RchiOBHm_Chr4g0401591

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009414
Physical Location & Seq
Forward (+)
19815128 .. 19824300
9173 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37349

Sequence Viewer

Length: 2421 bp
ATGATTGGGGGCGGCAAGAGCTACGTCTCAGCCCCACCGGCCTTCTCCAACGATGCCAAGCGCCTCCTCGTCTGCACCGGCACCGCCGTCTCCGTCTTCAGCACCTCCACCGGCTTACAGATACTGTCGTTGGAGGGCCACACGGCGTTGGTCACCGCCGTCATAGTGGTTCCCGGTAACAAAGCCCTCAGCTTCTGCTGGACGGCCTCGCTTGACGGCACCGTTAGGTATTGGGACTTTGCCGCCGCCGAGTTGATGAAGACCATTGATATCAAAATGCCTATTTACTCCATGGTTATTCCGGCATTGTTGTTGGGCCAAACGGATTCGAAGGTGATGCCGGCGGATGCTTTTGCTTATTTGTCTGTTGAAAACACCACCCAAGACAATGCACCCAAGGCTTTGCGTGGGCAGATTCGCAAGTGTAGTTTGACTGAGTCTCGATTGGCTGGTGTTATTCTCGCAGAGACAAGGAAACCAGAAGTTATATCTATCAGTCCCTCAGGAAAATTCTTTGGCATCCGGAACAAGCGGAAGCTTCATATCTGGAGAGCCCCTGCTAAAGATTCTAAACTCCCAGTGGCCAAGATTACACTACACCATACAAGAAATTTTACACTTCTTGCACTTCATCCAACAAAGAGGCTAGTTGCTGCAGGTGATACATCTGGGAGAATTCTAATCTGGAGAGGGTTTGGTAACCGTAAATTTTCGGATGGTGACAAACTAGTAAATCAGGGATCAATGAATGATGAGGAAGGAAGACCTGGGATCAGGGGAGATGATGATGCTGACTCTTGCACCACTTGGCATTGGCATCCTACTGGAATTAATCTCCTGCGTTTTTCTTCTGATGGAGCGTATTTATATTCAGGTGGAAAGGAAGGAGTTCTTGTGGACTGGCAGCTAGACACAGGGAAAAAGAAATTTTTACCAAGAATTGGATCTCCACTTCTATATTTAACTGATTCTCTAGATCCTTCACTTTCTGCTCTATCTTGTGCAGATAATCAAATTCACTTGCTAAAAATGCCCTCAATGGAAATCACAAAGTCTATTTCAGGGATCAAGCTCCCTTTTTCTTCTCCGGACGTATATGAAGGCATATGCAGCAAGTTTGCCTTTGATGACACTGATGGGTTAGTTGCCTTGCGTACAGAGAACTATCGCATCCAGTTTTACAGCTTGTTTGATGACCGTGAAATTTCTGAGGTTCAAGTCTGTGAGAGAAACCATCAACCGGGTGATGAAGTCTCTGTAATAGTGACTTTGGTGTCTCTGTCTGGAGATGGCTCTATGATGAGCACAGTGGAAGTTAATCTTCCTGAGGATGGAATAGGTGGTCTTGTGTGTCTTAAGTTCTGGGCATCAGATTCGCAGAACAAAAATTTCAACTTGTCCACCATTGTGTATCAACCTCACAGGGATGCTGGAGTTTCTGCTCTTGCTTTTCATCCTACCCGTCAAATGGTTGTCAGTTCATCATATGGTGGGGATTTCAAGATTTGGGTTTGCAGTGACGAGATTCAGCAGAAACAAGATACACTACAGCATTCCGGGTGGATGTGCTATGCTGTAGGTTCATACAAGAAAAAGCCAATGACAGCTGCTGCATTTTCTGCCGATGGTTCTGTTCTGGCTGTTGCAGCTGAAACTGTAATCACACTATGGGACCCTGTGAACAACATGCTTGTGGCTGTGCTTGGAGAGATGCAGATGCCAATTGTGACCCTCTCCTTTGCTGGGAATTCGGACTATCTTTTGTCTATCTCCCGGGGTTCAAAACCACAGTTATCTGTGTGGAGCATGTCAAAGCTGTCTGAGTCTTGGTCATACAAGCTTGACATAGAAGATGTTACGTGTGCGAAAGATTCATCATCATTTGCAGTTCTTGCCCATTTGCCAAAATCATCTACTTTTATGGGCTCTAATGAACCAATTTTTCAAGGAAAAGATGGGGTAATCTTATTATTCAATGCAACAGATCCTGTTCCATTGGCTATGTGGTCCGTAAGGAAGGCCAAGGGAGGGGGACTTGCTTTCCTTAAAGGGAACTCCTTTCAACAACAAAGTATAGCGGATGGAAAATCACCACAAGCTTTGCTTGCCTATATAAATGCCGATCATGAGTACATCCTCTTTGATCCATATGACTACGATGCACGTGAACTTAGAATGACCAGGGGGAAGGATCGTGTTGCCGTTGACGAAACAGCGCGATATGGATATGCATCTATCTATGGGGAACTACCGCAATTTGAGATGAAGAGAGACCAGACATTGTTAGCCCCATATGCTCCATCACAAAGGCCTTGGGAGACCATTTTTAGTGGGTCATCACATAATCTTCTACCCCTACCTAAATTGTGTTTAGAGTTTATGGAATCACTGCTAGAAAGGAGGACTACCACAGTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
WD40

Protein Analysis

806

Amino Acids

88.56

Weight (kDa)

6.35

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR75_1st PF23869 15 - 236 1.4e-24 WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st PF24817 15 - 226 2.9e-08 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 268 - 337 1.8e-12 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR75_2nd PF23769 386 - 639 1.2e-34 WD repeat-containing protein 75 second beta-propeller
WD40_Prp19 PF24814 453 - 615 1.1e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 478 - 610 1.1e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 478 - 614 1.3e-08 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 647
AasI GACNNNNNNGTC 1 cut(s) 1273
Acc36I ACCTGC 1 cut(s) 647
AccB1I GGYRCC 2 cut(s) 80, 218
AccB7I CCANNNNNTGG 1 cut(s) 941
AccII CGCG 1 cut(s) 2218
AccIII TCCGGA 2 cut(s) 522, 1087
AciI CCGC 9 cut(s) 12, 84, 156, 243, 246, 344, 532, 2078, 2252
AclWI GGATC 8 cut(s) 748, 779, 952, 971, 1073, 1979, 2138, 2199
AcoI YGGCCR 1 cut(s) 582
AcsI RAATTY 9 cut(s) 509, 610, 675, 707, 926, 1014, 1203, 1387, 1747
AcuI CTGAAG 1 cut(s) 82
AcvI CACGTG 1 cut(s) 2165
AfaI GTAC 2 cut(s) 1156, 2132
AfiI CCNNNNNNNGG 6 cut(s) 941, 1240, 1331, 1468, 1743, 2028
AflII CTTAAG 1 cut(s) 1355
AflIII ACRYGT 1 cut(s) 1859
AgsI TTSAA 8 cut(s) 371, 1217, 1393, 1501, 1782, 1946, 1975, 2063
AhlI ACTAGT 1 cut(s) 727
AjnI CCWGG 2 cut(s) 766, 2180
AleI CACNNNNGTG 1 cut(s) 1406
Alw21I GWGCWC 1 cut(s) 1307
Alw26I GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2265, 2312
AlwI GGATC 8 cut(s) 748, 779, 952, 971, 1073, 1979, 2138, 2199
AlwNI CAGNNNCTG 4 cut(s) 124, 195, 1610, 1988
Ama87I CYCGRG 1 cut(s) 1773
Aor13HI TCCGGA 2 cut(s) 522, 1087
AoxI GGCC 7 cut(s) 39, 136, 204, 316, 582, 2019, 2309
ApeKI GCWGC 6 cut(s) 653, 904, 1110, 1607, 1610, 1646
ApoI RAATTY 9 cut(s) 509, 610, 675, 707, 926, 1014, 1203, 1387, 1747
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 888
AspLEI GCGC 2 cut(s) 63, 2218
AspS9I GGNCC 4 cut(s) 136, 316, 1672, 2007
AsuC2I CCSGG 5 cut(s) 174, 1242, 1558, 1774, 1775
AsuHPI GGTGA 6 cut(s) 145, 346, 671, 731, 1256, 2082
AsuII TTCGAA 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 1773
AvaII GGWCC 2 cut(s) 1672, 2007
AxyI CCTNAGG 2 cut(s) 502, 1326
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BalI TGGCCA 1 cut(s) 584
BanI GGYRCC 2 cut(s) 80, 218
BanII GRGCYC 2 cut(s) 556, 1928
BbrPI CACGTG 1 cut(s) 2165
BbsI GAAGAC 3 cut(s) 88, 266, 769
Bbv12I GWGCWC 1 cut(s) 1307
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 6 cut(s) 640, 916, 1122, 1594, 1597, 1658
BceAI ACGGC 6 cut(s) 71, 143, 159, 219, 232, 2186
BcgI CGANNNNNNTGC 4 cut(s) 319, 353, 1356, 1390
BciT130I CCWGG 2 cut(s) 768, 2182
BcnI CCSGG 5 cut(s) 174, 1242, 1558, 1774, 1775
BcoDI GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2265, 2312
BcuI ACTAGT 1 cut(s) 727
BfaI CTAG 5 cut(s) 647, 728, 908, 974, 2393
BfmI CTRYAG 3 cut(s) 654, 1547, 1575
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 1355
BfuAI ACCTGC 1 cut(s) 647
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
BlsI GCNGC 9 cut(s) 14, 244, 247, 655, 906, 1112, 1609, 1612, 1648
Bme1390I CCNGG 7 cut(s) 174, 768, 1242, 1558, 1774, 1775, 2182
Bme18I GGWCC 2 cut(s) 1672, 2007
BmeT110I CYCGRG 1 cut(s) 1773
BmgT120I GGNCC 4 cut(s) 136, 316, 1672, 2007
BmiI GGNNCC 5 cut(s) 82, 171, 220, 1673, 1674
BmrFI CCNGG 7 cut(s) 174, 768, 1242, 1558, 1774, 1775, 2182
BmrI ACTGGG 1 cut(s) 572
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 3 cut(s) 88, 266, 769
BpmI CTGGAG 4 cut(s) 568, 706, 1305, 1452
Bpu10I CCTNAGC 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 329
BpuMI CCSGG 5 cut(s) 174, 1242, 1558, 1774, 1775
BsaAI YACGTR 2 cut(s) 1860, 2165
BsaBI GATNNNNATC 1 cut(s) 2230
BsaI GGTCTC 2 cut(s) 2265, 2312
BsaJI CCNNGG 8 cut(s) 291, 396, 767, 1773, 1774, 2022, 2181, 2312
BsaWI WCCGGW 2 cut(s) 522, 1087
BsaXI ACNNNNNCTCC 2 cut(s) 879, 909
Bsc4I CCNNNNNNNGG 6 cut(s) 941, 1240, 1331, 1468, 1743, 2028
Bse118I RCCGGY 4 cut(s) 37, 77, 110, 340
Bse1I ACTGG 4 cut(s) 578, 829, 905, 1174
Bse21I CCTNAGG 2 cut(s) 502, 1326
Bse8I GATNNNNATC 1 cut(s) 2230
BseAI TCCGGA 2 cut(s) 522, 1087
BseBI CCWGG 2 cut(s) 768, 2182
BseDI CCNNGG 8 cut(s) 291, 396, 767, 1773, 1774, 2022, 2181, 2312
BseJI GATNNNNATC 1 cut(s) 2230
BseLI CCNNNNNNNGG 6 cut(s) 941, 1240, 1331, 1468, 1743, 2028
BseMII CTCAG 7 cut(s) 42, 202, 426, 516, 1200, 1317, 1812
BseNI ACTGG 4 cut(s) 578, 829, 905, 1174
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 6 cut(s) 640, 916, 1122, 1594, 1597, 1658
BseYI CCCAGC 1 cut(s) 1742
BsgI GTGCAG 2 cut(s) 58, 1023
Bsh1236I CGCG 1 cut(s) 2218
BshFI GGCC 7 cut(s) 41, 138, 206, 318, 584, 2021, 2311
BshNI GGYRCC 2 cut(s) 80, 218
BsiHKAI GWGCWC 1 cut(s) 1307
BsiHKCI CYCGRG 1 cut(s) 1773
BslFI GGGAC 4 cut(s) 248, 483, 1685, 2046
BslI CCNNNNNNNGG 6 cut(s) 941, 1240, 1331, 1468, 1743, 2028
BsmAI GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2265, 2312
BsmBI CGTCTC 2 cut(s) 31, 94
BsmFI GGGAC 4 cut(s) 248, 483, 1685, 2046
BsmI GAATGC 1 cut(s) 1552
BsnI GGCC 7 cut(s) 41, 138, 206, 318, 584, 2021, 2311
Bso31I GGTCTC 2 cut(s) 2265, 2312
BsoBI CYCGRG 1 cut(s) 1773
Bsp119I TTCGAA 1 cut(s) 329
Bsp1286I GDGCHC 3 cut(s) 556, 1307, 1928
Bsp13I TCCGGA 2 cut(s) 522, 1087
Bsp143I GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2122, 2143, 2191
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 9 cut(s) 12, 84, 156, 243, 246, 344, 532, 2078, 2252
BspANI GGCC 7 cut(s) 41, 138, 206, 318, 584, 2021, 2311
BspCNI CTCAG 7 cut(s) 41, 201, 427, 515, 1201, 1318, 1813
BspEI TCCGGA 2 cut(s) 522, 1087
BspFNI CGCG 1 cut(s) 2218
BspHI TCATGA 1 cut(s) 2125
BspLI GGNNCC 5 cut(s) 82, 171, 220, 1673, 1674
BspMAI CTGCAG 1 cut(s) 658
BspMI ACCTGC 1 cut(s) 647
BspPI GGATC 8 cut(s) 748, 779, 952, 971, 1073, 1979, 2138, 2199
BspT104I TTCGAA 1 cut(s) 329
BspT107I GGYRCC 2 cut(s) 80, 218
BspTI CTTAAG 1 cut(s) 1355
BspTNI GGTCTC 2 cut(s) 2265, 2312
BsrFI RCCGGY 4 cut(s) 37, 77, 110, 340
BsrI ACTGG 4 cut(s) 578, 829, 905, 1174
BssAI RCCGGY 4 cut(s) 37, 77, 110, 340
BssECI CCNNGG 8 cut(s) 291, 396, 767, 1773, 1774, 2022, 2181, 2312
BssMI GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2122, 2143, 2191
BssT1I CCWWGG 4 cut(s) 291, 396, 2022, 2312
Bst2UI CCWGG 2 cut(s) 768, 2182
Bst4CI ACNGT 8 cut(s) 126, 223, 704, 1199, 1309, 1657, 1791, 2413
Bst6I CTCTTC 1 cut(s) 2261
BstAFI CTTAAG 1 cut(s) 1355
BstAPI GCANNNNNTGC 2 cut(s) 1619, 1892
BstBAI YACGTR 2 cut(s) 1860, 2165
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 342, 2106
BstDEI CTNAG 8 cut(s) 28, 188, 435, 502, 1209, 1326, 1821, 2171
BstDSI CCRYGG 1 cut(s) 291
BstEII GGTNACC 2 cut(s) 151, 698
BstFNI CGCG 1 cut(s) 2218
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 2 cut(s) 63, 2218
BstKTI GATC 9 cut(s) 743, 774, 947, 979, 1068, 1987, 2125, 2146, 2194
BstMAI GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2265, 2312
BstMBI GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2122, 2143, 2191
BstNI CCWGG 2 cut(s) 768, 2182
BstNSI RCATGY 2 cut(s) 1690, 1810
BstPI GGTNACC 2 cut(s) 151, 698
BstSCI CCNGG 7 cut(s) 172, 766, 1240, 1556, 1772, 1773, 2180
BstSFI CTRYAG 3 cut(s) 654, 1547, 1575
BstUI CGCG 1 cut(s) 2218
BstV1I GCAGC 6 cut(s) 640, 916, 1122, 1594, 1597, 1658
BstV2I GAAGAC 3 cut(s) 88, 266, 769
BstX2I RGATCY 3 cut(s) 944, 976, 1984
BstYI RGATCY 3 cut(s) 944, 976, 1984
Bsu36I CCTNAGG 2 cut(s) 502, 1326
BsuRI GGCC 7 cut(s) 41, 138, 206, 318, 584, 2021, 2311
BtgI CCRYGG 1 cut(s) 291
BtsI GCAGTG 2 cut(s) 1522, 2387
BtsIMutI CAGTG 5 cut(s) 585, 1131, 1314, 1522, 2387
BveI ACCTGC 1 cut(s) 647
Cac8I GCNNGC 2 cut(s) 342, 2106
CaiI CAGNNNCTG 4 cut(s) 124, 195, 1610, 1988
CciI TCATGA 1 cut(s) 2125
CfoI GCGC 2 cut(s) 63, 2218
Cfr10I RCCGGY 4 cut(s) 37, 77, 110, 340
Cfr13I GGNCC 4 cut(s) 136, 316, 1672, 2007
Cfr9I CCCGGG 1 cut(s) 1773
Csp6I GTAC 2 cut(s) 1155, 2131
CspCI CAANNNNNGTGG 2 cut(s) 2082, 2117
CviAII CATG 4 cut(s) 292, 1687, 1807, 2126
CviQI GTAC 2 cut(s) 1155, 2131
DdeI CTNAG 8 cut(s) 28, 188, 435, 502, 1209, 1326, 1821, 2171
DpnI GATC 9 cut(s) 742, 773, 946, 978, 1067, 1986, 2124, 2145, 2193
DpnII GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2122, 2143, 2191
DrdI GACNNNNNNGTC 1 cut(s) 1273
DseDI GACNNNNNNGTC 1 cut(s) 1273
EaeI YGGCCR 1 cut(s) 582
Eam1104I CTCTTC 1 cut(s) 2261
EarI CTCTTC 1 cut(s) 2261
EciI GGCGGA 1 cut(s) 359
Eco130I CCWWGG 4 cut(s) 291, 396, 2022, 2312
Eco147I AGGCCT 1 cut(s) 2311
Eco24I GRGCYC 2 cut(s) 556, 1928
Eco31I GGTCTC 2 cut(s) 2265, 2312
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 1672, 2007
Eco57I CTGAAG 1 cut(s) 82
Eco72I CACGTG 1 cut(s) 2165
Eco81I CCTNAGG 2 cut(s) 502, 1326
Eco88I CYCGRG 1 cut(s) 1773
Eco91I GGTNACC 2 cut(s) 151, 698
EcoO109I RGGNCCY 1 cut(s) 1672
EcoO65I GGTNACC 2 cut(s) 151, 698
EcoRI GAATTC 2 cut(s) 675, 1747
EcoRII CCWGG 2 cut(s) 766, 2180
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 4 cut(s) 291, 396, 2022, 2312
EcoT22I ATGCAT 1 cut(s) 2233
EcoT38I GRGCYC 2 cut(s) 556, 1928
ErhI CCWWGG 4 cut(s) 291, 396, 2022, 2312
Esp3I CGTCTC 2 cut(s) 31, 94
FaeI CATG 4 cut(s) 295, 1690, 1810, 2129
FalI AAGNNNNNCTT 8 cut(s) 876, 908, 1106, 1138, 1305, 1337, 2088, 2120
FaqI GGGAC 4 cut(s) 248, 483, 1685, 2046
FatI CATG 4 cut(s) 291, 1686, 1806, 2125
FauNDI CATATG 4 cut(s) 1106, 1486, 2149, 2293
Fnu4HI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
FriOI GRGCYC 2 cut(s) 556, 1928
Fsp4HI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
FspBI CTAG 5 cut(s) 647, 728, 908, 974, 2393
GlaI GCGC 2 cut(s) 62, 2217
GluI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
GsaI CCCAGC 1 cut(s) 1746
GsuI CTGGAG 4 cut(s) 568, 706, 1305, 1452
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 7 cut(s) 41, 138, 206, 318, 584, 2021, 2311
HhaI GCGC 2 cut(s) 63, 2218
Hin1II CATG 4 cut(s) 295, 1690, 1810, 2129
Hin6I GCGC 2 cut(s) 61, 2216
HinP1I GCGC 2 cut(s) 61, 2216
HincII GTYRAC 1 cut(s) 2206
HindII GTYRAC 1 cut(s) 2206
HindIII AAGCTT 3 cut(s) 536, 1838, 2097
HphI GGTGA 6 cut(s) 145, 346, 671, 731, 1256, 2082
Hpy166II GTNNAC 5 cut(s) 898, 1401, 1681, 2168, 2206
Hpy188I TCNGA 6 cut(s) 715, 853, 1210, 1372, 1753, 1822
Hpy8I GTNNAC 5 cut(s) 898, 1401, 1681, 2168, 2206
HpyAV CCTTC 8 cut(s) 52, 325, 752, 878, 990, 1094, 2011, 2182
HpyCH4III ACNGT 8 cut(s) 126, 223, 704, 1199, 1309, 1657, 1791, 2413
HpyCH4IV ACGT 4 cut(s) 24, 1092, 1859, 2164
HpyF3I CTNAG 8 cut(s) 28, 188, 435, 502, 1209, 1326, 1821, 2171
HpySE526I ACGT 4 cut(s) 24, 1092, 1859, 2164
Hsp92II CATG 4 cut(s) 295, 1690, 1810, 2129
HspAI GCGC 2 cut(s) 61, 2216
KflI GGGWCCC 1 cut(s) 1672
Kpn2I TCCGGA 2 cut(s) 522, 1087
KroI GCCGGC 1 cut(s) 340
KroNI GCCGGC 1 cut(s) 342
Kzo9I GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2122, 2143, 2191
LmnI GCTCC 4 cut(s) 857, 1077, 1803, 2302
Lsp1109I GCAGC 6 cut(s) 640, 916, 1122, 1594, 1597, 1658
MaeI CTAG 5 cut(s) 647, 728, 908, 974, 2393
MaeII ACGT 4 cut(s) 24, 1092, 1859, 2164
MaeIII GTNAC 8 cut(s) 151, 176, 698, 719, 1264, 1517, 1726, 1855
MalI GATC 9 cut(s) 742, 773, 946, 978, 1067, 1986, 2124, 2145, 2193
MboI GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2122, 2143, 2191
MboII GAAGA 9 cut(s) 88, 271, 774, 840, 1074, 1313, 1862, 2278, 2339
MfeI CAATTG 1 cut(s) 1722
MflI RGATCY 3 cut(s) 944, 976, 1984
MhlI GDGCHC 3 cut(s) 556, 1307, 1928
MlsI TGGCCA 1 cut(s) 584
MluNI TGGCCA 1 cut(s) 584
MlyI GAGTC 3 cut(s) 446, 788, 1832
MmeI TCCRAC 3 cut(s) 72, 111, 659
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 1 cut(s) 2233
MroI TCCGGA 2 cut(s) 522, 1087
MroNI GCCGGC 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 888
MscI TGGCCA 1 cut(s) 584
MseI TTAA 5 cut(s) 831, 962, 1317, 1356, 2046
MslI CAYNNNNRTG 3 cut(s) 1406, 1425, 1691
Msp20I TGGCCA 1 cut(s) 584
MspA1I CMGCKG 2 cut(s) 1607, 1649
MspCI CTTAAG 1 cut(s) 1355
MspR9I CCNGG 7 cut(s) 174, 768, 1242, 1558, 1774, 1775, 2182
MunI CAATTG 1 cut(s) 1722
Mva1269I GAATGC 1 cut(s) 1552
MvaI CCWGG 2 cut(s) 768, 2182
MvnI CGCG 1 cut(s) 2218
NaeI GCCGGC 1 cut(s) 342
NciI CCSGG 5 cut(s) 174, 1242, 1558, 1774, 1775
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 4 cut(s) 1106, 1486, 2149, 2293
NdeII GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2122, 2143, 2191
NgoMIV GCCGGC 1 cut(s) 340
NlaIII CATG 4 cut(s) 295, 1690, 1810, 2129
NlaIV GGNNCC 5 cut(s) 82, 171, 220, 1673, 1674
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 5 cut(s) 151, 719, 1264, 1517, 1726
NsiI ATGCAT 1 cut(s) 2233
NspI RCATGY 2 cut(s) 1690, 1810
NspV TTCGAA 1 cut(s) 329
OliI CACNNNNGTG 1 cut(s) 1406
PagI TCATGA 1 cut(s) 2125
PaqCI CACCTGC 1 cut(s) 647
PceI AGGCCT 1 cut(s) 2311
PctI GAATGC 1 cut(s) 1552
PdiI GCCGGC 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 888
PfeI GAWTC 8 cut(s) 326, 415, 566, 968, 1373, 1525, 1871, 2384
PflFI GACNNNGTC 1 cut(s) 436
PflMI CCANNNNNTGG 1 cut(s) 941
PkrI GCNGC 9 cut(s) 14, 244, 247, 655, 906, 1112, 1609, 1612, 1648
PleI GAGTC 3 cut(s) 445, 788, 1831
PmaCI CACGTG 1 cut(s) 2165
PmlI CACGTG 1 cut(s) 2165
PpsI GAGTC 3 cut(s) 445, 788, 1831
Ppu21I YACGTR 2 cut(s) 1860, 2165
PpuMI RGGWCCY 1 cut(s) 1672
PshBI ATTAAT 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 1672
Psp6I CCWGG 2 cut(s) 766, 2180
PspCI CACGTG 1 cut(s) 2165
PspEI GGTNACC 2 cut(s) 151, 698
PspFI CCCAGC 1 cut(s) 1742
PspGI CCWGG 2 cut(s) 766, 2180
PspN4I GGNNCC 5 cut(s) 82, 171, 220, 1673, 1674
PspPI GGNCC 4 cut(s) 136, 316, 1672, 2007
PspPPI RGGWCCY 1 cut(s) 1672
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 4 cut(s) 124, 195, 1610, 1988
PsuI RGATCY 3 cut(s) 944, 976, 1984
PsyI GACNNNGTC 1 cut(s) 436
PvuII CAGCTG 2 cut(s) 1607, 1649
RsaI GTAC 2 cut(s) 1156, 2132
RsaNI GTAC 2 cut(s) 1155, 2131
RseI CAYNNNNRTG 3 cut(s) 1406, 1425, 1691
SaqAI TTAA 5 cut(s) 831, 962, 1317, 1356, 2046
SatI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
Sau3AI GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2122, 2143, 2191
Sau96I GGNCC 4 cut(s) 136, 316, 1672, 2007
SchI GAGTC 3 cut(s) 446, 788, 1832
ScrFI CCNGG 7 cut(s) 174, 768, 1242, 1558, 1774, 1775, 2182
SduI GDGCHC 3 cut(s) 556, 1307, 1928
SfcI CTRYAG 3 cut(s) 654, 1547, 1575
SfuI TTCGAA 1 cut(s) 329
SinI GGWCC 2 cut(s) 1672, 2007
SmaI CCCGGG 1 cut(s) 1775
SmiMI CAYNNNNRTG 3 cut(s) 1406, 1425, 1691
SmlI CTYRAG 1 cut(s) 1355
SmoI CTYRAG 1 cut(s) 1355
SpeI ACTAGT 1 cut(s) 727
SseBI AGGCCT 1 cut(s) 2311
SsiI CCGC 9 cut(s) 12, 84, 156, 243, 246, 344, 532, 2078, 2252
SspMI CTAG 5 cut(s) 647, 728, 908, 974, 2393
StuI AGGCCT 1 cut(s) 2311
StyD4I CCNGG 7 cut(s) 172, 766, 1240, 1556, 1772, 1773, 2180
StyI CCWWGG 4 cut(s) 291, 396, 2022, 2312
TaaI ACNGT 8 cut(s) 126, 223, 704, 1199, 1309, 1657, 1791, 2413
TaiI ACGT 4 cut(s) 27, 1095, 1862, 2167
TaqI TCGA 2 cut(s) 329, 442
TatI WGTACW 1 cut(s) 2130
TauI GCSGC 3 cut(s) 15, 245, 248
TfiI GAWTC 8 cut(s) 326, 415, 566, 968, 1373, 1525, 1871, 2384
Tru1I TTAA 5 cut(s) 831, 962, 1317, 1356, 2046
Tru9I TTAA 5 cut(s) 831, 962, 1317, 1356, 2046
TscAI CASTG 5 cut(s) 585, 1138, 1314, 1522, 2394
TseFI GTSAC 5 cut(s) 151, 719, 1264, 1517, 1726
TseI GCWGC 6 cut(s) 653, 904, 1110, 1607, 1610, 1646
Tsp45I GTSAC 5 cut(s) 151, 719, 1264, 1517, 1726
TspGWI ACGGA 3 cut(s) 82, 338, 1999
TspMI CCCGGG 1 cut(s) 1773
TspRI CASTG 5 cut(s) 585, 1138, 1314, 1522, 2394
Tth111I GACNNNGTC 1 cut(s) 436
Van91I CCANNNNNTGG 1 cut(s) 941
Vha464I CTTAAG 1 cut(s) 1355
VpaK11BI GGWCC 2 cut(s) 1672, 2007
VspI ATTAAT 1 cut(s) 831
XapI RAATTY 9 cut(s) 509, 610, 675, 707, 926, 1014, 1203, 1387, 1747
XbaI TCTAGA 1 cut(s) 973
XceI RCATGY 2 cut(s) 1690, 1810
XcmI CCANNNNNNNNNTGG 1 cut(s) 2001
XmaI CCCGGG 1 cut(s) 1773
XmnI GAANNNNTTC 1 cut(s) 888
XspI CTAG 5 cut(s) 647, 728, 908, 974, 2393
Zsp2I ATGCAT 1 cut(s) 2233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.