RLG00000004660

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006889
Physical Location & Seq
Reverse (-)
61230836 .. 61235950
5115 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004660

Sequence Viewer

Length: 2004 bp
ATGGCTCATTTAAACAGTGTAGGCCCAAGTACAAGAATAGGCCCAGATTTGCCAGCCCACTTCCAAATTATTCCCAATTTGCCTATACCGTCGAATTTAGATAGAAATAACGGACCAAGGTCCAAAGCCAAATCAAAGAAACTACAGGGGTCACTGCGAAACTGCCGCCGGAGCAATCTCAGGATATTTGAAATGGGGAAAGAGAACCACAACCAGACGACGTCGGCTATGGAGATCGACAATCAAAAGGCGAACACCTCCGATCAGATCGGTCCTAAATTCTCAATCAACGTTTTACAGCTGTTGAAATCTGCTCAGATGCAGCATGGATTGCGCCATGGGGATTACACTCGTTATCGGAGGTATTGCACTGCAAGGTTGAGGAGGCTGTATAAGTCATTGAAGTTCACTCACGGCCGTGGCAAGTACAACCGCCGAGCAATAACCGAATCAACTGTTACTGAAGTGAGGTTTCTCCATGTGGTTCTATATACGGCAGAGAGGGCTTGGAGCCATGCCATGGAAAAAAGGCAAGTTCTGGATGGTCCAAATGCTCGTCAACGAATCTATCTGATTGGTAGGCTGAGGAAGGCAGTAAAATGGGCTACTCTTTTTGCACAGTTGTCTGCAATCAAGGGAGATTCTAGAACTTCCTTAGAAGCTGAGGCTTATGCCTCTTTTATGAAAGGGAACCTGTTGTTTGAACAAGATCGAAATTGGGACACAGCATTACTGAACTTCAAAAGTGCCAGGGCGGTTTATGAGGAACTTGCGAAATATGGAGACCTGGAGAATCAAGTTTTATGCCGTGAGCGTGTTGAGGAATTAGAACCCAGTATCCGGTACTGTCTACACAAGATTGGAGAGTCAAATCTACAAGGCTCTGAACTTCTACATATTGGCGAGATGGAAGGACCTGCTTTGGACCTCTTCAAGGCTAAATTGGAGGCTGTTATGGATGAGGCAAGATCTCAACAGGCTGCCTCCATGACAGAATTTCATTGGCTAGGCCATAGATTTCCAATTTCGAATGCAAAAACTCGGGTTTCTATTTTAAAAGCTCAAGACTTGGAGAAAGATGTACTTGGTTCATCAGCTAATTCACTCCCTACAGAGAAAAGGCTAGCCATTTTTGACAAAATTTTCACTGCATACCATGAGGCAAGGAGCTGCATTCGCAGTGATTTGGTCAGTGCAGGTAGTTCTGAAAATGTCAAAGATGACTTGAATGGCCTTGATAAAGCTGTTAGTGCTGTATTAGGACAACGGACCATTGAGCGTAACCTACTTTTGGTTAGCATTGCAAAGAGCAAACTCACTAAGCGACGAGATGACAAAAATGAGAGAACTACGAAGCCAGAAGAGCTTGTCCGCTTGTATGATCTCCTATTACAGAATACAGCTGACCTTTCAGATTTAGTCAGTTCGGGACGAGAAAAAACATCAGAGGAAGTCGCATTTGCTGCAGAATGTGAGCTTAAAAGTTTGGCTTTCCGAGCAGAAAGGTGTTTCTTTTTGGGTAGCTCATACAGTTTGGCTGGTAAGAGGGTGGAAGCATATGCATTGTATTGCCGTGCTCGCACACTTGCTGAGGATGCCCTGCAAAAATTTAAAGCTGTTGCTAATGCTGATCAGGTGGTGATCAAGGAGTTGAAGCTATTGTGCGATGAATGCAGATCTAACAGTTGTATAGAGCATGCAACAGGAATCATGGAGGAGCTGAAGGCTCCTGAAAATCTCTCACAACAGATTTCTAACATCCATATATCTGGTGTAGACACGAAGGAGAAATTCCTCCTTGAGAAACTTGAGGTCTACGAATCTGCAGTTGGTGATTCAAATGTGAGAAGTGCTGCACGAATTGAAGCCTTCCCTCCTCCCTTCCAAACAATTCCTCGCAATCCAATTGTTCTAGACCTTGCCTATGATCATATTGAGTTCCCATCTCTTCTAAATAGGATGAAGAAAGAAACAAAGGGCTTCATTAGTAGGTTTTGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
SRP68

Protein Analysis

668

Amino Acids

75.59

Weight (kDa)

9.25

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP68 PF16969 111 - 645 7.6e-159 RNA-binding signal recognition particle 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 224
Acc36I ACCTGC 2 cut(s) 925, 1187
AccB7I CCANNNNNTGG 1 cut(s) 520
AccI GTMKAC 3 cut(s) 850, 1776, 1815
AciI CCGC 4 cut(s) 166, 433, 755, 1372
AclI AACGTT 1 cut(s) 291
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 6 cut(s) 94, 278, 995, 1140, 1607, 1790
AcuI CTGAAG 2 cut(s) 483, 1742
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 4 cut(s) 31, 428, 845, 1083
AfiI CCNNNNNNNGG 4 cut(s) 520, 840, 934, 1291
AjnI CCWGG 2 cut(s) 749, 786
AjuI GAANNNNNNNTTGG 2 cut(s) 1812, 1844
AleI CACNNNNGTG 1 cut(s) 417
AloI GAACNNNNNNTCC 2 cut(s) 822, 854
Alw21I GWGCWC 1 cut(s) 1579
Alw26I GTCTC 1 cut(s) 777
Ama87I CYCGRG 1 cut(s) 1041
AoxI GGCC 5 cut(s) 22, 40, 415, 1009, 1231
ApeKI GCWGC 5 cut(s) 322, 980, 1170, 1463, 1853
ApoI RAATTY 6 cut(s) 94, 278, 995, 1140, 1607, 1790
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 9 cut(s) 23, 41, 113, 120, 272, 545, 914, 925, 1269
AsuHPI GGTGA 2 cut(s) 1651, 1844
AsuII TTCGAA 1 cut(s) 1028
AsuNHI GCTAGC 1 cut(s) 1123
AvaI CYCGRG 1 cut(s) 1041
AvaII GGWCC 7 cut(s) 113, 120, 272, 545, 914, 925, 1269
Bbv12I GWGCWC 1 cut(s) 1579
BbvCI CCTCAGC 3 cut(s) 584, 663, 1590
BbvI GCAGC 5 cut(s) 334, 967, 1157, 1450, 1840
BccI CCATC 3 cut(s) 536, 901, 1951
BceAI ACGGC 5 cut(s) 402, 430, 510, 792, 1557
BciT130I CCWGG 2 cut(s) 751, 788
BciVI GTATCC 1 cut(s) 848
BclI TGATCA 3 cut(s) 1630, 1641, 1927
BcoDI GTCTC 1 cut(s) 777
BfaI CTAG 4 cut(s) 645, 1007, 1124, 1913
BfmI CTRYAG 4 cut(s) 143, 1110, 1464, 1824
BfuAI ACCTGC 2 cut(s) 925, 1187
BfuI GTATCC 1 cut(s) 848
BglII AGATCT 2 cut(s) 968, 1676
BisI GCNGC 6 cut(s) 166, 323, 981, 1171, 1464, 1854
BlsI GCNGC 6 cut(s) 167, 324, 982, 1172, 1465, 1855
Bme1390I CCNGG 2 cut(s) 751, 788
Bme18I GGWCC 7 cut(s) 113, 120, 272, 545, 914, 925, 1269
BmeT110I CYCGRG 1 cut(s) 1041
BmgT120I GGNCC 9 cut(s) 23, 41, 113, 120, 272, 545, 914, 925, 1269
BmiI GGNNCC 3 cut(s) 512, 692, 1728
BmrFI CCNGG 2 cut(s) 751, 788
BmrI ACTGGG 1 cut(s) 828
BmsI GCATC 2 cut(s) 309, 1585
BmtI GCTAGC 1 cut(s) 1127
BmuI ACTGGG 1 cut(s) 828
BoxI GACNNNNGTC 1 cut(s) 118
BpmI CTGGAG 1 cut(s) 809
Bpu10I CCTNAGC 3 cut(s) 584, 663, 1590
Bpu14I TTCGAA 1 cut(s) 1028
BpuEI CTTGAG 3 cut(s) 1047, 1820, 1829
BsaHI GRCGYC 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 5 cut(s) 116, 337, 418, 519, 750
BsaWI WCCGGW 1 cut(s) 840
Bsc4I CCNNNNNNNGG 4 cut(s) 520, 840, 934, 1291
Bse1I ACTGG 1 cut(s) 834
Bse3DI GCAATG 1 cut(s) 1299
BseBI CCWGG 2 cut(s) 751, 788
BseDI CCNNGG 5 cut(s) 116, 337, 418, 519, 750
BseGI GGATG 5 cut(s) 547, 964, 1600, 1758, 1965
BseLI CCNNNNNNNGG 4 cut(s) 520, 840, 934, 1291
BseMI GCAATG 1 cut(s) 1299
BseMII CTCAG 5 cut(s) 193, 329, 575, 654, 1581
BseNI ACTGG 1 cut(s) 834
BseRI GAGGAG 3 cut(s) 397, 1730, 1866
BseX3I CGGCCG 1 cut(s) 415
BseXI GCAGC 5 cut(s) 334, 967, 1157, 1450, 1840
BsgI GTGCAG 2 cut(s) 1215, 1839
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 5 cut(s) 24, 42, 417, 1011, 1233
BsiEI CGRYCG 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 1579
BsiHKCI CYCGRG 1 cut(s) 1041
BsiSI CCGG 2 cut(s) 169, 841
BslFI GGGAC 2 cut(s) 734, 1443
BslI CCNNNNNNNGG 4 cut(s) 520, 840, 934, 1291
BsmAI GTCTC 1 cut(s) 777
BsmFI GGGAC 2 cut(s) 734, 1443
BsmI GAATGC 3 cut(s) 1036, 1173, 1676
BsnI GGCC 5 cut(s) 24, 42, 417, 1011, 1233
Bso31I GGTCTC 1 cut(s) 777
BsoBI CYCGRG 1 cut(s) 1041
Bsp119I TTCGAA 1 cut(s) 1028
Bsp1286I GDGCHC 1 cut(s) 1579
Bsp19I CCATGG 2 cut(s) 337, 519
BspACI CCGC 4 cut(s) 166, 433, 755, 1372
BspANI GGCC 5 cut(s) 24, 42, 417, 1011, 1233
BspCNI CTCAG 5 cut(s) 192, 328, 576, 655, 1582
BspLI GGNNCC 3 cut(s) 512, 692, 1728
BspMAI CTGCAG 2 cut(s) 1468, 1828
BspMI ACCTGC 2 cut(s) 925, 1187
BspOI GCTAGC 1 cut(s) 1127
BspQI GCTCTTC 1 cut(s) 1356
BspT104I TTCGAA 1 cut(s) 1028
BspTNI GGTCTC 1 cut(s) 777
BsrDI GCAATG 1 cut(s) 1299
BsrI ACTGG 1 cut(s) 834
BssECI CCNNGG 5 cut(s) 116, 337, 418, 519, 750
BssNI GRCGYC 1 cut(s) 221
BssT1I CCWWGG 3 cut(s) 116, 337, 519
Bst2UI CCWGG 2 cut(s) 751, 788
Bst4CI ACNGT 7 cut(s) 17, 90, 457, 621, 848, 1532, 1685
Bst6I CTCTTC 3 cut(s) 935, 1356, 1953
BstACI GRCGYC 1 cut(s) 221
BstAPI GCANNNNNTGC 2 cut(s) 331, 1463
BstBI TTCGAA 1 cut(s) 1028
BstC8I GCNNGC 4 cut(s) 54, 1125, 1579, 1698
BstDEI CTNAG 7 cut(s) 179, 315, 584, 655, 663, 1320, 1590
BstDSI CCRYGG 3 cut(s) 337, 418, 519
BstENI CCTNNNNNAGG 1 cut(s) 932
BstF5I GGATG 5 cut(s) 547, 964, 1600, 1758, 1965
BstHHI GCGC 1 cut(s) 336
BstMAI GTCTC 1 cut(s) 777
BstMCI CGRYCG 1 cut(s) 418
BstNI CCWGG 2 cut(s) 751, 788
BstNSI RCATGY 1 cut(s) 1700
BstPAI GACNNNNGTC 1 cut(s) 118
BstSCI CCNGG 2 cut(s) 749, 786
BstSFI CTRYAG 4 cut(s) 143, 1110, 1464, 1824
BstV1I GCAGC 5 cut(s) 334, 967, 1157, 1450, 1840
BstX2I RGATCY 2 cut(s) 968, 1676
BstXI CCANNNNNNTGG 1 cut(s) 1769
BstYI RGATCY 2 cut(s) 968, 1676
BstZI CGGCCG 1 cut(s) 415
BsuI GTATCC 1 cut(s) 848
BsuRI GGCC 5 cut(s) 24, 42, 417, 1011, 1233
BtgI CCRYGG 3 cut(s) 337, 418, 519
BtgZI GCGATG 1 cut(s) 1680
BtsCI GGATG 5 cut(s) 547, 964, 1600, 1758, 1965
BtsI GCAGTG 4 cut(s) 152, 369, 1146, 1186
BtsIMutI CAGTG 6 cut(s) 22, 152, 369, 1146, 1186, 1198
BveI ACCTGC 2 cut(s) 925, 1187
Cac8I GCNNGC 4 cut(s) 54, 1125, 1579, 1698
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 9 cut(s) 23, 41, 113, 120, 272, 545, 914, 925, 1269
Csp6I GTAC 4 cut(s) 30, 427, 844, 1082
CviAII CATG 9 cut(s) 326, 338, 479, 515, 520, 988, 1157, 1697, 1711
CviQI GTAC 4 cut(s) 30, 427, 844, 1082
DdeI CTNAG 7 cut(s) 179, 315, 584, 655, 663, 1320, 1590
DraI TTTAAA 3 cut(s) 12, 1056, 1612
EaeI YGGCCR 1 cut(s) 415
EagI CGGCCG 1 cut(s) 415
Eam1104I CTCTTC 3 cut(s) 935, 1356, 1953
EarI CTCTTC 3 cut(s) 935, 1356, 1953
EclXI CGGCCG 1 cut(s) 415
Eco130I CCWWGG 3 cut(s) 116, 337, 519
Eco31I GGTCTC 1 cut(s) 777
Eco47I GGWCC 7 cut(s) 113, 120, 272, 545, 914, 925, 1269
Eco52I CGGCCG 1 cut(s) 415
Eco57I CTGAAG 2 cut(s) 483, 1742
Eco88I CYCGRG 1 cut(s) 1041
EcoNI CCTNNNNNAGG 1 cut(s) 932
EcoO109I RGGNCCY 1 cut(s) 914
EcoRII CCWGG 2 cut(s) 749, 786
EcoT14I CCWWGG 3 cut(s) 116, 337, 519
EcoT22I ATGCAT 1 cut(s) 1564
ErhI CCWWGG 3 cut(s) 116, 337, 519
FaeI CATG 9 cut(s) 329, 341, 482, 518, 523, 991, 1160, 1700, 1714
FalI AAGNNNNNCTT 4 cut(s) 1068, 1100, 1474, 1506
FaqI GGGAC 2 cut(s) 734, 1443
FatI CATG 9 cut(s) 325, 337, 478, 514, 519, 987, 1156, 1696, 1710
FauNDI CATATG 1 cut(s) 1558
FbaI TGATCA 3 cut(s) 1630, 1641, 1927
FblI GTMKAC 3 cut(s) 850, 1776, 1815
Fnu4HI GCNGC 6 cut(s) 166, 323, 981, 1171, 1464, 1854
FokI GGATG 5 cut(s) 554, 971, 1607, 1745, 1972
Fsp4HI GCNGC 6 cut(s) 166, 323, 981, 1171, 1464, 1854
FspBI CTAG 4 cut(s) 645, 1007, 1124, 1913
GlaI GCGC 1 cut(s) 335
GluI GCNGC 6 cut(s) 166, 323, 981, 1171, 1464, 1854
GsuI CTGGAG 1 cut(s) 809
HaeIII GGCC 5 cut(s) 24, 42, 417, 1011, 1233
HapII CCGG 2 cut(s) 169, 841
HhaI GCGC 1 cut(s) 336
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 9 cut(s) 329, 341, 482, 518, 523, 991, 1160, 1700, 1714
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 1 cut(s) 560
HindII GTYRAC 1 cut(s) 560
HinfI GANTC 8 cut(s) 449, 564, 641, 793, 866, 1707, 1820, 1835
HpaII CCGG 2 cut(s) 169, 841
HphI GGTGA 2 cut(s) 1651, 1844
Hpy166II GTNNAC 5 cut(s) 408, 560, 851, 1777, 1816
Hpy188III TCNNGA 7 cut(s) 181, 539, 645, 1064, 1428, 1730, 1913
Hpy8I GTNNAC 5 cut(s) 408, 560, 851, 1777, 1816
Hpy99I CGWCG 4 cut(s) 94, 223, 226, 1329
HpyAV CCTTC 6 cut(s) 583, 905, 1717, 1777, 1879, 1891
HpyCH4III ACNGT 7 cut(s) 17, 90, 457, 621, 848, 1532, 1685
HpyCH4IV ACGT 2 cut(s) 221, 291
HpyF3I CTNAG 7 cut(s) 179, 315, 584, 655, 663, 1320, 1590
HpySE526I ACGT 2 cut(s) 221, 291
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 9 cut(s) 329, 341, 482, 518, 523, 991, 1160, 1700, 1714
HspAI GCGC 1 cut(s) 334
Ksp22I TGATCA 3 cut(s) 1630, 1641, 1927
LguI GCTCTTC 1 cut(s) 1356
LmnI GCTCC 5 cut(s) 171, 510, 1167, 1717, 1732
Lsp1109I GCAGC 5 cut(s) 334, 967, 1157, 1450, 1840
LweI GCATC 2 cut(s) 309, 1585
MaeI CTAG 4 cut(s) 645, 1007, 1124, 1913
MaeII ACGT 2 cut(s) 221, 291
MaeIII GTNAC 3 cut(s) 150, 457, 1280
MboII GAAGA 4 cut(s) 922, 1373, 1940, 1975
MfeI CAATTG 1 cut(s) 1905
MflI RGATCY 2 cut(s) 968, 1676
MhlI GDGCHC 1 cut(s) 1579
MlyI GAGTC 1 cut(s) 875
Mph1103I ATGCAT 1 cut(s) 1564
MseI TTAA 4 cut(s) 11, 1055, 1479, 1611
MslI CAYNNNNRTG 1 cut(s) 417
MspA1I CMGCKG 2 cut(s) 301, 1403
MspI CCGG 2 cut(s) 169, 841
MspR9I CCNGG 2 cut(s) 751, 788
MunI CAATTG 1 cut(s) 1905
Mva1269I GAATGC 3 cut(s) 1036, 1173, 1676
MvaI CCWGG 2 cut(s) 751, 788
NcoI CCATGG 2 cut(s) 337, 519
NdeI CATATG 1 cut(s) 1558
NheI GCTAGC 1 cut(s) 1123
NlaIII CATG 9 cut(s) 329, 341, 482, 518, 523, 991, 1160, 1700, 1714
NlaIV GGNNCC 3 cut(s) 512, 692, 1728
NmeAIII GCCGAG 1 cut(s) 461
NmuCI GTSAC 1 cut(s) 150
NsiI ATGCAT 1 cut(s) 1564
NspI RCATGY 1 cut(s) 1700
NspV TTCGAA 1 cut(s) 1028
OliI CACNNNNGTG 1 cut(s) 417
PaeI GCATGC 1 cut(s) 1700
PciSI GCTCTTC 1 cut(s) 1356
PctI GAATGC 3 cut(s) 1036, 1173, 1676
PfeI GAWTC 7 cut(s) 449, 564, 641, 793, 1707, 1820, 1835
PflFI GACNNNGTC 1 cut(s) 220
PflMI CCANNNNNTGG 1 cut(s) 520
PkrI GCNGC 6 cut(s) 167, 324, 982, 1172, 1465, 1855
PleI GAGTC 1 cut(s) 874
PpsI GAGTC 1 cut(s) 874
PpuMI RGGWCCY 1 cut(s) 914
PshAI GACNNNNGTC 1 cut(s) 118
Psp1406I AACGTT 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 2 cut(s) 749, 786
PspGI CCWGG 2 cut(s) 749, 786
PspN4I GGNNCC 3 cut(s) 512, 692, 1728
PspPI GGNCC 9 cut(s) 23, 41, 113, 120, 272, 545, 914, 925, 1269
PspPPI RGGWCCY 1 cut(s) 914
PstI CTGCAG 2 cut(s) 1468, 1828
PsuI RGATCY 2 cut(s) 968, 1676
PsyI GACNNNGTC 1 cut(s) 220
PvuII CAGCTG 2 cut(s) 301, 1403
RsaI GTAC 4 cut(s) 31, 428, 845, 1083
RsaNI GTAC 4 cut(s) 30, 427, 844, 1082
RseI CAYNNNNRTG 1 cut(s) 417
SapI GCTCTTC 1 cut(s) 1356
SaqAI TTAA 4 cut(s) 11, 1055, 1479, 1611
SatI GCNGC 6 cut(s) 166, 323, 981, 1171, 1464, 1854
Sau96I GGNCC 9 cut(s) 23, 41, 113, 120, 272, 545, 914, 925, 1269
SchI GAGTC 1 cut(s) 875
ScrFI CCNGG 2 cut(s) 751, 788
SduI GDGCHC 1 cut(s) 1579
SfaNI GCATC 2 cut(s) 309, 1585
SfcI CTRYAG 4 cut(s) 143, 1110, 1464, 1824
SfuI TTCGAA 1 cut(s) 1028
SinI GGWCC 7 cut(s) 113, 120, 272, 545, 914, 925, 1269
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 3 cut(s) 1062, 1799, 1808
SmoI CTYRAG 3 cut(s) 1062, 1799, 1808
SphI GCATGC 1 cut(s) 1700
SsiI CCGC 4 cut(s) 166, 433, 755, 1372
SspMI CTAG 4 cut(s) 645, 1007, 1124, 1913
StyD4I CCNGG 2 cut(s) 749, 786
StyI CCWWGG 3 cut(s) 116, 337, 519
TaaI ACNGT 7 cut(s) 17, 90, 457, 621, 848, 1532, 1685
TaiI ACGT 2 cut(s) 224, 294
TaqI TCGA 4 cut(s) 92, 237, 712, 1028
TaqII GACCGA 1 cut(s) 260
TatI WGTACW 3 cut(s) 29, 426, 1081
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 7 cut(s) 449, 564, 641, 793, 1707, 1820, 1835
Tru1I TTAA 4 cut(s) 11, 1055, 1479, 1611
Tru9I TTAA 4 cut(s) 11, 1055, 1479, 1611
TscAI CASTG 6 cut(s) 22, 159, 376, 1153, 1186, 1198
TseFI GTSAC 1 cut(s) 150
TseI GCWGC 5 cut(s) 322, 980, 1170, 1463, 1853
Tsp45I GTSAC 1 cut(s) 150
TspDTI ATGAA 6 cut(s) 698, 989, 1080, 1683, 1972, 1976
TspGWI ACGGA 2 cut(s) 126, 1282
TspRI CASTG 6 cut(s) 22, 159, 376, 1153, 1186, 1198
Tth111I GACNNNGTC 1 cut(s) 220
Van91I CCANNNNNTGG 1 cut(s) 520
VpaK11BI GGWCC 7 cut(s) 113, 120, 272, 545, 914, 925, 1269
XagI CCTNNNNNAGG 1 cut(s) 932
XapI RAATTY 6 cut(s) 94, 278, 995, 1140, 1607, 1790
XbaI TCTAGA 2 cut(s) 644, 1912
XceI RCATGY 1 cut(s) 1700
XmiI GTMKAC 3 cut(s) 850, 1776, 1815
XspI CTAG 4 cut(s) 645, 1007, 1124, 1913
ZraI GACGTC 1 cut(s) 222
Zsp2I ATGCAT 1 cut(s) 1564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.