Rroxscaffold_3G00266030

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006889
Physical Location & Seq
Reverse (-)
59407019 .. 59412324
5306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266030.1

Sequence Viewer

Length: 1812 bp
ATGGGGAAAGAGAACCAGAACCAGACGACGTCGGCTATGGAGATCGACAATCAAAAGGCGAACACCTCCGATCAGATCGGTCCTAAATTCTCAATCAACGTTTTACAGCTGTTGAAATCTGCTCAGATGCAGCATGGATTGCGCCATGGGGATTACACTCGTTATCGGAGGTATTGCACTGCAAGGTTGAGGAGGCTGTATAAGTCATTGAAGTTCACTCACGGCCGTGGCAAGTACAACCGCCGAGCAATAACCGAATCAACTGTTACTGAAGTGAGGTTTCTCCACGTGGTTCTATATACGGCAGAGAGGGCTTGGAGCCATGCCATGGAAAAGAGGCAAGTTCTGGATGGTCCAAATGCTCGTCAACGAATCTATCTGATTGGTAGGCTGAGGAAGGCAGTAAAATGGGCTACTCTTTTTGCACAGTTGTCTGCAATCAAGGGAGATTCTAGAACTTCCTTAGAAGCTGAGGCTTATGCCTCTTTTATGAAAGGGAACCTGTTGTTTGAACAAGATCGAAATTGGGACACAGCATTACTGAACTTCAAAAGTGCCAGGGCGGTTTATGAGGAACTTGCGAAATACGGAGACCTGGAGAATCAAGTTTTGTGCCGTGAGCGTGTTGAGGAATTAGAACCTAGTATCCGGTACTGTCTACACAAGATTGGAGAGTCAAATCTACAAGGCTCTGAACTTCTACATATTGGCGAGATGGAAGGACCTGCTTTGGACCTCTTCAAGGCTAAATTGGAGGCTGTTATGGATGAGGCAAGATCTCAACAGGCTGCCTCCATGACAGAATTTCATTGGCTAGGCCATAGATTTCCAATTTCGAATGCAAAAACTCGGGTTTCTATTTTAAAAGCTCAAGACTTGGAGAAAGATGTACTTGGTTCATCAGCTAATTCACTCCCTACAGAGAAAAGGCTAGCCATTTTTGACAAAATTTTCGCCGCATACCATGAGGCAAGGAGCTGCATTCGCAGTGATTTGGTCAGTGCAGGTAGTTCTGAAAATGTCAAAGATGACTTGAATGGCCTTGATAAAGCTGTTAGTGCTGTATTAGGACAACGGACCATTGAGCGTAACCTACTTTTGGTTAGCATTGCAAAGAGCAAACTCACTAAGCGACGTGATGACAAAAATGAGAGAACTACGAAGCCAGAAGAGCTTGTCCGCTTGTATGATCTCCTATTACAGAATACAGCTGACCTTTCAGACTTAGTCAGTTCGGGACGAGAAAAAACATCAGAGGAAGTCGCATTTGCTGCAGAATGTGAGCTTAAAAGTTTGGCTTTCCGAGCAGAAAGGTGTTTCTTTTTGGGTAGATCATACAGTTTGGCTGGTAAGAGGGTGGAAGCATATGCATTGTATTGCCATGCTCGCACCATTGCTGAGGATGCCCTGCAAAAATTTAAAGCTGTTGATAATGCTGATCAGGTGGTGATCAAAGAGTTGAAGCTATTGTGCGATGAATGCAGATCTAACAGTTGTATAGAGCATGCAACAGGAATCATGGAGGAGTTGAAAGCTCCTGAAAATCTCTCACAACAGATTTCTGACATCCATATATCTGGAGTTGACACGAAGGAGAAATTCCTCCTTGAGAAACTTGAGGTCTACGAATCTGCAGTTGGCGATTCAAATGTGAGAAGTGCTGCGCGAATTGAAGCTTTCCCTCCTCCCTTCCAAACAATTCCTCGCAATCCAATTGTTCTAGACCTTGCCTATGATCATATTGAGTTCCCATCTCTTCTAAATAGGATGAAGAAAGAAACAAAGGGCTTCATTAGTAGGTTTTGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
SRP68

Protein Analysis

603

Amino Acids

68.53

Weight (kDa)

8.76

Isoelectric Point (pI)

41.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP68 PF16969 47 - 581 4.6e-159 RNA-binding signal recognition particle 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 32
Acc36I ACCTGC 2 cut(s) 733, 995
AccB7I CCANNNNNTGG 1 cut(s) 328
AccI GTMKAC 2 cut(s) 658, 1623
AccII CGCG 1 cut(s) 1666
AciI CCGC 4 cut(s) 241, 563, 957, 1180
AclI AACGTT 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 5 cut(s) 86, 803, 948, 1415, 1598
AcuI CTGAAG 1 cut(s) 291
AcvI CACGTG 1 cut(s) 289
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 3 cut(s) 236, 653, 891
AfiI CCNNNNNNNGG 3 cut(s) 328, 742, 1099
AjiI CACGTC 1 cut(s) 1136
AjnI CCWGG 2 cut(s) 557, 594
AjuI GAANNNNNNNTTGG 2 cut(s) 1620, 1652
AleI CACNNNNGTG 1 cut(s) 225
AloI GAACNNNNNNTCC 2 cut(s) 630, 662
Alw26I GTCTC 1 cut(s) 585
Ama87I CYCGRG 1 cut(s) 849
AoxI GGCC 3 cut(s) 223, 817, 1039
ApeKI GCWGC 5 cut(s) 130, 788, 978, 1271, 1661
ApoI RAATTY 5 cut(s) 86, 803, 948, 1415, 1598
ArsI GACNNNNNNTTYG 2 cut(s) 935, 967
AspLEI GCGC 2 cut(s) 144, 1666
AspS9I GGNCC 5 cut(s) 80, 353, 722, 733, 1077
AsuHPI GGTGA 1 cut(s) 1459
AsuII TTCGAA 1 cut(s) 836
AsuNHI GCTAGC 1 cut(s) 931
AvaI CYCGRG 1 cut(s) 849
AvaII GGWCC 5 cut(s) 80, 353, 722, 733, 1077
BbrPI CACGTG 1 cut(s) 289
BbvCI CCTCAGC 3 cut(s) 392, 471, 1398
BbvI GCAGC 5 cut(s) 142, 775, 965, 1258, 1648
BccI CCATC 3 cut(s) 344, 709, 1759
BceAI ACGGC 4 cut(s) 210, 238, 318, 600
BciT130I CCWGG 2 cut(s) 559, 596
BciVI GTATCC 1 cut(s) 656
BclI TGATCA 3 cut(s) 1438, 1449, 1735
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 5 cut(s) 453, 642, 815, 932, 1721
BfmI CTRYAG 3 cut(s) 918, 1272, 1632
BfuAI ACCTGC 2 cut(s) 733, 995
BfuI GTATCC 1 cut(s) 656
BglII AGATCT 2 cut(s) 776, 1484
BisI GCNGC 6 cut(s) 131, 789, 957, 979, 1272, 1662
BlsI GCNGC 6 cut(s) 132, 790, 958, 980, 1273, 1663
Bme1390I CCNGG 2 cut(s) 559, 596
Bme18I GGWCC 5 cut(s) 80, 353, 722, 733, 1077
BmeT110I CYCGRG 1 cut(s) 849
BmgBI CACGTC 1 cut(s) 1136
BmgT120I GGNCC 5 cut(s) 80, 353, 722, 733, 1077
BmiI GGNNCC 2 cut(s) 320, 500
BmrFI CCNGG 2 cut(s) 559, 596
BmsI GCATC 2 cut(s) 117, 1393
BmtI GCTAGC 1 cut(s) 935
BpmI CTGGAG 2 cut(s) 617, 1599
Bpu10I CCTNAGC 3 cut(s) 392, 471, 1398
Bpu14I TTCGAA 1 cut(s) 836
BpuEI CTTGAG 3 cut(s) 855, 1628, 1637
BsaAI YACGTR 1 cut(s) 289
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 585
BsaJI CCNNGG 4 cut(s) 145, 226, 327, 558
BsaWI WCCGGW 1 cut(s) 648
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 742, 1099
Bse3DI GCAATG 2 cut(s) 1107, 1392
BseBI CCWGG 2 cut(s) 559, 596
BseDI CCNNGG 4 cut(s) 145, 226, 327, 558
BseGI GGATG 5 cut(s) 355, 772, 1408, 1566, 1773
BseLI CCNNNNNNNGG 3 cut(s) 328, 742, 1099
BseMI GCAATG 2 cut(s) 1107, 1392
BseMII CTCAG 4 cut(s) 137, 383, 462, 1389
BseRI GAGGAG 3 cut(s) 205, 1538, 1674
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 5 cut(s) 142, 775, 965, 1258, 1648
BsgI GTGCAG 1 cut(s) 1023
Bsh1236I CGCG 1 cut(s) 1666
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 3 cut(s) 225, 819, 1041
BsiEI CGRYCG 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 849
BsiSI CCGG 1 cut(s) 649
BslFI GGGAC 2 cut(s) 542, 1251
BslI CCNNNNNNNGG 3 cut(s) 328, 742, 1099
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 2 cut(s) 542, 1251
BsmI GAATGC 3 cut(s) 844, 981, 1484
BsnI GGCC 3 cut(s) 225, 819, 1041
Bso31I GGTCTC 1 cut(s) 585
BsoBI CYCGRG 1 cut(s) 849
Bsp119I TTCGAA 1 cut(s) 836
Bsp19I CCATGG 2 cut(s) 145, 327
BspACI CCGC 4 cut(s) 241, 563, 957, 1180
BspANI GGCC 3 cut(s) 225, 819, 1041
BspCNI CTCAG 4 cut(s) 136, 384, 463, 1390
BspFNI CGCG 1 cut(s) 1666
BspLI GGNNCC 2 cut(s) 320, 500
BspMAI CTGCAG 2 cut(s) 1276, 1636
BspMI ACCTGC 2 cut(s) 733, 995
BspOI GCTAGC 1 cut(s) 935
BspQI GCTCTTC 1 cut(s) 1164
BspT104I TTCGAA 1 cut(s) 836
BspTNI GGTCTC 1 cut(s) 585
BsrDI GCAATG 2 cut(s) 1107, 1392
BssECI CCNNGG 4 cut(s) 145, 226, 327, 558
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 2 cut(s) 145, 327
Bst2UI CCWGG 2 cut(s) 559, 596
Bst4CI ACNGT 5 cut(s) 265, 429, 656, 1340, 1493
Bst6I CTCTTC 3 cut(s) 743, 1164, 1761
BstACI GRCGYC 1 cut(s) 29
BstAPI GCANNNNNTGC 2 cut(s) 139, 1271
BstBAI YACGTR 1 cut(s) 289
BstBI TTCGAA 1 cut(s) 836
BstC8I GCNNGC 3 cut(s) 933, 1387, 1506
BstDEI CTNAG 7 cut(s) 123, 392, 463, 471, 1128, 1225, 1398
BstDSI CCRYGG 3 cut(s) 145, 226, 327
BstENI CCTNNNNNAGG 1 cut(s) 740
BstF5I GGATG 5 cut(s) 355, 772, 1408, 1566, 1773
BstFNI CGCG 1 cut(s) 1666
BstHHI GCGC 2 cut(s) 144, 1666
BstMAI GTCTC 1 cut(s) 585
BstMCI CGRYCG 1 cut(s) 226
BstNI CCWGG 2 cut(s) 559, 596
BstNSI RCATGY 1 cut(s) 1508
BstSCI CCNGG 2 cut(s) 557, 594
BstSFI CTRYAG 3 cut(s) 918, 1272, 1632
BstUI CGCG 1 cut(s) 1666
BstV1I GCAGC 5 cut(s) 142, 775, 965, 1258, 1648
BstX2I RGATCY 2 cut(s) 776, 1484
BstXI CCANNNNNNTGG 1 cut(s) 1577
BstYI RGATCY 2 cut(s) 776, 1484
BstZI CGGCCG 1 cut(s) 223
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 3 cut(s) 225, 819, 1041
BtgI CCRYGG 3 cut(s) 145, 226, 327
BtgZI GCGATG 1 cut(s) 1488
BtrI CACGTC 1 cut(s) 1136
BtsCI GGATG 5 cut(s) 355, 772, 1408, 1566, 1773
BtsI GCAGTG 2 cut(s) 177, 994
BtsIMutI CAGTG 3 cut(s) 177, 994, 1006
BveI ACCTGC 2 cut(s) 733, 995
Cac8I GCNNGC 3 cut(s) 933, 1387, 1506
CfoI GCGC 2 cut(s) 144, 1666
Cfr13I GGNCC 5 cut(s) 80, 353, 722, 733, 1077
Csp6I GTAC 3 cut(s) 235, 652, 890
CviAII CATG 9 cut(s) 134, 146, 323, 328, 796, 965, 1382, 1505, 1519
CviQI GTAC 3 cut(s) 235, 652, 890
DdeI CTNAG 7 cut(s) 123, 392, 463, 471, 1128, 1225, 1398
DraI TTTAAA 2 cut(s) 864, 1420
EaeI YGGCCR 1 cut(s) 223
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 3 cut(s) 743, 1164, 1761
EarI CTCTTC 3 cut(s) 743, 1164, 1761
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 145, 327
Eco31I GGTCTC 1 cut(s) 585
Eco47I GGWCC 5 cut(s) 80, 353, 722, 733, 1077
Eco52I CGGCCG 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 291
Eco72I CACGTG 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 849
EcoNI CCTNNNNNAGG 1 cut(s) 740
EcoO109I RGGNCCY 1 cut(s) 722
EcoRII CCWGG 2 cut(s) 557, 594
EcoT14I CCWWGG 2 cut(s) 145, 327
EcoT22I ATGCAT 1 cut(s) 1372
ErhI CCWWGG 2 cut(s) 145, 327
FaeI CATG 9 cut(s) 137, 149, 326, 331, 799, 968, 1385, 1508, 1522
FalI AAGNNNNNCTT 4 cut(s) 876, 908, 1282, 1314
FaqI GGGAC 2 cut(s) 542, 1251
FatI CATG 9 cut(s) 133, 145, 322, 327, 795, 964, 1381, 1504, 1518
FauNDI CATATG 1 cut(s) 1366
FbaI TGATCA 3 cut(s) 1438, 1449, 1735
FblI GTMKAC 2 cut(s) 658, 1623
Fnu4HI GCNGC 6 cut(s) 131, 789, 957, 979, 1272, 1662
FokI GGATG 5 cut(s) 362, 779, 1415, 1553, 1780
Fsp4HI GCNGC 6 cut(s) 131, 789, 957, 979, 1272, 1662
FspBI CTAG 5 cut(s) 453, 642, 815, 932, 1721
GlaI GCGC 2 cut(s) 143, 1665
GluI GCNGC 6 cut(s) 131, 789, 957, 979, 1272, 1662
GsuI CTGGAG 2 cut(s) 617, 1599
HaeIII GGCC 3 cut(s) 225, 819, 1041
HapII CCGG 1 cut(s) 649
HhaI GCGC 2 cut(s) 144, 1666
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 9 cut(s) 137, 149, 326, 331, 799, 968, 1385, 1508, 1522
Hin6I GCGC 2 cut(s) 142, 1664
HinP1I GCGC 2 cut(s) 142, 1664
HincII GTYRAC 2 cut(s) 368, 1585
HindII GTYRAC 2 cut(s) 368, 1585
HindIII AAGCTT 1 cut(s) 1674
HinfI GANTC 8 cut(s) 257, 372, 449, 601, 674, 1515, 1628, 1643
HpaII CCGG 1 cut(s) 649
HphI GGTGA 1 cut(s) 1459
Hpy166II GTNNAC 5 cut(s) 216, 368, 659, 1585, 1624
Hpy188III TCNNGA 7 cut(s) 347, 453, 872, 1236, 1538, 1578, 1721
Hpy8I GTNNAC 5 cut(s) 216, 368, 659, 1585, 1624
Hpy99I CGWCG 3 cut(s) 31, 34, 1137
HpyAV CCTTC 4 cut(s) 391, 713, 1585, 1699
HpyCH4III ACNGT 5 cut(s) 265, 429, 656, 1340, 1493
HpyCH4IV ACGT 4 cut(s) 29, 99, 288, 1135
HpyF3I CTNAG 7 cut(s) 123, 392, 463, 471, 1128, 1225, 1398
HpySE526I ACGT 4 cut(s) 29, 99, 288, 1135
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 9 cut(s) 137, 149, 326, 331, 799, 968, 1385, 1508, 1522
HspAI GCGC 2 cut(s) 142, 1664
Ksp22I TGATCA 3 cut(s) 1438, 1449, 1735
LguI GCTCTTC 1 cut(s) 1164
LmnI GCTCC 3 cut(s) 318, 975, 1540
Lsp1109I GCAGC 5 cut(s) 142, 775, 965, 1258, 1648
LweI GCATC 2 cut(s) 117, 1393
MaeI CTAG 5 cut(s) 453, 642, 815, 932, 1721
MaeII ACGT 4 cut(s) 29, 99, 288, 1135
MaeIII GTNAC 2 cut(s) 265, 1088
MboII GAAGA 4 cut(s) 730, 1181, 1748, 1783
MfeI CAATTG 1 cut(s) 1713
MflI RGATCY 2 cut(s) 776, 1484
MlyI GAGTC 1 cut(s) 683
Mph1103I ATGCAT 1 cut(s) 1372
MseI TTAA 3 cut(s) 863, 1287, 1419
MslI CAYNNNNRTG 1 cut(s) 225
MspA1I CMGCKG 2 cut(s) 109, 1211
MspI CCGG 1 cut(s) 649
MspR9I CCNGG 2 cut(s) 559, 596
MunI CAATTG 1 cut(s) 1713
Mva1269I GAATGC 3 cut(s) 844, 981, 1484
MvaI CCWGG 2 cut(s) 559, 596
MvnI CGCG 1 cut(s) 1666
NcoI CCATGG 2 cut(s) 145, 327
NdeI CATATG 1 cut(s) 1366
NheI GCTAGC 1 cut(s) 931
NlaIII CATG 9 cut(s) 137, 149, 326, 331, 799, 968, 1385, 1508, 1522
NlaIV GGNNCC 2 cut(s) 320, 500
NmeAIII GCCGAG 1 cut(s) 269
NsiI ATGCAT 1 cut(s) 1372
NspI RCATGY 1 cut(s) 1508
NspV TTCGAA 1 cut(s) 836
OliI CACNNNNGTG 1 cut(s) 225
PaeI GCATGC 1 cut(s) 1508
PciSI GCTCTTC 1 cut(s) 1164
PctI GAATGC 3 cut(s) 844, 981, 1484
PfeI GAWTC 7 cut(s) 257, 372, 449, 601, 1515, 1628, 1643
PflFI GACNNNGTC 2 cut(s) 28, 1226
PflMI CCANNNNNTGG 1 cut(s) 328
PkrI GCNGC 6 cut(s) 132, 790, 958, 980, 1273, 1663
PleI GAGTC 1 cut(s) 682
PmaCI CACGTG 1 cut(s) 289
PmlI CACGTG 1 cut(s) 289
PpsI GAGTC 1 cut(s) 682
Ppu21I YACGTR 1 cut(s) 289
PpuMI RGGWCCY 1 cut(s) 722
Psp1406I AACGTT 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 2 cut(s) 557, 594
PspCI CACGTG 1 cut(s) 289
PspGI CCWGG 2 cut(s) 557, 594
PspN4I GGNNCC 2 cut(s) 320, 500
PspPI GGNCC 5 cut(s) 80, 353, 722, 733, 1077
PspPPI RGGWCCY 1 cut(s) 722
PstI CTGCAG 2 cut(s) 1276, 1636
PsuI RGATCY 2 cut(s) 776, 1484
PsyI GACNNNGTC 2 cut(s) 28, 1226
PvuII CAGCTG 2 cut(s) 109, 1211
RsaI GTAC 3 cut(s) 236, 653, 891
RsaNI GTAC 3 cut(s) 235, 652, 890
RseI CAYNNNNRTG 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 1164
SaqAI TTAA 3 cut(s) 863, 1287, 1419
SatI GCNGC 6 cut(s) 131, 789, 957, 979, 1272, 1662
Sau96I GGNCC 5 cut(s) 80, 353, 722, 733, 1077
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 2 cut(s) 559, 596
SfaNI GCATC 2 cut(s) 117, 1393
SfcI CTRYAG 3 cut(s) 918, 1272, 1632
SfuI TTCGAA 1 cut(s) 836
SinI GGWCC 5 cut(s) 80, 353, 722, 733, 1077
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 3 cut(s) 870, 1607, 1616
SmoI CTYRAG 3 cut(s) 870, 1607, 1616
SphI GCATGC 1 cut(s) 1508
SsiI CCGC 4 cut(s) 241, 563, 957, 1180
SspMI CTAG 5 cut(s) 453, 642, 815, 932, 1721
StyD4I CCNGG 2 cut(s) 557, 594
StyI CCWWGG 2 cut(s) 145, 327
TaaI ACNGT 5 cut(s) 265, 429, 656, 1340, 1493
TaiI ACGT 4 cut(s) 32, 102, 291, 1138
TaqI TCGA 3 cut(s) 45, 520, 836
TaqII GACCGA 1 cut(s) 68
TatI WGTACW 2 cut(s) 234, 889
TauI GCSGC 1 cut(s) 959
TfiI GAWTC 7 cut(s) 257, 372, 449, 601, 1515, 1628, 1643
Tru1I TTAA 3 cut(s) 863, 1287, 1419
Tru9I TTAA 3 cut(s) 863, 1287, 1419
TscAI CASTG 3 cut(s) 184, 994, 1006
TseI GCWGC 5 cut(s) 130, 788, 978, 1271, 1661
TspDTI ATGAA 6 cut(s) 506, 797, 888, 1491, 1780, 1784
TspGWI ACGGA 2 cut(s) 603, 1090
TspRI CASTG 3 cut(s) 184, 994, 1006
Tth111I GACNNNGTC 2 cut(s) 28, 1226
Van91I CCANNNNNTGG 1 cut(s) 328
VpaK11BI GGWCC 5 cut(s) 80, 353, 722, 733, 1077
XagI CCTNNNNNAGG 1 cut(s) 740
XapI RAATTY 5 cut(s) 86, 803, 948, 1415, 1598
XbaI TCTAGA 2 cut(s) 452, 1720
XceI RCATGY 1 cut(s) 1508
XmiI GTMKAC 2 cut(s) 658, 1623
XspI CTAG 5 cut(s) 453, 642, 815, 932, 1721
ZraI GACGTC 1 cut(s) 30
Zsp2I ATGCAT 1 cut(s) 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.