Rh7BG100300

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006889
Physical Location & Seq
Forward (+)
7435218 .. 7440668
5451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG100300.1

Sequence Viewer

Length: 1722 bp
ATGGGGAAAGAGAACCAGAACCAGAACCAGAACCAGACGACGTCGGCTATGGAGATCGACAATCAAAAGGCGAACACCTCCGATCAGATCGGTCCTAAATTCTCAATCAACGTTTTACAGCTGTTGAAATCTGCTCAGATGCAGCATGGATTGCGCCATGGGGATTACACTCGTTATCGGAGGTATTGCACTGCAAGGTTGAGGAGGCTGTATAAGTCATTGAAGTTCACTCACGGCCGTGGCAAGTACAACCGCCGAGCAATAACCGAATCAACTGTTACTGAAGTGAGGTTTCTCCATGTGGTTCTATATACGGCAGAGAGGGCTTGGAGCCATGCCATGGAAAAAAGGCAAGTTCTGGATGGTCCAAATGCTCGTCAACGAATCTATCTGATTGGTAGGCTGAGGAAGGCAGTAAAATGGGCTACTCTTTTTGCACAGTTGTCTGCAATCAAGGGAGATTCTAGAACTTCCTTAGAAGCTGAGGCTTATGCCTCTTTTATGAAAGGGAACCTGTTGTTTGAACAAGATCGAAATTGGGACACAGCATTACTGAACTTCAAAAGTGCCAGGGCGGTTTATGAGGAACTTGCGAAATATGGAGACCTGGAGAATCAAGTTTTATGCCGTGAGCGTGTTGAGGAATTAGAACCTAGTATCCGGTACTGTCTACACAAGATTGGAGAGTCAAATCTACAAGGCTCTGAACTTCTACATATTGGTGAGATGGAAGGACCTGCTTTGGACCTCTTCAAGGCTAAATTGGAGGCTGTTATGGATGAGGCAAGATCTCAACAGGCTGCCTCCATGACAGAATTTCATTGGCTAGGCCATAGATTTCCAATTTCAAATGCAAAAACTCGGGTTTCTATTTTAAAAGCTCAAGACTTGGAGAAAGATGTACTTGGTTCATCAGCTAATTCACTCCCTACAGAGAAAAGGCTAGCCATTTTTGACAAAGTTTTCACTGCATACCATGAGGCAAGGAGCTGCATTCGCAGTGATTTGGTCAGTGCAGGTAGTTCTGAAAATGTCAAAGATGACTTGAATGGCCTTGATAAAGCTGTTAGTGCTGTATTAGGACAACGGACCATTGAGCGTAACCTACTTTTGGTTAGCATTGCAAAGAGCAAACTCACTAAGCGACGAGATGACAAAAATGAGAGAACTACGAAGCCAGAAGAGCTTGTCCGCTTGTATGATCTCCTATTACAGAATACAGCTGACCTTTCAGATTTAGTCAGTTCGGGACGAGAAAAAACATCAGAGGAAGTCGCATTTGCTGCAGAATGTGAGCTTAAAAGTTTGGCTTTCCGAGCAGAAAGGTGTTTCTTTTTGGGTAGATCATACAGTTTGGCTGGTAAGAGGGTGGAAGCATATGCATTGTATTGCCGTGCTCGCACACTTGCTGAGGATGCCCTGCAAAAATTTAAAGCCGTTGATAATGCTGATCAGGTGGTGATCAAAGAGTTGAAGCTATTGTGCGATGAATGCAGATCTAACAGTTGTATAGAGCATGCAACAGGAATCATGGAGGAGCTGAAGGCTCCTGAAAATCTCTCACAACAGATTTCTAACATCCATATATCTGGAGTTGACACAAAGGTATGCATTATGCTTTTCCTCTTCGGCAGGAAATATTTGAATGATGTCATGAACCTCCATAGTTTAGTAGCACTGGCCAAGGAGATTTTGTGTCCTGGTAATCATTCTTCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
SRP68

Protein Analysis

573

Amino Acids

64.89

Weight (kDa)

8.87

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP68 PF16969 51 - 525 5.6e-143 RNA-binding signal recognition particle 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 44
Acc36I ACCTGC 2 cut(s) 745, 1007
AccB7I CCANNNNNTGG 1 cut(s) 340
AccI GTMKAC 1 cut(s) 670
AciI CCGC 3 cut(s) 253, 575, 1192
AclI AACGTT 1 cut(s) 111
AcoI YGGCCR 2 cut(s) 235, 1680
AcsI RAATTY 3 cut(s) 98, 815, 1427
AcuI CTGAAG 2 cut(s) 303, 1562
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 3 cut(s) 248, 665, 903
AfiI CCNNNNNNNGG 3 cut(s) 340, 754, 1111
AjnI CCWGG 3 cut(s) 569, 606, 1699
AleI CACNNNNGTG 1 cut(s) 237
AloI GAACNNNNNNTCC 2 cut(s) 642, 674
Alw21I GWGCWC 1 cut(s) 1399
Alw26I GTCTC 1 cut(s) 597
Ama87I CYCGRG 1 cut(s) 861
AoxI GGCC 4 cut(s) 235, 829, 1051, 1680
ApeKI GCWGC 4 cut(s) 142, 800, 990, 1283
ApoI RAATTY 3 cut(s) 98, 815, 1427
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 5 cut(s) 92, 365, 734, 745, 1089
AsuHPI GGTGA 2 cut(s) 734, 1471
AsuNHI GCTAGC 1 cut(s) 943
AvaI CYCGRG 1 cut(s) 861
AvaII GGWCC 5 cut(s) 92, 365, 734, 745, 1089
BalI TGGCCA 1 cut(s) 1682
BarI GAAGNNNNNNTAC 1 cut(s) 1696
Bbv12I GWGCWC 1 cut(s) 1399
BbvCI CCTCAGC 3 cut(s) 404, 483, 1410
BbvI GCAGC 4 cut(s) 154, 787, 977, 1270
BccI CCATC 2 cut(s) 356, 721
BceAI ACGGC 6 cut(s) 222, 250, 330, 612, 1377, 1421
BciT130I CCWGG 3 cut(s) 571, 608, 1701
BciVI GTATCC 1 cut(s) 668
BclI TGATCA 2 cut(s) 1450, 1461
BcoDI GTCTC 1 cut(s) 597
BfaI CTAG 4 cut(s) 465, 654, 827, 944
BfmI CTRYAG 2 cut(s) 930, 1284
BfuAI ACCTGC 2 cut(s) 745, 1007
BfuI GTATCC 1 cut(s) 668
BglII AGATCT 2 cut(s) 788, 1496
BisI GCNGC 4 cut(s) 143, 801, 991, 1284
BlsI GCNGC 4 cut(s) 144, 802, 992, 1285
Bme1390I CCNGG 3 cut(s) 571, 608, 1701
Bme18I GGWCC 5 cut(s) 92, 365, 734, 745, 1089
BmeT110I CYCGRG 1 cut(s) 861
BmgT120I GGNCC 5 cut(s) 92, 365, 734, 745, 1089
BmiI GGNNCC 3 cut(s) 332, 512, 1548
BmrFI CCNGG 3 cut(s) 571, 608, 1701
BmsI GCATC 2 cut(s) 129, 1405
BmtI GCTAGC 1 cut(s) 947
BpmI CTGGAG 2 cut(s) 629, 1611
Bpu10I CCTNAGC 3 cut(s) 404, 483, 1410
BpuEI CTTGAG 1 cut(s) 867
BsaHI GRCGYC 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 597
BsaJI CCNNGG 5 cut(s) 157, 238, 339, 570, 1683
BsaWI WCCGGW 1 cut(s) 660
BsaXI ACNNNNNCTCC 2 cut(s) 1679, 1709
Bsc4I CCNNNNNNNGG 3 cut(s) 340, 754, 1111
Bse1I ACTGG 1 cut(s) 1683
Bse3DI GCAATG 1 cut(s) 1119
BseBI CCWGG 3 cut(s) 571, 608, 1701
BseDI CCNNGG 5 cut(s) 157, 238, 339, 570, 1683
BseGI GGATG 4 cut(s) 367, 784, 1420, 1578
BseLI CCNNNNNNNGG 3 cut(s) 340, 754, 1111
BseMI GCAATG 1 cut(s) 1119
BseMII CTCAG 4 cut(s) 149, 395, 474, 1401
BseNI ACTGG 1 cut(s) 1683
BseRI GAGGAG 2 cut(s) 217, 1550
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 4 cut(s) 154, 787, 977, 1270
BsgI GTGCAG 1 cut(s) 1035
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 4 cut(s) 237, 831, 1053, 1682
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 1399
BsiHKCI CYCGRG 1 cut(s) 861
BsiSI CCGG 1 cut(s) 661
BslFI GGGAC 2 cut(s) 554, 1263
BslI CCNNNNNNNGG 3 cut(s) 340, 754, 1111
BsmAI GTCTC 1 cut(s) 597
BsmFI GGGAC 2 cut(s) 554, 1263
BsmI GAATGC 2 cut(s) 993, 1496
BsnI GGCC 4 cut(s) 237, 831, 1053, 1682
Bso31I GGTCTC 1 cut(s) 597
BsoBI CYCGRG 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 1399
Bsp19I CCATGG 2 cut(s) 157, 339
BspACI CCGC 3 cut(s) 253, 575, 1192
BspANI GGCC 4 cut(s) 237, 831, 1053, 1682
BspCNI CTCAG 4 cut(s) 148, 396, 475, 1402
BspHI TCATGA 1 cut(s) 1653
BspLI GGNNCC 3 cut(s) 332, 512, 1548
BspMAI CTGCAG 1 cut(s) 1288
BspMI ACCTGC 2 cut(s) 745, 1007
BspOI GCTAGC 1 cut(s) 947
BspQI GCTCTTC 1 cut(s) 1176
BspTNI GGTCTC 1 cut(s) 597
BsrDI GCAATG 1 cut(s) 1119
BsrI ACTGG 1 cut(s) 1683
BssECI CCNNGG 5 cut(s) 157, 238, 339, 570, 1683
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 3 cut(s) 157, 339, 1683
Bst2UI CCWGG 3 cut(s) 571, 608, 1701
Bst4CI ACNGT 5 cut(s) 277, 441, 668, 1352, 1505
Bst6I CTCTTC 3 cut(s) 755, 1176, 1631
BstACI GRCGYC 1 cut(s) 41
BstAPI GCANNNNNTGC 2 cut(s) 151, 1283
BstC8I GCNNGC 3 cut(s) 945, 1399, 1518
BstDEI CTNAG 6 cut(s) 135, 404, 475, 483, 1140, 1410
BstDSI CCRYGG 3 cut(s) 157, 238, 339
BstENI CCTNNNNNAGG 1 cut(s) 752
BstF5I GGATG 4 cut(s) 367, 784, 1420, 1578
BstHHI GCGC 1 cut(s) 156
BstMAI GTCTC 1 cut(s) 597
BstMCI CGRYCG 1 cut(s) 238
BstNI CCWGG 3 cut(s) 571, 608, 1701
BstNSI RCATGY 1 cut(s) 1520
BstSCI CCNGG 3 cut(s) 569, 606, 1699
BstSFI CTRYAG 2 cut(s) 930, 1284
BstV1I GCAGC 4 cut(s) 154, 787, 977, 1270
BstX2I RGATCY 2 cut(s) 788, 1496
BstXI CCANNNNNNTGG 1 cut(s) 1589
BstYI RGATCY 2 cut(s) 788, 1496
BstZI CGGCCG 1 cut(s) 235
BsuI GTATCC 1 cut(s) 668
BsuRI GGCC 4 cut(s) 237, 831, 1053, 1682
BtgI CCRYGG 3 cut(s) 157, 238, 339
BtgZI GCGATG 1 cut(s) 1500
BtsCI GGATG 4 cut(s) 367, 784, 1420, 1578
BtsI GCAGTG 3 cut(s) 189, 966, 1006
BtsIMutI CAGTG 5 cut(s) 189, 966, 1006, 1018, 1676
BveI ACCTGC 2 cut(s) 745, 1007
Cac8I GCNNGC 3 cut(s) 945, 1399, 1518
CciI TCATGA 1 cut(s) 1653
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 5 cut(s) 92, 365, 734, 745, 1089
Csp6I GTAC 3 cut(s) 247, 664, 902
CviQI GTAC 3 cut(s) 247, 664, 902
DdeI CTNAG 6 cut(s) 135, 404, 475, 483, 1140, 1410
DraI TTTAAA 2 cut(s) 876, 1432
EaeI YGGCCR 2 cut(s) 235, 1680
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 3 cut(s) 755, 1176, 1631
EarI CTCTTC 3 cut(s) 755, 1176, 1631
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 157, 339, 1683
Eco31I GGTCTC 1 cut(s) 597
Eco47I GGWCC 5 cut(s) 92, 365, 734, 745, 1089
Eco52I CGGCCG 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 303, 1562
Eco88I CYCGRG 1 cut(s) 861
EcoNI CCTNNNNNAGG 1 cut(s) 752
EcoO109I RGGNCCY 1 cut(s) 734
EcoRII CCWGG 3 cut(s) 569, 606, 1699
EcoT14I CCWWGG 3 cut(s) 157, 339, 1683
EcoT22I ATGCAT 2 cut(s) 1384, 1613
ErhI CCWWGG 3 cut(s) 157, 339, 1683
FalI AAGNNNNNCTT 4 cut(s) 888, 920, 1294, 1326
FaqI GGGAC 2 cut(s) 554, 1263
FauNDI CATATG 1 cut(s) 1378
FbaI TGATCA 2 cut(s) 1450, 1461
FblI GTMKAC 1 cut(s) 670
Fnu4HI GCNGC 4 cut(s) 143, 801, 991, 1284
FokI GGATG 4 cut(s) 374, 791, 1427, 1565
Fsp4HI GCNGC 4 cut(s) 143, 801, 991, 1284
FspBI CTAG 4 cut(s) 465, 654, 827, 944
GlaI GCGC 1 cut(s) 155
GluI GCNGC 4 cut(s) 143, 801, 991, 1284
GsuI CTGGAG 2 cut(s) 629, 1611
HaeIII GGCC 4 cut(s) 237, 831, 1053, 1682
HapII CCGG 1 cut(s) 661
HhaI GCGC 1 cut(s) 156
Hin1I GRCGYC 1 cut(s) 41
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HincII GTYRAC 2 cut(s) 380, 1597
HindII GTYRAC 2 cut(s) 380, 1597
HinfI GANTC 6 cut(s) 269, 384, 461, 613, 686, 1527
HpaII CCGG 1 cut(s) 661
HphI GGTGA 2 cut(s) 734, 1471
Hpy166II GTNNAC 4 cut(s) 228, 380, 671, 1597
Hpy188III TCNNGA 7 cut(s) 359, 465, 884, 1248, 1550, 1590, 1654
Hpy8I GTNNAC 4 cut(s) 228, 380, 671, 1597
Hpy99I CGWCG 3 cut(s) 43, 46, 1149
HpyAV CCTTC 3 cut(s) 403, 725, 1537
HpyCH4III ACNGT 5 cut(s) 277, 441, 668, 1352, 1505
HpyCH4IV ACGT 2 cut(s) 41, 111
HpyF3I CTNAG 6 cut(s) 135, 404, 475, 483, 1140, 1410
HpySE526I ACGT 2 cut(s) 41, 111
Hsp92I GRCGYC 1 cut(s) 41
HspAI GCGC 1 cut(s) 154
Ksp22I TGATCA 2 cut(s) 1450, 1461
LguI GCTCTTC 1 cut(s) 1176
LmnI GCTCC 4 cut(s) 330, 987, 1537, 1552
Lsp1109I GCAGC 4 cut(s) 154, 787, 977, 1270
LweI GCATC 2 cut(s) 129, 1405
MaeI CTAG 4 cut(s) 465, 654, 827, 944
MaeII ACGT 2 cut(s) 41, 111
MaeIII GTNAC 2 cut(s) 277, 1100
MboII GAAGA 4 cut(s) 742, 1193, 1618, 1704
MflI RGATCY 2 cut(s) 788, 1496
MhlI GDGCHC 1 cut(s) 1399
MlsI TGGCCA 1 cut(s) 1682
MluCI AATT 9 cut(s) 98, 535, 644, 761, 815, 843, 919, 1427, 1716
MluNI TGGCCA 1 cut(s) 1682
MlyI GAGTC 1 cut(s) 695
Mox20I TGGCCA 1 cut(s) 1682
Mph1103I ATGCAT 2 cut(s) 1384, 1613
MscI TGGCCA 1 cut(s) 1682
MseI TTAA 3 cut(s) 875, 1299, 1431
MslI CAYNNNNRTG 2 cut(s) 237, 720
Msp20I TGGCCA 1 cut(s) 1682
MspA1I CMGCKG 2 cut(s) 121, 1223
MspI CCGG 1 cut(s) 661
MspR9I CCNGG 3 cut(s) 571, 608, 1701
Mva1269I GAATGC 2 cut(s) 993, 1496
MvaI CCWGG 3 cut(s) 571, 608, 1701
NcoI CCATGG 2 cut(s) 157, 339
NdeI CATATG 1 cut(s) 1378
NheI GCTAGC 1 cut(s) 943
NlaIV GGNNCC 3 cut(s) 332, 512, 1548
NmeAIII GCCGAG 1 cut(s) 281
NsiI ATGCAT 2 cut(s) 1384, 1613
NspI RCATGY 1 cut(s) 1520
OliI CACNNNNGTG 1 cut(s) 237
PaeI GCATGC 1 cut(s) 1520
PagI TCATGA 1 cut(s) 1653
PciSI GCTCTTC 1 cut(s) 1176
PctI GAATGC 2 cut(s) 993, 1496
PfeI GAWTC 5 cut(s) 269, 384, 461, 613, 1527
PflFI GACNNNGTC 1 cut(s) 40
PflMI CCANNNNNTGG 1 cut(s) 340
PkrI GCNGC 4 cut(s) 144, 802, 992, 1285
PleI GAGTC 1 cut(s) 694
PpsI GAGTC 1 cut(s) 694
PpuMI RGGWCCY 1 cut(s) 734
Psp1406I AACGTT 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 734
Psp6I CCWGG 3 cut(s) 569, 606, 1699
PspGI CCWGG 3 cut(s) 569, 606, 1699
PspN4I GGNNCC 3 cut(s) 332, 512, 1548
PspPI GGNCC 5 cut(s) 92, 365, 734, 745, 1089
PspPPI RGGWCCY 1 cut(s) 734
PstI CTGCAG 1 cut(s) 1288
PsuI RGATCY 2 cut(s) 788, 1496
PsyI GACNNNGTC 1 cut(s) 40
PvuII CAGCTG 2 cut(s) 121, 1223
RsaI GTAC 3 cut(s) 248, 665, 903
RsaNI GTAC 3 cut(s) 247, 664, 902
RseI CAYNNNNRTG 2 cut(s) 237, 720
SapI GCTCTTC 1 cut(s) 1176
SaqAI TTAA 3 cut(s) 875, 1299, 1431
SatI GCNGC 4 cut(s) 143, 801, 991, 1284
Sau96I GGNCC 5 cut(s) 92, 365, 734, 745, 1089
SchI GAGTC 1 cut(s) 695
ScrFI CCNGG 3 cut(s) 571, 608, 1701
SduI GDGCHC 1 cut(s) 1399
SfaNI GCATC 2 cut(s) 129, 1405
SfcI CTRYAG 2 cut(s) 930, 1284
SinI GGWCC 5 cut(s) 92, 365, 734, 745, 1089
SmiMI CAYNNNNRTG 2 cut(s) 237, 720
SmlI CTYRAG 1 cut(s) 882
SmoI CTYRAG 1 cut(s) 882
SphI GCATGC 1 cut(s) 1520
Sse9I AATT 9 cut(s) 98, 535, 644, 761, 815, 843, 919, 1427, 1716
SsiI CCGC 3 cut(s) 253, 575, 1192
SspI AATATT 1 cut(s) 1640
SspMI CTAG 4 cut(s) 465, 654, 827, 944
StyD4I CCNGG 3 cut(s) 569, 606, 1699
StyI CCWWGG 3 cut(s) 157, 339, 1683
TaaI ACNGT 5 cut(s) 277, 441, 668, 1352, 1505
TaiI ACGT 2 cut(s) 44, 114
TaqI TCGA 2 cut(s) 57, 532
TaqII GACCGA 1 cut(s) 80
TasI AATT 9 cut(s) 98, 535, 644, 761, 815, 843, 919, 1427, 1716
TatI WGTACW 2 cut(s) 246, 901
TfiI GAWTC 5 cut(s) 269, 384, 461, 613, 1527
Tru1I TTAA 3 cut(s) 875, 1299, 1431
Tru9I TTAA 3 cut(s) 875, 1299, 1431
TscAI CASTG 5 cut(s) 196, 973, 1006, 1018, 1683
TseI GCWGC 4 cut(s) 142, 800, 990, 1283
TspDTI ATGAA 5 cut(s) 518, 809, 900, 1503, 1670
TspGWI ACGGA 1 cut(s) 1102
TspRI CASTG 5 cut(s) 196, 973, 1006, 1018, 1683
Tth111I GACNNNGTC 1 cut(s) 40
Van91I CCANNNNNTGG 1 cut(s) 340
VpaK11BI GGWCC 5 cut(s) 92, 365, 734, 745, 1089
XagI CCTNNNNNAGG 1 cut(s) 752
XapI RAATTY 3 cut(s) 98, 815, 1427
XbaI TCTAGA 1 cut(s) 464
XceI RCATGY 1 cut(s) 1520
XmiI GTMKAC 1 cut(s) 670
XspI CTAG 4 cut(s) 465, 654, 827, 944
ZraI GACGTC 1 cut(s) 42
Zsp2I ATGCAT 2 cut(s) 1384, 1613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.