Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007925

Physical Location & Seq

Genomic Coordinates Forward (+)

33752390 .. 33755470

Gene Structure 3081 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1263500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGCCTCCTTCCACCCCGTCTAGAATACTCATAGGCATCTCTTCCGATGCAGATGACAGTAAGGAGTTGCTCTCAAGGGCAATCAGAGTTCTTGCTCATCAAAATGACAACATCGTTGCCATCCATGTTCTTGTTGGCGAGGAGAGGAAAAAGCACGAATCAGGAACAAAAAATCAATCGCAGCTTCGTCGAGCAAAAGCCAATGTTATATCTGTGCTTGGAGAATTTGCCAGGAGTTGCCAATTTAAGCAGGTAAATTTAGAAGCAAGAGTAGTTTTTAGCTCAAGTGTTGGAAGAGGTCTGATTGAGGAAGCAAAATCAATTTCAGCAGACTATCTTCTTCTTGGTGGCTCAAGAAACCAATCCAAAAGAACTCCATCCATCACAAAATATTGCTTCAAGCATTCCCCGGCTAGTTGCACACTGGTTTTACTTGGGAAATCAAAGCAACCTCATCAGATTTCAAATTCAGAATCTGCTGACTCCAAAGAAATCCTTCAATCAAGTTCACGGACATCGTTGCAAGATATCATGCACAGTAGCAGAACTACATCCTTTTCTGAAAAGCAAACTGTCCAAGAAACACGGAACAAAAACCCTTCACCAAGAACAGTTTTATGTGAATTTGAAGCTGAATCTCAGAGCACAGAAGATGATACCAGTAGCAATGCAGACTCGACCGTTACAGCGTCCCCCTCTCTTGCTTCTCCCAATTTCAATAGCCAAATCACCCTAAGAAAGCAAGGATTGTCACCTTGCAGGATCATTACTTCGTTTTTAGGTTCACCATTCAGAAAACGAAACGGAAGTTTATCCAAGAACGAACTGCAGCTACCTTTGTTGAAGTGCTTCAGCTACAAGGAGATCGTGAATTCCACAAACAACTTCCACCCAGATAATATAGTAGGTCGAGGTGGATATTCAGAAGTATATAAGGGTGATCTTTCTGATGGAAGACGCATTGCAGTTAAGATGTTGGCCAAGGACAGCAAGGATGCTAACAAGGAAAAAGAGTTCCTCACGGAATTGGGTGTAATTTTACATGTCAGCCATCCTAATACCGCTAACTTAGTCGGTTGCTGCATTGAAAACGGACTCTACCTGATTTTCGATTTCTCTCCAAAAGGAAATTTGTCTTCTGCTTTGCACGGTAAATCCAGCGAGTTGCTTTCATGGCCTATTAGATACAAGATTGCCATTGGAGTTGCAAGGGGTCTGCACTATCTACATGAATGTTGCAAGCACCGAATAATCCATCGGGATATAAAAGCCTCAAATATCCTTCTGGGAACAGATTATGAACCGCAGATTACAGACTTTGGACTAGCAAAGTGGCTTCCTAACAAATGGACTCATCATGCTGTGCCTCCAATTGAGGGTACGTTTGGATACTTAGCACCGGAGTACTTCATGAACGGAATTGTGGATGAAAAAATTGATGTTTTTGCATTTGGAGTTCTTCTTATGGAAATTGTAACTGGAAGAAAGCCTGTGGATTCATCAAAGCAAAACCTTCTACTATGGGCAAAGCCTCTTATGGAATCAGGGGACATAGCCAAACTAGCTGATCCAAAACTGGAAGGGAAATATGACACAGAACAACTGCACAGAATAGTGCTTACAGCTTCCGATTGTGTAAGACAATCCTCAGTATGGCGACCCCCAATGCGTGAGGTGTTAGAGCTTCTTACCAATGGCCATAACTCTAAGGTTGCAAGGAGCTCGATTCCTAAGTTTTCCTCGGACGAGTTGGATGATTGCTCCACGGTTTATGGATATGAGGTTCCAGTAGATATTGATTTGGAGGACTACTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Usp

Protein Analysis

606

Amino Acids

67.14

Weight (kDa)

8.3

Isoelectric Point (pI)

51.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Usp	PF00582	8 - 143	4.3e-07	Universal stress protein family
Pkinase	PF00069	297 - 506	2e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	300 - 505	1.1e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007925)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57670
fragaria_vesca	FvH4_4g08510 FvH4_4g08510 FvH4_4g08510 FvH4_4g08510
malus_domestica	MD13G1263500.v1.1 MD16G1264900.v1.1
prunus_persica	Prupe.1G094200_v2.0.a1 Prupe.1G094200_v2.0.a1 Prupe.1G094200_v2.0.a1 Prupe.1G094200_v2.0.a1
pyrus_communis	pycom16g23440
rosa_chinensis	RchiOBHm_Chr4g0403851 RchiOBHm_Chr4g0403861
rosa_laevigata	RLG00000008945
rosa_multiflora	Rmu_sc0005134.1_g000014
rosa_roxburghii	Rroxscaffold_5G00348470
rosa_rugosa	Rorug04G0046100 Rorug04G0046100
rosa_samantha	Rh4AG121300 Rh4BG114600 Rh4CG129900 Rh4DG114600
rosa_wichuraiana	Rw4G009890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	241
AciI	CCGC	2 cut(s)	1062, 1304
AclWI	GGATC	2 cut(s)	770, 1562
AcoI	YGGCCR	2 cut(s)	978, 1696
AcsI	RAATTY	6 cut(s)	224, 256, 466, 623, 871, 1129
AcuI	CTGAAG	1 cut(s)	835
AfaI	GTAC	2 cut(s)	1381, 1406
AfiI	CCNNNNNNNGG	2 cut(s)	1376, 1653
AflIII	ACRYGT	1 cut(s)	1042
AgsI	TTSAA	7 cut(s)	400, 465, 500, 629, 718, 844, 1088
AjnI	CCWGG	1 cut(s)	230
AloI	GAACNNNNNNTCC	4 cut(s)	998, 1030, 1767, 1799
AluBI	AGCT	9 cut(s)	184, 282, 632, 832, 855, 1565, 1625, 1684, 1722
AluI	AGCT	9 cut(s)	184, 282, 632, 832, 855, 1565, 1625, 1684, 1722
Alw21I	GWGCWC	2 cut(s)	647, 1724
AlwI	GGATC	2 cut(s)	770, 1562
AlwNI	CAGNNNCTG	1 cut(s)	476
AoxI	GGCC	3 cut(s)	978, 1175, 1696
ApeKI	GCWGC	3 cut(s)	181, 829, 1080
ApoI	RAATTY	6 cut(s)	224, 256, 466, 623, 871, 1129
Asp700I	GAANNNNTTC	2 cut(s)	495, 848
AsuC2I	CCSGG	1 cut(s)	410
AsuHPI	GGTGA	5 cut(s)	594, 721, 744, 777, 950
BalI	TGGCCA	2 cut(s)	980, 1698
BanII	GRGCYC	1 cut(s)	1724
BbsI	GAAGAC	2 cut(s)	961, 1128
Bbv12I	GWGCWC	2 cut(s)	647, 1724
BbvI	GCAGC	3 cut(s)	193, 841, 1067
BccI	CCATC	6 cut(s)	128, 385, 389, 944, 1059, 1263
BcgI	CGANNNNNNTGC	4 cut(s)	170, 204, 1704, 1738
BciT130I	CCWGG	1 cut(s)	232
BciVI	GTATCC	1 cut(s)	1382
BcnI	CCSGG	1 cut(s)	410
BfaI	CTAG	4 cut(s)	21, 414, 1325, 1562
BfmI	CTRYAG	1 cut(s)	827
BfuAI	ACCTGC	1 cut(s)	241
BfuI	GTATCC	1 cut(s)	1382
BisI	GCNGC	3 cut(s)	182, 830, 1081
BlsI	GCNGC	3 cut(s)	183, 831, 1082
BmcAI	AGTACT	1 cut(s)	1406
Bme1390I	CCNGG	2 cut(s)	232, 410
BmiI	GGNNCC	1 cut(s)	1785
BmrFI	CCNGG	2 cut(s)	232, 410
BmsI	GCATC	3 cut(s)	37, 45, 985
BpiI	GAAGAC	2 cut(s)	961, 1128
BplI	GAGNNNNNCTC	2 cut(s)	56, 88
BpuEI	CTTGAG	3 cut(s)	58, 268, 337
BpuMI	CCSGG	1 cut(s)	410
BsaJI	CCNNGG	4 cut(s)	408, 981, 1740, 1764
BsaWI	WCCGGW	1 cut(s)	1399
Bsc4I	CCNNNNNNNGG	2 cut(s)	1376, 1653
Bse1I	ACTGG	5 cut(s)	429, 660, 1483, 1581, 1787
Bse3DI	GCAATG	2 cut(s)	673, 960
BseBI	CCWGG	1 cut(s)	232
BseDI	CCNNGG	4 cut(s)	408, 981, 1740, 1764
BseGI	GGATG	7 cut(s)	120, 377, 551, 1000, 1051, 1432, 1759
BseLI	CCNNNNNNNGG	2 cut(s)	1376, 1653
BseMI	GCAATG	2 cut(s)	673, 960
BseMII	CTCAG	2 cut(s)	653, 1662
BseNI	ACTGG	5 cut(s)	429, 660, 1483, 1581, 1787
BseRI	GAGGAG	1 cut(s)	155
BseXI	GCAGC	3 cut(s)	193, 841, 1067
BsgI	GTGCAG	2 cut(s)	1202, 1589
Bsh1285I	CGRYCG	1 cut(s)	681
BshFI	GGCC	3 cut(s)	980, 1177, 1698
BsiEI	CGRYCG	1 cut(s)	681
BsiHKAI	GWGCWC	2 cut(s)	647, 1724
BsiSI	CCGG	2 cut(s)	410, 1400
BslFI	GGGAC	2 cut(s)	676, 1562
BslI	CCNNNNNNNGG	2 cut(s)	1376, 1653
BsmFI	GGGAC	2 cut(s)	676, 1562
BsmI	GAATGC	1 cut(s)	403
BsnI	GGCC	3 cut(s)	980, 1177, 1698
Bsp1286I	GDGCHC	2 cut(s)	647, 1724
Bsp143I	GATC	4 cut(s)	762, 864, 940, 1567
BspACI	CCGC	2 cut(s)	1062, 1304
BspANI	GGCC	3 cut(s)	980, 1177, 1698
BspCNI	CTCAG	2 cut(s)	652, 1661
BspHI	TCATGA	1 cut(s)	1410
BspLI	GGNNCC	1 cut(s)	1785
BspMAI	CTGCAG	1 cut(s)	831
BspMI	ACCTGC	1 cut(s)	241
BspPI	GGATC	2 cut(s)	770, 1562
BsrDI	GCAATG	2 cut(s)	673, 960
BsrI	ACTGG	5 cut(s)	429, 660, 1483, 1581, 1787
BssECI	CCNNGG	4 cut(s)	408, 981, 1740, 1764
BssMI	GATC	4 cut(s)	762, 864, 940, 1567
BssT1I	CCWWGG	1 cut(s)	981
Bst2UI	CCWGG	1 cut(s)	232
Bst4CI	ACNGT	7 cut(s)	59, 539, 574, 613, 682, 1151, 1768
Bst6I	CTCTTC	2 cut(s)	46, 289
BstC8I	GCNNGC	1 cut(s)	1241
BstDEI	CTNAG	7 cut(s)	639, 734, 1069, 1393, 1648, 1707, 1731
BstDSI	CCRYGG	1 cut(s)	1764
BstF5I	GGATG	7 cut(s)	120, 377, 551, 1000, 1051, 1432, 1759
BstKTI	GATC	4 cut(s)	765, 867, 943, 1570
BstMBI	GATC	4 cut(s)	762, 864, 940, 1567
BstMCI	CGRYCG	1 cut(s)	681
BstMWI	GCNNNNNNNGC	2 cut(s)	1174, 1562
BstNI	CCWGG	1 cut(s)	232
BstNSI	RCATGY	1 cut(s)	1046
BstSCI	CCNGG	2 cut(s)	230, 408
BstSFI	CTRYAG	1 cut(s)	827
BstV1I	GCAGC	3 cut(s)	193, 841, 1067
BstV2I	GAAGAC	2 cut(s)	961, 1128
BsuI	GTATCC	1 cut(s)	1382
BsuRI	GGCC	3 cut(s)	980, 1177, 1698
BtgI	CCRYGG	1 cut(s)	1764
BtsCI	GGATG	7 cut(s)	120, 377, 551, 1000, 1051, 1432, 1759
BtsIMutI	CAGTG	1 cut(s)	422
BveI	ACCTGC	1 cut(s)	241
Cac8I	GCNNGC	1 cut(s)	1241
CaiI	CAGNNNCTG	1 cut(s)	476
CciI	TCATGA	1 cut(s)	1410
CseI	GACGC	2 cut(s)	678, 966
Csp6I	GTAC	2 cut(s)	1380, 1405
CviAII	CATG	7 cut(s)	125, 532, 1043, 1173, 1229, 1358, 1411
CviQI	GTAC	2 cut(s)	1380, 1405
DdeI	CTNAG	7 cut(s)	639, 734, 1069, 1393, 1648, 1707, 1731
DpnI	GATC	4 cut(s)	764, 866, 942, 1569
DpnII	GATC	4 cut(s)	762, 864, 940, 1567
EaeI	YGGCCR	2 cut(s)	978, 1696
Eam1104I	CTCTTC	2 cut(s)	46, 289
EarI	CTCTTC	2 cut(s)	46, 289
Ecl136II	GAGCTC	1 cut(s)	1722
Eco130I	CCWWGG	1 cut(s)	981
Eco24I	GRGCYC	1 cut(s)	1724
Eco32I	GATATC	1 cut(s)	529
Eco53kI	GAGCTC	1 cut(s)	1722
Eco57I	CTGAAG	1 cut(s)	835
EcoICRI	GAGCTC	1 cut(s)	1722
EcoRI	GAATTC	1 cut(s)	871
EcoRII	CCWGG	1 cut(s)	230
EcoRV	GATATC	1 cut(s)	529
EcoT14I	CCWWGG	1 cut(s)	981
EcoT38I	GRGCYC	1 cut(s)	1724
ErhI	CCWWGG	1 cut(s)	981
FaeI	CATG	7 cut(s)	128, 535, 1046, 1176, 1232, 1361, 1414
FalI	AAGNNNNNCTT	2 cut(s)	480, 512
FaqI	GGGAC	2 cut(s)	676, 1562
FatI	CATG	7 cut(s)	124, 531, 1042, 1172, 1228, 1357, 1410
Fnu4HI	GCNGC	3 cut(s)	182, 830, 1081
FokI	GGATG	7 cut(s)	107, 364, 538, 1007, 1038, 1439, 1766
FriOI	GRGCYC	1 cut(s)	1724
Fsp4HI	GCNGC	3 cut(s)	182, 830, 1081
FspBI	CTAG	4 cut(s)	21, 414, 1325, 1562
GluI	GCNGC	3 cut(s)	182, 830, 1081
HaeIII	GGCC	3 cut(s)	980, 1177, 1698
HapII	CCGG	2 cut(s)	410, 1400
HgaI	GACGC	2 cut(s)	678, 966
Hin1II	CATG	7 cut(s)	128, 535, 1046, 1176, 1232, 1361, 1414
HpaII	CCGG	2 cut(s)	410, 1400
HphI	GGTGA	5 cut(s)	594, 721, 744, 777, 950
Hpy166II	GTNNAC	2 cut(s)	509, 785
Hpy188III	TCNNGA	6 cut(s)	21, 162, 354, 868, 1259, 1411
Hpy8I	GTNNAC	2 cut(s)	509, 785
Hpy99I	CGWCG	1 cut(s)	192
HpyAV	CCTTC	6 cut(s)	18, 506, 609, 1292, 1523, 1574
HpyCH4III	ACNGT	7 cut(s)	59, 539, 574, 613, 682, 1151, 1768
HpyCH4IV	ACGT	1 cut(s)	1382
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1174, 1562
HpyF3I	CTNAG	7 cut(s)	639, 734, 1069, 1393, 1648, 1707, 1731
HpySE526I	ACGT	1 cut(s)	1382
Hsp92II	CATG	7 cut(s)	128, 535, 1046, 1176, 1232, 1361, 1414
Kzo9I	GATC	4 cut(s)	762, 864, 940, 1567
LmnI	GCTCC	2 cut(s)	1719, 1766
Lsp1109I	GCAGC	3 cut(s)	193, 841, 1067
LweI	GCATC	3 cut(s)	37, 45, 985
MaeI	CTAG	4 cut(s)	21, 414, 1325, 1562
MaeII	ACGT	1 cut(s)	1382
MaeIII	GTNAC	3 cut(s)	682, 750, 1474
MalI	GATC	4 cut(s)	764, 866, 942, 1569
MboI	GATC	4 cut(s)	762, 864, 940, 1567
MboII	GAAGA	9 cut(s)	33, 306, 329, 332, 662, 966, 1128, 1451, 1494
MfeI	CAATTG	1 cut(s)	1371
MhlI	GDGCHC	2 cut(s)	647, 1724
MlsI	TGGCCA	2 cut(s)	980, 1698
MluNI	TGGCCA	2 cut(s)	980, 1698
MlyI	GAGTC	4 cut(s)	476, 668, 1089, 1345
MmeI	TCCRAC	2 cut(s)	271, 1731
Mox20I	TGGCCA	2 cut(s)	980, 1698
MroXI	GAANNNNTTC	2 cut(s)	495, 848
MscI	TGGCCA	2 cut(s)	980, 1698
MseI	TTAA	2 cut(s)	246, 969
MslI	CAYNNNNRTG	1 cut(s)	102
Msp20I	TGGCCA	2 cut(s)	980, 1698
MspI	CCGG	2 cut(s)	410, 1400
MspR9I	CCNGG	2 cut(s)	232, 410
MunI	CAATTG	1 cut(s)	1371
Mva1269I	GAATGC	1 cut(s)	403
MvaI	CCWGG	1 cut(s)	232
MwoI	GCNNNNNNNGC	2 cut(s)	1174, 1562
NciI	CCSGG	1 cut(s)	410
NdeII	GATC	4 cut(s)	762, 864, 940, 1567
NlaIII	CATG	7 cut(s)	128, 535, 1046, 1176, 1232, 1361, 1414
NlaIV	GGNNCC	1 cut(s)	1785
NmuCI	GTSAC	1 cut(s)	750
NspI	RCATGY	1 cut(s)	1046
PagI	TCATGA	1 cut(s)	1410
PciI	ACATGT	1 cut(s)	1042
PctI	GAATGC	1 cut(s)	403
PdmI	GAANNNNTTC	2 cut(s)	495, 848
PfeI	GAWTC	6 cut(s)	158, 473, 635, 1496, 1541, 1726
PkrI	GCNGC	3 cut(s)	183, 831, 1082
PleI	GAGTC	4 cut(s)	476, 668, 1089, 1345
PpsI	GAGTC	4 cut(s)	476, 668, 1089, 1345
PscI	ACATGT	1 cut(s)	1042
Psp124BI	GAGCTC	1 cut(s)	1724
Psp6I	CCWGG	1 cut(s)	230
PspGI	CCWGG	1 cut(s)	230
PspN4I	GGNNCC	1 cut(s)	1785
PsrI	GAACNNNNNNTAC	2 cut(s)	816, 848
PstI	CTGCAG	1 cut(s)	831
PstNI	CAGNNNCTG	1 cut(s)	476
RsaI	GTAC	2 cut(s)	1381, 1406
RsaNI	GTAC	2 cut(s)	1380, 1405
RseI	CAYNNNNRTG	1 cut(s)	102
SacI	GAGCTC	1 cut(s)	1724
SaqAI	TTAA	2 cut(s)	246, 969
SatI	GCNGC	3 cut(s)	182, 830, 1081
Sau3AI	GATC	4 cut(s)	762, 864, 940, 1567
ScaI	AGTACT	1 cut(s)	1406
SchI	GAGTC	4 cut(s)	476, 668, 1089, 1345
ScrFI	CCNGG	2 cut(s)	232, 410
SduI	GDGCHC	2 cut(s)	647, 1724
SfaNI	GCATC	3 cut(s)	37, 45, 985
SfcI	CTRYAG	1 cut(s)	827
SmiMI	CAYNNNNRTG	1 cut(s)	102
SmlI	CTYRAG	3 cut(s)	73, 283, 352
SmoI	CTYRAG	3 cut(s)	73, 283, 352
SsiI	CCGC	2 cut(s)	1062, 1304
SspI	AATATT	1 cut(s)	392
SspMI	CTAG	4 cut(s)	21, 414, 1325, 1562
SstI	GAGCTC	1 cut(s)	1724
StyD4I	CCNGG	2 cut(s)	230, 408
StyI	CCWWGG	1 cut(s)	981
TaaI	ACNGT	7 cut(s)	59, 539, 574, 613, 682, 1151, 1768
TaiI	ACGT	1 cut(s)	1385
TaqI	TCGA	5 cut(s)	190, 677, 910, 1110, 1724
TatI	WGTACW	1 cut(s)	1404
TfiI	GAWTC	6 cut(s)	158, 473, 635, 1496, 1541, 1726
Tru1I	TTAA	2 cut(s)	246, 969
Tru9I	TTAA	2 cut(s)	246, 969
TscAI	CASTG	1 cut(s)	429
TseFI	GTSAC	1 cut(s)	750
TseI	GCWGC	3 cut(s)	181, 829, 1080
Tsp45I	GTSAC	1 cut(s)	750
TspDTI	ATGAA	7 cut(s)	1161, 1245, 1314, 1399, 1427, 1443, 1488
TspGWI	ACGGA	6 cut(s)	526, 601, 819, 1037, 1107, 1431
TspRI	CASTG	1 cut(s)	429
XapI	RAATTY	6 cut(s)	224, 256, 466, 623, 871, 1129
XbaI	TCTAGA	1 cut(s)	20
XceI	RCATGY	1 cut(s)	1046
XcmI	CCANNNNNNNNNTGG	1 cut(s)	131
XmnI	GAANNNNTTC	2 cut(s)	495, 848
XspI	CTAG	4 cut(s)	21, 414, 1325, 1562
ZrmI	AGTACT	1 cut(s)	1406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.