RchiOBHm_Chr3g0477171

Mal d 1-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008603
Physical Location & Seq
Forward (+)
23003249 .. 23005702
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44250

Sequence Viewer

Length: 552 bp
ATGGGTTGGGTATGGAGGGACGACGACGTTAATCCGTCTGCGGCTGACATCAGCCCCCGATCGGACGGCGACCGATGCTCCACGAGAAAGATGGTAAGGACGCAGTGCAAGACGGAGGAGGTGGAGCCTGGAAAGTTCATCAGAAAGTGCGAGAAGACCGAGGAGCTTCTCCGCGACTGCGTTGGCCGGCCCGTTGAAGTGGTACAATCCAACAAAGAGTACACCGAAGATGATGTCACGGACCAGGTGATGAAAGGGTCTGTGCCCTTTGGATCATCAGACAATGGAGCATTTAACTTCCCTGGACTACAGAGTGATATTGATGAAATTGAACGCAACTTCTTAGGTGGGCTCAGCCGATTCTTTGAAGCTGCTGAGGATATGAAGAATGGATTCTTCAGTTCATTTGGCATTCCACTCATCTTTGATGAGGGACCCTCTACTACACTACCATCTCCGAGGAGAGAGATACCTATTGACAGCCATCGTCAGCAGGAAGATGGCACAAAATCTGGGGAGGTTGATCTTTCTGGGTTGGCAAGAGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.43

Weight (kDa)

4.56

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 174
AciI CCGC 2 cut(s) 41, 172
AclWI GGATC 1 cut(s) 280
AcoI YGGCCR 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 382
AfaI GTAC 2 cut(s) 204, 221
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 3 cut(s) 197, 332, 368
AjnI CCWGG 3 cut(s) 127, 243, 301
AluBI AGCT 2 cut(s) 166, 371
AluI AGCT 2 cut(s) 166, 371
AlwI GGATC 1 cut(s) 280
AoxI GGCC 2 cut(s) 184, 188
ApeKI GCWGC 1 cut(s) 371
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
Asp700I GAANNNNTTC 1 cut(s) 392
AspS9I GGNCC 3 cut(s) 189, 241, 434
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 2 cut(s) 241, 434
BaeGI GKGCMC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 354
BauI CACGAG 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 161
BbvCI CCTCAGC 1 cut(s) 375
BbvI GCAGC 1 cut(s) 358
BccI CCATC 4 cut(s) 85, 460, 492, 494
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 3 cut(s) 129, 245, 303
BfmI CTRYAG 1 cut(s) 308
BisI GCNGC 2 cut(s) 42, 372
BlpI GCTNAGC 1 cut(s) 353
BlsI GCNGC 2 cut(s) 43, 373
Bme1390I CCNGG 3 cut(s) 129, 245, 303
Bme18I GGWCC 2 cut(s) 241, 434
BmgT120I GGNCC 3 cut(s) 189, 241, 434
BmiI GGNNCC 3 cut(s) 126, 435, 436
BmrFI CCNGG 3 cut(s) 129, 245, 303
BmsI GCATC 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 161
BplI GAGNNNNNCTC 2 cut(s) 422, 454
Bpu10I CCTNAGC 1 cut(s) 375
Bpu1102I GCTNAGC 1 cut(s) 353
BsaJI CCNNGG 3 cut(s) 159, 301, 458
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 186
BseBI CCWGG 3 cut(s) 129, 245, 303
BseDI CCNNGG 3 cut(s) 159, 301, 458
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 2 cut(s) 366, 367
BseRI GAGGAG 3 cut(s) 131, 176, 475
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 1 cut(s) 358
Bsh1236I CGCG 1 cut(s) 174
Bsh1285I CGRYCG 2 cut(s) 62, 73
BshFI GGCC 2 cut(s) 186, 190
BsiEI CGRYCG 2 cut(s) 62, 73
BsiSI CCGG 1 cut(s) 187
BslFI GGGAC 2 cut(s) 32, 447
BslI CCNNNNNNNGG 1 cut(s) 61
BsmFI GGGAC 2 cut(s) 32, 447
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 2 cut(s) 186, 190
Bsp1286I GDGCHC 2 cut(s) 267, 354
Bsp143I GATC 3 cut(s) 59, 272, 523
Bsp1720I GCTNAGC 1 cut(s) 353
BspACI CCGC 2 cut(s) 41, 172
BspANI GGCC 2 cut(s) 186, 190
BspCNI CTCAG 2 cut(s) 366, 367
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 3 cut(s) 126, 435, 436
BspPI GGATC 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 186
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 159, 301, 458
BssMI GATC 3 cut(s) 59, 272, 523
BssSI CACGAG 1 cut(s) 82
Bst2BI CACGAG 1 cut(s) 82
Bst2UI CCWGG 3 cut(s) 129, 245, 303
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 3 cut(s) 343, 353, 375
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 3 cut(s) 62, 275, 526
BstMBI GATC 3 cut(s) 59, 272, 523
BstMCI CGRYCG 2 cut(s) 62, 73
BstMWI GCNNNNNNNGC 1 cut(s) 75
BstNI CCWGG 3 cut(s) 129, 245, 303
BstSCI CCNGG 3 cut(s) 127, 243, 301
BstSFI CTRYAG 1 cut(s) 308
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 161
BsuRI GGCC 2 cut(s) 186, 190
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 1 cut(s) 110
Cac8I GCNNGC 1 cut(s) 188
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 3 cut(s) 189, 241, 434
CseI GACGC 1 cut(s) 109
CsiI ACCWGGT 1 cut(s) 243
Csp6I GTAC 2 cut(s) 203, 220
CviQI GTAC 2 cut(s) 203, 220
DdeI CTNAG 3 cut(s) 343, 353, 375
DpnI GATC 3 cut(s) 61, 274, 525
DpnII GATC 3 cut(s) 59, 272, 523
EaeI YGGCCR 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 354
Eco47I GGWCC 2 cut(s) 241, 434
Eco57I CTGAAG 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 434
EcoRII CCWGG 3 cut(s) 127, 243, 301
EcoT38I GRGCYC 1 cut(s) 354
FaiI YATR 2 cut(s) 13, 383
FaqI GGGAC 2 cut(s) 32, 447
Fnu4HI GCNGC 2 cut(s) 42, 372
FriOI GRGCYC 1 cut(s) 354
FseI GGCCGGCC 1 cut(s) 190
Fsp4HI GCNGC 2 cut(s) 42, 372
GluI GCNGC 2 cut(s) 42, 372
HaeIII GGCC 2 cut(s) 186, 190
HapII CCGG 1 cut(s) 187
HgaI GACGC 1 cut(s) 109
HinfI GANTC 2 cut(s) 360, 393
HpaII CCGG 1 cut(s) 187
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 4 cut(s) 64, 143, 280, 459
Hpy8I GTNNAC 1 cut(s) 222
Hpy99I CGWCG 2 cut(s) 26, 29
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 108
HpyF10VI GCNNNNNNNGC 1 cut(s) 75
HpyF3I CTNAG 3 cut(s) 343, 353, 375
HpySE526I ACGT 1 cut(s) 27
KflI GGGWCCC 1 cut(s) 434
KroI GCCGGC 1 cut(s) 186
KroNI GCCGGC 1 cut(s) 188
Kzo9I GATC 3 cut(s) 59, 272, 523
LmnI GCTCC 4 cut(s) 83, 124, 163, 287
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 1 cut(s) 65
MabI ACCWGGT 1 cut(s) 243
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 235
MalI GATC 3 cut(s) 61, 274, 525
MboI GATC 3 cut(s) 59, 272, 523
MboII GAAGA 5 cut(s) 166, 239, 388, 397, 509
MhlI GDGCHC 2 cut(s) 267, 354
MluCI AATT 1 cut(s) 327
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 9 cut(s) 9, 109, 112, 154, 370, 424, 448, 453, 511
MroNI GCCGGC 1 cut(s) 186
MroXI GAANNNNTTC 1 cut(s) 392
MseI TTAA 2 cut(s) 30, 294
MspI CCGG 1 cut(s) 187
MspR9I CCNGG 3 cut(s) 129, 245, 303
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 3 cut(s) 129, 245, 303
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 75
NaeI GCCGGC 1 cut(s) 188
NdeII GATC 3 cut(s) 59, 272, 523
NgoMIV GCCGGC 1 cut(s) 186
NlaIV GGNNCC 3 cut(s) 126, 435, 436
NmuCI GTSAC 1 cut(s) 235
PctI GAATGC 1 cut(s) 411
PdiI GCCGGC 1 cut(s) 188
PdmI GAANNNNTTC 1 cut(s) 392
PfeI GAWTC 2 cut(s) 360, 393
PkrI GCNGC 2 cut(s) 43, 373
Ple19I CGATCG 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 434
Psp6I CCWGG 3 cut(s) 127, 243, 301
PspGI CCWGG 3 cut(s) 127, 243, 301
PspN4I GGNNCC 3 cut(s) 126, 435, 436
PspPI GGNCC 3 cut(s) 189, 241, 434
PspPPI RGGWCCY 1 cut(s) 434
PvuI CGATCG 1 cut(s) 62
RigI GGCCGGCC 1 cut(s) 190
RsaI GTAC 2 cut(s) 204, 221
RsaNI GTAC 2 cut(s) 203, 220
SaqAI TTAA 2 cut(s) 30, 294
SatI GCNGC 2 cut(s) 42, 372
Sau3AI GATC 3 cut(s) 59, 272, 523
Sau96I GGNCC 3 cut(s) 189, 241, 434
ScrFI CCNGG 3 cut(s) 129, 245, 303
SduI GDGCHC 2 cut(s) 267, 354
SetI ASST 8 cut(s) 30, 123, 168, 249, 349, 373, 475, 522
SexAI ACCWGGT 1 cut(s) 243
SfaNI GCATC 1 cut(s) 65
SfcI CTRYAG 1 cut(s) 308
SinI GGWCC 2 cut(s) 241, 434
Sse9I AATT 1 cut(s) 327
SsiI CCGC 2 cut(s) 41, 172
StyD4I CCNGG 3 cut(s) 127, 243, 301
TaiI ACGT 1 cut(s) 30
TaqII GACCGA 2 cut(s) 87, 173
TasI AATT 1 cut(s) 327
TatI WGTACW 1 cut(s) 219
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 2 cut(s) 360, 393
Tru1I TTAA 2 cut(s) 30, 294
Tru9I TTAA 2 cut(s) 30, 294
TscAI CASTG 1 cut(s) 110
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 5 cut(s) 127, 266, 339, 393, 398
TspGWI ACGGA 3 cut(s) 24, 128, 254
TspRI CASTG 1 cut(s) 110
VpaK11BI GGWCC 2 cut(s) 241, 434
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XmnI GAANNNNTTC 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.