Rroxscaffold_6G00404860

Mal d 1-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008603
Physical Location & Seq
Reverse (-)
27355800 .. 27358172
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404860.1

Sequence Viewer

Length: 582 bp
ATGGGTTGGGTATGGAGGGACGACGACGACGACGACCAAGCAGGCGACGTTAATCCGTCTGCGGCTGACATCAGCCCCCGATCCGACCGATGCTCCACGAGAAAGATGGTAAGGACGCAGTGCAAGACGGAGGAGGTGGAGCCTGGAAAGTTCATCAGAAAGTGCGAGAAGACCGAGGAGCTTCTCCGCGACTGCGTTGGCCGGCCCGTTGAAGTGTTACAATCCAACAAAGAGTACACTGAAGATGATGTCACGGACCAGGTGATGAAAGGGTCTGTGCCTTTTGGGTCTTCAGACAATGGAGCATTTAACTTCCCTGGACTACAGAGTGATATTGATGAAATTGAACGCAACTTCTTAGGTGGGCTCAGCCGATTCTTTGAAGCTGCTGAGGATATGAAGAATGGATTCTTCAGTTCATTTGGCATTCCACACATCTTTGATGAGGGACCCTCAACTTCACTACCATCTCCGAGGAGAGAGATACCTATTGACAGCCATCGTCAGCGGGAAGCTTTTCCCAAACAAAATGGCACAAAATCTGGGGAGGTTGATCTTTCTGGGTTGGCAAGAGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.6

Weight (kDa)

4.64

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 189
AciI CCGC 3 cut(s) 62, 187, 508
AclWI GGATC 1 cut(s) 75
AcoI YGGCCR 1 cut(s) 199
AcuI CTGAAG 3 cut(s) 261, 276, 397
AfaI GTAC 1 cut(s) 236
AgsI TTSAA 3 cut(s) 212, 347, 383
AjnI CCWGG 3 cut(s) 142, 258, 316
AluBI AGCT 3 cut(s) 181, 386, 515
AluI AGCT 3 cut(s) 181, 386, 515
AlwI GGATC 1 cut(s) 75
AoxI GGCC 2 cut(s) 199, 203
ApeKI GCWGC 1 cut(s) 386
Asp700I GAANNNNTTC 2 cut(s) 407, 516
AspS9I GGNCC 3 cut(s) 204, 256, 449
AsuHPI GGTGA 1 cut(s) 274
AvaII GGWCC 2 cut(s) 256, 449
BanII GRGCYC 1 cut(s) 369
BauI CACGAG 1 cut(s) 97
BbsI GAAGAC 2 cut(s) 176, 282
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 373
BccI CCATC 3 cut(s) 100, 475, 507
BciT130I CCWGG 3 cut(s) 144, 260, 318
BfmI CTRYAG 1 cut(s) 323
BisI GCNGC 2 cut(s) 63, 387
BlpI GCTNAGC 1 cut(s) 368
BlsI GCNGC 2 cut(s) 64, 388
Bme1390I CCNGG 3 cut(s) 144, 260, 318
Bme18I GGWCC 2 cut(s) 256, 449
BmgT120I GGNCC 3 cut(s) 204, 256, 449
BmiI GGNNCC 3 cut(s) 141, 450, 451
BmrFI CCNGG 3 cut(s) 144, 260, 318
BmsI GCATC 1 cut(s) 80
BpiI GAAGAC 2 cut(s) 176, 282
BplI GAGNNNNNCTC 2 cut(s) 437, 469
Bpu10I CCTNAGC 1 cut(s) 390
Bpu1102I GCTNAGC 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 174, 316, 473
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bse118I RCCGGY 1 cut(s) 201
BseBI CCWGG 3 cut(s) 144, 260, 318
BseDI CCNNGG 3 cut(s) 174, 316, 473
BseMII CTCAG 2 cut(s) 381, 382
BseRI GAGGAG 3 cut(s) 146, 191, 490
BseXI GCAGC 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 2 cut(s) 201, 205
BsiEI CGRYCG 1 cut(s) 88
BsiSI CCGG 1 cut(s) 202
BslFI GGGAC 2 cut(s) 32, 462
BsmFI GGGAC 2 cut(s) 32, 462
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 2 cut(s) 201, 205
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 2 cut(s) 80, 553
Bsp1720I GCTNAGC 1 cut(s) 368
BspACI CCGC 3 cut(s) 62, 187, 508
BspANI GGCC 2 cut(s) 201, 205
BspCNI CTCAG 2 cut(s) 381, 382
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 3 cut(s) 141, 450, 451
BspPI GGATC 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 201
BssAI RCCGGY 1 cut(s) 201
BssECI CCNNGG 3 cut(s) 174, 316, 473
BssMI GATC 2 cut(s) 80, 553
BssSI CACGAG 1 cut(s) 97
Bst2BI CACGAG 1 cut(s) 97
Bst2UI CCWGG 3 cut(s) 144, 260, 318
BstC8I GCNNGC 2 cut(s) 43, 203
BstDEI CTNAG 3 cut(s) 358, 368, 390
BstFNI CGCG 1 cut(s) 189
BstKTI GATC 2 cut(s) 83, 556
BstMBI GATC 2 cut(s) 80, 553
BstMCI CGRYCG 1 cut(s) 88
BstNI CCWGG 3 cut(s) 144, 260, 318
BstSCI CCNGG 3 cut(s) 142, 258, 316
BstSFI CTRYAG 1 cut(s) 323
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 373
BstV2I GAAGAC 2 cut(s) 176, 282
BsuRI GGCC 2 cut(s) 201, 205
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 2 cut(s) 125, 237
Cac8I GCNNGC 2 cut(s) 43, 203
Cfr10I RCCGGY 1 cut(s) 201
Cfr13I GGNCC 3 cut(s) 204, 256, 449
CseI GACGC 1 cut(s) 124
CsiI ACCWGGT 1 cut(s) 258
Csp6I GTAC 1 cut(s) 235
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 3 cut(s) 358, 368, 390
DpnI GATC 2 cut(s) 82, 555
DpnII GATC 2 cut(s) 80, 553
EaeI YGGCCR 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 369
Eco47I GGWCC 2 cut(s) 256, 449
Eco57I CTGAAG 3 cut(s) 261, 276, 397
EcoO109I RGGNCCY 1 cut(s) 449
EcoRII CCWGG 3 cut(s) 142, 258, 316
EcoT38I GRGCYC 1 cut(s) 369
FaiI YATR 2 cut(s) 13, 398
FaqI GGGAC 2 cut(s) 32, 462
FauI CCCGC 1 cut(s) 501
Fnu4HI GCNGC 2 cut(s) 63, 387
FriOI GRGCYC 1 cut(s) 369
FseI GGCCGGCC 1 cut(s) 205
Fsp4HI GCNGC 2 cut(s) 63, 387
GluI GCNGC 2 cut(s) 63, 387
HaeIII GGCC 2 cut(s) 201, 205
HapII CCGG 1 cut(s) 202
HgaI GACGC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 513
HinfI GANTC 2 cut(s) 375, 408
HpaII CCGG 1 cut(s) 202
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 4 cut(s) 85, 158, 295, 474
Hpy8I GTNNAC 1 cut(s) 237
Hpy99I CGWCG 5 cut(s) 26, 29, 32, 35, 50
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 1 cut(s) 123
HpyF3I CTNAG 3 cut(s) 358, 368, 390
HpySE526I ACGT 1 cut(s) 48
KflI GGGWCCC 1 cut(s) 449
KroI GCCGGC 1 cut(s) 201
KroNI GCCGGC 1 cut(s) 203
Kzo9I GATC 2 cut(s) 80, 553
LmnI GCTCC 4 cut(s) 98, 139, 178, 302
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 1 cut(s) 80
MabI ACCWGGT 1 cut(s) 258
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 2 cut(s) 216, 250
MalI GATC 2 cut(s) 82, 555
MboI GATC 2 cut(s) 80, 553
MboII GAAGA 5 cut(s) 181, 254, 282, 403, 412
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 1 cut(s) 342
MmeI TCCRAC 2 cut(s) 108, 249
MnlI CCTC 9 cut(s) 9, 124, 127, 169, 385, 439, 463, 468, 541
MroNI GCCGGC 1 cut(s) 201
MroXI GAANNNNTTC 2 cut(s) 407, 516
MseI TTAA 2 cut(s) 51, 309
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 3 cut(s) 144, 260, 318
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 3 cut(s) 144, 260, 318
MvnI CGCG 1 cut(s) 189
NaeI GCCGGC 1 cut(s) 203
NdeII GATC 2 cut(s) 80, 553
NgoMIV GCCGGC 1 cut(s) 201
NlaIV GGNNCC 3 cut(s) 141, 450, 451
NmuCI GTSAC 1 cut(s) 250
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 30
PctI GAATGC 1 cut(s) 426
PdiI GCCGGC 1 cut(s) 203
PdmI GAANNNNTTC 2 cut(s) 407, 516
PfeI GAWTC 2 cut(s) 375, 408
PkrI GCNGC 2 cut(s) 64, 388
PpuMI RGGWCCY 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 3 cut(s) 142, 258, 316
PspGI CCWGG 3 cut(s) 142, 258, 316
PspN4I GGNNCC 3 cut(s) 141, 450, 451
PspPI GGNCC 3 cut(s) 204, 256, 449
PspPPI RGGWCCY 1 cut(s) 449
RigI GGCCGGCC 1 cut(s) 205
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SaqAI TTAA 2 cut(s) 51, 309
SatI GCNGC 2 cut(s) 63, 387
Sau3AI GATC 2 cut(s) 80, 553
Sau96I GGNCC 3 cut(s) 204, 256, 449
ScrFI CCNGG 3 cut(s) 144, 260, 318
SduI GDGCHC 1 cut(s) 369
SetI ASST 9 cut(s) 51, 138, 183, 264, 364, 388, 490, 517, 552
SexAI ACCWGGT 1 cut(s) 258
SfaNI GCATC 1 cut(s) 80
SfcI CTRYAG 1 cut(s) 323
SinI GGWCC 2 cut(s) 256, 449
Sse9I AATT 1 cut(s) 342
SsiI CCGC 3 cut(s) 62, 187, 508
StyD4I CCNGG 3 cut(s) 142, 258, 316
TaiI ACGT 1 cut(s) 51
TaqII GACCGA 2 cut(s) 102, 188
TasI AATT 1 cut(s) 342
TatI WGTACW 1 cut(s) 234
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 375, 408
Tru1I TTAA 2 cut(s) 51, 309
Tru9I TTAA 2 cut(s) 51, 309
TscAI CASTG 2 cut(s) 125, 244
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 5 cut(s) 142, 281, 354, 408, 413
TspGWI ACGGA 3 cut(s) 45, 143, 269
TspRI CASTG 2 cut(s) 125, 244
VpaK11BI GGWCC 2 cut(s) 256, 449
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XmnI GAANNNNTTC 2 cut(s) 407, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.