Rh7DG100300

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0006889
Physical Location & Seq
Forward (+)
8338965 .. 8344655
5691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG100300.1

Sequence Viewer

Length: 1704 bp
ATGGGGAAAGAGAACCAGACGACGTCGGCTATGGAGATCGACAATCAAAAGGCGAACTCCTCCGATCAGATCGGTCCTAAATTCTCAATCAACGTTTTACAGCTGTTGAAATCTGCTCAGATGCAGCATGGATTGCGCCATGGGGATTACACTCGTTATCGGAGGTATTGCACTGCAAGGTTGAGGAGGCTGTATAAGTCATTGAAGTTCACACACGGCCGTGGCAAGTACAACCGCCGAGCAATAACCGAATCAACTGTTACTGAAGTGAGGTTTCTCCATGTGGTTCTATATACGGCAGAGAGGGCTTGGAGCCATGCCATGGAAAAAAGGCAAGTTCTGGATGGTCCAAATGCTCGTCAACGAATCTATCTGATTGGTAGGCTGAGGAAGGCAGTAAAATGGGCTACTCTTTTTGCACAGTTGTCTGCAATCAAGGGAGATTCTAGAACTTCCTTAGAAGCTGAGGCTTATGCCTCTTTTATGAAAGGGAACCTGTTGTTTGAACAAGATCGAAATTGGGACACAGCATTACTGAACTTCAAAAGTGCCAGGGCGGTTTATGAGGAGCTTGCGAAATATGGAGACCTGGAGAATCAAGTTTTATGCCGTGAGCGTGTTGAGGAATTAGAACCTAGTATCCGGTACTGTCTACACAAGATTGGAGAGTCAAATCTACAAGGCTCTGAACTTCTACATATTGGCGAGATGGAAGGACCTGCTTTGGACCTCTTCAAGGCTAAATTGGAGGCTGTTATGGATGAGGCAAGATCTCAACAGGCTGCCTCCATGACAGAATTTCATTGGCTAGGCCATAGATTTCCAATTTCGAATGCAAAAACTCGGGTTTCTATTTTAAAAGCTCAAGACTTGGAGAAAGATGTACTTGGTTCATCAGCTAATTCACTCCCTACAGAGAAAAGGCTAGCCATTTTTGACAAAATTTTCACTGCATACCATGAGGCAAGGAGCTGCATTCGCAGTGATTTGGTCAGTGCAGGTAGTTCTGAAAATGTTAAAGATGACTTGAATGGCCTCGATAAAGCTGTTAGTGCTGTATTAGGACAACGGACCATTGAGCGTAACCTACTTTTGGTTAGCATTGCAAAGAGCAAACTCACTAAGCGACGAGATGACAAAAATGAGAGAACTACGAAGCCAGAAGAGCTTGTCCGCTTGTATGATCTCCTATTACAGAATACAGCTGACCTTTCAGATTTAGTCAGTTCGGGACGAGAAAAAACATCAGAGGAAGTCACATTTTCTGCAGAATGTGAGCTTAAAAGTTTGGCTTTCCGAGCAGAAAGGTGTTTCTTTTTGGGTAGATCATACAGTTTGGCTGGTAAGAGGGTGGAAGCATATTCATTGTATTGCCGTGCTCGCACACTTGCTGAGGATGCCCTGCAAAAATTTAAAGCTGTTGATAATGCTGATCAGGTGGTGATCAAAGAGTTGAAGCTATTGTGCGATGAATGCAGATCTAACAGTTGTATAGAGCATGCAACAGGAATCATGGAGGAGCTGAAGGCTCCTGAAAATCTCTCACAACAGATTTCTAACATCCATATATCTGGAGTTGACACGAAGGTATGCATTATGCTTTTCCTCTTCATCAGGAAATATTTGAATGATGTCATGAACCTCCATAGTTTAGTAGCACTGGCCAAGGAGATTTTGTGTCCTGGTAATCATTCTTCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
SRP68

Protein Analysis

567

Amino Acids

64.28

Weight (kDa)

8.87

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP68 PF16969 45 - 519 3.9e-142 RNA-binding signal recognition particle 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
Acc36I ACCTGC 2 cut(s) 727, 989
AccB7I CCANNNNNTGG 1 cut(s) 322
AccI GTMKAC 1 cut(s) 652
AciI CCGC 3 cut(s) 235, 557, 1174
AclI AACGTT 1 cut(s) 93
AcoI YGGCCR 2 cut(s) 217, 1662
AcsI RAATTY 4 cut(s) 80, 797, 942, 1409
AcuI CTGAAG 2 cut(s) 285, 1544
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 3 cut(s) 230, 647, 885
AfiI CCNNNNNNNGG 3 cut(s) 322, 736, 1093
AgsI TTSAA 9 cut(s) 109, 205, 506, 544, 736, 1030, 1456, 1627, 1698
AjnI CCWGG 3 cut(s) 551, 588, 1681
AleI CACNNNNGTG 1 cut(s) 219
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
Alw21I GWGCWC 1 cut(s) 1381
Alw26I GTCTC 1 cut(s) 579
Ama87I CYCGRG 1 cut(s) 843
AoxI GGCC 4 cut(s) 217, 811, 1033, 1662
ApeKI GCWGC 3 cut(s) 124, 782, 972
ApoI RAATTY 4 cut(s) 80, 797, 942, 1409
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 5 cut(s) 74, 347, 716, 727, 1071
AsuHPI GGTGA 1 cut(s) 1453
AsuII TTCGAA 1 cut(s) 830
AsuNHI GCTAGC 1 cut(s) 925
AvaI CYCGRG 1 cut(s) 843
AvaII GGWCC 5 cut(s) 74, 347, 716, 727, 1071
BalI TGGCCA 1 cut(s) 1664
BarI GAAGNNNNNNTAC 1 cut(s) 1678
Bbv12I GWGCWC 1 cut(s) 1381
BbvCI CCTCAGC 3 cut(s) 386, 465, 1392
BbvI GCAGC 3 cut(s) 136, 769, 959
BccI CCATC 2 cut(s) 338, 703
BceAI ACGGC 5 cut(s) 204, 232, 312, 594, 1359
BciT130I CCWGG 3 cut(s) 553, 590, 1683
BciVI GTATCC 1 cut(s) 650
BclI TGATCA 2 cut(s) 1432, 1443
BcoDI GTCTC 1 cut(s) 579
BfaI CTAG 4 cut(s) 447, 636, 809, 926
BfmI CTRYAG 2 cut(s) 912, 1266
BfuAI ACCTGC 2 cut(s) 727, 989
BfuI GTATCC 1 cut(s) 650
BglII AGATCT 2 cut(s) 770, 1478
BisI GCNGC 3 cut(s) 125, 783, 973
BlsI GCNGC 3 cut(s) 126, 784, 974
Bme1390I CCNGG 3 cut(s) 553, 590, 1683
Bme18I GGWCC 5 cut(s) 74, 347, 716, 727, 1071
BmeT110I CYCGRG 1 cut(s) 843
BmgT120I GGNCC 5 cut(s) 74, 347, 716, 727, 1071
BmiI GGNNCC 3 cut(s) 314, 494, 1530
BmrFI CCNGG 3 cut(s) 553, 590, 1683
BmsI GCATC 2 cut(s) 111, 1387
BmtI GCTAGC 1 cut(s) 929
BpmI CTGGAG 2 cut(s) 611, 1593
Bpu10I CCTNAGC 3 cut(s) 386, 465, 1392
Bpu14I TTCGAA 1 cut(s) 830
BpuEI CTTGAG 1 cut(s) 849
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 579
BsaJI CCNNGG 5 cut(s) 139, 220, 321, 552, 1665
BsaWI WCCGGW 1 cut(s) 642
BsaXI ACNNNNNCTCC 2 cut(s) 1661, 1691
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 736, 1093
Bse1I ACTGG 1 cut(s) 1665
Bse3DI GCAATG 1 cut(s) 1101
BseBI CCWGG 3 cut(s) 553, 590, 1683
BseDI CCNNGG 5 cut(s) 139, 220, 321, 552, 1665
BseGI GGATG 4 cut(s) 349, 766, 1402, 1560
BseLI CCNNNNNNNGG 3 cut(s) 322, 736, 1093
BseMI GCAATG 1 cut(s) 1101
BseMII CTCAG 4 cut(s) 131, 377, 456, 1383
BseNI ACTGG 1 cut(s) 1665
BseRI GAGGAG 4 cut(s) 49, 199, 581, 1532
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 3 cut(s) 136, 769, 959
BsgI GTGCAG 1 cut(s) 1017
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 4 cut(s) 219, 813, 1035, 1664
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 1381
BsiHKCI CYCGRG 1 cut(s) 843
BsiSI CCGG 1 cut(s) 643
BslFI GGGAC 2 cut(s) 536, 1245
BslI CCNNNNNNNGG 3 cut(s) 322, 736, 1093
BsmAI GTCTC 1 cut(s) 579
BsmFI GGGAC 2 cut(s) 536, 1245
BsmI GAATGC 3 cut(s) 838, 975, 1478
BsnI GGCC 4 cut(s) 219, 813, 1035, 1664
Bso31I GGTCTC 1 cut(s) 579
BsoBI CYCGRG 1 cut(s) 843
Bsp119I TTCGAA 1 cut(s) 830
Bsp1286I GDGCHC 1 cut(s) 1381
Bsp19I CCATGG 2 cut(s) 139, 321
BspACI CCGC 3 cut(s) 235, 557, 1174
BspANI GGCC 4 cut(s) 219, 813, 1035, 1664
BspCNI CTCAG 4 cut(s) 130, 378, 457, 1384
BspHI TCATGA 1 cut(s) 1635
BspLI GGNNCC 3 cut(s) 314, 494, 1530
BspMAI CTGCAG 1 cut(s) 1270
BspMI ACCTGC 2 cut(s) 727, 989
BspOI GCTAGC 1 cut(s) 929
BspQI GCTCTTC 1 cut(s) 1158
BspT104I TTCGAA 1 cut(s) 830
BspTNI GGTCTC 1 cut(s) 579
BsrDI GCAATG 1 cut(s) 1101
BsrI ACTGG 1 cut(s) 1665
BssECI CCNNGG 5 cut(s) 139, 220, 321, 552, 1665
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 3 cut(s) 139, 321, 1665
Bst2UI CCWGG 3 cut(s) 553, 590, 1683
Bst4CI ACNGT 5 cut(s) 259, 423, 650, 1334, 1487
Bst6I CTCTTC 3 cut(s) 737, 1158, 1613
BstACI GRCGYC 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 133
BstBI TTCGAA 1 cut(s) 830
BstC8I GCNNGC 4 cut(s) 573, 927, 1381, 1500
BstDEI CTNAG 6 cut(s) 117, 386, 457, 465, 1122, 1392
BstDSI CCRYGG 3 cut(s) 139, 220, 321
BstENI CCTNNNNNAGG 1 cut(s) 734
BstF5I GGATG 4 cut(s) 349, 766, 1402, 1560
BstHHI GCGC 1 cut(s) 138
BstMAI GTCTC 1 cut(s) 579
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 9 cut(s) 133, 305, 978, 1052, 1165, 1298, 1380, 1397, 1473
BstNI CCWGG 3 cut(s) 553, 590, 1683
BstNSI RCATGY 1 cut(s) 1502
BstSCI CCNGG 3 cut(s) 551, 588, 1681
BstSFI CTRYAG 2 cut(s) 912, 1266
BstV1I GCAGC 3 cut(s) 136, 769, 959
BstX2I RGATCY 2 cut(s) 770, 1478
BstXI CCANNNNNNTGG 1 cut(s) 1571
BstYI RGATCY 2 cut(s) 770, 1478
BstZI CGGCCG 1 cut(s) 217
BsuI GTATCC 1 cut(s) 650
BsuRI GGCC 4 cut(s) 219, 813, 1035, 1664
BtgI CCRYGG 3 cut(s) 139, 220, 321
BtgZI GCGATG 1 cut(s) 1482
BtsCI GGATG 4 cut(s) 349, 766, 1402, 1560
BtsI GCAGTG 3 cut(s) 171, 948, 988
BtsIMutI CAGTG 5 cut(s) 171, 948, 988, 1000, 1658
BveI ACCTGC 2 cut(s) 727, 989
Cac8I GCNNGC 4 cut(s) 573, 927, 1381, 1500
CciI TCATGA 1 cut(s) 1635
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 5 cut(s) 74, 347, 716, 727, 1071
Csp6I GTAC 3 cut(s) 229, 646, 884
CviQI GTAC 3 cut(s) 229, 646, 884
DdeI CTNAG 6 cut(s) 117, 386, 457, 465, 1122, 1392
DraI TTTAAA 2 cut(s) 858, 1414
EaeI YGGCCR 2 cut(s) 217, 1662
EagI CGGCCG 1 cut(s) 217
Eam1104I CTCTTC 3 cut(s) 737, 1158, 1613
EarI CTCTTC 3 cut(s) 737, 1158, 1613
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 3 cut(s) 139, 321, 1665
Eco31I GGTCTC 1 cut(s) 579
Eco47I GGWCC 5 cut(s) 74, 347, 716, 727, 1071
Eco52I CGGCCG 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 285, 1544
Eco88I CYCGRG 1 cut(s) 843
EcoNI CCTNNNNNAGG 1 cut(s) 734
EcoO109I RGGNCCY 1 cut(s) 716
EcoRII CCWGG 3 cut(s) 551, 588, 1681
EcoT14I CCWWGG 3 cut(s) 139, 321, 1665
EcoT22I ATGCAT 1 cut(s) 1595
ErhI CCWWGG 3 cut(s) 139, 321, 1665
FalI AAGNNNNNCTT 4 cut(s) 870, 902, 1276, 1308
FaqI GGGAC 2 cut(s) 536, 1245
FbaI TGATCA 2 cut(s) 1432, 1443
FblI GTMKAC 1 cut(s) 652
Fnu4HI GCNGC 3 cut(s) 125, 783, 973
FokI GGATG 4 cut(s) 356, 773, 1409, 1547
Fsp4HI GCNGC 3 cut(s) 125, 783, 973
FspBI CTAG 4 cut(s) 447, 636, 809, 926
GlaI GCGC 1 cut(s) 137
GluI GCNGC 3 cut(s) 125, 783, 973
GsuI CTGGAG 2 cut(s) 611, 1593
HaeIII GGCC 4 cut(s) 219, 813, 1035, 1664
HapII CCGG 1 cut(s) 643
HhaI GCGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 23
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HincII GTYRAC 2 cut(s) 362, 1579
HindII GTYRAC 2 cut(s) 362, 1579
HinfI GANTC 6 cut(s) 251, 366, 443, 595, 668, 1509
HpaII CCGG 1 cut(s) 643
HphI GGTGA 1 cut(s) 1453
Hpy166II GTNNAC 4 cut(s) 210, 362, 653, 1579
Hpy188III TCNNGA 8 cut(s) 341, 447, 866, 1230, 1532, 1572, 1615, 1636
Hpy8I GTNNAC 4 cut(s) 210, 362, 653, 1579
Hpy99I CGWCG 3 cut(s) 25, 28, 1131
HpyAV CCTTC 4 cut(s) 385, 707, 1519, 1579
HpyCH4III ACNGT 5 cut(s) 259, 423, 650, 1334, 1487
HpyCH4IV ACGT 2 cut(s) 23, 93
HpyF10VI GCNNNNNNNGC 9 cut(s) 133, 305, 978, 1052, 1165, 1298, 1380, 1397, 1473
HpyF3I CTNAG 6 cut(s) 117, 386, 457, 465, 1122, 1392
HpySE526I ACGT 2 cut(s) 23, 93
Hsp92I GRCGYC 1 cut(s) 23
HspAI GCGC 1 cut(s) 136
Ksp22I TGATCA 2 cut(s) 1432, 1443
LguI GCTCTTC 1 cut(s) 1158
LmnI GCTCC 5 cut(s) 312, 568, 969, 1519, 1534
Lsp1109I GCAGC 3 cut(s) 136, 769, 959
LweI GCATC 2 cut(s) 111, 1387
MaeI CTAG 4 cut(s) 447, 636, 809, 926
MaeII ACGT 2 cut(s) 23, 93
MaeIII GTNAC 3 cut(s) 259, 1082, 1255
MboII GAAGA 4 cut(s) 724, 1175, 1600, 1686
MflI RGATCY 2 cut(s) 770, 1478
MhlI GDGCHC 1 cut(s) 1381
MlsI TGGCCA 1 cut(s) 1664
MluNI TGGCCA 1 cut(s) 1664
MlyI GAGTC 1 cut(s) 677
Mox20I TGGCCA 1 cut(s) 1664
Mph1103I ATGCAT 1 cut(s) 1595
MscI TGGCCA 1 cut(s) 1664
MseI TTAA 4 cut(s) 857, 1017, 1281, 1413
MslI CAYNNNNRTG 1 cut(s) 219
Msp20I TGGCCA 1 cut(s) 1664
MspA1I CMGCKG 2 cut(s) 103, 1205
MspI CCGG 1 cut(s) 643
MspR9I CCNGG 3 cut(s) 553, 590, 1683
Mva1269I GAATGC 3 cut(s) 838, 975, 1478
MvaI CCWGG 3 cut(s) 553, 590, 1683
MwoI GCNNNNNNNGC 9 cut(s) 133, 305, 978, 1052, 1165, 1298, 1380, 1397, 1473
NcoI CCATGG 2 cut(s) 139, 321
NheI GCTAGC 1 cut(s) 925
NlaIV GGNNCC 3 cut(s) 314, 494, 1530
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 1255
NsiI ATGCAT 1 cut(s) 1595
NspI RCATGY 1 cut(s) 1502
NspV TTCGAA 1 cut(s) 830
OliI CACNNNNGTG 1 cut(s) 219
PaeI GCATGC 1 cut(s) 1502
PagI TCATGA 1 cut(s) 1635
PciSI GCTCTTC 1 cut(s) 1158
PctI GAATGC 3 cut(s) 838, 975, 1478
PfeI GAWTC 5 cut(s) 251, 366, 443, 595, 1509
PflFI GACNNNGTC 1 cut(s) 22
PflMI CCANNNNNTGG 1 cut(s) 322
PkrI GCNGC 3 cut(s) 126, 784, 974
PleI GAGTC 1 cut(s) 676
PpsI GAGTC 1 cut(s) 676
PpuMI RGGWCCY 1 cut(s) 716
Psp1406I AACGTT 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 716
Psp6I CCWGG 3 cut(s) 551, 588, 1681
PspGI CCWGG 3 cut(s) 551, 588, 1681
PspN4I GGNNCC 3 cut(s) 314, 494, 1530
PspPI GGNCC 5 cut(s) 74, 347, 716, 727, 1071
PspPPI RGGWCCY 1 cut(s) 716
PstI CTGCAG 1 cut(s) 1270
PsuI RGATCY 2 cut(s) 770, 1478
PsyI GACNNNGTC 1 cut(s) 22
PvuII CAGCTG 2 cut(s) 103, 1205
RsaI GTAC 3 cut(s) 230, 647, 885
RsaNI GTAC 3 cut(s) 229, 646, 884
RseI CAYNNNNRTG 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 1158
SaqAI TTAA 4 cut(s) 857, 1017, 1281, 1413
SatI GCNGC 3 cut(s) 125, 783, 973
Sau96I GGNCC 5 cut(s) 74, 347, 716, 727, 1071
SchI GAGTC 1 cut(s) 677
ScrFI CCNGG 3 cut(s) 553, 590, 1683
SduI GDGCHC 1 cut(s) 1381
SfaNI GCATC 2 cut(s) 111, 1387
SfcI CTRYAG 2 cut(s) 912, 1266
SfuI TTCGAA 1 cut(s) 830
SinI GGWCC 5 cut(s) 74, 347, 716, 727, 1071
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 864
SmoI CTYRAG 1 cut(s) 864
SphI GCATGC 1 cut(s) 1502
SsiI CCGC 3 cut(s) 235, 557, 1174
SspI AATATT 1 cut(s) 1622
SspMI CTAG 4 cut(s) 447, 636, 809, 926
StyD4I CCNGG 3 cut(s) 551, 588, 1681
StyI CCWWGG 3 cut(s) 139, 321, 1665
TaaI ACNGT 5 cut(s) 259, 423, 650, 1334, 1487
TaiI ACGT 2 cut(s) 26, 96
TaqI TCGA 4 cut(s) 39, 514, 830, 1038
TaqII GACCGA 1 cut(s) 62
TatI WGTACW 2 cut(s) 228, 883
TfiI GAWTC 5 cut(s) 251, 366, 443, 595, 1509
Tru1I TTAA 4 cut(s) 857, 1017, 1281, 1413
Tru9I TTAA 4 cut(s) 857, 1017, 1281, 1413
TscAI CASTG 5 cut(s) 178, 955, 988, 1000, 1665
TseFI GTSAC 1 cut(s) 1255
TseI GCWGC 3 cut(s) 124, 782, 972
Tsp45I GTSAC 1 cut(s) 1255
TspDTI ATGAA 7 cut(s) 500, 791, 882, 1353, 1485, 1600, 1652
TspGWI ACGGA 1 cut(s) 1084
TspRI CASTG 5 cut(s) 178, 955, 988, 1000, 1665
Tth111I GACNNNGTC 1 cut(s) 22
Van91I CCANNNNNTGG 1 cut(s) 322
VpaK11BI GGWCC 5 cut(s) 74, 347, 716, 727, 1071
XagI CCTNNNNNAGG 1 cut(s) 734
XapI RAATTY 4 cut(s) 80, 797, 942, 1409
XbaI TCTAGA 1 cut(s) 446
XceI RCATGY 1 cut(s) 1502
XmiI GTMKAC 1 cut(s) 652
XspI CTAG 4 cut(s) 447, 636, 809, 926
ZraI GACGTC 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 1595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.