pycom14g03670

Mal d 1-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008603
Physical Location & Seq
Forward (+)
3076734 .. 3078570
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g03670.1

Sequence Viewer

Length: 768 bp
ATGTGCACCAGAGAAAGTCTTGTTTTCGCTTTCCTAGAACATAAGTCATTCCACTTGTTTTGCAATCTTGGTGGGCCCAAACATCCTTTCCAATCAGGTCCACAATGCCATCTTCCCAAACCTTCCACCGACTGTAAAGCTCGCAAATATTTCTCGAGTTCGCGCGAACAGGGAGTGACAGAAGGAAAGAGAAAGATGGGGTGGGTGTGGAGGGACGACGAGGATGATGACAACAATTCAACGGCGATTGACATAAATCCTAGATCGGACGGCGATCGATGCTTGACGAGGAAGGTGGTGAAGACGCAGTGCAAGACTGAGGAGGTGGAGCCTGGAAAGTTCGTCAGGAAGTGCGAGAAGACCGAGCAGCTTCTCAGGGACTGCGCCGGCCGGCCCGTAGAAGTGGTGCAATCCAACAAGGAGTACACTGAAGATGATGTCACTGAAGAGGTGCTCAAGGGGTCTGCCACCTTTGGGTCATCACAGCACGGAGCATTTGACTTCCCAGGGCTACGCAGTGACATTGAAGACATTGAACGCAACTTTATGGGTGGCCTCAGCCGATTCTTTGAGGCTGCCGAGGAGTTGAAGAGTGGCTTCTTCAGTGCGTTTGGGAACATCTTTGATGAGGGACCTTCCTCCCCACTACCTCCTAGGAGGCGCGAGGTACCCATTGAAGGCCATCATCAGCAGGAAGCTTTTCCTAAAGCAAGTGTTAGCGACGACTCAGGACACGTTGATCTCTCTGGCTTGGCAAGAGATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

256

Amino Acids

28.61

Weight (kDa)

5.46

Isoelectric Point (pI)

63.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 667
AccB1I GGYRCC 1 cut(s) 667
AccII CGCG 3 cut(s) 163, 165, 663
AcoI YGGCCR 1 cut(s) 388
AcuI CTGAAG 3 cut(s) 450, 465, 586
AfaI GTAC 2 cut(s) 425, 669
AfiI CCNNNNNNNGG 2 cut(s) 474, 677
AflIII ACRYGT 1 cut(s) 733
AgsI TTSAA 5 cut(s) 240, 527, 536, 589, 677
AjnI CCWGG 2 cut(s) 331, 505
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 3 cut(s) 140, 370, 698
AluI AGCT 3 cut(s) 140, 370, 698
Alw21I GWGCWC 2 cut(s) 8, 456
Alw44I GTGCAC 1 cut(s) 4
AlwNI CAGNNNCTG 1 cut(s) 381
Ama87I CYCGRG 1 cut(s) 154
AoxI GGCC 5 cut(s) 74, 388, 392, 553, 679
ApaI GGGCCC 1 cut(s) 78
ApaLI GTGCAC 1 cut(s) 4
ApeKI GCWGC 2 cut(s) 367, 575
Asp700I GAANNNNTTC 1 cut(s) 699
Asp718I GGTACC 1 cut(s) 667
AspA2I CCTAGG 1 cut(s) 653
AspLEI GCGC 3 cut(s) 165, 386, 663
AspS9I GGNCC 5 cut(s) 74, 75, 98, 393, 632
AsuHPI GGTGA 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 154
AvaII GGWCC 2 cut(s) 98, 632
AvrII CCTAGG 1 cut(s) 653
BaeGI GKGCMC 2 cut(s) 8, 78
BanI GGYRCC 1 cut(s) 667
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 3 cut(s) 308, 365, 534
Bbv12I GWGCWC 2 cut(s) 8, 456
BbvCI CCTCAGC 1 cut(s) 557
BbvI GCAGC 2 cut(s) 379, 562
BccI CCATC 3 cut(s) 117, 190, 690
BceAI ACGGC 2 cut(s) 258, 286
BciT130I CCWGG 2 cut(s) 333, 507
BfaI CTAG 3 cut(s) 35, 261, 654
BisI GCNGC 2 cut(s) 368, 576
BlnI CCTAGG 1 cut(s) 653
BlsI GCNGC 2 cut(s) 369, 577
Bme1390I CCNGG 2 cut(s) 333, 507
Bme18I GGWCC 2 cut(s) 98, 632
BmeT110I CYCGRG 1 cut(s) 154
BmgT120I GGNCC 5 cut(s) 74, 75, 98, 393, 632
BmiI GGNNCC 4 cut(s) 76, 330, 633, 669
BmrFI CCNGG 2 cut(s) 333, 507
BmsI GCATC 1 cut(s) 269
BpiI GAAGAC 3 cut(s) 308, 365, 534
Bpu10I CCTNAGC 1 cut(s) 557
BpuEI CTTGAG 1 cut(s) 440
Bsa29I ATCGAT 1 cut(s) 277
BsaJI CCNNGG 4 cut(s) 505, 506, 579, 653
Bsc4I CCNNNNNNNGG 2 cut(s) 474, 677
Bse118I RCCGGY 2 cut(s) 386, 390
BseBI CCWGG 2 cut(s) 333, 507
BseCI ATCGAT 1 cut(s) 277
BseDI CCNNGG 4 cut(s) 505, 506, 579, 653
BseGI GGATG 2 cut(s) 82, 229
BseLI CCNNNNNNNGG 2 cut(s) 474, 677
BseMII CTCAG 4 cut(s) 309, 388, 571, 741
BseRI GAGGAG 2 cut(s) 335, 596
BseSI GKGCMC 2 cut(s) 8, 78
BseX3I CGGCCG 1 cut(s) 388
BseXI GCAGC 2 cut(s) 379, 562
Bsh1236I CGCG 3 cut(s) 163, 165, 663
Bsh1285I CGRYCG 2 cut(s) 277, 391
BshFI GGCC 5 cut(s) 76, 390, 394, 555, 681
BshNI GGYRCC 1 cut(s) 667
BshVI ATCGAT 1 cut(s) 277
BsiEI CGRYCG 2 cut(s) 277, 391
BsiHKAI GWGCWC 2 cut(s) 8, 456
BsiHKCI CYCGRG 1 cut(s) 154
BsiSI CCGG 2 cut(s) 387, 391
BslFI GGGAC 3 cut(s) 227, 392, 645
BslI CCNNNNNNNGG 2 cut(s) 474, 677
BsmFI GGGAC 3 cut(s) 227, 392, 645
BsnI GGCC 5 cut(s) 76, 390, 394, 555, 681
BsoBI CYCGRG 1 cut(s) 154
Bsp120I GGGCCC 1 cut(s) 74
Bsp1286I GDGCHC 3 cut(s) 8, 78, 456
Bsp143I GATC 3 cut(s) 263, 274, 739
BspANI GGCC 5 cut(s) 76, 390, 394, 555, 681
BspCNI CTCAG 4 cut(s) 310, 387, 570, 740
BspDI ATCGAT 1 cut(s) 277
BspFNI CGCG 3 cut(s) 163, 165, 663
BspLI GGNNCC 4 cut(s) 76, 330, 633, 669
BspT107I GGYRCC 1 cut(s) 667
BsrFI RCCGGY 2 cut(s) 386, 390
BssAI RCCGGY 2 cut(s) 386, 390
BssECI CCNNGG 4 cut(s) 505, 506, 579, 653
BssMI GATC 3 cut(s) 263, 274, 739
BssT1I CCWWGG 1 cut(s) 653
Bst2UI CCWGG 2 cut(s) 333, 507
Bst4CI ACNGT 1 cut(s) 134
Bst6I CTCTTC 2 cut(s) 441, 584
BstC8I GCNNGC 3 cut(s) 142, 388, 392
BstDEI CTNAG 4 cut(s) 318, 374, 557, 727
BstF5I GGATG 2 cut(s) 82, 229
BstFNI CGCG 3 cut(s) 163, 165, 663
BstHHI GCGC 3 cut(s) 165, 386, 663
BstKTI GATC 3 cut(s) 266, 277, 742
BstMBI GATC 3 cut(s) 263, 274, 739
BstMCI CGRYCG 2 cut(s) 277, 391
BstMWI GCNNNNNNNGC 1 cut(s) 279
BstNI CCWGG 2 cut(s) 333, 507
BstSCI CCNGG 2 cut(s) 331, 505
BstSLI GKGCMC 2 cut(s) 8, 78
BstUI CGCG 3 cut(s) 163, 165, 663
BstV1I GCAGC 2 cut(s) 379, 562
BstV2I GAAGAC 3 cut(s) 308, 365, 534
BstZI CGGCCG 1 cut(s) 388
Bsu15I ATCGAT 1 cut(s) 277
BsuRI GGCC 5 cut(s) 76, 390, 394, 555, 681
BsuTUI ATCGAT 1 cut(s) 277
BtsCI GGATG 2 cut(s) 82, 229
BtsI GCAGTG 2 cut(s) 314, 523
BtsIMutI CAGTG 5 cut(s) 314, 426, 441, 523, 610
Cac8I GCNNGC 3 cut(s) 142, 388, 392
CaiI CAGNNNCTG 1 cut(s) 381
CfoI GCGC 3 cut(s) 165, 386, 663
Cfr10I RCCGGY 2 cut(s) 386, 390
Cfr13I GGNCC 5 cut(s) 74, 75, 98, 393, 632
ClaI ATCGAT 1 cut(s) 277
CseI GACGC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 424, 668
CspCI CAANNNNNGTGG 4 cut(s) 41, 52, 76, 87
CviQI GTAC 2 cut(s) 424, 668
DdeI CTNAG 4 cut(s) 318, 374, 557, 727
DpnI GATC 3 cut(s) 265, 276, 741
DpnII GATC 3 cut(s) 263, 274, 739
EaeI YGGCCR 1 cut(s) 388
EagI CGGCCG 1 cut(s) 388
Eam1104I CTCTTC 2 cut(s) 441, 584
EarI CTCTTC 2 cut(s) 441, 584
EclXI CGGCCG 1 cut(s) 388
Eco130I CCWWGG 1 cut(s) 653
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 2 cut(s) 98, 632
Eco52I CGGCCG 1 cut(s) 388
Eco57I CTGAAG 3 cut(s) 450, 465, 586
Eco88I CYCGRG 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 632
EcoRII CCWGG 2 cut(s) 331, 505
EcoT14I CCWWGG 1 cut(s) 653
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 653
FaiI YATR 3 cut(s) 42, 254, 548
FalI AAGNNNNNCTT 2 cut(s) 581, 613
FaqI GGGAC 3 cut(s) 227, 392, 645
Fnu4HI GCNGC 2 cut(s) 368, 576
FokI GGATG 2 cut(s) 69, 236
FriOI GRGCYC 1 cut(s) 78
FseI GGCCGGCC 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 368, 576
FspBI CTAG 3 cut(s) 35, 261, 654
GlaI GCGC 3 cut(s) 164, 385, 662
GluI GCNGC 2 cut(s) 368, 576
HaeIII GGCC 5 cut(s) 76, 390, 394, 555, 681
HapII CCGG 2 cut(s) 387, 391
HgaI GACGC 1 cut(s) 313
HhaI GCGC 3 cut(s) 165, 386, 663
Hin6I GCGC 3 cut(s) 163, 384, 661
HinP1I GCGC 3 cut(s) 163, 384, 661
HindIII AAGCTT 1 cut(s) 696
HinfI GANTC 2 cut(s) 564, 725
HpaII CCGG 2 cut(s) 387, 391
HphI GGTGA 1 cut(s) 310
Hpy166II GTNNAC 3 cut(s) 6, 101, 426
Hpy188I TCNGA 1 cut(s) 268
Hpy188III TCNNGA 3 cut(s) 154, 346, 729
Hpy8I GTNNAC 3 cut(s) 6, 101, 426
Hpy99I CGWCG 2 cut(s) 221, 725
HpyAV CCTTC 5 cut(s) 132, 176, 286, 645, 671
HpyCH4III ACNGT 1 cut(s) 134
HpyCH4IV ACGT 1 cut(s) 735
HpyCH4V TGCA 4 cut(s) 6, 63, 312, 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 279
HpyF3I CTNAG 4 cut(s) 318, 374, 557, 727
HpySE526I ACGT 1 cut(s) 735
HspAI GCGC 3 cut(s) 163, 384, 661
KpnI GGTACC 1 cut(s) 671
KroI GCCGGC 2 cut(s) 386, 390
KroNI GCCGGC 2 cut(s) 388, 392
Kzo9I GATC 3 cut(s) 263, 274, 739
LmnI GCTCC 2 cut(s) 328, 491
Lsp1109I GCAGC 2 cut(s) 379, 562
LweI GCATC 1 cut(s) 269
MaeI CTAG 3 cut(s) 35, 261, 654
MaeII ACGT 1 cut(s) 735
MaeIII GTNAC 3 cut(s) 175, 439, 518
MalI GATC 3 cut(s) 265, 276, 741
MboI GATC 3 cut(s) 263, 274, 739
MboII GAAGA 8 cut(s) 104, 313, 370, 443, 458, 539, 592, 601
MhlI GDGCHC 3 cut(s) 8, 78, 456
MluCI AATT 1 cut(s) 235
MlyI GAGTC 1 cut(s) 719
MmeI TCCRAC 1 cut(s) 438
MroNI GCCGGC 2 cut(s) 386, 390
MroXI GAANNNNTTC 1 cut(s) 699
MspI CCGG 2 cut(s) 387, 391
MspR9I CCNGG 2 cut(s) 333, 507
MvaI CCWGG 2 cut(s) 333, 507
MvnI CGCG 3 cut(s) 163, 165, 663
MwoI GCNNNNNNNGC 1 cut(s) 279
NaeI GCCGGC 2 cut(s) 388, 392
NdeII GATC 3 cut(s) 263, 274, 739
NgoMIV GCCGGC 2 cut(s) 386, 390
NlaIV GGNNCC 4 cut(s) 76, 330, 633, 669
NmeAIII GCCGAG 1 cut(s) 604
NmuCI GTSAC 3 cut(s) 175, 439, 518
PaeR7I CTCGAG 1 cut(s) 154
PasI CCCWGGG 1 cut(s) 506
PdiI GCCGGC 2 cut(s) 388, 392
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 1 cut(s) 564
PkrI GCNGC 2 cut(s) 369, 577
Ple19I CGATCG 1 cut(s) 277
PleI GAGTC 1 cut(s) 719
PpsI GAGTC 1 cut(s) 719
PpuMI RGGWCCY 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 632
Psp6I CCWGG 2 cut(s) 331, 505
PspGI CCWGG 2 cut(s) 331, 505
PspN4I GGNNCC 4 cut(s) 76, 330, 633, 669
PspOMI GGGCCC 1 cut(s) 74
PspPI GGNCC 5 cut(s) 74, 75, 98, 393, 632
PspPPI RGGWCCY 1 cut(s) 632
PstNI CAGNNNCTG 1 cut(s) 381
PvuI CGATCG 1 cut(s) 277
RigI GGCCGGCC 1 cut(s) 394
RsaI GTAC 2 cut(s) 425, 669
RsaNI GTAC 2 cut(s) 424, 668
SatI GCNGC 2 cut(s) 368, 576
Sau3AI GATC 3 cut(s) 263, 274, 739
Sau96I GGNCC 5 cut(s) 74, 75, 98, 393, 632
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 2 cut(s) 333, 507
SduI GDGCHC 3 cut(s) 8, 78, 456
SfaNI GCATC 1 cut(s) 269
Sfr274I CTCGAG 1 cut(s) 154
SinI GGWCC 2 cut(s) 98, 632
SlaI CTCGAG 1 cut(s) 154
SmlI CTYRAG 2 cut(s) 154, 455
SmoI CTYRAG 2 cut(s) 154, 455
Sse9I AATT 1 cut(s) 235
SspI AATATT 1 cut(s) 149
SspMI CTAG 3 cut(s) 35, 261, 654
StyD4I CCNGG 2 cut(s) 331, 505
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 1 cut(s) 134
TaiI ACGT 1 cut(s) 738
TaqI TCGA 2 cut(s) 155, 277
TaqII GACCGA 1 cut(s) 377
TasI AATT 1 cut(s) 235
TatI WGTACW 1 cut(s) 423
TfiI GAWTC 1 cut(s) 564
TscAI CASTG 5 cut(s) 314, 433, 448, 523, 610
TseFI GTSAC 3 cut(s) 175, 439, 518
TseI GCWGC 2 cut(s) 367, 575
Tsp45I GTSAC 3 cut(s) 175, 439, 518
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 5 cut(s) 314, 433, 448, 523, 610
VneI GTGCAC 1 cut(s) 4
VpaK11BI GGWCC 2 cut(s) 98, 632
XhoI CTCGAG 1 cut(s) 154
XmaJI CCTAGG 1 cut(s) 653
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 3 cut(s) 35, 261, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.