Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001960

Physical Location & Seq

Genomic Coordinates Forward (+)

28743234 .. 28745473

Gene Structure 2240 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1197300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGTTGGAAGGTCCTTCCTATCTTGTGTCAAGGGAGTTGCCCAGCTCTTGTGAGCAAGAGAGCAAATGGATTTACAATACTCACCGTGTTATGGAGCTCTCAAACAATAAGCGCCCCATTGAAGACGATGATGAAATCACATTGAGGAAGGTATGCAAGCTATCGGATGCTCTTCTGGGAGGGGATGTGATTATGGATCTGAATGGTCTTCCCCCTGCCAAGGACCAGTCAAATGACGGGCATCAGGGAGGTGGCAATTCTGATTCAAGTTCACTGATCCACCAACTTGGCCGGGATATCTCAATCAACTGTCTCCTTCGCTGCTCGAGGTCTGATTATGGCTCTATTGCCTCGCTGAATAAGAACTTCCGCTCTCTGATTCGGAGTGGGGAGCTGTACACACTGAGGCGGAAAGTGGGCATTGTTGAACATTGGGTTTACTTCTCTTGCAGCCTCCTGGAATGGGAGGCATTTGATCCAGATCTCCGACGTTGGATGCATTTACCTAGAATGGCGTCAAACGAGTGTTTCATGTGTTCAGACAAGGAGTCGTTAGCCGTTGGTACGGAACTTCTTGTTTTTGGAAAGGAAATAACGTCCCATGTTGTTTATAGATACAGCATTTTGACCAACACATGGTCATCGGGCACTGAGATGAATACACCTAGATGCTTGTTCGGTTCTGCAAGCCGTGGGGAAATTGCAATTCTCGCAGGTGGTTGCGATCCACGTGGCAATATCTTGAACTCTGCTGAACTTTATAATTCCGAAACAGGAACTTGGCTGACTCTTCCTAGCATGAATAAACCTAGAAAAATGTGCTCTGGGGTATTTATGGATGGGAAGTTTTATGTCATTGGTGGGATAGGAGTGGGCGACCAAAAGCAACTTACGTGTGGAGAGGTGTACGATTTGGGGAAGGGGACTTGGACTGAGATACCTAACATGTTCCCTGGAAGAAACGCTGGGGCACCTGAGGCACCTGCTGCAGCTGCGGCACCTCCTCTTTTGGCAGTTGTAAATAACATATTGTATGCTGCAGATTATGCAGAACAGGAGGTTAGGAGATATGATAAGGAGAGGAACATGTGGATCACTGTTGGGAGTTTGCCTGAGCGTGCAGCCTCGATGAATGGTTGGGGAATAGCATTTAGGGCATGTGGAGATCGGTTAATTGTAATTGGTGGACCTAGGGCCTTAGGCGGAGGGACAATTGAACTTAATTCTTGGGTCCCTGATGGAGGCCCACCACAGTGGAACCTGCTTGCTAGAAAACCTTCTGGCAGCTTTGTGTATAATTGTGCGGTGATGGGATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0030162 GO:0030163 GO:0031461 GO:0031625 GO:0032991 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0050789 GO:0051171 GO:0051246 GO:0051603 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box Kelch_1

Protein Analysis

440

Amino Acids

48.23

Weight (kDa)

5.81

Isoelectric Point (pI)

50.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_ATRN-LZTR1	PF24981	148 - 315	5.2e-08	Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7	PF24681	185 - 396	7.9e-09	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin	PF13964	200 - 316	4.1e-10	Calicin, beta-propeller domain
Kelch_1	PF01344	222 - 268	1.3e-08	Kelch motif
Kelch_FKB95	PF25210	247 - 314	6.2e-09	FKB95, Kelch-repeats domain
Kelch_1	PF01344	270 - 316	1.6e-10	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001960)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74510 AT1G74510 AT1G74510
fragaria_vesca	FvH4_5g15231 FvH4_5g15231
malus_domestica	MD14G1197300.v1.1
prunus_persica	Prupe.5G189400_v2.0.a1 Prupe.5G189400_v2.0.a1 Prupe.5G189400_v2.0.a1 Prupe.5G189400_v2.0.a1 Prupe.5G189400_v2.0.a1 Prupe.5G189400_v2.0.a1 Prupe.5G189400_v2.0.a1 Prupe.5G189400_v2.0.a1 Prupe.5G189400_v2.0.a1
pyrus_communis	pycom06g16900 pycom14g16390
rosa_chinensis	RchiOBHm_Chr1g0373971 RchiOBHm_Chr1g0373981 RchiOBHm_Chr7g0180901
rosa_laevigata	RLG00000005225 RLG00000026789 RLG00000026790
rosa_multiflora	Rmu_sc0002740.1_g000003 Rmu_sc0004056.1_g000009 Rmu_sc0005304.1_g000003 Rmu_sc0006924.1_g000003
rosa_roxburghii	Rroxscaffold_3G00272420 Rroxscaffold_4G00283850
rosa_rugosa	Rorug06G0437100 Rorug06G0437100 Rorug06G0437100 Rorug06G0437100 Rorug06G0437200.1 Rorug06G0437300 Rorug06G0437400 Rorug06G0437500 Rorug06G0437600 Rorug06G0437700
rosa_samantha	Rh7AG038800 Rh7BG038300 Rh7CG039900 Rh7DG038400
rosa_wichuraiana	Rw1G034480 Rw7G003180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	762
AarI	CACCTGC	2 cut(s)	704, 991
Acc36I	ACCTGC	3 cut(s)	704, 991, 1269
AccB1I	GGYRCC	3 cut(s)	970, 979, 997
AccB7I	CCANNNNNTGG	1 cut(s)	636
AccBSI	CCGCTC	1 cut(s)	372
AciI	CCGC	5 cut(s)	370, 409, 995, 1203, 1304
AclWI	GGATC	5 cut(s)	204, 271, 470, 719, 1100
AcoI	YGGCCR	1 cut(s)	289
AcvI	CACGTG	1 cut(s)	731
AcyI	GRCGYC	1 cut(s)	515
AfaI	GTAC	3 cut(s)	398, 565, 908
AfiI	CCNNNNNNNGG	5 cut(s)	91, 220, 463, 636, 1241
AflIII	ACRYGT	3 cut(s)	893, 945, 1086
AgsI	TTSAA	5 cut(s)	122, 267, 428, 745, 1217
AhdI	GACNNNNNGTC	1 cut(s)	547
AjnI	CCWGG	2 cut(s)	456, 952
AleI	CACNNNNGTG	1 cut(s)	1252
AluBI	AGCT	6 cut(s)	45, 97, 160, 394, 992, 1287
AluI	AGCT	6 cut(s)	45, 97, 160, 394, 992, 1287
Alw21I	GWGCWC	2 cut(s)	99, 824
Alw26I	GTCTC	1 cut(s)	317
AlwI	GGATC	5 cut(s)	204, 271, 470, 719, 1100
Ama87I	CYCGRG	1 cut(s)	325
AoxI	GGCC	3 cut(s)	289, 1194, 1243
ApeKI	GCWGC	8 cut(s)	321, 450, 986, 989, 992, 1037, 1121, 1284
Asp700I	GAANNNNTTC	1 cut(s)	1276
AspA2I	CCTAGG	1 cut(s)	1190
AspLEI	GCGC	1 cut(s)	114
AspS9I	GGNCC	6 cut(s)	11, 223, 1187, 1194, 1231, 1244
AsuC2I	CCSGG	1 cut(s)	293
AsuHPI	GGTGA	2 cut(s)	74, 1318
AvaI	CYCGRG	1 cut(s)	325
AvaII	GGWCC	4 cut(s)	11, 223, 1187, 1231
AvrII	CCTAGG	1 cut(s)	1190
AxyI	CCTNAGG	2 cut(s)	975, 1198
BaeGI	GKGCMC	2 cut(s)	650, 973
BanI	GGYRCC	3 cut(s)	970, 979, 997
BanII	GRGCYC	1 cut(s)	99
BbrPI	CACGTG	1 cut(s)	731
BbsI	GAAGAC	2 cut(s)	129, 200
Bbv12I	GWGCWC	2 cut(s)	99, 824
BbvI	GCAGC	8 cut(s)	308, 462, 973, 979, 1001, 1024, 1133, 1296
BccI	CCATC	3 cut(s)	833, 1232, 1303
BceAI	ACGGC	2 cut(s)	542, 675
BciT130I	CCWGG	2 cut(s)	458, 954
BcnI	CCSGG	1 cut(s)	293
BcoDI	GTCTC	1 cut(s)	317
BfaI	CTAG	6 cut(s)	507, 666, 795, 810, 1191, 1269
BfmI	CTRYAG	2 cut(s)	987, 1038
BfoI	RGCGCY	1 cut(s)	115
BfuAI	ACCTGC	3 cut(s)	704, 991, 1269
BglII	AGATCT	1 cut(s)	481
BisI	GCNGC	9 cut(s)	322, 451, 987, 990, 993, 996, 1038, 1122, 1285
BlnI	CCTAGG	1 cut(s)	1190
BlsI	GCNGC	9 cut(s)	323, 452, 988, 991, 994, 997, 1039, 1123, 1286
Bme1390I	CCNGG	3 cut(s)	293, 458, 954
Bme18I	GGWCC	4 cut(s)	11, 223, 1187, 1231
BmeRI	GACNNNNNGTC	1 cut(s)	547
BmeT110I	CYCGRG	1 cut(s)	325
BmgT120I	GGNCC	6 cut(s)	11, 223, 1187, 1194, 1231, 1244
BmiI	GGNNCC	6 cut(s)	972, 981, 999, 1232, 1233, 1259
BmrFI	CCNGG	3 cut(s)	293, 458, 954
BmsI	GCATC	5 cut(s)	157, 250, 486, 659, 1304
BpiI	GAAGAC	2 cut(s)	129, 200
Bpu10I	CCTNAGC	1 cut(s)	1113
BpuMI	CCSGG	1 cut(s)	293
BsaAI	YACGTR	2 cut(s)	731, 894
BsaBI	GATNNNNATC	1 cut(s)	480
BsaHI	GRCGYC	1 cut(s)	515
BsaJI	CCNNGG	4 cut(s)	219, 691, 952, 1190
BsaXI	ACNNNNNCTCC	2 cut(s)	171, 201
Bsc4I	CCNNNNNNNGG	5 cut(s)	91, 220, 463, 636, 1241
Bse1I	ACTGG	1 cut(s)	226
Bse21I	CCTNAGG	2 cut(s)	975, 1198
Bse8I	GATNNNNATC	1 cut(s)	480
BseBI	CCWGG	2 cut(s)	458, 954
BseDI	CCNNGG	4 cut(s)	219, 691, 952, 1190
BseGI	GGATG	5 cut(s)	172, 190, 501, 844, 1319
BseJI	GATNNNNATC	1 cut(s)	480
BseLI	CCNNNNNNNGG	5 cut(s)	91, 220, 463, 636, 1241
BseMII	CTCAG	5 cut(s)	395, 642, 924, 966, 1104
BseNI	ACTGG	1 cut(s)	226
BseRI	GAGGAG	1 cut(s)	993
BseSI	GKGCMC	2 cut(s)	650, 973
BseXI	GCAGC	8 cut(s)	308, 462, 973, 979, 1001, 1024, 1133, 1296
BseYI	CCCAGC	2 cut(s)	41, 965
BsgI	GTGCAG	1 cut(s)	1140
BshFI	GGCC	3 cut(s)	291, 1196, 1245
BshNI	GGYRCC	3 cut(s)	970, 979, 997
BsiHKAI	GWGCWC	2 cut(s)	99, 824
BsiHKCI	CYCGRG	1 cut(s)	325
BsiSI	CCGG	1 cut(s)	292
BslFI	GGGAC	4 cut(s)	583, 937, 1217, 1222
BslI	CCNNNNNNNGG	5 cut(s)	91, 220, 463, 636, 1241
BsmAI	GTCTC	1 cut(s)	317
BsmFI	GGGAC	4 cut(s)	583, 937, 1217, 1222
BsnI	GGCC	3 cut(s)	291, 1196, 1245
BsoBI	CYCGRG	1 cut(s)	325
Bsp1286I	GDGCHC	4 cut(s)	99, 650, 824, 973
Bsp1407I	TGTACA	1 cut(s)	396
Bsp143I	GATC	7 cut(s)	196, 276, 475, 481, 724, 1092, 1165
BspACI	CCGC	5 cut(s)	370, 409, 995, 1203, 1304
BspANI	GGCC	3 cut(s)	291, 1196, 1245
BspCNI	CTCAG	5 cut(s)	396, 643, 925, 967, 1105
BspLI	GGNNCC	6 cut(s)	972, 981, 999, 1232, 1233, 1259
BspMAI	CTGCAG	2 cut(s)	991, 1042
BspMI	ACCTGC	3 cut(s)	704, 991, 1269
BspPI	GGATC	5 cut(s)	204, 271, 470, 719, 1100
BspQI	GCTCTTC	1 cut(s)	177
BspT107I	GGYRCC	3 cut(s)	970, 979, 997
BsrBI	CCGCTC	1 cut(s)	372
BsrGI	TGTACA	1 cut(s)	396
BsrI	ACTGG	1 cut(s)	226
BssECI	CCNNGG	4 cut(s)	219, 691, 952, 1190
BssMI	GATC	7 cut(s)	196, 276, 475, 481, 724, 1092, 1165
BssNI	GRCGYC	1 cut(s)	515
BssT1I	CCWWGG	2 cut(s)	219, 1190
Bst2UI	CCWGG	2 cut(s)	458, 954
Bst4CI	ACNGT	4 cut(s)	86, 311, 1099, 1254
Bst6I	CTCTTC	2 cut(s)	177, 795
BstACI	GRCGYC	1 cut(s)	515
BstAPI	GCANNNNNTGC	2 cut(s)	986, 1046
BstAUI	TGTACA	1 cut(s)	396
BstBAI	YACGTR	2 cut(s)	731, 894
BstC8I	GCNNGC	4 cut(s)	158, 688, 1119, 1266
BstDEI	CTNAG	6 cut(s)	404, 651, 933, 975, 1113, 1198
BstDSI	CCRYGG	1 cut(s)	691
BstENI	CCTNNNNNAGG	1 cut(s)	1239
BstF5I	GGATG	5 cut(s)	172, 190, 501, 844, 1319
BstH2I	RGCGCY	1 cut(s)	115
BstHHI	GCGC	1 cut(s)	114
BstKTI	GATC	7 cut(s)	199, 279, 478, 484, 727, 1095, 1168
BstMAI	GTCTC	1 cut(s)	317
BstMBI	GATC	7 cut(s)	196, 276, 475, 481, 724, 1092, 1165
BstMWI	GCNNNNNNNGC	7 cut(s)	710, 977, 986, 992, 995, 1046, 1154
BstNI	CCWGG	2 cut(s)	458, 954
BstNSI	RCATGY	3 cut(s)	949, 1090, 1161
BstSCI	CCNGG	3 cut(s)	291, 456, 952
BstSFI	CTRYAG	2 cut(s)	987, 1038
BstSLI	GKGCMC	2 cut(s)	650, 973
BstV1I	GCAGC	8 cut(s)	308, 462, 973, 979, 1001, 1024, 1133, 1296
BstV2I	GAAGAC	2 cut(s)	129, 200
BstX2I	RGATCY	2 cut(s)	196, 481
BstXI	CCANNNNNNTGG	2 cut(s)	287, 1254
BstYI	RGATCY	2 cut(s)	196, 481
Bsu36I	CCTNAGG	2 cut(s)	975, 1198
BsuRI	GGCC	3 cut(s)	291, 1196, 1245
BtgI	CCRYGG	1 cut(s)	691
BtsCI	GGATG	5 cut(s)	172, 190, 501, 844, 1319
BtsIMutI	CAGTG	5 cut(s)	272, 401, 648, 1095, 1259
BveI	ACCTGC	3 cut(s)	704, 991, 1269
Cac8I	GCNNGC	4 cut(s)	158, 688, 1119, 1266
CfoI	GCGC	1 cut(s)	114
Cfr13I	GGNCC	6 cut(s)	11, 223, 1187, 1194, 1231, 1244
CseI	GACGC	1 cut(s)	504
Csp6I	GTAC	3 cut(s)	397, 564, 907
CviAII	CATG	7 cut(s)	532, 602, 636, 799, 946, 1087, 1158
CviQI	GTAC	3 cut(s)	397, 564, 907
DdeI	CTNAG	6 cut(s)	404, 651, 933, 975, 1113, 1198
DpnI	GATC	7 cut(s)	198, 278, 477, 483, 726, 1094, 1167
DpnII	GATC	7 cut(s)	196, 276, 475, 481, 724, 1092, 1165
DriI	GACNNNNNGTC	1 cut(s)	547
EaeI	YGGCCR	1 cut(s)	289
Eam1104I	CTCTTC	2 cut(s)	177, 795
Eam1105I	GACNNNNNGTC	1 cut(s)	547
EarI	CTCTTC	2 cut(s)	177, 795
EciI	GGCGGA	2 cut(s)	424, 1218
Ecl136II	GAGCTC	1 cut(s)	97
Eco130I	CCWWGG	2 cut(s)	219, 1190
Eco24I	GRGCYC	1 cut(s)	99
Eco32I	GATATC	1 cut(s)	298
Eco47I	GGWCC	4 cut(s)	11, 223, 1187, 1231
Eco53kI	GAGCTC	1 cut(s)	97
Eco72I	CACGTG	1 cut(s)	731
Eco81I	CCTNAGG	2 cut(s)	975, 1198
Eco88I	CYCGRG	1 cut(s)	325
EcoICRI	GAGCTC	1 cut(s)	97
EcoNI	CCTNNNNNAGG	1 cut(s)	1239
EcoO109I	RGGNCCY	3 cut(s)	11, 1194, 1231
EcoRII	CCWGG	2 cut(s)	456, 952
EcoRV	GATATC	1 cut(s)	298
EcoT14I	CCWWGG	2 cut(s)	219, 1190
EcoT22I	ATGCAT	1 cut(s)	501
EcoT38I	GRGCYC	1 cut(s)	99
ErhI	CCWWGG	2 cut(s)	219, 1190
FaeI	CATG	7 cut(s)	535, 605, 639, 802, 949, 1090, 1161
FaqI	GGGAC	4 cut(s)	583, 937, 1217, 1222
FatI	CATG	7 cut(s)	531, 601, 635, 798, 945, 1086, 1157
Fnu4HI	GCNGC	9 cut(s)	322, 451, 987, 990, 993, 996, 1038, 1122, 1285
FokI	GGATG	4 cut(s)	179, 197, 508, 851
FriOI	GRGCYC	1 cut(s)	99
Fsp4HI	GCNGC	9 cut(s)	322, 451, 987, 990, 993, 996, 1038, 1122, 1285
FspBI	CTAG	6 cut(s)	507, 666, 795, 810, 1191, 1269
GlaI	GCGC	1 cut(s)	113
GluI	GCNGC	9 cut(s)	322, 451, 987, 990, 993, 996, 1038, 1122, 1285
GsaI	CCCAGC	2 cut(s)	45, 969
HaeII	RGCGCY	1 cut(s)	115
HaeIII	GGCC	3 cut(s)	291, 1196, 1245
HapII	CCGG	1 cut(s)	292
HgaI	GACGC	1 cut(s)	504
HhaI	GCGC	1 cut(s)	114
Hin1I	GRCGYC	1 cut(s)	515
Hin1II	CATG	7 cut(s)	535, 605, 639, 802, 949, 1090, 1161
Hin6I	GCGC	1 cut(s)	112
HinP1I	GCGC	1 cut(s)	112
HinfI	GANTC	4 cut(s)	263, 379, 548, 787
HpaII	CCGG	1 cut(s)	292
HphI	GGTGA	2 cut(s)	74, 1318
Hpy166II	GTNNAC	5 cut(s)	272, 399, 439, 907, 1187
Hpy188I	TCNGA	9 cut(s)	166, 201, 262, 334, 378, 384, 488, 541, 769
Hpy188III	TCNNGA	2 cut(s)	479, 742
Hpy8I	GTNNAC	5 cut(s)	272, 399, 439, 907, 1187
Hpy99I	CGWCG	1 cut(s)	492
HpyAV	CCTTC	5 cut(s)	24, 142, 326, 913, 1287
HpyCH4III	ACNGT	4 cut(s)	86, 311, 1099, 1254
HpyCH4IV	ACGT	4 cut(s)	490, 596, 730, 893
HpyCH4V	TGCA	9 cut(s)	156, 450, 499, 686, 704, 989, 1040, 1049, 1121
HpyF10VI	GCNNNNNNNGC	7 cut(s)	710, 977, 986, 992, 995, 1046, 1154
HpyF3I	CTNAG	6 cut(s)	404, 651, 933, 975, 1113, 1198
HpySE526I	ACGT	4 cut(s)	490, 596, 730, 893
Hsp92I	GRCGYC	1 cut(s)	515
Hsp92II	CATG	7 cut(s)	535, 605, 639, 802, 949, 1090, 1161
HspAI	GCGC	1 cut(s)	112
KflI	GGGWCCC	1 cut(s)	1231
Kzo9I	GATC	7 cut(s)	196, 276, 475, 481, 724, 1092, 1165
LguI	GCTCTTC	1 cut(s)	177
LmnI	GCTCC	2 cut(s)	94, 391
Lsp1109I	GCAGC	8 cut(s)	308, 462, 973, 979, 1001, 1024, 1133, 1296
LweI	GCATC	5 cut(s)	157, 250, 486, 659, 1304
MaeI	CTAG	6 cut(s)	507, 666, 795, 810, 1191, 1269
MaeII	ACGT	4 cut(s)	490, 596, 730, 893
MalI	GATC	7 cut(s)	198, 278, 477, 483, 726, 1094, 1167
MbiI	CCGCTC	1 cut(s)	372
MboI	GATC	7 cut(s)	196, 276, 475, 481, 724, 1092, 1165
MboII	GAAGA	5 cut(s)	134, 164, 200, 782, 969
MfeI	CAATTG	1 cut(s)	1212
MflI	RGATCY	2 cut(s)	196, 481
MhlI	GDGCHC	4 cut(s)	99, 650, 824, 973
MluCI	AATT	9 cut(s)	256, 699, 705, 763, 1173, 1179, 1212, 1222, 1297
MlyI	GAGTC	2 cut(s)	557, 781
MmeI	TCCRAC	2 cut(s)	473, 511
Mph1103I	ATGCAT	1 cut(s)	501
MroXI	GAANNNNTTC	1 cut(s)	1276
MseI	TTAA	2 cut(s)	1172, 1221
MslI	CAYNNNNRTG	3 cut(s)	653, 667, 1252
MspA1I	CMGCKG	1 cut(s)	992
MspI	CCGG	1 cut(s)	292
MspR9I	CCNGG	3 cut(s)	293, 458, 954
MunI	CAATTG	1 cut(s)	1212
MvaI	CCWGG	2 cut(s)	458, 954
MwoI	GCNNNNNNNGC	7 cut(s)	710, 977, 986, 992, 995, 1046, 1154
NciI	CCSGG	1 cut(s)	293
NdeII	GATC	7 cut(s)	196, 276, 475, 481, 724, 1092, 1165
NlaIII	CATG	7 cut(s)	535, 605, 639, 802, 949, 1090, 1161
NlaIV	GGNNCC	6 cut(s)	972, 981, 999, 1232, 1233, 1259
NsiI	ATGCAT	1 cut(s)	501
NspI	RCATGY	3 cut(s)	949, 1090, 1161
OliI	CACNNNNGTG	1 cut(s)	1252
PaeR7I	CTCGAG	1 cut(s)	325
PaqCI	CACCTGC	2 cut(s)	704, 991
PciI	ACATGT	2 cut(s)	945, 1086
PciSI	GCTCTTC	1 cut(s)	177
PdmI	GAANNNNTTC	1 cut(s)	1276
PfeI	GAWTC	2 cut(s)	263, 379
PflMI	CCANNNNNTGG	1 cut(s)	636
PfoI	TCCNGGA	1 cut(s)	456
PkrI	GCNGC	9 cut(s)	323, 452, 988, 991, 994, 997, 1039, 1123, 1286
PleI	GAGTC	2 cut(s)	556, 781
PmaCI	CACGTG	1 cut(s)	731
PmlI	CACGTG	1 cut(s)	731
PpsI	GAGTC	2 cut(s)	556, 781
Ppu21I	YACGTR	2 cut(s)	731, 894
PpuMI	RGGWCCY	2 cut(s)	11, 1231
PscI	ACATGT	2 cut(s)	945, 1086
PsiI	TTATAA	1 cut(s)	762
Psp124BI	GAGCTC	1 cut(s)	99
Psp5II	RGGWCCY	2 cut(s)	11, 1231
Psp6I	CCWGG	2 cut(s)	456, 952
PspCI	CACGTG	1 cut(s)	731
PspFI	CCCAGC	2 cut(s)	41, 965
PspGI	CCWGG	2 cut(s)	456, 952
PspN4I	GGNNCC	6 cut(s)	972, 981, 999, 1232, 1233, 1259
PspPI	GGNCC	6 cut(s)	11, 223, 1187, 1194, 1231, 1244
PspPPI	RGGWCCY	2 cut(s)	11, 1231
PspXI	VCTCGAGB	1 cut(s)	325
PstI	CTGCAG	2 cut(s)	991, 1042
PsuI	RGATCY	2 cut(s)	196, 481
PvuII	CAGCTG	1 cut(s)	992
RsaI	GTAC	3 cut(s)	398, 565, 908
RsaNI	GTAC	3 cut(s)	397, 564, 907
RseI	CAYNNNNRTG	3 cut(s)	653, 667, 1252
SacI	GAGCTC	1 cut(s)	99
SapI	GCTCTTC	1 cut(s)	177
SaqAI	TTAA	2 cut(s)	1172, 1221
SatI	GCNGC	9 cut(s)	322, 451, 987, 990, 993, 996, 1038, 1122, 1285
Sau3AI	GATC	7 cut(s)	196, 276, 475, 481, 724, 1092, 1165
Sau96I	GGNCC	6 cut(s)	11, 223, 1187, 1194, 1231, 1244
SchI	GAGTC	2 cut(s)	557, 781
ScrFI	CCNGG	3 cut(s)	293, 458, 954
SduI	GDGCHC	4 cut(s)	99, 650, 824, 973
SfaNI	GCATC	5 cut(s)	157, 250, 486, 659, 1304
SfcI	CTRYAG	2 cut(s)	987, 1038
Sfr274I	CTCGAG	1 cut(s)	325
SinI	GGWCC	4 cut(s)	11, 223, 1187, 1231
SlaI	CTCGAG	1 cut(s)	325
SmiMI	CAYNNNNRTG	3 cut(s)	653, 667, 1252
SmlI	CTYRAG	1 cut(s)	325
SmoI	CTYRAG	1 cut(s)	325
Sse9I	AATT	9 cut(s)	256, 699, 705, 763, 1173, 1179, 1212, 1222, 1297
SsiI	CCGC	5 cut(s)	370, 409, 995, 1203, 1304
SspMI	CTAG	6 cut(s)	507, 666, 795, 810, 1191, 1269
SstI	GAGCTC	1 cut(s)	99
StyD4I	CCNGG	3 cut(s)	291, 456, 952
StyI	CCWWGG	2 cut(s)	219, 1190
TaaI	ACNGT	4 cut(s)	86, 311, 1099, 1254
TaiI	ACGT	4 cut(s)	493, 599, 733, 896
TaqI	TCGA	2 cut(s)	326, 1127
TasI	AATT	9 cut(s)	256, 699, 705, 763, 1173, 1179, 1212, 1222, 1297
TatI	WGTACW	1 cut(s)	396
TauI	GCSGC	1 cut(s)	998
TfiI	GAWTC	2 cut(s)	263, 379
Tru1I	TTAA	2 cut(s)	1172, 1221
Tru9I	TTAA	2 cut(s)	1172, 1221
TscAI	CASTG	5 cut(s)	279, 408, 655, 1102, 1259
TseI	GCWGC	8 cut(s)	321, 450, 986, 989, 992, 1037, 1121, 1284
TspDTI	ATGAA	5 cut(s)	147, 520, 671, 815, 1145
TspGWI	ACGGA	1 cut(s)	581
TspRI	CASTG	5 cut(s)	279, 408, 655, 1102, 1259
Van91I	CCANNNNNTGG	1 cut(s)	636
VpaK11BI	GGWCC	4 cut(s)	11, 223, 1187, 1231
XagI	CCTNNNNNAGG	1 cut(s)	1239
XceI	RCATGY	3 cut(s)	949, 1090, 1161
XhoI	CTCGAG	1 cut(s)	325
XmaJI	CCTAGG	1 cut(s)	1190
XmnI	GAANNNNTTC	1 cut(s)	1276
XspI	CTAG	6 cut(s)	507, 666, 795, 810, 1191, 1269
Zsp2I	ATGCAT	1 cut(s)	501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.