Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005439

Physical Location & Seq

Genomic Coordinates Reverse (-)

29684974 .. 29688022

Gene Structure 3049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1211600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 762 bp

ATGAGACAACATCCCAAATTACGGAACTCGATTGCTGCTTCAGCTCAACCATTAGAAGCCACATTGAGCCCCTTTGACAACAGGCTACCATCCAAAGAGGTTCTTGATTTATGGAGAAATGCTGATGCGGTATGCTTCGATGTGGATAGCACGGTTTGCCTGGATGAGGGCATTGATGAACTTGCAGAACATTGTGGAGCTGGAAAGGCTGTTGCAGAATGGACTGCTAGGGCAATGGGTGGTTCAGTACCTTTTGAGGAGGCTTTGGCTGCTAGACTATCTTTGTTCAATCCTTCCCTGTCTCAAGTCCAAGATTTTCTCGAAAAGAACCCCCCAAAGCTATCTCCTGGCATAGTCGAGTTAGTCAAGAAGCTGAAGGCTAATGGCACCGACGTTTACCTGGTCTCTGGAGGCTTCAGTCAAATGATCAAACCTGTGGCGGCGATTCTTGATATACCAACTGAAAACGTGTTTGCTAATCAACTACTGTTTGGAACTTTTGGAGAGTTTCTGGGTTTTGACAAAAACGAGCCCACTTCAAGGAGTGGCGGAAAAGCCACCGCAGTGCAACAGATAAGGAAGGCTCACAATTACAAGGAACTTGTCATGATTGGGGATGGCGCGACTGATCTTGAGGCACGCCAACCCGGAGGTGCTGACTTGTTCATCTGCTACGCTGGTGTTCAACTACGCGAGGCTGTTGCTGCAAAGGCTGATTGGCTTGTTCTTAATCTTCAAGACATGATAAGTTCCTTGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004647 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006563 GO:0006564 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009507 GO:0009536 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019752 GO:0022414 GO:0022622 GO:0032501 GO:0032502 GO:0042578 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046872 GO:0048229 GO:0048316 GO:0048364 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

HAD Hydrolase

Protein Analysis

254

Amino Acids

27.33

Weight (kDa)

4.93

Isoelectric Point (pI)

33.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	42 - 213	9.1e-17	haloacid dehalogenase-like hydrolase
HAD	PF12710	45 - 212	2.7e-12	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005439)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18640 AT1G18640
fragaria_vesca	FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840
malus_domestica	MD06G1202500.v1.1 MD14G1211600.v1.1
prunus_persica	Prupe.5G205100_v2.0.a1
pyrus_communis	pycom06g17850 pycom14g17520
rosa_chinensis	RchiOBHm_Chr7g0183091
rosa_laevigata	RLG00000005079
rosa_multiflora	Rmu_sc0012218.1_g000001 Rmu_ssc0000111.1_g000006
rosa_roxburghii	Rroxscaffold_3G00270770
rosa_rugosa	Rorug06G0452700 Rorug06G0452800 Rorug06G0452800 Rorug06G0452900
rosa_samantha	Rh7AG056000 Rh7BG055300 Rh7CG056800 Rh7DG055500
rosa_wichuraiana	Rw7G004560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	386
AccII	CGCG	2 cut(s)	623, 693
AciI	CCGC	4 cut(s)	128, 440, 549, 561
AcuI	CTGAAG	3 cut(s)	24, 395, 400
AfaI	GTAC	1 cut(s)	249
AfiI	CCNNNNNNNGG	3 cut(s)	21, 166, 540
AflIII	ACRYGT	1 cut(s)	468
AgsI	TTSAA	4 cut(s)	289, 540, 686, 737
AjnI	CCWGG	3 cut(s)	159, 346, 399
AleI	CACNNNNGTG	1 cut(s)	563
AluBI	AGCT	4 cut(s)	44, 200, 340, 373
AluI	AGCT	4 cut(s)	44, 200, 340, 373
Alw26I	GTCTC	2 cut(s)	306, 409
ApeKI	GCWGC	3 cut(s)	35, 269, 704
AspLEI	GCGC	1 cut(s)	623
AsuC2I	CCSGG	1 cut(s)	648
BanI	GGYRCC	1 cut(s)	386
BanII	GRGCYC	2 cut(s)	71, 534
BbvI	GCAGC	3 cut(s)	22, 256, 691
BccI	CCATC	2 cut(s)	97, 611
BcgI	CGANNNNNNTGC	2 cut(s)	683, 717
BciT130I	CCWGG	3 cut(s)	161, 348, 401
BclI	TGATCA	1 cut(s)	426
BcnI	CCSGG	1 cut(s)	648
BcoDI	GTCTC	2 cut(s)	306, 409
BfaI	CTAG	2 cut(s)	228, 273
BisI	GCNGC	4 cut(s)	36, 270, 441, 705
BlsI	GCNGC	4 cut(s)	37, 271, 442, 706
Bme1390I	CCNGG	4 cut(s)	161, 348, 401, 648
BmiI	GGNNCC	1 cut(s)	388
BmrFI	CCNGG	4 cut(s)	161, 348, 401, 648
BmsI	GCATC	1 cut(s)	115
BpmI	CTGGAG	1 cut(s)	429
BpuEI	CTTGAG	2 cut(s)	288, 653
BpuMI	CCSGG	1 cut(s)	648
BsaI	GGTCTC	1 cut(s)	409
BsaJI	CCNNGG	1 cut(s)	753
Bsc4I	CCNNNNNNNGG	3 cut(s)	21, 166, 540
Bse3DI	GCAATG	1 cut(s)	240
BseBI	CCWGG	3 cut(s)	161, 348, 401
BseDI	CCNNGG	1 cut(s)	753
BseGI	GGATG	4 cut(s)	10, 89, 169, 622
BseLI	CCNNNNNNNGG	3 cut(s)	21, 166, 540
BseMI	GCAATG	1 cut(s)	240
BseRI	GAGGAG	1 cut(s)	272
BseXI	GCAGC	3 cut(s)	22, 256, 691
Bsh1236I	CGCG	2 cut(s)	623, 693
BshNI	GGYRCC	1 cut(s)	386
BsiSI	CCGG	1 cut(s)	648
BslI	CCNNNNNNNGG	3 cut(s)	21, 166, 540
BsmAI	GTCTC	2 cut(s)	306, 409
Bso31I	GGTCTC	1 cut(s)	409
Bsp1286I	GDGCHC	2 cut(s)	71, 534
Bsp143I	GATC	2 cut(s)	426, 628
BspACI	CCGC	4 cut(s)	128, 440, 549, 561
BspFNI	CGCG	2 cut(s)	623, 693
BspHI	TCATGA	1 cut(s)	606
BspLI	GGNNCC	1 cut(s)	388
BspT107I	GGYRCC	1 cut(s)	386
BspTNI	GGTCTC	1 cut(s)	409
BsrDI	GCAATG	1 cut(s)	240
BssECI	CCNNGG	1 cut(s)	753
BssMI	GATC	2 cut(s)	426, 628
BssT1I	CCWWGG	1 cut(s)	753
Bst2UI	CCWGG	3 cut(s)	161, 348, 401
Bst4CI	ACNGT	2 cut(s)	154, 489
BstAPI	GCANNNNNTGC	1 cut(s)	156
BstC8I	GCNNGC	1 cut(s)	640
BstENI	CCTNNNNNAGG	1 cut(s)	164
BstF5I	GGATG	4 cut(s)	10, 89, 169, 622
BstFNI	CGCG	2 cut(s)	623, 693
BstHHI	GCGC	1 cut(s)	623
BstKTI	GATC	2 cut(s)	429, 631
BstMAI	GTCTC	2 cut(s)	306, 409
BstMBI	GATC	2 cut(s)	426, 628
BstMWI	GCNNNNNNNGC	6 cut(s)	41, 156, 206, 269, 704, 710
BstNI	CCWGG	3 cut(s)	161, 348, 401
BstSCI	CCNGG	4 cut(s)	159, 346, 399, 646
BstUI	CGCG	2 cut(s)	623, 693
BstV1I	GCAGC	3 cut(s)	22, 256, 691
BtsCI	GGATG	4 cut(s)	10, 89, 169, 622
BtsI	GCAGTG	1 cut(s)	570
BtsIMutI	CAGTG	1 cut(s)	570
Cac8I	GCNNGC	1 cut(s)	640
CciI	TCATGA	1 cut(s)	606
CfoI	GCGC	1 cut(s)	623
CsiI	ACCWGGT	1 cut(s)	399
Csp6I	GTAC	1 cut(s)	248
CviAII	CATG	2 cut(s)	607, 742
CviQI	GTAC	1 cut(s)	248
DpnI	GATC	2 cut(s)	428, 630
DpnII	GATC	2 cut(s)	426, 628
EciI	GGCGGA	1 cut(s)	564
Eco130I	CCWWGG	1 cut(s)	753
Eco24I	GRGCYC	2 cut(s)	71, 534
Eco31I	GGTCTC	1 cut(s)	409
Eco57I	CTGAAG	3 cut(s)	24, 395, 400
EcoNI	CCTNNNNNAGG	1 cut(s)	164
EcoRII	CCWGG	3 cut(s)	159, 346, 399
EcoT14I	CCWWGG	1 cut(s)	753
EcoT38I	GRGCYC	2 cut(s)	71, 534
ErhI	CCWWGG	1 cut(s)	753
FaeI	CATG	2 cut(s)	610, 745
FaiI	YATR	6 cut(s)	112, 133, 353, 455, 608, 743
FalI	AAGNNNNNCTT	2 cut(s)	87, 119
FatI	CATG	2 cut(s)	606, 741
FbaI	TGATCA	1 cut(s)	426
Fnu4HI	GCNGC	4 cut(s)	36, 270, 441, 705
FokI	GGATG	3 cut(s)	76, 176, 629
FriOI	GRGCYC	2 cut(s)	71, 534
Fsp4HI	GCNGC	4 cut(s)	36, 270, 441, 705
FspBI	CTAG	2 cut(s)	228, 273
GlaI	GCGC	1 cut(s)	622
GluI	GCNGC	4 cut(s)	36, 270, 441, 705
GsuI	CTGGAG	1 cut(s)	429
HapII	CCGG	1 cut(s)	648
HhaI	GCGC	1 cut(s)	623
Hin1II	CATG	2 cut(s)	610, 745
Hin6I	GCGC	1 cut(s)	621
HinP1I	GCGC	1 cut(s)	621
HinfI	GANTC	1 cut(s)	445
HpaII	CCGG	1 cut(s)	648
Hpy166II	GTNNAC	1 cut(s)	397
Hpy188III	TCNNGA	8 cut(s)	104, 320, 367, 408, 449, 607, 632, 737
Hpy8I	GTNNAC	1 cut(s)	397
Hpy99I	CGWCG	1 cut(s)	395
HpyAV	CCTTC	3 cut(s)	303, 370, 574
HpyCH4III	ACNGT	2 cut(s)	154, 489
HpyCH4IV	ACGT	2 cut(s)	393, 468
HpyCH4V	TGCA	4 cut(s)	185, 215, 568, 707
HpyF10VI	GCNNNNNNNGC	6 cut(s)	41, 156, 206, 269, 704, 710
HpySE526I	ACGT	2 cut(s)	393, 468
Hsp92II	CATG	2 cut(s)	610, 745
HspAI	GCGC	1 cut(s)	621
Ksp22I	TGATCA	1 cut(s)	426
Kzo9I	GATC	2 cut(s)	426, 628
LmnI	GCTCC	1 cut(s)	197
Lsp1109I	GCAGC	3 cut(s)	22, 256, 691
LweI	GCATC	1 cut(s)	115
MabI	ACCWGGT	1 cut(s)	399
MaeI	CTAG	2 cut(s)	228, 273
MaeII	ACGT	2 cut(s)	393, 468
MalI	GATC	2 cut(s)	428, 630
MboI	GATC	2 cut(s)	426, 628
MboII	GAAGA	1 cut(s)	725
MhlI	GDGCHC	2 cut(s)	71, 534
MluCI	AATT	2 cut(s)	17, 589
MnlI	CCTC	8 cut(s)	91, 160, 250, 253, 404, 628, 644, 688
MseI	TTAA	2 cut(s)	729, 760
MslI	CAYNNNNRTG	1 cut(s)	563
MspI	CCGG	1 cut(s)	648
MspR9I	CCNGG	4 cut(s)	161, 348, 401, 648
MvaI	CCWGG	3 cut(s)	161, 348, 401
MvnI	CGCG	2 cut(s)	623, 693
MwoI	GCNNNNNNNGC	6 cut(s)	41, 156, 206, 269, 704, 710
NciI	CCSGG	1 cut(s)	648
NdeII	GATC	2 cut(s)	426, 628
NlaIII	CATG	2 cut(s)	610, 745
NlaIV	GGNNCC	1 cut(s)	388
OliI	CACNNNNGTG	1 cut(s)	563
PagI	TCATGA	1 cut(s)	606
PfeI	GAWTC	1 cut(s)	445
PkrI	GCNGC	4 cut(s)	37, 271, 442, 706
Psp6I	CCWGG	3 cut(s)	159, 346, 399
PspGI	CCWGG	3 cut(s)	159, 346, 399
PspN4I	GGNNCC	1 cut(s)	388
RsaI	GTAC	1 cut(s)	249
RsaNI	GTAC	1 cut(s)	248
RseI	CAYNNNNRTG	1 cut(s)	563
SaqAI	TTAA	2 cut(s)	729, 760
SatI	GCNGC	4 cut(s)	36, 270, 441, 705
Sau3AI	GATC	2 cut(s)	426, 628
ScrFI	CCNGG	4 cut(s)	161, 348, 401, 648
SduI	GDGCHC	2 cut(s)	71, 534
SexAI	ACCWGGT	1 cut(s)	399
SfaNI	GCATC	1 cut(s)	115
SmiMI	CAYNNNNRTG	1 cut(s)	563
SmlI	CTYRAG	2 cut(s)	303, 632
SmoI	CTYRAG	2 cut(s)	303, 632
Sse9I	AATT	2 cut(s)	17, 589
SsiI	CCGC	4 cut(s)	128, 440, 549, 561
SspMI	CTAG	2 cut(s)	228, 273
StyD4I	CCNGG	4 cut(s)	159, 346, 399, 646
StyI	CCWWGG	1 cut(s)	753
TaaI	ACNGT	2 cut(s)	154, 489
TaiI	ACGT	2 cut(s)	396, 471
TaqI	TCGA	4 cut(s)	29, 138, 321, 357
TasI	AATT	2 cut(s)	17, 589
TauI	GCSGC	1 cut(s)	443
TfiI	GAWTC	1 cut(s)	445
Tru1I	TTAA	2 cut(s)	729, 760
Tru9I	TTAA	2 cut(s)	729, 760
TscAI	CASTG	1 cut(s)	570
TseI	GCWGC	3 cut(s)	35, 269, 704
TspDTI	ATGAA	2 cut(s)	192, 655
TspGWI	ACGGA	1 cut(s)	37
TspRI	CASTG	1 cut(s)	570
XagI	CCTNNNNNAGG	1 cut(s)	164
XspI	CTAG	2 cut(s)	228, 273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.