MD13G1251600.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009414
Physical Location & Seq
Reverse (-)
26783984 .. 26793489
9506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1251600.v1.1.491

Sequence Viewer

Length: 2394 bp
ATGGTTAGGGGCGGCAAGAGCTACGTGTCAGCACCTCCTGCCTTCTCCGCTGACGCCAAGCGCCTCCTCGTCTGCACAGGCACCGCCGTCTCCATCTTTAGCACCTCCACCGGTTTGCAGATATCGTCCTTGGAGGGTCACAATGGCTTGGTCACCTCCGTCATAGTAGTTCCGGGCAACAAAGCTCTGAGTTTCTGCTGGACCGCCTCCGTCGACGGCACCATTCGCTATTGGGACTTTGCTGTTCCTGAGTTGATGAAGGCCATTGATATCAAAATGCCCATTTTCTCTATGGTTATTCCGTCCATAATGTTCCAACCCGGGAAAGGAGCACGAGAGGATGATTCTAATGCAGCTCATGTTTTTGCTTATTTGTCTGTTGAAAATACCCAAGTGCAACAAAACACACCAAAGCCGTTGCGTGGACAGATTCGGAAGTGTAATTTGACCGAATCTCGTTTGGCTGGCGGTGCTCTCTCTGAGACTCGAAAACCAGAGGCTATAACTATCAGTCCCTCAGGAAAATTCTTTGGCATCCGGAACAAACGGAAGCTTCATATATGGAGAGTCCCTGCTATAGACTCGAAACTCATGATTGCTAAGAAGATTACTCTGCATCATACAAGAAACTTTAACGTTCTTGCATTTCATCCAACTCAGGCAATTGTTGCCGCAGGTGATACAAGTGGGAGAATTCTAATCTGGACAGGATTCGGTAAACGTACATTTTCCGATGGTGATAAATTAATGAAAGGAAGATCAATGAATAATGAGGAAGAAAGAGATGGAGTTAGGGGAGATGATGATGCTGATGCTTCCTCCACATGGCATTGGCATGCGGCTGGAATAAGTGTCCTTTCTTTCTCTTCTGATGGTGCCTATTTATATTCAGGTGGAATAGAAGGAGTTCTTGTGGTCTGGCAGCTAGACACAGGGAAAAAGAAGTTCTTGCCTAGAATTGGATCTCCTCTTCTATATTTGACTGATTCTCCAGACCCTTCACTTTCTTCTATATCTTGTGCAGATAATCAAATTCATATACTAAAAATGCCCTCCATGGAAATCATGAAGTCTATTTCAGGGATCAAGCTTCCTTATTCATGTCCAGAGATATATGATGGAATATGCAGCAAGTTTTCCTTTGATCACACTGATGGGTTGGTTGCCTTGCGTACCGCGAATTATTGCATCCAGTTCTACAGCTTGTTTGATGACCGTGAAATGTCTGAGGTTCAAATATGTGAAAGAAATCATCAACCAGGGGATGAAGTCAGAGTAATAGTGACTTTGGTGTCTCTATCTAAAGATGGCTCTATGATGAGCACTGTTGAAGTTAAGCTTCCTGAGGAGGGGTTAGGTGGTCTTGTTTGTCTTAAATTTTGGGCATCAGGGCCACAGAACAAAAATTTCAGCCTGTCCACCGTTGTTTATGAACCTCACAGGGATGCTAGAATTTCTTCTCTCGCGTTCCATCCTACTCGTCAAATGGTTGCTAGCTTATCATATGGTGGGGATTTCAAGATTTGGGTTTGCAGTGATGAGATTCAGCCGAAGCATGAGGTGCTGCAACATTCTGGGTGGATGTGTCATGCCGTAGGTTCATACAAGAATAAGCTAATGACAGCTGTTGCATTTTCTGGCGATGGTTCTGTTCTGGCGGTTGCAGCTGAAAACATAATCACTCTATGGGACCCCAACAACAATGCACTTGTAGCTGTCATTGGAGAGATGCAGATGCCAATTGCAACCCTATCTTTTGCTGGGAAATCAGAGTATCTCGTGTCTGTCACTCAAGGTTCAAAACCGCAACTATCGGTGTGGAGCATGTCAGAACTATCTGAATCTTGGTCATATAAGCTACATGTAGAAGCTGTTACCTGTGCGGTAGATTCGTCATCATTTGCAGTTCTATCTTTACTAGGAAAAGATGGGGTAATCTTATTATTCAATGCAACAGATCCTGTTCCATTGGCTATGTGGTCTGTAAGGAAGGCTCAGGGGGGCGGGCTTGCTTTTCTCAACAGCGACCAACTTTCTAACCAACAAAATATTTTAGATAGGAACCGTCCACAAGCTTTGCTTGTCTATATAAATGGTGACCATGAATATGTCATCTTTGACCCATATAACATTGAAGCAAAAGAACTTAGTCTAACCAGGCCGGGGAGTCATGTTGCCCCTGATGAAACAGGGCATTTTGGATATTCATCTATCTACGGGGAGCTACCGAAGTTTGACATAAAGAGAGACCAGACCGTGGCCCCATCTGCTCCATCACAGAGGCCTTGGGAGACCATATTTAGTGGGTCATCTCATGAACTTCCACCCCTAACCAAACTGTGTTCAGATTTTATGGAATCACTGCTAGAAAGGAGGACTGCAATGGTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
WD40

Protein Analysis

798

Amino Acids

87.45

Weight (kDa)

6.33

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR75_1st PF23869 15 - 241 6.5e-19 WD repeat-containing protein 75 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 185 - 367 4.3e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR75_1st PF23869 273 - 343 7.2e-15 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR75_2nd PF23769 396 - 659 3.8e-36 WD repeat-containing protein 75 second beta-propeller
WD40_Prp19 PF24814 474 - 625 1.2e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 481 - 622 1.5e-09 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 482 - 610 1.2e-07 WDHD1 first WD40 domain
WDR55 PF24796 482 - 652 6.1e-06 WDR55
Beta-prop_WDR5 PF25175 537 - 629 3.4e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 665
AasI GACNNNNNNGTC 1 cut(s) 1291
Acc36I ACCTGC 1 cut(s) 665
AccB1I GGYRCC 3 cut(s) 80, 218, 875
AccB7I CCANNNNNTGG 1 cut(s) 2257
AccI GTMKAC 1 cut(s) 213
AccII CGCG 2 cut(s) 1178, 1466
AccIII TCCGGA 1 cut(s) 537
AclI AACGTT 1 cut(s) 636
AclWI GGATC 3 cut(s) 970, 1091, 1952
AcsI RAATTY 6 cut(s) 524, 693, 1032, 1376, 1405, 1452
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 724, 1174
AfiI CCNNNNNNNGG 7 cut(s) 326, 422, 825, 959, 1349, 2163, 2257
AflIII ACRYGT 2 cut(s) 24, 1861
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 7 cut(s) 383, 1235, 1331, 1519, 1800, 1948, 2135
AjnI CCWGG 2 cut(s) 1258, 2156
Alw21I GWGCWC 3 cut(s) 334, 475, 1325
Alw26I GTCTC 5 cut(s) 94, 476, 1299, 2241, 2285
AlwI GGATC 3 cut(s) 970, 1091, 1952
AlwNI CAGNNNCTG 1 cut(s) 1961
Ama87I CYCGRG 1 cut(s) 320
Aor13HI TCCGGA 1 cut(s) 537
AoxI GGCC 5 cut(s) 261, 1391, 2159, 2259, 2282
ApeKI GCWGC 5 cut(s) 353, 922, 1128, 1564, 1664
ApoI RAATTY 6 cut(s) 524, 693, 1032, 1376, 1405, 1452
AseI ATTAAT 1 cut(s) 746
AsiGI ACCGGT 1 cut(s) 110
Asp700I GAANNNNTTC 2 cut(s) 906, 1456
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 4 cut(s) 201, 1391, 1690, 2260
AsuC2I CCSGG 4 cut(s) 174, 321, 322, 2163
AsuHPI GGTGA 4 cut(s) 145, 689, 749, 2108
AsuNHI GCTAGC 1 cut(s) 1493
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 2 cut(s) 201, 1690
AxyI CCTNAGG 2 cut(s) 517, 1344
BanI GGYRCC 3 cut(s) 80, 218, 875
BauI CACGAG 2 cut(s) 333, 1778
Bbv12I GWGCWC 3 cut(s) 334, 475, 1325
BbvI GCAGC 5 cut(s) 365, 934, 1140, 1551, 1676
BceAI ACGGC 4 cut(s) 71, 232, 400, 1577
BciT130I CCWGG 2 cut(s) 1260, 2158
BclI TGATCA 1 cut(s) 1144
BcnI CCSGG 4 cut(s) 174, 321, 322, 2163
BcoDI GTCTC 5 cut(s) 94, 476, 1299, 2241, 2285
BfaI CTAG 6 cut(s) 926, 954, 1449, 1494, 1919, 2366
BfmI CTRYAG 2 cut(s) 576, 1198
BfoI RGCGCY 1 cut(s) 64
BfuAI ACCTGC 1 cut(s) 665
BisI GCNGC 8 cut(s) 13, 354, 672, 840, 923, 1129, 1565, 1665
BlsI GCNGC 8 cut(s) 14, 355, 673, 841, 924, 1130, 1566, 1666
Bme1390I CCNGG 6 cut(s) 174, 321, 322, 1260, 2158, 2163
Bme18I GGWCC 2 cut(s) 201, 1690
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 4 cut(s) 201, 1391, 1690, 2260
BmiI GGNNCC 7 cut(s) 82, 220, 877, 1691, 1692, 2063, 2262
BmrFI CCNGG 6 cut(s) 174, 321, 322, 1260, 2158, 2163
BmsI GCATC 9 cut(s) 543, 625, 796, 802, 1197, 1394, 1435, 1719, 1725
BmtI GCTAGC 1 cut(s) 1497
BpmI CTGGAG 1 cut(s) 975
Bpu10I CCTNAGC 1 cut(s) 1995
BpuEI CTTGAG 1 cut(s) 1776
BpuMI CCSGG 4 cut(s) 174, 321, 322, 2163
BsaAI YACGTR 1 cut(s) 25
BsaBI GATNNNNATC 1 cut(s) 2206
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 2 cut(s) 2241, 2285
BsaJI CCNNGG 7 cut(s) 129, 320, 1056, 1259, 2162, 2256, 2285
BsaWI WCCGGW 2 cut(s) 110, 537
BsaXI ACNNNNNCTCC 4 cut(s) 897, 927, 973, 1003
Bsc4I CCNNNNNNNGG 7 cut(s) 326, 422, 825, 959, 1349, 2163, 2257
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 1 cut(s) 1192
Bse21I CCTNAGG 2 cut(s) 517, 1344
Bse3DI GCAATG 1 cut(s) 2388
Bse8I GATNNNNATC 1 cut(s) 2206
BseAI TCCGGA 1 cut(s) 537
BseBI CCWGG 2 cut(s) 1260, 2158
BseDI CCNNGG 7 cut(s) 129, 320, 1056, 1259, 2162, 2256, 2285
BseGI GGATG 8 cut(s) 346, 534, 649, 1188, 1270, 1450, 1471, 1587
BseJI GATNNNNATC 1 cut(s) 2206
BseLI CCNNNNNNNGG 7 cut(s) 326, 422, 825, 959, 1349, 2163, 2257
BseMI GCAATG 1 cut(s) 2388
BseMII CTCAG 8 cut(s) 179, 240, 471, 531, 671, 1218, 1335, 2009
BseNI ACTGG 1 cut(s) 1192
BseRI GAGGAG 3 cut(s) 56, 957, 1361
BseXI GCAGC 5 cut(s) 365, 934, 1140, 1551, 1676
BseYI CCCAGC 1 cut(s) 1760
BsgI GTGCAG 2 cut(s) 58, 1041
Bsh1236I CGCG 2 cut(s) 1178, 1466
BshFI GGCC 5 cut(s) 263, 1393, 2161, 2261, 2284
BshNI GGYRCC 3 cut(s) 80, 218, 875
BshTI ACCGGT 1 cut(s) 110
BsiHKAI GWGCWC 3 cut(s) 334, 475, 1325
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 5 cut(s) 111, 173, 321, 538, 2162
BslFI GGGAC 4 cut(s) 248, 498, 554, 1703
BslI CCNNNNNNNGG 7 cut(s) 326, 422, 825, 959, 1349, 2163, 2257
BsmAI GTCTC 5 cut(s) 94, 476, 1299, 2241, 2285
BsmBI CGTCTC 1 cut(s) 94
BsmFI GGGAC 4 cut(s) 248, 498, 554, 1703
BsnI GGCC 5 cut(s) 263, 1393, 2161, 2261, 2284
Bso31I GGTCTC 2 cut(s) 2241, 2285
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 3 cut(s) 334, 475, 1325
Bsp13I TCCGGA 1 cut(s) 537
Bsp143I GATC 5 cut(s) 758, 962, 1083, 1144, 1957
Bsp19I CCATGG 1 cut(s) 1056
BspANI GGCC 5 cut(s) 263, 1393, 2161, 2261, 2284
BspCNI CTCAG 8 cut(s) 180, 241, 472, 530, 670, 1219, 1336, 2008
BspEI TCCGGA 1 cut(s) 537
BspFNI CGCG 2 cut(s) 1178, 1466
BspHI TCATGA 3 cut(s) 591, 1065, 2314
BspLI GGNNCC 7 cut(s) 82, 220, 877, 1691, 1692, 2063, 2262
BspMI ACCTGC 1 cut(s) 665
BspOI GCTAGC 1 cut(s) 1497
BspPI GGATC 3 cut(s) 970, 1091, 1952
BspT107I GGYRCC 3 cut(s) 80, 218, 875
BspTNI GGTCTC 2 cut(s) 2241, 2285
BsrDI GCAATG 1 cut(s) 2388
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 1 cut(s) 1192
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 7 cut(s) 129, 320, 1056, 1259, 2162, 2256, 2285
BssMI GATC 5 cut(s) 758, 962, 1083, 1144, 1957
BssNI GRCGYC 1 cut(s) 54
BssSI CACGAG 2 cut(s) 333, 1778
BssT1I CCWWGG 3 cut(s) 129, 1056, 2285
Bst2BI CACGAG 2 cut(s) 333, 1778
Bst2UI CCWGG 2 cut(s) 1260, 2158
Bst4CI ACNGT 6 cut(s) 1217, 1327, 1423, 2066, 2257, 2340
Bst6I CTCTTC 2 cut(s) 871, 975
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 3 cut(s) 38, 668, 1561
BstBAI YACGTR 1 cut(s) 25
BstC8I GCNNGC 5 cut(s) 466, 837, 1495, 2006, 2010
BstDSI CCRYGG 2 cut(s) 1056, 2256
BstEII GGTNACC 2 cut(s) 151, 2096
BstF5I GGATG 8 cut(s) 346, 534, 649, 1188, 1270, 1450, 1471, 1587
BstFNI CGCG 2 cut(s) 1178, 1466
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 5 cut(s) 761, 965, 1086, 1147, 1960
BstMAI GTCTC 5 cut(s) 94, 476, 1299, 2241, 2285
BstMBI GATC 5 cut(s) 758, 962, 1083, 1144, 1957
BstNI CCWGG 2 cut(s) 1260, 2158
BstNSI RCATGY 3 cut(s) 839, 1828, 1865
BstPI GGTNACC 2 cut(s) 151, 2096
BstSCI CCNGG 6 cut(s) 172, 319, 320, 1258, 2156, 2161
BstSFI CTRYAG 2 cut(s) 576, 1198
BstUI CGCG 2 cut(s) 1178, 1466
BstV1I GCAGC 5 cut(s) 365, 934, 1140, 1551, 1676
BstX2I RGATCY 2 cut(s) 962, 1957
BstYI RGATCY 2 cut(s) 962, 1957
Bsu36I CCTNAGG 2 cut(s) 517, 1344
BsuRI GGCC 5 cut(s) 263, 1393, 2161, 2261, 2284
BtgI CCRYGG 2 cut(s) 1056, 2256
BtgZI GCGATG 1 cut(s) 1656
BtsCI GGATG 8 cut(s) 346, 534, 649, 1188, 1270, 1450, 1471, 1587
BtsI GCAGTG 2 cut(s) 1540, 2360
BtsIMutI CAGTG 4 cut(s) 1149, 1323, 1540, 2360
BveI ACCTGC 1 cut(s) 665
Cac8I GCNNGC 5 cut(s) 466, 837, 1495, 2006, 2010
CaiI CAGNNNCTG 1 cut(s) 1961
CciI TCATGA 3 cut(s) 591, 1065, 2314
CfoI GCGC 1 cut(s) 63
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 4 cut(s) 201, 1391, 1690, 2260
Cfr9I CCCGGG 1 cut(s) 320
CseI GACGC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 723, 1173
CspAI ACCGGT 1 cut(s) 110
CspCI CAANNNNNGTGG 2 cut(s) 2058, 2093
CviQI GTAC 2 cut(s) 723, 1173
DpnI GATC 5 cut(s) 760, 964, 1085, 1146, 1959
DpnII GATC 5 cut(s) 758, 962, 1083, 1144, 1957
DrdI GACNNNNNNGTC 1 cut(s) 1291
DseDI GACNNNNNNGTC 1 cut(s) 1291
Eam1104I CTCTTC 2 cut(s) 871, 975
EarI CTCTTC 2 cut(s) 871, 975
Eco130I CCWWGG 3 cut(s) 129, 1056, 2285
Eco147I AGGCCT 1 cut(s) 2284
Eco31I GGTCTC 2 cut(s) 2241, 2285
Eco32I GATATC 2 cut(s) 123, 271
Eco47I GGWCC 2 cut(s) 201, 1690
Eco81I CCTNAGG 2 cut(s) 517, 1344
Eco88I CYCGRG 1 cut(s) 320
Eco91I GGTNACC 2 cut(s) 151, 2096
EcoO109I RGGNCCY 1 cut(s) 1690
EcoO65I GGTNACC 2 cut(s) 151, 2096
EcoRI GAATTC 1 cut(s) 693
EcoRII CCWGG 2 cut(s) 1258, 2156
EcoRV GATATC 2 cut(s) 123, 271
EcoT14I CCWWGG 3 cut(s) 129, 1056, 2285
ErhI CCWWGG 3 cut(s) 129, 1056, 2285
Esp3I CGTCTC 1 cut(s) 94
FaqI GGGAC 4 cut(s) 248, 498, 554, 1703
FauI CCCGC 1 cut(s) 1997
FauNDI CATATG 1 cut(s) 1504
FbaI TGATCA 1 cut(s) 1144
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 8 cut(s) 13, 354, 672, 840, 923, 1129, 1565, 1665
FokI GGATG 8 cut(s) 353, 521, 636, 1175, 1277, 1457, 1458, 1594
Fsp4HI GCNGC 8 cut(s) 13, 354, 672, 840, 923, 1129, 1565, 1665
FspBI CTAG 6 cut(s) 926, 954, 1449, 1494, 1919, 2366
GlaI GCGC 1 cut(s) 62
GluI GCNGC 8 cut(s) 13, 354, 672, 840, 923, 1129, 1565, 1665
GsaI CCCAGC 1 cut(s) 1764
GsuI CTGGAG 1 cut(s) 975
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 5 cut(s) 263, 1393, 2161, 2261, 2284
HapII CCGG 5 cut(s) 111, 173, 321, 538, 2162
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 54
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 4 cut(s) 551, 1088, 1337, 2073
HpaII CCGG 5 cut(s) 111, 173, 321, 538, 2162
HphI GGTGA 4 cut(s) 145, 689, 749, 2108
Hpy166II GTNNAC 5 cut(s) 214, 425, 719, 1419, 2069
Hpy8I GTNNAC 5 cut(s) 214, 425, 719, 1419, 2069
Hpy99I CGWCG 2 cut(s) 215, 218
HpyAV CCTTC 5 cut(s) 52, 253, 896, 1008, 1984
HpyCH4III ACNGT 6 cut(s) 1217, 1327, 1423, 2066, 2257, 2340
HpyCH4IV ACGT 3 cut(s) 24, 636, 721
HpySE526I ACGT 3 cut(s) 24, 636, 721
Hsp92I GRCGYC 1 cut(s) 54
HspAI GCGC 1 cut(s) 61
KflI GGGWCCC 1 cut(s) 1690
Kpn2I TCCGGA 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 1144
Kzo9I GATC 5 cut(s) 758, 962, 1083, 1144, 1957
LmnI GCTCC 4 cut(s) 329, 1821, 2221, 2275
Lsp1109I GCAGC 5 cut(s) 365, 934, 1140, 1551, 1676
LweI GCATC 9 cut(s) 543, 625, 796, 802, 1197, 1394, 1435, 1719, 1725
MaeI CTAG 6 cut(s) 926, 954, 1449, 1494, 1919, 2366
MaeII ACGT 3 cut(s) 24, 636, 721
MaeIII GTNAC 6 cut(s) 137, 151, 1282, 1786, 1873, 2096
MalI GATC 5 cut(s) 760, 964, 1085, 1146, 1959
MboI GATC 5 cut(s) 758, 962, 1083, 1144, 1957
MboII GAAGA 7 cut(s) 616, 768, 788, 858, 962, 999, 1449
MfeI CAATTG 2 cut(s) 663, 1740
MflI RGATCY 2 cut(s) 962, 1957
MhlI GDGCHC 3 cut(s) 334, 475, 1325
MlyI GAGTC 4 cut(s) 478, 575, 576, 2176
MmeI TCCRAC 2 cut(s) 340, 677
MroI TCCGGA 1 cut(s) 537
MroXI GAANNNNTTC 2 cut(s) 906, 1456
MseI TTAA 4 cut(s) 633, 746, 1335, 1374
MslI CAYNNNNRTG 4 cut(s) 834, 1152, 1443, 2106
MspA1I CMGCKG 3 cut(s) 50, 1625, 1667
MspI CCGG 5 cut(s) 111, 173, 321, 538, 2162
MspR9I CCNGG 6 cut(s) 174, 321, 322, 1260, 2158, 2163
MunI CAATTG 2 cut(s) 663, 1740
MvaI CCWGG 2 cut(s) 1260, 2158
MvnI CGCG 2 cut(s) 1178, 1466
NciI CCSGG 4 cut(s) 174, 321, 322, 2163
NcoI CCATGG 1 cut(s) 1056
NdeI CATATG 1 cut(s) 1504
NdeII GATC 5 cut(s) 758, 962, 1083, 1144, 1957
NheI GCTAGC 1 cut(s) 1493
NlaIV GGNNCC 7 cut(s) 82, 220, 877, 1691, 1692, 2063, 2262
NmuCI GTSAC 5 cut(s) 137, 151, 1282, 1786, 2096
NspI RCATGY 3 cut(s) 839, 1828, 1865
PaeI GCATGC 1 cut(s) 839
PagI TCATGA 3 cut(s) 591, 1065, 2314
PaqCI CACCTGC 1 cut(s) 665
PceI AGGCCT 1 cut(s) 2284
PciI ACATGT 1 cut(s) 1861
PdmI GAANNNNTTC 2 cut(s) 906, 1456
PfeI GAWTC 9 cut(s) 344, 430, 452, 711, 986, 1543, 1841, 1889, 2357
PflMI CCANNNNNTGG 1 cut(s) 2257
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 8 cut(s) 14, 355, 673, 841, 924, 1130, 1566, 1666
PleI GAGTC 4 cut(s) 478, 575, 575, 2175
PpsI GAGTC 4 cut(s) 478, 575, 575, 2175
Ppu21I YACGTR 1 cut(s) 25
PpuMI RGGWCCY 1 cut(s) 1690
PscI ACATGT 1 cut(s) 1861
PshBI ATTAAT 1 cut(s) 746
Psp1406I AACGTT 1 cut(s) 636
Psp5II RGGWCCY 1 cut(s) 1690
Psp6I CCWGG 2 cut(s) 1258, 2156
PspEI GGTNACC 2 cut(s) 151, 2096
PspFI CCCAGC 1 cut(s) 1760
PspGI CCWGG 2 cut(s) 1258, 2156
PspN4I GGNNCC 7 cut(s) 82, 220, 877, 1691, 1692, 2063, 2262
PspPI GGNCC 4 cut(s) 201, 1391, 1690, 2260
PspPPI RGGWCCY 1 cut(s) 1690
PstNI CAGNNNCTG 1 cut(s) 1961
PsuI RGATCY 2 cut(s) 962, 1957
PvuII CAGCTG 2 cut(s) 1625, 1667
RsaI GTAC 2 cut(s) 724, 1174
RsaNI GTAC 2 cut(s) 723, 1173
RseI CAYNNNNRTG 4 cut(s) 834, 1152, 1443, 2106
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 4 cut(s) 633, 746, 1335, 1374
SatI GCNGC 8 cut(s) 13, 354, 672, 840, 923, 1129, 1565, 1665
Sau3AI GATC 5 cut(s) 758, 962, 1083, 1144, 1957
Sau96I GGNCC 4 cut(s) 201, 1391, 1690, 2260
SchI GAGTC 4 cut(s) 478, 575, 576, 2176
ScrFI CCNGG 6 cut(s) 174, 321, 322, 1260, 2158, 2163
SduI GDGCHC 3 cut(s) 334, 475, 1325
SfaNI GCATC 9 cut(s) 543, 625, 796, 802, 1197, 1394, 1435, 1719, 1725
SfcI CTRYAG 2 cut(s) 576, 1198
SgrDI CGTCGACG 1 cut(s) 212
SinI GGWCC 2 cut(s) 201, 1690
SmaI CCCGGG 1 cut(s) 322
SmiMI CAYNNNNRTG 4 cut(s) 834, 1152, 1443, 2106
SmlI CTYRAG 1 cut(s) 1791
SmoI CTYRAG 1 cut(s) 1791
SphI GCATGC 1 cut(s) 839
SseBI AGGCCT 1 cut(s) 2284
SspI AATATT 1 cut(s) 2050
SspMI CTAG 6 cut(s) 926, 954, 1449, 1494, 1919, 2366
StuI AGGCCT 1 cut(s) 2284
StyD4I CCNGG 6 cut(s) 172, 319, 320, 1258, 2156, 2161
StyI CCWWGG 3 cut(s) 129, 1056, 2285
TaaI ACNGT 6 cut(s) 1217, 1327, 1423, 2066, 2257, 2340
TaiI ACGT 3 cut(s) 27, 639, 724
TaqI TCGA 3 cut(s) 213, 487, 584
TaqII GACCGA 1 cut(s) 464
TauI GCSGC 3 cut(s) 15, 674, 842
TfiI GAWTC 9 cut(s) 344, 430, 452, 711, 986, 1543, 1841, 1889, 2357
Tru1I TTAA 4 cut(s) 633, 746, 1335, 1374
Tru9I TTAA 4 cut(s) 633, 746, 1335, 1374
TscAI CASTG 4 cut(s) 1156, 1330, 1540, 2367
TseFI GTSAC 5 cut(s) 137, 151, 1282, 1786, 2096
TseI GCWGC 5 cut(s) 353, 922, 1128, 1564, 1664
Tsp45I GTSAC 5 cut(s) 137, 151, 1282, 1786, 2096
TspGWI ACGGA 4 cut(s) 148, 199, 291, 562
TspMI CCCGGG 1 cut(s) 320
TspRI CASTG 4 cut(s) 1156, 1330, 1540, 2367
Van91I CCANNNNNTGG 1 cut(s) 2257
VpaK11BI GGWCC 2 cut(s) 201, 1690
VspI ATTAAT 1 cut(s) 746
XapI RAATTY 6 cut(s) 524, 693, 1032, 1376, 1405, 1452
XceI RCATGY 3 cut(s) 839, 1828, 1865
XcmI CCANNNNNNNNNTGG 2 cut(s) 289, 1974
XmaI CCCGGG 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 2 cut(s) 906, 1456
XspI CTAG 6 cut(s) 926, 954, 1449, 1494, 1919, 2366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.