pycom12g03810

Mal d 1-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008603
Physical Location & Seq
Forward (+)
3682639 .. 3684421
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g03810.3

Sequence Viewer

Length: 711 bp
ATGGCATCCATTTTTGGCGGGCCGAAAACCTCCTCTCCAACTAAGCCCACAACGCTTCCCAAACCTTCCACCGACTTGAGCAAAAATCGCAGAGATTTTCTGAAGTTGGCGCGAACAGAGAGGTACAGAAGAAAGATGGGTTGGGTGTGGAGGGACGACGAAGAAGGCGACAGCAATTCTTCGGCGCTTGACATAAACCCTAGATCGGATGGCGAGCGATGCTCGACGAGAAAGGTGGTGAAGACGCAGTGCAAGACTGAGGAGGTGGAGCCTGGAAAGTTCATGCGGAAGTGCGAGAAGACTGAGCAGCTTCTCAGGGACTGCGCCGGCCGGCCTGTTGAAGTGGTGCAATCCAACAAAGAGTACACAGAAGATGACGTCACTGACGAGATGCTCAAACGGTCTGTCACCTTTGGGTCATCACAGCATGGAGCGTTTGACTTCCCAGGGTTACAAAGTGACATTGAAGACATTGAACGCAACTTTATGGGTGGCCTCAACCGATTCTTTGAAGCAGCGGAGGAGTTGAAGAGTGGCTTCTTCAGTGCTTTTGGGACTCCACACATCTTTGACGAGGGACCTTCCCGCCCTCTACCATCTAGGAGGCGAGAGGTGCCCATGGAAGGTCATCGTCAGCAGGAAGCTTTTCCTAAAGCAAGTGATGGCGACTCAGGACAAGTTGATCTCTCTGGGTTGGCGAGAGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

26.61

Weight (kDa)

5.8

Isoelectric Point (pI)

62.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 381
AccB1I GGYRCC 1 cut(s) 613
AccII CGCG 1 cut(s) 112
AciI CCGC 4 cut(s) 18, 286, 518, 586
AcoI YGGCCR 1 cut(s) 328
AcuI CTGAAG 2 cut(s) 122, 526
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 2 cut(s) 125, 365
AfiI CCNNNNNNNGG 2 cut(s) 205, 623
AgsI TTSAA 5 cut(s) 341, 467, 476, 512, 529
AjnI CCWGG 2 cut(s) 271, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 124, 156
AluBI AGCT 2 cut(s) 310, 644
AluI AGCT 2 cut(s) 310, 644
AlwNI CAGNNNCTG 1 cut(s) 321
AoxI GGCC 4 cut(s) 20, 328, 332, 493
ApeKI GCWGC 2 cut(s) 307, 515
ArsI GACNNNNNNTTYG 2 cut(s) 390, 422
Asp700I GAANNNNTTC 1 cut(s) 645
AspLEI GCGC 3 cut(s) 112, 187, 326
AspS9I GGNCC 2 cut(s) 20, 578
AsuHPI GGTGA 2 cut(s) 250, 400
AvaII GGWCC 1 cut(s) 578
BaeGI GKGCMC 1 cut(s) 618
BanI GGYRCC 1 cut(s) 613
BbsI GAAGAC 3 cut(s) 248, 305, 474
BbvI GCAGC 2 cut(s) 319, 527
BccI CCATC 4 cut(s) 130, 203, 604, 656
BciT130I CCWGG 2 cut(s) 273, 447
BfaI CTAG 2 cut(s) 201, 600
BfoI RGCGCY 1 cut(s) 188
BisI GCNGC 2 cut(s) 308, 516
BlsI GCNGC 2 cut(s) 309, 517
Bme1390I CCNGG 2 cut(s) 273, 447
Bme18I GGWCC 1 cut(s) 578
BmgT120I GGNCC 2 cut(s) 20, 578
BmiI GGNNCC 3 cut(s) 270, 579, 615
BmrFI CCNGG 2 cut(s) 273, 447
BmsI GCATC 3 cut(s) 14, 209, 381
BpiI GAAGAC 3 cut(s) 248, 305, 474
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BpuEI CTTGAG 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 3 cut(s) 445, 446, 618
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 623
Bse118I RCCGGY 2 cut(s) 326, 330
BseBI CCWGG 2 cut(s) 273, 447
BseDI CCNNGG 3 cut(s) 445, 446, 618
BseGI GGATG 2 cut(s) 5, 214
BseLI CCNNNNNNNGG 2 cut(s) 205, 623
BseMII CTCAG 4 cut(s) 249, 294, 328, 684
BseRI GAGGAG 3 cut(s) 22, 275, 536
BseSI GKGCMC 1 cut(s) 618
BseX3I CGGCCG 1 cut(s) 328
BseXI GCAGC 2 cut(s) 319, 527
Bsh1236I CGCG 1 cut(s) 112
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 4 cut(s) 22, 330, 334, 495
BshNI GGYRCC 1 cut(s) 613
BsiEI CGRYCG 1 cut(s) 331
BsiSI CCGG 2 cut(s) 327, 331
BslFI GGGAC 4 cut(s) 167, 332, 568, 591
BslI CCNNNNNNNGG 2 cut(s) 205, 623
BsmFI GGGAC 4 cut(s) 167, 332, 568, 591
BsnI GGCC 4 cut(s) 22, 330, 334, 495
Bsp1286I GDGCHC 1 cut(s) 618
Bsp143I GATC 2 cut(s) 203, 682
Bsp19I CCATGG 1 cut(s) 618
BspACI CCGC 4 cut(s) 18, 286, 518, 586
BspANI GGCC 4 cut(s) 22, 330, 334, 495
BspCNI CTCAG 4 cut(s) 250, 295, 327, 683
BspFNI CGCG 1 cut(s) 112
BspLI GGNNCC 3 cut(s) 270, 579, 615
BspT107I GGYRCC 1 cut(s) 613
BsrFI RCCGGY 2 cut(s) 326, 330
BssAI RCCGGY 2 cut(s) 326, 330
BssECI CCNNGG 3 cut(s) 445, 446, 618
BssMI GATC 2 cut(s) 203, 682
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 618
Bst2UI CCWGG 2 cut(s) 273, 447
Bst4CI ACNGT 1 cut(s) 402
Bst6I CTCTTC 1 cut(s) 524
BstACI GRCGYC 1 cut(s) 378
BstC8I GCNNGC 4 cut(s) 20, 215, 328, 332
BstDEI CTNAG 5 cut(s) 42, 258, 303, 314, 670
BstDSI CCRYGG 1 cut(s) 618
BstF5I GGATG 2 cut(s) 5, 214
BstFNI CGCG 1 cut(s) 112
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 3 cut(s) 112, 187, 326
BstKTI GATC 2 cut(s) 206, 685
BstMBI GATC 2 cut(s) 203, 682
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 4 cut(s) 52, 87, 219, 613
BstNI CCWGG 2 cut(s) 273, 447
BstSCI CCNGG 2 cut(s) 271, 445
BstSLI GKGCMC 1 cut(s) 618
BstUI CGCG 1 cut(s) 112
BstV1I GCAGC 2 cut(s) 319, 527
BstV2I GAAGAC 3 cut(s) 248, 305, 474
BstZI CGGCCG 1 cut(s) 328
BsuRI GGCC 4 cut(s) 22, 330, 334, 495
BtgI CCRYGG 1 cut(s) 618
BtgZI GCGATG 1 cut(s) 232
BtsCI GGATG 2 cut(s) 5, 214
BtsI GCAGTG 1 cut(s) 254
BtsIMutI CAGTG 3 cut(s) 254, 381, 550
Cac8I GCNNGC 4 cut(s) 20, 215, 328, 332
CaiI CAGNNNCTG 1 cut(s) 321
CfoI GCGC 3 cut(s) 112, 187, 326
Cfr10I RCCGGY 2 cut(s) 326, 330
Cfr13I GGNCC 2 cut(s) 20, 578
CseI GACGC 1 cut(s) 253
Csp6I GTAC 2 cut(s) 124, 364
CviAII CATG 3 cut(s) 283, 428, 619
CviJI RGCY 9 cut(s) 22, 46, 271, 310, 330, 334, 495, 537, 644
CviKI_1 RGCY 9 cut(s) 22, 46, 271, 310, 330, 334, 495, 537, 644
CviQI GTAC 2 cut(s) 124, 364
DdeI CTNAG 5 cut(s) 42, 258, 303, 314, 670
DpnI GATC 2 cut(s) 205, 684
DpnII GATC 2 cut(s) 203, 682
EaeI YGGCCR 1 cut(s) 328
EagI CGGCCG 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 524
EarI CTCTTC 1 cut(s) 524
EclXI CGGCCG 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 578
Eco52I CGGCCG 1 cut(s) 328
Eco57I CTGAAG 2 cut(s) 122, 526
EcoO109I RGGNCCY 1 cut(s) 578
EcoRII CCWGG 2 cut(s) 271, 445
EcoT14I CCWWGG 1 cut(s) 618
ErhI CCWWGG 1 cut(s) 618
FaeI CATG 3 cut(s) 286, 431, 622
FaiI YATR 5 cut(s) 194, 284, 429, 488, 620
FalI AAGNNNNNCTT 2 cut(s) 521, 553
FaqI GGGAC 4 cut(s) 167, 332, 568, 591
FatI CATG 3 cut(s) 282, 427, 618
FauI CCCGC 2 cut(s) 11, 593
Fnu4HI GCNGC 2 cut(s) 308, 516
FokI GGATG 1 cut(s) 221
FseI GGCCGGCC 1 cut(s) 334
Fsp4HI GCNGC 2 cut(s) 308, 516
FspBI CTAG 2 cut(s) 201, 600
GlaI GCGC 3 cut(s) 111, 186, 325
GluI GCNGC 2 cut(s) 308, 516
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 4 cut(s) 22, 330, 334, 495
HapII CCGG 2 cut(s) 327, 331
HgaI GACGC 1 cut(s) 253
HhaI GCGC 3 cut(s) 112, 187, 326
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 3 cut(s) 286, 431, 622
Hin6I GCGC 3 cut(s) 110, 185, 324
HinP1I GCGC 3 cut(s) 110, 185, 324
HindIII AAGCTT 1 cut(s) 642
HinfI GANTC 3 cut(s) 504, 556, 668
HpaII CCGG 2 cut(s) 327, 331
HphI GGTGA 2 cut(s) 250, 400
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 2 cut(s) 102, 208
Hpy188III TCNNGA 1 cut(s) 672
Hpy8I GTNNAC 1 cut(s) 366
Hpy99I CGWCG 2 cut(s) 161, 229
HpyAV CCTTC 4 cut(s) 75, 158, 591, 617
HpyCH4III ACNGT 1 cut(s) 402
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 2 cut(s) 252, 349
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 87, 219, 613
HpyF3I CTNAG 5 cut(s) 42, 258, 303, 314, 670
HpySE526I ACGT 1 cut(s) 378
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 3 cut(s) 286, 431, 622
HspAI GCGC 3 cut(s) 110, 185, 324
KroI GCCGGC 2 cut(s) 326, 330
KroNI GCCGGC 2 cut(s) 328, 332
Kzo9I GATC 2 cut(s) 203, 682
LmnI GCTCC 2 cut(s) 268, 431
Lsp1109I GCAGC 2 cut(s) 319, 527
LweI GCATC 3 cut(s) 14, 209, 381
MaeI CTAG 2 cut(s) 201, 600
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 4 cut(s) 379, 406, 450, 458
MalI GATC 2 cut(s) 205, 684
MboI GATC 2 cut(s) 203, 682
MboII GAAGA 9 cut(s) 141, 171, 173, 253, 310, 383, 479, 532, 541
MhlI GDGCHC 1 cut(s) 618
MluCI AATT 1 cut(s) 175
MlyI GAGTC 2 cut(s) 550, 662
MmeI TCCRAC 2 cut(s) 62, 378
MroNI GCCGGC 2 cut(s) 326, 330
MroXI GAANNNNTTC 1 cut(s) 645
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 2 cut(s) 327, 331
MspR9I CCNGG 2 cut(s) 273, 447
MvaI CCWGG 2 cut(s) 273, 447
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 4 cut(s) 52, 87, 219, 613
NaeI GCCGGC 2 cut(s) 328, 332
NcoI CCATGG 1 cut(s) 618
NdeII GATC 2 cut(s) 203, 682
NgoMIV GCCGGC 2 cut(s) 326, 330
NlaIII CATG 3 cut(s) 286, 431, 622
NlaIV GGNNCC 3 cut(s) 270, 579, 615
NmuCI GTSAC 3 cut(s) 379, 406, 458
PasI CCCWGGG 1 cut(s) 446
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 384
PdiI GCCGGC 2 cut(s) 328, 332
PdmI GAANNNNTTC 1 cut(s) 645
PfeI GAWTC 1 cut(s) 504
PkrI GCNGC 2 cut(s) 309, 517
PleI GAGTC 2 cut(s) 550, 662
PpsI GAGTC 2 cut(s) 550, 662
PpuMI RGGWCCY 1 cut(s) 578
Psp5II RGGWCCY 1 cut(s) 578
Psp6I CCWGG 2 cut(s) 271, 445
PspGI CCWGG 2 cut(s) 271, 445
PspN4I GGNNCC 3 cut(s) 270, 579, 615
PspPI GGNCC 2 cut(s) 20, 578
PspPPI RGGWCCY 1 cut(s) 578
PstNI CAGNNNCTG 1 cut(s) 321
RigI GGCCGGCC 1 cut(s) 334
RsaI GTAC 2 cut(s) 125, 365
RsaNI GTAC 2 cut(s) 124, 364
SatI GCNGC 2 cut(s) 308, 516
Sau3AI GATC 2 cut(s) 203, 682
Sau96I GGNCC 2 cut(s) 20, 578
SchI GAGTC 2 cut(s) 550, 662
ScrFI CCNGG 2 cut(s) 273, 447
SduI GDGCHC 1 cut(s) 618
SfaNI GCATC 3 cut(s) 14, 209, 381
SinI GGWCC 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 1 cut(s) 175
SsiI CCGC 4 cut(s) 18, 286, 518, 586
SspMI CTAG 2 cut(s) 201, 600
StyD4I CCNGG 2 cut(s) 271, 445
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 1 cut(s) 402
TaiI ACGT 1 cut(s) 381
TaqI TCGA 1 cut(s) 224
TasI AATT 1 cut(s) 175
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 1 cut(s) 504
TscAI CASTG 3 cut(s) 254, 388, 550
TseFI GTSAC 3 cut(s) 379, 406, 458
TseI GCWGC 2 cut(s) 307, 515
Tsp45I GTSAC 3 cut(s) 379, 406, 458
TspDTI ATGAA 1 cut(s) 271
TspRI CASTG 3 cut(s) 254, 388, 550
VpaK11BI GGWCC 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 645
XspI CTAG 2 cut(s) 201, 600
ZraI GACGTC 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.