSGG 1 cut(s) 716
BcoDI GTCTC 5 cut(s) 141, 570, 737, 781, 937
BfaI CTAG 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 372
BisI GCNGC 4 cut(s) 20, 32, 35, 38
BlsI GCNGC 4 cut(s) 21, 33, 36, 39
Bme1390I CCNGG 2 cut(s) 635, 716
BmeT110I CYCGRG 2 cut(s) 936, 997
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 918
BmiI GGNNCC 3 cut(s) 767, 907, 919
BmrFI CCNGG 2 cut(s) 635, 716
BmsI GCATC 2 cut(s) 133, 532
BpiI GAAGAC 2 cut(s) 487, 582
BplI GAGNNNNNCTC 2 cut(s) 959, 991
BpmI CTGGAG 3 cut(s) 194, 728, 768
Bpu14I TTCGAA 1 cut(s) 447
BpuEI CTTGAG 5 cut(s) 131, 305, 446, 602, 719
BpuMI CCSGG 1 cut(s) 716
Bsa29I ATCGAT 1 cut(s) 981
BsaBI GATNNNNATC 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 687
BsaI GGTCTC 2 cut(s) 570, 781
BsaJI CCNNGG 1 cut(s) 998
Bsc4I CCNNNNNNNGG 3 cut(s) 531, 634, 915
Bse1I ACTGG 4 cut(s) 393, 628, 687, 785
Bse21I CCTNAGG 1 cut(s) 560
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 1 cut(s) 635
BseCI ATCGAT 1 cut(s) 981
BseDI CCNNGG 1 cut(s) 998
BseGI GGATG 3 cut(s) 54, 138, 734
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 3 cut(s) 531, 634, 915
BseMII CTCAG 2 cut(s) 574, 957
BseNI ACTGG 4 cut(s) 393, 628, 687, 785
BseRI GAGGAG 3 cut(s) 94, 653, 956
BseSI GKGCMC 1 cut(s) 270
BseXI GCAGC 3 cut(s) 31, 43, 49
BshFI GGCC 3 cut(s) 660, 920, 987
BshNI GGYRCC 1 cut(s) 905
BshVI ATCGAT 1 cut(s) 981
BsiHKAI GWGCWC 2 cut(s) 270, 429
BsiHKCI CYCGRG 2 cut(s) 936, 997
BsiSI CCGG 1 cut(s) 716
BslFI GGGAC 2 cut(s) 271, 655
BslI CCNNNNNNNGG 3 cut(s) 531, 634, 915
BsmAI GTCTC 5 cut(s) 141, 570, 737, 781, 937
BsmBI CGTCTC 1 cut(s) 937
BsmFI GGGAC 2 cut(s) 271, 655
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 3 cut(s) 660, 920, 987
Bso31I GGTCTC 2 cut(s) 570, 781
BsoBI CYCGRG 2 cut(s) 936, 997
Bsp119I TTCGAA 1 cut(s) 447
Bsp1286I GDGCHC 2 cut(s) 270, 429
Bsp143I GATC 3 cut(s) 190, 417, 702
BspACI CCGC 5 cut(s) 35, 532, 708, 815, 973
BspANI GGCC 3 cut(s) 660, 920, 987
BspCNI CTCAG 2 cut(s) 573, 958
BspDI ATCGAT 1 cut(s) 981
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 3 cut(s) 767, 907, 919
BspMI ACCTGC 1 cut(s) 372
BspT104I TTCGAA 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 905
BspTNI GGTCTC 2 cut(s) 570, 781
BsrBI CCGCTC 1 cut(s) 975
BsrI ACTGG 4 cut(s) 393, 628, 687, 785
BssECI CCNNGG 1 cut(s) 998
BssMI GATC 3 cut(s) 190, 417, 702
BssNI GRCGYC 1 cut(s) 687
Bst2UI CCWGG 1 cut(s) 635
Bst6I CTCTTC 3 cut(s) 96, 387, 573
BstACI GRCGYC 1 cut(s) 687
BstAPI GCANNNNNTGC 1 cut(s) 808
BstBI TTCGAA 1 cut(s) 447
BstC8I GCNNGC 4 cut(s) 185, 425, 547, 813
BstDEI CTNAG 3 cut(s) 560, 830, 966
BstF5I GGATG 3 cut(s) 54, 138, 734
BstKTI GATC 3 cut(s) 193, 420, 705
BstMAI GTCTC 5 cut(s) 141, 570, 737, 781, 937
BstMBI GATC 3 cut(s) 190, 417, 702
BstMWI GCNNNNNNNGC 6 cut(s) 19, 28, 37, 555, 748, 808
BstNI CCWGG 1 cut(s) 635
BstNSI RCATGY 1 cut(s) 815
BstSCI CCNGG 2 cut(s) 633, 714
BstSLI GKGCMC 1 cut(s) 270
BstV1I GCAGC 3 cut(s) 31, 43, 49
BstV2I GAAGAC 2 cut(s) 487, 582
Bsu15I ATCGAT 1 cut(s) 981
Bsu36I CCTNAGG 1 cut(s) 560
BsuRI GGCC 3 cut(s) 660, 920, 987
BsuTUI ATCGAT 1 cut(s) 981
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 3 cut(s) 54, 138, 734
BtsIMutI CAGTG 1 cut(s) 386
BveI ACCTGC 1 cut(s) 372
Cac8I GCNNGC 4 cut(s) 185, 425, 547, 813
CaiI CAGNNNCTG 1 cut(s) 634
CciI TCATGA 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 918
ClaI ATCGAT 1 cut(s) 981
CseI GACGC 1 cut(s) 919
Csp6I GTAC 6 cut(s) 7, 536, 631, 826, 955, 970
CviAII CATG 4 cut(s) 74, 667, 812, 961
CviQI GTAC 6 cut(s) 7, 536, 631, 826, 955, 970
DdeI CTNAG 3 cut(s) 560, 830, 966
DpnI GATC 3 cut(s) 192, 419, 704
DpnII GATC 3 cut(s) 190, 417, 702
DrdI GACNNNNNNGTC 1 cut(s) 858
DseDI GACNNNNNNGTC 1 cut(s) 858
Eam1104I CTCTTC 3 cut(s) 96, 387, 573
EarI CTCTTC 3 cut(s) 96, 387, 573
Eco31I GGTCTC 2 cut(s) 570, 781
Eco32I GATATC 1 cut(s) 55
Eco57I CTGAAG 3 cut(s) 302, 594, 1036
Eco81I CCTNAGG 1 cut(s) 560
Eco88I CYCGRG 2 cut(s) 936, 997
EcoRI GAATTC 1 cut(s) 478
EcoRII CCWGG 1 cut(s) 633
EcoRV GATATC 1 cut(s) 55
Esp3I CGTCTC 1 cut(s) 937
FaeI CATG 4 cut(s) 77, 670, 815, 964
FaiI YATR 9 cut(s) 75, 218, 668, 773, 813, 903, 962, 1005, 1007
FalI AAGNNNNNCTT 4 cut(s) 93, 125, 479, 511
FaqI GGGAC 2 cut(s) 271, 655
FatI CATG 4 cut(s) 73, 666, 811, 960
FauI CCCGC 1 cut(s) 525
Fnu4HI GCNGC 4 cut(s) 20, 32, 35, 38
FokI GGATG 3 cut(s) 61, 145, 741
Fsp4HI GCNGC 4 cut(s) 20, 32, 35, 38
FspBI CTAG 1 cut(s) 539
GluI GCNGC 4 cut(s) 20, 32, 35, 38
GsuI CTGGAG 3 cut(s) 194, 728, 768
HaeIII GGCC 3 cut(s) 660, 920, 987
HapII CCGG 1 cut(s) 716
HgaI GACGC 1 cut(s) 919
Hin1I GRCGYC 1 cut(s) 687
Hin1II CATG 4 cut(s) 77, 670, 815, 964
HindIII AAGCTT 2 cut(s) 185, 488
HinfI GANTC 6 cut(s) 224, 325, 353, 444, 719, 859
HpaII CCGG 1 cut(s) 716
HphI GGTGA 4 cut(s) 505, 697, 829, 907
Hpy166II GTNNAC 2 cut(s) 268, 851
Hpy188I TCNGA 1 cut(s) 728
Hpy8I GTNNAC 2 cut(s) 268, 851
Hpy99I CGWCG 2 cut(s) 266, 1029
HpyAV CCTTC 6 cut(s) 58, 299, 356, 755, 919, 944
HpyCH4IV ACGT 3 cut(s) 249, 264, 687
HpyCH4V TGCA 4 cut(s) 183, 268, 381, 811
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 28, 37, 555, 748, 808
HpyF3I CTNAG 3 cut(s) 560, 830, 966
HpySE526I ACGT 3 cut(s) 249, 264, 687
Hsp92I GRCGYC 1 cut(s) 687
Hsp92II CATG 4 cut(s) 77, 670, 815, 964
Kzo9I GATC 3 cut(s) 190, 417, 702
LmnI GCTCC 3 cut(s) 432, 747, 980
Lsp1109I GCAGC 3 cut(s) 31, 43, 49
LweI GCATC 2 cut(s) 133, 532
MaeI CTAG 1 cut(s) 539
MaeII ACGT 3 cut(s) 249, 264, 687
MaeIII GTNAC 2 cut(s) 240, 384
MalI GATC 3 cut(s) 192, 419, 704
MbiI CCGCTC 1 cut(s) 975
MboI GATC 3 cut(s) 190, 417, 702
MboII GAAGA 8 cut(s) 73, 110, 113, 404, 487, 560, 587, 883
MfeI CAATTG 1 cut(s) 300
MhlI GDGCHC 2 cut(s) 270, 429
MluCI AATT 4 cut(s) 166, 300, 376, 478
MlyI GAGTC 2 cut(s) 713, 868
MmeI TCCRAC 1 cut(s) 507
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 1 cut(s) 716
MspR9I CCNGG 2 cut(s) 635, 716
MunI CAATTG 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 1 cut(s) 635
MwoI GCNNNNNNNGC 6 cut(s) 19, 28, 37, 555, 748, 808
NciI CCSGG 1 cut(s) 716
NdeII GATC 3 cut(s) 190, 417, 702
NlaIII CATG 4 cut(s) 77, 670, 815, 964
NlaIV GGNNCC 3 cut(s) 767, 907, 919
NmuCI GTSAC 1 cut(s) 384
NspI RCATGY 1 cut(s) 815
NspV TTCGAA 1 cut(s) 447
PaeI GCATGC 1 cut(s) 815
PaeR7I CTCGAG 1 cut(s) 936
PagI TCATGA 1 cut(s) 73
PcsI WCGNNNNNNNCGW 2 cut(s) 684, 795
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 4 cut(s) 224, 325, 353, 444
PflMI CCANNNNNTGG 1 cut(s) 634
PfoI TCCNGGA 1 cut(s) 714
PkrI GCNGC 4 cut(s) 21, 33, 36, 39
PleI GAGTC 2 cut(s) 713, 867
PpsI GAGTC 2 cut(s) 713, 867
Psp6I CCWGG 1 cut(s) 633
PspGI CCWGG 1 cut(s) 633
PspN4I GGNNCC 3 cut(s) 767, 907, 919
PspPI GGNCC 1 cut(s) 918
PstNI CAGNNNCTG 1 cut(s) 634
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 6 cut(s) 8, 537, 632, 827, 956, 971
RsaNI GTAC 6 cut(s) 7, 536, 631, 826, 955, 970
SatI GCNGC 4 cut(s) 20, 32, 35, 38
Sau3AI GATC 3 cut(s) 190, 417, 702
Sau96I GGNCC 1 cut(s) 918
SchI GAGTC 2 cut(s) 713, 868
ScrFI CCNGG 2 cut(s) 635, 716
SduI GDGCHC 2 cut(s) 270, 429
SfaNI GCATC 2 cut(s) 133, 532
Sfr274I CTCGAG 1 cut(s) 936
SfuI TTCGAA 1 cut(s) 447
SlaI CTCGAG 1 cut(s) 936
SmlI CTYRAG 6 cut(s) 110, 320, 461, 581, 734, 936
SmoI CTYRAG 6 cut(s) 110, 320, 461, 581, 734, 936
SphI GCATGC 1 cut(s) 815
Sse9I AATT 4 cut(s) 166, 300, 376, 478
SsiI CCGC 5 cut(s) 35, 532, 708, 815, 973
SspMI CTAG 1 cut(s) 539
StyD4I CCNGG 2 cut(s) 633, 714
TaiI ACGT 3 cut(s) 252, 267, 690
TaqII GACCGA 1 cut(s) 110
TasI AATT 4 cut(s) 166, 300, 376, 478
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 4 cut(s) 224, 325, 353, 444
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 384
TseI GCWGC 3 cut(s) 19, 31, 37
Tsp45I GTSAC 1 cut(s) 384
TspDTI ATGAA 1 cut(s) 857
TspGWI ACGGA 1 cut(s) 463
TspRI CASTG 1 cut(s) 393
Van91I CCANNNNNTGG 1 cut(s) 634
VneI GTGCAC 1 cut(s) 266
XapI RAATTY 1 cut(s) 478
XceI RCATGY 1 cut(s) 815
XhoI CTCGAG 1 cut(s) 936
XspI CTAG 1 cut(s) 539
ZraI GACGTC 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.