Rh4BG101900

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009414
Physical Location & Seq
Forward (+)
17602397 .. 17611475
9079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG101900.1

Sequence Viewer

Length: 2031 bp
ATGATTGGGGGCGGCAAGAGCTACGTCTCAGCCCCACCGGCCTTCTCCAACGACGCCAAGCGCCTCCTCCTCTGCACCGGCACCGCCGTCTCCGTCTTCAGCACCTCCACCGGCTTACAGATACTGTCGTTGGAGGGCCACACGGCGTTGGTCACCGCCGTCATAGTGGTTCCCGGTAACAAAGCCCTCAGCTTCTGCTGGACGGCCTCGCTTGACGGCACCGTTAGGTATTGGGACTTTGCCGCCGCCGAGTTGATGAAGACCATTGATATCAAAATGCCTATTTACTCCATGGTTATTCCGGCATTGTTGTTGGGCCAAACGGATTCGAAGGTGATGCCAACCGACGCTTTTGCTTATTTGTCTGTTGAAAACACCACCCAAGTGCAAGACAATGCACCCAAGGCTTTGCGCGGGCAGATTCGCAAGTGTAATTTGACTGAGTCTCGATTGGCTGGTGTTATTCTCGCAGAGACTAGAAAACCAGAAGTTATATCTATTAGTCCCTCAGGAAAATTCTTTGGCATCCGGAACAAACGGAAGCTTCATATCTGGAGAGCCCCTGCTAAAGATTCTAAACTCCCAGTGGCCAAGATTACGCTACACCATACAAGAAATTTTACACTTCTTGCATTTCATCCAACAAAGAGGCTAGTTGCTGCAGGTGATACATCTGGGAGAATTCTAATCTGGAGAGGGTTTGGTAACCGTAAATTTTCGGATGGTGACAAACTAGTAAATCGGGGATTAATGAATGATGAGGAAGGAAGACCTGGGATCAGGGGAGATGATGATGCTGACTCTTGCACCACTTGGCATTGGCATCCTACTGGAATTAATCTCCTGCGTTTTTCTTCTGATGGAGCATATTTATATTCAGGTGGAAAGGAAGGAGTTCTTGTGGACTGGCAGCTAGACACAGGGAAAAAGAAATTTTTACCAAGAATTGGATCTCCACTTCTATATTTAACAGATTCTCTAGATCCTTCACTTTCTGCTCTATCTTGTGCAGATAATCAAATTCACTTGCTAAAAATGCCCTCAATGGAAATCACAAAGTCTATTTCAGGGATCAAGCTCCCTTTTTCTTCTCCGGACGTATATGAAGGCATATGCAGCAAGTTTGCCTTCGATGACACTGATGGGTTAGTTGCCTTGCGTACAGAGAACTATCGCATCCAGTTTTACAGCTTGTTTGATGACCGTGAAATTTCTGAGGTTCAAGTCTGTCAGAGAAACCATCAACCAGGCGATGAAGTCTCTGTAATGGTGACTTTGGTGTCTCTGTCTGGAGATGGCTCTATGATGAGCACGGTGGAAGTTAATCTTCCTGAGGATGGAATAGGTGGTCTTGTGTGTCTTAAGTTCTGGGCATCAGATTCACAGAACAAAAATTTCAACTTGTCGACCATTGTGTATGAACCTCACAGGGATGCTGGAGTTTCTGCTCTTGCTTTTCATCCTACCCGTCAAATGGTTGTCAGTTCATCATATGGTGGGGATTTCAAGATTTGGGTTTGCAGTGACGAGATTCAGCAGAAACAAGATGCACTACAGCATTCTGGGTGGATGTGCCATGCTGTAGGTTCATACAAGAAAAAGCCAATGACAGCTGCTGCATTTTCTGCCGATGGTTCTGTTCTGGCTGTTGCAGCTGAAACTGTAATCACACTATGGGACCCTGTGAACAACATGCTTGTGGCTGTGCTTGGAGAGATCCAGGTGCTAATTGTGACCCTCTCCTTTGCTGGGAATTCGGACTATCTTTTGTCTATCTCTCGGGGTTCAAAACCACAGTTATCTGTGTGGAGCATGTCAAAGTTGTCTGAGTCTTGGTCATACAAGCTTGACATAGAAGATGTTACGTGTGCGAAAGATTCATCATCATTTGCAGTTCTTGCCCTTTTGCCAAAATCATCTACTTTTATGGGCTCTAATGAACTAATTTTTAAAGGAAAAGATGGGGTAATCTTATTATTCAATGCAACAGATCCTGTTCCATTGGCTATGTGGTCCGTAAGGAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
WD40

Protein Analysis

676

Amino Acids

73.89

Weight (kDa)

7.18

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR75_1st PF23869 15 - 238 9.1e-25 WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st PF24817 21 - 228 2.1e-09 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 270 - 339 1.4e-12 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR75_2nd PF23769 388 - 641 1.7e-35 WD repeat-containing protein 75 second beta-propeller
WD40_Prp19 PF24814 455 - 617 1.9e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 477 - 612 9.3e-07 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 478 - 607 3.1e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 481 - 619 3.5e-07 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 653
AasI GACNNNNNNGTC 1 cut(s) 1279
Acc36I ACCTGC 1 cut(s) 653
AccB1I GGYRCC 2 cut(s) 80, 218
AccB7I CCANNNNNTGG 1 cut(s) 947
AccI GTMKAC 1 cut(s) 1406
AccII CGCG 1 cut(s) 414
AccIII TCCGGA 2 cut(s) 528, 1093
AciI CCGC 6 cut(s) 12, 84, 156, 243, 246, 414
AclWI GGATC 6 cut(s) 785, 958, 977, 1079, 1711, 1985
AcoI YGGCCR 1 cut(s) 588
AcsI RAATTY 9 cut(s) 515, 616, 681, 713, 932, 1020, 1209, 1393, 1753
AcuI CTGAAG 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 1 cut(s) 1162
AfiI CCNNNNNNNGG 4 cut(s) 947, 1337, 1474, 1749
AflII CTTAAG 1 cut(s) 1361
AflIII ACRYGT 1 cut(s) 1865
AgsI TTSAA 6 cut(s) 371, 1223, 1399, 1507, 1788, 1981
AhlI ACTAGT 1 cut(s) 733
AjnI CCWGG 3 cut(s) 772, 1246, 1719
AleI CACNNNNGTG 1 cut(s) 383
AluBI AGCT 9 cut(s) 21, 192, 544, 913, 1078, 1191, 1613, 1655, 1846
AluI AGCT 9 cut(s) 21, 192, 544, 913, 1078, 1191, 1613, 1655, 1846
Alw21I GWGCWC 1 cut(s) 1313
Alw26I GTCTC 6 cut(s) 31, 94, 450, 467, 1264, 1287
AlwI GGATC 6 cut(s) 785, 958, 977, 1079, 1711, 1985
AlwNI CAGNNNCTG 4 cut(s) 124, 195, 1616, 1994
Ama87I CYCGRG 1 cut(s) 1779
Aor13HI TCCGGA 2 cut(s) 528, 1093
AoxI GGCC 5 cut(s) 39, 136, 204, 316, 588
ApeKI GCWGC 6 cut(s) 659, 910, 1116, 1613, 1616, 1652
ApoI RAATTY 9 cut(s) 515, 616, 681, 713, 932, 1020, 1209, 1393, 1753
AseI ATTAAT 2 cut(s) 749, 837
Asp700I GAANNNNTTC 1 cut(s) 894
AspLEI GCGC 2 cut(s) 63, 414
AspS9I GGNCC 4 cut(s) 136, 316, 1678, 2013
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 5 cut(s) 145, 346, 677, 737, 1282
AsuII TTCGAA 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 1779
AvaII GGWCC 2 cut(s) 1678, 2013
AxyI CCTNAGG 2 cut(s) 508, 1332
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BalI TGGCCA 1 cut(s) 590
BanI GGYRCC 2 cut(s) 80, 218
BanII GRGCYC 2 cut(s) 562, 1934
BbsI GAAGAC 3 cut(s) 88, 266, 775
Bbv12I GWGCWC 1 cut(s) 1313
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 6 cut(s) 646, 922, 1128, 1600, 1603, 1664
BccI CCATC 8 cut(s) 716, 854, 1136, 1248, 1289, 1331, 1625, 1955
BceAI ACGGC 5 cut(s) 71, 143, 159, 219, 232
BcgI CGANNNNNNTGC 4 cut(s) 319, 335, 353, 369
BciT130I CCWGG 3 cut(s) 774, 1248, 1721
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 6 cut(s) 31, 94, 450, 467, 1264, 1287
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 5 cut(s) 477, 653, 734, 914, 980
BfmI CTRYAG 3 cut(s) 660, 1553, 1581
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 1361
BfuAI ACCTGC 1 cut(s) 653
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 9 cut(s) 13, 243, 246, 660, 911, 1117, 1614, 1617, 1653
BlsI GCNGC 9 cut(s) 14, 244, 247, 661, 912, 1118, 1615, 1618, 1654
Bme1390I CCNGG 4 cut(s) 174, 774, 1248, 1721
Bme18I GGWCC 2 cut(s) 1678, 2013
BmeT110I CYCGRG 1 cut(s) 1779
BmgT120I GGNCC 4 cut(s) 136, 316, 1678, 2013
BmiI GGNNCC 5 cut(s) 82, 171, 220, 1679, 1680
BmrFI CCNGG 4 cut(s) 174, 774, 1248, 1721
BmrI ACTGGG 1 cut(s) 578
BmsI GCATC 8 cut(s) 327, 534, 784, 832, 1185, 1382, 1423, 1537
BmuI ACTGGG 1 cut(s) 578
BpiI GAAGAC 3 cut(s) 88, 266, 775
BpmI CTGGAG 4 cut(s) 574, 712, 1311, 1458
Bpu10I CCTNAGC 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 174
BsaAI YACGTR 1 cut(s) 1866
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 291, 402, 773
BsaWI WCCGGW 2 cut(s) 528, 1093
BsaXI ACNNNNNCTCC 2 cut(s) 885, 915
Bsc4I CCNNNNNNNGG 4 cut(s) 947, 1337, 1474, 1749
Bse118I RCCGGY 3 cut(s) 37, 77, 110
Bse1I ACTGG 4 cut(s) 584, 835, 911, 1180
Bse21I CCTNAGG 2 cut(s) 508, 1332
BseAI TCCGGA 2 cut(s) 528, 1093
BseBI CCWGG 3 cut(s) 774, 1248, 1721
BseDI CCNNGG 3 cut(s) 291, 402, 773
BseGI GGATG 9 cut(s) 525, 637, 727, 823, 1176, 1342, 1438, 1459, 1575
BseLI CCNNNNNNNGG 4 cut(s) 947, 1337, 1474, 1749
BseMII CTCAG 7 cut(s) 42, 202, 432, 522, 1206, 1323, 1818
BseNI ACTGG 4 cut(s) 584, 835, 911, 1180
BseRI GAGGAG 2 cut(s) 56, 59
BseXI GCAGC 6 cut(s) 646, 922, 1128, 1600, 1603, 1664
BseYI CCCAGC 1 cut(s) 1748
BsgI GTGCAG 2 cut(s) 58, 1029
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 5 cut(s) 41, 138, 206, 318, 590
BshNI GGYRCC 2 cut(s) 80, 218
BsiHKAI GWGCWC 1 cut(s) 1313
BsiHKCI CYCGRG 1 cut(s) 1779
BsiSI CCGG 7 cut(s) 38, 78, 111, 174, 302, 529, 1094
BslFI GGGAC 3 cut(s) 248, 489, 1691
BslI CCNNNNNNNGG 4 cut(s) 947, 1337, 1474, 1749
BsmAI GTCTC 6 cut(s) 31, 94, 450, 467, 1264, 1287
BsmBI CGTCTC 2 cut(s) 31, 94
BsmFI GGGAC 3 cut(s) 248, 489, 1691
BsmI GAATGC 1 cut(s) 1558
BsnI GGCC 5 cut(s) 41, 138, 206, 318, 590
BsoBI CYCGRG 1 cut(s) 1779
Bsp119I TTCGAA 1 cut(s) 329
Bsp1286I GDGCHC 3 cut(s) 562, 1313, 1934
Bsp13I TCCGGA 2 cut(s) 528, 1093
Bsp143I GATC 6 cut(s) 777, 950, 982, 1071, 1716, 1990
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 6 cut(s) 12, 84, 156, 243, 246, 414
BspANI GGCC 5 cut(s) 41, 138, 206, 318, 590
BspCNI CTCAG 7 cut(s) 41, 201, 433, 521, 1207, 1324, 1819
BspEI TCCGGA 2 cut(s) 528, 1093
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 5 cut(s) 82, 171, 220, 1679, 1680
BspMAI CTGCAG 1 cut(s) 664
BspMI ACCTGC 1 cut(s) 653
BspPI GGATC 6 cut(s) 785, 958, 977, 1079, 1711, 1985
BspT104I TTCGAA 1 cut(s) 329
BspT107I GGYRCC 2 cut(s) 80, 218
BspTI CTTAAG 1 cut(s) 1361
BsrFI RCCGGY 3 cut(s) 37, 77, 110
BsrI ACTGG 4 cut(s) 584, 835, 911, 1180
BssAI RCCGGY 3 cut(s) 37, 77, 110
BssECI CCNNGG 3 cut(s) 291, 402, 773
BssMI GATC 6 cut(s) 777, 950, 982, 1071, 1716, 1990
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 2 cut(s) 291, 402
Bst2UI CCWGG 3 cut(s) 774, 1248, 1721
Bst4CI ACNGT 7 cut(s) 126, 223, 710, 1205, 1315, 1663, 1797
BstACI GRCGYC 1 cut(s) 54
BstAFI CTTAAG 1 cut(s) 1361
BstAPI GCANNNNNTGC 2 cut(s) 1625, 1898
BstBAI YACGTR 1 cut(s) 1866
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 416
BstDEI CTNAG 7 cut(s) 28, 188, 441, 508, 1215, 1332, 1827
BstDSI CCRYGG 1 cut(s) 291
BstEII GGTNACC 2 cut(s) 151, 704
BstF5I GGATG 9 cut(s) 525, 637, 727, 823, 1176, 1342, 1438, 1459, 1575
BstFNI CGCG 1 cut(s) 414
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 2 cut(s) 63, 414
BstKTI GATC 6 cut(s) 780, 953, 985, 1074, 1719, 1993
BstMAI GTCTC 6 cut(s) 31, 94, 450, 467, 1264, 1287
BstMBI GATC 6 cut(s) 777, 950, 982, 1071, 1716, 1990
BstMWI GCNNNNNNNGC 8 cut(s) 18, 38, 404, 1036, 1116, 1625, 1652, 1898
BstNI CCWGG 3 cut(s) 774, 1248, 1721
BstNSI RCATGY 2 cut(s) 1696, 1816
BstPI GGTNACC 2 cut(s) 151, 704
BstSCI CCNGG 4 cut(s) 172, 772, 1246, 1719
BstSFI CTRYAG 3 cut(s) 660, 1553, 1581
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 6 cut(s) 646, 922, 1128, 1600, 1603, 1664
BstV2I GAAGAC 3 cut(s) 88, 266, 775
BstX2I RGATCY 4 cut(s) 950, 982, 1716, 1990
BstYI RGATCY 4 cut(s) 950, 982, 1716, 1990
Bsu36I CCTNAGG 2 cut(s) 508, 1332
BsuRI GGCC 5 cut(s) 41, 138, 206, 318, 590
BtgI CCRYGG 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 1266
BtsCI GGATG 9 cut(s) 525, 637, 727, 823, 1176, 1342, 1438, 1459, 1575
BtsI GCAGTG 1 cut(s) 1528
BtsIMutI CAGTG 3 cut(s) 591, 1137, 1528
BveI ACCTGC 1 cut(s) 653
Cac8I GCNNGC 1 cut(s) 416
CaiI CAGNNNCTG 4 cut(s) 124, 195, 1616, 1994
CfoI GCGC 2 cut(s) 63, 414
Cfr10I RCCGGY 3 cut(s) 37, 77, 110
Cfr13I GGNCC 4 cut(s) 136, 316, 1678, 2013
CseI GACGC 2 cut(s) 62, 356
Csp6I GTAC 1 cut(s) 1161
CviAII CATG 4 cut(s) 292, 1577, 1693, 1813
CviQI GTAC 1 cut(s) 1161
DdeI CTNAG 7 cut(s) 28, 188, 441, 508, 1215, 1332, 1827
DpnI GATC 6 cut(s) 779, 952, 984, 1073, 1718, 1992
DpnII GATC 6 cut(s) 777, 950, 982, 1071, 1716, 1990
DraI TTTAAA 1 cut(s) 1951
DrdI GACNNNNNNGTC 1 cut(s) 1279
DseDI GACNNNNNNGTC 1 cut(s) 1279
EaeI YGGCCR 1 cut(s) 588
Eco130I CCWWGG 2 cut(s) 291, 402
Eco24I GRGCYC 2 cut(s) 562, 1934
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 1678, 2013
Eco57I CTGAAG 1 cut(s) 82
Eco81I CCTNAGG 2 cut(s) 508, 1332
Eco88I CYCGRG 1 cut(s) 1779
Eco91I GGTNACC 2 cut(s) 151, 704
EcoO109I RGGNCCY 1 cut(s) 1678
EcoO65I GGTNACC 2 cut(s) 151, 704
EcoRI GAATTC 2 cut(s) 681, 1753
EcoRII CCWGG 3 cut(s) 772, 1246, 1719
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 2 cut(s) 291, 402
EcoT38I GRGCYC 2 cut(s) 562, 1934
ErhI CCWWGG 2 cut(s) 291, 402
Esp3I CGTCTC 2 cut(s) 31, 94
FaeI CATG 4 cut(s) 295, 1580, 1696, 1816
FalI AAGNNNNNCTT 6 cut(s) 882, 914, 1112, 1144, 1311, 1343
FaqI GGGAC 3 cut(s) 248, 489, 1691
FatI CATG 4 cut(s) 291, 1576, 1692, 1812
FauI CCCGC 1 cut(s) 407
FauNDI CATATG 2 cut(s) 1112, 1492
FblI GTMKAC 1 cut(s) 1406
Fnu4HI GCNGC 9 cut(s) 13, 243, 246, 660, 911, 1117, 1614, 1617, 1653
FokI GGATG 9 cut(s) 512, 624, 734, 810, 1163, 1349, 1445, 1446, 1582
FriOI GRGCYC 2 cut(s) 562, 1934
Fsp4HI GCNGC 9 cut(s) 13, 243, 246, 660, 911, 1117, 1614, 1617, 1653
FspBI CTAG 5 cut(s) 477, 653, 734, 914, 980
GlaI GCGC 2 cut(s) 62, 413
GluI GCNGC 9 cut(s) 13, 243, 246, 660, 911, 1117, 1614, 1617, 1653
GsaI CCCAGC 1 cut(s) 1752
GsuI CTGGAG 4 cut(s) 574, 712, 1311, 1458
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 5 cut(s) 41, 138, 206, 318, 590
HapII CCGG 7 cut(s) 38, 78, 111, 174, 302, 529, 1094
HgaI GACGC 2 cut(s) 62, 356
HhaI GCGC 2 cut(s) 63, 414
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 4 cut(s) 295, 1580, 1696, 1816
Hin6I GCGC 2 cut(s) 61, 412
HinP1I GCGC 2 cut(s) 61, 412
HincII GTYRAC 1 cut(s) 1407
HindII GTYRAC 1 cut(s) 1407
HindIII AAGCTT 2 cut(s) 542, 1844
HpaII CCGG 7 cut(s) 38, 78, 111, 174, 302, 529, 1094
HphI GGTGA 5 cut(s) 145, 346, 677, 737, 1282
Hpy166II GTNNAC 3 cut(s) 904, 1407, 1687
Hpy188I TCNGA 7 cut(s) 721, 859, 1216, 1233, 1378, 1759, 1828
Hpy8I GTNNAC 3 cut(s) 904, 1407, 1687
Hpy99I CGWCG 2 cut(s) 56, 350
HpyAV CCTTC 8 cut(s) 52, 325, 758, 884, 996, 1100, 1138, 2017
HpyCH4III ACNGT 7 cut(s) 126, 223, 710, 1205, 1315, 1663, 1797
HpyCH4IV ACGT 3 cut(s) 24, 1098, 1865
HpyF10VI GCNNNNNNNGC 8 cut(s) 18, 38, 404, 1036, 1116, 1625, 1652, 1898
HpyF3I CTNAG 7 cut(s) 28, 188, 441, 508, 1215, 1332, 1827
HpySE526I ACGT 3 cut(s) 24, 1098, 1865
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 4 cut(s) 295, 1580, 1696, 1816
HspAI GCGC 2 cut(s) 61, 412
KflI GGGWCCC 1 cut(s) 1678
Kpn2I TCCGGA 2 cut(s) 528, 1093
Kzo9I GATC 6 cut(s) 777, 950, 982, 1071, 1716, 1990
LmnI GCTCC 3 cut(s) 863, 1083, 1809
Lsp1109I GCAGC 6 cut(s) 646, 922, 1128, 1600, 1603, 1664
LweI GCATC 8 cut(s) 327, 534, 784, 832, 1185, 1382, 1423, 1537
MaeI CTAG 5 cut(s) 477, 653, 734, 914, 980
MaeII ACGT 3 cut(s) 24, 1098, 1865
MaeIII GTNAC 8 cut(s) 151, 176, 704, 725, 1270, 1523, 1732, 1861
MalI GATC 6 cut(s) 779, 952, 984, 1073, 1718, 1992
MboI GATC 6 cut(s) 777, 950, 982, 1071, 1716, 1990
MboII GAAGA 7 cut(s) 88, 271, 780, 846, 1080, 1319, 1868
MflI RGATCY 4 cut(s) 950, 982, 1716, 1990
MhlI GDGCHC 3 cut(s) 562, 1313, 1934
MlsI TGGCCA 1 cut(s) 590
MluNI TGGCCA 1 cut(s) 590
MlyI GAGTC 3 cut(s) 452, 794, 1838
MmeI TCCRAC 3 cut(s) 72, 111, 665
Mox20I TGGCCA 1 cut(s) 590
MroI TCCGGA 2 cut(s) 528, 1093
MroXI GAANNNNTTC 1 cut(s) 894
MscI TGGCCA 1 cut(s) 590
MseI TTAA 6 cut(s) 749, 837, 968, 1323, 1362, 1950
MslI CAYNNNNRTG 3 cut(s) 383, 1431, 1697
Msp20I TGGCCA 1 cut(s) 590
MspA1I CMGCKG 2 cut(s) 1613, 1655
MspCI CTTAAG 1 cut(s) 1361
MspI CCGG 7 cut(s) 38, 78, 111, 174, 302, 529, 1094
MspR9I CCNGG 4 cut(s) 174, 774, 1248, 1721
Mva1269I GAATGC 1 cut(s) 1558
MvaI CCWGG 3 cut(s) 774, 1248, 1721
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 8 cut(s) 18, 38, 404, 1036, 1116, 1625, 1652, 1898
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 2 cut(s) 1112, 1492
NdeII GATC 6 cut(s) 777, 950, 982, 1071, 1716, 1990
NlaIII CATG 4 cut(s) 295, 1580, 1696, 1816
NlaIV GGNNCC 5 cut(s) 82, 171, 220, 1679, 1680
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 5 cut(s) 151, 725, 1270, 1523, 1732
NspI RCATGY 2 cut(s) 1696, 1816
NspV TTCGAA 1 cut(s) 329
OliI CACNNNNGTG 1 cut(s) 383
PaqCI CACCTGC 1 cut(s) 653
PctI GAATGC 1 cut(s) 1558
PdmI GAANNNNTTC 1 cut(s) 894
PfeI GAWTC 7 cut(s) 326, 421, 572, 974, 1379, 1531, 1877
PflFI GACNNNGTC 1 cut(s) 442
PflMI CCANNNNNTGG 1 cut(s) 947
PkrI GCNGC 9 cut(s) 14, 244, 247, 661, 912, 1118, 1615, 1618, 1654
PleI GAGTC 3 cut(s) 451, 794, 1837
PpsI GAGTC 3 cut(s) 451, 794, 1837
Ppu21I YACGTR 1 cut(s) 1866
PpuMI RGGWCCY 1 cut(s) 1678
PshBI ATTAAT 2 cut(s) 749, 837
Psp5II RGGWCCY 1 cut(s) 1678
Psp6I CCWGG 3 cut(s) 772, 1246, 1719
PspEI GGTNACC 2 cut(s) 151, 704
PspFI CCCAGC 1 cut(s) 1748
PspGI CCWGG 3 cut(s) 772, 1246, 1719
PspN4I GGNNCC 5 cut(s) 82, 171, 220, 1679, 1680
PspPI GGNCC 4 cut(s) 136, 316, 1678, 2013
PspPPI RGGWCCY 1 cut(s) 1678
PstI CTGCAG 1 cut(s) 664
PstNI CAGNNNCTG 4 cut(s) 124, 195, 1616, 1994
PsuI RGATCY 4 cut(s) 950, 982, 1716, 1990
PsyI GACNNNGTC 1 cut(s) 442
PvuII CAGCTG 2 cut(s) 1613, 1655
RsaI GTAC 1 cut(s) 1162
RsaNI GTAC 1 cut(s) 1161
RseI CAYNNNNRTG 3 cut(s) 383, 1431, 1697
SalI GTCGAC 1 cut(s) 1405
SaqAI TTAA 6 cut(s) 749, 837, 968, 1323, 1362, 1950
SatI GCNGC 9 cut(s) 13, 243, 246, 660, 911, 1117, 1614, 1617, 1653
Sau3AI GATC 6 cut(s) 777, 950, 982, 1071, 1716, 1990
Sau96I GGNCC 4 cut(s) 136, 316, 1678, 2013
SchI GAGTC 3 cut(s) 452, 794, 1838
ScrFI CCNGG 4 cut(s) 174, 774, 1248, 1721
SduI GDGCHC 3 cut(s) 562, 1313, 1934
SfaNI GCATC 8 cut(s) 327, 534, 784, 832, 1185, 1382, 1423, 1537
SfcI CTRYAG 3 cut(s) 660, 1553, 1581
SfuI TTCGAA 1 cut(s) 329
SinI GGWCC 2 cut(s) 1678, 2013
SmiMI CAYNNNNRTG 3 cut(s) 383, 1431, 1697
SmlI CTYRAG 1 cut(s) 1361
SmoI CTYRAG 1 cut(s) 1361
SpeI ACTAGT 1 cut(s) 733
SsiI CCGC 6 cut(s) 12, 84, 156, 243, 246, 414
SspMI CTAG 5 cut(s) 477, 653, 734, 914, 980
StyD4I CCNGG 4 cut(s) 172, 772, 1246, 1719
StyI CCWWGG 2 cut(s) 291, 402
TaaI ACNGT 7 cut(s) 126, 223, 710, 1205, 1315, 1663, 1797
TaiI ACGT 3 cut(s) 27, 1101, 1868
TaqI TCGA 4 cut(s) 329, 448, 1131, 1406
TauI GCSGC 3 cut(s) 15, 245, 248
TfiI GAWTC 7 cut(s) 326, 421, 572, 974, 1379, 1531, 1877
Tru1I TTAA 6 cut(s) 749, 837, 968, 1323, 1362, 1950
Tru9I TTAA 6 cut(s) 749, 837, 968, 1323, 1362, 1950
TscAI CASTG 3 cut(s) 591, 1144, 1528
TseFI GTSAC 5 cut(s) 151, 725, 1270, 1523, 1732
TseI GCWGC 6 cut(s) 659, 910, 1116, 1613, 1616, 1652
Tsp45I GTSAC 5 cut(s) 151, 725, 1270, 1523, 1732
TspGWI ACGGA 4 cut(s) 82, 338, 553, 2005
TspRI CASTG 3 cut(s) 591, 1144, 1528
Tth111I GACNNNGTC 1 cut(s) 442
Van91I CCANNNNNTGG 1 cut(s) 947
Vha464I CTTAAG 1 cut(s) 1361
VpaK11BI GGWCC 2 cut(s) 1678, 2013
VspI ATTAAT 2 cut(s) 749, 837
XapI RAATTY 9 cut(s) 515, 616, 681, 713, 932, 1020, 1209, 1393, 1753
XbaI TCTAGA 1 cut(s) 979
XceI RCATGY 2 cut(s) 1696, 1816
XcmI CCANNNNNNNNNTGG 1 cut(s) 2007
XmiI GTMKAC 1 cut(s) 1406
XmnI GAANNNNTTC 1 cut(s) 894
XspI CTAG 5 cut(s) 477, 653, 734, 914, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.