Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0008770

Physical Location & Seq

Genomic Coordinates Forward (+)

28279077 .. 28286139

Gene Structure 7063 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1191800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3828 bp

ATGGGCGGCGACGACGATTCTCCCCAATGCCTCCTCCTCCCTTCGCAAAACGATGCCCAGCCGGAGTCCTCCGCATCCGCGACACGTGTCCCGCTCGCTCAGACGCCGATCCTCCTGCTCGTCTGCTTCCACAAGGCGCTGCAGGCCGAGCTCCTCGATCTCCGCCTCGTGACCACCGCCGCCTTGGAGAGTGGCTCACGTGACCTCCTAGACCGCGACTTCGTTCTCCTGCTTCTGCGGCGGTTTGAGTTCCTCAAGCTCGCGTATGAGTCCCACTGCTCTGCAGAGGACGAGGTAATTTTTTTAGCGTTAGATGGGCGAACAAAAAATGTAGCTAGTACATATTCACTAGAACATATGAGTATAGACGGTCTCTTCGACTCAATTTTCAATCGCTTGGATGTTTTATTAGAGGAAAACGAGAATGAGAAATTTTCCAAGCAATTTCAAGAACTTGTGTTTGGCATTGGGACGCTGAAGGAATTCGTTTCCCAGCATATGCTGAAAGAAGAACAGCAGGTTTTTCCCTTGATCCTGCAGCAGTTCTGTCGTGAAGAACAGGCTGCACTTGTGTGGCAGTTCATGTGCAGCGTTCCTTTATTGCTGCTTGAGGATCTACTTCCGTGGACGATCTCCTTTCTTCCACCTGATGAACAAGAGGAAGTTAGACATTGCATAAAAGCAATTGTACCTGACGAAAAATCACTTGAAGAGGTGGTAAATTCTTGGCTTGCTAGCAATGAACAATCCTCCTTTGGGGCCACCAAGAATTCTAGAGGAGCTCAACAAGCCGGCGAATATGCAGACATGAAAAGGTTACTGAAGTCTCATTCGCCCAAAAGGTTTTTAGAAGAATATAAAAGACACATTAAGGCAGATTGCATTCACTCTGATATTGGATACAATCCGGTTGATGGCCTTCCTTTTTGGCATGGAGTCATTAGGAAAGATTTGAAGACAATTCTGGAGGAGCTATATCAACTAAGAAGCGCAAGCAGTTTTTTGAATCTTGATTCAGTAGTTGTCCAGCTAAAATTCTTTGTAGATGTCCTTAATTTCTACAGCAGTGCATTGGAGAAGTTATATCATCCTGTGTTGAATGAACTTTTCAACGGCTGCCTTTACCCCTACAGTGAACAATTTCCAAATGAAAGCCACGTTGAAGGTTTGCAGAGGTTGCTATACTATGAGCCTCAAGATGGAACACCTTTGAGCAAATTTGTTGAGAAGCTTTGCTGGGAACTGGAGTCTTTTCTGGTGGGAATTAACAAGTACTTTGCTTTCCAAGAAACCAAGGTAGTTCCCATTGTAAGGAAGAATTGCGGTCATGATATGCAGCTGCAACTCTTGTATGCAAGCCTTCATATTCTGCCACTTGGGTTGCTAAAGTGCATGACTACTTGGTTTTCGGCTTGCTTATCTGAGGACGAATCCAGGTCCATCCTTAGCAACTTGAAGGAGGGAGATTCTTTAGTCAATAAATCATTTGCATCCCTCTTACATGAGTGGTTCCGCATTGGTCATTCAGGTAAGACCTCTGTTGAAAAGTTTCGTGAGGAGTTGCAGCAAATATTCAAGAGCAGATGCACTTCCTTAAAGCAATTCTATAATACTAGTGGATCTTCCTCCTTAAGTTCCAATATGAAACCTATTGAGGCATCAAACACTAAGCTAATGGAGCCAATCTCTTTGGATAAGGGCAAGAAATCTCTGTCATACTCTTCATCTTGTGCCTCTGACTCTGCCAGGAATTATAGGACGTCATATTCCAGCAGAAACAAACTTCACTTATATTTCACTGGAACAGTGAAAACTTCATATCTCTTTCCTGAAAGTCTTAGTGGAGAGAACCATCCTGGTTATGCTCTTCATGAACCAAAACCAATAGATCTCATATTTTTCTTCCACAAGGCTCTCAAGAAAGATTTGGAATACCTTGTATTTGGCTCAGCTCAGTTGGCTGACAATGCTGATTTTCTCTCAGAGTTTTGCCGGCGATTCCAACTTATACAGTTTCTAAATCAAATACATAGTGAAGCAGAGGAAGAAGTTGCCTTTCCAGCTTTAGAGACCAAAGGAAAACTGCAAAACATTAGCCACTCTTACACGATAGACCACAAATTAGAAGTTGAACTCTTTCACAAGATATCCCTCGTTCTGGATGAGATGTCTATATTGCATGTTTCAGCTTCTGAAGTCGATTCAAATGCAGTGGATAATAAAATGCAGAAGCACCATCAGCTGTGCAGGAGGCTTCATGACATGTGCACATCAACCTGCAAGTTACTGACTGAACATGTTCATCGTGAAGAACTTGAGCTCTGGCCCTTGTTTAAAGAATGCTTCTCCATTGAAGAGCAAGAAAGGATTGTAGGATGCATACTTGGTAGAACAGAAGCAAAAATATTGCAAGATATGTTACCTTGGCTAATGGACGCTTTGACACAAGAAGAACAGCAAGTCATGATTACTCTATGGCGCCAGGTCACACGAAACACGATGTTTGATGAGTGGTTGAGAGAATGGTGGGAGGGATATGAGACAGCTAAGTTGGTAGAGGAGTCCATTGTACCTCCTTCATGGACTGAAGATCCATTGGAGGTTGTCTCTGCCTATCTGTGTGGATCGAGTGAACAAGAAGGAAGATGCTGTAACAAAAGTATCAATTTTCCAGAAAAAGATTCCCATAGTGCTAATACCAAGCCATCGGAAAATAGTGAGGTGGGTTATAAGCCAAAAGGTCCTGGAGGTGACCAATGTATTTCCACTGATACAGAATGTACAAGACTTTGTGATGAAGGTAACAAGAAGAAGTTACAAGAAGTTGAAAATGCCACAAATCAAATTGATGATCTAGGTCACCTTTTGCAAAGAAGCCAGAAATCCAAGTACTGTGAGTGCCTGCTGACACTTAGTCAAGAGGATATGCAGGCTGCAGTAATAAAAATCTCCCGTGACTCTTCCTTGGATCCTCAGAAGAAACCACATATGATCCAGAACCTGATAATGAGCCGTTGGATAGCCAGACAGAACTCTGAATTAACAGTTGCAAGTAATGGGAAAGAATTTCCTGGTCAGCATCCATCCTATCATGACCCTCTTGGAGTAACCTATGGTTGTAAACACTATAAGAGGAACTGTAAGCTTTTTGCTGCCTGTTGCAACCAACTTTACACTTGCATACGCTGCCATGATGAGATGGCTGATCATGAGATAGATAGGAAATCTATAACAGAGATGATGTGCATGAAATGCTTGAAGATGCAGACAGTTGGGCCCACATGCTCAACTGCATCCTGCAGTAACTTTTCCATGGCAAAATACTTTTGCAGGATCTGCAAAATATTTGATAACGAACGGGTCATCTACCATTGTCCTTACTGTAACTTGTGCCGAGTTGGAAAGGGATTGGGTATTGACTACTTCCATTGCATGACGTGTAATGCCTGCATGTCCCGTTCTCTATTGAAGCACACATGCAGAGAGAAATGCTTCATGGATAACTGCCCTATTTGCAATGAAGACATCTTCACCTCAAATTCTCCAGTGAAGTCCCTTCCATGCGGTCATTTGATGCACTCAACATGTTTTGAGGCCTACACCTTTACGAATTATACCTGCCCAATCTGTGGCAAGTCACTCGGGGACATGCAGGTGTATTTTAAAATGTTGGATGCATTTTTGGCTAAACAGGAAACTCCAGACGAGTACGCTGGCCAAACTCAGGTCATACTCTGCAATGACTGTGAGAAGAGAGGAACTGCTCCCTTTCACTGGCTTTACCACAAGTGCCCCTATTGTGGTTCATACAACACCAGGCTTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006873 GO:0006875 GO:0006879 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0010106 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019725 GO:0019787 GO:0019941 GO:0030003 GO:0030163 GO:0031667 GO:0031668 GO:0031669 GO:0032446 GO:0033554 GO:0036211 GO:0042592 GO:0042594 GO:0043161 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0050896 GO:0051603 GO:0051716 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070647 GO:0071496 GO:0071704 GO:0098771 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hemerythrin zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

1276

Amino Acids

145.97

Weight (kDa)

5.72

Isoelectric Point (pI)

49.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hemerythrin	PF01814	43 - 178	1.8e-07	Hemerythrin HHE cation binding domain
zf-CHY	PF05495	1040 - 1108	2.8e-16	CHY zinc finger
zf-RING_2	PF13639	1167 - 1210	2.7e-09	Ring finger domain
zinc_ribbon_6	PF14599	1216 - 1273	5.2e-24	Zinc-ribbon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008770)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18910 AT1G74770 AT1G74770
fragaria_vesca	FvH4_5g15780 FvH4_5g15780 FvH4_5g15780
malus_domestica	MD14G1191800.v1.1
prunus_persica	Prupe.1G314300_v2.0.a1 Prupe.5G184400_v2.0.a1 Prupe.5G184400_v2.0.a1
pyrus_communis	pycom14g15950
rosa_chinensis	RchiOBHm_Chr7g0180341
rosa_laevigata	RLG00000005275
rosa_multiflora	Rmu_sc0003526.1_g000012 Rmu_ssc0000099.1_g000006
rosa_roxburghii	Rroxscaffold_3G00244490 Rroxscaffold_3G00272970
rosa_rugosa	Rorug06G0432400
rosa_samantha	Rh7AG032800 Rh7BG032800 Rh7CG034700 Rh7DG033400
rosa_wichuraiana	Rw7G002740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2730
AarI	CACCTGC	1 cut(s)	3643
AatII	GACGTC	1 cut(s)	1763
Acc36I	ACCTGC	4 cut(s)	508, 2285, 3626, 3643
AccB1I	GGYRCC	1 cut(s)	2478
AccB7I	CCANNNNNTGG	1 cut(s)	3629
AccBSI	CCGCTC	1 cut(s)	94
AccII	CGCG	3 cut(s)	80, 216, 263
AcoI	YGGCCR	1 cut(s)	3715
AcsI	RAATTY	8 cut(s)	431, 482, 721, 769, 1034, 1217, 3065, 3538
AcuI	CTGAAG	4 cut(s)	497, 842, 2214, 2607
AcvI	CACGTG	2 cut(s)	86, 200
AcyI	GRCGYC	3 cut(s)	104, 1760, 2479
AfaI	GTAC	7 cut(s)	340, 690, 1274, 2571, 2782, 2891, 3710
AfiI	CCNNNNNNNGG	9 cut(s)	756, 914, 1199, 1311, 2158, 3629, 3724, 3774, 3800
AflII	CTTAAG	1 cut(s)	1630
AflIII	ACRYGT	6 cut(s)	83, 85, 2262, 2296, 3437, 3584
AhdI	GACNNNNNGTC	1 cut(s)	2913
AhlI	ACTAGT	1 cut(s)	1613
AjiI	CACGTC	1 cut(s)	3438
AjnI	CCWGG	7 cut(s)	1433, 1745, 1855, 2481, 2743, 3070, 3815
AjuI	GAANNNNNNNTTGG	2 cut(s)	444, 476
AleI	CACNNNNGTG	1 cut(s)	571
Alw21I	GWGCWC	4 cut(s)	153, 784, 2270, 2322
Alw26I	GTCTC	5 cut(s)	377, 831, 2063, 2534, 2611
Alw44I	GTGCAC	1 cut(s)	2266
AlwNI	CAGNNNCTG	3 cut(s)	2192, 3001, 3336
Ama87I	CYCGRG	1 cut(s)	3641
AoxI	GGCC	7 cut(s)	144, 759, 916, 2324, 3275, 3594, 3715
ApaI	GGGCCC	1 cut(s)	3279
ApaLI	GTGCAC	1 cut(s)	2266
ApoI	RAATTY	8 cut(s)	431, 482, 721, 769, 1034, 1217, 3065, 3538
ArsI	GACNNNNNNTTYG	4 cut(s)	203, 235, 359, 391
Asp700I	GAANNNNTTC	6 cut(s)	482, 1140, 1548, 2136, 2298, 3491
AspLEI	GCGC	3 cut(s)	139, 992, 2481
AspS9I	GGNCC	6 cut(s)	759, 1437, 2325, 2741, 3275, 3276
AsuHPI	GGTGA	3 cut(s)	2762, 2852, 3523
AsuNHI	GCTAGC	1 cut(s)	734
AvaI	CYCGRG	1 cut(s)	3641
AvaII	GGWCC	2 cut(s)	1437, 2741
BaeGI	GKGCMC	3 cut(s)	2270, 3279, 3794
BaeI	ACNNNNGTAYC	2 cut(s)	2644, 2677
BalI	TGGCCA	1 cut(s)	3717
BamHI	GGATCC	1 cut(s)	2968
BanI	GGYRCC	1 cut(s)	2478
BanII	GRGCYC	4 cut(s)	153, 784, 2322, 3279
BauI	CACGAG	1 cut(s)	167
BbrPI	CACGTG	2 cut(s)	86, 200
BbsI	GAAGAC	2 cut(s)	962, 3528
Bbv12I	GWGCWC	4 cut(s)	153, 784, 2270, 2322
BccI	CCATC	9 cut(s)	308, 908, 1193, 1448, 1860, 2244, 2713, 3091, 3193
BceAI	ACGGC	2 cut(s)	1129, 2997
BcgI	CGANNNNNNTGC	8 cut(s)	97, 131, 530, 564, 2189, 2223, 3622, 3656
BciT130I	CCWGG	7 cut(s)	1435, 1747, 1857, 2483, 2745, 3072, 3817
BciVI	GTATCC	1 cut(s)	893
BclI	TGATCA	1 cut(s)	3205
BcoDI	GTCTC	5 cut(s)	377, 831, 2063, 2534, 2611
BcuI	ACTAGT	1 cut(s)	1613
BfaI	CTAG	7 cut(s)	209, 336, 350, 735, 774, 1614, 2855
BfmI	CTRYAG	7 cut(s)	140, 282, 536, 1060, 1129, 2934, 3298
BfoI	RGCGCY	2 cut(s)	140, 2482
BfrI	CTTAAG	1 cut(s)	1630
BfuAI	ACCTGC	4 cut(s)	508, 2285, 3626, 3643
BfuI	GTATCC	1 cut(s)	893
BglII	AGATCT	1 cut(s)	1888
BlpI	GCTNAGC	1 cut(s)	1948
BmcAI	AGTACT	2 cut(s)	1274, 2891
Bme1390I	CCNGG	7 cut(s)	1435, 1747, 1857, 2483, 2745, 3072, 3817
Bme18I	GGWCC	2 cut(s)	1437, 2741
BmeRI	GACNNNNNGTC	1 cut(s)	2913
BmeT110I	CYCGRG	1 cut(s)	3641
BmgBI	CACGTC	1 cut(s)	3438
BmgT120I	GGNCC	6 cut(s)	759, 1437, 2325, 2741, 3275, 3276
BmiI	GGNNCC	6 cut(s)	760, 1511, 1680, 2480, 2970, 3277
BmrFI	CCNGG	7 cut(s)	1435, 1747, 1857, 2483, 2745, 3072, 3817
BmtI	GCTAGC	1 cut(s)	738
BoxI	GACNNNNGTC	1 cut(s)	86
BpiI	GAAGAC	2 cut(s)	962, 3528
BplI	GAGNNNNNCTC	6 cut(s)	179, 211, 1670, 1702, 2591, 2623
BpmI	CTGGAG	5 cut(s)	986, 1265, 2766, 3528, 3684
Bpu10I	CCTNAGC	1 cut(s)	1445
Bpu1102I	GCTNAGC	1 cut(s)	1948
BpuEI	CTTGAG	5 cut(s)	239, 629, 1179, 1901, 2336
BsaAI	YACGTR	2 cut(s)	86, 200
BsaHI	GRCGYC	3 cut(s)	104, 1760, 2479
BsaI	GGTCTC	2 cut(s)	377, 2063
BsaJI	CCNNGG	6 cut(s)	183, 623, 1293, 2423, 2964, 3312
BsaWI	WCCGGW	1 cut(s)	907
BsaXI	ACNNNNNCTCC	2 cut(s)	189, 219
Bsc4I	CCNNNNNNNGG	9 cut(s)	756, 914, 1199, 1311, 2158, 3629, 3724, 3774, 3800
Bse118I	RCCGGY	2 cut(s)	791, 1992
Bse1I	ACTGG	4 cut(s)	1248, 1804, 3545, 3779
Bse3DI	GCAATG	5 cut(s)	670, 745, 3427, 3523, 3745
BseBI	CCWGG	7 cut(s)	1435, 1747, 1857, 2483, 2745, 3072, 3817
BseDI	CCNNGG	6 cut(s)	183, 623, 1293, 2423, 2964, 3312
BseLI	CCNNNNNNNGG	9 cut(s)	756, 914, 1199, 1311, 2158, 3629, 3724, 3774, 3800
BseMI	GCAATG	5 cut(s)	670, 745, 3427, 3523, 3745
BseMII	CTCAG	7 cut(s)	113, 1413, 1962, 1967, 1995, 2987, 3737
BseNI	ACTGG	4 cut(s)	1248, 1804, 3545, 3779
BseRI	GAGGAG	7 cut(s)	23, 26, 143, 792, 983, 1571, 2573
BseSI	GKGCMC	3 cut(s)	2270, 3279, 3794
BseYI	CCCAGC	3 cut(s)	57, 492, 1236
BsgI	GTGCAG	3 cut(s)	549, 607, 2266
Bsh1236I	CGCG	3 cut(s)	80, 216, 263
BshFI	GGCC	7 cut(s)	146, 761, 918, 2326, 3277, 3596, 3717
BshNI	GGYRCC	1 cut(s)	2478
BsiHKAI	GWGCWC	4 cut(s)	153, 784, 2270, 2322
BsiHKCI	CYCGRG	1 cut(s)	3641
BsiSI	CCGG	4 cut(s)	62, 792, 908, 1993
BslFI	GGGAC	6 cut(s)	74, 256, 484, 3439, 3538, 3659
BslI	CCNNNNNNNGG	9 cut(s)	756, 914, 1199, 1311, 2158, 3629, 3724, 3774, 3800
BsmAI	GTCTC	5 cut(s)	377, 831, 2063, 2534, 2611
BsmFI	GGGAC	6 cut(s)	74, 256, 484, 3439, 3538, 3659
BsmI	GAATGC	2 cut(s)	882, 2345
BsnI	GGCC	7 cut(s)	146, 761, 918, 2326, 3277, 3596, 3717
Bso31I	GGTCTC	2 cut(s)	377, 2063
BsoBI	CYCGRG	1 cut(s)	3641
Bsp120I	GGGCCC	1 cut(s)	3275
Bsp1286I	GDGCHC	6 cut(s)	153, 784, 2270, 2322, 3279, 3794
Bsp1407I	TGTACA	1 cut(s)	2780
Bsp1720I	GCTNAGC	1 cut(s)	1948
Bsp19I	CCATGG	1 cut(s)	3312
BspANI	GGCC	7 cut(s)	146, 761, 918, 2326, 3277, 3596, 3717
BspCNI	CTCAG	7 cut(s)	112, 1414, 1961, 1966, 1994, 2986, 3736
BspFNI	CGCG	3 cut(s)	80, 216, 263
BspHI	TCATGA	6 cut(s)	1327, 1870, 2257, 2463, 3091, 3208
BspLI	GGNNCC	6 cut(s)	760, 1511, 1680, 2480, 2970, 3277
BspMAI	CTGCAG	5 cut(s)	144, 286, 540, 2938, 3302
BspMI	ACCTGC	4 cut(s)	508, 2285, 3626, 3643
BspOI	GCTAGC	1 cut(s)	738
BspT107I	GGYRCC	1 cut(s)	2478
BspTI	CTTAAG	1 cut(s)	1630
BspTNI	GGTCTC	2 cut(s)	377, 2063
BsrBI	CCGCTC	1 cut(s)	94
BsrDI	GCAATG	5 cut(s)	670, 745, 3427, 3523, 3745
BsrFI	RCCGGY	2 cut(s)	791, 1992
BsrGI	TGTACA	1 cut(s)	2780
BsrI	ACTGG	4 cut(s)	1248, 1804, 3545, 3779
BssAI	RCCGGY	2 cut(s)	791, 1992
BssECI	CCNNGG	6 cut(s)	183, 623, 1293, 2423, 2964, 3312
BssNI	GRCGYC	3 cut(s)	104, 1760, 2479
BssSI	CACGAG	1 cut(s)	167
BssT1I	CCWWGG	5 cut(s)	183, 1293, 2423, 2964, 3312
Bst2BI	CACGAG	1 cut(s)	167
Bst2UI	CCWGG	7 cut(s)	1435, 1747, 1857, 2483, 2745, 3072, 3817
Bst6I	CTCTTC	2 cut(s)	380, 705
BstACI	GRCGYC	3 cut(s)	104, 1760, 2479
BstAFI	CTTAAG	1 cut(s)	1630
BstAPI	GCANNNNNTGC	4 cut(s)	1177, 3186, 3252, 3336
BstAUI	TGTACA	1 cut(s)	2780
BstBAI	YACGTR	2 cut(s)	86, 200
BstDSI	CCRYGG	2 cut(s)	623, 3312
BstEII	GGTNACC	2 cut(s)	2750, 2858
BstFNI	CGCG	3 cut(s)	80, 216, 263
BstH2I	RGCGCY	2 cut(s)	140, 2482
BstHHI	GCGC	3 cut(s)	139, 992, 2481
BstMAI	GTCTC	5 cut(s)	377, 831, 2063, 2534, 2611
BstNI	CCWGG	7 cut(s)	1435, 1747, 1857, 2483, 2745, 3072, 3817
BstNSI	RCATGY	8 cut(s)	2183, 2266, 2300, 3285, 3454, 3480, 3588, 3652
BstPAI	GACNNNNGTC	1 cut(s)	86
BstPI	GGTNACC	2 cut(s)	2750, 2858
BstSCI	CCNGG	7 cut(s)	1433, 1745, 1855, 2481, 2743, 3070, 3815
BstSFI	CTRYAG	7 cut(s)	140, 282, 536, 1060, 1129, 2934, 3298
BstSLI	GKGCMC	3 cut(s)	2270, 3279, 3794
BstUI	CGCG	3 cut(s)	80, 216, 263
BstV2I	GAAGAC	2 cut(s)	962, 3528
BstX2I	RGATCY	6 cut(s)	613, 1619, 1888, 2590, 2968, 3333
BstYI	RGATCY	6 cut(s)	613, 1619, 1888, 2590, 2968, 3333
BsuI	GTATCC	1 cut(s)	893
BsuRI	GGCC	7 cut(s)	146, 761, 918, 2326, 3277, 3596, 3717
BtgI	CCRYGG	2 cut(s)	623, 3312
BtrI	CACGTC	1 cut(s)	3438
BtsI	GCAGTG	3 cut(s)	274, 1072, 2217
BtsIMutI	CAGTG	9 cut(s)	274, 1072, 1138, 1797, 1812, 2217, 2766, 3552, 3772
BveI	ACCTGC	4 cut(s)	508, 2285, 3626, 3643
CaiI	CAGNNNCTG	3 cut(s)	2192, 3001, 3336
CciI	TCATGA	6 cut(s)	1327, 1870, 2257, 2463, 3091, 3208
CfoI	GCGC	3 cut(s)	139, 992, 2481
Cfr10I	RCCGGY	2 cut(s)	791, 1992
Cfr13I	GGNCC	6 cut(s)	759, 1437, 2325, 2741, 3275, 3276
CseI	GACGC	3 cut(s)	112, 481, 2444
Csp6I	GTAC	7 cut(s)	339, 689, 1273, 2570, 2781, 2890, 3709
CspCI	CAANNNNNGTGG	4 cut(s)	1362, 1397, 2193, 2228
CviQI	GTAC	7 cut(s)	339, 689, 1273, 2570, 2781, 2890, 3709
DinI	GGCGCC	1 cut(s)	2480
DraI	TTTAAA	2 cut(s)	2335, 3664
DriI	GACNNNNNGTC	1 cut(s)	2913
EaeI	YGGCCR	1 cut(s)	3715
Eam1104I	CTCTTC	7 cut(s)	380, 705, 1726, 1872, 2349, 2965, 3746
Eam1105I	GACNNNNNGTC	1 cut(s)	2913
EarI	CTCTTC	7 cut(s)	380, 705, 1726, 1872, 2349, 2965, 3746
EciI	GGCGGA	1 cut(s)	152
Ecl136II	GAGCTC	3 cut(s)	151, 782, 2320
Eco130I	CCWWGG	5 cut(s)	183, 1293, 2423, 2964, 3312
Eco147I	AGGCCT	1 cut(s)	3596
Eco24I	GRGCYC	4 cut(s)	153, 784, 2322, 3279
Eco31I	GGTCTC	2 cut(s)	377, 2063
Eco32I	GATATC	1 cut(s)	2148
Eco47I	GGWCC	2 cut(s)	1437, 2741
Eco53kI	GAGCTC	3 cut(s)	151, 782, 2320
Eco57I	CTGAAG	4 cut(s)	497, 842, 2214, 2607
Eco72I	CACGTG	2 cut(s)	86, 200
Eco88I	CYCGRG	1 cut(s)	3641
Eco91I	GGTNACC	2 cut(s)	2750, 2858
EcoICRI	GAGCTC	3 cut(s)	151, 782, 2320
EcoO109I	RGGNCCY	1 cut(s)	2741
EcoO65I	GGTNACC	2 cut(s)	2750, 2858
EcoRI	GAATTC	2 cut(s)	482, 769
EcoRII	CCWGG	7 cut(s)	1433, 1745, 1855, 2481, 2743, 3070, 3815
EcoRV	GATATC	1 cut(s)	2148
EcoT14I	CCWWGG	5 cut(s)	183, 1293, 2423, 2964, 3312
EcoT22I	ATGCAT	2 cut(s)	2381, 3679
EcoT38I	GRGCYC	4 cut(s)	153, 784, 2322, 3279
EgeI	GGCGCC	1 cut(s)	2480
EheI	GGCGCC	1 cut(s)	2480
ErhI	CCWWGG	5 cut(s)	183, 1293, 2423, 2964, 3312
FaqI	GGGAC	6 cut(s)	74, 256, 484, 3439, 3538, 3659
FauI	CCCGC	1 cut(s)	99
FauNDI	CATATG	3 cut(s)	357, 498, 2988
FbaI	TGATCA	1 cut(s)	3205
FriOI	GRGCYC	4 cut(s)	153, 784, 2322, 3279
FspBI	CTAG	7 cut(s)	209, 336, 350, 735, 774, 1614, 2855
GlaI	GCGC	3 cut(s)	138, 991, 2480
GsaI	CCCAGC	3 cut(s)	61, 496, 1240
GsuI	CTGGAG	5 cut(s)	986, 1265, 2766, 3528, 3684
HaeII	RGCGCY	2 cut(s)	140, 2482
HaeIII	GGCC	7 cut(s)	146, 761, 918, 2326, 3277, 3596, 3717
HapII	CCGG	4 cut(s)	62, 792, 908, 1993
HgaI	GACGC	3 cut(s)	112, 481, 2444
HhaI	GCGC	3 cut(s)	139, 992, 2481
Hin1I	GRCGYC	3 cut(s)	104, 1760, 2479
Hin6I	GCGC	3 cut(s)	137, 990, 2479
HinP1I	GCGC	3 cut(s)	137, 990, 2479
HindIII	AAGCTT	2 cut(s)	1229, 3143
HpaII	CCGG	4 cut(s)	62, 792, 908, 1993
HphI	GGTGA	3 cut(s)	2762, 2852, 3523
Hpy166II	GTNNAC	5 cut(s)	627, 1136, 2268, 2633, 3122
Hpy188I	TCNGA	9 cut(s)	102, 892, 1423, 1738, 1984, 2194, 2710, 2976, 3037
Hpy8I	GTNNAC	5 cut(s)	627, 1136, 2268, 2633, 3122
Hpy99I	CGWCG	2 cut(s)	14, 17
HpyCH4IV	ACGT	5 cut(s)	85, 199, 1158, 1760, 3437
HpySE526I	ACGT	5 cut(s)	85, 199, 1158, 1760, 3437
Hsp92I	GRCGYC	3 cut(s)	104, 1760, 2479
HspAI	GCGC	3 cut(s)	137, 990, 2479
KasI	GGCGCC	1 cut(s)	2478
KroI	GCCGGC	2 cut(s)	791, 1992
KroNI	GCCGGC	2 cut(s)	793, 1994
Ksp22I	TGATCA	1 cut(s)	3205
LguI	GCTCTTC	2 cut(s)	1872, 2349
LmnI	GCTCC	5 cut(s)	156, 779, 970, 1678, 3769
MaeI	CTAG	7 cut(s)	209, 336, 350, 735, 774, 1614, 2855
MaeII	ACGT	5 cut(s)	85, 199, 1158, 1760, 3437
MbiI	CCGCTC	1 cut(s)	94
MfeI	CAATTG	1 cut(s)	684
MflI	RGATCY	6 cut(s)	613, 1619, 1888, 2590, 2968, 3333
MhlI	GDGCHC	6 cut(s)	153, 784, 2270, 2322, 3279, 3794
MlsI	TGGCCA	1 cut(s)	3717
MluNI	TGGCCA	1 cut(s)	3717
Mly113I	GGCGCC	1 cut(s)	2479
MlyI	GAGTC	8 cut(s)	74, 278, 374, 945, 1256, 1733, 2570, 2951
MmeI	TCCRAC	4 cut(s)	2026, 2996, 3379, 3651
Mox20I	TGGCCA	1 cut(s)	3717
Mph1103I	ATGCAT	2 cut(s)	2381, 3679
MroNI	GCCGGC	2 cut(s)	791, 1992
MroXI	GAANNNNTTC	6 cut(s)	482, 1140, 1548, 2136, 2298, 3491
MscI	TGGCCA	1 cut(s)	3717
MseI	TTAA	8 cut(s)	870, 1053, 1266, 1595, 1631, 2334, 3041, 3663
MslI	CAYNNNNRTG	2 cut(s)	571, 3653
Msp20I	TGGCCA	1 cut(s)	3717
MspA1I	CMGCKG	2 cut(s)	1339, 2242
MspCI	CTTAAG	1 cut(s)	1630
MspI	CCGG	4 cut(s)	62, 792, 908, 1993
MspR9I	CCNGG	7 cut(s)	1435, 1747, 1857, 2483, 2745, 3072, 3817
MunI	CAATTG	1 cut(s)	684
Mva1269I	GAATGC	2 cut(s)	882, 2345
MvaI	CCWGG	7 cut(s)	1435, 1747, 1857, 2483, 2745, 3072, 3817
MvnI	CGCG	3 cut(s)	80, 216, 263
NaeI	GCCGGC	2 cut(s)	793, 1994
NarI	GGCGCC	1 cut(s)	2479
NcoI	CCATGG	1 cut(s)	3312
NdeI	CATATG	3 cut(s)	357, 498, 2988
NgoMIV	GCCGGC	2 cut(s)	791, 1992
NheI	GCTAGC	1 cut(s)	734
NlaIV	GGNNCC	6 cut(s)	760, 1511, 1680, 2480, 2970, 3277
NmeAIII	GCCGAG	2 cut(s)	172, 3419
NmuCI	GTSAC	7 cut(s)	169, 200, 2485, 2750, 2858, 2954, 3636
NsiI	ATGCAT	2 cut(s)	2381, 3679
NspI	RCATGY	8 cut(s)	2183, 2266, 2300, 3285, 3454, 3480, 3588, 3652
OliI	CACNNNNGTG	1 cut(s)	571
PagI	TCATGA	6 cut(s)	1327, 1870, 2257, 2463, 3091, 3208
PaqCI	CACCTGC	1 cut(s)	3643
PceI	AGGCCT	1 cut(s)	3596
PciI	ACATGT	3 cut(s)	2262, 2296, 3584
PciSI	GCTCTTC	2 cut(s)	1872, 2349
PcsI	WCGNNNNNNNCGW	1 cut(s)	375
PctI	GAATGC	2 cut(s)	882, 2345
PdiI	GCCGGC	2 cut(s)	793, 1994
PdmI	GAANNNNTTC	6 cut(s)	482, 1140, 1548, 2136, 2298, 3491
PfeI	GAWTC	8 cut(s)	17, 1006, 1013, 1430, 1466, 1998, 2201, 2681
PflMI	CCANNNNNTGG	1 cut(s)	3629
PfoI	TCCNGGA	1 cut(s)	2743
PleI	GAGTC	8 cut(s)	73, 277, 374, 944, 1255, 1733, 2569, 2951
PluTI	GGCGCC	1 cut(s)	2482
PmaCI	CACGTG	2 cut(s)	86, 200
PmlI	CACGTG	2 cut(s)	86, 200
PpsI	GAGTC	8 cut(s)	73, 277, 374, 944, 1255, 1733, 2569, 2951
Ppu21I	YACGTR	2 cut(s)	86, 200
PpuMI	RGGWCCY	1 cut(s)	2741
PscI	ACATGT	3 cut(s)	2262, 2296, 3584
PshAI	GACNNNNGTC	1 cut(s)	86
PsiI	TTATAA	1 cut(s)	2730
Psp124BI	GAGCTC	3 cut(s)	153, 784, 2322
Psp5II	RGGWCCY	1 cut(s)	2741
Psp6I	CCWGG	7 cut(s)	1433, 1745, 1855, 2481, 2743, 3070, 3815
PspCI	CACGTG	2 cut(s)	86, 200
PspEI	GGTNACC	2 cut(s)	2750, 2858
PspFI	CCCAGC	3 cut(s)	57, 492, 1236
PspGI	CCWGG	7 cut(s)	1433, 1745, 1855, 2481, 2743, 3070, 3815
PspN4I	GGNNCC	6 cut(s)	760, 1511, 1680, 2480, 2970, 3277
PspOMI	GGGCCC	1 cut(s)	3275
PspPI	GGNCC	6 cut(s)	759, 1437, 2325, 2741, 3275, 3276
PspPPI	RGGWCCY	1 cut(s)	2741
PstI	CTGCAG	5 cut(s)	144, 286, 540, 2938, 3302
PstNI	CAGNNNCTG	3 cut(s)	2192, 3001, 3336
PsuI	RGATCY	6 cut(s)	613, 1619, 1888, 2590, 2968, 3333
PvuII	CAGCTG	2 cut(s)	1339, 2242
RsaI	GTAC	7 cut(s)	340, 690, 1274, 2571, 2782, 2891, 3710
RsaNI	GTAC	7 cut(s)	339, 689, 1273, 2570, 2781, 2890, 3709
RseI	CAYNNNNRTG	2 cut(s)	571, 3653
SacI	GAGCTC	3 cut(s)	153, 784, 2322
SapI	GCTCTTC	2 cut(s)	1872, 2349
SaqAI	TTAA	8 cut(s)	870, 1053, 1266, 1595, 1631, 2334, 3041, 3663
Sau96I	GGNCC	6 cut(s)	759, 1437, 2325, 2741, 3275, 3276
ScaI	AGTACT	2 cut(s)	1274, 2891
SchI	GAGTC	8 cut(s)	74, 278, 374, 945, 1256, 1733, 2570, 2951
ScrFI	CCNGG	7 cut(s)	1435, 1747, 1857, 2483, 2745, 3072, 3817
SduI	GDGCHC	6 cut(s)	153, 784, 2270, 2322, 3279, 3794
SfcI	CTRYAG	7 cut(s)	140, 282, 536, 1060, 1129, 2934, 3298
SfoI	GGCGCC	1 cut(s)	2480
SinI	GGWCC	2 cut(s)	1437, 2741
SmiMI	CAYNNNNRTG	2 cut(s)	571, 3653
SmlI	CTYRAG	6 cut(s)	254, 608, 1194, 1630, 1916, 2315
SmoI	CTYRAG	6 cut(s)	254, 608, 1194, 1630, 1916, 2315
SpeI	ACTAGT	1 cut(s)	1613
SseBI	AGGCCT	1 cut(s)	3596
SspDI	GGCGCC	1 cut(s)	2478
SspI	AATATT	3 cut(s)	1572, 2406, 3345
SspMI	CTAG	7 cut(s)	209, 336, 350, 735, 774, 1614, 2855
SstI	GAGCTC	3 cut(s)	153, 784, 2322
StuI	AGGCCT	1 cut(s)	3596
StyD4I	CCNGG	7 cut(s)	1433, 1745, 1855, 2481, 2743, 3070, 3815
StyI	CCWWGG	5 cut(s)	183, 1293, 2423, 2964, 3312
TaiI	ACGT	5 cut(s)	88, 202, 1161, 1763, 3440
TaqI	TCGA	4 cut(s)	156, 378, 2199, 2627
TatI	WGTACW	4 cut(s)	338, 1272, 2780, 2889
TauI	GCSGC	3 cut(s)	9, 182, 241
TfiI	GAWTC	8 cut(s)	17, 1006, 1013, 1430, 1466, 1998, 2201, 2681
Tru1I	TTAA	8 cut(s)	870, 1053, 1266, 1595, 1631, 2334, 3041, 3663
Tru9I	TTAA	8 cut(s)	870, 1053, 1266, 1595, 1631, 2334, 3041, 3663
TscAI	CASTG	9 cut(s)	281, 1072, 1138, 1804, 1812, 2217, 2773, 3552, 3779
TseFI	GTSAC	7 cut(s)	169, 200, 2485, 2750, 2858, 2954, 3636
Tsp45I	GTSAC	7 cut(s)	169, 200, 2485, 2750, 2858, 2954, 3636
TspGWI	ACGGA	1 cut(s)	612
TspRI	CASTG	9 cut(s)	281, 1072, 1138, 1804, 1812, 2217, 2773, 3552, 3779
Van91I	CCANNNNNTGG	1 cut(s)	3629
Vha464I	CTTAAG	1 cut(s)	1630
VneI	GTGCAC	1 cut(s)	2266
VpaK11BI	GGWCC	2 cut(s)	1437, 2741
XapI	RAATTY	8 cut(s)	431, 482, 721, 769, 1034, 1217, 3065, 3538
XbaI	TCTAGA	1 cut(s)	773
XceI	RCATGY	8 cut(s)	2183, 2266, 2300, 3285, 3454, 3480, 3588, 3652
XcmI	CCANNNNNNNNNTGG	1 cut(s)	181
XmnI	GAANNNNTTC	6 cut(s)	482, 1140, 1548, 2136, 2298, 3491
XspI	CTAG	7 cut(s)	209, 336, 350, 735, 774, 1614, 2855
ZraI	GACGTC	1 cut(s)	1761
ZrmI	AGTACT	2 cut(s)	1274, 2891
Zsp2I	ATGCAT	2 cut(s)	2381, 3679

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.