Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0005439

Physical Location & Seq

Genomic Coordinates Forward (+)

58167461 .. 58181691

Gene Structure 14231 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0452800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6546 bp

ATGGCGTCTGGTTCGCCTCCTCCGTCGCGCATCAATCAGTTTTTTGCTTCGAAGAAACGGAAGCCTGTATCACCTGCTATGAAGTCGGGGAGAAATGAAAAGGATGTAAAAACCGTAGAAGGGTCACCTAGTGCTAAGGCCACATTGGACAGTTATTTGCGGACATCGCCGGAGAGTTATAATGTGGCGAAACCTTTGTGCAAATTTGGTGATTCAGCGGCTCGGCAAGATCAAGTTAGAAGGAATTTGGCATCCGAGATGGATAATTCTTCGAAAAATGAATGTATGCAGCACCGTTTCTCTTCTGAACTGCACACTTATGCAAGTGAGGCTTCTGGAGCAAATCAGAAGGAAATAGCTAAGGGATTAACCGAGGAGGGTGATGTTGCAGCTGGAGGTTCTTCCAAGGATTACTCAGATTTTGCAGGAGGTGTGGAGAATGTAGAGCTGAAGAAGTTTGCAGCCGACTTTTTGTCTTTGTATTGCAGTGATGTTCAATCAAACAAAAGTTCTATGTCAGAGCTGAAAGTGAATGGTCATAAGAGACACCCTAGCCCATCTTTGCTTGACGAGGAGAGTAAGACATTTAGAAAGAGGCATTGCGGTAGTGATAAATCCTATGAAGAGCGTGAAACAATTCGTTCTACTGAGAAGAGCTATGAAGCAATGCAATCTGCTTGTGTTGATAAAAATAGGGTTACCATTCCTAATGAATTGCTCGAGCTTCAACAAACCTTGAAAACATGCAGTAATACACCCAAACTGTCTCTAGATGTAGTTGATTGTTATACACCAGGCTCATTGATTAAATCATGTGTTCGTCAGACCCCCAATTCAATACGTGGTAGCTCATTTTCTTCACCTGGTGAAGCTTTTTGGAACGATGCAATTCAACTTGCTGATGGTCTATGTGCCCAGGCAGCAGAAGCAACTACAGCTTCAGATAGCCAATATCATGGTATAAACTCTTGCAATTTGAGAAATGCACACTGTGACGAAAAGTCAAAGGAAATGATGGATGAAGGTGAGCGTATGGGTAATGGTGCTGATTCAGAGCCAATGGTAAAGCATAGGAAGGACTTGCATAAAGAAGTATCCCCACTGCCTGTTAAGCATTTTGATTTCTCTCGTGAGGACAAGATTTTGAATGAAAGTATGCCACATCATATAGATTCTTACAATTTGAAAAGCACGGCTCATGGAGGTGGCGAGCAATCTGAATCCAGTCTTATAGATGCTAGAGGTCTAAGACATGGTATTACTATGGTCCGTTGCAGCAAATCTCAGGCAAATCAAGGGCAAGATGTTGATTCTGTCACTGCAGTGACCAAAACGAAAGTGGATTTGTCTAGCAAGGATAATGGTGGCCCGACATTTTCTTCACCAGGTGATGATGTCGTTAAGTTGAATGGTAAAGGTGAATCTAGCGAAGCGAGTACTCCTTCAAGTTTTCTGCCACTGCAAGATTGTCTGGGTCTAGATGGTTGGCTTGCACCAGAAATATGCAGCATATATAGAAAGAAAGGAATTTCCAAGCTTTATCCTTGGCAGGTTGAGTGCCTTCAGGTTGATGGTGTATTGCAGAGAAGGAATCTTGTGTATTGTGCATCAACGAGTGCAGGTAAAAGTTTTGTTGCAGAAATTCTGATGCTGCGGCAGGTCTTGTCATCTGGAAAAATAGCTTTACTTGTACTTCCTTATGTATCACTTTGTGCAGAAAAGGCAGAACATCTGGAGCTACTTCTTGAACCACTTGGTAAGCGTGTTCGTAGTTATTATGGGAACCAAGGTGGTGGTACACTTCCTAAAGACACTTCTGTAGCTGTCTGCACAATAGAAAAGGCAAACTTTTTAGTAAACAAGCTGCTGGAAGAGGGCCGTCTTTCAGAAATTGGAATTGTTGTGATAGATGAACTGCACATGGTTGGCGATCCAAGTAGGGGTTATCTTCTGGAACTTCTGTTGACAAAACTTCGTTATGCAGCCGGTGAAGGCATTTCTGAATCAAGTAGTGGAGAAAGTTCAGGCATGAGCAGCGGTAAGGCTGACCCTGCTCATGGTTTGCAAATTGTTGGTATGAGTGCCACTATGCCAAATGTGGCAGCAGTTGCTGATTGGCTTCAAGCTGCCCTTTACCAGACAGAATTTCGACCGGTACCACTAGAAGAATACATCAAAGTGGGGAACACCATTTATAACAAGAAAATGGAGATTATTAGAACAATCCCAAAAGCAGTTGACCTTGGTGGTAAAGATCCAGATCATGTTGTGGAACTGTGCAATGAGGTTGTACAAGACGGTCTTTCCGTGTTAATATTTTGCTCTAGTCGAAAAGGATGTGAATCAACAGCGAGGCATGTTTCAAGATTCCTTAAGAAATTTGCAGTCAACACTCGCACTAATGACAGTGATTTTAATGACGTGACATTGGCTATTGATTCCTTACGAAGGTGTCCTGCTGGTTTGGATCCAGTATTAGAAGAAACCCTACCTTCTGGAGTTGCCTACCACCATGCTGGGCTCACGGTTGAGGAAAGAGAGATTGTTGAAACCTGCTACCGTAAAGGCCTTGTACGCGTCTTAACTGCCACATCTACCTTAGCAGCTGGTGTTAATCTGCCAGCTAGGAGAGTCATTTTTCGTCAGCCCCGGATTGGTCGCGATTTTATCGATGGGACAAGGTACAGTCAAATGGCTGGTCGGGCTGGTCGTACTGGCATAGATACAAAAGGAGAAAGTGTACTAATTTGCAAGCCAGAAGAGATCAAAAGGATTATGGGAGTTCTTAATGAAAGCTGTCTGCCATTGCGCTCTTGTTTATCTGAAGATATGAATGGAATGACTCATGCAATCTTAGAAGTTGTAGCAGGGGGAATTGTTCAAACTGCTAATGACATTCATCGGTATGTTAGGTGCACACTTTTAAACTCCACTAAACCTTTTCAGGATGTGGTGAAATCAGCACAGGAATCTCTCCGTTGGTTGTGCCACAAGAAATTTCTAGAATGGAATGACGACAATAAGTTATACAATACCACACCCCTTGGACGTGCAGCTTTTGGCAGTTCTCTGTGTCCTGAAGAATCACTTATTGTGCTGGATGATCTTTCAAGAGCAAGAGAAGGCTTTGTGCTTGCTTCTGATTTGCATCTGGTTTACCTAGTAACACCAATAAATGTTGATGTTGAGCCTGATTGGGAACTGTATTATGAACAGTTCATGGAATTGTCTGCTCTTGACCAGTCCGTTGGCAATCGCGTTGGAGTGACAGAACCATTTTTGATGCGAATGGCACATGGTGCACCTATGCGGTCATCAAACAGGATGAGAGAAAATACAAAAGCGTTGCATGGCAATTATGAAAGCCGACTTGGGACTACAAAGAGTACTGTGCTTCCGGATGATCAAACAGTTCGAGTTTGTAAACGGTTCTATGTTGCTCTCATCTTGTCAAGACTAGTTCAGGAAGTTGCTATCAATGAGGTCTGTGAAGCTTTTAAAGTGGCTAGAGGTATGGTCCAGGCCTTACAAGAGAATGCTGGAAGGTTTGCATCTATGGTAACTATGTTTTGTGAAAGGCTCGGATGGCATGATCTGGAAGGCTTGGTTGGAAAATTTCAAAACCGTGTTTCATTTGGAGTGAGAGCAGAGATTGTAGAGCTGACTACAATTCCATTTGTCAAGGGTTCTCGAGCAAGGGCACTCTATAAAGCTGGCTTGCGTACGCCTTTAGCTATTGCTGAAACATCCATCCCTGAAATTGTCAAAGCTCTTTTTGAATCTTCATCGTGGGCAGCACAAGAAGGTTCAGCTCAAAGACGCATCCAAGTAGGAGTAGCCAAGAAGATCAAGAAGGGTGCACACAAAATTGTTCTTGAAAAGGCTGAAGAGGCCAGAGTTGCTGCCTTCTCGGCTTTTAAATCACTTGGATTGGAGGTCCCACAGTTTTCTCAACCATTATTTCCAACTGCTGGTGGAAGTCCCACTATGCAAGGAACAACAAATTCCTCTGGTGATGAAAATACTAGTAGCTTTGTTAATCCCCATGTGGAGGATGCTCCTAAGCCATCTTTGGAAGGAAGGGTTCCTTCTGATAAGGTTGTATCAGGTGCGAAGTTGACAAAAACATCAGTTATTGGAACGGTGGTGGCTTCAGGAGAAGTAAATTCAAGTGCTGTCATGCAAATTAAGTTTGGGGCTGAAAATTCTGTAGTACCAATTGATGGGTCTGGTGCCCTTGCTGATGAGCTAAATGCTACTGTTCATCAAACTAAAATTGCTGACTTGACCAACTCGATTAAGGTAGACAGCTTTGGTGATAGAAATAGGATATCCAGCAAGTCTTTAGATCCTAAAAAGCAGGGAAGCTGTGAAGGGGAAAATTTGTCTGTTGGTGTTAAGGGCAATGCTTGTGAAAAAGGCCCTATTCATGCAATTTATACTCCCGGTGGAATAGATTCATTTTTGAATATTTGGGAAACCACCTCAGAGTTTCATTTTGATATCCATTACAACAAAAGATCAGAATTGAACTCTGTTGCCCCCTTTGAAATACATGGAATGGCCATCTGTTGGGAAGATTCTCCTGTGTACTATGTTAACATTCCTAAGGATCTGCTGTGTTCTGGCAGCAACAGTAAAGATGAAGGGCTGCGTTTGACTGCATCAGGTGATAGAACTAATGTGTTACCTCTTGATGATATGTTGGAGCTGGCTAAACGTAGATGGAGCAGGATTGGTGAAATCATGGGGAAAAGAGGTGTCAGGAAATTTACTTGGATGTTGAAAAGTCAGATTCAGGCACTGAAGTGCCCTGCAGTTCAAATACAGAGATTTGGTTGCCAGAGTCTTGTAGGGAAAAGTATTTGTTTTGAAATTATAGACACCTCGTTTTTATTGTTGCCACCAGTTCATATACAAGATGGCATTGACATGTGCATTGTGGCCTGGATTCTTTGGCCTGATGAGGAGAAAAATTCTAATCCAAACTTGGAAAAGGAAGTTAAGAAAAGGTTATCCAGCGACACTGCAGCAGCTGCAAATCGAAACGGCAGGTGGAAAAATCAGATGCGAAGAGCTGCACACAATGGTTGCTGCCGTCGAGTTGCTCAAACACGGGCCTTATGTTCTGTTCTGTGGAAGTTGCTTGTTTCGGAAGACCTTGCTGAGTCACTTATAAATATAGAAGTTCCATTGGTTAATATTCTTGCTGACATGGAGCTTTGGGGAGTGGGTCTTGACATGGAGGGATGTCTGCAAGCACGTAATGTGCTGGGGAAAAAGCTAAGACATCTTGAGAAAGAAGCTTATAAACTGGCTGGCATGACATTTTCATTATATACAGCTGCAGATATTGCTAATGTGCTGTATGGACACTTAAAGCTGCCCATACCAGAGGTGCGCAACAAGGGAAAACAACATCCAAGTACTGACAAGCATTGCTTGGATTTACTAAGGGACGAGCATCCTATTATTCCCATCATCAAAGAGCATCGTACATTGGCAAAACTCTTAAACTGTACGTTGGGATCAATTTGCTCACTTGCTAGGCTATCTGTGAAGACACAGAAGTACACATTACACGGTCATTGGCTCCAAACATCAACAGCTACTGGTCGGCTTTCAATGGAGGAACCTAATCTCCAGTGTGTGGAACATATGGTTGACTTCAAAATGAACAAGGATGAATATGGAGGCAAAAGTGATGCTGATCATTACAAAATTAATGCTCGAGAATACTTTATACCTACTCAGGACAACTGGATACTCTTGACCGCAGATTATTCTCAGATAGAACTGCGGCTGATGGCCCATTTTTCTGAGGATTCCTCACTCATTGAACTCCTTAGTAAGCCCCATGGTGATGTCTTCACCATGATTGCAGCTAGATGGACTGGAATGTCAGAAGATTGTATTAGTTCTGATGTACGAGATCAGACCAAAAGGTTGGTGTATGGAATTCTCTACGGAATGGGTGCTAATAGCCTTGCAGAACAACTGGATTGCAGTCCCCAGGCAGCTTCTGAGAAAATACAAAACTTCAAAAGTTCTTTCCCCGGAGTTGCTTCTTGGCTCAATGAAGCAGTTGGATATTGCCGCAAGAAAGGATATGTGGAAACCATTAAGGGAAGAAAACGATTTTTGTCTAAAATAAAATTTGGGAATAGCAAGGAAAAGTCAAAGGCGCAGAGACAGGCTGTAAATTCGATATGTCAGGGATCCGCTGCTGACATAATTAAGATTGCAATGATAAATATTTACTCCATAATAGTTGATGGAGATGAAAGGCCTGATTCGACTTCTCCACTTGCAACAAAGTTTCACAAACTGAAGGGCCGCTGCCGAATTCTATTGCAGGTACATGATGAATTGGTACTTGAAGTTGATCCTTCTGTGATAAAGGAAGCTGCTTTGCTGTTACAAACAAGCATGGAAAGTGCTGTTTCGCTTCTTGTTCCTATGCATGTCAAATTGAAAGTTGGAAGAACTTGGGGTTCTTTGCAACCCTTTCAGGCTGACCAATATGAAGATCTGCCTGATCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004647 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006563 GO:0006564 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009507 GO:0009536 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019752 GO:0022414 GO:0022622 GO:0032501 GO:0032502 GO:0042578 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046872 GO:0048229 GO:0048316 GO:0048364 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

HAD Hydrolase

Protein Analysis

2181

Amino Acids

240.28

Weight (kDa)

7.25

Isoelectric Point (pI)

42.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	525 - 701	1.1e-18	DEAD/DEAH box helicase
Helicase_C	PF00271	831 - 906	8e-09	Helicase conserved C-terminal domain
HTH_61	PF20470	911 - 1002	9.7e-25	Helix-turn-helix domain
POLQ_helical	PF21099	1026 - 1210	9.8e-53	DNA_pol_Q helicase like region helical domain
DUF7898	PF25453	1216 - 1290	2e-06	Domain of unknown function (DUF7898)
DNA_pol_A	PF00476	1753 - 2165	4.1e-110	DNA polymerase family A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005439)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18640 AT1G18640
fragaria_vesca	FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840 FvH4_5g13840
malus_domestica	MD06G1202500.v1.1 MD14G1211600.v1.1
prunus_persica	Prupe.5G205100_v2.0.a1
pyrus_communis	pycom06g17850 pycom14g17520
rosa_chinensis	RchiOBHm_Chr7g0183091
rosa_laevigata	RLG00000005079
rosa_multiflora	Rmu_sc0012218.1_g000001 Rmu_ssc0000111.1_g000006
rosa_roxburghii	Rroxscaffold_3G00270770
rosa_rugosa	Rorug06G0452700 Rorug06G0452800 Rorug06G0452800 Rorug06G0452900
rosa_samantha	Rh7AG056000 Rh7BG055300 Rh7CG056800 Rh7DG055500
rosa_wichuraiana	Rw7G004560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	180, 2196, 5177, 5310
AarI	CACCTGC	2 cut(s)	82, 5043
AasI	GACNNNNNNGTC	1 cut(s)	5899
Acc16I	TGCGCA	1 cut(s)	5402
Acc36I	ACCTGC	7 cut(s)	82, 1540, 1610, 1648, 2561, 5043, 6346
Acc65I	GGTACC	1 cut(s)	2155
AccB1I	GGYRCC	2 cut(s)	2155, 4232
AccB7I	CCANNNNNTGG	4 cut(s)	3971, 4223, 4572, 5650
AccI	GTMKAC	1 cut(s)	4305
AccII	CGCG	4 cut(s)	28, 2577, 2661, 3258
AccIII	TCCGGA	1 cut(s)	3397
AcoI	YGGCCR	1 cut(s)	4563
AcuI	CTGAAG	9 cut(s)	470, 924, 1547, 2844, 3099, 3906, 4137, 4826, 6350
AcyI	GRCGYC	1 cut(s)	5
AdeI	CACNNNGTG	6 cut(s)	131, 866, 992, 1388, 1712, 3299
AflII	CTTAAG	1 cut(s)	2372
AflIII	ACRYGT	2 cut(s)	2575, 4932
AgeI	ACCGGT	1 cut(s)	2152
AhdI	GACNNNNNGTC	2 cut(s)	472, 1000
AhlI	ACTAGT	2 cut(s)	3457, 4025
AjiI	CACGTC	2 cut(s)	2422, 3050
AjnI	CCWGG	7 cut(s)	793, 862, 915, 1384, 3519, 4946, 6012
AjuI	GAANNNNNNNTTGG	4 cut(s)	3354, 3386, 3591, 3623
AleI	CACNNNNGTG	2 cut(s)	1322, 4807
AloI	GAACNNNNNNTCC	2 cut(s)	5625, 5657
Alw21I	GWGCWC	3 cut(s)	2918, 3304, 3862
Alw26I	GTCTC	3 cut(s)	538, 771, 6184
Alw44I	GTGCAC	3 cut(s)	2914, 3300, 3858
AlwNI	CAGNNNCTG	5 cut(s)	2111, 4804, 5036, 5612, 6023
Ama87I	CYCGRG	3 cut(s)	719, 3690, 5730
Aor13HI	TCCGGA	1 cut(s)	3397
ApaLI	GTGCAC	3 cut(s)	2914, 3300, 3858
ArsI	GACNNNNNNTTYG	2 cut(s)	2683, 2715
AseI	ATTAAT	1 cut(s)	5724
AsiGI	ACCGGT	1 cut(s)	2152
Asp700I	GAANNNNTTC	2 cut(s)	636, 4456
Asp718I	GGTACC	1 cut(s)	2155
AspLEI	GCGC	4 cut(s)	30, 2811, 5403, 6187
AspS9I	GGNCC	9 cut(s)	1267, 1367, 1876, 3517, 3937, 4421, 5118, 5809, 6334
AsuC2I	CCSGG	3 cut(s)	2650, 4446, 6057
AsuII	TTCGAA	2 cut(s)	50, 272
AvaI	CYCGRG	3 cut(s)	719, 3690, 5730
AvaII	GGWCC	3 cut(s)	1267, 3517, 3937
AxyI	CCTNAGG	1 cut(s)	4608
BaeGI	GKGCMC	7 cut(s)	916, 2918, 3304, 3703, 3862, 4237, 4814
BalI	TGGCCA	1 cut(s)	4565
BamHI	GGATCC	2 cut(s)	2467, 6218
BanI	GGYRCC	2 cut(s)	2155, 4232
BanII	GRGCYC	1 cut(s)	2523
BarI	GAAGNNNNNNTAC	2 cut(s)	2472, 2504
BauI	CACGAG	1 cut(s)	1128
BbsI	GAAGAC	3 cut(s)	5163, 5567, 5860
Bbv12I	GWGCWC	3 cut(s)	2918, 3304, 3862
BceAI	ACGGC	4 cut(s)	1209, 1863, 5065, 5082
BciT130I	CCWGG	7 cut(s)	795, 864, 917, 1386, 3521, 4948, 6014
BciVI	GTATCC	2 cut(s)	1105, 5757
BclI	TGATCA	2 cut(s)	3403, 5710
BcnI	CCSGG	3 cut(s)	2650, 4446, 6057
BcoDI	GTCTC	3 cut(s)	538, 771, 6184
BcuI	ACTAGT	2 cut(s)	3457, 4025
BfmI	CTRYAG	7 cut(s)	933, 1320, 1818, 4209, 4815, 5028, 5346
BfrI	CTTAAG	1 cut(s)	2372
BfuAI	ACCTGC	7 cut(s)	82, 1540, 1610, 1648, 2561, 5043, 6346
BfuI	GTATCC	2 cut(s)	1105, 5757
BglI	GCCNNNNNGGC	1 cut(s)	3911
BglII	AGATCT	1 cut(s)	6529
BmcAI	AGTACT	3 cut(s)	1438, 3388, 5428
Bme18I	GGWCC	3 cut(s)	1267, 3517, 3937
BmeRI	GACNNNNNGTC	2 cut(s)	472, 1000
BmeT110I	CYCGRG	3 cut(s)	719, 3690, 5730
BmgBI	CACGTC	2 cut(s)	2422, 3050
BmgT120I	GGNCC	9 cut(s)	1267, 1367, 1876, 3517, 3937, 4421, 5118, 5809, 6334
BmiI	GGNNCC	9 cut(s)	1784, 2157, 2469, 3939, 4086, 4234, 5594, 5634, 6220
BpiI	GAAGAC	3 cut(s)	5163, 5567, 5860
BplI	GAGNNNNNCTC	2 cut(s)	5813, 5845
BpmI	CTGGAG	5 cut(s)	357, 414, 1754, 2517, 5627
Bpu10I	CCTNAGC	4 cut(s)	135, 360, 2599, 4062
Bpu14I	TTCGAA	2 cut(s)	50, 272
BpuEI	CTTGAG	1 cut(s)	5315
BpuMI	CCSGG	3 cut(s)	2650, 4446, 6057
Bsa29I	ATCGAT	1 cut(s)	2670
BsaAI	YACGTR	2 cut(s)	842, 5264
BsaBI	GATNNNNATC	4 cut(s)	2259, 2341, 3147, 5709
BsaHI	GRCGYC	1 cut(s)	5
BsaWI	WCCGGW	2 cut(s)	2152, 3397
BsaXI	ACNNNNNCTCC	4 cut(s)	2724, 2754, 5625, 5655
Bse118I	RCCGGY	2 cut(s)	1985, 2152
Bse21I	CCTNAGG	1 cut(s)	4608
Bse3DI	GCAATG	7 cut(s)	598, 672, 2287, 2804, 4411, 5437, 6252
Bse8I	GATNNNNATC	4 cut(s)	2259, 2341, 3147, 5709
BseAI	TCCGGA	1 cut(s)	3397
BseBI	CCWGG	7 cut(s)	795, 864, 917, 1386, 3521, 4948, 6014
BseCI	ATCGAT	1 cut(s)	2670
BseJI	GATNNNNATC	4 cut(s)	2259, 2341, 3147, 5709
BseMI	GCAATG	7 cut(s)	598, 672, 2287, 2804, 4411, 5437, 6252
BseMII	CTCAG	9 cut(s)	429, 639, 1298, 4500, 5157, 5765, 5801, 5811, 6015
BseRI	GAGGAG	4 cut(s)	9, 389, 587, 4982
BseSI	GKGCMC	7 cut(s)	916, 2918, 3304, 3703, 3862, 4237, 4814
BseYI	CCCAGC	2 cut(s)	2516, 5272
BsgI	GTGCAG	7 cut(s)	296, 1638, 1734, 1813, 1901, 3072, 5064
Bsh1236I	CGCG	4 cut(s)	28, 2577, 2661, 3258
Bsh1285I	CGRYCG	1 cut(s)	2153
BshNI	GGYRCC	2 cut(s)	2155, 4232
BshTI	ACCGGT	1 cut(s)	2152
BshVI	ATCGAT	1 cut(s)	2670
BsiEI	CGRYCG	1 cut(s)	2153
BsiHKAI	GWGCWC	3 cut(s)	2918, 3304, 3862
BsiHKCI	CYCGRG	3 cut(s)	719, 3690, 5730
BsiSI	CCGG	7 cut(s)	170, 1986, 2153, 2650, 3398, 4446, 6057
BsiWI	CGTACG	1 cut(s)	3722
BslFI	GGGAC	6 cut(s)	2689, 3388, 3923, 3966, 5471, 5994
BsmAI	GTCTC	3 cut(s)	538, 771, 6184
BsmFI	GGGAC	6 cut(s)	2689, 3388, 3923, 3966, 5471, 5994
BsmI	GAATGC	1 cut(s)	3541
BsoBI	CYCGRG	3 cut(s)	719, 3690, 5730
Bsp119I	TTCGAA	2 cut(s)	50, 272
Bsp1286I	GDGCHC	8 cut(s)	916, 2523, 2918, 3304, 3703, 3862, 4237, 4814
Bsp13I	TCCGGA	1 cut(s)	3397
Bsp1407I	TGTACA	1 cut(s)	2290
Bsp19I	CCATGG	1 cut(s)	5857
Bsp68I	TCGCGA	1 cut(s)	2661
BspCNI	CTCAG	9 cut(s)	428, 640, 1297, 4499, 5158, 5764, 5800, 5812, 6016
BspDI	ATCGAT	1 cut(s)	2670
BspEI	TCCGGA	1 cut(s)	3397
BspFNI	CGCG	4 cut(s)	28, 2577, 2661, 3258
BspLI	GGNNCC	9 cut(s)	1784, 2157, 2469, 3939, 4086, 4234, 5594, 5634, 6220
BspMAI	CTGCAG	4 cut(s)	1324, 4819, 5032, 5350
BspMI	ACCTGC	7 cut(s)	82, 1540, 1610, 1648, 2561, 5043, 6346
BspQI	GCTCTTC	3 cut(s)	618, 647, 5068
BspT104I	TTCGAA	2 cut(s)	50, 272
BspT107I	GGYRCC	2 cut(s)	2155, 4232
BspTI	CTTAAG	1 cut(s)	2372
BsrDI	GCAATG	7 cut(s)	598, 672, 2287, 2804, 4411, 5437, 6252
BsrFI	RCCGGY	2 cut(s)	1985, 2152
BsrGI	TGTACA	1 cut(s)	2290
BssAI	RCCGGY	2 cut(s)	1985, 2152
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	1128
BssT1I	CCWWGG	6 cut(s)	405, 1544, 1786, 2242, 3043, 5857
Bst2BI	CACGAG	1 cut(s)	1128
Bst2UI	CCWGG	7 cut(s)	795, 864, 917, 1386, 3521, 4948, 6014
Bst6I	CTCTTC	7 cut(s)	307, 618, 647, 1866, 2754, 3882, 5068
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	2372
BstAPI	GCANNNNNTGC	4 cut(s)	2108, 3299, 5036, 5354
BstAUI	TGTACA	1 cut(s)	2290
BstBAI	YACGTR	2 cut(s)	842, 5264
BstBI	TTCGAA	2 cut(s)	50, 272
BstDSI	CCRYGG	1 cut(s)	5857
BstEII	GGTNACC	2 cut(s)	123, 697
BstENI	CCTNNNNNAGG	1 cut(s)	2446
BstFNI	CGCG	4 cut(s)	28, 2577, 2661, 3258
BstHHI	GCGC	4 cut(s)	30, 2811, 5403, 6187
BstMAI	GTCTC	3 cut(s)	538, 771, 6184
BstMCI	CGRYCG	1 cut(s)	2153
BstNI	CCWGG	7 cut(s)	795, 864, 917, 1386, 3521, 4948, 6014
BstNSI	RCATGY	4 cut(s)	747, 2360, 4936, 6467
BstPI	GGTNACC	2 cut(s)	123, 697
BstSFI	CTRYAG	7 cut(s)	933, 1320, 1818, 4209, 4815, 5028, 5346
BstSLI	GKGCMC	7 cut(s)	916, 2918, 3304, 3703, 3862, 4237, 4814
BstUI	CGCG	4 cut(s)	28, 2577, 2661, 3258
BstV2I	GAAGAC	3 cut(s)	5163, 5567, 5860
BstX2I	RGATCY	6 cut(s)	2254, 2467, 4348, 4612, 6218, 6529
BstXI	CCANNNNNNTGG	5 cut(s)	956, 1793, 2516, 3248, 5947
BstYI	RGATCY	6 cut(s)	2254, 2467, 4348, 4612, 6218, 6529
Bsu15I	ATCGAT	1 cut(s)	2670
Bsu36I	CCTNAGG	1 cut(s)	4608
BsuI	GTATCC	2 cut(s)	1105, 5757
BsuTUI	ATCGAT	1 cut(s)	2670
BtgI	CCRYGG	1 cut(s)	5857
BtgZI	GCGATG	1 cut(s)	150
BtrI	CACGTC	2 cut(s)	2422, 3050
BtsI	GCAGTG	6 cut(s)	493, 1100, 1317, 1329, 1457, 5025
BtuMI	TCGCGA	1 cut(s)	2661
BveI	ACCTGC	7 cut(s)	82, 1540, 1610, 1648, 2561, 5043, 6346
CaiI	CAGNNNCTG	5 cut(s)	2111, 4804, 5036, 5612, 6023
CfoI	GCGC	4 cut(s)	30, 2811, 5403, 6187
Cfr10I	RCCGGY	2 cut(s)	1985, 2152
Cfr13I	GGNCC	9 cut(s)	1267, 1367, 1876, 3517, 3937, 4421, 5118, 5809, 6334
ClaI	ATCGAT	1 cut(s)	2670
CseI	GACGC	2 cut(s)	2566, 3828
CsiI	ACCWGGT	2 cut(s)	862, 1384
CspAI	ACCGGT	1 cut(s)	2152
CspCI	CAANNNNNGTGG	2 cut(s)	3025, 3060
DraI	TTTAAA	3 cut(s)	2925, 3499, 3919
DraIII	CACNNNGTG	6 cut(s)	131, 866, 992, 1388, 1712, 3299
DrdI	GACNNNNNNGTC	1 cut(s)	5899
DriI	GACNNNNNGTC	2 cut(s)	472, 1000
DseDI	GACNNNNNNGTC	1 cut(s)	5899
EaeI	YGGCCR	1 cut(s)	4563
Eam1104I	CTCTTC	7 cut(s)	307, 618, 647, 1866, 2754, 3882, 5068
Eam1105I	GACNNNNNGTC	2 cut(s)	472, 1000
EarI	CTCTTC	7 cut(s)	307, 618, 647, 1866, 2754, 3882, 5068
Eco130I	CCWWGG	6 cut(s)	405, 1544, 1786, 2242, 3043, 5857
Eco147I	AGGCCT	3 cut(s)	2568, 3524, 6289
Eco24I	GRGCYC	1 cut(s)	2523
Eco32I	GATATC	2 cut(s)	4332, 4504
Eco47I	GGWCC	3 cut(s)	1267, 3517, 3937
Eco57I	CTGAAG	9 cut(s)	470, 924, 1547, 2844, 3099, 3906, 4137, 4826, 6350
Eco81I	CCTNAGG	1 cut(s)	4608
Eco88I	CYCGRG	3 cut(s)	719, 3690, 5730
Eco91I	GGTNACC	2 cut(s)	123, 697
EcoNI	CCTNNNNNAGG	1 cut(s)	2446
EcoO109I	RGGNCCY	2 cut(s)	3937, 4421
EcoO65I	GGTNACC	2 cut(s)	123, 697
EcoRI	GAATTC	2 cut(s)	5958, 6345
EcoRII	CCWGG	7 cut(s)	793, 862, 915, 1384, 3519, 4946, 6012
EcoRV	GATATC	2 cut(s)	4332, 4504
EcoT14I	CCWWGG	6 cut(s)	405, 1544, 1786, 2242, 3043, 5857
EcoT22I	ATGCAT	1 cut(s)	6465
EcoT38I	GRGCYC	1 cut(s)	2523
ErhI	CCWWGG	6 cut(s)	405, 1544, 1786, 2242, 3043, 5857
FaqI	GGGAC	6 cut(s)	2689, 3388, 3923, 3966, 5471, 5994
FauNDI	CATATG	1 cut(s)	5658
FbaI	TGATCA	2 cut(s)	3403, 5710
FblI	GTMKAC	1 cut(s)	4305
FriOI	GRGCYC	1 cut(s)	2523
FspI	TGCGCA	1 cut(s)	5402
GlaI	GCGC	4 cut(s)	29, 2810, 5402, 6186
GsaI	CCCAGC	2 cut(s)	2520, 5276
GsuI	CTGGAG	5 cut(s)	357, 414, 1754, 2517, 5627
HapII	CCGG	7 cut(s)	170, 1986, 2153, 2650, 3398, 4446, 6057
HgaI	GACGC	2 cut(s)	2566, 3828
HhaI	GCGC	4 cut(s)	30, 2811, 5403, 6187
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	4 cut(s)	28, 2809, 5401, 6185
HinP1I	GCGC	4 cut(s)	28, 2809, 5401, 6185
HincII	GTYRAC	6 cut(s)	1965, 2239, 2389, 4119, 4600, 5665
HindII	GTYRAC	6 cut(s)	1965, 2239, 2389, 4119, 4600, 5665
HindIII	AAGCTT	4 cut(s)	870, 1535, 3492, 5304
HpaI	GTTAAC	1 cut(s)	4600
HpaII	CCGG	7 cut(s)	170, 1986, 2153, 2650, 3398, 4446, 6057
Hpy99I	CGWCG	2 cut(s)	28, 5103
HpyCH4IV	ACGT	6 cut(s)	841, 2421, 3049, 4720, 5263, 5522
HpySE526I	ACGT	6 cut(s)	841, 2421, 3049, 4720, 5263, 5522
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	4 cut(s)	28, 2809, 5401, 6185
Kpn2I	TCCGGA	1 cut(s)	3397
KpnI	GGTACC	1 cut(s)	2159
Ksp22I	TGATCA	2 cut(s)	3403, 5710
KspAI	GTTAAC	1 cut(s)	4600
LguI	GCTCTTC	3 cut(s)	618, 647, 5068
LmnI	GCTCC	7 cut(s)	338, 1735, 4063, 4708, 4728, 5218, 5598
MabI	ACCWGGT	2 cut(s)	862, 1384
MaeII	ACGT	6 cut(s)	841, 2421, 3049, 4720, 5263, 5522
MfeI	CAATTG	1 cut(s)	4218
MflI	RGATCY	6 cut(s)	2254, 2467, 4348, 4612, 6218, 6529
MhlI	GDGCHC	8 cut(s)	916, 2523, 2918, 3304, 3703, 3862, 4237, 4814
MlsI	TGGCCA	1 cut(s)	4565
MluI	ACGCGT	1 cut(s)	2575
MluNI	TGGCCA	1 cut(s)	4565
MlyI	GAGTC	4 cut(s)	2640, 2836, 4855, 5177
MmeI	TCCRAC	6 cut(s)	3241, 3589, 3989, 4686, 6067, 6460
Mox20I	TGGCCA	1 cut(s)	4565
Mph1103I	ATGCAT	1 cut(s)	6465
MroI	TCCGGA	1 cut(s)	3397
MroXI	GAANNNNTTC	2 cut(s)	636, 4456
MscI	TGGCCA	1 cut(s)	4565
MslI	CAYNNNNRTG	8 cut(s)	318, 1203, 1322, 2177, 2904, 4807, 4931, 5862
Msp20I	TGGCCA	1 cut(s)	4565
MspA1I	CMGCKG	8 cut(s)	218, 392, 2037, 2606, 5036, 5345, 6224, 6339
MspCI	CTTAAG	1 cut(s)	2372
MspI	CCGG	7 cut(s)	170, 1986, 2153, 2650, 3398, 4446, 6057
MunI	CAATTG	1 cut(s)	4218
Mva1269I	GAATGC	1 cut(s)	3541
MvaI	CCWGG	7 cut(s)	795, 864, 917, 1386, 3521, 4948, 6014
MvnI	CGCG	4 cut(s)	28, 2577, 2661, 3258
NciI	CCSGG	3 cut(s)	2650, 4446, 6057
NcoI	CCATGG	1 cut(s)	5857
NdeI	CATATG	1 cut(s)	5658
NlaIV	GGNNCC	9 cut(s)	1784, 2157, 2469, 3939, 4086, 4234, 5594, 5634, 6220
NmeAIII	GCCGAG	2 cut(s)	202, 3890
NmuCI	GTSAC	7 cut(s)	123, 992, 1315, 1324, 2422, 3265, 5169
NruI	TCGCGA	1 cut(s)	2661
NsbI	TGCGCA	1 cut(s)	5402
NsiI	ATGCAT	1 cut(s)	6465
NspI	RCATGY	4 cut(s)	747, 2360, 4936, 6467
NspV	TTCGAA	2 cut(s)	50, 272
OliI	CACNNNNGTG	2 cut(s)	1322, 4807
PaeR7I	CTCGAG	3 cut(s)	719, 3690, 5730
PaqCI	CACCTGC	2 cut(s)	82, 5043
PceI	AGGCCT	3 cut(s)	2568, 3524, 6289
PciI	ACATGT	1 cut(s)	4932
PciSI	GCTCTTC	3 cut(s)	618, 647, 5068
PcsI	WCGNNNNNNNCGW	3 cut(s)	20, 2304, 2707
PctI	GAATGC	1 cut(s)	3541
PdmI	GAANNNNTTC	2 cut(s)	636, 4456
Pfl23II	CGTACG	1 cut(s)	3722
PflMI	CCANNNNNTGG	4 cut(s)	3971, 4223, 4572, 5650
PinAI	ACCGGT	1 cut(s)	2152
PleI	GAGTC	4 cut(s)	2639, 2836, 4854, 5176
PpsI	GAGTC	4 cut(s)	2639, 2836, 4854, 5176
Ppu21I	YACGTR	2 cut(s)	842, 5264
PpuMI	RGGWCCY	1 cut(s)	3937
PscI	ACATGT	1 cut(s)	4932
PshBI	ATTAAT	1 cut(s)	5724
PsiI	TTATAA	4 cut(s)	180, 2196, 5177, 5310
Psp5II	RGGWCCY	1 cut(s)	3937
Psp6I	CCWGG	7 cut(s)	793, 862, 915, 1384, 3519, 4946, 6012
PspEI	GGTNACC	2 cut(s)	123, 697
PspFI	CCCAGC	2 cut(s)	2516, 5272
PspGI	CCWGG	7 cut(s)	793, 862, 915, 1384, 3519, 4946, 6012
PspLI	CGTACG	1 cut(s)	3722
PspN4I	GGNNCC	9 cut(s)	1784, 2157, 2469, 3939, 4086, 4234, 5594, 5634, 6220
PspPI	GGNCC	9 cut(s)	1267, 1367, 1876, 3517, 3937, 4421, 5118, 5809, 6334
PspPPI	RGGWCCY	1 cut(s)	3937
PspXI	VCTCGAGB	1 cut(s)	719
PstI	CTGCAG	4 cut(s)	1324, 4819, 5032, 5350
PstNI	CAGNNNCTG	5 cut(s)	2111, 4804, 5036, 5612, 6023
PsuI	RGATCY	6 cut(s)	2254, 2467, 4348, 4612, 6218, 6529
PvuII	CAGCTG	4 cut(s)	392, 2606, 5036, 5345
RruI	TCGCGA	1 cut(s)	2661
RseI	CAYNNNNRTG	8 cut(s)	318, 1203, 1322, 2177, 2904, 4807, 4931, 5862
SapI	GCTCTTC	3 cut(s)	618, 647, 5068
Sau96I	GGNCC	9 cut(s)	1267, 1367, 1876, 3517, 3937, 4421, 5118, 5809, 6334
ScaI	AGTACT	3 cut(s)	1438, 3388, 5428
SchI	GAGTC	4 cut(s)	2640, 2836, 4855, 5177
SduI	GDGCHC	8 cut(s)	916, 2523, 2918, 3304, 3703, 3862, 4237, 4814
SexAI	ACCWGGT	2 cut(s)	862, 1384
SfcI	CTRYAG	7 cut(s)	933, 1320, 1818, 4209, 4815, 5028, 5346
Sfr274I	CTCGAG	3 cut(s)	719, 3690, 5730
SfuI	TTCGAA	2 cut(s)	50, 272
SinI	GGWCC	3 cut(s)	1267, 3517, 3937
SlaI	CTCGAG	3 cut(s)	719, 3690, 5730
SmiMI	CAYNNNNRTG	8 cut(s)	318, 1203, 1322, 2177, 2904, 4807, 4931, 5862
SmlI	CTYRAG	5 cut(s)	719, 2372, 3690, 5294, 5730
SmoI	CTYRAG	5 cut(s)	719, 2372, 3690, 5294, 5730
SpeI	ACTAGT	2 cut(s)	3457, 4025
SseBI	AGGCCT	3 cut(s)	2568, 3524, 6289
SspI	AATATT	4 cut(s)	2316, 4471, 5203, 6256
StuI	AGGCCT	3 cut(s)	2568, 3524, 6289
StyI	CCWWGG	6 cut(s)	405, 1544, 1786, 2242, 3043, 5857
TaiI	ACGT	6 cut(s)	844, 2424, 3052, 4723, 5266, 5525
TatI	WGTACW	8 cut(s)	1436, 1690, 2290, 2740, 3386, 4590, 5426, 5571
TauI	GCSGC	5 cut(s)	221, 1657, 5803, 6099, 6339
TseFI	GTSAC	7 cut(s)	123, 992, 1315, 1324, 2422, 3265, 5169
Tsp45I	GTSAC	7 cut(s)	123, 992, 1315, 1324, 2422, 3265, 5169
TspGWI	ACGGA	7 cut(s)	12, 73, 1259, 2296, 2966, 3235, 5982
Van91I	CCANNNNNTGG	4 cut(s)	3971, 4223, 4572, 5650
Vha464I	CTTAAG	1 cut(s)	2372
VneI	GTGCAC	3 cut(s)	2914, 3300, 3858
VpaK11BI	GGWCC	3 cut(s)	1267, 3517, 3937
VspI	ATTAAT	1 cut(s)	5724
XagI	CCTNNNNNAGG	1 cut(s)	2446
XbaI	TCTAGA	3 cut(s)	769, 1477, 3001
XceI	RCATGY	4 cut(s)	747, 2360, 4936, 6467
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1336
XhoI	CTCGAG	3 cut(s)	719, 3690, 5730
XmiI	GTMKAC	1 cut(s)	4305
XmnI	GAANNNNTTC	2 cut(s)	636, 4456
ZrmI	AGTACT	3 cut(s)	1438, 3388, 5428
Zsp2I	ATGCAT	1 cut(s)	6465

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.