pycom06g15190

FAS-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012615
Physical Location & Seq
Forward (+)
19787832 .. 19789500
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g15190.1

Sequence Viewer

Length: 1125 bp
ATGAGGGAAGGCATAAGATCGGCAGCGGAGCCCTCTTGCAGTGGGATAGTTCGCCGGATGGTAAGCCTTCCGAGAAGCCTTATGGGAGGATTTTCGAGAGCAGTGGGGCACGGTAGACACCTCATAGGAATAGGATCAGGAAGAAGAACTCAAACCCTGCCATCAAACTTTCCAATGCAGAATCCAGAAGAGCCTCTGATCCTTCCAGAAGTATCGGCTTCTTTTCTAACTAGCTTCGAGCAGCAGTATGGCACCATGCATCCGTTCTTTTATGCGTGTAGTTTTATGGAGGCCTTAAAGATGGCAGAAGATGAGCATAAGTTCTTGTTCATTTATCTCCACTCACCGGAACACCCTTTCACACCGTCTTTCTGTAGGGAGACGCTGTCTTCGGAGGTGGTGGTGCAGTTTCTTGATGCTAACTTTGTTTCCTGGGGAGCAATTGCAGATGGAGAGGAAGGTCTTCAAATGGCTGCAACTCTGCGTCCTACTTGCTTTCCATTCTGTGCTATTATAGCTCCCGTTGCTGGTGATAACATAGCTGTGTTGCAGCAGATGGAAGGGCCAATTACCCCAACTGAGCTGGTGGGGATTCTGCAGCGGACGGTGGAGGAACAAGGGCTGGCGTTTCGCAGTTCAAAGGCCAAGAAGGCAGAAAAAATAATGGCAGACCGCCGTCTAAGGGAAGAACAAGATGCTGCATATCTTGCAGCTCTACAAATAGACGAGGAAAAGGAGAGACTAAAGAATTTACCTCCAGCTGTTAGACCTCATAAACCAGCAGAAGCCCCAAACAAAGCAAGTCATGAGAGGCTTCAGAACCATTCTGCCAAACAACAGCATGGAAAAACTAGAGAGGCTGCCATTGATCCTCAAGATCCTCAGGCTGCCCGGATACTGATAAGGTTTCCGAACGGCGAAAGACGAGAGCAATGCTTCTTGTGCACAGACAAACTGCAGGCAGTTTACAGATACATTGATTCATTGGGTGTTCCTGATATAGGAGGAAACTACAGATTGATATCAAGCTTCCCAAGAAAAGTGTACGGAGTTGATCAAATGGGGATCACACTCAAGGATGCTGGCCTGCATCCAAGAGCAAGCCTATTTCTTGAACTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UBX

Protein Analysis

375

Amino Acids

41.7

Weight (kDa)

6.84

Isoelectric Point (pI)

56.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF1 PF21021 75 - 146 4.4e-13 Fas-associated factor 1
UBX PF00789 294 - 372 1e-18 UBX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012615)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05720 FvH4_5g05720 FvH4_5g05720
malus_domestica MD06G1170500.v1.1 MD14G1176300.v1.1
prunus_persica Prupe.5G170100_v2.0.a1
pyrus_communis pycom06g15190 pycom14g14720
rosa_chinensis RchiOBHm_Chr7g0193081
rosa_laevigata RLG00000004296
rosa_multiflora Rmu_ssc0000002.1_g000009
rosa_roxburghii Rroxscaffold_3G00262380
rosa_rugosa Rorug07G0007500
rosa_samantha Rh7AG132700 Rh7BG133600 Rh7CG136700 Rh7DG134600
rosa_wichuraiana Rw0G015840 Rw7G011340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 115
AciI CCGC 3 cut(s) 26, 601, 673
AclWI GGATC 5 cut(s) 142, 193, 863, 872, 1073
AcsI RAATTY 1 cut(s) 748
AcuI CTGAAG 1 cut(s) 800
AfaI GTAC 1 cut(s) 1046
AfiI CCNNNNNNNGG 4 cut(s) 346, 527, 682, 1001
AgsI TTSAA 3 cut(s) 467, 639, 1115
AjnI CCWGG 1 cut(s) 431
AluBI AGCT 7 cut(s) 234, 518, 542, 583, 713, 761, 1029
AluI AGCT 7 cut(s) 234, 518, 542, 583, 713, 761, 1029
Alw21I GWGCWC 1 cut(s) 947
Alw26I GTCTC 2 cut(s) 374, 733
Alw44I GTGCAC 1 cut(s) 943
AlwI GGATC 5 cut(s) 142, 193, 863, 872, 1073
AoxI GGCC 4 cut(s) 291, 563, 642, 1084
ApaLI GTGCAC 1 cut(s) 943
ApeKI GCWGC 9 cut(s) 23, 241, 473, 550, 598, 698, 710, 860, 887
ApoI RAATTY 1 cut(s) 748
ArsI GACNNNNNNTTYG 2 cut(s) 373, 405
Asp700I GAANNNNTTC 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 563
AsuC2I CCSGG 1 cut(s) 892
AsuHPI GGTGA 2 cut(s) 336, 542
AxyI CCTNAGG 1 cut(s) 882
BaeGI GKGCMC 2 cut(s) 111, 947
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 381, 455
Bbv12I GWGCWC 1 cut(s) 947
BbvI GCAGC 9 cut(s) 35, 253, 460, 562, 610, 685, 722, 847, 874
BccI CCATC 5 cut(s) 52, 169, 295, 443, 550
BceAI ACGGC 2 cut(s) 660, 931
BcgI CGANNNNNNTGC 2 cut(s) 915, 949
BciT130I CCWGG 1 cut(s) 433
BciVI GTATCC 1 cut(s) 888
BclI TGATCA 1 cut(s) 1054
BcnI CCSGG 1 cut(s) 892
BcoDI GTCTC 2 cut(s) 374, 733
BfaI CTAG 2 cut(s) 231, 852
BfmI CTRYAG 4 cut(s) 373, 596, 956, 1012
BfuI GTATCC 1 cut(s) 888
BglI GCCNNNNNGGC 1 cut(s) 650
BisI GCNGC 9 cut(s) 24, 242, 474, 551, 599, 699, 711, 861, 888
BlsI GCNGC 9 cut(s) 25, 243, 475, 552, 600, 700, 712, 862, 889
Bme1390I CCNGG 2 cut(s) 433, 892
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 2 cut(s) 30, 253
BmrFI CCNGG 2 cut(s) 433, 892
BmsI GCATC 5 cut(s) 268, 406, 685, 1069, 1099
BoxI GACNNNNGTC 1 cut(s) 675
BpiI GAAGAC 2 cut(s) 381, 455
BpmI CTGGAG 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 858, 1058
BpuMI CCSGG 1 cut(s) 892
BsaBI GATNNNNATC 1 cut(s) 1021
BsaJI CCNNGG 1 cut(s) 432
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 4 cut(s) 346, 527, 682, 1001
Bse21I CCTNAGG 1 cut(s) 882
Bse3DI GCAATG 1 cut(s) 938
Bse8I GATNNNNATC 1 cut(s) 1021
BseBI CCWGG 1 cut(s) 433
BseDI CCNNGG 1 cut(s) 432
BseGI GGATG 4 cut(s) 63, 259, 1084, 1090
BseJI GATNNNNATC 1 cut(s) 1021
BseLI CCNNNNNNNGG 4 cut(s) 346, 527, 682, 1001
BseMI GCAATG 1 cut(s) 938
BseMII CTCAG 2 cut(s) 570, 896
BseSI GKGCMC 2 cut(s) 111, 947
BseXI GCAGC 9 cut(s) 35, 253, 460, 562, 610, 685, 722, 847, 874
BsgI GTGCAG 1 cut(s) 425
BshFI GGCC 4 cut(s) 293, 565, 644, 1086
BshNI GGYRCC 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 947
BsiSI CCGG 3 cut(s) 55, 347, 892
BslI CCNNNNNNNGG 4 cut(s) 346, 527, 682, 1001
BsmAI GTCTC 2 cut(s) 374, 733
BsmBI CGTCTC 1 cut(s) 374
BsnI GGCC 4 cut(s) 293, 565, 644, 1086
Bsp1286I GDGCHC 3 cut(s) 33, 111, 947
Bsp143I GATC 7 cut(s) 17, 134, 198, 868, 877, 1054, 1065
BspACI CCGC 3 cut(s) 26, 601, 673
BspANI GGCC 4 cut(s) 293, 565, 644, 1086
BspCNI CTCAG 2 cut(s) 571, 895
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 2 cut(s) 30, 253
BspMAI CTGCAG 2 cut(s) 600, 960
BspPI GGATC 5 cut(s) 142, 193, 863, 872, 1073
BspQI GCTCTTC 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 938
BssECI CCNNGG 1 cut(s) 432
BssMI GATC 7 cut(s) 17, 134, 198, 868, 877, 1054, 1065
Bst2UI CCWGG 1 cut(s) 433
Bst4CI ACNGT 3 cut(s) 113, 366, 607
Bst6I CTCTTC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 707
BstC8I GCNNGC 5 cut(s) 624, 960, 1084, 1088, 1102
BstDEI CTNAG 3 cut(s) 579, 680, 882
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 4 cut(s) 63, 259, 1084, 1090
BstKTI GATC 7 cut(s) 20, 137, 201, 871, 880, 1057, 1068
BstMAI GTCTC 2 cut(s) 374, 733
BstMBI GATC 7 cut(s) 17, 134, 198, 868, 877, 1054, 1065
BstMWI GCNNNNNNNGC 5 cut(s) 515, 524, 650, 707, 942
BstNI CCWGG 1 cut(s) 433
BstPAI GACNNNNGTC 1 cut(s) 675
BstSCI CCNGG 2 cut(s) 431, 890
BstSFI CTRYAG 4 cut(s) 373, 596, 956, 1012
BstSLI GKGCMC 2 cut(s) 111, 947
BstV1I GCAGC 9 cut(s) 35, 253, 460, 562, 610, 685, 722, 847, 874
BstV2I GAAGAC 2 cut(s) 381, 455
BstX2I RGATCY 1 cut(s) 877
BstYI RGATCY 1 cut(s) 877
Bsu36I CCTNAGG 1 cut(s) 882
BsuI GTATCC 1 cut(s) 888
BsuRI GGCC 4 cut(s) 293, 565, 644, 1086
BtsCI GGATG 4 cut(s) 63, 259, 1084, 1090
BtsI GCAGTG 2 cut(s) 46, 108
BtsIMutI CAGTG 2 cut(s) 46, 108
Cac8I GCNNGC 5 cut(s) 624, 960, 1084, 1088, 1102
CciI TCATGA 1 cut(s) 805
Cfr13I GGNCC 1 cut(s) 563
CseI GACGC 2 cut(s) 391, 473
Csp6I GTAC 1 cut(s) 1045
CviAII CATG 3 cut(s) 256, 806, 842
CviQI GTAC 1 cut(s) 1045
DdeI CTNAG 3 cut(s) 579, 680, 882
DpnI GATC 7 cut(s) 19, 136, 200, 870, 879, 1056, 1067
DpnII GATC 7 cut(s) 17, 134, 198, 868, 877, 1054, 1065
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco147I AGGCCT 1 cut(s) 293
Eco24I GRGCYC 1 cut(s) 33
Eco32I GATATC 1 cut(s) 1023
Eco57I CTGAAG 1 cut(s) 800
Eco81I CCTNAGG 1 cut(s) 882
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRII CCWGG 1 cut(s) 431
EcoRV GATATC 1 cut(s) 1023
EcoT22I ATGCAT 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 33
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 3 cut(s) 259, 809, 845
FatI CATG 3 cut(s) 255, 805, 841
FbaI TGATCA 1 cut(s) 1054
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 9 cut(s) 24, 242, 474, 551, 599, 699, 711, 861, 888
FokI GGATG 4 cut(s) 70, 246, 1077, 1091
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 9 cut(s) 24, 242, 474, 551, 599, 699, 711, 861, 888
FspBI CTAG 2 cut(s) 231, 852
GluI GCNGC 9 cut(s) 24, 242, 474, 551, 599, 699, 711, 861, 888
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 4 cut(s) 293, 565, 644, 1086
HapII CCGG 3 cut(s) 55, 347, 892
HgaI GACGC 2 cut(s) 391, 473
Hin1II CATG 3 cut(s) 259, 809, 845
HindIII AAGCTT 1 cut(s) 1027
HinfI GANTC 3 cut(s) 181, 592, 980
HpaII CCGG 3 cut(s) 55, 347, 892
HphI GGTGA 2 cut(s) 336, 542
Hpy166II GTNNAC 4 cut(s) 116, 945, 967, 1045
Hpy188I TCNGA 5 cut(s) 72, 198, 394, 819, 912
Hpy188III TCNNGA 9 cut(s) 96, 138, 185, 206, 413, 806, 875, 995, 1112
Hpy8I GTNNAC 4 cut(s) 116, 945, 967, 1045
HpyAV CCTTC 5 cut(s) 77, 212, 452, 554, 643
HpyCH4III ACNGT 3 cut(s) 113, 366, 607
HpyF10VI GCNNNNNNNGC 5 cut(s) 515, 524, 650, 707, 942
HpyF3I CTNAG 3 cut(s) 579, 680, 882
Hsp92II CATG 3 cut(s) 259, 809, 845
Ksp22I TGATCA 1 cut(s) 1054
Kzo9I GATC 7 cut(s) 17, 134, 198, 868, 877, 1054, 1065
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 3 cut(s) 28, 437, 523
Lsp1109I GCAGC 9 cut(s) 35, 253, 460, 562, 610, 685, 722, 847, 874
LweI GCATC 5 cut(s) 268, 406, 685, 1069, 1099
MaeI CTAG 2 cut(s) 231, 852
MalI GATC 7 cut(s) 19, 136, 200, 870, 879, 1056, 1067
MboI GATC 7 cut(s) 17, 134, 198, 868, 877, 1054, 1065
MboII GAAGA 7 cut(s) 153, 156, 200, 320, 381, 455, 698
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 3 cut(s) 33, 111, 947
MluCI AATT 3 cut(s) 441, 567, 748
Mph1103I ATGCAT 1 cut(s) 261
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 1 cut(s) 296
MslI CAYNNNNRTG 1 cut(s) 542
MspA1I CMGCKG 3 cut(s) 26, 601, 761
MspI CCGG 3 cut(s) 55, 347, 892
MspR9I CCNGG 2 cut(s) 433, 892
MunI CAATTG 1 cut(s) 441
MvaI CCWGG 1 cut(s) 433
MwoI GCNNNNNNNGC 5 cut(s) 515, 524, 650, 707, 942
NciI CCSGG 1 cut(s) 892
NdeII GATC 7 cut(s) 17, 134, 198, 868, 877, 1054, 1065
NlaIII CATG 3 cut(s) 259, 809, 845
NlaIV GGNNCC 2 cut(s) 30, 253
NsiI ATGCAT 1 cut(s) 261
PagI TCATGA 1 cut(s) 805
PceI AGGCCT 1 cut(s) 293
PciSI GCTCTTC 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 3 cut(s) 181, 592, 980
PflFI GACNNNGTC 1 cut(s) 385
PkrI GCNGC 9 cut(s) 25, 243, 475, 552, 600, 700, 712, 862, 889
PshAI GACNNNNGTC 1 cut(s) 675
Psp6I CCWGG 1 cut(s) 431
PspGI CCWGG 1 cut(s) 431
PspN4I GGNNCC 2 cut(s) 30, 253
PspPI GGNCC 1 cut(s) 563
PstI CTGCAG 2 cut(s) 600, 960
PsuI RGATCY 1 cut(s) 877
PsyI GACNNNGTC 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 1 cut(s) 1046
RsaNI GTAC 1 cut(s) 1045
RseI CAYNNNNRTG 1 cut(s) 542
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 1 cut(s) 296
SatI GCNGC 9 cut(s) 24, 242, 474, 551, 599, 699, 711, 861, 888
Sau3AI GATC 7 cut(s) 17, 134, 198, 868, 877, 1054, 1065
Sau96I GGNCC 1 cut(s) 563
ScrFI CCNGG 2 cut(s) 433, 892
SduI GDGCHC 3 cut(s) 33, 111, 947
SfaNI GCATC 5 cut(s) 268, 406, 685, 1069, 1099
SfcI CTRYAG 4 cut(s) 373, 596, 956, 1012
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 2 cut(s) 873, 1073
SmoI CTYRAG 2 cut(s) 873, 1073
Sse9I AATT 3 cut(s) 441, 567, 748
SseBI AGGCCT 1 cut(s) 293
SsiI CCGC 3 cut(s) 26, 601, 673
SspMI CTAG 2 cut(s) 231, 852
StuI AGGCCT 1 cut(s) 293
StyD4I CCNGG 2 cut(s) 431, 890
TaaI ACNGT 3 cut(s) 113, 366, 607
TaqI TCGA 2 cut(s) 95, 237
TasI AATT 3 cut(s) 441, 567, 748
TfiI GAWTC 3 cut(s) 181, 592, 980
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TscAI CASTG 2 cut(s) 46, 108
TseI GCWGC 9 cut(s) 23, 241, 473, 550, 598, 698, 710, 860, 887
TspDTI ATGAA 2 cut(s) 319, 972
TspGWI ACGGA 2 cut(s) 252, 1062
TspRI CASTG 2 cut(s) 46, 108
Tth111I GACNNNGTC 1 cut(s) 385
VneI GTGCAC 1 cut(s) 943
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 1 cut(s) 748
XmiI GTMKAC 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 2 cut(s) 231, 852
Zsp2I ATGCAT 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.