MD14G1148000.v1.1

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006889
Physical Location & Seq
Forward (+)
24072454 .. 24087258
14805 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1148000.v1.1.491

Sequence Viewer

Length: 2073 bp
ATGAGCATCGATCAAAACCTTGCCGGAAATATATATACCAAACAGTTGCCAATTTTTGTAGTGGAAATACAATTTTTTGTCCATTCAGGTGAAGGCACATGCTGTAACAGCGCCAAAGGCAAAGAGACCATCCACAAATTAGTACGAACCAGAGAGAGAGAGAGACAGAGATACCGAGAGCACTACGACTGCGAGGCCGCCGCCGGCGCCGGACCTATCACTCCAGTTACAATACCCGAGACATCACCTTATCCCAGGGTTTTTGAAATGGGGAAAGAGAACCAGGCGTCAGCCATGGAAATCGACGATCCAAAGAACAACAGCTCCGATCAGATCTCCCCTAAATTCTCCATTAACGTGTTGCAGCTCTTGAAATCTGCTCAGATGCAGCACGGTTTGCGCCATGGAGATTACACTCGTTATCGGAGGTATTGCACTGCAAGGTTGAGGAGGTTGTACAAGTCGTTGAAGTTCACTCACGGCCGCGGTAAATACAACCGCCGGGCCATTACTGAATCTACTGTCACCGAAGTGAGGTATCTTCATATGGTTCTTTATACAGCAGAGAGAGCATGGAGCCATGCCATGGAAAAGAGACAGGTTCTGGATGGTCCAAATGCTCGTCAGCGGATCTATCTAATTGGTAGGCTGAGGAAGGCAGTAAAATGGGCTACTCTTTTCGCACAGTTGTGCGCAGTCAAGGGAGATTCCAGAACTTCATTAGAAGCTGAGGCTTATGCCTCCTTTATGAAAGGGAGCTTGTTGTTTGAGCAAGATCAGAATTGGGACACTGCATTGATGAATTTCAAAAGTGCCAGGGCTGTTTATGAGGAACTTGCGAAATATGGAGACCTGGAGAATCAAGTTTTGTGCCGTGAACGTGTTGAGGAATTAGAACCTAGTATCCGATACTGCCTACACAAGGTTGGAGAGTCAAATCTACAAGCCTCCAAACTTCTACAGATTGGGGAGATGGAAGGACCTGCGCTGGACCTCTTCAAGGCTAAATTGGAGGCTGTTATGGCCGAGGCAAGATCTCAACAGGCTGCCTCCATGACAGAGTTTCATTGGCTTGGTCATAGATTTCCAATTTCAAATCCAAAAACTCGAGTTTCCATTTTAAAAGCTCAAGAATTGGAGAAAGATTTGCTTGGTTCATCGACTAATTCACTCCTACCAGAGAAAAAAATAGGCATTTTTGACAAAATTTTTACCGCATACCATGAGGCAAGGGGTTGCATTCGCAATGACTTGGCTAGTGCAGGTAATTCTGAAAATGTGAAGGATGACTTGAATGGCCTCGATAAAGCTGTTAGTGCTGTATTGGGACAGCGAACCATTGAACGAAACCAACTGTTGGTTGCCATTGCGAAGAGTAAACTTACCAAAAGACGTGATGACAAAAACGAGAAAGCTACTAAGCCTGAAGAGCTTGTTCGGTTGTATGATCTACTTTTACAGAATACATCTGATCTATCAGATCTAGTCAGCTCTGGAAGAGACAGAAAACCAGAAGAAGTGACTTTTGCTGACGAATGTGAACTAAAAAGTTTGGCTTTCCGGGCAGAGAGGTGTTTCTTTCTAGGTAAATCATATAGCTTGGCCGGTAAGAGGCTAGAAGCTTATGTGTTGTACTGCCATGCTCGCTCCCTTGCAGAGAATGCCCTACAGAAATTTCAATCTGTGAATGACGGTGACGAGGCGATGATCAAAGAGTTGAAGACATTGTGTGATGAGTGCAGATCCAACAGTTGCATAGAACATGCCACGGGGATCATGGAAGAGCTTCAGGCTCCTGAAAACCTTTCAAAAAAGATATCTAATATCAACTTAACTGGTGTTGACAAGAAGGTGGAGTTCCTCCTCGAGAAACTTGATGTCTATGAATCTGCAGTTGGCGATGCAAACGTGAAAAGTGCTCCACGGATTGAAGCTTTCCCTCCTCCCTTTCAATCAATTCCTCGAAACCCTATTGTGCTCGACCTTGCCTACAATCATATTGACTTCCCGTCACTTCAACATAGGATGAAGAAAGACAAAAGCGGCGGCTTCCTTAAAAGGTTTTGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
SRP68

Protein Analysis

691

Amino Acids

78.32

Weight (kDa)

8.8

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP68 PF16969 133 - 667 3e-163 RNA-binding signal recognition particle 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 694
Acc36I ACCTGC 2 cut(s) 991, 1253
AccB1I GGYRCC 1 cut(s) 206
AccB7I CCANNNNNTGG 2 cut(s) 586, 1357
AccII CGCG 1 cut(s) 486
AciI CCGC 9 cut(s) 198, 201, 484, 486, 499, 628, 1215, 2043, 2046
AclWI GGATC 4 cut(s) 302, 638, 1737, 1781
AcoI YGGCCR 3 cut(s) 481, 1023, 1602
AcsI RAATTY 4 cut(s) 344, 802, 1206, 1673
AcuI CTGAAG 2 cut(s) 1446, 1772
AcyI GRCGYC 2 cut(s) 207, 287
AfaI GTAC 3 cut(s) 144, 458, 1634
AfiI CCNNNNNNNGG 6 cut(s) 534, 586, 922, 1000, 1357, 1611
AflIII ACRYGT 2 cut(s) 357, 880
AhdI GACNNNNNGTC 1 cut(s) 2008
AjiI CACGTC 1 cut(s) 1394
AjnI CCWGG 4 cut(s) 254, 282, 815, 852
AjuI GAANNNNNNNTTGG 2 cut(s) 1878, 1910
AleI CACNNNNGTG 2 cut(s) 530, 688
AloI GAACNNNNNNTCC 4 cut(s) 308, 340, 888, 920
Alw21I GWGCWC 3 cut(s) 183, 1921, 1980
Alw26I GTCTC 6 cut(s) 119, 157, 233, 589, 843, 1494
AlwI GGATC 4 cut(s) 302, 638, 1737, 1781
AlwNI CAGNNNCTG 1 cut(s) 604
Ama87I CYCGRG 3 cut(s) 236, 1107, 1865
AoxI GGCC 6 cut(s) 195, 481, 504, 1023, 1297, 1602
ApeKI GCWGC 3 cut(s) 364, 388, 1046
ApoI RAATTY 4 cut(s) 344, 802, 1206, 1673
Asp700I GAANNNNTTC 1 cut(s) 1785
AspLEI GCGC 5 cut(s) 113, 209, 402, 695, 988
AspS9I GGNCC 5 cut(s) 212, 504, 611, 980, 991
AsuC2I CCSGG 2 cut(s) 503, 1562
AsuHPI GGTGA 4 cut(s) 101, 237, 517, 1706
AvaI CYCGRG 3 cut(s) 236, 1107, 1865
AvaII GGWCC 4 cut(s) 212, 611, 980, 991
BanI GGYRCC 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 1727
Bbv12I GWGCWC 3 cut(s) 183, 1921, 1980
BbvCI CCTCAGC 2 cut(s) 650, 729
BbvI GCAGC 3 cut(s) 376, 400, 1033
BccI CCATC 3 cut(s) 137, 602, 967
BceAI ACGGC 2 cut(s) 496, 858
BciT130I CCWGG 4 cut(s) 256, 284, 817, 854
BciVI GTATCC 1 cut(s) 914
BclI TGATCA 1 cut(s) 1707
BcnI CCSGG 2 cut(s) 503, 1562
BcoDI GTCTC 6 cut(s) 119, 157, 233, 589, 843, 1494
BfaI CTAG 5 cut(s) 900, 1257, 1484, 1583, 1616
BfmI CTRYAG 3 cut(s) 959, 1667, 1890
BfoI RGCGCY 2 cut(s) 114, 210
BfuAI ACCTGC 2 cut(s) 991, 1253
BfuI GTATCC 1 cut(s) 914
BglII AGATCT 3 cut(s) 333, 1034, 1480
BisI GCNGC 8 cut(s) 198, 201, 365, 389, 484, 1047, 2044, 2047
BlsI GCNGC 8 cut(s) 199, 202, 366, 390, 485, 1048, 2045, 2048
Bme1390I CCNGG 6 cut(s) 256, 284, 503, 817, 854, 1562
Bme18I GGWCC 4 cut(s) 212, 611, 980, 991
BmeRI GACNNNNNGTC 1 cut(s) 2008
BmeT110I CYCGRG 3 cut(s) 236, 1107, 1865
BmgBI CACGTC 1 cut(s) 1394
BmgT120I GGNCC 5 cut(s) 212, 504, 611, 980, 991
BmiI GGNNCC 3 cut(s) 208, 578, 1794
BmrFI CCNGG 6 cut(s) 256, 284, 503, 817, 854, 1562
BmsI GCATC 3 cut(s) 15, 375, 1891
BpiI GAAGAC 1 cut(s) 1727
BpmI CTGGAG 2 cut(s) 207, 875
Bpu10I CCTNAGC 2 cut(s) 650, 729
BpuEI CTTGAG 1 cut(s) 1113
BpuMI CCSGG 2 cut(s) 503, 1562
Bsa29I ATCGAT 1 cut(s) 9
BsaHI GRCGYC 2 cut(s) 207, 287
BsaI GGTCTC 2 cut(s) 119, 843
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 6 cut(s) 534, 586, 922, 1000, 1357, 1611
Bse118I RCCGGY 2 cut(s) 203, 1604
Bse1I ACTGG 2 cut(s) 224, 1840
Bse3DI GCAATG 2 cut(s) 1252, 1365
BseBI CCWGG 4 cut(s) 256, 284, 817, 854
BseCI ATCGAT 1 cut(s) 9
BseGI GGATG 4 cut(s) 129, 613, 1291, 2031
BseLI CCNNNNNNNGG 6 cut(s) 534, 586, 922, 1000, 1357, 1611
BseMI GCAATG 2 cut(s) 1252, 1365
BseMII CTCAG 3 cut(s) 395, 641, 720
BseNI ACTGG 2 cut(s) 224, 1840
BseRI GAGGAG 3 cut(s) 463, 1853, 1932
BseX3I CGGCCG 1 cut(s) 481
BseXI GCAGC 3 cut(s) 376, 400, 1033
BsgI GTGCAG 2 cut(s) 1281, 1759
Bsh1236I CGCG 1 cut(s) 486
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 6 cut(s) 197, 483, 506, 1025, 1299, 1604
BshNI GGYRCC 1 cut(s) 206
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 484
BsiHKAI GWGCWC 3 cut(s) 183, 1921, 1980
BsiHKCI CYCGRG 3 cut(s) 236, 1107, 1865
BsiSI CCGG 6 cut(s) 24, 204, 210, 502, 1561, 1605
BslFI GGGAC 2 cut(s) 800, 1341
BslI CCNNNNNNNGG 6 cut(s) 534, 586, 922, 1000, 1357, 1611
BsmAI GTCTC 6 cut(s) 119, 157, 233, 589, 843, 1494
BsmFI GGGAC 2 cut(s) 800, 1341
BsmI GAATGC 2 cut(s) 1239, 1666
BsnI GGCC 6 cut(s) 197, 483, 506, 1025, 1299, 1604
Bso31I GGTCTC 2 cut(s) 119, 843
BsoBI CYCGRG 3 cut(s) 236, 1107, 1865
Bsp1286I GDGCHC 3 cut(s) 183, 1921, 1980
Bsp1407I TGTACA 1 cut(s) 456
Bsp19I CCATGG 3 cut(s) 294, 403, 585
BspACI CCGC 9 cut(s) 198, 201, 484, 486, 499, 628, 1215, 2043, 2046
BspANI GGCC 6 cut(s) 197, 483, 506, 1025, 1299, 1604
BspCNI CTCAG 3 cut(s) 394, 642, 721
BspDI ATCGAT 1 cut(s) 9
BspFNI CGCG 1 cut(s) 486
BspLI GGNNCC 3 cut(s) 208, 578, 1794
BspMAI CTGCAG 1 cut(s) 1894
BspMI ACCTGC 2 cut(s) 991, 1253
BspPI GGATC 4 cut(s) 302, 638, 1737, 1781
BspQI GCTCTTC 2 cut(s) 1422, 1776
BspT107I GGYRCC 1 cut(s) 206
BspTNI GGTCTC 2 cut(s) 119, 843
BsrDI GCAATG 2 cut(s) 1252, 1365
BsrFI RCCGGY 2 cut(s) 203, 1604
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 2 cut(s) 224, 1840
BssAI RCCGGY 2 cut(s) 203, 1604
BssNI GRCGYC 2 cut(s) 207, 287
BssT1I CCWWGG 3 cut(s) 294, 403, 585
Bst2UI CCWGG 4 cut(s) 256, 284, 817, 854
Bst4CI ACNGT 7 cut(s) 45, 395, 523, 687, 1356, 1694, 1751
Bst6I CTCTTC 5 cut(s) 1001, 1367, 1422, 1492, 1776
BstACI GRCGYC 2 cut(s) 207, 287
BstAPI GCANNNNNTGC 2 cut(s) 397, 1661
BstAUI TGTACA 1 cut(s) 456
BstC8I GCNNGC 2 cut(s) 205, 1645
BstDEI CTNAG 4 cut(s) 381, 650, 729, 1419
BstDSI CCRYGG 6 cut(s) 294, 403, 484, 585, 1767, 1922
BstENI CCTNNNNNAGG 2 cut(s) 920, 998
BstF5I GGATG 4 cut(s) 129, 613, 1291, 2031
BstFNI CGCG 1 cut(s) 486
BstH2I RGCGCY 2 cut(s) 114, 210
BstHHI GCGC 5 cut(s) 113, 209, 402, 695, 988
BstMAI GTCTC 6 cut(s) 119, 157, 233, 589, 843, 1494
BstMCI CGRYCG 1 cut(s) 484
BstNI CCWGG 4 cut(s) 256, 284, 817, 854
BstNSI RCATGY 2 cut(s) 102, 1766
BstSCI CCNGG 6 cut(s) 254, 282, 501, 815, 852, 1560
BstSFI CTRYAG 3 cut(s) 959, 1667, 1890
BstUI CGCG 1 cut(s) 486
BstV1I GCAGC 3 cut(s) 376, 400, 1033
BstV2I GAAGAC 1 cut(s) 1727
BstX2I RGATCY 5 cut(s) 333, 630, 1034, 1480, 1742
BstYI RGATCY 5 cut(s) 333, 630, 1034, 1480, 1742
BstZI CGGCCG 1 cut(s) 481
Bsu15I ATCGAT 1 cut(s) 9
BsuI GTATCC 1 cut(s) 914
BsuRI GGCC 6 cut(s) 197, 483, 506, 1025, 1299, 1604
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 6 cut(s) 294, 403, 484, 585, 1767, 1922
BtgZI GCGATG 2 cut(s) 1718, 1914
BtrI CACGTC 1 cut(s) 1394
BtsCI GGATG 4 cut(s) 129, 613, 1291, 2031
BtsI GCAGTG 2 cut(s) 435, 789
BtsIMutI CAGTG 2 cut(s) 435, 789
BveI ACCTGC 2 cut(s) 991, 1253
Cac8I GCNNGC 2 cut(s) 205, 1645
CaiI CAGNNNCTG 1 cut(s) 604
CfoI GCGC 5 cut(s) 113, 209, 402, 695, 988
Cfr10I RCCGGY 2 cut(s) 203, 1604
Cfr13I GGNCC 5 cut(s) 212, 504, 611, 980, 991
Cfr42I CCGCGG 1 cut(s) 487
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 1 cut(s) 276
Csp6I GTAC 3 cut(s) 143, 457, 1633
CviQI GTAC 3 cut(s) 143, 457, 1633
DdeI CTNAG 4 cut(s) 381, 650, 729, 1419
DinI GGCGCC 1 cut(s) 208
DraI TTTAAA 1 cut(s) 1122
DriI GACNNNNNGTC 1 cut(s) 2008
EaeI YGGCCR 3 cut(s) 481, 1023, 1602
EagI CGGCCG 1 cut(s) 481
Eam1104I CTCTTC 5 cut(s) 1001, 1367, 1422, 1492, 1776
Eam1105I GACNNNNNGTC 1 cut(s) 2008
EarI CTCTTC 5 cut(s) 1001, 1367, 1422, 1492, 1776
EclXI CGGCCG 1 cut(s) 481
Eco130I CCWWGG 3 cut(s) 294, 403, 585
Eco31I GGTCTC 2 cut(s) 119, 843
Eco32I GATATC 1 cut(s) 1818
Eco47I GGWCC 4 cut(s) 212, 611, 980, 991
Eco52I CGGCCG 1 cut(s) 481
Eco57I CTGAAG 2 cut(s) 1446, 1772
Eco88I CYCGRG 3 cut(s) 236, 1107, 1865
EcoNI CCTNNNNNAGG 2 cut(s) 920, 998
EcoO109I RGGNCCY 1 cut(s) 980
EcoRII CCWGG 4 cut(s) 254, 282, 815, 852
EcoRV GATATC 1 cut(s) 1818
EcoT14I CCWWGG 3 cut(s) 294, 403, 585
EgeI GGCGCC 1 cut(s) 208
EheI GGCGCC 1 cut(s) 208
ErhI CCWWGG 3 cut(s) 294, 403, 585
FalI AAGNNNNNCTT 6 cut(s) 1134, 1166, 1274, 1306, 1540, 1572
FaqI GGGAC 2 cut(s) 800, 1341
FauNDI CATATG 1 cut(s) 546
FbaI TGATCA 1 cut(s) 1707
Fnu4HI GCNGC 8 cut(s) 198, 201, 365, 389, 484, 1047, 2044, 2047
FokI GGATG 4 cut(s) 116, 620, 1298, 2038
Fsp4HI GCNGC 8 cut(s) 198, 201, 365, 389, 484, 1047, 2044, 2047
FspBI CTAG 5 cut(s) 900, 1257, 1484, 1583, 1616
FspI TGCGCA 1 cut(s) 694
GlaI GCGC 5 cut(s) 112, 208, 401, 694, 987
GluI GCNGC 8 cut(s) 198, 201, 365, 389, 484, 1047, 2044, 2047
GsuI CTGGAG 2 cut(s) 207, 875
HaeII RGCGCY 2 cut(s) 114, 210
HaeIII GGCC 6 cut(s) 197, 483, 506, 1025, 1299, 1604
HapII CCGG 6 cut(s) 24, 204, 210, 502, 1561, 1605
HgaI GACGC 1 cut(s) 276
HhaI GCGC 5 cut(s) 113, 209, 402, 695, 988
Hin1I GRCGYC 2 cut(s) 207, 287
Hin6I GCGC 5 cut(s) 111, 207, 400, 693, 986
HinP1I GCGC 5 cut(s) 111, 207, 400, 693, 986
HincII GTYRAC 1 cut(s) 1843
HindII GTYRAC 1 cut(s) 1843
HindIII AAGCTT 2 cut(s) 1620, 1932
HinfI GANTC 5 cut(s) 515, 707, 859, 932, 1886
HpaII CCGG 6 cut(s) 24, 204, 210, 502, 1561, 1605
HphI GGTGA 4 cut(s) 101, 237, 517, 1706
Hpy166II GTNNAC 5 cut(s) 474, 878, 1379, 1541, 1843
Hpy188I TCNGA 9 cut(s) 328, 333, 384, 426, 780, 908, 1273, 1471, 1480
Hpy188III TCNNGA 7 cut(s) 370, 605, 711, 1130, 1494, 1796, 1867
Hpy8I GTNNAC 5 cut(s) 474, 878, 1379, 1541, 1843
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 5 cut(s) 86, 649, 971, 1276, 1843
HpyCH4III ACNGT 7 cut(s) 45, 395, 523, 687, 1356, 1694, 1751
HpyCH4IV ACGT 4 cut(s) 357, 880, 1393, 1908
HpyF3I CTNAG 4 cut(s) 381, 650, 729, 1419
HpySE526I ACGT 4 cut(s) 357, 880, 1393, 1908
Hsp92I GRCGYC 2 cut(s) 207, 287
HspAI GCGC 5 cut(s) 111, 207, 400, 693, 986
KasI GGCGCC 1 cut(s) 206
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 1707
KspI CCGCGG 1 cut(s) 487
LguI GCTCTTC 2 cut(s) 1422, 1776
LmnI GCTCC 6 cut(s) 329, 576, 756, 1652, 1798, 1924
Lsp1109I GCAGC 3 cut(s) 376, 400, 1033
LweI GCATC 3 cut(s) 15, 375, 1891
MaeI CTAG 5 cut(s) 900, 1257, 1484, 1583, 1616
MaeII ACGT 4 cut(s) 357, 880, 1393, 1908
MaeIII GTNAC 6 cut(s) 104, 226, 523, 1519, 1694, 2010
MboII GAAGA 9 cut(s) 533, 988, 1384, 1439, 1509, 1526, 1732, 1793, 2041
MflI RGATCY 5 cut(s) 333, 630, 1034, 1480, 1742
MhlI GDGCHC 3 cut(s) 183, 1921, 1980
Mly113I GGCGCC 1 cut(s) 207
MlyI GAGTC 1 cut(s) 941
MmeI TCCRAC 2 cut(s) 907, 1770
MreI CGCCGGCG 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 1785
MseI TTAA 4 cut(s) 354, 1121, 1832, 2055
MslI CAYNNNNRTG 4 cut(s) 87, 356, 530, 688
MspA1I CMGCKG 2 cut(s) 486, 628
MspI CCGG 6 cut(s) 24, 204, 210, 502, 1561, 1605
MspR9I CCNGG 6 cut(s) 256, 284, 503, 817, 854, 1562
Mva1269I GAATGC 2 cut(s) 1239, 1666
MvaI CCWGG 4 cut(s) 256, 284, 817, 854
MvnI CGCG 1 cut(s) 486
NaeI GCCGGC 1 cut(s) 205
NarI GGCGCC 1 cut(s) 207
NciI CCSGG 2 cut(s) 503, 1562
NcoI CCATGG 3 cut(s) 294, 403, 585
NdeI CATATG 1 cut(s) 546
NgoMIV GCCGGC 1 cut(s) 203
NlaIV GGNNCC 3 cut(s) 208, 578, 1794
NmeAIII GCCGAG 1 cut(s) 1051
NmuCI GTSAC 4 cut(s) 523, 1519, 1694, 2010
NsbI TGCGCA 1 cut(s) 694
NspI RCATGY 2 cut(s) 102, 1766
OliI CACNNNNGTG 2 cut(s) 530, 688
PaeR7I CTCGAG 2 cut(s) 1107, 1865
PasI CCCWGGG 1 cut(s) 255
PciSI GCTCTTC 2 cut(s) 1422, 1776
PctI GAATGC 2 cut(s) 1239, 1666
PdiI GCCGGC 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 1785
PfeI GAWTC 4 cut(s) 515, 707, 859, 1886
PflMI CCANNNNNTGG 2 cut(s) 586, 1357
PkrI GCNGC 8 cut(s) 199, 202, 366, 390, 485, 1048, 2045, 2048
PleI GAGTC 1 cut(s) 940
PluTI GGCGCC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 940
PpuMI RGGWCCY 1 cut(s) 980
Psp5II RGGWCCY 1 cut(s) 980
Psp6I CCWGG 4 cut(s) 254, 282, 815, 852
PspGI CCWGG 4 cut(s) 254, 282, 815, 852
PspN4I GGNNCC 3 cut(s) 208, 578, 1794
PspPI GGNCC 5 cut(s) 212, 504, 611, 980, 991
PspPPI RGGWCCY 1 cut(s) 980
PspXI VCTCGAGB 1 cut(s) 1107
PstI CTGCAG 1 cut(s) 1894
PstNI CAGNNNCTG 1 cut(s) 604
PsuI RGATCY 5 cut(s) 333, 630, 1034, 1480, 1742
RsaI GTAC 3 cut(s) 144, 458, 1634
RsaNI GTAC 3 cut(s) 143, 457, 1633
RseI CAYNNNNRTG 4 cut(s) 87, 356, 530, 688
SacII CCGCGG 1 cut(s) 487
SapI GCTCTTC 2 cut(s) 1422, 1776
SaqAI TTAA 4 cut(s) 354, 1121, 1832, 2055
SatI GCNGC 8 cut(s) 198, 201, 365, 389, 484, 1047, 2044, 2047
Sau96I GGNCC 5 cut(s) 212, 504, 611, 980, 991
SchI GAGTC 1 cut(s) 941
ScrFI CCNGG 6 cut(s) 256, 284, 503, 817, 854, 1562
SduI GDGCHC 3 cut(s) 183, 1921, 1980
SfaNI GCATC 3 cut(s) 15, 375, 1891
SfcI CTRYAG 3 cut(s) 959, 1667, 1890
SfoI GGCGCC 1 cut(s) 208
Sfr274I CTCGAG 2 cut(s) 1107, 1865
Sfr303I CCGCGG 1 cut(s) 487
SgrAI CRCCGGYG 1 cut(s) 203
SgrBI CCGCGG 1 cut(s) 487
SinI GGWCC 4 cut(s) 212, 611, 980, 991
SlaI CTCGAG 2 cut(s) 1107, 1865
SmiMI CAYNNNNRTG 4 cut(s) 87, 356, 530, 688
SmlI CTYRAG 3 cut(s) 1107, 1128, 1865
SmoI CTYRAG 3 cut(s) 1107, 1128, 1865
SsiI CCGC 9 cut(s) 198, 201, 484, 486, 499, 628, 1215, 2043, 2046
SspDI GGCGCC 1 cut(s) 206
SspMI CTAG 5 cut(s) 900, 1257, 1484, 1583, 1616
StyD4I CCNGG 6 cut(s) 254, 282, 501, 815, 852, 1560
StyI CCWWGG 3 cut(s) 294, 403, 585
TaaI ACNGT 7 cut(s) 45, 395, 523, 687, 1356, 1694, 1751
TaiI ACGT 4 cut(s) 360, 883, 1396, 1911
TaqI TCGA 8 cut(s) 9, 303, 1108, 1160, 1302, 1866, 1963, 1980
TatI WGTACW 2 cut(s) 456, 1632
TauI GCSGC 5 cut(s) 200, 203, 486, 2046, 2049
TfiI GAWTC 4 cut(s) 515, 707, 859, 1886
Tru1I TTAA 4 cut(s) 354, 1121, 1832, 2055
Tru9I TTAA 4 cut(s) 354, 1121, 1832, 2055
TscAI CASTG 2 cut(s) 442, 796
TseFI GTSAC 4 cut(s) 523, 1519, 1694, 2010
TseI GCWGC 3 cut(s) 364, 388, 1046
Tsp45I GTSAC 4 cut(s) 523, 1519, 1694, 2010
TspDTI ATGAA 8 cut(s) 533, 708, 764, 815, 1055, 1146, 1899, 2042
TspGWI ACGGA 1 cut(s) 1939
TspRI CASTG 2 cut(s) 442, 796
Van91I CCANNNNNTGG 2 cut(s) 586, 1357
VpaK11BI GGWCC 4 cut(s) 212, 611, 980, 991
XagI CCTNNNNNAGG 2 cut(s) 920, 998
XapI RAATTY 4 cut(s) 344, 802, 1206, 1673
XceI RCATGY 2 cut(s) 102, 1766
XcmI CCANNNNNNNNNTGG 1 cut(s) 1774
XhoI CTCGAG 2 cut(s) 1107, 1865
XmnI GAANNNNTTC 1 cut(s) 1785
XspI CTAG 5 cut(s) 900, 1257, 1484, 1583, 1616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.