Rroxscaffold_6G00404360

Mal d 1-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008603
Physical Location & Seq
Reverse (-)
26895225 .. 26897261
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404360.1

Sequence Viewer

Length: 537 bp
ATGGGTTGGGTATGGAGGGACGACGACGACGACGACCAAGCAGGCGACGTTAATCCGTCTGCGGCTGACATCAGCCCCCGATCGGACGGCGACCGATGCTCCACGAGAAAGATGTATGAGGCTTTTGATTATCTTCAATTAGATGAACATGCTCAAAGGCCCGTTGAAGTGTTACAATCCAACAAAGAGTACACCGAAGATGATGTCACGGACCAGGTGATGAAAGGGTCTGTGCCTTTTGGGTCTTCAGACAATGGAGCATTTAACTTCCCTGGACTACAGAGTGATATTGATGAAATTGAACGCAACTTCTTAGGTGGGCTCAGCCGATTCTTTGAAGCTGCTGAGGATATGAAGAATGGATTCTTCAGTTCATTTGGCATTCCACACATCTTTGATGAGGGACCCTCAACTTCACTACCATCTCCGAGGAGAGAGATACCTATTGACAGCCATCGTCAGCGGGAAGCTTTTCCCAAACAAAATGGCACAAAATCTGGGGAGGTTGATCTTTCTGGGTTGGCAAGAGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

19.89

Weight (kDa)

4.35

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 62, 463
AcuI CTGAAG 2 cut(s) 231, 352
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 1 cut(s) 82
AgsI TTSAA 4 cut(s) 137, 167, 302, 338
AjnI CCWGG 2 cut(s) 213, 271
AluBI AGCT 2 cut(s) 341, 470
AluI AGCT 2 cut(s) 341, 470
AoxI GGCC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 341
ArsI GACNNNNNNTTYG 2 cut(s) 189, 221
Asp700I GAANNNNTTC 2 cut(s) 362, 471
AspS9I GGNCC 3 cut(s) 159, 211, 404
AsuHPI GGTGA 1 cut(s) 229
AvaII GGWCC 2 cut(s) 211, 404
BanII GRGCYC 1 cut(s) 324
BauI CACGAG 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 237
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 1 cut(s) 328
BccI CCATC 2 cut(s) 430, 462
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 2 cut(s) 215, 273
BfmI CTRYAG 1 cut(s) 278
BisI GCNGC 2 cut(s) 63, 342
BlpI GCTNAGC 1 cut(s) 323
BlsI GCNGC 2 cut(s) 64, 343
Bme1390I CCNGG 2 cut(s) 215, 273
Bme18I GGWCC 2 cut(s) 211, 404
BmgT120I GGNCC 3 cut(s) 159, 211, 404
BmiI GGNNCC 2 cut(s) 405, 406
BmrFI CCNGG 2 cut(s) 215, 273
BmsI GCATC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 237
BplI GAGNNNNNCTC 2 cut(s) 392, 424
Bpu10I CCTNAGC 1 cut(s) 345
Bpu1102I GCTNAGC 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 271, 428
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 82
BseBI CCWGG 2 cut(s) 215, 273
BseDI CCNNGG 2 cut(s) 271, 428
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMII CTCAG 2 cut(s) 336, 337
BseRI GAGGAG 1 cut(s) 445
BseXI GCAGC 1 cut(s) 328
Bsh1285I CGRYCG 2 cut(s) 83, 94
BshFI GGCC 1 cut(s) 160
BsiEI CGRYCG 2 cut(s) 83, 94
BslFI GGGAC 2 cut(s) 32, 417
BslI CCNNNNNNNGG 1 cut(s) 82
BsmFI GGGAC 2 cut(s) 32, 417
BsmI GAATGC 1 cut(s) 381
BsnI GGCC 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 2 cut(s) 80, 508
Bsp1720I GCTNAGC 1 cut(s) 323
BspACI CCGC 2 cut(s) 62, 463
BspANI GGCC 1 cut(s) 160
BspCNI CTCAG 2 cut(s) 336, 337
BspLI GGNNCC 2 cut(s) 405, 406
BssECI CCNNGG 2 cut(s) 271, 428
BssMI GATC 2 cut(s) 80, 508
BssSI CACGAG 1 cut(s) 103
Bst2BI CACGAG 1 cut(s) 103
Bst2UI CCWGG 2 cut(s) 215, 273
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 3 cut(s) 313, 323, 345
BstKTI GATC 2 cut(s) 83, 511
BstMBI GATC 2 cut(s) 80, 508
BstMCI CGRYCG 2 cut(s) 83, 94
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstNI CCWGG 2 cut(s) 215, 273
BstNSI RCATGY 1 cut(s) 152
BstSCI CCNGG 2 cut(s) 213, 271
BstSFI CTRYAG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 237
BsuRI GGCC 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 43
Cfr13I GGNCC 3 cut(s) 159, 211, 404
CsiI ACCWGGT 1 cut(s) 213
Csp6I GTAC 1 cut(s) 190
CviAII CATG 1 cut(s) 149
CviJI RGCY 9 cut(s) 65, 75, 122, 160, 322, 327, 341, 453, 470
CviKI_1 RGCY 9 cut(s) 65, 75, 122, 160, 322, 327, 341, 453, 470
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 3 cut(s) 313, 323, 345
DpnI GATC 2 cut(s) 82, 510
DpnII GATC 2 cut(s) 80, 508
Eco24I GRGCYC 1 cut(s) 324
Eco47I GGWCC 2 cut(s) 211, 404
Eco57I CTGAAG 2 cut(s) 231, 352
EcoO109I RGGNCCY 1 cut(s) 404
EcoRII CCWGG 2 cut(s) 213, 271
EcoT38I GRGCYC 1 cut(s) 324
FaeI CATG 1 cut(s) 152
FaiI YATR 4 cut(s) 13, 117, 150, 353
FaqI GGGAC 2 cut(s) 32, 417
FatI CATG 1 cut(s) 148
FauI CCCGC 1 cut(s) 456
Fnu4HI GCNGC 2 cut(s) 63, 342
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 2 cut(s) 63, 342
GluI GCNGC 2 cut(s) 63, 342
HaeIII GGCC 1 cut(s) 160
Hin1II CATG 1 cut(s) 152
HindIII AAGCTT 1 cut(s) 468
HinfI GANTC 2 cut(s) 330, 363
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 1 cut(s) 192
Hpy188I TCNGA 3 cut(s) 85, 250, 429
Hpy8I GTNNAC 1 cut(s) 192
Hpy99I CGWCG 5 cut(s) 26, 29, 32, 35, 50
HpyCH4IV ACGT 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
HpyF3I CTNAG 3 cut(s) 313, 323, 345
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 1 cut(s) 152
KflI GGGWCCC 1 cut(s) 404
Kzo9I GATC 2 cut(s) 80, 508
LmnI GCTCC 2 cut(s) 104, 257
LpnPI CCDG 7 cut(s) 27, 200, 227, 258, 285, 483, 501
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 1 cut(s) 86
MabI ACCWGGT 1 cut(s) 213
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 2 cut(s) 171, 205
MalI GATC 2 cut(s) 82, 510
MboI GATC 2 cut(s) 80, 508
MboII GAAGA 5 cut(s) 125, 209, 237, 358, 367
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 2 cut(s) 137, 297
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 7 cut(s) 9, 112, 340, 394, 418, 423, 496
MroXI GAANNNNTTC 2 cut(s) 362, 471
MseI TTAA 2 cut(s) 51, 264
MspA1I CMGCKG 1 cut(s) 463
MspR9I CCNGG 2 cut(s) 215, 273
Mva1269I GAATGC 1 cut(s) 381
MvaI CCWGG 2 cut(s) 215, 273
MwoI GCNNNNNNNGC 1 cut(s) 96
NdeII GATC 2 cut(s) 80, 508
NlaIII CATG 1 cut(s) 152
NlaIV GGNNCC 2 cut(s) 405, 406
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 1 cut(s) 152
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 30
PctI GAATGC 1 cut(s) 381
PdmI GAANNNNTTC 2 cut(s) 362, 471
PfeI GAWTC 2 cut(s) 330, 363
PkrI GCNGC 2 cut(s) 64, 343
Ple19I CGATCG 1 cut(s) 83
PpuMI RGGWCCY 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 404
Psp6I CCWGG 2 cut(s) 213, 271
PspGI CCWGG 2 cut(s) 213, 271
PspN4I GGNNCC 2 cut(s) 405, 406
PspPI GGNCC 3 cut(s) 159, 211, 404
PspPPI RGGWCCY 1 cut(s) 404
PvuI CGATCG 1 cut(s) 83
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
SaqAI TTAA 2 cut(s) 51, 264
SatI GCNGC 2 cut(s) 63, 342
Sau3AI GATC 2 cut(s) 80, 508
Sau96I GGNCC 3 cut(s) 159, 211, 404
ScrFI CCNGG 2 cut(s) 215, 273
SduI GDGCHC 1 cut(s) 324
SetI ASST 7 cut(s) 51, 219, 319, 343, 445, 472, 507
SexAI ACCWGGT 1 cut(s) 213
SfaNI GCATC 1 cut(s) 86
SfcI CTRYAG 1 cut(s) 278
SinI GGWCC 2 cut(s) 211, 404
Sse9I AATT 2 cut(s) 137, 297
SsiI CCGC 2 cut(s) 62, 463
StyD4I CCNGG 2 cut(s) 213, 271
TaiI ACGT 1 cut(s) 51
TaqII GACCGA 1 cut(s) 108
TasI AATT 2 cut(s) 137, 297
TatI WGTACW 1 cut(s) 189
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 330, 363
Tru1I TTAA 2 cut(s) 51, 264
Tru9I TTAA 2 cut(s) 51, 264
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 5 cut(s) 159, 236, 309, 363, 368
TspGWI ACGGA 2 cut(s) 45, 224
VpaK11BI GGWCC 2 cut(s) 211, 404
XceI RCATGY 1 cut(s) 152
XmnI GAANNNNTTC 2 cut(s) 362, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.