Rroxscaffold_5G00346170

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009414
Physical Location & Seq
Forward (+)
16901737 .. 16916101
14365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00346170.1

Sequence Viewer

Length: 3720 bp
ATGATTGGGGGCGGCAAGAGCTACGTCTCAGCCCCACCGGCCTTCTCCAACGACGCCAAGCGCCTCCTCGTCTGCACCAGCACCGTCGTCTCCGTCTTCAGCACCTCCACCGGCTTACAGATACTGTCGTTGGAGGGCCACACGGCGTTGGTCACCGCCGTCATAGTGGTTCCCGGTAACAAAGCCCTCAGCTTCTGCTGGACGGCCTCGCTTGACGGCACCGTTAGGTATTGGGACTTTGCCGCCGCCGAGTTGATGAAGACCATTGATATCAAAATGCCTATTTACTCCATGGTTATTCCGGCGTTGTTGTTGGGCCAAACGGATTCGAAGGTGATGCCGGCCGATGCTTTTGCTTATTTGTCTGTTGAAAACACCACCCAAGTGCAAGACAATGCACCCAAGCCTTTGCGTGGGCAGATTCGCAAGTGTAATTTGACTGAATCTCGATTGGCTGGTGTTATTCTCGCAGAGACTAGAAAACCAGAAGTTATATCTATCAGTCCCTCTGGAAAATTCTTTGGCATCCGGAACAAGCGGAAGCTTCATATCTGGAGAGCCCCTGCTAAAGATTCTAAACTCCCAGTGGCCAAGATTACGCTACACCATACAAGAAATTTTACAGTTCTTGCATTTCATCCAACAAAGAGGCTAGTTGCTGCAGGTGATACATCTGGGAGAATTTTAATCTGGAGAGGGTTTGGTAACCGTAAATTTTCGGATGGTGACAAACTAGTAAATCGGGGATCAATGAATGATGAGGAAGGAAGACCTGGGATCAGGGGAGATGATGATGCTGACTCTTGCACCACTTGGCATTGGCATCCTATTGGAATTAATCTCCTGCGTTTTTCTTCTGATGGAGCGTATTTATATTCAGGTGGAAAGGAAGGAGTTCTTGTGGACTGGCAGCTAGACACAGGGAAAAAGAAATTTTTACCAAGAATTGGATCTCCACTTCTATATTTAACTGATTCTCTAGATCCTTCACTTTCTTCTGTAAATAATCAAATTCACTTGCTAAAAATGCCCTCAATGGAAATCACAAAGTCTATTTCAGGGATCAAGCTCCCTATTTCTTCTCCGGACGTATATGAAGGCATATGCAGTAAGTTTGCCTTCAATGACACTGATGGGTTAGTTGCCTTGCGTACAGAGAACTATCGCATCCAGTTTTACAGCTTGTTTGATGACCGTGAAATTTCTGAGGTACAAGTCTGTGACAGAAACCATCAACCAGGCGATGAAGTCTCTGTAATAGTGACTTTGGTGTCTCTGTCTGGAGATAGCTCTATGATGAGCACAGTGGACGTTAATCTTCCTGAGGATGGAATAGGTGGTCTTGTGTGTCTTAAGTTCTGGGCATCAGATTCGCAGAACAAAAATTTCAACTTGTCCACCATTGTGTATGAACCTCACAGGGATGCTGGAGTTTCTGCTCTTGCTTTTCATCCTACCCGTCAAATGGTTGTCAGTTCATCATATGGTGGGGATTTCAAGATTTGGGTTTGCAGTGACGAGATTCAGCAGAAACAAGATACACTACAGCATTCTGGGTGGATGTGCCATGCTGTAGGTTCATACAAGAAAAAGCCAATGACAGCTGCTGCATTTTCTGCCGATGGTTCTGTTCTGGCTGTTGCAGCTGAAACTGTAATCACACTATGGGACCCTGTGAACAACATGCTTGTGGCTGTGCTTGGAGAGATGCAGATGCCAATTGTGACCCTCTCCTTTGCTGGGAATTCGGACTATCTTTTGTCTATCTCCCGGGGTTCAAAACCACAGTTATCTGTGTGGAGCATGTCAAAGCTGTCTGAGTCTTGGTCATACAAGCTTGACATAGAAGATGTTACGTGTGCGAAAGATTCATCATCATTTGCAGTTCTTGCCCTTTTGCCAAAATCATCTACTTTTATGGGCTCTAATGAACCAATTTTTCAAGGAAAAGATGGGGTAATCTTATTATTCAATGCAACAGATCCTGTTCCATTGGCTATGTGGTCCGTAAGGAAGGCCAAGGGAGGGGGACTTGCTTTCCTTAAAGGGAACTCACCTTCCTTTCAACAACAAAGTATAGCGGATGGAAAATCACCACAAGCTTTGCTTGCCTATATAAATGCCGATCATGAGTACATCCTCTTTGATCCATATGACTACGATGCACGTGAACTTAGTATGACCAGGGGGAAGGATCGTGTTGCCGTTGACGAAACAGCGGAATATGGATATGCATCTATCTATGGGGAGCTACCGCAATTTGAGATGAAGAGAGACCAGACCTTGTTAGCCCCATATGCTCCATCACAAAGGCCTTGGGAGACCATTTTTATTCTTTTGTCAAGTTTGTGTGGGATTGCTACCTCACAAACCATAGTCACCAGTTTACCTGGTTTTGAAGGGGACCTGCCATTCACACTTGAAACTGGGTATGTGGGTGTGGGTGACTCAAACTCCTCCCAATTGTTCTACTATTTTGTTGAGTCATCGAGAAGCCCTTCCGAAGACCCTCTTATGCTTTGGTTCACTGGAGGCCCTGGCTGCTCTGTTCTCTCTGCCTTCTTTTATGAAAGTGGTCCACTAGCTTTCACATATGTAGATTACAATGGAAGTCTACCCTCACTTCATGTGAATCCATATGCATGGACACAGGGCCTCAACATAATATATGTAGATTCACCTGTTGGCACCGGATTCTCTTACTCTACAACCCAAGAAGGTTACTATATAGATGACGAAGATGCCGTGGACCAGAGTGTTGAGTTTCTGAGGAAGTGGTTGTTGGCTCATCCTCAATATTTGAACAATATACTCTACATTGGTGGCGATTCTTATTCAGGAATTCCACTTCCTATAATAGTGCAACAACTTTTTGATGGTAACAATGCTGGAGTGGAGCCCTATCTAAACCTTCGAGTACATAATTCTTTTCTCCAGAACTCAGATGGTTATATACTCGGAAACCCGAAGACTGACGACTTCATTGATGTCAACTCAGTGAACCCATTTGCACACAGGTTGACACTCATATCAGATGAACTTTATGAGTCTACCAAAGAAAGCTGCGGCGGTGATTACGTTAATGTAGACGATAACAACACAGTATGTGTGACAGATCTTGCAACTATTGATCAGCTGCTTCTTCAAATTAATATTATGCATGTCTTGGAACCCAATTGTCAAGTGGCCTCACCAAACAACAGAGAAGAAGAAGGAAGAAGATCAATCCGAGAACTACCTGAGAATTTTCTAACAGAATCTACTCCTGCATTGTGGTGCCGGTACTACAACTATGTGATGTTGGGTGTGTGGGCTAATGACCACAGTGTCCGCAAAGCTCTTAATGTCAGCGAGAACTCTATTGGTGTTTGGAAGAGGTGTAATATGAGCATAGCTTACACAAAGACCGTCACAAGTAGCGTTGAGTATCATAAGAATCTCAGCAACACAAACCTTCGAGCTCTTGTATACAGTGGTGACCATGACATGTCTGTTCCACACATTGGCACCCAGGAGTGGATCCGTGATCTAAATTTGACAAGTAGTGAGATTTGGCGGCCGTGGTATGTAGATGGTCAAGTTGCTGGATACTCAGAAAAGTTCTCAAACGTTGACTACAATCTGCAATATGCAACTGGTGCGGGTCATGTGGCTCCAGAGTACAAAGCTAAGGAATGTTATGCGATGATTGATCGTTGGCTCGCTTATTACCCCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
WD40

Protein Analysis

1239

Amino Acids

137.17

Weight (kDa)

5.21

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR75_1st PF23869 15 - 238 2e-23 WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st PF24817 15 - 228 2.4e-09 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 270 - 333 5.2e-11 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR75_2nd PF23769 386 - 638 4.1e-33 WD repeat-containing protein 75 second beta-propeller
WD40_Prp19 PF24814 452 - 614 2.1e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 474 - 608 1.1e-07 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 475 - 603 4.1e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 477 - 612 2.3e-08 WDR5 beta-propeller domain
Peptidase_S10 PF00450 797 - 1235 3.1e-109 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 653
AasI GACNNNNNNGTC 2 cut(s) 1270, 3326
Acc36I ACCTGC 2 cut(s) 653, 2415
AccB1I GGYRCC 4 cut(s) 218, 2687, 3276, 3506
AccB7I CCANNNNNTGG 2 cut(s) 947, 3503
AccI GTMKAC 4 cut(s) 2614, 3050, 3087, 3468
AccIII TCCGGA 2 cut(s) 528, 1084
AclI AACGTT 1 cut(s) 3609
AcoI YGGCCR 3 cut(s) 342, 588, 3557
AcuI CTGAAG 1 cut(s) 82
AcvI CACGTG 1 cut(s) 2168
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 6 cut(s) 1153, 1212, 2135, 2919, 3284, 3662
AfiI CCNNNNNNNGG 8 cut(s) 413, 947, 1328, 1465, 1740, 2025, 3503, 3516
AflII CTTAAG 1 cut(s) 1352
AflIII ACRYGT 2 cut(s) 1856, 3486
AhlI ACTAGT 1 cut(s) 733
AjnI CCWGG 6 cut(s) 772, 1237, 2183, 2389, 2536, 3510
AjuI GAANNNNNNNTTGG 2 cut(s) 2765, 2797
AleI CACNNNNGTG 2 cut(s) 383, 1403
AloI GAACNNNNNNTCC 2 cut(s) 2042, 2074
Alw21I GWGCWC 2 cut(s) 1304, 3463
Alw26I GTCTC 7 cut(s) 31, 94, 467, 1255, 1278, 2268, 2315
AlwNI CAGNNNCTG 4 cut(s) 124, 195, 1607, 1985
Ama87I CYCGRG 1 cut(s) 1770
Aor13HI TCCGGA 2 cut(s) 528, 1084
ApeKI GCWGC 8 cut(s) 659, 910, 1604, 1607, 1643, 2541, 3063, 3136
AseI ATTAAT 2 cut(s) 837, 3150
Asp700I GAANNNNTTC 2 cut(s) 894, 2497
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 9 cut(s) 136, 316, 1669, 2004, 2404, 2534, 2576, 2653, 2749
AsuC2I CCSGG 3 cut(s) 174, 1771, 1772
AsuII TTCGAA 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 1770
AvaII GGWCC 5 cut(s) 1669, 2004, 2404, 2576, 2749
AxyI CCTNAGG 1 cut(s) 1323
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BalI TGGCCA 1 cut(s) 590
BamHI GGATCC 1 cut(s) 3519
BanI GGYRCC 4 cut(s) 218, 2687, 3276, 3506
BanII GRGCYC 4 cut(s) 562, 1925, 2901, 3463
BbrPI CACGTG 1 cut(s) 2168
BbsI GAAGAC 5 cut(s) 88, 266, 775, 2511, 2975
Bbv12I GWGCWC 2 cut(s) 1304, 3463
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 8 cut(s) 646, 922, 1591, 1594, 1655, 2528, 3050, 3123
BceAI ACGGC 7 cut(s) 143, 159, 219, 232, 2189, 2729, 3544
BcgI CGANNNNNNTGC 6 cut(s) 319, 335, 353, 369, 1353, 1387
BciT130I CCWGG 6 cut(s) 774, 1239, 2185, 2391, 2538, 3512
BciVI GTATCC 1 cut(s) 3581
BclI TGATCA 1 cut(s) 3130
BcnI CCSGG 3 cut(s) 174, 1771, 1772
BcoDI GTCTC 7 cut(s) 31, 94, 467, 1255, 1278, 2268, 2315
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 6 cut(s) 477, 653, 734, 914, 980, 2582
BfmI CTRYAG 3 cut(s) 660, 1544, 1572
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 1352
BfuAI ACCTGC 2 cut(s) 653, 2415
BfuI GTATCC 1 cut(s) 3581
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 1 cut(s) 3115
Bme1390I CCNGG 9 cut(s) 174, 774, 1239, 1771, 1772, 2185, 2391, 2538, 3512
Bme18I GGWCC 5 cut(s) 1669, 2004, 2404, 2576, 2749
BmeT110I CYCGRG 1 cut(s) 1770
BmgT120I GGNCC 9 cut(s) 136, 316, 1669, 2004, 2404, 2534, 2576, 2653, 2749
BmrFI CCNGG 9 cut(s) 174, 774, 1239, 1771, 1772, 2185, 2391, 2538, 3512
BmrI ACTGGG 2 cut(s) 578, 2436
BmuI ACTGGG 2 cut(s) 578, 2436
BpiI GAAGAC 5 cut(s) 88, 266, 775, 2511, 2975
BpmI CTGGAG 8 cut(s) 574, 712, 1302, 1449, 2550, 2910, 2918, 3639
Bpu10I CCTNAGC 2 cut(s) 188, 3669
Bpu14I TTCGAA 1 cut(s) 329
BpuMI CCSGG 3 cut(s) 174, 1771, 1772
BsaAI YACGTR 2 cut(s) 1857, 2168
BsaBI GATNNNNATC 1 cut(s) 2233
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 2 cut(s) 2268, 2315
BsaWI WCCGGW 3 cut(s) 528, 1084, 2690
BsaXI ACNNNNNCTCC 4 cut(s) 885, 915, 2438, 2468
Bsc4I CCNNNNNNNGG 8 cut(s) 413, 947, 1328, 1465, 1740, 2025, 3503, 3516
Bse118I RCCGGY 4 cut(s) 37, 110, 340, 3279
Bse1I ACTGG 7 cut(s) 584, 911, 1171, 2382, 2431, 2533, 3640
Bse21I CCTNAGG 1 cut(s) 1323
Bse8I GATNNNNATC 1 cut(s) 2233
BseAI TCCGGA 2 cut(s) 528, 1084
BseBI CCWGG 6 cut(s) 774, 1239, 2185, 2391, 2538, 3512
BseJI GATNNNNATC 1 cut(s) 2233
BseLI CCNNNNNNNGG 8 cut(s) 413, 947, 1328, 1465, 1740, 2025, 3503, 3516
BseNI ACTGG 7 cut(s) 584, 911, 1171, 2382, 2431, 2533, 3640
BseRI GAGGAG 2 cut(s) 56, 2446
BseX3I CGGCCG 2 cut(s) 342, 3557
BseXI GCAGC 8 cut(s) 646, 922, 1591, 1594, 1655, 2528, 3050, 3123
BseYI CCCAGC 1 cut(s) 1739
BsgI GTGCAG 1 cut(s) 58
Bsh1285I CGRYCG 2 cut(s) 345, 3560
BshNI GGYRCC 4 cut(s) 218, 2687, 3276, 3506
BsiEI CGRYCG 2 cut(s) 345, 3560
BsiHKAI GWGCWC 2 cut(s) 1304, 3463
BsiHKCI CYCGRG 1 cut(s) 1770
BslFI GGGAC 5 cut(s) 248, 489, 1682, 2043, 2417
BslI CCNNNNNNNGG 8 cut(s) 413, 947, 1328, 1465, 1740, 2025, 3503, 3516
BsmAI GTCTC 7 cut(s) 31, 94, 467, 1255, 1278, 2268, 2315
BsmBI CGTCTC 2 cut(s) 31, 94
BsmFI GGGAC 5 cut(s) 248, 489, 1682, 2043, 2417
BsmI GAATGC 1 cut(s) 1549
Bso31I GGTCTC 2 cut(s) 2268, 2315
BsoBI CYCGRG 1 cut(s) 1770
Bsp119I TTCGAA 1 cut(s) 329
Bsp1286I GDGCHC 5 cut(s) 562, 1304, 1925, 2901, 3463
Bsp13I TCCGGA 2 cut(s) 528, 1084
Bsp19I CCATGG 1 cut(s) 291
BspEI TCCGGA 2 cut(s) 528, 1084
BspHI TCATGA 1 cut(s) 2128
BspMAI CTGCAG 1 cut(s) 664
BspMI ACCTGC 2 cut(s) 653, 2415
BspT104I TTCGAA 1 cut(s) 329
BspT107I GGYRCC 4 cut(s) 218, 2687, 3276, 3506
BspTI CTTAAG 1 cut(s) 1352
BspTNI GGTCTC 2 cut(s) 2268, 2315
BsrFI RCCGGY 4 cut(s) 37, 110, 340, 3279
BsrI ACTGG 7 cut(s) 584, 911, 1171, 2382, 2431, 2533, 3640
BssAI RCCGGY 4 cut(s) 37, 110, 340, 3279
BssNAI GTATAC 1 cut(s) 3469
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 3 cut(s) 291, 2019, 2315
Bst1107I GTATAC 1 cut(s) 3469
Bst2UI CCWGG 6 cut(s) 774, 1239, 2185, 2391, 2538, 3512
Bst6I CTCTTC 2 cut(s) 2264, 3368
BstACI GRCGYC 1 cut(s) 54
BstAFI CTTAAG 1 cut(s) 1352
BstAPI GCANNNNNTGC 3 cut(s) 1616, 1889, 3638
BstBAI YACGTR 2 cut(s) 1857, 2168
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 3 cut(s) 342, 2109, 3702
BstDSI CCRYGG 3 cut(s) 291, 2745, 3560
BstEII GGTNACC 3 cut(s) 151, 704, 3476
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstMAI GTCTC 7 cut(s) 31, 94, 467, 1255, 1278, 2268, 2315
BstMCI CGRYCG 2 cut(s) 345, 3560
BstNI CCWGG 6 cut(s) 774, 1239, 2185, 2391, 2538, 3512
BstNSI RCATGY 4 cut(s) 1687, 1807, 3164, 3490
BstPI GGTNACC 3 cut(s) 151, 704, 3476
BstSCI CCNGG 9 cut(s) 172, 772, 1237, 1769, 1770, 2183, 2389, 2536, 3510
BstSFI CTRYAG 3 cut(s) 660, 1544, 1572
BstV1I GCAGC 8 cut(s) 646, 922, 1591, 1594, 1655, 2528, 3050, 3123
BstV2I GAAGAC 5 cut(s) 88, 266, 775, 2511, 2975
BstX2I RGATCY 5 cut(s) 950, 982, 1981, 3115, 3519
BstXI CCANNNNNNTGG 1 cut(s) 2643
BstYI RGATCY 5 cut(s) 950, 982, 1981, 3115, 3519
BstZ17I GTATAC 1 cut(s) 3469
BstZI CGGCCG 2 cut(s) 342, 3557
Bsu36I CCTNAGG 1 cut(s) 1323
BsuI GTATCC 1 cut(s) 3581
BtgI CCRYGG 3 cut(s) 291, 2745, 3560
BtgZI GCGATG 2 cut(s) 1257, 3698
BtsI GCAGTG 1 cut(s) 1519
BtsIMutI CAGTG 8 cut(s) 591, 1128, 1311, 1519, 2526, 3003, 3331, 3478
BveI ACCTGC 2 cut(s) 653, 2415
Cac8I GCNNGC 3 cut(s) 342, 2109, 3702
CaiI CAGNNNCTG 4 cut(s) 124, 195, 1607, 1985
CciI TCATGA 1 cut(s) 2128
CfoI GCGC 1 cut(s) 63
Cfr10I RCCGGY 4 cut(s) 37, 110, 340, 3279
Cfr13I GGNCC 9 cut(s) 136, 316, 1669, 2004, 2404, 2534, 2576, 2653, 2749
Cfr9I CCCGGG 1 cut(s) 1770
CseI GACGC 1 cut(s) 62
CsiI ACCWGGT 1 cut(s) 2389
Csp6I GTAC 6 cut(s) 1152, 1211, 2134, 2918, 3283, 3661
CspCI CAANNNNNGTGG 2 cut(s) 2085, 2120
CviQI GTAC 6 cut(s) 1152, 1211, 2134, 2918, 3283, 3661
DrdI GACNNNNNNGTC 2 cut(s) 1270, 3326
DseDI GACNNNNNNGTC 2 cut(s) 1270, 3326
EaeI YGGCCR 3 cut(s) 342, 588, 3557
EagI CGGCCG 2 cut(s) 342, 3557
Eam1104I CTCTTC 2 cut(s) 2264, 3368
EarI CTCTTC 2 cut(s) 2264, 3368
Ecl136II GAGCTC 1 cut(s) 3461
EclXI CGGCCG 2 cut(s) 342, 3557
Eco130I CCWWGG 3 cut(s) 291, 2019, 2315
Eco147I AGGCCT 1 cut(s) 2314
Eco24I GRGCYC 4 cut(s) 562, 1925, 2901, 3463
Eco31I GGTCTC 2 cut(s) 2268, 2315
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 5 cut(s) 1669, 2004, 2404, 2576, 2749
Eco52I CGGCCG 2 cut(s) 342, 3557
Eco53kI GAGCTC 1 cut(s) 3461
Eco57I CTGAAG 1 cut(s) 82
Eco72I CACGTG 1 cut(s) 2168
Eco81I CCTNAGG 1 cut(s) 1323
Eco88I CYCGRG 1 cut(s) 1770
Eco91I GGTNACC 3 cut(s) 151, 704, 3476
EcoICRI GAGCTC 1 cut(s) 3461
EcoO109I RGGNCCY 4 cut(s) 1669, 2404, 2534, 2653
EcoO65I GGTNACC 3 cut(s) 151, 704, 3476
EcoRI GAATTC 2 cut(s) 1744, 2841
EcoRII CCWGG 6 cut(s) 772, 1237, 2183, 2389, 2536, 3510
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 3 cut(s) 291, 2019, 2315
EcoT22I ATGCAT 3 cut(s) 2236, 2644, 3162
EcoT38I GRGCYC 4 cut(s) 562, 1925, 2901, 3463
ErhI CCWWGG 3 cut(s) 291, 2019, 2315
Esp3I CGTCTC 2 cut(s) 31, 94
FalI AAGNNNNNCTT 8 cut(s) 882, 914, 1103, 1135, 2091, 2123, 2496, 2528
FaqI GGGAC 5 cut(s) 248, 489, 1682, 2043, 2417
FauI CCCGC 1 cut(s) 3634
FauNDI CATATG 6 cut(s) 1103, 1483, 2152, 2296, 2593, 2638
FbaI TGATCA 1 cut(s) 3130
FblI GTMKAC 4 cut(s) 2614, 3050, 3087, 3468
FriOI GRGCYC 4 cut(s) 562, 1925, 2901, 3463
FspBI CTAG 6 cut(s) 477, 653, 734, 914, 980, 2582
GlaI GCGC 1 cut(s) 62
GsaI CCCAGC 1 cut(s) 1743
GsuI CTGGAG 8 cut(s) 574, 712, 1302, 1449, 2550, 2910, 2918, 3639
HaeII RGCGCY 1 cut(s) 64
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 54
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 4 cut(s) 2209, 2992, 3021, 3613
HindII GTYRAC 4 cut(s) 2209, 2992, 3021, 3613
HindIII AAGCTT 3 cut(s) 542, 1835, 2100
Hpy99I CGWCG 2 cut(s) 56, 89
HpyCH4IV ACGT 7 cut(s) 24, 1089, 1311, 1856, 2167, 3078, 3609
HpySE526I ACGT 7 cut(s) 24, 1089, 1311, 1856, 2167, 3078, 3609
Hsp92I GRCGYC 1 cut(s) 54
HspAI GCGC 1 cut(s) 61
KflI GGGWCCC 1 cut(s) 1669
Kpn2I TCCGGA 2 cut(s) 528, 1084
KroI GCCGGC 1 cut(s) 340
KroNI GCCGGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 3130
LmnI GCTCC 7 cut(s) 863, 1074, 1800, 2248, 2305, 2896, 3658
Lsp1109I GCAGC 8 cut(s) 646, 922, 1591, 1594, 1655, 2528, 3050, 3123
MabI ACCWGGT 1 cut(s) 2389
MaeI CTAG 6 cut(s) 477, 653, 734, 914, 980, 2582
MaeII ACGT 7 cut(s) 24, 1089, 1311, 1856, 2167, 3078, 3609
MfeI CAATTG 3 cut(s) 1719, 2462, 3175
MflI RGATCY 5 cut(s) 950, 982, 1981, 3115, 3519
MhlI GDGCHC 5 cut(s) 562, 1304, 1925, 2901, 3463
MlsI TGGCCA 1 cut(s) 590
MluNI TGGCCA 1 cut(s) 590
MlyI GAGTC 5 cut(s) 794, 1829, 2441, 2492, 3056
MmeI TCCRAC 3 cut(s) 72, 111, 665
Mox20I TGGCCA 1 cut(s) 590
Mph1103I ATGCAT 3 cut(s) 2236, 2644, 3162
MroI TCCGGA 2 cut(s) 528, 1084
MroNI GCCGGC 1 cut(s) 340
MroXI GAANNNNTTC 2 cut(s) 894, 2497
MscI TGGCCA 1 cut(s) 590
MseI TTAA 9 cut(s) 686, 837, 968, 1314, 1353, 2043, 3081, 3150, 3342
MslI CAYNNNNRTG 6 cut(s) 383, 1403, 1422, 1688, 2641, 3274
Msp20I TGGCCA 1 cut(s) 590
MspA1I CMGCKG 4 cut(s) 1604, 1646, 2219, 3136
MspCI CTTAAG 1 cut(s) 1352
MspR9I CCNGG 9 cut(s) 174, 774, 1239, 1771, 1772, 2185, 2391, 2538, 3512
MunI CAATTG 3 cut(s) 1719, 2462, 3175
Mva1269I GAATGC 1 cut(s) 1549
MvaI CCWGG 6 cut(s) 774, 1239, 2185, 2391, 2538, 3512
NaeI GCCGGC 1 cut(s) 342
NciI CCSGG 3 cut(s) 174, 1771, 1772
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 6 cut(s) 1103, 1483, 2152, 2296, 2593, 2638
NgoMIV GCCGGC 1 cut(s) 340
NmeAIII GCCGAG 1 cut(s) 274
NsiI ATGCAT 3 cut(s) 2236, 2644, 3162
NspI RCATGY 4 cut(s) 1687, 1807, 3164, 3490
NspV TTCGAA 1 cut(s) 329
OliI CACNNNNGTG 2 cut(s) 383, 1403
PagI TCATGA 1 cut(s) 2128
PaqCI CACCTGC 1 cut(s) 653
PceI AGGCCT 1 cut(s) 2314
PciI ACATGT 1 cut(s) 3486
PcsI WCGNNNNNNNCGW 1 cut(s) 2742
PctI GAATGC 1 cut(s) 1549
PdiI GCCGGC 1 cut(s) 342
PdmI GAANNNNTTC 2 cut(s) 894, 2497
PflMI CCANNNNNTGG 2 cut(s) 947, 3503
PleI GAGTC 5 cut(s) 794, 1828, 2441, 2491, 3055
PmaCI CACGTG 1 cut(s) 2168
PmlI CACGTG 1 cut(s) 2168
PpsI GAGTC 5 cut(s) 794, 1828, 2441, 2491, 3055
Ppu21I YACGTR 2 cut(s) 1857, 2168
PpuMI RGGWCCY 2 cut(s) 1669, 2404
PscI ACATGT 1 cut(s) 3486
PshBI ATTAAT 2 cut(s) 837, 3150
Psp124BI GAGCTC 1 cut(s) 3463
Psp1406I AACGTT 1 cut(s) 3609
Psp5II RGGWCCY 2 cut(s) 1669, 2404
Psp6I CCWGG 6 cut(s) 772, 1237, 2183, 2389, 2536, 3510
PspCI CACGTG 1 cut(s) 2168
PspEI GGTNACC 3 cut(s) 151, 704, 3476
PspFI CCCAGC 1 cut(s) 1739
PspGI CCWGG 6 cut(s) 772, 1237, 2183, 2389, 2536, 3510
PspPI GGNCC 9 cut(s) 136, 316, 1669, 2004, 2404, 2534, 2576, 2653, 2749
PspPPI RGGWCCY 2 cut(s) 1669, 2404
PstI CTGCAG 1 cut(s) 664
PstNI CAGNNNCTG 4 cut(s) 124, 195, 1607, 1985
PsuI RGATCY 5 cut(s) 950, 982, 1981, 3115, 3519
PvuII CAGCTG 3 cut(s) 1604, 1646, 3136
RsaI GTAC 6 cut(s) 1153, 1212, 2135, 2919, 3284, 3662
RsaNI GTAC 6 cut(s) 1152, 1211, 2134, 2918, 3283, 3661
RseI CAYNNNNRTG 6 cut(s) 383, 1403, 1422, 1688, 2641, 3274
SacI GAGCTC 1 cut(s) 3463
SaqAI TTAA 9 cut(s) 686, 837, 968, 1314, 1353, 2043, 3081, 3150, 3342
Sau96I GGNCC 9 cut(s) 136, 316, 1669, 2004, 2404, 2534, 2576, 2653, 2749
SchI GAGTC 5 cut(s) 794, 1829, 2441, 2492, 3056
ScrFI CCNGG 9 cut(s) 174, 774, 1239, 1771, 1772, 2185, 2391, 2538, 3512
SduI GDGCHC 5 cut(s) 562, 1304, 1925, 2901, 3463
SexAI ACCWGGT 1 cut(s) 2389
SfcI CTRYAG 3 cut(s) 660, 1544, 1572
SfuI TTCGAA 1 cut(s) 329
SinI GGWCC 5 cut(s) 1669, 2004, 2404, 2576, 2749
SmaI CCCGGG 1 cut(s) 1772
SmiMI CAYNNNNRTG 6 cut(s) 383, 1403, 1422, 1688, 2641, 3274
SmlI CTYRAG 1 cut(s) 1352
SmoI CTYRAG 1 cut(s) 1352
SpeI ACTAGT 1 cut(s) 733
SseBI AGGCCT 1 cut(s) 2314
SspI AATATT 2 cut(s) 2798, 3154
SspMI CTAG 6 cut(s) 477, 653, 734, 914, 980, 2582
SstI GAGCTC 1 cut(s) 3463
StuI AGGCCT 1 cut(s) 2314
StyD4I CCNGG 9 cut(s) 172, 772, 1237, 1769, 1770, 2183, 2389, 2536, 3510
StyI CCWWGG 3 cut(s) 291, 2019, 2315
TaiI ACGT 7 cut(s) 27, 1092, 1314, 1859, 2170, 3081, 3612
TaqI TCGA 5 cut(s) 329, 448, 2489, 2914, 3457
TatI WGTACW 3 cut(s) 2133, 2917, 3660
TauI GCSGC 5 cut(s) 15, 245, 248, 3069, 3559
Tru1I TTAA 9 cut(s) 686, 837, 968, 1314, 1353, 2043, 3081, 3150, 3342
Tru9I TTAA 9 cut(s) 686, 837, 968, 1314, 1353, 2043, 3081, 3150, 3342
TscAI CASTG 8 cut(s) 591, 1135, 1311, 1519, 2533, 3003, 3331, 3478
TseI GCWGC 8 cut(s) 659, 910, 1604, 1607, 1643, 2541, 3063, 3136
TspGWI ACGGA 4 cut(s) 82, 338, 1996, 3512
TspMI CCCGGG 1 cut(s) 1770
TspRI CASTG 8 cut(s) 591, 1135, 1311, 1519, 2533, 3003, 3331, 3478
Van91I CCANNNNNTGG 2 cut(s) 947, 3503
Vha464I CTTAAG 1 cut(s) 1352
VpaK11BI GGWCC 5 cut(s) 1669, 2004, 2404, 2576, 2749
VspI ATTAAT 2 cut(s) 837, 3150
XbaI TCTAGA 1 cut(s) 979
XceI RCATGY 4 cut(s) 1687, 1807, 3164, 3490
XcmI CCANNNNNNNNNTGG 3 cut(s) 1998, 2942, 3181
XmaI CCCGGG 1 cut(s) 1770
XmiI GTMKAC 4 cut(s) 2614, 3050, 3087, 3468
XmnI GAANNNNTTC 2 cut(s) 894, 2497
XspI CTAG 6 cut(s) 477, 653, 734, 914, 980, 2582
Zsp2I ATGCAT 3 cut(s) 2236, 2644, 3162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.