MD14G1129600.v1.1
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005955
Physical Location & Seq
Forward (+)
20743107 .. 20748432
5326 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1129600.v1.1.491

Sequence Viewer

Length: 1833 bp
ATGGAGAACCTGATATCATTGGTGAACAAAATCCAGAGAGCTTGCACTGCTCTAGGTGACCACGGCGAAGATAGCGCATTACCGACTCTTTGGGACTCTCTTCCCGCCATCGCCGTTGTCGGTGGCCAGAGTTCTGGCAAGTCTTCGGTTTTGGAGAGCATTGTTGGGAAGGATTTCTTACCACGCGGTTCTGGAATTGTTACTAGGCGTCCTCTTGTCTTGCAACTTATTAAGATTGACGAAGGAAGCAGAGAATATGCGGAGTTTCTCCACCTTCCAAGGAAGAGGTTCACTGATTTTGCTGCTGTGAGAAAGGAGATTGCAGACGAGACAGATCGAGAAACCGGCCTCAGCAAACAAATCTCTAGCGTTCCAATTCATCTTAGCATATATTCTCCTAATGTTGTTAACTTGACACTTGTTGACCTTCCCGGGCTTACAAAAGTGGCTGTTGAGGGTCAGTCGGATAATATTGTGCAAGACATTGAAAATATGGTTCGCTCCTTCATTGAGAAGCCCAACTGTATTATTCTAGCTATTTCACCTGCCAATCAAGATCTTGCCACATCTGATGCAATTAGGATCTCTCGCGAAGTGGATCCAACAGGGGACAGGACACTTGGAGTTTTGACAAAGATTGATCTAATGGACAAGGGTACGGATGCAGTTGAAATATTGGCAGGAAAGTCATACCGGCTTAAGTTTCCTTGGGTTGGGGTCGTGAATCGCTCACAAGCCGATATTAACAAGAATGTTGATATGATTGCTGCCAGGCTTAGAGAACGTGAGTATTTTTCTACCACCCCAGAATACAAGCACCTTGCTCCCAGAATGGGTTCTGAACATCTAGCTCGAATGCTTTCTAAGCATTTGGAAACTGTAATTAAGTCAAAAATTCCGGGCATCCAGTCCCTAATTAGCAAGACTGTTGCTGAACTTGAAACTGAACTGAGTCTTCTTGGAAAGCCTATTTCTGCAGATGCTGGAGGTAAACTGTATATAGTCATGGAGATTTGTCGTCTTTTTGATGGGATATATAAAGAACATCTTGATGGCTTGCGTTCAGGTGGTGATAAGATTTACAATATTTTTGATAATCAGCTTCCTGCTGCTCTGAAAAGATTGCAATTTGACAAGCAACTTTCAATGGAAAATATACGAAAGCTCATTACTGAAGCTGATGGATATCAACCTCATCTTATAGCTCCCGAACAAGGGTACCGTCGGCTCATCGAATCCTCTATAGTTAGTATGAGAGGTCCGGCTGAGGCAGCTGTTGATGCGGTTCATATCATTTTGAAGGATCTGGTTCGCAAGGCTATCAGTGAGACTCCAGAGCTAAAGCAGTATCCTGCTCTCAGAGTAGAGGTGACAAATGCAGCTACTGAGTCACTCGAGAGAATGAGAGAACAGAGCAAGAAGGCAACACTACAGCTAGTTGATATGGAATGTAGTTACCTGACGGCTGATTTCTTCCGTAATCTTCCCCAAGATGTTGAGAAGGGTGGAAACCCATCACATTCAATTTTTGACAGATACAACGATTCATACCTCAGGCGGATTGGGACCACGGTTTTGGCTTATGTCAATATGGTATGTGTAAGTTTGCGTAACTCAATTCCTAAGTCTGTGGTCTATTGTCAAGTCCGAGAGGCAAAACGAGTCTTACTTGACCAATTCTTCATCGAATTGGGAAAACTGGAGACAAAGCAGTTGTCGTCATTACTGAATGAGGACCCCGCAGTAATGGAGAGGCGTGCTGCCCTCGCAAGGAGGCTGGAGCTATACAGGAGTGCGCAAGCAGAAATTGATTCAGTTGCTTGGGCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000266 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000904 GO:0000910 GO:0000911 GO:0000919 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005856 GO:0005874 GO:0005886 GO:0005911 GO:0006810 GO:0006897 GO:0006898 GO:0006996 GO:0007005 GO:0007049 GO:0007275 GO:0007389 GO:0008017 GO:0008092 GO:0008150 GO:0009504 GO:0009506 GO:0009507 GO:0009524 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009832 GO:0009888 GO:0009920 GO:0009987 GO:0010015 GO:0010026 GO:0010051 GO:0010053 GO:0010054 GO:0010090 GO:0010091 GO:0010154 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022402 GO:0022414 GO:0022607 GO:0022622 GO:0030054 GO:0030154 GO:0030276 GO:0031090 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032989 GO:0034357 GO:0042546 GO:0042651 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043424 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044434 GO:0044435 GO:0044436 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051301 GO:0055035 GO:0055044 GO:0061458 GO:0061640 GO:0065007 GO:0071554 GO:0071669 GO:0071695 GO:0071840 GO:0071944 GO:0072583 GO:0090558 GO:0090626 GO:0090627 GO:0098588 GO:0098657 GO:0098805 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0140014 GO:1902410 GO:1903047 GO:1905392 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko04721 ko04961 ko05100 map04072 map04144 map04721 map04961 map05100
Pfam Domains
Protein Families
Dynamin_M Dynamin_N GED

Protein Analysis

611

Amino Acids

68.06

Weight (kDa)

6.83

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_N PF00350 37 - 213 7.1e-54 Dynamin family
Dynamin_M PF01031 221 - 488 4.7e-65 Dynamin central region
GED PF02212 514 - 606 4.2e-19 Dynamin GTPase effector domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 553
Acc16I TGCGCA 1 cut(s) 1797
Acc36I ACCTGC 1 cut(s) 553
Acc65I GGTACC 1 cut(s) 1218
AccB1I GGYRCC 1 cut(s) 1218
AccII CGCG 2 cut(s) 186, 591
AciI CCGC 6 cut(s) 105, 186, 260, 1283, 1558, 1740
AclWI GGATC 4 cut(s) 590, 593, 606, 1311
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 1 cut(s) 894
AcuI CTGAAG 1 cut(s) 1194
AcyI GRCGYC 1 cut(s) 208
AfaI GTAC 2 cut(s) 658, 1220
AfiI CCNNNNNNNGG 5 cut(s) 713, 833, 1214, 1215, 1770
AflII CTTAAG 1 cut(s) 698
AgsI TTSAA 6 cut(s) 488, 671, 941, 1146, 1300, 1524
AjnI CCWGG 1 cut(s) 770
Alw26I GTCTC 3 cut(s) 323, 1322, 1697
AlwI GGATC 4 cut(s) 590, 593, 606, 1311
AlwNI CAGNNNCTG 2 cut(s) 983, 1385
Ama87I CYCGRG 2 cut(s) 431, 1394
AoxI GGCC 2 cut(s) 124, 346
ApeKI GCWGC 6 cut(s) 302, 767, 1109, 1271, 1379, 1760
ApoI RAATTY 1 cut(s) 894
ArsI GACNNNNNNTTYG 4 cut(s) 1558, 1590, 1700, 1732
Asp700I GAANNNNTTC 4 cut(s) 173, 287, 835, 859
Asp718I GGTACC 1 cut(s) 1218
AspLEI GCGC 2 cut(s) 77, 1798
AspS9I GGNCC 3 cut(s) 1259, 1566, 1735
AsuC2I CCSGG 3 cut(s) 432, 433, 900
AsuHPI GGTGA 5 cut(s) 34, 68, 534, 1082, 1381
AvaI CYCGRG 2 cut(s) 431, 1394
AvaII GGWCC 3 cut(s) 1259, 1566, 1735
AxyI CCTNAGG 1 cut(s) 1553
BaeI ACNNNNGTAYC 2 cut(s) 1202, 1235
BalI TGGCCA 1 cut(s) 126
BamHI GGATCC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 1218
BbsI GAAGAC 2 cut(s) 135, 947
BbvCI CCTCAGC 2 cut(s) 350, 1266
BbvI GCAGC 6 cut(s) 289, 754, 1096, 1283, 1391, 1747
BccI CCATC 5 cut(s) 116, 1022, 1046, 1175, 1522
BceAI ACGGC 3 cut(s) 79, 98, 1479
BciT130I CCWGG 1 cut(s) 772
BciVI GTATCC 1 cut(s) 1359
BcnI CCSGG 3 cut(s) 432, 433, 900
BcoDI GTCTC 3 cut(s) 323, 1322, 1697
BfaI CTAG 6 cut(s) 53, 204, 366, 533, 848, 1436
BfmI CTRYAG 3 cut(s) 975, 1242, 1430
BfrI CTTAAG 1 cut(s) 698
BfuAI ACCTGC 1 cut(s) 553
BfuI GTATCC 1 cut(s) 1359
BglII AGATCT 1 cut(s) 556
BisI GCNGC 6 cut(s) 303, 768, 1110, 1272, 1380, 1761
BlsI GCNGC 6 cut(s) 304, 769, 1111, 1273, 1381, 1762
Bme1390I CCNGG 4 cut(s) 432, 433, 772, 900
Bme18I GGWCC 3 cut(s) 1259, 1566, 1735
BmeT110I CYCGRG 2 cut(s) 431, 1394
BmgT120I GGNCC 3 cut(s) 1259, 1566, 1735
BmiI GGNNCC 4 cut(s) 600, 1220, 1567, 1737
BmrFI CCNGG 4 cut(s) 432, 433, 772, 900
BmsI GCATC 5 cut(s) 562, 652, 912, 970, 1270
BpiI GAAGAC 2 cut(s) 135, 947
BpmI CTGGAG 4 cut(s) 1005, 1317, 1721, 1799
Bpu10I CCTNAGC 2 cut(s) 350, 1266
BpuMI CCSGG 3 cut(s) 432, 433, 900
BsaBI GATNNNNATC 1 cut(s) 1185
BsaHI GRCGYC 1 cut(s) 208
BsaJI CCNNGG 5 cut(s) 61, 278, 431, 707, 1569
Bsc4I CCNNNNNNNGG 5 cut(s) 713, 833, 1214, 1215, 1770
Bse118I RCCGGY 2 cut(s) 344, 693
Bse1I ACTGG 2 cut(s) 907, 1704
Bse21I CCTNAGG 1 cut(s) 1553
Bse8I GATNNNNATC 1 cut(s) 1185
BseBI CCWGG 1 cut(s) 772
BseDI CCNNGG 5 cut(s) 61, 278, 431, 707, 1569
BseGI GGATG 2 cut(s) 667, 903
BseJI GATNNNNATC 1 cut(s) 1185
BseLI CCNNNNNNNGG 5 cut(s) 713, 833, 1214, 1215, 1770
BseMII CTCAG 6 cut(s) 364, 941, 1257, 1372, 1377, 1567
BseNI ACTGG 2 cut(s) 907, 1704
BseXI GCAGC 6 cut(s) 289, 754, 1096, 1283, 1391, 1747
Bsh1236I CGCG 2 cut(s) 186, 591
BshFI GGCC 2 cut(s) 126, 348
BshNI GGYRCC 1 cut(s) 1218
BsiHKCI CYCGRG 2 cut(s) 431, 1394
BsiSI CCGG 5 cut(s) 345, 432, 694, 899, 1262
BslFI GGGAC 4 cut(s) 107, 623, 895, 1579
BslI CCNNNNNNNGG 5 cut(s) 713, 833, 1214, 1215, 1770
BsmAI GTCTC 3 cut(s) 323, 1322, 1697
BsmFI GGGAC 4 cut(s) 107, 623, 895, 1579
BsmI GAATGC 1 cut(s) 861
BsnI GGCC 2 cut(s) 126, 348
BsoBI CYCGRG 2 cut(s) 431, 1394
Bsp143I GATC 6 cut(s) 334, 556, 582, 598, 640, 1303
Bsp68I TCGCGA 1 cut(s) 591
BspACI CCGC 6 cut(s) 105, 186, 260, 1283, 1558, 1740
BspANI GGCC 2 cut(s) 126, 348
BspCNI CTCAG 6 cut(s) 363, 942, 1258, 1371, 1378, 1566
BspFNI CGCG 2 cut(s) 186, 591
BspLI GGNNCC 4 cut(s) 600, 1220, 1567, 1737
BspMAI CTGCAG 1 cut(s) 979
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 4 cut(s) 590, 593, 606, 1311
BspT107I GGYRCC 1 cut(s) 1218
BspTI CTTAAG 1 cut(s) 698
BsrFI RCCGGY 2 cut(s) 344, 693
BsrI ACTGG 2 cut(s) 907, 1704
BssAI RCCGGY 2 cut(s) 344, 693
BssECI CCNNGG 5 cut(s) 61, 278, 431, 707, 1569
BssMI GATC 6 cut(s) 334, 556, 582, 598, 640, 1303
BssNI GRCGYC 1 cut(s) 208
BssT1I CCWWGG 2 cut(s) 278, 707
Bst2UI CCWGG 1 cut(s) 772
Bst4CI ACNGT 6 cut(s) 524, 880, 928, 996, 1223, 1573
Bst6I CTCTTC 2 cut(s) 105, 278
BstACI GRCGYC 1 cut(s) 208
BstAFI CTTAAG 1 cut(s) 698
BstC8I GCNNGC 4 cut(s) 43, 1058, 1758, 1800
BstDSI CCRYGG 2 cut(s) 61, 1569
BstEII GGTNACC 1 cut(s) 56
BstF5I GGATG 2 cut(s) 667, 903
BstFNI CGCG 2 cut(s) 186, 591
BstHHI GCGC 2 cut(s) 77, 1798
BstKTI GATC 6 cut(s) 337, 559, 585, 601, 643, 1306
BstMAI GTCTC 3 cut(s) 323, 1322, 1697
BstMBI GATC 6 cut(s) 334, 556, 582, 598, 640, 1303
BstMWI GCNNNNNNNGC 6 cut(s) 47, 72, 865, 1271, 1280, 1766
BstNI CCWGG 1 cut(s) 772
BstPI GGTNACC 1 cut(s) 56
BstSCI CCNGG 4 cut(s) 430, 431, 770, 898
BstSFI CTRYAG 3 cut(s) 975, 1242, 1430
BstUI CGCG 2 cut(s) 186, 591
BstV1I GCAGC 6 cut(s) 289, 754, 1096, 1283, 1391, 1747
BstV2I GAAGAC 2 cut(s) 135, 947
BstX2I RGATCY 4 cut(s) 556, 582, 598, 1303
BstXI CCANNNNNNTGG 2 cut(s) 134, 1576
BstYI RGATCY 4 cut(s) 556, 582, 598, 1303
Bsu36I CCTNAGG 1 cut(s) 1553
BsuI GTATCC 1 cut(s) 1359
BsuRI GGCC 2 cut(s) 126, 348
BtgI CCRYGG 2 cut(s) 61, 1569
BtgZI GCGATG 1 cut(s) 94
BtsCI GGATG 2 cut(s) 667, 903
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 3 cut(s) 45, 291, 1330
BtuMI TCGCGA 1 cut(s) 591
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 4 cut(s) 43, 1058, 1758, 1800
CaiI CAGNNNCTG 2 cut(s) 983, 1385
CfoI GCGC 2 cut(s) 77, 1798
Cfr10I RCCGGY 2 cut(s) 344, 693
Cfr13I GGNCC 3 cut(s) 1259, 1566, 1735
Cfr9I CCCGGG 1 cut(s) 431
CseI GACGC 1 cut(s) 197
Csp6I GTAC 2 cut(s) 657, 1219
CviAII CATG 1 cut(s) 1006
CviQI GTAC 2 cut(s) 657, 1219
DpnI GATC 6 cut(s) 336, 558, 584, 600, 642, 1305
DpnII GATC 6 cut(s) 334, 556, 582, 598, 640, 1303
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 2 cut(s) 105, 278
EarI CTCTTC 2 cut(s) 105, 278
EciI GGCGGA 1 cut(s) 1573
Eco130I CCWWGG 2 cut(s) 278, 707
Eco32I GATATC 2 cut(s) 15, 1187
Eco47I GGWCC 3 cut(s) 1259, 1566, 1735
Eco57I CTGAAG 1 cut(s) 1194
Eco81I CCTNAGG 1 cut(s) 1553
Eco88I CYCGRG 2 cut(s) 431, 1394
Eco91I GGTNACC 1 cut(s) 56
EcoO109I RGGNCCY 1 cut(s) 1735
EcoO65I GGTNACC 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 770
EcoRV GATATC 2 cut(s) 15, 1187
EcoT14I CCWWGG 2 cut(s) 278, 707
ErhI CCWWGG 2 cut(s) 278, 707
FaeI CATG 1 cut(s) 1009
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 1032, 1064
FaqI GGGAC 4 cut(s) 107, 623, 895, 1579
FatI CATG 1 cut(s) 1005
FauI CCCGC 2 cut(s) 112, 1747
Fnu4HI GCNGC 6 cut(s) 303, 768, 1110, 1272, 1380, 1761
FokI GGATG 2 cut(s) 674, 890
Fsp4HI GCNGC 6 cut(s) 303, 768, 1110, 1272, 1380, 1761
FspBI CTAG 6 cut(s) 53, 204, 366, 533, 848, 1436
FspI TGCGCA 1 cut(s) 1797
GlaI GCGC 2 cut(s) 76, 1797
GluI GCNGC 6 cut(s) 303, 768, 1110, 1272, 1380, 1761
GsuI CTGGAG 4 cut(s) 1005, 1317, 1721, 1799
HaeIII GGCC 2 cut(s) 126, 348
HapII CCGG 5 cut(s) 345, 432, 694, 899, 1262
HgaI GACGC 1 cut(s) 197
HhaI GCGC 2 cut(s) 77, 1798
Hin1I GRCGYC 1 cut(s) 208
Hin1II CATG 1 cut(s) 1009
Hin6I GCGC 2 cut(s) 75, 1796
HinP1I GCGC 2 cut(s) 75, 1796
HincII GTYRAC 2 cut(s) 409, 424
HindII GTYRAC 2 cut(s) 409, 424
HpaI GTTAAC 1 cut(s) 409
HpaII CCGG 5 cut(s) 345, 432, 694, 899, 1262
HphI GGTGA 5 cut(s) 34, 68, 534, 1082, 1381
Hpy166II GTNNAC 5 cut(s) 25, 291, 409, 424, 992
Hpy188I TCNGA 6 cut(s) 466, 571, 841, 1116, 1361, 1649
Hpy8I GTNNAC 5 cut(s) 25, 291, 409, 424, 992
Hpy99I CGWCG 1 cut(s) 1227
HpyAV CCTTC 8 cut(s) 163, 236, 284, 437, 514, 1294, 1414, 1495
HpyCH4III ACNGT 6 cut(s) 524, 880, 928, 996, 1223, 1573
HpyCH4IV ACGT 1 cut(s) 784
HpyCH4V TGCA 9 cut(s) 45, 223, 323, 478, 575, 665, 977, 1126, 1379
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 72, 865, 1271, 1280, 1766
HpySE526I ACGT 1 cut(s) 784
Hsp92I GRCGYC 1 cut(s) 208
Hsp92II CATG 1 cut(s) 1009
HspAI GCGC 2 cut(s) 75, 1796
KpnI GGTACC 1 cut(s) 1222
KspAI GTTAAC 1 cut(s) 409
Kzo9I GATC 6 cut(s) 334, 556, 582, 598, 640, 1303
LmnI GCTCC 4 cut(s) 506, 829, 1210, 1780
Lsp1109I GCAGC 6 cut(s) 289, 754, 1096, 1283, 1391, 1747
LweI GCATC 5 cut(s) 562, 652, 912, 970, 1270
MaeI CTAG 6 cut(s) 53, 204, 366, 533, 848, 1436
MaeII ACGT 1 cut(s) 784
MaeIII GTNAC 6 cut(s) 56, 199, 1369, 1389, 1454, 1610
MalI GATC 6 cut(s) 336, 558, 584, 600, 642, 1305
MboI GATC 6 cut(s) 334, 556, 582, 598, 640, 1303
MboII GAAGA 8 cut(s) 80, 92, 135, 295, 947, 1465, 1475, 1672
MflI RGATCY 4 cut(s) 556, 582, 598, 1303
MlsI TGGCCA 1 cut(s) 126
MluNI TGGCCA 1 cut(s) 126
MlyI GAGTC 6 cut(s) 79, 89, 961, 1324, 1397, 1671
MmeI TCCRAC 2 cut(s) 444, 626
Mox20I TGGCCA 1 cut(s) 126
MroXI GAANNNNTTC 4 cut(s) 173, 287, 835, 859
MscI TGGCCA 1 cut(s) 126
MseI TTAA 5 cut(s) 231, 408, 699, 744, 885
MslI CAYNNNNRTG 1 cut(s) 1050
Msp20I TGGCCA 1 cut(s) 126
MspA1I CMGCKG 1 cut(s) 1274
MspCI CTTAAG 1 cut(s) 698
MspI CCGG 5 cut(s) 345, 432, 694, 899, 1262
MspR9I CCNGG 4 cut(s) 432, 433, 772, 900
Mva1269I GAATGC 1 cut(s) 861
MvaI CCWGG 1 cut(s) 772
MvnI CGCG 2 cut(s) 186, 591
MwoI GCNNNNNNNGC 6 cut(s) 47, 72, 865, 1271, 1280, 1766
NciI CCSGG 3 cut(s) 432, 433, 900
NdeII GATC 6 cut(s) 334, 556, 582, 598, 640, 1303
NlaIII CATG 1 cut(s) 1009
NlaIV GGNNCC 4 cut(s) 600, 1220, 1567, 1737
NmuCI GTSAC 3 cut(s) 56, 1369, 1389
NruI TCGCGA 1 cut(s) 591
NsbI TGCGCA 1 cut(s) 1797
PaeR7I CTCGAG 1 cut(s) 1394
PaqCI CACCTGC 1 cut(s) 553
PctI GAATGC 1 cut(s) 861
PdmI GAANNNNTTC 4 cut(s) 173, 287, 835, 859
PfeI GAWTC 4 cut(s) 724, 1235, 1544, 1811
PkrI GCNGC 6 cut(s) 304, 769, 1111, 1273, 1381, 1762
PleI GAGTC 6 cut(s) 79, 89, 960, 1324, 1396, 1670
PpsI GAGTC 6 cut(s) 79, 89, 960, 1324, 1396, 1670
PpuMI RGGWCCY 1 cut(s) 1735
Psp5II RGGWCCY 1 cut(s) 1735
Psp6I CCWGG 1 cut(s) 770
PspEI GGTNACC 1 cut(s) 56
PspGI CCWGG 1 cut(s) 770
PspN4I GGNNCC 4 cut(s) 600, 1220, 1567, 1737
PspPI GGNCC 3 cut(s) 1259, 1566, 1735
PspPPI RGGWCCY 1 cut(s) 1735
PstI CTGCAG 1 cut(s) 979
PstNI CAGNNNCTG 2 cut(s) 983, 1385
PsuI RGATCY 4 cut(s) 556, 582, 598, 1303
PvuII CAGCTG 1 cut(s) 1274
RruI TCGCGA 1 cut(s) 591
RsaI GTAC 2 cut(s) 658, 1220
RsaNI GTAC 2 cut(s) 657, 1219
RseI CAYNNNNRTG 1 cut(s) 1050
SaqAI TTAA 5 cut(s) 231, 408, 699, 744, 885
SatI GCNGC 6 cut(s) 303, 768, 1110, 1272, 1380, 1761
Sau3AI GATC 6 cut(s) 334, 556, 582, 598, 640, 1303
Sau96I GGNCC 3 cut(s) 1259, 1566, 1735
SchI GAGTC 6 cut(s) 79, 89, 961, 1324, 1397, 1671
ScrFI CCNGG 4 cut(s) 432, 433, 772, 900
SfaNI GCATC 5 cut(s) 562, 652, 912, 970, 1270
SfcI CTRYAG 3 cut(s) 975, 1242, 1430
Sfr274I CTCGAG 1 cut(s) 1394
SinI GGWCC 3 cut(s) 1259, 1566, 1735
SlaI CTCGAG 1 cut(s) 1394
SmaI CCCGGG 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 1050
SmlI CTYRAG 2 cut(s) 698, 1394
SmoI CTYRAG 2 cut(s) 698, 1394
SsiI CCGC 6 cut(s) 105, 186, 260, 1283, 1558, 1740
SspI AATATT 3 cut(s) 472, 675, 1087
SspMI CTAG 6 cut(s) 53, 204, 366, 533, 848, 1436
StyD4I CCNGG 4 cut(s) 430, 431, 770, 898
StyI CCWWGG 2 cut(s) 278, 707
TaaI ACNGT 6 cut(s) 524, 880, 928, 996, 1223, 1573
TaiI ACGT 1 cut(s) 787
TaqI TCGA 5 cut(s) 337, 853, 1233, 1395, 1686
TfiI GAWTC 4 cut(s) 724, 1235, 1544, 1811
Tru1I TTAA 5 cut(s) 231, 408, 699, 744, 885
Tru9I TTAA 5 cut(s) 231, 408, 699, 744, 885
TscAI CASTG 3 cut(s) 52, 298, 1330
TseFI GTSAC 3 cut(s) 56, 1369, 1389
TseI GCWGC 6 cut(s) 302, 767, 1109, 1271, 1379, 1760
Tsp45I GTSAC 3 cut(s) 56, 1369, 1389
TspDTI ATGAA 5 cut(s) 368, 496, 1277, 1536, 1672
TspGWI ACGGA 2 cut(s) 674, 1466
TspMI CCCGGG 1 cut(s) 431
TspRI CASTG 3 cut(s) 52, 298, 1330
Vha464I CTTAAG 1 cut(s) 698
VpaK11BI GGWCC 3 cut(s) 1259, 1566, 1735
XapI RAATTY 1 cut(s) 894
XhoI CTCGAG 1 cut(s) 1394
XmaI CCCGGG 1 cut(s) 431
XmnI GAANNNNTTC 4 cut(s) 173, 287, 835, 859
XspI CTAG 6 cut(s) 53, 204, 366, 533, 848, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.