MD16G1245200.v1.1

Plant intracellular ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014334
Physical Location & Seq
Reverse (-)
26705118 .. 26709545
4428 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1245200.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGATGTACGAGCAGCAGCAGCAGCAAATGAGAATGGACACGAAGAAAAAAGCTGTTCATGAGAGGAAGAGATCGATAGATGAAGAGAGGCTTGAGATAGTGGATTTGAGTGGGATGTGTCTTGATGCCCTTCCCAATCCTTCCCTTAATTTGGGGGCTATTTGCAAGCTTGATCTTTCCAATAACAACCTTCAGAACATACCAGAATCCTTAACAGCAAGATTACTGAACGTGGTAGTGCTGGACGTGCATTCAAATCAGCTGAAATCTCTTCCAAACTCAATTGGATGTTTGTCCAAGCTTAAGGTTCTCAATGTTTCCGGAAACCTTCTTTCTTATCTCCCAAACACTATCGAAAATTGCAGGTCAATAGAAGAGCTGAATGCCAACTTCAACAAGCTGAGCCACCTCCCAGACACCATTGGTTTTGAACTTAATAACCTCAAGAAGCTATCAGTGAACTCCAACAAGCTAATCTTTCTACCACGCTCCCTCACCCACCTTACGTCTCTGCGTATACTCGATGCTCGTCTCAACTGCCTCAGGTCCCTTCCCGAAGATCTCGAGAACCTTATCAACCTCGAAGTTCTCAATGTGAGCCAAAACTTCCAGTACCTCGAAATCCTACCATACTCCATTGGCCTCCTCATGTCTCTTGTTGAATTGGACGTCAGCTACAACAAGATCACAGCCCTACCAGACTCCATTGGATGCCTCAAGAAGCTTCAGAAGCTTACTGTGGAAGGAAACCCTATGGTTTCTCCTCCTCCTGAGGTGTTCGAGCAAGGGTTGCATTCAGTGAAGGAGTACCTTAGTGAGAAAATGAATGGCGGCCACAAGACTCCTCCCAAGAAGAAGTCTTGGATGGGCAAATTGGTGAAGTATGGCACGTTCAACGGGGCCAACCGAAGCAGACCGCGAGAGGAGAGAGAAGGGTTCATCATGCCTGAGTACCGGTCATCAATTGACGGCCTTGCTTCACCGAGGTATATGGGGATGTTCTCACCTCGTCGCCTCTTCTCTCCCCGTACTTACTTCACTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8

Protein Analysis

349

Amino Acids

39.5

Weight (kDa)

9.09

Isoelectric Point (pI)

52.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 56 - 111 2.3e-08 Leucine rich repeat
LRR_14 PF23598 57 - 152 1.5e-08 Leucine-rich repeat region
LRR_8 PF13855 123 - 177 1e-05 Leucine rich repeat
LRR_8 PF13855 193 - 251 1.2e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014334)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19330 AT4G29880
fragaria_vesca FvH4_4g06910
malus_domestica MD13G1241200.v1.1 MD16G1245200.v1.1
prunus_persica Prupe.1G072100_v2.0.a1
pyrus_communis pycom13g21190
rosa_chinensis RchiOBHm_Chr4g0398811
rosa_laevigata RLG00000009317
rosa_multiflora Rmu_sc0004751.1_g000026
rosa_roxburghii Rroxscaffold_5G00343770
rosa_rugosa Rorug04G0012000
rosa_samantha Rh4AG089700 Rh4BG087100 Rh4CG097700 Rh4DG081400
rosa_wichuraiana Rw4G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 672
Acc36I ACCTGC 1 cut(s) 354
AccI GTMKAC 1 cut(s) 517
AccII CGCG 1 cut(s) 919
AccIII TCCGGA 1 cut(s) 320
AciI CCGC 2 cut(s) 831, 917
AcoI YGGCCR 1 cut(s) 832
AcuI CTGAAG 2 cut(s) 176, 710
AcyI GRCGYC 1 cut(s) 669
AfaI GTAC 5 cut(s) 8, 614, 809, 953, 1030
AfiI CCNNNNNNNGG 1 cut(s) 151
AflII CTTAAG 1 cut(s) 302
AgeI ACCGGT 1 cut(s) 954
AgsI TTSAA 5 cut(s) 255, 394, 431, 662, 895
AjiI CACGTC 1 cut(s) 247
Alw26I GTCTC 3 cut(s) 513, 536, 657
Ama87I CYCGRG 1 cut(s) 563
Aor13HI TCCGGA 1 cut(s) 320
AoxI GGCC 4 cut(s) 640, 832, 900, 970
ApeKI GCWGC 4 cut(s) 13, 16, 19, 22
AsiGI ACCGGT 1 cut(s) 954
AspS9I GGNCC 2 cut(s) 546, 900
AsuHPI GGTGA 4 cut(s) 487, 889, 972, 996
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 546
AxyI CCTNAGG 2 cut(s) 542, 771
BbvI GCAGC 4 cut(s) 25, 28, 31, 34
BccI CCATC 1 cut(s) 859
BceAI ACGGC 1 cut(s) 985
BcoDI GTCTC 3 cut(s) 513, 536, 657
BfrI CTTAAG 1 cut(s) 302
BfuAI ACCTGC 1 cut(s) 354
BglII AGATCT 1 cut(s) 559
BisI GCNGC 5 cut(s) 14, 17, 20, 23, 832
BlpI GCTNAGC 1 cut(s) 401
BlsI GCNGC 5 cut(s) 15, 18, 21, 24, 833
Bme18I GGWCC 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 563
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 2 cut(s) 546, 900
BmiI GGNNCC 2 cut(s) 548, 901
BmsI GCATC 3 cut(s) 115, 514, 701
Bpu1102I GCTNAGC 1 cut(s) 401
BpuEI CTTGAG 3 cut(s) 113, 428, 701
Bsa29I ATCGAT 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 669
BsaJI CCNNGG 1 cut(s) 983
BsaWI WCCGGW 2 cut(s) 320, 954
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse118I RCCGGY 1 cut(s) 954
Bse1I ACTGG 1 cut(s) 610
Bse21I CCTNAGG 2 cut(s) 542, 771
BseAI TCCGGA 1 cut(s) 320
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 1 cut(s) 983
BseGI GGATG 5 cut(s) 120, 293, 716, 870, 1002
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMII CTCAG 4 cut(s) 392, 556, 762, 939
BseNI ACTGG 1 cut(s) 610
BseRI GAGGAG 5 cut(s) 635, 753, 756, 834, 938
BseXI GCAGC 4 cut(s) 25, 28, 31, 34
Bsh1236I CGCG 1 cut(s) 919
BshFI GGCC 4 cut(s) 642, 834, 902, 972
BshTI ACCGGT 1 cut(s) 954
BshVI ATCGAT 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 2 cut(s) 321, 955
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 1 cut(s) 151
BsmAI GTCTC 3 cut(s) 513, 536, 657
BsmBI CGTCTC 2 cut(s) 513, 536
BsmFI GGGAC 1 cut(s) 532
BsmI GAATGC 3 cut(s) 250, 388, 793
BsnI GGCC 4 cut(s) 642, 834, 902, 972
BsoBI CYCGRG 1 cut(s) 563
Bsp13I TCCGGA 1 cut(s) 320
Bsp143I GATC 4 cut(s) 71, 172, 559, 684
Bsp1720I GCTNAGC 1 cut(s) 401
BspACI CCGC 2 cut(s) 831, 917
BspANI GGCC 4 cut(s) 642, 834, 902, 972
BspCNI CTCAG 4 cut(s) 393, 555, 763, 940
BspDI ATCGAT 1 cut(s) 74
BspEI TCCGGA 1 cut(s) 320
BspFNI CGCG 1 cut(s) 919
BspHI TCATGA 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 548, 901
BspMI ACCTGC 1 cut(s) 354
BspQI GCTCTTC 1 cut(s) 369
BspTI CTTAAG 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 954
BsrI ACTGG 1 cut(s) 610
BssAI RCCGGY 1 cut(s) 954
BssECI CCNNGG 1 cut(s) 983
BssMI GATC 4 cut(s) 71, 172, 559, 684
BssNAI GTATAC 1 cut(s) 518
BssNI GRCGYC 1 cut(s) 669
Bst1107I GTATAC 1 cut(s) 518
Bst4CI ACNGT 1 cut(s) 739
Bst6I CTCTTC 5 cut(s) 62, 78, 276, 369, 1022
BstACI GRCGYC 1 cut(s) 669
BstAFI CTTAAG 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 790
BstC8I GCNNGC 1 cut(s) 167
BstDEI CTNAG 5 cut(s) 401, 542, 771, 812, 948
BstF5I GGATG 5 cut(s) 120, 293, 716, 870, 1002
BstFNI CGCG 1 cut(s) 919
BstKTI GATC 4 cut(s) 74, 175, 562, 687
BstMAI GTCTC 3 cut(s) 513, 536, 657
BstMBI GATC 4 cut(s) 71, 172, 559, 684
BstMWI GCNNNNNNNGC 5 cut(s) 19, 22, 247, 730, 790
BstUI CGCG 1 cut(s) 919
BstV1I GCAGC 4 cut(s) 25, 28, 31, 34
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BstZ17I GTATAC 1 cut(s) 518
Bsu15I ATCGAT 1 cut(s) 74
Bsu36I CCTNAGG 2 cut(s) 542, 771
BsuRI GGCC 4 cut(s) 642, 834, 902, 972
BsuTUI ATCGAT 1 cut(s) 74
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 5 cut(s) 120, 293, 716, 870, 1002
BtsIMutI CAGTG 2 cut(s) 462, 804
BveI ACCTGC 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 167
CciI TCATGA 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 954
Cfr13I GGNCC 2 cut(s) 546, 900
ClaI ATCGAT 1 cut(s) 74
Csp6I GTAC 5 cut(s) 7, 613, 808, 952, 1029
CspAI ACCGGT 1 cut(s) 954
CviAII CATG 3 cut(s) 59, 649, 943
CviQI GTAC 5 cut(s) 7, 613, 808, 952, 1029
DdeI CTNAG 5 cut(s) 401, 542, 771, 812, 948
DpnI GATC 4 cut(s) 73, 174, 561, 686
DpnII GATC 4 cut(s) 71, 172, 559, 684
EaeI YGGCCR 1 cut(s) 832
Eam1104I CTCTTC 5 cut(s) 62, 78, 276, 369, 1022
EarI CTCTTC 5 cut(s) 62, 78, 276, 369, 1022
Eco47I GGWCC 1 cut(s) 546
Eco57I CTGAAG 2 cut(s) 176, 710
Eco81I CCTNAGG 2 cut(s) 542, 771
Eco88I CYCGRG 1 cut(s) 563
EcoO109I RGGNCCY 1 cut(s) 546
Esp3I CGTCTC 2 cut(s) 513, 536
FaeI CATG 3 cut(s) 62, 652, 946
FalI AAGNNNNNCTT 4 cut(s) 75, 107, 461, 493
FaqI GGGAC 1 cut(s) 532
FatI CATG 3 cut(s) 58, 648, 942
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 5 cut(s) 14, 17, 20, 23, 832
FokI GGATG 5 cut(s) 127, 300, 723, 877, 1009
Fsp4HI GCNGC 5 cut(s) 14, 17, 20, 23, 832
GluI GCNGC 5 cut(s) 14, 17, 20, 23, 832
HaeIII GGCC 4 cut(s) 642, 834, 902, 972
HapII CCGG 2 cut(s) 321, 955
Hin1I GRCGYC 1 cut(s) 669
Hin1II CATG 3 cut(s) 62, 652, 946
HindIII AAGCTT 4 cut(s) 167, 299, 722, 731
HinfI GANTC 3 cut(s) 206, 701, 841
HpaII CCGG 2 cut(s) 321, 955
HphI GGTGA 4 cut(s) 487, 889, 972, 996
Hpy166II GTNNAC 2 cut(s) 460, 518
Hpy188I TCNGA 2 cut(s) 195, 729
Hpy188III TCNNGA 9 cut(s) 59, 122, 321, 445, 554, 563, 565, 718, 770
Hpy8I GTNNAC 2 cut(s) 460, 518
Hpy99I CGWCG 1 cut(s) 1014
HpyAV CCTTC 8 cut(s) 140, 150, 200, 338, 560, 737, 796, 926
HpyCH4III ACNGT 1 cut(s) 739
HpyCH4IV ACGT 5 cut(s) 231, 246, 506, 669, 890
HpyCH4V TGCA 4 cut(s) 165, 250, 363, 793
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 22, 247, 730, 790
HpyF3I CTNAG 5 cut(s) 401, 542, 771, 812, 948
HpySE526I ACGT 5 cut(s) 231, 246, 506, 669, 890
Hsp92I GRCGYC 1 cut(s) 669
Hsp92II CATG 3 cut(s) 62, 652, 946
Kpn2I TCCGGA 1 cut(s) 320
Kzo9I GATC 4 cut(s) 71, 172, 559, 684
LguI GCTCTTC 1 cut(s) 369
LmnI GCTCC 1 cut(s) 494
Lsp1109I GCAGC 4 cut(s) 25, 28, 31, 34
LweI GCATC 3 cut(s) 115, 514, 701
MaeII ACGT 5 cut(s) 231, 246, 506, 669, 890
MalI GATC 4 cut(s) 73, 174, 561, 686
MboI GATC 4 cut(s) 71, 172, 559, 684
MboII GAAGA 8 cut(s) 55, 79, 95, 263, 386, 569, 865, 1009
MfeI CAATTG 2 cut(s) 282, 963
MflI RGATCY 1 cut(s) 559
MluCI AATT 6 cut(s) 148, 282, 358, 662, 872, 963
MlyI GAGTC 2 cut(s) 695, 835
MmeI TCCRAC 1 cut(s) 489
MroI TCCGGA 1 cut(s) 320
MseI TTAA 4 cut(s) 147, 212, 303, 435
MspA1I CMGCKG 1 cut(s) 262
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 2 cut(s) 321, 955
MunI CAATTG 2 cut(s) 282, 963
Mva1269I GAATGC 3 cut(s) 250, 388, 793
MvnI CGCG 1 cut(s) 919
MwoI GCNNNNNNNGC 5 cut(s) 19, 22, 247, 730, 790
NdeII GATC 4 cut(s) 71, 172, 559, 684
NlaIII CATG 3 cut(s) 62, 652, 946
NlaIV GGNNCC 2 cut(s) 548, 901
PaeR7I CTCGAG 1 cut(s) 563
PagI TCATGA 1 cut(s) 58
PciSI GCTCTTC 1 cut(s) 369
PctI GAATGC 3 cut(s) 250, 388, 793
PfeI GAWTC 1 cut(s) 206
PinAI ACCGGT 1 cut(s) 954
PkrI GCNGC 5 cut(s) 15, 18, 21, 24, 833
PleI GAGTC 2 cut(s) 695, 835
PpsI GAGTC 2 cut(s) 695, 835
PpuMI RGGWCCY 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 546
PspN4I GGNNCC 2 cut(s) 548, 901
PspPI GGNCC 2 cut(s) 546, 900
PspPPI RGGWCCY 1 cut(s) 546
PsrI GAACNNNNNNTAC 2 cut(s) 875, 907
PsuI RGATCY 1 cut(s) 559
PvuII CAGCTG 1 cut(s) 262
RsaI GTAC 5 cut(s) 8, 614, 809, 953, 1030
RsaNI GTAC 5 cut(s) 7, 613, 808, 952, 1029
SapI GCTCTTC 1 cut(s) 369
SaqAI TTAA 4 cut(s) 147, 212, 303, 435
SatI GCNGC 5 cut(s) 14, 17, 20, 23, 832
Sau3AI GATC 4 cut(s) 71, 172, 559, 684
Sau96I GGNCC 2 cut(s) 546, 900
SchI GAGTC 2 cut(s) 695, 835
SfaNI GCATC 3 cut(s) 115, 514, 701
Sfr274I CTCGAG 1 cut(s) 563
SinI GGWCC 1 cut(s) 546
SlaI CTCGAG 1 cut(s) 563
SmlI CTYRAG 5 cut(s) 92, 302, 443, 563, 716
SmoI CTYRAG 5 cut(s) 92, 302, 443, 563, 716
Sse9I AATT 6 cut(s) 148, 282, 358, 662, 872, 963
SsiI CCGC 2 cut(s) 831, 917
TaaI ACNGT 1 cut(s) 739
TaiI ACGT 5 cut(s) 234, 249, 509, 672, 893
TaqI TCGA 7 cut(s) 74, 354, 522, 564, 582, 618, 780
TasI AATT 6 cut(s) 148, 282, 358, 662, 872, 963
TauI GCSGC 1 cut(s) 834
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 4 cut(s) 147, 212, 303, 435
Tru9I TTAA 4 cut(s) 147, 212, 303, 435
TscAI CASTG 2 cut(s) 462, 804
TseI GCWGC 4 cut(s) 13, 16, 19, 22
TspDTI ATGAA 4 cut(s) 47, 96, 839, 928
TspRI CASTG 2 cut(s) 462, 804
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 546
XhoI CTCGAG 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 517
ZraI GACGTC 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.