MD14G1203500.v1.1

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005502
Physical Location & Seq
Forward (+)
29185596 .. 29187613
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1203500.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGAATTGGGTTGTGACTCTCGAGGCTATTCTCGTGCTGGATATGATACCGTTACTTGGTACTGTGCTGTTGTAGCAGGGAGGCATAGCCCAGAACTCAGTAATATGCGTGGAACCATTTGCAGGGTTGAAGAGAAGTTGTCAAATGAAGTCGAGACAAGGTCAGCTGATGAAGAGCAATCTATAGCACCAGCAGCAGGTGCGAGTAAAAGCAAACGACTGACATTTGCTTGGCTTGACGGAGAAGCCCAGAAGAAATATTGCCTCTTCTTCCTTCAATCTGAAAACATCCATGAAACTTGTGGTGAAAGGACAGATATGATTGATGTTCCTCAGTTATTTATTGTACGCTATAAGAGGAATGTGACAGAAGAGAGTGAGGAGCCCGAGAAAAAGCCGAAAACCATATGGGATGGACTGCAAGACCAGGAACTAGATCCTGCATCACGGCTTGTGGCAAAGTACAATGGTTCAGATAATACTTGGAAGATTATTAAATGGATCTCAGAAACAATCTACGATGGTGACGCCAGAGATCTTCCTCATTATAGAACAAGAACTCCTCATTTGGTTCCCGAGGATTCAGAGCCAATGTGGTTAGCAGGTGTCCAAAGAATTCCTTCAACAAACACGATCATGCAGAGCATCCGTGGAATTTTACACGGAATCTATGATTGCATTGGAGATCCAAGGGTTGGTCAAATGTTGCTTCTAGCAGCATTGATGTCTTTCCGTACCATCTGGCTTCAGAGGAGTCAAGCAACTCCAGTGTCAAGTCAACCAAATCAGCCAAATTCCACGGTGAACATTAAGTGCAAGAAAGACGAAAGTAGACGAAGGAGAGACCGGGCTAGAAACGTGCTTAATGAAGACCTACCCCCTTCCATCACCGATATGGAACCAAGAGATTCTTATGAAATGCTACTGTCAGGTTCCGATTCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001671 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0006457 GO:0006508 GO:0006511 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0008047 GO:0008150 GO:0008152 GO:0008219 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0010563 GO:0010605 GO:0012501 GO:0012505 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0016671 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030234 GO:0030433 GO:0030544 GO:0031072 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031974 GO:0032268 GO:0032269 GO:0032781 GO:0032991 GO:0033554 GO:0034663 GO:0034975 GO:0034976 GO:0035556 GO:0036503 GO:0042221 GO:0042325 GO:0042326 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043462 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045936 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051087 GO:0051117 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051336 GO:0051345 GO:0051603 GO:0051716 GO:0051787 GO:0055114 GO:0060255 GO:0060589 GO:0060590 GO:0065007 GO:0065009 GO:0070013 GO:0070059 GO:0071704 GO:0080090 GO:0097190 GO:0097193 GO:0098772 GO:1901564 GO:1901565 GO:1901575 GO:1901698
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

316

Amino Acids

35.89

Weight (kDa)

5.47

Isoelectric Point (pI)

61.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 188, 593
Acc36I ACCTGC 2 cut(s) 188, 593
AccB7I CCANNNNNTGG 1 cut(s) 695
AccI GTMKAC 1 cut(s) 832
AclWI GGATC 3 cut(s) 431, 509, 680
AcsI RAATTY 3 cut(s) 615, 654, 793
AcuI CTGAAG 1 cut(s) 731
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 4 cut(s) 62, 348, 464, 736
AfiI CCNNNNNNNGG 4 cut(s) 57, 123, 197, 695
AgsI TTSAA 3 cut(s) 131, 278, 624
AjnI CCWGG 1 cut(s) 426
AloI GAACNNNNNNTCC 2 cut(s) 544, 576
AluBI AGCT 1 cut(s) 167
AluI AGCT 1 cut(s) 167
Alw26I GTCTC 2 cut(s) 149, 837
AlwI GGATC 3 cut(s) 431, 509, 680
Ama87I CYCGRG 3 cut(s) 21, 386, 575
ApeKI GCWGC 2 cut(s) 194, 716
ApoI RAATTY 3 cut(s) 615, 654, 793
Asp700I GAANNNNTTC 1 cut(s) 619
AsuC2I CCSGG 1 cut(s) 848
AsuHPI GGTGA 4 cut(s) 317, 536, 814, 880
AvaI CYCGRG 3 cut(s) 21, 386, 575
BanII GRGCYC 1 cut(s) 387
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 876
BbvI GCAGC 2 cut(s) 206, 728
BccI CCATC 4 cut(s) 407, 515, 746, 893
BceAI ACGGC 1 cut(s) 464
BciT130I CCWGG 1 cut(s) 428
BcnI CCSGG 1 cut(s) 848
BcoDI GTCTC 2 cut(s) 149, 837
BfaI CTAG 3 cut(s) 434, 713, 852
BfmI CTRYAG 1 cut(s) 183
BfuAI ACCTGC 2 cut(s) 188, 593
BglII AGATCT 1 cut(s) 535
BisI GCNGC 2 cut(s) 195, 717
BlsI GCNGC 2 cut(s) 196, 718
Bme1390I CCNGG 2 cut(s) 428, 848
BmeT110I CYCGRG 3 cut(s) 21, 386, 575
BmiI GGNNCC 5 cut(s) 115, 384, 573, 900, 934
BmrFI CCNGG 2 cut(s) 428, 848
BmsI GCATC 2 cut(s) 452, 654
BpiI GAAGAC 1 cut(s) 876
BpmI CTGGAG 1 cut(s) 750
BpuMI CCSGG 1 cut(s) 848
BsaHI GRCGYC 1 cut(s) 528
BsaI GGTCTC 1 cut(s) 837
BsaJI CCNNGG 4 cut(s) 576, 649, 689, 798
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 123, 197, 695
Bse1I ACTGG 1 cut(s) 767
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 4 cut(s) 576, 649, 689, 798
BseGI GGATG 3 cut(s) 288, 418, 645
BseLI CCNNNNNNNGG 4 cut(s) 57, 123, 197, 695
BseMII CTCAG 3 cut(s) 112, 347, 519
BseNI ACTGG 1 cut(s) 767
BseRI GAGGAG 3 cut(s) 395, 552, 766
BseXI GCAGC 2 cut(s) 206, 728
BsiHKCI CYCGRG 3 cut(s) 21, 386, 575
BsiSI CCGG 1 cut(s) 847
BslI CCNNNNNNNGG 4 cut(s) 57, 123, 197, 695
BsmAI GTCTC 2 cut(s) 149, 837
Bso31I GGTCTC 1 cut(s) 837
BsoBI CYCGRG 3 cut(s) 21, 386, 575
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 5 cut(s) 436, 501, 535, 633, 685
BspCNI CTCAG 3 cut(s) 111, 346, 518
BspLI GGNNCC 5 cut(s) 115, 384, 573, 900, 934
BspMI ACCTGC 2 cut(s) 188, 593
BspPI GGATC 3 cut(s) 431, 509, 680
BspQI GCTCTTC 1 cut(s) 168
BspTNI GGTCTC 1 cut(s) 837
BsrI ACTGG 1 cut(s) 767
BssECI CCNNGG 4 cut(s) 576, 649, 689, 798
BssMI GATC 5 cut(s) 436, 501, 535, 633, 685
BssNI GRCGYC 1 cut(s) 528
BssSI CACGAG 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 689
Bst2BI CACGAG 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 4 cut(s) 52, 65, 802, 927
Bst6I CTCTTC 4 cut(s) 126, 168, 272, 366
BstACI GRCGYC 1 cut(s) 528
BstAPI GCANNNNNTGC 1 cut(s) 200
BstDEI CTNAG 3 cut(s) 98, 333, 505
BstDSI CCRYGG 2 cut(s) 649, 798
BstF5I GGATG 3 cut(s) 288, 418, 645
BstKTI GATC 5 cut(s) 439, 504, 538, 636, 688
BstMAI GTCTC 2 cut(s) 149, 837
BstMBI GATC 5 cut(s) 436, 501, 535, 633, 685
BstMWI GCNNNNNNNGC 3 cut(s) 74, 194, 200
BstNI CCWGG 1 cut(s) 428
BstSCI CCNGG 2 cut(s) 426, 846
BstSFI CTRYAG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 206, 728
BstV2I GAAGAC 1 cut(s) 876
BstX2I RGATCY 4 cut(s) 436, 501, 535, 685
BstYI RGATCY 4 cut(s) 436, 501, 535, 685
BtgI CCRYGG 2 cut(s) 649, 798
BtsCI GGATG 3 cut(s) 288, 418, 645
BtsIMutI CAGTG 1 cut(s) 774
BveI ACCTGC 2 cut(s) 188, 593
CseI GACGC 1 cut(s) 536
Csp6I GTAC 4 cut(s) 61, 347, 463, 735
CviAII CATG 2 cut(s) 293, 637
CviQI GTAC 4 cut(s) 61, 347, 463, 735
DdeI CTNAG 3 cut(s) 98, 333, 505
DpnI GATC 5 cut(s) 438, 503, 537, 635, 687
DpnII GATC 5 cut(s) 436, 501, 535, 633, 685
Eam1104I CTCTTC 4 cut(s) 126, 168, 272, 366
EarI CTCTTC 4 cut(s) 126, 168, 272, 366
Eco130I CCWWGG 1 cut(s) 689
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 837
Eco57I CTGAAG 1 cut(s) 731
Eco88I CYCGRG 3 cut(s) 21, 386, 575
EcoRI GAATTC 1 cut(s) 615
EcoRII CCWGG 1 cut(s) 426
EcoT14I CCWWGG 1 cut(s) 689
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 689
FaeI CATG 2 cut(s) 296, 640
FalI AAGNNNNNCTT 4 cut(s) 604, 636, 895, 927
FatI CATG 2 cut(s) 292, 636
FauNDI CATATG 1 cut(s) 407
FblI GTMKAC 1 cut(s) 832
Fnu4HI GCNGC 2 cut(s) 195, 717
FokI GGATG 3 cut(s) 275, 425, 632
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 2 cut(s) 195, 717
FspBI CTAG 3 cut(s) 434, 713, 852
GluI GCNGC 2 cut(s) 195, 717
GsuI CTGGAG 1 cut(s) 750
HapII CCGG 1 cut(s) 847
HgaI GACGC 1 cut(s) 536
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 2 cut(s) 296, 640
HincII GTYRAC 1 cut(s) 779
HindII GTYRAC 1 cut(s) 779
HinfI GANTC 6 cut(s) 17, 581, 666, 754, 908, 938
HpaII CCGG 1 cut(s) 847
HphI GGTGA 4 cut(s) 317, 536, 814, 880
Hpy166II GTNNAC 3 cut(s) 779, 805, 833
Hpy188I TCNGA 7 cut(s) 283, 475, 508, 586, 750, 937, 943
Hpy188III TCNNGA 3 cut(s) 21, 154, 575
Hpy8I GTNNAC 3 cut(s) 779, 805, 833
HpyAV CCTTC 4 cut(s) 284, 630, 831, 891
HpyCH4III ACNGT 4 cut(s) 52, 65, 802, 927
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 6 cut(s) 123, 421, 443, 640, 678, 816
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 194, 200
HpyF3I CTNAG 3 cut(s) 98, 333, 505
HpySE526I ACGT 1 cut(s) 858
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 2 cut(s) 296, 640
Kzo9I GATC 5 cut(s) 436, 501, 535, 633, 685
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 1 cut(s) 382
Lsp1109I GCAGC 2 cut(s) 206, 728
LweI GCATC 2 cut(s) 452, 654
MaeI CTAG 3 cut(s) 434, 713, 852
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 4 cut(s) 14, 52, 364, 524
MalI GATC 5 cut(s) 438, 503, 537, 635, 687
MboI GATC 5 cut(s) 436, 501, 535, 633, 685
MboII GAAGA 9 cut(s) 143, 185, 259, 262, 265, 383, 499, 530, 881
MflI RGATCY 4 cut(s) 436, 501, 535, 685
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 4 cut(s) 5, 615, 654, 793
MlyI GAGTC 2 cut(s) 11, 763
MroXI GAANNNNTTC 1 cut(s) 619
MseI TTAA 3 cut(s) 495, 810, 864
MslI CAYNNNNRTG 2 cut(s) 635, 893
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 1 cut(s) 847
MspR9I CCNGG 2 cut(s) 428, 848
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 3 cut(s) 74, 194, 200
NciI CCSGG 1 cut(s) 848
NdeI CATATG 1 cut(s) 407
NdeII GATC 5 cut(s) 436, 501, 535, 633, 685
NlaIII CATG 2 cut(s) 296, 640
NlaIV GGNNCC 5 cut(s) 115, 384, 573, 900, 934
NmuCI GTSAC 3 cut(s) 14, 364, 524
PaeR7I CTCGAG 1 cut(s) 21
PaqCI CACCTGC 2 cut(s) 188, 593
PciSI GCTCTTC 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 619
PfeI GAWTC 4 cut(s) 581, 666, 908, 938
PflFI GACNNNGTC 1 cut(s) 160
PflMI CCANNNNNTGG 1 cut(s) 695
PkrI GCNGC 2 cut(s) 196, 718
PleI GAGTC 2 cut(s) 11, 762
PpsI GAGTC 2 cut(s) 11, 762
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 5 cut(s) 115, 384, 573, 900, 934
PsuI RGATCY 4 cut(s) 436, 501, 535, 685
PsyI GACNNNGTC 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 4 cut(s) 62, 348, 464, 736
RsaNI GTAC 4 cut(s) 61, 347, 463, 735
RseI CAYNNNNRTG 2 cut(s) 635, 893
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 3 cut(s) 495, 810, 864
SatI GCNGC 2 cut(s) 195, 717
Sau3AI GATC 5 cut(s) 436, 501, 535, 633, 685
SchI GAGTC 2 cut(s) 11, 763
ScrFI CCNGG 2 cut(s) 428, 848
SduI GDGCHC 1 cut(s) 387
SetI ASST 7 cut(s) 164, 169, 202, 607, 861, 876, 934
SfaNI GCATC 2 cut(s) 452, 654
SfcI CTRYAG 1 cut(s) 183
Sfr274I CTCGAG 1 cut(s) 21
SlaI CTCGAG 1 cut(s) 21
SmiMI CAYNNNNRTG 2 cut(s) 635, 893
SmlI CTYRAG 1 cut(s) 21
SmoI CTYRAG 1 cut(s) 21
Sse9I AATT 4 cut(s) 5, 615, 654, 793
SspI AATATT 1 cut(s) 260
SspMI CTAG 3 cut(s) 434, 713, 852
StyD4I CCNGG 2 cut(s) 426, 846
StyI CCWWGG 1 cut(s) 689
TaaI ACNGT 4 cut(s) 52, 65, 802, 927
TaiI ACGT 1 cut(s) 861
TaqI TCGA 2 cut(s) 22, 153
TasI AATT 4 cut(s) 5, 615, 654, 793
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 4 cut(s) 581, 666, 908, 938
Tru1I TTAA 3 cut(s) 495, 810, 864
Tru9I TTAA 3 cut(s) 495, 810, 864
TscAI CASTG 1 cut(s) 774
TseFI GTSAC 3 cut(s) 14, 364, 524
TseI GCWGC 2 cut(s) 194, 716
Tsp45I GTSAC 3 cut(s) 14, 364, 524
TspDTI ATGAA 5 cut(s) 162, 186, 309, 882, 930
TspGWI ACGGA 4 cut(s) 255, 638, 678, 722
TspRI CASTG 1 cut(s) 774
Tth111I GACNNNGTC 1 cut(s) 160
Van91I CCANNNNNTGG 1 cut(s) 695
XapI RAATTY 3 cut(s) 615, 654, 793
XcmI CCANNNNNNNNNTGG 2 cut(s) 299, 892
XhoI CTCGAG 1 cut(s) 21
XmiI GTMKAC 1 cut(s) 832
XmnI GAANNNNTTC 1 cut(s) 619
XspI CTAG 3 cut(s) 434, 713, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.