Rh7CG101800

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006889
Physical Location & Seq
Forward (+)
7644016 .. 7649558
5543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG101800.1

Sequence Viewer

Length: 1359 bp
ATGGGGAAAGAGAACCAGAACCAGACGACGTCGGCTATGGAGATCGACAATCAAAAGGCGAACACCTCCGATCAGATCGGTCCTAAATTCTCAATCAACGTTTTACAGCTGTTGAAATCTGCTCAGATGCAGCATGGATTGCGCCATGGGGATTACACTCGTTATCGGAGGTATTGCACTGCAAGGTTGAGGAGGCTGTATAAGTCATTGAAGTTCACACACGGCCGTGGCAAGTACAACCGCCGAACAATAACCGAATCAACTGTTACTGAAGTGAGGTTTCTCCATGTGGTTCTATATACGGCAGAGAGGGCTTGGAGCCATGCCATGGAAAAAAGGCAAGTTCTGGATGGTCCAAATGCTCGTCAACGAATCTATCTGATTGGTAGGCTGAGGAAGGCAGTAAAATGGGCTACTCTTTTTGCACAGTTGTCTGCAATCAAGGGAGATTCTAGAACTTCCTTAGAAGCTGAGGCTTATGCCTCTTTTATGAAAGGGAACCTGTTGTTTGAACAAGATCGAAATTGGGACACAGCATTACTGAACTTCAAAAGTGCCAGGGCGGTTTATGAGGAACTTGCGAAATATGGAGACCTGGAGAATCAAGTTTTATGCCGTGAGCGTGTTGAGGAATTAGAACCTAGTATCCGGTACTGTCTACACAAGATTGGAGAGTCAAATCTACAAGGCTCTGAACTTCTACATATTGGCGAGATGGAAGGACCTGCTTTGGACCTCTTCAAGGCTAAATTGGAGGCTGTTATGGATGAGGCAAGATCTCAACAGGCTGCCTCCATGACAGAATTTCATTGGCTAGGCCATAGATTTCCAATTTCGAATGCAAAAACTCGGGTTTCTATTTTAAAAGCTCAAGACTTGGAGAAAGATGTACTTGGTTCATCAGCTAATTCACTCCCTACAGAGAAAAGGCTAGCCATTTTTGACAAAATTTTCACTGCATACCATGAGGCAAGGAGCTGCATTCGCAGTGATTTGGTCAGTGCAGGTAGTTCTGAAAATGTCAAAGATGAATTGAATGGCCTTGATAAAGCTGTTAGTGCTGTATTAGGACAACGGACCATTGAGCGTAACCTACTTTTGGTTAGCATTGCAAAGAGCAAACTCACTAAGCGACGAGATGACAAAAATGAGAGAACTACGAAGCCAGAAGAGCTTGTGCGCTTGTATGATCTTCTATTACAGAATACAGCTGACCTTTCAGATTTAGTCAGTTCGGGACGAGAAAAAACATCAGAGGAAGTCACATTTGCTGCAGAATGTGAGCTTAAAAGTTTGGCTTTCCGAGCAGAAAGGTTTTTTCTCCTTTTTTTATTTATTTATAGACCCTTTAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
SRP68

Protein Analysis

452

Amino Acids

51.71

Weight (kDa)

9.3

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP68 PF16969 47 - 441 4.8e-121 RNA-binding signal recognition particle 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 32
Acc36I ACCTGC 2 cut(s) 733, 995
AccB7I CCANNNNNTGG 1 cut(s) 328
AccI GTMKAC 1 cut(s) 658
AciI CCGC 2 cut(s) 241, 563
AclI AACGTT 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 3 cut(s) 86, 803, 948
AcuI CTGAAG 1 cut(s) 291
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 3 cut(s) 236, 653, 891
AfiI CCNNNNNNNGG 3 cut(s) 328, 742, 1099
AgsI TTSAA 6 cut(s) 115, 211, 512, 550, 742, 1036
AjnI CCWGG 2 cut(s) 557, 594
AleI CACNNNNGTG 1 cut(s) 225
AloI GAACNNNNNNTCC 2 cut(s) 630, 662
Alw26I GTCTC 1 cut(s) 585
Ama87I CYCGRG 1 cut(s) 849
AoxI GGCC 3 cut(s) 223, 817, 1039
ApeKI GCWGC 4 cut(s) 130, 788, 978, 1271
ApoI RAATTY 3 cut(s) 86, 803, 948
AspLEI GCGC 2 cut(s) 144, 1182
AspS9I GGNCC 5 cut(s) 80, 353, 722, 733, 1077
AsuII TTCGAA 1 cut(s) 836
AsuNHI GCTAGC 1 cut(s) 931
AvaI CYCGRG 1 cut(s) 849
AvaII GGWCC 5 cut(s) 80, 353, 722, 733, 1077
BbvCI CCTCAGC 2 cut(s) 392, 471
BbvI GCAGC 4 cut(s) 142, 775, 965, 1258
BccI CCATC 2 cut(s) 344, 709
BceAI ACGGC 4 cut(s) 210, 238, 318, 600
BciT130I CCWGG 2 cut(s) 559, 596
BciVI GTATCC 1 cut(s) 656
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 4 cut(s) 453, 642, 815, 932
BfmI CTRYAG 2 cut(s) 918, 1272
BfuAI ACCTGC 2 cut(s) 733, 995
BfuI GTATCC 1 cut(s) 656
BglII AGATCT 1 cut(s) 776
BisI GCNGC 4 cut(s) 131, 789, 979, 1272
BlsI GCNGC 4 cut(s) 132, 790, 980, 1273
Bme1390I CCNGG 2 cut(s) 559, 596
Bme18I GGWCC 5 cut(s) 80, 353, 722, 733, 1077
BmeT110I CYCGRG 1 cut(s) 849
BmgT120I GGNCC 5 cut(s) 80, 353, 722, 733, 1077
BmiI GGNNCC 2 cut(s) 320, 500
BmrFI CCNGG 2 cut(s) 559, 596
BmsI GCATC 1 cut(s) 117
BmtI GCTAGC 1 cut(s) 935
BpmI CTGGAG 1 cut(s) 617
Bpu10I CCTNAGC 2 cut(s) 392, 471
Bpu14I TTCGAA 1 cut(s) 836
BpuEI CTTGAG 1 cut(s) 855
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 585
BsaJI CCNNGG 4 cut(s) 145, 226, 327, 558
BsaWI WCCGGW 1 cut(s) 648
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 742, 1099
Bse3DI GCAATG 1 cut(s) 1107
BseBI CCWGG 2 cut(s) 559, 596
BseDI CCNNGG 4 cut(s) 145, 226, 327, 558
BseGI GGATG 2 cut(s) 355, 772
BseLI CCNNNNNNNGG 3 cut(s) 328, 742, 1099
BseMI GCAATG 1 cut(s) 1107
BseMII CTCAG 3 cut(s) 137, 383, 462
BseRI GAGGAG 1 cut(s) 205
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 4 cut(s) 142, 775, 965, 1258
BsgI GTGCAG 1 cut(s) 1023
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 3 cut(s) 225, 819, 1041
BsiEI CGRYCG 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 849
BsiSI CCGG 1 cut(s) 649
BslFI GGGAC 2 cut(s) 542, 1251
BslI CCNNNNNNNGG 3 cut(s) 328, 742, 1099
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 2 cut(s) 542, 1251
BsmI GAATGC 2 cut(s) 844, 981
BsnI GGCC 3 cut(s) 225, 819, 1041
Bso31I GGTCTC 1 cut(s) 585
BsoBI CYCGRG 1 cut(s) 849
Bsp119I TTCGAA 1 cut(s) 836
Bsp143I GATC 6 cut(s) 42, 70, 75, 517, 776, 1189
Bsp19I CCATGG 2 cut(s) 145, 327
BspACI CCGC 2 cut(s) 241, 563
BspANI GGCC 3 cut(s) 225, 819, 1041
BspCNI CTCAG 3 cut(s) 136, 384, 463
BspLI GGNNCC 2 cut(s) 320, 500
BspMAI CTGCAG 1 cut(s) 1276
BspMI ACCTGC 2 cut(s) 733, 995
BspOI GCTAGC 1 cut(s) 935
BspQI GCTCTTC 1 cut(s) 1164
BspT104I TTCGAA 1 cut(s) 836
BspTNI GGTCTC 1 cut(s) 585
BsrDI GCAATG 1 cut(s) 1107
BssECI CCNNGG 4 cut(s) 145, 226, 327, 558
BssMI GATC 6 cut(s) 42, 70, 75, 517, 776, 1189
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 2 cut(s) 145, 327
Bst2UI CCWGG 2 cut(s) 559, 596
Bst4CI ACNGT 3 cut(s) 265, 429, 656
Bst6I CTCTTC 2 cut(s) 743, 1164
BstACI GRCGYC 1 cut(s) 29
BstAPI GCANNNNNTGC 1 cut(s) 139
BstBI TTCGAA 1 cut(s) 836
BstC8I GCNNGC 1 cut(s) 933
BstDEI CTNAG 5 cut(s) 123, 392, 463, 471, 1128
BstDSI CCRYGG 3 cut(s) 145, 226, 327
BstENI CCTNNNNNAGG 1 cut(s) 740
BstF5I GGATG 2 cut(s) 355, 772
BstHHI GCGC 2 cut(s) 144, 1182
BstKTI GATC 6 cut(s) 45, 73, 78, 520, 779, 1192
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 6 cut(s) 42, 70, 75, 517, 776, 1189
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 6 cut(s) 139, 311, 984, 1058, 1171, 1304
BstNI CCWGG 2 cut(s) 559, 596
BstSCI CCNGG 2 cut(s) 557, 594
BstSFI CTRYAG 2 cut(s) 918, 1272
BstV1I GCAGC 4 cut(s) 142, 775, 965, 1258
BstX2I RGATCY 1 cut(s) 776
BstYI RGATCY 1 cut(s) 776
BstZI CGGCCG 1 cut(s) 223
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 3 cut(s) 225, 819, 1041
BtgI CCRYGG 3 cut(s) 145, 226, 327
BtsCI GGATG 2 cut(s) 355, 772
BtsI GCAGTG 3 cut(s) 177, 954, 994
BtsIMutI CAGTG 4 cut(s) 177, 954, 994, 1006
BveI ACCTGC 2 cut(s) 733, 995
Cac8I GCNNGC 1 cut(s) 933
CfoI GCGC 2 cut(s) 144, 1182
Cfr13I GGNCC 5 cut(s) 80, 353, 722, 733, 1077
Csp6I GTAC 3 cut(s) 235, 652, 890
CviAII CATG 7 cut(s) 134, 146, 287, 323, 328, 796, 965
CviQI GTAC 3 cut(s) 235, 652, 890
DdeI CTNAG 5 cut(s) 123, 392, 463, 471, 1128
DpnI GATC 6 cut(s) 44, 72, 77, 519, 778, 1191
DpnII GATC 6 cut(s) 42, 70, 75, 517, 776, 1189
DraI TTTAAA 1 cut(s) 864
EaeI YGGCCR 1 cut(s) 223
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 743, 1164
EarI CTCTTC 2 cut(s) 743, 1164
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 145, 327
Eco31I GGTCTC 1 cut(s) 585
Eco47I GGWCC 5 cut(s) 80, 353, 722, 733, 1077
Eco52I CGGCCG 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 849
EcoNI CCTNNNNNAGG 1 cut(s) 740
EcoO109I RGGNCCY 1 cut(s) 722
EcoRII CCWGG 2 cut(s) 557, 594
EcoT14I CCWWGG 2 cut(s) 145, 327
ErhI CCWWGG 2 cut(s) 145, 327
FaeI CATG 7 cut(s) 137, 149, 290, 326, 331, 799, 968
FalI AAGNNNNNCTT 4 cut(s) 876, 908, 1282, 1314
FaqI GGGAC 2 cut(s) 542, 1251
FatI CATG 7 cut(s) 133, 145, 286, 322, 327, 795, 964
FblI GTMKAC 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 131, 789, 979, 1272
FokI GGATG 2 cut(s) 362, 779
Fsp4HI GCNGC 4 cut(s) 131, 789, 979, 1272
FspBI CTAG 4 cut(s) 453, 642, 815, 932
GlaI GCGC 2 cut(s) 143, 1181
GluI GCNGC 4 cut(s) 131, 789, 979, 1272
GsuI CTGGAG 1 cut(s) 617
HaeIII GGCC 3 cut(s) 225, 819, 1041
HapII CCGG 1 cut(s) 649
HhaI GCGC 2 cut(s) 144, 1182
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 7 cut(s) 137, 149, 290, 326, 331, 799, 968
Hin6I GCGC 2 cut(s) 142, 1180
HinP1I GCGC 2 cut(s) 142, 1180
HincII GTYRAC 1 cut(s) 368
HindII GTYRAC 1 cut(s) 368
HinfI GANTC 5 cut(s) 257, 372, 449, 601, 674
HpaII CCGG 1 cut(s) 649
Hpy166II GTNNAC 3 cut(s) 216, 368, 659
Hpy188III TCNNGA 4 cut(s) 347, 453, 872, 1236
Hpy8I GTNNAC 3 cut(s) 216, 368, 659
Hpy99I CGWCG 3 cut(s) 31, 34, 1137
HpyAV CCTTC 2 cut(s) 391, 713
HpyCH4III ACNGT 3 cut(s) 265, 429, 656
HpyCH4IV ACGT 2 cut(s) 29, 99
HpyF10VI GCNNNNNNNGC 6 cut(s) 139, 311, 984, 1058, 1171, 1304
HpyF3I CTNAG 5 cut(s) 123, 392, 463, 471, 1128
HpySE526I ACGT 2 cut(s) 29, 99
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 7 cut(s) 137, 149, 290, 326, 331, 799, 968
HspAI GCGC 2 cut(s) 142, 1180
Kzo9I GATC 6 cut(s) 42, 70, 75, 517, 776, 1189
LguI GCTCTTC 1 cut(s) 1164
LmnI GCTCC 2 cut(s) 318, 975
Lsp1109I GCAGC 4 cut(s) 142, 775, 965, 1258
LweI GCATC 1 cut(s) 117
MaeI CTAG 4 cut(s) 453, 642, 815, 932
MaeII ACGT 2 cut(s) 29, 99
MaeIII GTNAC 3 cut(s) 265, 1088, 1261
MalI GATC 6 cut(s) 44, 72, 77, 519, 778, 1191
MboI GATC 6 cut(s) 42, 70, 75, 517, 776, 1189
MboII GAAGA 3 cut(s) 730, 1181, 1184
MflI RGATCY 1 cut(s) 776
MluCI AATT 9 cut(s) 86, 523, 632, 749, 803, 831, 907, 948, 1031
MlyI GAGTC 1 cut(s) 683
MseI TTAA 3 cut(s) 863, 1287, 1350
MslI CAYNNNNRTG 1 cut(s) 225
MspA1I CMGCKG 2 cut(s) 109, 1211
MspI CCGG 1 cut(s) 649
MspR9I CCNGG 2 cut(s) 559, 596
Mva1269I GAATGC 2 cut(s) 844, 981
MvaI CCWGG 2 cut(s) 559, 596
MwoI GCNNNNNNNGC 6 cut(s) 139, 311, 984, 1058, 1171, 1304
NcoI CCATGG 2 cut(s) 145, 327
NdeII GATC 6 cut(s) 42, 70, 75, 517, 776, 1189
NheI GCTAGC 1 cut(s) 931
NlaIII CATG 7 cut(s) 137, 149, 290, 326, 331, 799, 968
NlaIV GGNNCC 2 cut(s) 320, 500
NmuCI GTSAC 1 cut(s) 1261
NspV TTCGAA 1 cut(s) 836
OliI CACNNNNGTG 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 1164
PctI GAATGC 2 cut(s) 844, 981
PfeI GAWTC 4 cut(s) 257, 372, 449, 601
PflFI GACNNNGTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 328
PkrI GCNGC 4 cut(s) 132, 790, 980, 1273
PleI GAGTC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 682
PpuMI RGGWCCY 1 cut(s) 722
Psp1406I AACGTT 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 2 cut(s) 557, 594
PspGI CCWGG 2 cut(s) 557, 594
PspN4I GGNNCC 2 cut(s) 320, 500
PspPI GGNCC 5 cut(s) 80, 353, 722, 733, 1077
PspPPI RGGWCCY 1 cut(s) 722
PstI CTGCAG 1 cut(s) 1276
PsuI RGATCY 1 cut(s) 776
PsyI GACNNNGTC 1 cut(s) 28
PvuII CAGCTG 2 cut(s) 109, 1211
RsaI GTAC 3 cut(s) 236, 653, 891
RsaNI GTAC 3 cut(s) 235, 652, 890
RseI CAYNNNNRTG 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 1164
SaqAI TTAA 3 cut(s) 863, 1287, 1350
SatI GCNGC 4 cut(s) 131, 789, 979, 1272
Sau3AI GATC 6 cut(s) 42, 70, 75, 517, 776, 1189
Sau96I GGNCC 5 cut(s) 80, 353, 722, 733, 1077
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 2 cut(s) 559, 596
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 2 cut(s) 918, 1272
SfuI TTCGAA 1 cut(s) 836
SinI GGWCC 5 cut(s) 80, 353, 722, 733, 1077
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 870
SmoI CTYRAG 1 cut(s) 870
Sse9I AATT 9 cut(s) 86, 523, 632, 749, 803, 831, 907, 948, 1031
SsiI CCGC 2 cut(s) 241, 563
SspMI CTAG 4 cut(s) 453, 642, 815, 932
StyD4I CCNGG 2 cut(s) 557, 594
StyI CCWWGG 2 cut(s) 145, 327
TaaI ACNGT 3 cut(s) 265, 429, 656
TaiI ACGT 2 cut(s) 32, 102
TaqI TCGA 3 cut(s) 45, 520, 836
TaqII GACCGA 1 cut(s) 68
TasI AATT 9 cut(s) 86, 523, 632, 749, 803, 831, 907, 948, 1031
TatI WGTACW 2 cut(s) 234, 889
TfiI GAWTC 4 cut(s) 257, 372, 449, 601
Tru1I TTAA 3 cut(s) 863, 1287, 1350
Tru9I TTAA 3 cut(s) 863, 1287, 1350
TscAI CASTG 4 cut(s) 184, 961, 994, 1006
TseFI GTSAC 1 cut(s) 1261
TseI GCWGC 4 cut(s) 130, 788, 978, 1271
Tsp45I GTSAC 1 cut(s) 1261
TspDTI ATGAA 4 cut(s) 506, 797, 888, 1044
TspGWI ACGGA 1 cut(s) 1090
TspRI CASTG 4 cut(s) 184, 961, 994, 1006
Tth111I GACNNNGTC 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 328
VpaK11BI GGWCC 5 cut(s) 80, 353, 722, 733, 1077
XagI CCTNNNNNAGG 1 cut(s) 740
XapI RAATTY 3 cut(s) 86, 803, 948
XbaI TCTAGA 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 658
XspI CTAG 4 cut(s) 453, 642, 815, 932
ZraI GACGTC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.