Rh4DG099200

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009414
Physical Location & Seq
Forward (+)
15078269 .. 15086536
8268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG099200.1

Sequence Viewer

Length: 2427 bp
ATGATTGGGGGCGGCAAGAGCTACGTCTCAGCCCCACCGGCCTTCTCCAACGACGCCAAGCGCCTCCTCGTCTGCACCGGCACCGTCGTCTCCGTCTTCAGCACCTCCACCGGCTTACAGATACTGTCGTTGGAGGGCCACACGGCGTTGGTCACCGCCGTCATAGTGGTTCCCGGTAACAAAGCCCTCAGCTTCTGCTGGACGGCCTCGCTTGACGGCACCGTTAGGTATTGGGACTTTGCCGCCGCCGAGTTGATGAAGACCATTGATATCAAGATGCCTATTTACTCCATGGTTATTCCGGCATTGTTGTTGGGCCAAACGGATTCGAAGGTGATGCCGGCCGATGCTTTTGCTTATTTGTCTGTTGAAAACACCACCCAAGACAATGCACCCAAGGCTTTGAGTGGGCAGATTCGCAAGTGTAATTTGACTGAGTCTCGATTGGCTGGTGTTATTCTCGTAGAGACTAGAAAACCAGAAGTTATATCTATCAGTCCCTCAGGAAAATTCTTTGGCATCTGGAACAAGCGGAAGCTTCATATCTGGAGAGCCCCTGCTAAAGATTCTAAACTCCCAGTGGCCAAGATTACGCTACACCATACAAGAAATTTTACACTTCTTGCATTTCATCCAACAAAGAGGCTAGTTGCTGCAGGTGATACATCTGGGAGAATTCTAATCTGGAGAGGGTTTGGTAACCATAAATTTTCGGATGGTGACAAACTAGTAAATCGGGGATCAATGAATGATGAGGAAGGAAGACCTGGGATCAGGGGAGATGATGATGCTGACTCTTGCACCACTTGGCATTGGCATCCTACTGGAATTAATCTCCTGCGTTTTTCTTCTGATGGAGCGTATTTATATTCAGGTGGAAAGGAAGGAGTTCTCGTGGACTGGCAGCTAGACACAGGGAAAAAGAAATTTTTACCAAGAATTGGATCTCCACTTCTATATTTAACTGATTCTCTAGATCCTTCACTTTCTGCTCTATCTTGTGCAGATAATCAAATTCACTTGCTAAAAATGCCCTCAATGGAAATCACAAAGTCTATTTCAGGGATCAAGCTCCCTTTTTCTTCTCCAGACGTATATGAAGGCATATGCAGCAAGTTTGCCTTCGATGACACTGATGGGTTAGTTGCCTTGCGTACAGAGAACTATCGCATCCAGTTTTACAGCTTGTTTGATGACCGTGAAATTTCTGAGGTTCAAGTCTGTGAGAGAAACCATCAACCAGGCGATGAAGTCTCTGTAATAGTGACTTTGGTGTCTCTGTCTGGAGATGGCTCTATGATGAGCACAGTGGAAGTTAATCTTCCTGAGGATGGAATAGGTGGTCTTGTGTGTCTTAAGTTCTGGGCATCAGATTCGCAGAACAAAAATTTCAACATGTCCACCATTGTGTATGAACCTCACAGGGATGCTGGAGTTTCTGCTCTTGCTTTTCATCCTACCCGTCAAATGGTTGTCAGTTCATCATATGGTGGGGATTTCAAGATTTGGGTTTGCAGTGACGAGATTCAGCAGAAACAAGATACACTACAGCATTCTGGGTGGATGTGCCATGCTGTAGGTTCATACAAGAAAAAGCCAATGACAGCTGCTGCATTTTCTGCCGATGGTTCTGTTCTGGCTGTTGCAGCTGAAACTGTAATCACACTATGGGACCCTGTGAACAACATGCTTGTGGCTGTGCTTGGAGAGATGCAGATGCCAATTTTGACCCTCTCCTTTGCTGGGAATTCGGACTATCTTTTGTCTATCTCCCGGGGTTCAAAACCACAGTTATCTGTGTGGAGCATGTCAAAGCTGTCTGAGTCTTGGTCATACAAGCTTGACATAGAAGATGTTACGTGTGCGAAAGATTCATCATCATTTGCAGTTCTTGCCCTTTTGCCAAAATCATCTACTTTTATGGGCTCTAATGAACCAATTTTTCAAGGAAAAGATGGGGTAATCTTATTATTCAATGCAACAGATCCTGTTCCATTGGCTATGTGGTCCGTAAGGAAGGCCAAGGGAGGGGGACTTGCTTTCCTTAAAGGGAACTCACCTTCCTTTCAACAACAAAGTATAGCGGATGGAAAATCACCACAAGTTTTGCTTGCCTATATAAATGCAGATCATGAGTACATCCTCTTTGATCCATATGACTACGATGCACGTGAACTTAGTATGACCAGGGGGAAGGATCGTGTTGCCGTTGACGAAACAGCGCAATATGGATATGCATCTATCTATGGGGAGCTACCGCAATTTGAGATGAAGAGAGACCAGACCTTGTTAGCCCCATATGCTCCATCACAAAGGCCTTGGGAGACCATTTTTAGTGGGTCATCACATAATCTTCTACCCCTACCTAAATTGTGTTTAGAGTTTATGGAATCACTGCTAGAAAGGAGGACTACCACAGTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
WD40

Protein Analysis

808

Amino Acids

88.75

Weight (kDa)

5.95

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR75_1st PF23869 15 - 236 1.3e-24 WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st PF24817 15 - 226 1.3e-08 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 268 - 337 1.8e-12 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR75_2nd PF23769 386 - 639 2.4e-34 WD repeat-containing protein 75 second beta-propeller
WD40_Prp19 PF24814 453 - 615 1.3e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 473 - 610 5.4e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 479 - 613 2.7e-08 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 647
AasI GACNNNNNNGTC 1 cut(s) 1273
Acc36I ACCTGC 1 cut(s) 647
AccB1I GGYRCC 2 cut(s) 80, 218
AccB7I CCANNNNNTGG 1 cut(s) 941
AciI CCGC 7 cut(s) 12, 156, 243, 246, 532, 2084, 2258
AclWI GGATC 8 cut(s) 748, 779, 952, 971, 1073, 1979, 2144, 2205
AcoI YGGCCR 2 cut(s) 342, 582
AcsI RAATTY 9 cut(s) 509, 610, 675, 707, 926, 1014, 1203, 1387, 1747
AcuI CTGAAG 1 cut(s) 82
AcvI CACGTG 1 cut(s) 2171
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 1156, 2138
AfiI CCNNNNNNNGG 5 cut(s) 941, 1331, 1468, 1743, 2028
AflII CTTAAG 1 cut(s) 1355
AflIII ACRYGT 2 cut(s) 1395, 1859
AgsI TTSAA 8 cut(s) 371, 1217, 1393, 1501, 1782, 1946, 1975, 2069
AhlI ACTAGT 1 cut(s) 727
AjnI CCWGG 3 cut(s) 766, 1240, 2186
AleI CACNNNNGTG 1 cut(s) 1406
AloI GAACNNNNNNTCC 2 cut(s) 2045, 2077
Alw21I GWGCWC 1 cut(s) 1307
Alw26I GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2271, 2318
AlwI GGATC 8 cut(s) 748, 779, 952, 971, 1073, 1979, 2144, 2205
AlwNI CAGNNNCTG 4 cut(s) 124, 195, 1610, 1988
Ama87I CYCGRG 1 cut(s) 1773
AoxI GGCC 8 cut(s) 39, 136, 204, 316, 342, 582, 2019, 2315
ApeKI GCWGC 6 cut(s) 653, 904, 1110, 1607, 1610, 1646
ApoI RAATTY 9 cut(s) 509, 610, 675, 707, 926, 1014, 1203, 1387, 1747
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 888
AspLEI GCGC 2 cut(s) 63, 2224
AspS9I GGNCC 4 cut(s) 136, 316, 1672, 2007
AsuC2I CCSGG 3 cut(s) 174, 1774, 1775
AsuHPI GGTGA 6 cut(s) 145, 346, 671, 731, 2049, 2088
AsuII TTCGAA 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 1773
AvaII GGWCC 2 cut(s) 1672, 2007
AxyI CCTNAGG 2 cut(s) 502, 1326
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BalI TGGCCA 1 cut(s) 584
BanI GGYRCC 2 cut(s) 80, 218
BanII GRGCYC 2 cut(s) 556, 1928
BauI CACGAG 1 cut(s) 893
BbrPI CACGTG 1 cut(s) 2171
BbsI GAAGAC 3 cut(s) 88, 266, 769
Bbv12I GWGCWC 1 cut(s) 1307
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 6 cut(s) 640, 916, 1122, 1594, 1597, 1658
BceAI ACGGC 5 cut(s) 143, 159, 219, 232, 2192
BcgI CGANNNNNNTGC 6 cut(s) 319, 335, 353, 369, 1356, 1390
BciT130I CCWGG 3 cut(s) 768, 1242, 2188
BcnI CCSGG 3 cut(s) 174, 1774, 1775
BcoDI GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2271, 2318
BcuI ACTAGT 1 cut(s) 727
BfaI CTAG 6 cut(s) 471, 647, 728, 908, 974, 2399
BfmI CTRYAG 3 cut(s) 654, 1547, 1575
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 1355
BfuAI ACCTGC 1 cut(s) 647
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
BlsI GCNGC 9 cut(s) 14, 244, 247, 655, 906, 1112, 1609, 1612, 1648
Bme1390I CCNGG 6 cut(s) 174, 768, 1242, 1774, 1775, 2188
Bme18I GGWCC 2 cut(s) 1672, 2007
BmeT110I CYCGRG 1 cut(s) 1773
BmgT120I GGNCC 4 cut(s) 136, 316, 1672, 2007
BmiI GGNNCC 5 cut(s) 82, 171, 220, 1673, 1674
BmrFI CCNGG 6 cut(s) 174, 768, 1242, 1774, 1775, 2188
BmrI ACTGGG 1 cut(s) 572
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 3 cut(s) 88, 266, 769
BpmI CTGGAG 5 cut(s) 568, 706, 1071, 1305, 1452
Bpu10I CCTNAGC 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 329
BpuMI CCSGG 3 cut(s) 174, 1774, 1775
BsaAI YACGTR 2 cut(s) 1860, 2171
BsaBI GATNNNNATC 1 cut(s) 2236
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 2 cut(s) 2271, 2318
BsaJI CCNNGG 8 cut(s) 291, 396, 767, 1773, 1774, 2022, 2187, 2318
BsaXI ACNNNNNCTCC 2 cut(s) 879, 909
Bsc4I CCNNNNNNNGG 5 cut(s) 941, 1331, 1468, 1743, 2028
Bse118I RCCGGY 4 cut(s) 37, 77, 110, 340
Bse1I ACTGG 4 cut(s) 578, 829, 905, 1174
Bse21I CCTNAGG 2 cut(s) 502, 1326
Bse8I GATNNNNATC 1 cut(s) 2236
BseBI CCWGG 3 cut(s) 768, 1242, 2188
BseDI CCNNGG 8 cut(s) 291, 396, 767, 1773, 1774, 2022, 2187, 2318
BseJI GATNNNNATC 1 cut(s) 2236
BseLI CCNNNNNNNGG 5 cut(s) 941, 1331, 1468, 1743, 2028
BseMII CTCAG 7 cut(s) 42, 202, 426, 516, 1200, 1317, 1812
BseNI ACTGG 4 cut(s) 578, 829, 905, 1174
BseRI GAGGAG 1 cut(s) 56
BseX3I CGGCCG 1 cut(s) 342
BseXI GCAGC 6 cut(s) 640, 916, 1122, 1594, 1597, 1658
BseYI CCCAGC 1 cut(s) 1742
BsgI GTGCAG 2 cut(s) 58, 1023
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 8 cut(s) 41, 138, 206, 318, 344, 584, 2021, 2317
BshNI GGYRCC 2 cut(s) 80, 218
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 1307
BsiHKCI CYCGRG 1 cut(s) 1773
BsiSI CCGG 7 cut(s) 38, 78, 111, 174, 302, 341, 1774
BslFI GGGAC 4 cut(s) 248, 483, 1685, 2046
BslI CCNNNNNNNGG 5 cut(s) 941, 1331, 1468, 1743, 2028
BsmAI GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2271, 2318
BsmBI CGTCTC 2 cut(s) 31, 94
BsmFI GGGAC 4 cut(s) 248, 483, 1685, 2046
BsmI GAATGC 1 cut(s) 1552
BsnI GGCC 8 cut(s) 41, 138, 206, 318, 344, 584, 2021, 2317
Bso31I GGTCTC 2 cut(s) 2271, 2318
BsoBI CYCGRG 1 cut(s) 1773
Bsp119I TTCGAA 1 cut(s) 329
Bsp1286I GDGCHC 3 cut(s) 556, 1307, 1928
Bsp143I GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2128, 2149, 2197
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 7 cut(s) 12, 156, 243, 246, 532, 2084, 2258
BspANI GGCC 8 cut(s) 41, 138, 206, 318, 344, 584, 2021, 2317
BspCNI CTCAG 7 cut(s) 41, 201, 427, 515, 1201, 1318, 1813
BspHI TCATGA 1 cut(s) 2131
BspLI GGNNCC 5 cut(s) 82, 171, 220, 1673, 1674
BspMAI CTGCAG 1 cut(s) 658
BspMI ACCTGC 1 cut(s) 647
BspPI GGATC 8 cut(s) 748, 779, 952, 971, 1073, 1979, 2144, 2205
BspT104I TTCGAA 1 cut(s) 329
BspT107I GGYRCC 2 cut(s) 80, 218
BspTI CTTAAG 1 cut(s) 1355
BspTNI GGTCTC 2 cut(s) 2271, 2318
BsrFI RCCGGY 4 cut(s) 37, 77, 110, 340
BsrI ACTGG 4 cut(s) 578, 829, 905, 1174
BssAI RCCGGY 4 cut(s) 37, 77, 110, 340
BssECI CCNNGG 8 cut(s) 291, 396, 767, 1773, 1774, 2022, 2187, 2318
BssMI GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2128, 2149, 2197
BssNI GRCGYC 1 cut(s) 54
BssSI CACGAG 1 cut(s) 893
BssT1I CCWWGG 4 cut(s) 291, 396, 2022, 2318
Bst2BI CACGAG 1 cut(s) 893
Bst2UI CCWGG 3 cut(s) 768, 1242, 2188
Bst4CI ACNGT 8 cut(s) 85, 126, 223, 1199, 1309, 1657, 1791, 2419
Bst6I CTCTTC 1 cut(s) 2267
BstACI GRCGYC 1 cut(s) 54
BstAFI CTTAAG 1 cut(s) 1355
BstAPI GCANNNNNTGC 2 cut(s) 1619, 1892
BstBAI YACGTR 2 cut(s) 1860, 2171
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 342, 2112
BstDEI CTNAG 8 cut(s) 28, 188, 435, 502, 1209, 1326, 1821, 2177
BstDSI CCRYGG 1 cut(s) 291
BstEII GGTNACC 2 cut(s) 151, 698
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 2 cut(s) 63, 2224
BstKTI GATC 9 cut(s) 743, 774, 947, 979, 1068, 1987, 2131, 2152, 2200
BstMAI GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2271, 2318
BstMBI GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2128, 2149, 2197
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 9 cut(s) 18, 38, 398, 1030, 1110, 1619, 1646, 1892, 2300
BstNI CCWGG 3 cut(s) 768, 1242, 2188
BstNSI RCATGY 3 cut(s) 1399, 1690, 1810
BstPI GGTNACC 2 cut(s) 151, 698
BstSCI CCNGG 6 cut(s) 172, 766, 1240, 1772, 1773, 2186
BstSFI CTRYAG 3 cut(s) 654, 1547, 1575
BstV1I GCAGC 6 cut(s) 640, 916, 1122, 1594, 1597, 1658
BstV2I GAAGAC 3 cut(s) 88, 266, 769
BstX2I RGATCY 3 cut(s) 944, 976, 1984
BstYI RGATCY 3 cut(s) 944, 976, 1984
BstZI CGGCCG 1 cut(s) 342
Bsu36I CCTNAGG 2 cut(s) 502, 1326
BsuRI GGCC 8 cut(s) 41, 138, 206, 318, 344, 584, 2021, 2317
BtgI CCRYGG 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 1260
BtsI GCAGTG 2 cut(s) 1522, 2393
BtsIMutI CAGTG 5 cut(s) 585, 1131, 1314, 1522, 2393
BveI ACCTGC 1 cut(s) 647
Cac8I GCNNGC 2 cut(s) 342, 2112
CaiI CAGNNNCTG 4 cut(s) 124, 195, 1610, 1988
CciI TCATGA 1 cut(s) 2131
CfoI GCGC 2 cut(s) 63, 2224
Cfr10I RCCGGY 4 cut(s) 37, 77, 110, 340
Cfr13I GGNCC 4 cut(s) 136, 316, 1672, 2007
Cfr9I CCCGGG 1 cut(s) 1773
CseI GACGC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 1155, 2137
CspCI CAANNNNNGTGG 2 cut(s) 2088, 2123
CviAII CATG 6 cut(s) 292, 1396, 1571, 1687, 1807, 2132
CviQI GTAC 2 cut(s) 1155, 2137
DdeI CTNAG 8 cut(s) 28, 188, 435, 502, 1209, 1326, 1821, 2177
DpnI GATC 9 cut(s) 742, 773, 946, 978, 1067, 1986, 2130, 2151, 2199
DpnII GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2128, 2149, 2197
DrdI GACNNNNNNGTC 1 cut(s) 1273
DseDI GACNNNNNNGTC 1 cut(s) 1273
EaeI YGGCCR 2 cut(s) 342, 582
EagI CGGCCG 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 2267
EarI CTCTTC 1 cut(s) 2267
EclXI CGGCCG 1 cut(s) 342
Eco130I CCWWGG 4 cut(s) 291, 396, 2022, 2318
Eco147I AGGCCT 1 cut(s) 2317
Eco24I GRGCYC 2 cut(s) 556, 1928
Eco31I GGTCTC 2 cut(s) 2271, 2318
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 1672, 2007
Eco52I CGGCCG 1 cut(s) 342
Eco57I CTGAAG 1 cut(s) 82
Eco72I CACGTG 1 cut(s) 2171
Eco81I CCTNAGG 2 cut(s) 502, 1326
Eco88I CYCGRG 1 cut(s) 1773
Eco91I GGTNACC 2 cut(s) 151, 698
EcoO109I RGGNCCY 1 cut(s) 1672
EcoO65I GGTNACC 2 cut(s) 151, 698
EcoRI GAATTC 2 cut(s) 675, 1747
EcoRII CCWGG 3 cut(s) 766, 1240, 2186
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 4 cut(s) 291, 396, 2022, 2318
EcoT22I ATGCAT 1 cut(s) 2239
EcoT38I GRGCYC 2 cut(s) 556, 1928
ErhI CCWWGG 4 cut(s) 291, 396, 2022, 2318
Esp3I CGTCTC 2 cut(s) 31, 94
FaeI CATG 6 cut(s) 295, 1399, 1574, 1690, 1810, 2135
FalI AAGNNNNNCTT 6 cut(s) 1106, 1138, 1305, 1337, 2094, 2126
FaqI GGGAC 4 cut(s) 248, 483, 1685, 2046
FatI CATG 6 cut(s) 291, 1395, 1570, 1686, 1806, 2131
FauNDI CATATG 4 cut(s) 1106, 1486, 2155, 2299
Fnu4HI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
FriOI GRGCYC 2 cut(s) 556, 1928
Fsp4HI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
FspBI CTAG 6 cut(s) 471, 647, 728, 908, 974, 2399
GlaI GCGC 2 cut(s) 62, 2223
GluI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
GsaI CCCAGC 1 cut(s) 1746
GsuI CTGGAG 5 cut(s) 568, 706, 1071, 1305, 1452
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 8 cut(s) 41, 138, 206, 318, 344, 584, 2021, 2317
HapII CCGG 7 cut(s) 38, 78, 111, 174, 302, 341, 1774
HgaI GACGC 1 cut(s) 62
HhaI GCGC 2 cut(s) 63, 2224
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 6 cut(s) 295, 1399, 1574, 1690, 1810, 2135
Hin6I GCGC 2 cut(s) 61, 2222
HinP1I GCGC 2 cut(s) 61, 2222
HincII GTYRAC 1 cut(s) 2212
HindII GTYRAC 1 cut(s) 2212
HindIII AAGCTT 2 cut(s) 536, 1838
HpaII CCGG 7 cut(s) 38, 78, 111, 174, 302, 341, 1774
HphI GGTGA 6 cut(s) 145, 346, 671, 731, 2049, 2088
Hpy166II GTNNAC 5 cut(s) 898, 1401, 1681, 2174, 2212
Hpy188I TCNGA 6 cut(s) 715, 853, 1210, 1372, 1753, 1822
Hpy8I GTNNAC 5 cut(s) 898, 1401, 1681, 2174, 2212
Hpy99I CGWCG 2 cut(s) 56, 89
HpyCH4III ACNGT 8 cut(s) 85, 126, 223, 1199, 1309, 1657, 1791, 2419
HpyCH4IV ACGT 4 cut(s) 24, 1092, 1859, 2170
HpyF10VI GCNNNNNNNGC 9 cut(s) 18, 38, 398, 1030, 1110, 1619, 1646, 1892, 2300
HpyF3I CTNAG 8 cut(s) 28, 188, 435, 502, 1209, 1326, 1821, 2177
HpySE526I ACGT 4 cut(s) 24, 1092, 1859, 2170
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 6 cut(s) 295, 1399, 1574, 1690, 1810, 2135
HspAI GCGC 2 cut(s) 61, 2222
KflI GGGWCCC 1 cut(s) 1672
KroI GCCGGC 1 cut(s) 340
KroNI GCCGGC 1 cut(s) 342
Kzo9I GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2128, 2149, 2197
LmnI GCTCC 5 cut(s) 857, 1077, 1803, 2251, 2308
Lsp1109I GCAGC 6 cut(s) 640, 916, 1122, 1594, 1597, 1658
MaeI CTAG 6 cut(s) 471, 647, 728, 908, 974, 2399
MaeII ACGT 4 cut(s) 24, 1092, 1859, 2170
MaeIII GTNAC 7 cut(s) 151, 176, 698, 719, 1264, 1517, 1855
MalI GATC 9 cut(s) 742, 773, 946, 978, 1067, 1986, 2130, 2151, 2199
MboI GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2128, 2149, 2197
MboII GAAGA 9 cut(s) 88, 271, 774, 840, 1074, 1313, 1862, 2284, 2345
MflI RGATCY 3 cut(s) 944, 976, 1984
MhlI GDGCHC 3 cut(s) 556, 1307, 1928
MlsI TGGCCA 1 cut(s) 584
MluNI TGGCCA 1 cut(s) 584
MlyI GAGTC 3 cut(s) 446, 788, 1832
MmeI TCCRAC 3 cut(s) 72, 111, 659
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 1 cut(s) 2239
MroNI GCCGGC 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 888
MscI TGGCCA 1 cut(s) 584
MseI TTAA 5 cut(s) 831, 962, 1317, 1356, 2046
MslI CAYNNNNRTG 3 cut(s) 1406, 1425, 1691
Msp20I TGGCCA 1 cut(s) 584
MspA1I CMGCKG 2 cut(s) 1607, 1649
MspCI CTTAAG 1 cut(s) 1355
MspI CCGG 7 cut(s) 38, 78, 111, 174, 302, 341, 1774
MspR9I CCNGG 6 cut(s) 174, 768, 1242, 1774, 1775, 2188
Mva1269I GAATGC 1 cut(s) 1552
MvaI CCWGG 3 cut(s) 768, 1242, 2188
MwoI GCNNNNNNNGC 9 cut(s) 18, 38, 398, 1030, 1110, 1619, 1646, 1892, 2300
NaeI GCCGGC 1 cut(s) 342
NciI CCSGG 3 cut(s) 174, 1774, 1775
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 4 cut(s) 1106, 1486, 2155, 2299
NdeII GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2128, 2149, 2197
NgoMIV GCCGGC 1 cut(s) 340
NlaIII CATG 6 cut(s) 295, 1399, 1574, 1690, 1810, 2135
NlaIV GGNNCC 5 cut(s) 82, 171, 220, 1673, 1674
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 4 cut(s) 151, 719, 1264, 1517
NsiI ATGCAT 1 cut(s) 2239
NspI RCATGY 3 cut(s) 1399, 1690, 1810
NspV TTCGAA 1 cut(s) 329
OliI CACNNNNGTG 1 cut(s) 1406
PagI TCATGA 1 cut(s) 2131
PaqCI CACCTGC 1 cut(s) 647
PceI AGGCCT 1 cut(s) 2317
PciI ACATGT 1 cut(s) 1395
PctI GAATGC 1 cut(s) 1552
PdiI GCCGGC 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 888
PfeI GAWTC 8 cut(s) 326, 415, 566, 968, 1373, 1525, 1871, 2390
PflFI GACNNNGTC 1 cut(s) 436
PflMI CCANNNNNTGG 1 cut(s) 941
PkrI GCNGC 9 cut(s) 14, 244, 247, 655, 906, 1112, 1609, 1612, 1648
PleI GAGTC 3 cut(s) 445, 788, 1831
PmaCI CACGTG 1 cut(s) 2171
PmlI CACGTG 1 cut(s) 2171
PpsI GAGTC 3 cut(s) 445, 788, 1831
Ppu21I YACGTR 2 cut(s) 1860, 2171
PpuMI RGGWCCY 1 cut(s) 1672
PscI ACATGT 1 cut(s) 1395
PshBI ATTAAT 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 1672
Psp6I CCWGG 3 cut(s) 766, 1240, 2186
PspCI CACGTG 1 cut(s) 2171
PspEI GGTNACC 2 cut(s) 151, 698
PspFI CCCAGC 1 cut(s) 1742
PspGI CCWGG 3 cut(s) 766, 1240, 2186
PspN4I GGNNCC 5 cut(s) 82, 171, 220, 1673, 1674
PspPI GGNCC 4 cut(s) 136, 316, 1672, 2007
PspPPI RGGWCCY 1 cut(s) 1672
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 4 cut(s) 124, 195, 1610, 1988
PsuI RGATCY 3 cut(s) 944, 976, 1984
PsyI GACNNNGTC 1 cut(s) 436
PvuII CAGCTG 2 cut(s) 1607, 1649
RsaI GTAC 2 cut(s) 1156, 2138
RsaNI GTAC 2 cut(s) 1155, 2137
RseI CAYNNNNRTG 3 cut(s) 1406, 1425, 1691
SaqAI TTAA 5 cut(s) 831, 962, 1317, 1356, 2046
SatI GCNGC 9 cut(s) 13, 243, 246, 654, 905, 1111, 1608, 1611, 1647
Sau3AI GATC 9 cut(s) 740, 771, 944, 976, 1065, 1984, 2128, 2149, 2197
Sau96I GGNCC 4 cut(s) 136, 316, 1672, 2007
SchI GAGTC 3 cut(s) 446, 788, 1832
ScrFI CCNGG 6 cut(s) 174, 768, 1242, 1774, 1775, 2188
SduI GDGCHC 3 cut(s) 556, 1307, 1928
SfcI CTRYAG 3 cut(s) 654, 1547, 1575
SfuI TTCGAA 1 cut(s) 329
SinI GGWCC 2 cut(s) 1672, 2007
SmaI CCCGGG 1 cut(s) 1775
SmiMI CAYNNNNRTG 3 cut(s) 1406, 1425, 1691
SmlI CTYRAG 1 cut(s) 1355
SmoI CTYRAG 1 cut(s) 1355
SpeI ACTAGT 1 cut(s) 727
SseBI AGGCCT 1 cut(s) 2317
SsiI CCGC 7 cut(s) 12, 156, 243, 246, 532, 2084, 2258
SspMI CTAG 6 cut(s) 471, 647, 728, 908, 974, 2399
StuI AGGCCT 1 cut(s) 2317
StyD4I CCNGG 6 cut(s) 172, 766, 1240, 1772, 1773, 2186
StyI CCWWGG 4 cut(s) 291, 396, 2022, 2318
TaaI ACNGT 8 cut(s) 85, 126, 223, 1199, 1309, 1657, 1791, 2419
TaiI ACGT 4 cut(s) 27, 1095, 1862, 2173
TaqI TCGA 3 cut(s) 329, 442, 1125
TatI WGTACW 1 cut(s) 2136
TauI GCSGC 3 cut(s) 15, 245, 248
TfiI GAWTC 8 cut(s) 326, 415, 566, 968, 1373, 1525, 1871, 2390
Tru1I TTAA 5 cut(s) 831, 962, 1317, 1356, 2046
Tru9I TTAA 5 cut(s) 831, 962, 1317, 1356, 2046
TscAI CASTG 5 cut(s) 585, 1138, 1314, 1522, 2400
TseFI GTSAC 4 cut(s) 151, 719, 1264, 1517
TseI GCWGC 6 cut(s) 653, 904, 1110, 1607, 1610, 1646
Tsp45I GTSAC 4 cut(s) 151, 719, 1264, 1517
TspGWI ACGGA 3 cut(s) 82, 338, 1999
TspMI CCCGGG 1 cut(s) 1773
TspRI CASTG 5 cut(s) 585, 1138, 1314, 1522, 2400
Tth111I GACNNNGTC 1 cut(s) 436
Van91I CCANNNNNTGG 1 cut(s) 941
Vha464I CTTAAG 1 cut(s) 1355
VpaK11BI GGWCC 2 cut(s) 1672, 2007
VspI ATTAAT 1 cut(s) 831
XapI RAATTY 9 cut(s) 509, 610, 675, 707, 926, 1014, 1203, 1387, 1747
XbaI TCTAGA 1 cut(s) 973
XceI RCATGY 3 cut(s) 1399, 1690, 1810
XcmI CCANNNNNNNNNTGG 1 cut(s) 2001
XmaI CCCGGG 1 cut(s) 1773
XmnI GAANNNNTTC 1 cut(s) 888
XspI CTAG 6 cut(s) 471, 647, 728, 908, 974, 2399
Zsp2I ATGCAT 1 cut(s) 2239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.