MD14G1079000.v1.1

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006840
Physical Location & Seq
Reverse (-)
9026194 .. 9028550
2357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1079000.v1.1.491

Sequence Viewer

Length: 924 bp
ATGAAGATGAAGATGAAGTTGAAGCTGAAGGACAAGAATGATGAAAGAGATAAGAAAGAGAAGAGGGAGAGGCTGAGGCATGACAAATTAAAGAAGGGTAAGAATTTAGATGTTGAGAAACGTGCTGTTTCAAAGAAGGATTTAGAAAGTGATAAAAGCAAGGGCTTGTCAATAGAGAAGAGCAGCATGGGGTCTAAGAGTGGGGATCTGGAAATTATGGTGGTGGAGGATTCAGATATTTACGATTTTGATAAAGATAGAGTAGGGAGGAGTTTTGAGAAAGGACAGGTGTGGGCTATATACGATGATGACGATGGAATGCCAAGGCTTTATGGTTTGATTGATGAAGTTGTATCTGTCAAACCTTTTGAGGTGAAAATGAGTTGGTTGGATCTTCAGAATAATGGTTATGAGTGGCTTGCCTTCTCGGAAAAAATGGGATTCCATGTGTCTTGTGGGAGGTTCAAAGTTTCCAGGAAGACTTCCATTAATTCATTTAATATATTTTCTCAGCTTGTGGATTGTGAAAGGGTGGCAAGAGAGATTTATTGTATATATCCGAAGAAGGGGTCAGTGTGGGCACTTTATAGTGAAACGCCTTTTGATGCAGAGGGAAGAAACTTGTCCAGTAAAGACAAGCGTTGTTGTGACATAGTTGTGTTTCTGACAGCTTATAGTGAGATGCATGGTATGAGTATAGGGTATCTTGAGAAGGTCGATGGGTTCAAGACAGTTTTTAAAATAAGGGAGATTGGGTCTCATGCTGTTAGGTTGCTAGAAAGCAGTGATGTTAGGTTGGCATCACACCAAATTCCTTCTAGGAAGCTCTCTGGTGATGAGGCTCCAAACCTTCTCAAAGACTGCTGGGAACTTGATCCTGCTTCTCTTCCTCCGGATTTACTTACTTTTGGTTCGGGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

308

Amino Acids

35.18

Weight (kDa)

8.35

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 72 - 280 1.5e-61 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 892
AclWI GGATC 3 cut(s) 213, 399, 869
AcsI RAATTY 2 cut(s) 103, 810
AcuI CTGAAG 2 cut(s) 47, 380
AfiI CCNNNNNNNGG 1 cut(s) 566
AgsI TTSAA 4 cut(s) 22, 132, 466, 727
AjnI CCWGG 1 cut(s) 473
AluBI AGCT 4 cut(s) 25, 514, 671, 826
AluI AGCT 4 cut(s) 25, 514, 671, 826
Alw26I GTCTC 1 cut(s) 762
AlwI GGATC 3 cut(s) 213, 399, 869
Aor13HI TCCGGA 1 cut(s) 892
ApeKI GCWGC 1 cut(s) 183
ApoI RAATTY 2 cut(s) 103, 810
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
AseI ATTAAT 1 cut(s) 489
AsuHPI GGTGA 2 cut(s) 385, 845
BaeGI GKGCMC 1 cut(s) 583
BbsI GAAGAC 1 cut(s) 485
BbvCI CCTCAGC 1 cut(s) 74
BbvI GCAGC 1 cut(s) 195
BccI CCATC 2 cut(s) 308, 713
BciT130I CCWGG 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 762
BfaI CTAG 2 cut(s) 776, 819
BisI GCNGC 1 cut(s) 184
BlsI GCNGC 1 cut(s) 185
Bme1390I CCNGG 1 cut(s) 475
BmiI GGNNCC 1 cut(s) 843
BmrFI CCNGG 1 cut(s) 475
BmsI GCATC 3 cut(s) 595, 672, 809
BpiI GAAGAC 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 728
BsaI GGTCTC 1 cut(s) 762
BsaJI CCNNGG 1 cut(s) 323
BsaWI WCCGGW 1 cut(s) 892
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 1 cut(s) 566
Bse1I ACTGG 1 cut(s) 627
BseAI TCCGGA 1 cut(s) 892
BseBI CCWGG 1 cut(s) 475
BseDI CCNNGG 1 cut(s) 323
BseLI CCNNNNNNNGG 1 cut(s) 566
BseMII CTCAG 2 cut(s) 65, 524
BseNI ACTGG 1 cut(s) 627
BseRI GAGGAG 1 cut(s) 283
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 1 cut(s) 195
BseYI CCCAGC 1 cut(s) 864
BsiSI CCGG 1 cut(s) 893
BslI CCNNNNNNNGG 1 cut(s) 566
BsmAI GTCTC 1 cut(s) 762
BsmI GAATGC 1 cut(s) 324
Bso31I GGTCTC 1 cut(s) 762
Bsp1286I GDGCHC 1 cut(s) 583
Bsp13I TCCGGA 1 cut(s) 892
Bsp143I GATC 3 cut(s) 205, 391, 874
BspCNI CTCAG 2 cut(s) 66, 523
BspEI TCCGGA 1 cut(s) 892
BspLI GGNNCC 1 cut(s) 843
BspPI GGATC 3 cut(s) 213, 399, 869
BspQI GCTCTTC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 762
BsrI ACTGG 1 cut(s) 627
BssECI CCNNGG 1 cut(s) 323
BssMI GATC 3 cut(s) 205, 391, 874
BssT1I CCWWGG 1 cut(s) 323
Bst2UI CCWGG 1 cut(s) 475
Bst4CI ACNGT 1 cut(s) 733
Bst6I CTCTTC 3 cut(s) 56, 173, 891
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 3 cut(s) 74, 195, 510
BstKTI GATC 3 cut(s) 208, 394, 877
BstMAI GTCTC 1 cut(s) 762
BstMBI GATC 3 cut(s) 205, 391, 874
BstNI CCWGG 1 cut(s) 475
BstSCI CCNGG 1 cut(s) 473
BstSLI GKGCMC 1 cut(s) 583
BstV1I GCAGC 1 cut(s) 195
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 2 cut(s) 205, 391
BstYI RGATCY 2 cut(s) 205, 391
BtsI GCAGTG 1 cut(s) 790
BtsIMutI CAGTG 2 cut(s) 579, 790
Cac8I GCNNGC 1 cut(s) 420
CviAII CATG 5 cut(s) 80, 187, 446, 686, 761
DdeI CTNAG 3 cut(s) 74, 195, 510
DpnI GATC 3 cut(s) 207, 393, 876
DpnII GATC 3 cut(s) 205, 391, 874
DraI TTTAAA 1 cut(s) 739
Eam1104I CTCTTC 3 cut(s) 56, 173, 891
EarI CTCTTC 3 cut(s) 56, 173, 891
Eco130I CCWWGG 1 cut(s) 323
Eco31I GGTCTC 1 cut(s) 762
Eco57I CTGAAG 2 cut(s) 47, 380
EcoRII CCWGG 1 cut(s) 473
EcoT14I CCWWGG 1 cut(s) 323
EcoT22I ATGCAT 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 323
FaeI CATG 5 cut(s) 83, 190, 449, 689, 764
FatI CATG 5 cut(s) 79, 186, 445, 685, 760
Fnu4HI GCNGC 1 cut(s) 184
Fsp4HI GCNGC 1 cut(s) 184
FspBI CTAG 2 cut(s) 776, 819
GluI GCNGC 1 cut(s) 184
GsaI CCCAGC 1 cut(s) 868
HapII CCGG 1 cut(s) 893
Hin1II CATG 5 cut(s) 83, 190, 449, 689, 764
HinfI GANTC 2 cut(s) 230, 441
HpaII CCGG 1 cut(s) 893
HphI GGTGA 2 cut(s) 385, 845
Hpy188I TCNGA 5 cut(s) 235, 399, 430, 561, 666
Hpy188III TCNNGA 5 cut(s) 209, 707, 727, 893, 915
HpyAV CCTTC 8 cut(s) 22, 88, 130, 433, 559, 706, 825, 860
HpyCH4III ACNGT 1 cut(s) 733
HpyCH4IV ACGT 1 cut(s) 121
HpyCH4V TGCA 2 cut(s) 608, 685
HpyF3I CTNAG 3 cut(s) 74, 195, 510
HpySE526I ACGT 1 cut(s) 121
Hsp92II CATG 5 cut(s) 83, 190, 449, 689, 764
Kpn2I TCCGGA 1 cut(s) 892
Kzo9I GATC 3 cut(s) 205, 391, 874
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 1 cut(s) 847
LpnPI CCDG 9 cut(s) 194, 272, 460, 487, 640, 816, 850, 891, 906
Lsp1109I GCAGC 1 cut(s) 195
LweI GCATC 3 cut(s) 595, 672, 809
MaeI CTAG 2 cut(s) 776, 819
MaeII ACGT 1 cut(s) 121
MaeIII GTNAC 1 cut(s) 647
MalI GATC 3 cut(s) 207, 393, 876
MboI GATC 3 cut(s) 205, 391, 874
MboII GAAGA 9 cut(s) 16, 22, 73, 190, 386, 490, 574, 627, 878
MflI RGATCY 2 cut(s) 205, 391
MhlI GDGCHC 1 cut(s) 583
MluCI AATT 5 cut(s) 86, 103, 213, 490, 810
MmeI TCCRAC 1 cut(s) 369
Mph1103I ATGCAT 1 cut(s) 687
MroI TCCGGA 1 cut(s) 892
MseI TTAA 4 cut(s) 89, 489, 498, 738
MslI CAYNNNNRTG 1 cut(s) 656
MspI CCGG 1 cut(s) 893
MspR9I CCNGG 1 cut(s) 475
Mva1269I GAATGC 1 cut(s) 324
MvaI CCWGG 1 cut(s) 475
NdeII GATC 3 cut(s) 205, 391, 874
NlaIII CATG 5 cut(s) 83, 190, 449, 689, 764
NlaIV GGNNCC 1 cut(s) 843
NmuCI GTSAC 1 cut(s) 647
NsiI ATGCAT 1 cut(s) 687
PciSI GCTCTTC 1 cut(s) 173
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 324
PfeI GAWTC 2 cut(s) 230, 441
PfoI TCCNGGA 1 cut(s) 473
PkrI GCNGC 1 cut(s) 185
PshBI ATTAAT 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 473
PspFI CCCAGC 1 cut(s) 864
PspGI CCWGG 1 cut(s) 473
PspN4I GGNNCC 1 cut(s) 843
PsuI RGATCY 2 cut(s) 205, 391
RseI CAYNNNNRTG 1 cut(s) 656
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 4 cut(s) 89, 489, 498, 738
SatI GCNGC 1 cut(s) 184
Sau3AI GATC 3 cut(s) 205, 391, 874
ScrFI CCNGG 1 cut(s) 475
SduI GDGCHC 1 cut(s) 583
SfaNI GCATC 3 cut(s) 595, 672, 809
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
Sse9I AATT 5 cut(s) 86, 103, 213, 490, 810
SspMI CTAG 2 cut(s) 776, 819
StyD4I CCNGG 1 cut(s) 473
StyI CCWWGG 1 cut(s) 323
TaaI ACNGT 1 cut(s) 733
TaiI ACGT 1 cut(s) 124
TaqI TCGA 1 cut(s) 717
TasI AATT 5 cut(s) 86, 103, 213, 490, 810
TfiI GAWTC 2 cut(s) 230, 441
Tru1I TTAA 4 cut(s) 89, 489, 498, 738
Tru9I TTAA 4 cut(s) 89, 489, 498, 738
TscAI CASTG 2 cut(s) 579, 790
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 1 cut(s) 183
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 6 cut(s) 17, 23, 29, 57, 360, 483
TspRI CASTG 2 cut(s) 579, 790
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 2 cut(s) 103, 810
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XspI CTAG 2 cut(s) 776, 819
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.