Rw4G008770

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009414
Physical Location & Seq
Forward (+)
19405209 .. 19413509
8301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008770.1

Sequence Viewer

Length: 2415 bp
ATGATTGGGGGCGGCAAGAGCTACGTCTCAGCCCCACCGGCCTTCTCCAACGACGCCAAGCGCCTCCTCGTCTGCACCGGCGCCGCCGTCTCCGTCTTCAGCACCTCCACCGGCTTACAGATACTGTCGTTGGAGGGCCACACGGCGTTGGTCACCGCCGTCATAGTGGTTCCCGGTAACAAAGCCCTCAGCTTCTGCTGGACGGCCTCGCTTGACGGCACCGTTAGGTATTGGGACTTTGCCGCCGCCGAGTTGATGAAGACCATTGATATCAAGATGCCTATTTACTCCATGGTTATTCCGGCATTGTTGTTGGGCCAAACGGATTCGAAGGTGATGCCGGCCGATGCTTTTGCTTATTTGTCTGTTGAAAACACCACCCAAGGCAATGCACCCAAGGCTTTGCGTGGGCAGATTCGCAAGTGTAATTTGACTGAGTCTCGATTGGCTGGTGTTATTCTCGCAGAGAAAACCAGAAGTTATATCTATCAGTCCCTCAGGAAAATTCTTTGGCATCCGGACAAGCGGAAGCTTCATATCTGGAGAGCCCCTGCTAAAGATTCTAAACTCCCAGTGGCCAAGATTACACTACACCATACAAGAAATTTTACACTTCTTGCATTTCATCCAACAAAGAGGCTAGTTGCTGCAGGTGATACATCTGGGAGAATTCTAATCTGGAGAGGGTTTGGTAACCGTAAATTTTCGGATGGTGACAAACTTGTAAATCGGGGATCAATGAATGATGAGGAAGGAAGACCTGGGATCAGGGGAGATGATGATGCTGACTCTTGCACCACTTGGCATTGGCATCCTACTGGAATTAATCTCCTGCGTTTTTCTTCTGATGGAGCGTATTTATATTCAGGTGGAAAGGAAGGAGTTCTTGTGGACTGGCAGCTAGACACAGGGAAAAAGAAATTTTTACCAAGAATTGGATCTCCACTTCTATATTTAACTGATTCTCTAGATCCTTCACTTTCTGCTCTATCTTGTGCAGATAATCAAATTCACTTGCTAAAAATGCCCTCAATGGAAATCACAAAGTCTATTTCAGGGATCAAGCTCCCGTTTTCTTCTCTGGATGTATATGAAGGCATGTGCAGCAAGTTTGCCTTCGATGACACTGATGGGTTAGTTGCCTTGCGTACAGAGAACTATCGCATCCAGTTTTACAGCTTGTTTGATGACCGTGAAATTTCTGAGGTTCAAGTCTGTGAGAGAAACCATCAACCGGGCGATGAAGTCTCTGTAATAGTGACTTTGGTGTCTCTGTCTGGAGATGGCTCTATGATGAGCACAGTGGAAGTTACTCTTCCTGAGGATGGAATAGGTGGTCTTGTGTGTCTTAAGTTCTGGGCATCTGATTCGCAGAACAAAAATTTCAACTTGTCCACCATTGTGTATCAACCCCACAGGGATGCTGGAGTTTCTGCTCTTGCTTTTCATCCTACCCGTCAAATGGTTGTCAGTTCATCATATGGTGGGGATTTCAAGATTTGGGTTTGCAGTGACGAGATTCAGCAGAAACAAGATACACTACAGCATTCTGGGTGGATGTGCCATGCTGTAGGTTCATACAAGAAAAAGCCAATGACAGCTGCTGCATTTTCTGCTGATGGTTCTGTTCTGGCTGTTGCAGCTGAAACTGTAATCACACTATGGGACCCTGTGAACAACATGCTTGTGGCTGTGCTTGGACAGATGCAGATGCCAATTGTGACCCTCTCCTTTGCTGGGAATTCGGACTATCTTTTGTCTATCTCCCGGGGTTCAAAACCACAGTTATCTGTGTGGAGCATGTCAAAGCTGTCTGAGTCTTGGTCATACAAGCTTGACATAGAAGATGTTACGTGTGCGAAAGATTCATCATCATTTGCAGTTCTTGCCCTTTTGCCAAAATCATCTACTTTTATGGGCTCTAATGAACCAATTTTTCAAGGAAAAGATGGGGTAATCTTATTATTCAATGCAACAGATCCTGTTCCATTGGCTATGTGGTCCGTAAGGAAGGCCAAGGGAGGGGGACTTGCTTTCCTTAAAGGGAACTCCTTTCAACAACAAAGTATAGCGGATGGAAAATCACCGCAAGCTTTGCTTGCCTATATAAATGCCGATCATGAGTACATCCTCTTTGATCCATATGACTACGATGCACGTGAACTTAGTATGACCAGGGGGAAGGATCGTGTTGCCGTTGACGAAACAGCGCAATATGGATATGCATCTATCTATGGGGAGCTACCGCAATTTGAGATGAAGAGAGACCAGACCTTGTTAGCCCCATATGCTCCATCACAAAGGCCTTGGGAGACCATTTTTAGTGGGTCATCACATAATCTTCTACCCCTACCTAAATTGTGTTTAGAGTTTATGGAATCGCTGCTAGAAAGGAGGACTACCACAGTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
WD40

Protein Analysis

804

Amino Acids

88.51

Weight (kDa)

6.49

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR75_1st PF23869 15 - 100 7.2e-17 WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st PF24817 16 - 100 9.9e-06 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 266 - 335 1.8e-12 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR75_2nd PF23769 384 - 637 2.2e-35 WD repeat-containing protein 75 second beta-propeller
WD40_Prp19 PF24814 451 - 613 1.1e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 473 - 608 4.3e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 476 - 610 1.8e-08 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 641
AasI GACNNNNNNGTC 1 cut(s) 1267
Acc36I ACCTGC 1 cut(s) 641
AccB1I GGYRCC 2 cut(s) 80, 218
AccB7I CCANNNNNTGG 1 cut(s) 935
AccIII TCCGGA 1 cut(s) 517
AciI CCGC 9 cut(s) 12, 84, 156, 243, 246, 526, 2072, 2087, 2246
AclWI GGATC 8 cut(s) 742, 773, 946, 965, 1067, 1973, 2132, 2193
AcoI YGGCCR 2 cut(s) 342, 576
AcsI RAATTY 9 cut(s) 504, 604, 669, 701, 920, 1008, 1197, 1381, 1741
AcuI CTGAAG 1 cut(s) 82
AcvI CACGTG 1 cut(s) 2159
AcyI GRCGYC 2 cut(s) 54, 81
AfaI GTAC 2 cut(s) 1150, 2126
AfiI CCNNNNNNNGG 6 cut(s) 935, 1234, 1325, 1462, 1737, 2022
AflII CTTAAG 1 cut(s) 1349
AflIII ACRYGT 1 cut(s) 1853
AgsI TTSAA 8 cut(s) 371, 1211, 1387, 1495, 1776, 1940, 1969, 2057
AjnI CCWGG 2 cut(s) 760, 2174
AleI CACNNNNGTG 1 cut(s) 1400
Alw21I GWGCWC 1 cut(s) 1301
Alw26I GTCTC 7 cut(s) 31, 94, 444, 1252, 1275, 2259, 2306
AlwI GGATC 8 cut(s) 742, 773, 946, 965, 1067, 1973, 2132, 2193
AlwNI CAGNNNCTG 4 cut(s) 124, 195, 1604, 1982
Ama87I CYCGRG 1 cut(s) 1767
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 8 cut(s) 39, 136, 204, 316, 342, 576, 2013, 2303
ApeKI GCWGC 7 cut(s) 647, 898, 1104, 1601, 1604, 1640, 2383
ApoI RAATTY 9 cut(s) 504, 604, 669, 701, 920, 1008, 1197, 1381, 1741
AseI ATTAAT 1 cut(s) 825
Asp700I GAANNNNTTC 1 cut(s) 882
AspLEI GCGC 3 cut(s) 63, 83, 2212
AspS9I GGNCC 4 cut(s) 136, 316, 1666, 2001
AsuC2I CCSGG 4 cut(s) 174, 1236, 1768, 1769
AsuHPI GGTGA 5 cut(s) 145, 346, 665, 725, 2076
AsuII TTCGAA 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 1767
AvaII GGWCC 2 cut(s) 1666, 2001
AxyI CCTNAGG 2 cut(s) 497, 1320
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BalI TGGCCA 1 cut(s) 578
BanI GGYRCC 2 cut(s) 80, 218
BanII GRGCYC 2 cut(s) 550, 1922
BbrPI CACGTG 1 cut(s) 2159
BbsI GAAGAC 3 cut(s) 88, 266, 763
Bbv12I GWGCWC 1 cut(s) 1301
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 7 cut(s) 634, 910, 1116, 1588, 1591, 1652, 2370
BceAI ACGGC 6 cut(s) 71, 143, 159, 219, 232, 2180
BcgI CGANNNNNNTGC 6 cut(s) 319, 335, 353, 369, 1350, 1384
BciT130I CCWGG 2 cut(s) 762, 2176
BcnI CCSGG 4 cut(s) 174, 1236, 1768, 1769
BcoDI GTCTC 7 cut(s) 31, 94, 444, 1252, 1275, 2259, 2306
BfaI CTAG 4 cut(s) 641, 902, 968, 2387
BfmI CTRYAG 3 cut(s) 648, 1541, 1569
BfoI RGCGCY 2 cut(s) 64, 84
BfrI CTTAAG 1 cut(s) 1349
BfuAI ACCTGC 1 cut(s) 641
BglI GCCNNNNNGGC 1 cut(s) 38
Bme1390I CCNGG 6 cut(s) 174, 762, 1236, 1768, 1769, 2176
Bme18I GGWCC 2 cut(s) 1666, 2001
BmeT110I CYCGRG 1 cut(s) 1767
BmgT120I GGNCC 4 cut(s) 136, 316, 1666, 2001
BmiI GGNNCC 5 cut(s) 82, 171, 220, 1667, 1668
BmrFI CCNGG 6 cut(s) 174, 762, 1236, 1768, 1769, 2176
BmrI ACTGGG 1 cut(s) 566
BmuI ACTGGG 1 cut(s) 566
BpiI GAAGAC 3 cut(s) 88, 266, 763
BpmI CTGGAG 4 cut(s) 562, 700, 1299, 1446
Bpu10I CCTNAGC 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 329
BpuMI CCSGG 4 cut(s) 174, 1236, 1768, 1769
BsaAI YACGTR 2 cut(s) 1854, 2159
BsaBI GATNNNNATC 1 cut(s) 2224
BsaHI GRCGYC 2 cut(s) 54, 81
BsaI GGTCTC 2 cut(s) 2259, 2306
BsaJI CCNNGG 9 cut(s) 291, 382, 396, 761, 1767, 1768, 2016, 2175, 2306
BsaWI WCCGGW 1 cut(s) 517
BsaXI ACNNNNNCTCC 2 cut(s) 873, 903
Bsc4I CCNNNNNNNGG 6 cut(s) 935, 1234, 1325, 1462, 1737, 2022
Bse118I RCCGGY 4 cut(s) 37, 77, 110, 340
Bse1I ACTGG 4 cut(s) 572, 823, 899, 1168
Bse21I CCTNAGG 2 cut(s) 497, 1320
Bse3DI GCAATG 1 cut(s) 394
Bse8I GATNNNNATC 1 cut(s) 2224
BseAI TCCGGA 1 cut(s) 517
BseBI CCWGG 2 cut(s) 762, 2176
BseDI CCNNGG 9 cut(s) 291, 382, 396, 761, 1767, 1768, 2016, 2175, 2306
BseJI GATNNNNATC 1 cut(s) 2224
BseLI CCNNNNNNNGG 6 cut(s) 935, 1234, 1325, 1462, 1737, 2022
BseMI GCAATG 1 cut(s) 394
BseMII CTCAG 7 cut(s) 42, 202, 426, 511, 1194, 1311, 1806
BseNI ACTGG 4 cut(s) 572, 823, 899, 1168
BseRI GAGGAG 1 cut(s) 56
BseX3I CGGCCG 1 cut(s) 342
BseXI GCAGC 7 cut(s) 634, 910, 1116, 1588, 1591, 1652, 2370
BseYI CCCAGC 1 cut(s) 1736
BsgI GTGCAG 3 cut(s) 58, 1017, 1123
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 8 cut(s) 41, 138, 206, 318, 344, 578, 2015, 2305
BshNI GGYRCC 2 cut(s) 80, 218
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 1301
BsiHKCI CYCGRG 1 cut(s) 1767
BsiSI CCGG 9 cut(s) 38, 78, 111, 174, 302, 341, 518, 1235, 1768
BslFI GGGAC 4 cut(s) 248, 478, 1679, 2040
BslI CCNNNNNNNGG 6 cut(s) 935, 1234, 1325, 1462, 1737, 2022
BsmAI GTCTC 7 cut(s) 31, 94, 444, 1252, 1275, 2259, 2306
BsmBI CGTCTC 2 cut(s) 31, 94
BsmFI GGGAC 4 cut(s) 248, 478, 1679, 2040
BsmI GAATGC 1 cut(s) 1546
BsnI GGCC 8 cut(s) 41, 138, 206, 318, 344, 578, 2015, 2305
Bso31I GGTCTC 2 cut(s) 2259, 2306
BsoBI CYCGRG 1 cut(s) 1767
Bsp119I TTCGAA 1 cut(s) 329
Bsp1286I GDGCHC 3 cut(s) 550, 1301, 1922
Bsp13I TCCGGA 1 cut(s) 517
Bsp143I GATC 9 cut(s) 734, 765, 938, 970, 1059, 1978, 2116, 2137, 2185
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 9 cut(s) 12, 84, 156, 243, 246, 526, 2072, 2087, 2246
BspANI GGCC 8 cut(s) 41, 138, 206, 318, 344, 578, 2015, 2305
BspCNI CTCAG 7 cut(s) 41, 201, 427, 510, 1195, 1312, 1807
BspEI TCCGGA 1 cut(s) 517
BspHI TCATGA 1 cut(s) 2119
BspLI GGNNCC 5 cut(s) 82, 171, 220, 1667, 1668
BspMAI CTGCAG 1 cut(s) 652
BspMI ACCTGC 1 cut(s) 641
BspPI GGATC 8 cut(s) 742, 773, 946, 965, 1067, 1973, 2132, 2193
BspT104I TTCGAA 1 cut(s) 329
BspT107I GGYRCC 2 cut(s) 80, 218
BspTI CTTAAG 1 cut(s) 1349
BspTNI GGTCTC 2 cut(s) 2259, 2306
BsrDI GCAATG 1 cut(s) 394
BsrFI RCCGGY 4 cut(s) 37, 77, 110, 340
BsrI ACTGG 4 cut(s) 572, 823, 899, 1168
BssAI RCCGGY 4 cut(s) 37, 77, 110, 340
BssECI CCNNGG 9 cut(s) 291, 382, 396, 761, 1767, 1768, 2016, 2175, 2306
BssMI GATC 9 cut(s) 734, 765, 938, 970, 1059, 1978, 2116, 2137, 2185
BssNI GRCGYC 2 cut(s) 54, 81
BssT1I CCWWGG 5 cut(s) 291, 382, 396, 2016, 2306
Bst2UI CCWGG 2 cut(s) 762, 2176
Bst4CI ACNGT 8 cut(s) 126, 223, 698, 1193, 1303, 1651, 1785, 2407
Bst6I CTCTTC 2 cut(s) 1320, 2255
BstACI GRCGYC 2 cut(s) 54, 81
BstAFI CTTAAG 1 cut(s) 1349
BstAPI GCANNNNNTGC 3 cut(s) 1613, 1886, 2095
BstBAI YACGTR 2 cut(s) 1854, 2159
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 3 cut(s) 342, 2091, 2100
BstDEI CTNAG 8 cut(s) 28, 188, 435, 497, 1203, 1320, 1815, 2165
BstDSI CCRYGG 1 cut(s) 291
BstEII GGTNACC 2 cut(s) 151, 692
BstH2I RGCGCY 2 cut(s) 64, 84
BstHHI GCGC 3 cut(s) 63, 83, 2212
BstKTI GATC 9 cut(s) 737, 768, 941, 973, 1062, 1981, 2119, 2140, 2188
BstMAI GTCTC 7 cut(s) 31, 94, 444, 1252, 1275, 2259, 2306
BstMBI GATC 9 cut(s) 734, 765, 938, 970, 1059, 1978, 2116, 2137, 2185
BstMCI CGRYCG 1 cut(s) 345
BstNI CCWGG 2 cut(s) 762, 2176
BstNSI RCATGY 3 cut(s) 1102, 1684, 1804
BstPI GGTNACC 2 cut(s) 151, 692
BstSCI CCNGG 6 cut(s) 172, 760, 1234, 1766, 1767, 2174
BstSFI CTRYAG 3 cut(s) 648, 1541, 1569
BstV1I GCAGC 7 cut(s) 634, 910, 1116, 1588, 1591, 1652, 2370
BstV2I GAAGAC 3 cut(s) 88, 266, 763
BstX2I RGATCY 3 cut(s) 938, 970, 1978
BstYI RGATCY 3 cut(s) 938, 970, 1978
BstZI CGGCCG 1 cut(s) 342
Bsu36I CCTNAGG 2 cut(s) 497, 1320
BsuRI GGCC 8 cut(s) 41, 138, 206, 318, 344, 578, 2015, 2305
BtgI CCRYGG 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 1254
BtsI GCAGTG 1 cut(s) 1516
BtsIMutI CAGTG 4 cut(s) 579, 1125, 1308, 1516
BveI ACCTGC 1 cut(s) 641
Cac8I GCNNGC 3 cut(s) 342, 2091, 2100
CaiI CAGNNNCTG 4 cut(s) 124, 195, 1604, 1982
CciI TCATGA 1 cut(s) 2119
CfoI GCGC 3 cut(s) 63, 83, 2212
Cfr10I RCCGGY 4 cut(s) 37, 77, 110, 340
Cfr13I GGNCC 4 cut(s) 136, 316, 1666, 2001
Cfr9I CCCGGG 1 cut(s) 1767
CseI GACGC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 1149, 2125
CviAII CATG 6 cut(s) 292, 1099, 1565, 1681, 1801, 2120
CviQI GTAC 2 cut(s) 1149, 2125
DdeI CTNAG 8 cut(s) 28, 188, 435, 497, 1203, 1320, 1815, 2165
DinI GGCGCC 1 cut(s) 82
DpnI GATC 9 cut(s) 736, 767, 940, 972, 1061, 1980, 2118, 2139, 2187
DpnII GATC 9 cut(s) 734, 765, 938, 970, 1059, 1978, 2116, 2137, 2185
DrdI GACNNNNNNGTC 1 cut(s) 1267
DseDI GACNNNNNNGTC 1 cut(s) 1267
EaeI YGGCCR 2 cut(s) 342, 576
EagI CGGCCG 1 cut(s) 342
Eam1104I CTCTTC 2 cut(s) 1320, 2255
EarI CTCTTC 2 cut(s) 1320, 2255
EclXI CGGCCG 1 cut(s) 342
Eco130I CCWWGG 5 cut(s) 291, 382, 396, 2016, 2306
Eco147I AGGCCT 1 cut(s) 2305
Eco24I GRGCYC 2 cut(s) 550, 1922
Eco31I GGTCTC 2 cut(s) 2259, 2306
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 1666, 2001
Eco52I CGGCCG 1 cut(s) 342
Eco57I CTGAAG 1 cut(s) 82
Eco72I CACGTG 1 cut(s) 2159
Eco81I CCTNAGG 2 cut(s) 497, 1320
Eco88I CYCGRG 1 cut(s) 1767
Eco91I GGTNACC 2 cut(s) 151, 692
EcoO109I RGGNCCY 1 cut(s) 1666
EcoO65I GGTNACC 2 cut(s) 151, 692
EcoRI GAATTC 2 cut(s) 669, 1741
EcoRII CCWGG 2 cut(s) 760, 2174
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 5 cut(s) 291, 382, 396, 2016, 2306
EcoT22I ATGCAT 1 cut(s) 2227
EcoT38I GRGCYC 2 cut(s) 550, 1922
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
ErhI CCWWGG 5 cut(s) 291, 382, 396, 2016, 2306
Esp3I CGTCTC 2 cut(s) 31, 94
FaeI CATG 6 cut(s) 295, 1102, 1568, 1684, 1804, 2123
FalI AAGNNNNNCTT 8 cut(s) 870, 902, 1100, 1132, 1299, 1331, 2082, 2114
FaqI GGGAC 4 cut(s) 248, 478, 1679, 2040
FatI CATG 6 cut(s) 291, 1098, 1564, 1680, 1800, 2119
FauNDI CATATG 3 cut(s) 1480, 2143, 2287
FriOI GRGCYC 2 cut(s) 550, 1922
FspBI CTAG 4 cut(s) 641, 902, 968, 2387
GlaI GCGC 3 cut(s) 62, 82, 2211
GsaI CCCAGC 1 cut(s) 1740
GsuI CTGGAG 4 cut(s) 562, 700, 1299, 1446
HaeII RGCGCY 2 cut(s) 64, 84
HaeIII GGCC 8 cut(s) 41, 138, 206, 318, 344, 578, 2015, 2305
HapII CCGG 9 cut(s) 38, 78, 111, 174, 302, 341, 518, 1235, 1768
HgaI GACGC 1 cut(s) 62
HhaI GCGC 3 cut(s) 63, 83, 2212
Hin1I GRCGYC 2 cut(s) 54, 81
Hin1II CATG 6 cut(s) 295, 1102, 1568, 1684, 1804, 2123
Hin6I GCGC 3 cut(s) 61, 81, 2210
HinP1I GCGC 3 cut(s) 61, 81, 2210
HincII GTYRAC 1 cut(s) 2200
HindII GTYRAC 1 cut(s) 2200
HindIII AAGCTT 3 cut(s) 530, 1832, 2091
HpaII CCGG 9 cut(s) 38, 78, 111, 174, 302, 341, 518, 1235, 1768
HphI GGTGA 5 cut(s) 145, 346, 665, 725, 2076
Hpy166II GTNNAC 5 cut(s) 892, 1395, 1675, 2162, 2200
Hpy188I TCNGA 6 cut(s) 709, 847, 1204, 1366, 1747, 1816
Hpy8I GTNNAC 5 cut(s) 892, 1395, 1675, 2162, 2200
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 9 cut(s) 52, 325, 746, 872, 984, 1088, 1126, 2005, 2176
HpyCH4III ACNGT 8 cut(s) 126, 223, 698, 1193, 1303, 1651, 1785, 2407
HpyCH4IV ACGT 3 cut(s) 24, 1853, 2158
HpyF3I CTNAG 8 cut(s) 28, 188, 435, 497, 1203, 1320, 1815, 2165
HpySE526I ACGT 3 cut(s) 24, 1853, 2158
Hsp92I GRCGYC 2 cut(s) 54, 81
Hsp92II CATG 6 cut(s) 295, 1102, 1568, 1684, 1804, 2123
HspAI GCGC 3 cut(s) 61, 81, 2210
KasI GGCGCC 1 cut(s) 80
KflI GGGWCCC 1 cut(s) 1666
Kpn2I TCCGGA 1 cut(s) 517
KroI GCCGGC 1 cut(s) 340
KroNI GCCGGC 1 cut(s) 342
Kzo9I GATC 9 cut(s) 734, 765, 938, 970, 1059, 1978, 2116, 2137, 2185
LmnI GCTCC 5 cut(s) 851, 1071, 1797, 2239, 2296
Lsp1109I GCAGC 7 cut(s) 634, 910, 1116, 1588, 1591, 1652, 2370
MaeI CTAG 4 cut(s) 641, 902, 968, 2387
MaeII ACGT 3 cut(s) 24, 1853, 2158
MaeIII GTNAC 9 cut(s) 151, 176, 692, 713, 1258, 1309, 1511, 1720, 1849
MalI GATC 9 cut(s) 736, 767, 940, 972, 1061, 1980, 2118, 2139, 2187
MboI GATC 9 cut(s) 734, 765, 938, 970, 1059, 1978, 2116, 2137, 2185
MboII GAAGA 9 cut(s) 88, 271, 768, 834, 1068, 1307, 1856, 2272, 2333
MfeI CAATTG 1 cut(s) 1716
MflI RGATCY 3 cut(s) 938, 970, 1978
MhlI GDGCHC 3 cut(s) 550, 1301, 1922
MlsI TGGCCA 1 cut(s) 578
MluNI TGGCCA 1 cut(s) 578
Mly113I GGCGCC 1 cut(s) 81
MlyI GAGTC 3 cut(s) 446, 782, 1826
MmeI TCCRAC 3 cut(s) 72, 111, 653
Mox20I TGGCCA 1 cut(s) 578
Mph1103I ATGCAT 1 cut(s) 2227
MroI TCCGGA 1 cut(s) 517
MroNI GCCGGC 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 882
MscI TGGCCA 1 cut(s) 578
MseI TTAA 4 cut(s) 825, 956, 1350, 2040
MslI CAYNNNNRTG 3 cut(s) 1400, 1419, 1685
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 2 cut(s) 1601, 1643
MspCI CTTAAG 1 cut(s) 1349
MspI CCGG 9 cut(s) 38, 78, 111, 174, 302, 341, 518, 1235, 1768
MspR9I CCNGG 6 cut(s) 174, 762, 1236, 1768, 1769, 2176
MunI CAATTG 1 cut(s) 1716
Mva1269I GAATGC 1 cut(s) 1546
MvaI CCWGG 2 cut(s) 762, 2176
NaeI GCCGGC 1 cut(s) 342
NarI GGCGCC 1 cut(s) 81
NciI CCSGG 4 cut(s) 174, 1236, 1768, 1769
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 3 cut(s) 1480, 2143, 2287
NdeII GATC 9 cut(s) 734, 765, 938, 970, 1059, 1978, 2116, 2137, 2185
NgoMIV GCCGGC 1 cut(s) 340
NlaIII CATG 6 cut(s) 295, 1102, 1568, 1684, 1804, 2123
NlaIV GGNNCC 5 cut(s) 82, 171, 220, 1667, 1668
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 5 cut(s) 151, 713, 1258, 1511, 1720
NsiI ATGCAT 1 cut(s) 2227
NspI RCATGY 3 cut(s) 1102, 1684, 1804
NspV TTCGAA 1 cut(s) 329
OliI CACNNNNGTG 1 cut(s) 1400
PagI TCATGA 1 cut(s) 2119
PaqCI CACCTGC 1 cut(s) 641
PceI AGGCCT 1 cut(s) 2305
PctI GAATGC 1 cut(s) 1546
PdiI GCCGGC 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 8 cut(s) 326, 415, 560, 962, 1367, 1519, 1865, 2378
PflFI GACNNNGTC 1 cut(s) 436
PflMI CCANNNNNTGG 1 cut(s) 935
PleI GAGTC 3 cut(s) 445, 782, 1825
PluTI GGCGCC 1 cut(s) 84
PmaCI CACGTG 1 cut(s) 2159
PmlI CACGTG 1 cut(s) 2159
PpsI GAGTC 3 cut(s) 445, 782, 1825
Ppu21I YACGTR 2 cut(s) 1854, 2159
PpuMI RGGWCCY 1 cut(s) 1666
PshBI ATTAAT 1 cut(s) 825
Psp5II RGGWCCY 1 cut(s) 1666
Psp6I CCWGG 2 cut(s) 760, 2174
PspCI CACGTG 1 cut(s) 2159
PspEI GGTNACC 2 cut(s) 151, 692
PspFI CCCAGC 1 cut(s) 1736
PspGI CCWGG 2 cut(s) 760, 2174
PspN4I GGNNCC 5 cut(s) 82, 171, 220, 1667, 1668
PspPI GGNCC 4 cut(s) 136, 316, 1666, 2001
PspPPI RGGWCCY 1 cut(s) 1666
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 4 cut(s) 124, 195, 1604, 1982
PsuI RGATCY 3 cut(s) 938, 970, 1978
PsyI GACNNNGTC 1 cut(s) 436
PvuII CAGCTG 2 cut(s) 1601, 1643
RsaI GTAC 2 cut(s) 1150, 2126
RsaNI GTAC 2 cut(s) 1149, 2125
RseI CAYNNNNRTG 3 cut(s) 1400, 1419, 1685
SaqAI TTAA 4 cut(s) 825, 956, 1350, 2040
Sau3AI GATC 9 cut(s) 734, 765, 938, 970, 1059, 1978, 2116, 2137, 2185
Sau96I GGNCC 4 cut(s) 136, 316, 1666, 2001
SchI GAGTC 3 cut(s) 446, 782, 1826
ScrFI CCNGG 6 cut(s) 174, 762, 1236, 1768, 1769, 2176
SduI GDGCHC 3 cut(s) 550, 1301, 1922
SfcI CTRYAG 3 cut(s) 648, 1541, 1569
SfoI GGCGCC 1 cut(s) 82
SfuI TTCGAA 1 cut(s) 329
SgrAI CRCCGGYG 1 cut(s) 77
SinI GGWCC 2 cut(s) 1666, 2001
SmaI CCCGGG 1 cut(s) 1769
SmiMI CAYNNNNRTG 3 cut(s) 1400, 1419, 1685
SmlI CTYRAG 1 cut(s) 1349
SmoI CTYRAG 1 cut(s) 1349
SseBI AGGCCT 1 cut(s) 2305
SsiI CCGC 9 cut(s) 12, 84, 156, 243, 246, 526, 2072, 2087, 2246
SspDI GGCGCC 1 cut(s) 80
SspMI CTAG 4 cut(s) 641, 902, 968, 2387
StuI AGGCCT 1 cut(s) 2305
StyD4I CCNGG 6 cut(s) 172, 760, 1234, 1766, 1767, 2174
StyI CCWWGG 5 cut(s) 291, 382, 396, 2016, 2306
TaaI ACNGT 8 cut(s) 126, 223, 698, 1193, 1303, 1651, 1785, 2407
TaiI ACGT 3 cut(s) 27, 1856, 2161
TaqI TCGA 3 cut(s) 329, 442, 1119
TatI WGTACW 1 cut(s) 2124
TauI GCSGC 4 cut(s) 15, 86, 245, 248
TfiI GAWTC 8 cut(s) 326, 415, 560, 962, 1367, 1519, 1865, 2378
Tru1I TTAA 4 cut(s) 825, 956, 1350, 2040
Tru9I TTAA 4 cut(s) 825, 956, 1350, 2040
TscAI CASTG 4 cut(s) 579, 1132, 1308, 1516
TseFI GTSAC 5 cut(s) 151, 713, 1258, 1511, 1720
TseI GCWGC 7 cut(s) 647, 898, 1104, 1601, 1604, 1640, 2383
Tsp45I GTSAC 5 cut(s) 151, 713, 1258, 1511, 1720
TspGWI ACGGA 3 cut(s) 82, 338, 1993
TspMI CCCGGG 1 cut(s) 1767
TspRI CASTG 4 cut(s) 579, 1132, 1308, 1516
Tth111I GACNNNGTC 1 cut(s) 436
Van91I CCANNNNNTGG 1 cut(s) 935
Vha464I CTTAAG 1 cut(s) 1349
VpaK11BI GGWCC 2 cut(s) 1666, 2001
VspI ATTAAT 1 cut(s) 825
XapI RAATTY 9 cut(s) 504, 604, 669, 701, 920, 1008, 1197, 1381, 1741
XbaI TCTAGA 1 cut(s) 967
XceI RCATGY 3 cut(s) 1102, 1684, 1804
XcmI CCANNNNNNNNNTGG 2 cut(s) 1421, 1995
XmaI CCCGGG 1 cut(s) 1767
XmnI GAANNNNTTC 1 cut(s) 882
XspI CTAG 4 cut(s) 641, 902, 968, 2387
Zsp2I ATGCAT 1 cut(s) 2227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.