MD14G1122100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012437
Physical Location & Seq
Forward (+)
19687503 .. 19688447
945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1122100.v1.1.491

Sequence Viewer

Length: 945 bp
ATGGAGGTTGGTGTGTATAGCTCGAGCGGTAAGGAGCACCTATCCTTAACCCCGCGTCGAAAATCAGATCAAGACTTCGGCTCTCGTTATGCCCTTCTAAAGAAAGCTGATCAAGAGCACAAAGTAATAACTCAATCTAAAGCAACGGAAGTTGGAGGAGCTTATAGCGTTAGTGCTAAAGAGAATTTATCCTCAAACCTACGGCTGCGCTCACAGCAAGAGTTTAACTCTCGTCCTGCCCCTCAGCTGAATCAAAAGAAAATTGATCAAGAGCATAAAGCAATAAACCAATCTAAAGTAATGGACGGAGGAGGAGCTTATAGCGTTGGGGCCAAAGAGCACTTATCCTCGAACCCTCGTCGACGCTCGCAGCAAGGGTTCGACTCTCGTCCCACCTCGAATCAAAATAAAGCTCAAGACTCCAAAGCAAATCAACCCAAAGTTGTAGCAGCCAATAAGCTTAAGCCTGTATGGGATTGTGGCAGCTCACTATACGACTCGTTCGAGCTCGACTCGTTCAAACGCCAACTGGACTCGGCCATATCATGCAGAACCATGTCAATGCCTCATCTATCCGACCGCCGAGCTCCTCCTCCGCCGCCGCAACCAGCCATCACAGCCTCCAGCAAAAAACCCTCATCAAACAAGCTCTCCCGTTCGATTCAAAAGCTCCTGCGCTCTGTGTTCAAGCCTAATACCAAACCAAGTAACAGTAACAATTCTGGTGTCGTTTTTAAAGTCCAAGATCATCATCAAGCAAATACCAAGGATGGATTTTACGTTGTGTACAACACGACAGGTGCACTTACGACAATTCCAGAAATGATTCCTCACGAGTTTGATTTCGGTGGTCTCTCCCCAGAGATTGGGGCGTTCGTAAGAAGAACGGGATCAGAGCGATTTACAGCTTCGAGGACTGCTAATATTGGTATTTCATGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

315

Amino Acids

34.53

Weight (kDa)

9.88

Isoelectric Point (pI)

63.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41810 AT5G41810
fragaria_vesca FvH4_5g04150
malus_domestica MD06G1103200.v1.1 MD14G1122100.v1.1
prunus_persica Prupe.5G116000_v2.0.a1
pyrus_communis pycom06g09780
rosa_chinensis RchiOBHm_Chr7g0195351
rosa_laevigata RLG00000004128
rosa_multiflora Rmu_co8178312.1_g000001 Rmu_sc0005377.1_g000004 Rmu_sc0007366.1_g000003
rosa_roxburghii Rroxscaffold_3G00260560
rosa_rugosa Rorug07G0022700
rosa_samantha Rh7AG149900 Rh7BG151300 Rh7CG155100 Rh7DG151000
rosa_wichuraiana Rw7G012790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 866
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 361
AccII CGCG 1 cut(s) 55
AciI CCGC 6 cut(s) 27, 53, 580, 596, 599, 602
AclWI GGATC 1 cut(s) 898
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 1 cut(s) 184
AfaI GTAC 1 cut(s) 788
AfiI CCNNNNNNNGG 1 cut(s) 866
AflII CTTAAG 1 cut(s) 461
AgsI TTSAA 3 cut(s) 520, 665, 688
Alw21I GWGCWC 6 cut(s) 39, 120, 342, 510, 589, 805
Alw26I GTCTC 1 cut(s) 857
Alw44I GTGCAC 1 cut(s) 801
AlwI GGATC 1 cut(s) 898
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 2 cut(s) 330, 537
ApaLI GTGCAC 1 cut(s) 801
ApeKI GCWGC 4 cut(s) 205, 370, 449, 483
ApoI RAATTY 1 cut(s) 184
Asp700I GAANNNNTTC 1 cut(s) 825
AspLEI GCGC 2 cut(s) 210, 678
AspS9I GGNCC 1 cut(s) 330
AvaI CYCGRG 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 805
BanII GRGCYC 2 cut(s) 510, 589
BauI CACGAG 1 cut(s) 833
Bbv12I GWGCWC 6 cut(s) 39, 120, 342, 510, 589, 805
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 4 cut(s) 192, 382, 461, 495
BccI CCATC 2 cut(s) 620, 764
BceAI ACGGC 1 cut(s) 218
BclI TGATCA 2 cut(s) 109, 265
BcoDI GTCTC 1 cut(s) 857
BfrI CTTAAG 1 cut(s) 461
BisI GCNGC 6 cut(s) 206, 371, 450, 484, 599, 602
BlsI GCNGC 6 cut(s) 207, 372, 451, 485, 600, 603
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 330
BmiI GGNNCC 1 cut(s) 331
BoxI GACNNNNGTC 1 cut(s) 387
BplI GAGNNNNNCTC 4 cut(s) 212, 244, 497, 529
BpmI CTGGAG 1 cut(s) 607
Bpu10I CCTNAGC 1 cut(s) 243
BpuEI CTTGAG 1 cut(s) 399
BsaBI GATNNNNATC 1 cut(s) 750
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 1 cut(s) 765
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bsc4I CCNNNNNNNGG 1 cut(s) 866
Bse1I ACTGG 1 cut(s) 534
Bse8I GATNNNNATC 1 cut(s) 750
BseDI CCNNGG 1 cut(s) 765
BseGI GGATG 1 cut(s) 775
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 1 cut(s) 866
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 1 cut(s) 534
BseRI GAGGAG 5 cut(s) 171, 324, 327, 579, 582
BseSI GKGCMC 1 cut(s) 805
BseXI GCAGC 4 cut(s) 192, 382, 461, 495
Bsh1236I CGCG 1 cut(s) 55
Bsh1285I CGRYCG 1 cut(s) 580
BshFI GGCC 2 cut(s) 332, 539
BsiEI CGRYCG 1 cut(s) 580
BsiHKAI GWGCWC 6 cut(s) 39, 120, 342, 510, 589, 805
BsiHKCI CYCGRG 1 cut(s) 22
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 1 cut(s) 866
BsmAI GTCTC 1 cut(s) 857
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 2 cut(s) 332, 539
Bso31I GGTCTC 1 cut(s) 857
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 6 cut(s) 39, 120, 342, 510, 589, 805
Bsp1407I TGTACA 1 cut(s) 786
Bsp143I GATC 5 cut(s) 67, 109, 265, 745, 890
BspACI CCGC 6 cut(s) 27, 53, 580, 596, 599, 602
BspANI GGCC 2 cut(s) 332, 539
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 331
BspPI GGATC 1 cut(s) 898
BspTI CTTAAG 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 857
BsrBI CCGCTC 1 cut(s) 27
BsrGI TGTACA 1 cut(s) 786
BsrI ACTGG 1 cut(s) 534
BssECI CCNNGG 1 cut(s) 765
BssMI GATC 5 cut(s) 67, 109, 265, 745, 890
BssSI CACGAG 1 cut(s) 833
BssT1I CCWWGG 1 cut(s) 765
Bst2BI CACGAG 1 cut(s) 833
Bst4CI ACNGT 1 cut(s) 713
BstAFI CTTAAG 1 cut(s) 461
BstAUI TGTACA 1 cut(s) 786
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 1 cut(s) 243
BstF5I GGATG 1 cut(s) 775
BstFNI CGCG 1 cut(s) 55
BstHHI GCGC 2 cut(s) 210, 678
BstKTI GATC 5 cut(s) 70, 112, 268, 748, 893
BstMAI GTCTC 1 cut(s) 857
BstMBI GATC 5 cut(s) 67, 109, 265, 745, 890
BstMCI CGRYCG 1 cut(s) 580
BstMWI GCNNNNNNNGC 2 cut(s) 214, 617
BstPAI GACNNNNGTC 1 cut(s) 387
BstSLI GKGCMC 1 cut(s) 805
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 4 cut(s) 192, 382, 461, 495
BsuRI GGCC 2 cut(s) 332, 539
BtsCI GGATG 1 cut(s) 775
Cac8I GCNNGC 1 cut(s) 368
CfoI GCGC 2 cut(s) 210, 678
Cfr13I GGNCC 1 cut(s) 330
CseI GACGC 2 cut(s) 44, 372
Csp6I GTAC 1 cut(s) 787
CviAII CATG 3 cut(s) 546, 556, 936
CviQI GTAC 1 cut(s) 787
DdeI CTNAG 1 cut(s) 243
DpnI GATC 5 cut(s) 69, 111, 267, 747, 892
DpnII GATC 5 cut(s) 67, 109, 265, 745, 890
DraI TTTAAA 1 cut(s) 736
EaeI YGGCCR 1 cut(s) 537
EciI GGCGGA 1 cut(s) 585
Ecl136II GAGCTC 2 cut(s) 508, 587
Eco130I CCWWGG 1 cut(s) 765
Eco24I GRGCYC 2 cut(s) 510, 589
Eco31I GGTCTC 1 cut(s) 857
Eco53kI GAGCTC 2 cut(s) 508, 587
Eco88I CYCGRG 1 cut(s) 22
EcoICRI GAGCTC 2 cut(s) 508, 587
EcoT14I CCWWGG 1 cut(s) 765
EcoT38I GRGCYC 2 cut(s) 510, 589
ErhI CCWWGG 1 cut(s) 765
FaeI CATG 3 cut(s) 549, 559, 939
FaqI GGGAC 1 cut(s) 375
FatI CATG 3 cut(s) 545, 555, 935
FauI CCCGC 1 cut(s) 60
FbaI TGATCA 2 cut(s) 109, 265
FblI GTMKAC 1 cut(s) 361
Fnu4HI GCNGC 6 cut(s) 206, 371, 450, 484, 599, 602
FokI GGATG 1 cut(s) 782
FriOI GRGCYC 2 cut(s) 510, 589
Fsp4HI GCNGC 6 cut(s) 206, 371, 450, 484, 599, 602
GlaI GCGC 2 cut(s) 209, 677
GluI GCNGC 6 cut(s) 206, 371, 450, 484, 599, 602
GsuI CTGGAG 1 cut(s) 607
HaeIII GGCC 2 cut(s) 332, 539
HgaI GACGC 2 cut(s) 44, 372
HhaI GCGC 2 cut(s) 210, 678
Hin1II CATG 3 cut(s) 549, 559, 939
Hin6I GCGC 2 cut(s) 208, 676
HinP1I GCGC 2 cut(s) 208, 676
HincII GTYRAC 1 cut(s) 362
HindII GTYRAC 1 cut(s) 362
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 9 cut(s) 250, 383, 400, 419, 497, 512, 533, 661, 826
Hpy166II GTNNAC 3 cut(s) 362, 787, 803
Hpy188I TCNGA 3 cut(s) 67, 577, 895
Hpy188III TCNNGA 6 cut(s) 71, 113, 269, 416, 818, 833
Hpy8I GTNNAC 3 cut(s) 362, 787, 803
Hpy99I CGWCG 3 cut(s) 60, 363, 366
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 1 cut(s) 713
HpyCH4IV ACGT 1 cut(s) 780
HpyCH4V TGCA 2 cut(s) 549, 803
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 617
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 780
Hsp92II CATG 3 cut(s) 549, 559, 939
HspAI GCGC 2 cut(s) 208, 676
Ksp22I TGATCA 2 cut(s) 109, 265
Kzo9I GATC 5 cut(s) 67, 109, 265, 745, 890
LmnI GCTCC 5 cut(s) 34, 158, 314, 592, 675
Lsp1109I GCAGC 4 cut(s) 192, 382, 461, 495
MaeII ACGT 1 cut(s) 780
MaeIII GTNAC 2 cut(s) 707, 713
MalI GATC 5 cut(s) 69, 111, 267, 747, 892
MbiI CCGCTC 1 cut(s) 27
MboI GATC 5 cut(s) 67, 109, 265, 745, 890
MboII GAAGA 1 cut(s) 894
MhlI GDGCHC 6 cut(s) 39, 120, 342, 510, 589, 805
MluCI AATT 4 cut(s) 184, 261, 718, 813
MlyI GAGTC 5 cut(s) 377, 413, 491, 506, 527
MmeI TCCRAC 2 cut(s) 133, 600
MroXI GAANNNNTTC 1 cut(s) 825
MseI TTAA 5 cut(s) 47, 225, 462, 735, 943
MslI CAYNNNNRTG 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 247
MspCI CTTAAG 1 cut(s) 461
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 214, 617
NdeII GATC 5 cut(s) 67, 109, 265, 745, 890
NlaIII CATG 3 cut(s) 549, 559, 939
NlaIV GGNNCC 1 cut(s) 331
NmeAIII GCCGAG 2 cut(s) 515, 608
PaeR7I CTCGAG 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PdmI GAANNNNTTC 1 cut(s) 825
PfeI GAWTC 4 cut(s) 250, 400, 661, 826
PflMI CCANNNNNTGG 1 cut(s) 866
PkrI GCNGC 6 cut(s) 207, 372, 451, 485, 600, 603
PleI GAGTC 5 cut(s) 377, 413, 491, 506, 527
PpsI GAGTC 5 cut(s) 377, 413, 491, 506, 527
PshAI GACNNNNGTC 1 cut(s) 387
Psp124BI GAGCTC 2 cut(s) 510, 589
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 1 cut(s) 330
PspXI VCTCGAGB 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 247
RsaI GTAC 1 cut(s) 788
RsaNI GTAC 1 cut(s) 787
RseI CAYNNNNRTG 1 cut(s) 560
SacI GAGCTC 2 cut(s) 510, 589
SalI GTCGAC 1 cut(s) 360
SaqAI TTAA 5 cut(s) 47, 225, 462, 735, 943
SatI GCNGC 6 cut(s) 206, 371, 450, 484, 599, 602
Sau3AI GATC 5 cut(s) 67, 109, 265, 745, 890
Sau96I GGNCC 1 cut(s) 330
SchI GAGTC 5 cut(s) 377, 413, 491, 506, 527
SduI GDGCHC 6 cut(s) 39, 120, 342, 510, 589, 805
Sfr274I CTCGAG 1 cut(s) 22
SgrDI CGTCGACG 1 cut(s) 360
SlaI CTCGAG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 560
SmlI CTYRAG 3 cut(s) 22, 414, 461
SmoI CTYRAG 3 cut(s) 22, 414, 461
Sse9I AATT 4 cut(s) 184, 261, 718, 813
SsiI CCGC 6 cut(s) 27, 53, 580, 596, 599, 602
SspI AATATT 1 cut(s) 925
SstI GAGCTC 2 cut(s) 510, 589
StyI CCWWGG 1 cut(s) 765
TaaI ACNGT 1 cut(s) 713
TaiI ACGT 1 cut(s) 783
TasI AATT 4 cut(s) 184, 261, 718, 813
TatI WGTACW 1 cut(s) 786
TauI GCSGC 2 cut(s) 601, 604
TfiI GAWTC 4 cut(s) 250, 400, 661, 826
Tru1I TTAA 5 cut(s) 47, 225, 462, 735, 943
Tru9I TTAA 5 cut(s) 47, 225, 462, 735, 943
TseI GCWGC 4 cut(s) 205, 370, 449, 483
TspDTI ATGAA 1 cut(s) 924
TspGWI ACGGA 2 cut(s) 161, 321
Van91I CCANNNNNTGG 1 cut(s) 866
Vha464I CTTAAG 1 cut(s) 461
VneI GTGCAC 1 cut(s) 801
XapI RAATTY 1 cut(s) 184
XhoI CTCGAG 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 361
XmnI GAANNNNTTC 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.