MD14G1043200.v1.1

Mal d 1-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008603
Physical Location & Seq
Forward (+)
4077608 .. 4079569
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1043200.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGGGTGGGTGTGGAGGGACGACGAGGATGATGACAACAATTCAACGGCGATTGACATAAATCCTAGATCGGACGGCGATCGATGCTTGACGAGGAAGGTGGTGAAGATGCAGTGCAAGACTGAGGAGGTGGAGCCTGGAAAGTTCGTCAGGAAGTGCGAGAAGACCGAGCAGCTTCTCAGGGACTGCGCCGGCCGGCCTGTAGAAGTGGTGCAATCCAACAAAGAGTACACTGAACATGACGTCACTGACGAGGTGCTCAAAGGGTCTGCCACCTTTGGGTCATCACAGCACGGAGCATTGGACTTCCCAGGGCTACGCGGTGACATTGAAGACATTGAACGCAACTTTATGGGTGGCCTCAGCCGATTCTTTGAGGCTGCTGAGGAGTTGAAGAGCGGCTTCTTCAGTGCTTTTGGGAACATCTTTGATGAGGGACCTTCCTCCCCACTACCTCCTAGGAGGCGCGAGGTACCCATAGAAGGCCATCATCAGCAGGAAGCTTTTCCTAAAGCAAGCGGTAGCGACGACTCAGGACACGTTGATCTCTCTGGCTTGGCAAGAGAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

21.18

Weight (kDa)

4.78

Isoelectric Point (pI)

57.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 246
Acc65I GGTACC 1 cut(s) 472
AccB1I GGYRCC 1 cut(s) 472
AccBSI CCGCTC 1 cut(s) 399
AccII CGCG 2 cut(s) 321, 468
AciI CCGC 3 cut(s) 321, 399, 519
AcoI YGGCCR 1 cut(s) 193
AcuI CTGAAG 1 cut(s) 391
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 2 cut(s) 230, 474
AfiI CCNNNNNNNGG 2 cut(s) 279, 482
AflIII ACRYGT 1 cut(s) 538
AgsI TTSAA 4 cut(s) 45, 332, 341, 394
AjnI CCWGG 2 cut(s) 136, 310
AluBI AGCT 2 cut(s) 175, 503
AluI AGCT 2 cut(s) 175, 503
Alw21I GWGCWC 1 cut(s) 261
AlwNI CAGNNNCTG 1 cut(s) 186
AoxI GGCC 4 cut(s) 193, 197, 358, 484
ApeKI GCWGC 2 cut(s) 172, 380
Asp700I GAANNNNTTC 1 cut(s) 504
Asp718I GGTACC 1 cut(s) 472
AspA2I CCTAGG 1 cut(s) 458
AspLEI GCGC 2 cut(s) 191, 468
AspS9I GGNCC 1 cut(s) 437
AsuHPI GGTGA 2 cut(s) 115, 335
AvaII GGWCC 1 cut(s) 437
AvrII CCTAGG 1 cut(s) 458
BanI GGYRCC 1 cut(s) 472
BbsI GAAGAC 2 cut(s) 170, 339
Bbv12I GWGCWC 1 cut(s) 261
BbvCI CCTCAGC 2 cut(s) 362, 384
BbvI GCAGC 2 cut(s) 184, 367
BccI CCATC 1 cut(s) 495
BceAI ACGGC 2 cut(s) 63, 91
BciT130I CCWGG 2 cut(s) 138, 312
BfaI CTAG 2 cut(s) 66, 459
BfmI CTRYAG 1 cut(s) 201
BisI GCNGC 3 cut(s) 173, 381, 400
BlnI CCTAGG 1 cut(s) 458
BlsI GCNGC 3 cut(s) 174, 382, 401
Bme1390I CCNGG 2 cut(s) 138, 312
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 3 cut(s) 135, 438, 474
BmrFI CCNGG 2 cut(s) 138, 312
BmsI GCATC 2 cut(s) 74, 99
BpiI GAAGAC 2 cut(s) 170, 339
Bpu10I CCTNAGC 2 cut(s) 362, 384
Bsa29I ATCGAT 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 243
BsaJI CCNNGG 3 cut(s) 310, 311, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 482
Bse118I RCCGGY 2 cut(s) 191, 195
BseBI CCWGG 2 cut(s) 138, 312
BseCI ATCGAT 1 cut(s) 82
BseDI CCNNGG 3 cut(s) 310, 311, 458
BseGI GGATG 1 cut(s) 34
BseLI CCNNNNNNNGG 2 cut(s) 279, 482
BseMII CTCAG 5 cut(s) 114, 193, 375, 376, 546
BseRI GAGGAG 2 cut(s) 140, 401
BseX3I CGGCCG 1 cut(s) 193
BseXI GCAGC 2 cut(s) 184, 367
Bsh1236I CGCG 2 cut(s) 321, 468
Bsh1285I CGRYCG 2 cut(s) 82, 196
BshFI GGCC 4 cut(s) 195, 199, 360, 486
BshNI GGYRCC 1 cut(s) 472
BshVI ATCGAT 1 cut(s) 82
BsiEI CGRYCG 2 cut(s) 82, 196
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 2 cut(s) 192, 196
BslFI GGGAC 3 cut(s) 32, 197, 450
BslI CCNNNNNNNGG 2 cut(s) 279, 482
BsmFI GGGAC 3 cut(s) 32, 197, 450
BsnI GGCC 4 cut(s) 195, 199, 360, 486
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 3 cut(s) 68, 79, 544
BspACI CCGC 3 cut(s) 321, 399, 519
BspANI GGCC 4 cut(s) 195, 199, 360, 486
BspCNI CTCAG 5 cut(s) 115, 192, 375, 376, 545
BspDI ATCGAT 1 cut(s) 82
BspFNI CGCG 2 cut(s) 321, 468
BspLI GGNNCC 3 cut(s) 135, 438, 474
BspQI GCTCTTC 1 cut(s) 389
BspT107I GGYRCC 1 cut(s) 472
BsrBI CCGCTC 1 cut(s) 399
BsrFI RCCGGY 2 cut(s) 191, 195
BssAI RCCGGY 2 cut(s) 191, 195
BssECI CCNNGG 3 cut(s) 310, 311, 458
BssMI GATC 3 cut(s) 68, 79, 544
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 1 cut(s) 458
Bst2UI CCWGG 2 cut(s) 138, 312
Bst6I CTCTTC 1 cut(s) 389
BstACI GRCGYC 1 cut(s) 243
BstC8I GCNNGC 3 cut(s) 193, 197, 517
BstDEI CTNAG 5 cut(s) 123, 179, 362, 384, 532
BstF5I GGATG 1 cut(s) 34
BstFNI CGCG 2 cut(s) 321, 468
BstHHI GCGC 2 cut(s) 191, 468
BstKTI GATC 3 cut(s) 71, 82, 547
BstMBI GATC 3 cut(s) 68, 79, 544
BstMCI CGRYCG 2 cut(s) 82, 196
BstMWI GCNNNNNNNGC 1 cut(s) 84
BstNI CCWGG 2 cut(s) 138, 312
BstSCI CCNGG 2 cut(s) 136, 310
BstSFI CTRYAG 1 cut(s) 201
BstUI CGCG 2 cut(s) 321, 468
BstV1I GCAGC 2 cut(s) 184, 367
BstV2I GAAGAC 2 cut(s) 170, 339
BstZI CGGCCG 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 82
BsuRI GGCC 4 cut(s) 195, 199, 360, 486
BsuTUI ATCGAT 1 cut(s) 82
BtsCI GGATG 1 cut(s) 34
BtsI GCAGTG 1 cut(s) 119
BtsIMutI CAGTG 4 cut(s) 119, 231, 246, 415
Cac8I GCNNGC 3 cut(s) 193, 197, 517
CaiI CAGNNNCTG 1 cut(s) 186
CfoI GCGC 2 cut(s) 191, 468
Cfr10I RCCGGY 2 cut(s) 191, 195
Cfr13I GGNCC 1 cut(s) 437
ClaI ATCGAT 1 cut(s) 82
Csp6I GTAC 2 cut(s) 229, 473
CviAII CATG 1 cut(s) 239
CviQI GTAC 2 cut(s) 229, 473
DdeI CTNAG 5 cut(s) 123, 179, 362, 384, 532
DpnI GATC 3 cut(s) 70, 81, 546
DpnII GATC 3 cut(s) 68, 79, 544
EaeI YGGCCR 1 cut(s) 193
EagI CGGCCG 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
EclXI CGGCCG 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 458
Eco47I GGWCC 1 cut(s) 437
Eco52I CGGCCG 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 136, 310
EcoT14I CCWWGG 1 cut(s) 458
ErhI CCWWGG 1 cut(s) 458
FaeI CATG 1 cut(s) 242
FaiI YATR 4 cut(s) 59, 240, 353, 479
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FaqI GGGAC 3 cut(s) 32, 197, 450
FatI CATG 1 cut(s) 238
Fnu4HI GCNGC 3 cut(s) 173, 381, 400
FokI GGATG 1 cut(s) 41
FseI GGCCGGCC 1 cut(s) 199
Fsp4HI GCNGC 3 cut(s) 173, 381, 400
FspBI CTAG 2 cut(s) 66, 459
GlaI GCGC 2 cut(s) 190, 467
GluI GCNGC 3 cut(s) 173, 381, 400
HaeIII GGCC 4 cut(s) 195, 199, 360, 486
HapII CCGG 2 cut(s) 192, 196
HhaI GCGC 2 cut(s) 191, 468
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 1 cut(s) 242
Hin6I GCGC 2 cut(s) 189, 466
HinP1I GCGC 2 cut(s) 189, 466
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 2 cut(s) 369, 530
HpaII CCGG 2 cut(s) 192, 196
HphI GGTGA 2 cut(s) 115, 335
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 2 cut(s) 151, 534
Hpy8I GTNNAC 1 cut(s) 231
Hpy99I CGWCG 2 cut(s) 26, 530
HpyAV CCTTC 3 cut(s) 91, 450, 476
HpyCH4IV ACGT 2 cut(s) 243, 540
HpyCH4V TGCA 3 cut(s) 112, 117, 214
HpyF10VI GCNNNNNNNGC 1 cut(s) 84
HpyF3I CTNAG 5 cut(s) 123, 179, 362, 384, 532
HpySE526I ACGT 2 cut(s) 243, 540
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 1 cut(s) 242
HspAI GCGC 2 cut(s) 189, 466
KpnI GGTACC 1 cut(s) 476
KroI GCCGGC 2 cut(s) 191, 195
KroNI GCCGGC 2 cut(s) 193, 197
Kzo9I GATC 3 cut(s) 68, 79, 544
LguI GCTCTTC 1 cut(s) 389
LmnI GCTCC 2 cut(s) 133, 296
Lsp1109I GCAGC 2 cut(s) 184, 367
LweI GCATC 2 cut(s) 74, 99
MaeI CTAG 2 cut(s) 66, 459
MaeII ACGT 2 cut(s) 243, 540
MaeIII GTNAC 2 cut(s) 244, 323
MalI GATC 3 cut(s) 70, 81, 546
MbiI CCGCTC 1 cut(s) 399
MboI GATC 3 cut(s) 68, 79, 544
MboII GAAGA 5 cut(s) 118, 175, 344, 397, 406
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 1 cut(s) 40
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 243
MroNI GCCGGC 2 cut(s) 191, 195
MroXI GAANNNNTTC 1 cut(s) 504
MspI CCGG 2 cut(s) 192, 196
MspR9I CCNGG 2 cut(s) 138, 312
MvaI CCWGG 2 cut(s) 138, 312
MvnI CGCG 2 cut(s) 321, 468
MwoI GCNNNNNNNGC 1 cut(s) 84
NaeI GCCGGC 2 cut(s) 193, 197
NdeII GATC 3 cut(s) 68, 79, 544
NgoMIV GCCGGC 2 cut(s) 191, 195
NlaIII CATG 1 cut(s) 242
NlaIV GGNNCC 3 cut(s) 135, 438, 474
NmuCI GTSAC 2 cut(s) 244, 323
PasI CCCWGGG 1 cut(s) 311
PciSI GCTCTTC 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PdiI GCCGGC 2 cut(s) 193, 197
PdmI GAANNNNTTC 1 cut(s) 504
PfeI GAWTC 1 cut(s) 369
PkrI GCNGC 3 cut(s) 174, 382, 401
Ple19I CGATCG 1 cut(s) 82
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
PpuMI RGGWCCY 1 cut(s) 437
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 136, 310
PspGI CCWGG 2 cut(s) 136, 310
PspN4I GGNNCC 3 cut(s) 135, 438, 474
PspPI GGNCC 1 cut(s) 437
PspPPI RGGWCCY 1 cut(s) 437
PstNI CAGNNNCTG 1 cut(s) 186
PvuI CGATCG 1 cut(s) 82
RigI GGCCGGCC 1 cut(s) 199
RsaI GTAC 2 cut(s) 230, 474
RsaNI GTAC 2 cut(s) 229, 473
SapI GCTCTTC 1 cut(s) 389
SatI GCNGC 3 cut(s) 173, 381, 400
Sau3AI GATC 3 cut(s) 68, 79, 544
Sau96I GGNCC 1 cut(s) 437
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 2 cut(s) 138, 312
SduI GDGCHC 1 cut(s) 261
SfaNI GCATC 2 cut(s) 74, 99
SfcI CTRYAG 1 cut(s) 201
SinI GGWCC 1 cut(s) 437
Sse9I AATT 1 cut(s) 40
SsiI CCGC 3 cut(s) 321, 399, 519
SspMI CTAG 2 cut(s) 66, 459
StyD4I CCNGG 2 cut(s) 136, 310
StyI CCWWGG 1 cut(s) 458
TaiI ACGT 2 cut(s) 246, 543
TaqI TCGA 1 cut(s) 82
TaqII GACCGA 1 cut(s) 182
TasI AATT 1 cut(s) 40
TatI WGTACW 1 cut(s) 228
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 1 cut(s) 369
TscAI CASTG 4 cut(s) 119, 238, 253, 415
TseFI GTSAC 2 cut(s) 244, 323
TseI GCWGC 2 cut(s) 172, 380
Tsp45I GTSAC 2 cut(s) 244, 323
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 4 cut(s) 119, 238, 253, 415
VpaK11BI GGWCC 1 cut(s) 437
XmaJI CCTAGG 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 504
XspI CTAG 2 cut(s) 66, 459
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.