Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006632

Physical Location & Seq

Genomic Coordinates Forward (+)

11525975 .. 11531440

Gene Structure 5466 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1090300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGTGGGCTCTCCGCCGTGCTTCTCTTCCTCTCAGGAACCAAGCTTTCAACATAGGAACTTCTCGTGCATGCTGTGCTAATTTGGAGCTACCAATTACTGACAGACATGAGGATGTTTGTTTTCTTGAGTCCTGTAAATCATTGCCCGATGGATGTCTGCCTTCCAAGAGACTATACCACACAACACATGTTTCTTGGAATTTCTTCACAGGCACACGCAGCCTTTCTTCACAAGCTGGTGCAAAAAGCACTGGCGAGGAAGATGATTTGGAAGATGGGTTTTCTGAACTTGAAACACCATCTAGTGCTGAAGCAATTCCAGAGAGCAGTGCAAAGGATGAAAATGAAGATGAATTAATTTCTGAACCAGAACTTTCTGAAGTTGAGGAAGACGTTAAGCCTTCCCAACATGAGATGGAGTTGTCAGAGACTGAGACTGATCCAAGTGAGAAAAGATCACCAAAGAAAGGAAACACTTTAGCACTTTACAAGGCCATATTATCTTGCCCAGGTACCTCTATTCATGGTGCTCTTGATAAGTGGGTAGAAGAAGGAAATGAAATAGACCGAGCAGAGGTATCAATGGCCATGCTCAATTTTCGTAGGCGTCAAATGTATGGGAGGGCATTACAGCTTTCAGAGTGGTTGGAGGAGCATAAGAAGATTGAATTTAATGAAAGAGATTATGCATCTCGTCTTGACTTAATTGCCAAAGTACGTGGCCTCCAGAAGGCAGAAAATTATATTGAGAAGATTCCAAAATCCTTTAGAAGTGAGGTAATCTACCGAACCCTGTTGGCGTACTGTGTCTCTACCGGAAATATGAAGAAAGCCGAAGAAGTTTTCAACAAAATGAAGGACCTAGAATTCCCAGTCACAGCATTTACTTGCAACCAGTTGCTACTCCTCTACAAGAGGCTTGACAAGAAGAAAATAGCAGATGTATTACTGTTAATGGAGAAAGAAGATGTCAAGCCAACTACCTTCACATATAAAGTCCTAATAGATACAAAAGGCCAGTCAAATGACATAACTGGAATGGAGCAAGTTTATGAGACAATGAAGGCGGAAGGCATTGAGCCAGACCTGAGCACAAAAGCCACCTTGGCTAGACACTATGCCGCTAGTGGGCTTAAAGAAAAAGCTCAGGCGGTTTTGAAGGACATGGAATCTGGAAACTTAAAACAGAATCGTTGGGCTTGTCAATCTTTGCTTCCTCTTTATGCTGAGTTGGGAGATGCCAACGAAGTAGGGAGAGTTTGGAAGGTTTGTGAATCAAATCCTCTGTTTGTAGAGTGTTTGGCTGCAATCGAAGCATGGGGAAAGTTGAACAAGATTGAAGAAGCAGAAGCAGTTTTTGATAAGCTGTTAAAATCAGGGAAGAAACTTTCTTCCAAGCATTATTCTGTGATGTTGAAGGTGTATGCAAACAATAAAATGCTTGACAAGGGTAAAGATCTTGTCAAGCGTATGGCAGATGACAATTGCGAGATTGGCCCCTTAACTTGGGATGCACTTGTGAAACTCTATGTGGAAGCAGGTGAGGTTGAAAAGGCTGATTCTATTTTGCAGAAGGCTGCCCAGAACAACCGAAAGAAGCCAATGTTCACGTCTTATATGACAATTATGGAACAATATTCGAAGAGGGGGGACGTCCATAACGCAGAAAAGATATTTTATAGGATGAGACAGGCTGGTTATACGGCTCGGCTTCGTCAGTTCCATTCTCTTATTCAGGCCTATATAAACGCCAAGGCTCCGGCTTATGGAATCTGGGAGAGGATGAAGGCAGATAATATCTTTCCCAACAAATCTGCACTGGTACAGTTGGCACAAGTTGATCCATTTAAGAAGACAGCGGTGTCGGATTTGCTTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0031967 GO:0031975 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_3

Protein Analysis

627

Amino Acids

70.98

Weight (kDa)

5.99

Isoelectric Point (pI)

48.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	257 - 420	4.4e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	258 - 301	6e-07	PPR repeat family
PPR_long	PF17177	441 - 555	5.5e-06	Pentacotripeptide-repeat region of PRORP

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15480 AT1G80270 AT1G80270 AT1G80270 AT1G80270 AT1G80270
fragaria_vesca	FvH4_6g25350
malus_domestica	MD12G1096000.v1.1 MD14G1090300.v1.1
prunus_persica	Prupe.7G034000_v2.0.a1 Prupe.7G034000_v2.0.a1
pyrus_communis	pycom12g08300 pycom14g08090
rosa_chinensis	RchiOBHm_Chr2g0125771
rosa_laevigata	RLG00000018879
rosa_multiflora	Rmu_sc0005557.1_g000002 Rmu_sc0026259.1_g000001 Rmu_sc0026260.1_g000001 Rmu_ssc0000055.1_g000010
rosa_roxburghii	Rroxscaffold_2G00117730
rosa_rugosa	Rorug02G0259300
rosa_samantha	Rh2AG317300 Rh2BG325300 Rh2CG307100 Rh2DG343300
rosa_wichuraiana	Rw2G025670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1529
AasI	GACNNNNNNGTC	1 cut(s)	1861
AatII	GACGTC	1 cut(s)	1656
Acc36I	ACCTGC	1 cut(s)	1529
Acc65I	GGTACC	1 cut(s)	512
AccB1I	GGYRCC	1 cut(s)	512
AciI	CCGC	5 cut(s)	13, 1067, 1122, 1151, 1859
AclWI	GGATC	2 cut(s)	434, 1835
AcoI	YGGCCR	1 cut(s)	585
AcsI	RAATTY	3 cut(s)	199, 668, 866
AcuI	CTGAAG	2 cut(s)	330, 399
AcyI	GRCGYC	2 cut(s)	607, 1653
AfaI	GTAC	4 cut(s)	514, 717, 803, 1824
AfiI	CCNNNNNNNGG	6 cut(s)	467, 574, 730, 1128, 1506, 1766
AflIII	ACRYGT	1 cut(s)	187
AgsI	TTSAA	9 cut(s)	49, 293, 668, 847, 1159, 1330, 1340, 1417, 1550
AjiI	CACGTC	1 cut(s)	1611
AjnI	CCWGG	1 cut(s)	508
AluBI	AGCT	6 cut(s)	44, 88, 236, 634, 1145, 1366
AluI	AGCT	6 cut(s)	44, 88, 236, 634, 1145, 1366
Alw21I	GWGCWC	2 cut(s)	532, 1094
Alw26I	GTCTC	6 cut(s)	163, 422, 428, 814, 1049, 1681
AlwI	GGATC	2 cut(s)	434, 1835
AlwNI	CAGNNNCTG	1 cut(s)	431
AoxI	GGCC	6 cut(s)	492, 585, 721, 1015, 1495, 1737
ApeKI	GCWGC	3 cut(s)	219, 1304, 1577
ApoI	RAATTY	3 cut(s)	199, 668, 866
ArsI	GACNNNNNNTTYG	2 cut(s)	1004, 1036
AseI	ATTAAT	1 cut(s)	356
Asp700I	GAANNNNTTC	2 cut(s)	203, 315
Asp718I	GGTACC	1 cut(s)	512
AspS9I	GGNCC	2 cut(s)	859, 1496
AsuHPI	GGTGA	2 cut(s)	450, 1553
AsuII	TTCGAA	1 cut(s)	1640
AvaII	GGWCC	1 cut(s)	859
BalI	TGGCCA	1 cut(s)	587
BanI	GGYRCC	1 cut(s)	512
BanII	GRGCYC	1 cut(s)	10
BauI	CACGAG	1 cut(s)	63
BbsI	GAAGAC	2 cut(s)	396, 1859
Bbv12I	GWGCWC	2 cut(s)	532, 1094
BbvI	GCAGC	3 cut(s)	231, 1291, 1564
BccI	CCATC	4 cut(s)	143, 269, 307, 409
BceAI	ACGGC	1 cut(s)	1719
BciT130I	CCWGG	1 cut(s)	510
BcoDI	GTCTC	6 cut(s)	163, 422, 428, 814, 1049, 1681
BfaI	CTAG	4 cut(s)	303, 863, 1110, 1125
BfuAI	ACCTGC	1 cut(s)	1529
BglI	GCCNNNNNGGC	1 cut(s)	1106
BglII	AGATCT	1 cut(s)	1456
BisI	GCNGC	4 cut(s)	220, 1122, 1305, 1578
BlsI	GCNGC	4 cut(s)	221, 1123, 1306, 1579
Bme1390I	CCNGG	1 cut(s)	510
Bme18I	GGWCC	1 cut(s)	859
BmgBI	CACGTC	1 cut(s)	1611
BmgT120I	GGNCC	2 cut(s)	859, 1496
BmiI	GGNNCC	4 cut(s)	38, 514, 1498, 1758
BmrFI	CCNGG	1 cut(s)	510
BmrI	ACTGGG	1 cut(s)	866
BmsI	GCATC	3 cut(s)	698, 1228, 1501
BmuI	ACTGGG	1 cut(s)	866
BpiI	GAAGAC	2 cut(s)	396, 1859
BpmI	CTGGAG	1 cut(s)	710
Bpu10I	CCTNAGC	2 cut(s)	1088, 1146
Bpu14I	TTCGAA	1 cut(s)	1640
BpuEI	CTTGAG	1 cut(s)	146
BsaAI	YACGTR	1 cut(s)	719
BsaHI	GRCGYC	2 cut(s)	607, 1653
BsaJI	CCNNGG	3 cut(s)	508, 1104, 1752
BsaWI	WCCGGW	1 cut(s)	815
BsaXI	ACNNNNNCTCC	2 cut(s)	708, 738
Bsc4I	CCNNNNNNNGG	6 cut(s)	467, 574, 730, 1128, 1506, 1766
Bse1I	ACTGG	6 cut(s)	256, 872, 895, 1018, 1039, 1824
Bse3DI	GCAATG	1 cut(s)	140
BseBI	CCWGG	1 cut(s)	510
BseDI	CCNNGG	3 cut(s)	508, 1104, 1752
BseGI	GGATG	6 cut(s)	118, 158, 343, 1516, 1689, 1788
BseLI	CCNNNNNNNGG	6 cut(s)	467, 574, 730, 1128, 1506, 1766
BseMI	GCAATG	1 cut(s)	140
BseMII	CTCAG	5 cut(s)	46, 423, 1079, 1160, 1218
BseNI	ACTGG	6 cut(s)	256, 872, 895, 1018, 1039, 1824
BseRI	GAGGAG	2 cut(s)	665, 896
BseXI	GCAGC	3 cut(s)	231, 1291, 1564
BsgI	GTGCAG	1 cut(s)	1800
BshFI	GGCC	6 cut(s)	494, 587, 723, 1017, 1497, 1739
BshNI	GGYRCC	1 cut(s)	512
BsiHKAI	GWGCWC	2 cut(s)	532, 1094
BsiSI	CCGG	2 cut(s)	816, 1760
BslFI	GGGAC	1 cut(s)	1664
BslI	CCNNNNNNNGG	6 cut(s)	467, 574, 730, 1128, 1506, 1766
BsmAI	GTCTC	6 cut(s)	163, 422, 428, 814, 1049, 1681
BsmFI	GGGAC	1 cut(s)	1664
BsnI	GGCC	6 cut(s)	494, 587, 723, 1017, 1497, 1739
Bsp119I	TTCGAA	1 cut(s)	1640
Bsp1286I	GDGCHC	3 cut(s)	10, 532, 1094
Bsp143I	GATC	4 cut(s)	439, 455, 1456, 1840
BspACI	CCGC	5 cut(s)	13, 1067, 1122, 1151, 1859
BspANI	GGCC	6 cut(s)	494, 587, 723, 1017, 1497, 1739
BspCNI	CTCAG	5 cut(s)	45, 424, 1080, 1159, 1219
BspLI	GGNNCC	4 cut(s)	38, 514, 1498, 1758
BspMI	ACCTGC	1 cut(s)	1529
BspPI	GGATC	2 cut(s)	434, 1835
BspT104I	TTCGAA	1 cut(s)	1640
BspT107I	GGYRCC	1 cut(s)	512
BsrDI	GCAATG	1 cut(s)	140
BsrI	ACTGG	6 cut(s)	256, 872, 895, 1018, 1039, 1824
BssECI	CCNNGG	3 cut(s)	508, 1104, 1752
BssMI	GATC	4 cut(s)	439, 455, 1456, 1840
BssNI	GRCGYC	2 cut(s)	607, 1653
BssSI	CACGAG	1 cut(s)	63
BssT1I	CCWWGG	2 cut(s)	1104, 1752
Bst2BI	CACGAG	1 cut(s)	63
Bst2UI	CCWGG	1 cut(s)	510
Bst4CI	ACNGT	3 cut(s)	806, 951, 1827
Bst6I	CTCTTC	2 cut(s)	30, 1637
BstACI	GRCGYC	2 cut(s)	607, 1653
BstAPI	GCANNNNNTGC	1 cut(s)	74
BstBAI	YACGTR	1 cut(s)	719
BstBI	TTCGAA	1 cut(s)	1640
BstC8I	GCNNGC	1 cut(s)	70
BstDEI	CTNAG	5 cut(s)	32, 432, 1088, 1146, 1227
BstENI	CCTNNNNNAGG	1 cut(s)	728
BstF5I	GGATG	6 cut(s)	118, 158, 343, 1516, 1689, 1788
BstKTI	GATC	4 cut(s)	442, 458, 1459, 1843
BstMAI	GTCTC	6 cut(s)	163, 422, 428, 814, 1049, 1681
BstMBI	GATC	4 cut(s)	439, 455, 1456, 1840
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 219, 1106, 1313, 1494
BstNI	CCWGG	1 cut(s)	510
BstNSI	RCATGY	2 cut(s)	72, 191
BstSCI	CCNGG	1 cut(s)	508
BstV1I	GCAGC	3 cut(s)	231, 1291, 1564
BstV2I	GAAGAC	2 cut(s)	396, 1859
BstX2I	RGATCY	1 cut(s)	1456
BstYI	RGATCY	1 cut(s)	1456
BsuRI	GGCC	6 cut(s)	494, 587, 723, 1017, 1497, 1739
BtrI	CACGTC	1 cut(s)	1611
BtsCI	GGATG	6 cut(s)	118, 158, 343, 1516, 1689, 1788
BtsI	GCAGTG	1 cut(s)	334
BtsIMutI	CAGTG	3 cut(s)	249, 334, 1817
BveI	ACCTGC	1 cut(s)	1529
Cac8I	GCNNGC	1 cut(s)	70
CaiI	CAGNNNCTG	1 cut(s)	431
Cfr13I	GGNCC	2 cut(s)	859, 1496
CseI	GACGC	1 cut(s)	596
Csp6I	GTAC	4 cut(s)	513, 716, 802, 1823
CspCI	CAANNNNNGTGG	2 cut(s)	700, 735
CviAII	CATG	8 cut(s)	69, 107, 188, 410, 524, 589, 1165, 1317
CviQI	GTAC	4 cut(s)	513, 716, 802, 1823
DdeI	CTNAG	5 cut(s)	32, 432, 1088, 1146, 1227
DpnI	GATC	4 cut(s)	441, 457, 1458, 1842
DpnII	GATC	4 cut(s)	439, 455, 1456, 1840
DrdI	GACNNNNNNGTC	1 cut(s)	1861
DseDI	GACNNNNNNGTC	1 cut(s)	1861
EaeI	YGGCCR	1 cut(s)	585
Eam1104I	CTCTTC	2 cut(s)	30, 1637
EarI	CTCTTC	2 cut(s)	30, 1637
EciI	GGCGGA	1 cut(s)	1082
Eco130I	CCWWGG	2 cut(s)	1104, 1752
Eco147I	AGGCCT	1 cut(s)	1739
Eco24I	GRGCYC	1 cut(s)	10
Eco47I	GGWCC	1 cut(s)	859
Eco57I	CTGAAG	2 cut(s)	330, 399
EcoNI	CCTNNNNNAGG	1 cut(s)	728
EcoO109I	RGGNCCY	1 cut(s)	859
EcoRI	GAATTC	1 cut(s)	866
EcoRII	CCWGG	1 cut(s)	508
EcoT14I	CCWWGG	2 cut(s)	1104, 1752
EcoT22I	ATGCAT	1 cut(s)	691
EcoT38I	GRGCYC	1 cut(s)	10
ErhI	CCWWGG	2 cut(s)	1104, 1752
FaeI	CATG	8 cut(s)	72, 110, 191, 413, 527, 592, 1168, 1320
FaqI	GGGAC	1 cut(s)	1664
FatI	CATG	8 cut(s)	68, 106, 187, 409, 523, 588, 1164, 1316
Fnu4HI	GCNGC	4 cut(s)	220, 1122, 1305, 1578
FokI	GGATG	6 cut(s)	125, 165, 350, 1523, 1696, 1795
FriOI	GRGCYC	1 cut(s)	10
Fsp4HI	GCNGC	4 cut(s)	220, 1122, 1305, 1578
FspBI	CTAG	4 cut(s)	303, 863, 1110, 1125
GluI	GCNGC	4 cut(s)	220, 1122, 1305, 1578
GsuI	CTGGAG	1 cut(s)	710
HaeIII	GGCC	6 cut(s)	494, 587, 723, 1017, 1497, 1739
HapII	CCGG	2 cut(s)	816, 1760
HgaI	GACGC	1 cut(s)	596
Hin1I	GRCGYC	2 cut(s)	607, 1653
Hin1II	CATG	8 cut(s)	72, 110, 191, 413, 527, 592, 1168, 1320
HindIII	AAGCTT	1 cut(s)	42
HinfI	GANTC	7 cut(s)	128, 754, 1169, 1189, 1274, 1559, 1770
HpaII	CCGG	2 cut(s)	816, 1760
HphI	GGTGA	2 cut(s)	450, 1553
Hpy166II	GTNNAC	1 cut(s)	1608
Hpy188I	TCNGA	6 cut(s)	286, 364, 379, 427, 640, 1867
Hpy188III	TCNNGA	7 cut(s)	34, 125, 320, 533, 698, 727, 1173
Hpy8I	GTNNAC	1 cut(s)	1608
HpyCH4III	ACNGT	3 cut(s)	806, 951, 1827
HpyCH4IV	ACGT	4 cut(s)	393, 718, 1610, 1653
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 219, 1106, 1313, 1494
HpyF3I	CTNAG	5 cut(s)	32, 432, 1088, 1146, 1227
HpySE526I	ACGT	4 cut(s)	393, 718, 1610, 1653
Hsp92I	GRCGYC	2 cut(s)	607, 1653
Hsp92II	CATG	8 cut(s)	72, 110, 191, 413, 527, 592, 1168, 1320
KpnI	GGTACC	1 cut(s)	516
Kzo9I	GATC	4 cut(s)	439, 455, 1456, 1840
LmnI	GCTCC	4 cut(s)	85, 652, 1042, 1762
Lsp1109I	GCAGC	3 cut(s)	231, 1291, 1564
LweI	GCATC	3 cut(s)	698, 1228, 1501
MaeI	CTAG	4 cut(s)	303, 863, 1110, 1125
MaeII	ACGT	4 cut(s)	393, 718, 1610, 1653
MaeIII	GTNAC	1 cut(s)	874
MalI	GATC	4 cut(s)	441, 457, 1458, 1842
MboI	GATC	4 cut(s)	439, 455, 1456, 1840
MfeI	CAATTG	1 cut(s)	1483
MflI	RGATCY	1 cut(s)	1456
MhlI	GDGCHC	3 cut(s)	10, 532, 1094
MlsI	TGGCCA	1 cut(s)	587
MluNI	TGGCCA	1 cut(s)	587
MlyI	GAGTC	1 cut(s)	137
MmeI	TCCRAC	2 cut(s)	627, 1845
Mox20I	TGGCCA	1 cut(s)	587
Mph1103I	ATGCAT	1 cut(s)	691
MroXI	GAANNNNTTC	2 cut(s)	203, 315
MscI	TGGCCA	1 cut(s)	587
MslI	CAYNNNNRTG	1 cut(s)	111
Msp20I	TGGCCA	1 cut(s)	587
MspA1I	CMGCKG	1 cut(s)	1859
MspI	CCGG	2 cut(s)	816, 1760
MspR9I	CCNGG	1 cut(s)	510
MunI	CAATTG	1 cut(s)	1483
MvaI	CCWGG	1 cut(s)	510
MwoI	GCNNNNNNNGC	5 cut(s)	74, 219, 1106, 1313, 1494
NdeII	GATC	4 cut(s)	439, 455, 1456, 1840
NlaIII	CATG	8 cut(s)	72, 110, 191, 413, 527, 592, 1168, 1320
NlaIV	GGNNCC	4 cut(s)	38, 514, 1498, 1758
NmeAIII	GCCGAG	1 cut(s)	1687
NmuCI	GTSAC	1 cut(s)	874
NsiI	ATGCAT	1 cut(s)	691
NspI	RCATGY	2 cut(s)	72, 191
NspV	TTCGAA	1 cut(s)	1640
PaeI	GCATGC	1 cut(s)	72
PaqCI	CACCTGC	1 cut(s)	1529
PceI	AGGCCT	1 cut(s)	1739
PciI	ACATGT	1 cut(s)	187
PdmI	GAANNNNTTC	2 cut(s)	203, 315
PfeI	GAWTC	6 cut(s)	754, 1169, 1189, 1274, 1559, 1770
PkrI	GCNGC	4 cut(s)	221, 1123, 1306, 1579
PleI	GAGTC	1 cut(s)	136
PpsI	GAGTC	1 cut(s)	136
Ppu21I	YACGTR	1 cut(s)	719
PpuMI	RGGWCCY	1 cut(s)	859
PscI	ACATGT	1 cut(s)	187
PshBI	ATTAAT	1 cut(s)	356
Psp5II	RGGWCCY	1 cut(s)	859
Psp6I	CCWGG	1 cut(s)	508
PspGI	CCWGG	1 cut(s)	508
PspN4I	GGNNCC	4 cut(s)	38, 514, 1498, 1758
PspPI	GGNCC	2 cut(s)	859, 1496
PspPPI	RGGWCCY	1 cut(s)	859
PstNI	CAGNNNCTG	1 cut(s)	431
PsuI	RGATCY	1 cut(s)	1456
RsaI	GTAC	4 cut(s)	514, 717, 803, 1824
RsaNI	GTAC	4 cut(s)	513, 716, 802, 1823
RseI	CAYNNNNRTG	1 cut(s)	111
SatI	GCNGC	4 cut(s)	220, 1122, 1305, 1578
Sau3AI	GATC	4 cut(s)	439, 455, 1456, 1840
Sau96I	GGNCC	2 cut(s)	859, 1496
SchI	GAGTC	1 cut(s)	137
ScrFI	CCNGG	1 cut(s)	510
SduI	GDGCHC	3 cut(s)	10, 532, 1094
SfaNI	GCATC	3 cut(s)	698, 1228, 1501
SfuI	TTCGAA	1 cut(s)	1640
SinI	GGWCC	1 cut(s)	859
SmiMI	CAYNNNNRTG	1 cut(s)	111
SmlI	CTYRAG	1 cut(s)	125
SmoI	CTYRAG	1 cut(s)	125
SphI	GCATGC	1 cut(s)	72
SseBI	AGGCCT	1 cut(s)	1739
SsiI	CCGC	5 cut(s)	13, 1067, 1122, 1151, 1859
SspI	AATATT	1 cut(s)	1637
SspMI	CTAG	4 cut(s)	303, 863, 1110, 1125
StuI	AGGCCT	1 cut(s)	1739
StyD4I	CCNGG	1 cut(s)	508
StyI	CCWWGG	2 cut(s)	1104, 1752
TaaI	ACNGT	3 cut(s)	806, 951, 1827
TaiI	ACGT	4 cut(s)	396, 721, 1613, 1656
TaqI	TCGA	2 cut(s)	1311, 1640
TaqII	GACCGA	1 cut(s)	582
TauI	GCSGC	1 cut(s)	1124
TfiI	GAWTC	6 cut(s)	754, 1169, 1189, 1274, 1559, 1770
TscAI	CASTG	3 cut(s)	256, 334, 1824
TseFI	GTSAC	1 cut(s)	874
TseI	GCWGC	3 cut(s)	219, 1304, 1577
Tsp45I	GTSAC	1 cut(s)	874
TspRI	CASTG	3 cut(s)	256, 334, 1824
VpaK11BI	GGWCC	1 cut(s)	859
VspI	ATTAAT	1 cut(s)	356
XagI	CCTNNNNNAGG	1 cut(s)	728
XapI	RAATTY	3 cut(s)	199, 668, 866
XceI	RCATGY	2 cut(s)	72, 191
XmnI	GAANNNNTTC	2 cut(s)	203, 315
XspI	CTAG	4 cut(s)	303, 863, 1110, 1125
ZraI	GACGTC	1 cut(s)	1654
Zsp2I	ATGCAT	1 cut(s)	691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.