MD14G1176300.v1.1

FAS-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012615
Physical Location & Seq
Forward (+)
26941258 .. 26943403
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1176300.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGGCAATGAGGGAAGGCATAAGATCAGCTGCAGAGGCATCTTGCAATGGGATCGTTCGCCGGATGGTAAGCCTTCCGAGCAGCCTTATGGGAGGATTTTCGAGAGCAATGGGGCACGGCAGACACCTTATAGGAATAGGATCAGGAAGAAGAACTCAAACCCTACCATCAAACTTTCCAATGCATTATCCAGAAGAGCCTCTAATCCTTCCAGAAGTATCGGTCTCTTTTCTAACTAGCTTCGAGCAGCAGTATGGCACCATGCATCCATTCTTTTACGCATGCCGGTTTATGGAAACCCTAAAGATTGCTGAGGATGAGCACAAGTTCTTGTTCTTGTATCTCCACTCACCGGAACACCCTTTCACACCTTCTTTCTGTAGGGAGACGCTGTCTTCTGAGGCGGTGGTGCAGTTTCTTGATGCTAACTTTGTTTCCTGGGGAGCAATTGCAGATAGAGATGAAGGTCTACAAATGAGTGCAACTCTTCGCCCGGCTTGCTTTCCATTCTGTGCTGTTATAGCTCCTGTTGCTGGTGATAACATAGCAGTGATGCAGCAGATGGAAGGGCCAATTTCCCCAACCGAGCTGGTGGGGATTCTGCAGCGGACGCTGGAGGAACAAGGGCTGGCGTTTCGCAGTTTGAAGGCCAAGAAGGCAGAAAAAATAATAGCAGACCGCCGTCTGAGGGAAGAACAAGATGCAGCATATCTTGCAGCTATACAAATAGACAAGCAAAAGGAGAGACTAAAGAATGTACCTCCACCTGTTCGACCTCAAAAACCAGCAGAAGCCCCAAACAAAGCAAATCATGAGAGGCTTCAAAACCATTCTGCCCAAAAACAGCATGGAAAAACTAGAGAGGCTTCTATTGATCCTCAAGATCCTCAGGCTGCCCGGATACTGATCAGATTTCCGAATGGCGAAAGACGAGAGCAATGCTTCTTGTGCACAGACAAACTGCAAGCGGTTTACAGATACATAGATTCATTGGGTGTTCCTGGTATAGGAGGAAACTACAGATTGGTGTCAAGCTTCCCAAGAAAAGTGTATGGAGTTGATCAAATGGGGATCACACTCAAGGATGCTGGCCTGCATCCTAGAGCAAGCCTATTTCTTGAATTGGCCCCAACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UBX

Protein Analysis

380

Amino Acids

42.33

Weight (kDa)

8.62

Isoelectric Point (pI)

56.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF1 PF21021 77 - 148 1.3e-11 Fas-associated factor 1
UBX PF00789 296 - 374 2.6e-18 UBX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012615)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05720 FvH4_5g05720 FvH4_5g05720
malus_domestica MD06G1170500.v1.1 MD14G1176300.v1.1
prunus_persica Prupe.5G170100_v2.0.a1
pyrus_communis pycom06g15190 pycom14g14720
rosa_chinensis RchiOBHm_Chr7g0193081
rosa_laevigata RLG00000004296
rosa_multiflora Rmu_ssc0000002.1_g000009
rosa_roxburghii Rroxscaffold_3G00262380
rosa_rugosa Rorug07G0007500
rosa_samantha Rh7AG132700 Rh7BG133600 Rh7CG136700 Rh7DG134600
rosa_wichuraiana Rw0G015840 Rw7G011340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 257
AccI GTMKAC 1 cut(s) 469
AciI CCGC 4 cut(s) 404, 607, 679, 968
AclWI GGATC 5 cut(s) 59, 148, 869, 878, 1079
AfaI GTAC 1 cut(s) 759
AfiI CCNNNNNNNGG 5 cut(s) 292, 352, 533, 688, 1007
AgsI TTSAA 3 cut(s) 646, 824, 1121
AjnI CCWGG 2 cut(s) 437, 1000
AluBI AGCT 6 cut(s) 29, 240, 524, 589, 719, 1035
AluI AGCT 6 cut(s) 29, 240, 524, 589, 719, 1035
Alw21I GWGCWC 2 cut(s) 324, 953
Alw26I GTCTC 3 cut(s) 229, 380, 739
Alw44I GTGCAC 1 cut(s) 949
AlwI GGATC 5 cut(s) 59, 148, 869, 878, 1079
AoxI GGCC 4 cut(s) 569, 648, 1090, 1125
ApaLI GTGCAC 1 cut(s) 949
ApeKI GCWGC 8 cut(s) 29, 81, 247, 556, 604, 704, 716, 893
AspS9I GGNCC 2 cut(s) 569, 1126
AsuC2I CCSGG 2 cut(s) 494, 898
AsuHPI GGTGA 2 cut(s) 342, 548
AxyI CCTNAGG 1 cut(s) 888
BaeGI GKGCMC 2 cut(s) 117, 953
BanI GGYRCC 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 2 cut(s) 324, 953
BbvCI CCTCAGC 1 cut(s) 312
BbvI GCAGC 8 cut(s) 16, 93, 259, 568, 616, 716, 728, 880
BccI CCATC 3 cut(s) 58, 175, 556
BceAI ACGGC 2 cut(s) 133, 666
BcgI CGANNNNNNTGC 4 cut(s) 34, 68, 921, 955
BciT130I CCWGG 2 cut(s) 439, 1002
BciVI GTATCC 1 cut(s) 894
BclI TGATCA 2 cut(s) 906, 1060
BcnI CCSGG 2 cut(s) 494, 898
BcoDI GTCTC 3 cut(s) 229, 380, 739
BfaI CTAG 3 cut(s) 237, 858, 1101
BfmI CTRYAG 4 cut(s) 30, 379, 602, 1018
BfuI GTATCC 1 cut(s) 894
BglI GCCNNNNNGGC 1 cut(s) 656
BisI GCNGC 8 cut(s) 30, 82, 248, 557, 605, 705, 717, 894
BlsI GCNGC 8 cut(s) 31, 83, 249, 558, 606, 706, 718, 895
Bme1390I CCNGG 4 cut(s) 439, 494, 898, 1002
BmgT120I GGNCC 2 cut(s) 569, 1126
BmiI GGNNCC 2 cut(s) 259, 1128
BmrFI CCNGG 4 cut(s) 439, 494, 898, 1002
BmsI GCATC 7 cut(s) 47, 274, 412, 543, 691, 1075, 1105
BoxI GACNNNNGTC 1 cut(s) 681
BpiI GAAGAC 1 cut(s) 387
BplI GAGNNNNNCTC 2 cut(s) 469, 501
BpmI CTGGAG 1 cut(s) 635
Bpu10I CCTNAGC 1 cut(s) 312
BpuEI CTTGAG 2 cut(s) 864, 1064
BpuMI CCSGG 2 cut(s) 494, 898
BsaBI GATNNNNATC 1 cut(s) 905
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 1 cut(s) 438
BsaWI WCCGGW 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 5 cut(s) 292, 352, 533, 688, 1007
Bse118I RCCGGY 1 cut(s) 285
Bse21I CCTNAGG 1 cut(s) 888
Bse3DI GCAATG 4 cut(s) 12, 52, 114, 944
Bse8I GATNNNNATC 1 cut(s) 905
BseBI CCWGG 2 cut(s) 439, 1002
BseDI CCNNGG 1 cut(s) 438
BseGI GGATG 5 cut(s) 69, 265, 322, 1090, 1096
BseJI GATNNNNATC 1 cut(s) 905
BseLI CCNNNNNNNGG 5 cut(s) 292, 352, 533, 688, 1007
BseMI GCAATG 4 cut(s) 12, 52, 114, 944
BseMII CTCAG 4 cut(s) 303, 390, 677, 902
BseSI GKGCMC 2 cut(s) 117, 953
BseXI GCAGC 8 cut(s) 16, 93, 259, 568, 616, 716, 728, 880
BsgI GTGCAG 1 cut(s) 431
BshFI GGCC 4 cut(s) 571, 650, 1092, 1127
BshNI GGYRCC 1 cut(s) 257
BsiHKAI GWGCWC 2 cut(s) 324, 953
BsiSI CCGG 5 cut(s) 61, 286, 353, 494, 898
BslI CCNNNNNNNGG 5 cut(s) 292, 352, 533, 688, 1007
BsmAI GTCTC 3 cut(s) 229, 380, 739
BsmBI CGTCTC 1 cut(s) 380
BsnI GGCC 4 cut(s) 571, 650, 1092, 1127
Bso31I GGTCTC 1 cut(s) 229
Bsp1286I GDGCHC 3 cut(s) 117, 324, 953
Bsp143I GATC 8 cut(s) 23, 51, 140, 874, 883, 906, 1060, 1071
BspACI CCGC 4 cut(s) 404, 607, 679, 968
BspANI GGCC 4 cut(s) 571, 650, 1092, 1127
BspCNI CTCAG 4 cut(s) 304, 391, 678, 901
BspHI TCATGA 1 cut(s) 811
BspLI GGNNCC 2 cut(s) 259, 1128
BspMAI CTGCAG 2 cut(s) 34, 606
BspPI GGATC 5 cut(s) 59, 148, 869, 878, 1079
BspQI GCTCTTC 1 cut(s) 189
BspT107I GGYRCC 1 cut(s) 257
BspTNI GGTCTC 1 cut(s) 229
BsrDI GCAATG 4 cut(s) 12, 52, 114, 944
BsrFI RCCGGY 1 cut(s) 285
BssAI RCCGGY 1 cut(s) 285
BssECI CCNNGG 1 cut(s) 438
BssMI GATC 8 cut(s) 23, 51, 140, 874, 883, 906, 1060, 1071
Bst2UI CCWGG 2 cut(s) 439, 1002
Bst4CI ACNGT 1 cut(s) 1135
Bst6I CTCTTC 2 cut(s) 189, 492
BstAPI GCANNNNNTGC 1 cut(s) 713
BstC8I GCNNGC 7 cut(s) 283, 499, 630, 966, 1090, 1094, 1108
BstDEI CTNAG 4 cut(s) 312, 399, 686, 888
BstENI CCTNNNNNAGG 1 cut(s) 1005
BstF5I GGATG 5 cut(s) 69, 265, 322, 1090, 1096
BstKTI GATC 8 cut(s) 26, 54, 143, 877, 886, 909, 1063, 1074
BstMAI GTCTC 3 cut(s) 229, 380, 739
BstMBI GATC 8 cut(s) 23, 51, 140, 874, 883, 906, 1060, 1071
BstMWI GCNNNNNNNGC 9 cut(s) 35, 78, 498, 521, 530, 610, 656, 713, 948
BstNI CCWGG 2 cut(s) 439, 1002
BstNSI RCATGY 1 cut(s) 285
BstPAI GACNNNNGTC 1 cut(s) 681
BstSCI CCNGG 4 cut(s) 437, 492, 896, 1000
BstSFI CTRYAG 4 cut(s) 30, 379, 602, 1018
BstSLI GKGCMC 2 cut(s) 117, 953
BstV1I GCAGC 8 cut(s) 16, 93, 259, 568, 616, 716, 728, 880
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 883
BstYI RGATCY 1 cut(s) 883
Bsu36I CCTNAGG 1 cut(s) 888
BsuI GTATCC 1 cut(s) 894
BsuRI GGCC 4 cut(s) 571, 650, 1092, 1127
BtsCI GGATG 5 cut(s) 69, 265, 322, 1090, 1096
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 1 cut(s) 555
Cac8I GCNNGC 7 cut(s) 283, 499, 630, 966, 1090, 1094, 1108
CciI TCATGA 1 cut(s) 811
Cfr10I RCCGGY 1 cut(s) 285
Cfr13I GGNCC 2 cut(s) 569, 1126
CseI GACGC 2 cut(s) 397, 619
Csp6I GTAC 1 cut(s) 758
CviAII CATG 4 cut(s) 262, 282, 812, 848
CviQI GTAC 1 cut(s) 758
DdeI CTNAG 4 cut(s) 312, 399, 686, 888
DpnI GATC 8 cut(s) 25, 53, 142, 876, 885, 908, 1062, 1073
DpnII GATC 8 cut(s) 23, 51, 140, 874, 883, 906, 1060, 1071
Eam1104I CTCTTC 2 cut(s) 189, 492
EarI CTCTTC 2 cut(s) 189, 492
Eco31I GGTCTC 1 cut(s) 229
Eco81I CCTNAGG 1 cut(s) 888
EcoNI CCTNNNNNAGG 1 cut(s) 1005
EcoRII CCWGG 2 cut(s) 437, 1000
EcoT22I ATGCAT 2 cut(s) 186, 267
Esp3I CGTCTC 1 cut(s) 380
FaeI CATG 4 cut(s) 265, 285, 815, 851
FatI CATG 4 cut(s) 261, 281, 811, 847
FbaI TGATCA 2 cut(s) 906, 1060
FblI GTMKAC 1 cut(s) 469
Fnu4HI GCNGC 8 cut(s) 30, 82, 248, 557, 605, 705, 717, 894
FokI GGATG 5 cut(s) 76, 252, 329, 1083, 1097
Fsp4HI GCNGC 8 cut(s) 30, 82, 248, 557, 605, 705, 717, 894
FspBI CTAG 3 cut(s) 237, 858, 1101
GluI GCNGC 8 cut(s) 30, 82, 248, 557, 605, 705, 717, 894
GsuI CTGGAG 1 cut(s) 635
HaeIII GGCC 4 cut(s) 571, 650, 1092, 1127
HapII CCGG 5 cut(s) 61, 286, 353, 494, 898
HgaI GACGC 2 cut(s) 397, 619
Hin1II CATG 4 cut(s) 265, 285, 815, 851
HindIII AAGCTT 1 cut(s) 1033
HinfI GANTC 2 cut(s) 598, 986
HpaII CCGG 5 cut(s) 61, 286, 353, 494, 898
HphI GGTGA 2 cut(s) 342, 548
Hpy166II GTNNAC 3 cut(s) 470, 951, 973
Hpy188I TCNGA 5 cut(s) 78, 400, 687, 911, 918
Hpy188III TCNNGA 8 cut(s) 102, 144, 191, 212, 419, 812, 881, 1118
Hpy8I GTNNAC 3 cut(s) 470, 951, 973
HpyAV CCTTC 8 cut(s) 8, 83, 218, 381, 458, 560, 640, 649
HpyCH4III ACNGT 1 cut(s) 1135
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 78, 498, 521, 530, 610, 656, 713, 948
HpyF3I CTNAG 4 cut(s) 312, 399, 686, 888
Hsp92II CATG 4 cut(s) 265, 285, 815, 851
Ksp22I TGATCA 2 cut(s) 906, 1060
Kzo9I GATC 8 cut(s) 23, 51, 140, 874, 883, 906, 1060, 1071
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 2 cut(s) 443, 529
Lsp1109I GCAGC 8 cut(s) 16, 93, 259, 568, 616, 716, 728, 880
LweI GCATC 7 cut(s) 47, 274, 412, 543, 691, 1075, 1105
MaeI CTAG 3 cut(s) 237, 858, 1101
MalI GATC 8 cut(s) 25, 53, 142, 876, 885, 908, 1062, 1073
MboI GATC 8 cut(s) 23, 51, 140, 874, 883, 906, 1060, 1071
MboII GAAGA 6 cut(s) 159, 162, 206, 387, 479, 704
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 1 cut(s) 883
MhlI GDGCHC 3 cut(s) 117, 324, 953
MluCI AATT 3 cut(s) 447, 573, 1121
Mph1103I ATGCAT 2 cut(s) 186, 267
MseI TTAA 1 cut(s) 1138
MslI CAYNNNNRTG 1 cut(s) 548
MspA1I CMGCKG 2 cut(s) 29, 607
MspI CCGG 5 cut(s) 61, 286, 353, 494, 898
MspR9I CCNGG 4 cut(s) 439, 494, 898, 1002
MunI CAATTG 1 cut(s) 447
MvaI CCWGG 2 cut(s) 439, 1002
MwoI GCNNNNNNNGC 9 cut(s) 35, 78, 498, 521, 530, 610, 656, 713, 948
NciI CCSGG 2 cut(s) 494, 898
NdeII GATC 8 cut(s) 23, 51, 140, 874, 883, 906, 1060, 1071
NlaIII CATG 4 cut(s) 265, 285, 815, 851
NlaIV GGNNCC 2 cut(s) 259, 1128
NsiI ATGCAT 2 cut(s) 186, 267
NspI RCATGY 1 cut(s) 285
PaeI GCATGC 1 cut(s) 285
PagI TCATGA 1 cut(s) 811
PciSI GCTCTTC 1 cut(s) 189
PfeI GAWTC 2 cut(s) 598, 986
PflFI GACNNNGTC 1 cut(s) 391
PkrI GCNGC 8 cut(s) 31, 83, 249, 558, 606, 706, 718, 895
PshAI GACNNNNGTC 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 437, 1000
PspGI CCWGG 2 cut(s) 437, 1000
PspN4I GGNNCC 2 cut(s) 259, 1128
PspPI GGNCC 2 cut(s) 569, 1126
PstI CTGCAG 2 cut(s) 34, 606
PsuI RGATCY 1 cut(s) 883
PsyI GACNNNGTC 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 1 cut(s) 759
RsaNI GTAC 1 cut(s) 758
RseI CAYNNNNRTG 1 cut(s) 548
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 1138
SatI GCNGC 8 cut(s) 30, 82, 248, 557, 605, 705, 717, 894
Sau3AI GATC 8 cut(s) 23, 51, 140, 874, 883, 906, 1060, 1071
Sau96I GGNCC 2 cut(s) 569, 1126
ScrFI CCNGG 4 cut(s) 439, 494, 898, 1002
SduI GDGCHC 3 cut(s) 117, 324, 953
SfaNI GCATC 7 cut(s) 47, 274, 412, 543, 691, 1075, 1105
SfcI CTRYAG 4 cut(s) 30, 379, 602, 1018
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 2 cut(s) 879, 1079
SmoI CTYRAG 2 cut(s) 879, 1079
SphI GCATGC 1 cut(s) 285
Sse9I AATT 3 cut(s) 447, 573, 1121
SsiI CCGC 4 cut(s) 404, 607, 679, 968
SspMI CTAG 3 cut(s) 237, 858, 1101
StyD4I CCNGG 4 cut(s) 437, 492, 896, 1000
TaaI ACNGT 1 cut(s) 1135
TaqI TCGA 3 cut(s) 101, 243, 772
TaqII GACCGA 1 cut(s) 211
TasI AATT 3 cut(s) 447, 573, 1121
TfiI GAWTC 2 cut(s) 598, 986
Tru1I TTAA 1 cut(s) 1138
Tru9I TTAA 1 cut(s) 1138
TscAI CASTG 1 cut(s) 555
TseI GCWGC 8 cut(s) 29, 81, 247, 556, 604, 704, 716, 893
TspDTI ATGAA 2 cut(s) 477, 978
TspRI CASTG 1 cut(s) 555
Tth111I GACNNNGTC 1 cut(s) 391
VneI GTGCAC 1 cut(s) 949
XagI CCTNNNNNAGG 1 cut(s) 1005
XceI RCATGY 1 cut(s) 285
XcmI CCANNNNNNNNNTGG 1 cut(s) 845
XmiI GTMKAC 1 cut(s) 469
XspI CTAG 3 cut(s) 237, 858, 1101
Zsp2I ATGCAT 2 cut(s) 186, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.