Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006824

Physical Location & Seq

Genomic Coordinates Reverse (-)

29750055 .. 29754987

Gene Structure 4933 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1212900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGCAGGAAGCGACTCCTGCCCCTGCTCCCAGTGCCCAACTTGTTGGCAATGCCTTCGTGGAGCAATATTACCATATTCTTCACCAATCTCCAGAGTTAGTGCATAGGTTTTATCAGGATTCGAGCTCTCTGAGCAGGATGGATATCAAGGGCAATATGACAACAGTGACAACCATGGAAGCAATAGGTGAAAAAATTCAGTCCTTAAATTATGGAGACTATACGGCAGAAATAAAAACAGCAGATTCTCAGGAGTCTTATGAGAAAGGGGTGATTGTTCTCGTGACTGGATGCTTGACTGGAAAGGACAATGTGGGAAGAAAATTCGCGCAGACATTCTTTCTAGCTCCACAGGAGAAAGGGTACTATGTGTTGAATGATGTTTTTAGGTATATTGAGGACAACGAATCGTTGCCGACCAATACTGCCTCAGTCAATGGCATCAACGAAAGTGCTCCAGAAGCTATTGTGACAGCAGAACCAGAGCCTATTCATGCACCTGATCATCTTGTGGTGGACCCTGCAATTCCCTTTGAGGAAGAAGATCTCAATAATGGTGCTGAAGTTTGTGATCCTTCAGATAATGACGAAGGATCAGTTGTTGAAGATGAGCTTGTGGAGCCCCCAGCTCTCCCAAGTCAGAATCTTGCAGAAGTTGATCCAACTCCTGATCCTGCCCCTGAAACTCAGGAGGATGCTCCAAAGAAATCATATGCATCAATTTTATTGAAGAAAAGCATGGCCAGTCCTGTCCGTGTGGTTCCCCACACTGTGAGGACAGTATCTGCAAACACTGACCATCTACCTGCCGGTCCTGCAAAATCATTTCCTGCACCAGAGGCATCACCTCCTACAGGTGACGCTGCCCCTGAAAGCAGCCATTCTCATGAGGAAGCGCCTGAAGGTTACTCCATACATGTACGGAATTTGCCGTATGATGCAACAGTGGGACAACTTGAGAAGGAGTTCAAGAGGTTTGGACCTATTAAGCGTGATGGGATCCAAGTCAGGAGTAGTAAGCAAGGATTCTGTTTTGGCTTCGTTGAATTCGAAAGCTTGAGTTCCATGCAAAGTGCACTTGAGGCCTCACCTATCACCATTGGGGATCGCCCAGCAGTGATTGAGGAAAAGAGAACCTCAACTCGAGTTAGTGGTGGGAGAGGGAGGTTCGCTTCAGGAAGAGCTGGCTATCGGAATGACAATTTCAGGGGTCGTGGGAACTATGGTGGTGGCGGCCGTAGCTTTGGCAGGAATGAATTCAGAAACCAAGGTGAGTTTTCTGGGCGAGCCAGGGGCTCGGGAGGACGCAGTGGAGATGGTTATCAGCGGGGGAGTTCCAATGGAAGAGGTGGGCGTCAAGGTGGCGGAAACCGCAGTTCTGTTCCCCCTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006508 GO:0006807 GO:0006914 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0010494 GO:0016236 GO:0016579 GO:0019538 GO:0032386 GO:0032879 GO:0032991 GO:0034517 GO:0035770 GO:0035973 GO:0036211 GO:0036464 GO:0043021 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0051049 GO:0060341 GO:0060627 GO:0060628 GO:0061912 GO:0061919 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1905538 GO:1990861 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTF2 RRM_1

Protein Analysis

465

Amino Acids

50.01

Weight (kDa)

5.11

Isoelectric Point (pI)

54.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NTF2	PF02136	15 - 131	7.2e-34	Nuclear transport factor 2 (NTF2) domain
RRM_1	PF00076	306 - 362	1.9e-10	RNA recognition motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006824)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60980 AT5G60980 AT5G60980 AT5G60980
fragaria_vesca	FvH4_5g13730 FvH4_5g13730 FvH4_5g13730
malus_domestica	MD06G1226000.v1.1 MD14G1212900.v1.1
prunus_persica	Prupe.5G206200_v2.0.a1
pyrus_communis	pycom06g18040 pycom14g17650
rosa_chinensis	RchiOBHm_Chr7g0183211
rosa_laevigata	RLG00000005066
rosa_multiflora	Rmu_ssc0000111.1_g000020
rosa_roxburghii	Rroxscaffold_3G00270650
rosa_rugosa	Rorug06G0454900 Rorug06G0455000 Rorug06G0455100 Rorug06G0455200 Rorug06G0455200
rosa_samantha	Rh7AG057100 Rh7BG057000 Rh7CG058100 Rh7DG056900
rosa_wichuraiana	Rw7G004680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	814
AccII	CGCG	1 cut(s)	329
AciI	CCGC	4 cut(s)	1233, 1327, 1365, 1372
AclWI	GGATC	7 cut(s)	566, 601, 653, 665, 994, 1007, 1113
AcoI	YGGCCR	2 cut(s)	741, 1234
AcsI	RAATTY	5 cut(s)	195, 323, 925, 1046, 1256
AcuI	CTGAAG	4 cut(s)	561, 582, 921, 1158
AcyI	GRCGYC	1 cut(s)	1354
AdeI	CACNNNGTG	1 cut(s)	772
AfaI	GTAC	2 cut(s)	365, 921
AfiI	CCNNNNNNNGG	1 cut(s)	854
AflIII	ACRYGT	1 cut(s)	916
AgsI	TTSAA	5 cut(s)	376, 605, 730, 970, 1046
AjnI	CCWGG	1 cut(s)	1289
AluBI	AGCT	8 cut(s)	126, 347, 464, 613, 629, 1056, 1184, 1242
AluI	AGCT	8 cut(s)	126, 347, 464, 613, 629, 1056, 1184, 1242
Alw21I	GWGCWC	3 cut(s)	128, 457, 1078
Alw26I	GTCTC	1 cut(s)	210
Alw44I	GTGCAC	1 cut(s)	1074
AlwI	GGATC	7 cut(s)	566, 601, 653, 665, 994, 1007, 1113
AlwNI	CAGNNNCTG	1 cut(s)	785
Ama87I	CYCGRG	2 cut(s)	1143, 1297
AoxI	GGCC	3 cut(s)	741, 1083, 1234
ApaLI	GTGCAC	1 cut(s)	1074
ApeKI	GCWGC	2 cut(s)	863, 876
ApoI	RAATTY	5 cut(s)	195, 323, 925, 1046, 1256
Asp700I	GAANNNNTTC	3 cut(s)	195, 965, 1256
AspLEI	GCGC	2 cut(s)	331, 898
AspS9I	GGNCC	3 cut(s)	517, 812, 980
AsuHPI	GGTGA	8 cut(s)	74, 200, 283, 837, 869, 1080, 1087, 1283
AsuII	TTCGAA	1 cut(s)	1050
AvaI	CYCGRG	2 cut(s)	1143, 1297
AvaII	GGWCC	3 cut(s)	517, 812, 980
BaeGI	GKGCMC	2 cut(s)	37, 1078
BalI	TGGCCA	1 cut(s)	743
BamHI	GGATCC	1 cut(s)	999
BanII	GRGCYC	3 cut(s)	128, 624, 1298
BauI	CACGAG	1 cut(s)	281
Bbv12I	GWGCWC	3 cut(s)	128, 457, 1078
BbvI	GCAGC	2 cut(s)	850, 888
BccI	CCATC	4 cut(s)	133, 807, 989, 1310
BceAI	ACGGC	3 cut(s)	240, 916, 1221
BcgI	CGANNNNNNTGC	2 cut(s)	37, 71
BciT130I	CCWGG	1 cut(s)	1291
BclI	TGATCA	1 cut(s)	502
BcoDI	GTCTC	1 cut(s)	210
BfaI	CTAG	1 cut(s)	344
BfmI	CTRYAG	1 cut(s)	852
BfoI	RGCGCY	1 cut(s)	899
BfuAI	ACCTGC	1 cut(s)	814
BglII	AGATCT	1 cut(s)	544
BisI	GCNGC	3 cut(s)	864, 877, 1234
BlsI	GCNGC	3 cut(s)	865, 878, 1235
Bme1390I	CCNGG	1 cut(s)	1291
Bme18I	GGWCC	3 cut(s)	517, 812, 980
BmeT110I	CYCGRG	2 cut(s)	1143, 1297
BmgT120I	GGNCC	3 cut(s)	517, 812, 980
BmiI	GGNNCC	4 cut(s)	519, 621, 762, 1001
BmrFI	CCNGG	1 cut(s)	1291
BmrI	ACTGGG	1 cut(s)	24
BmsI	GCATC	6 cut(s)	281, 450, 685, 725, 851, 928
BmuI	ACTGGG	1 cut(s)	24
BpmI	CTGGAG	2 cut(s)	75, 441
Bpu14I	TTCGAA	1 cut(s)	1050
BpuEI	CTTGAG	3 cut(s)	977, 1078, 1100
BsaBI	GATNNNNATC	2 cut(s)	143, 1320
BsaHI	GRCGYC	1 cut(s)	1354
BsaJI	CCNNGG	3 cut(s)	174, 1267, 1290
BsaXI	ACNNNNNCTCC	2 cut(s)	347, 377
Bsc4I	CCNNNNNNNGG	1 cut(s)	854
Bse118I	RCCGGY	1 cut(s)	809
Bse1I	ACTGG	4 cut(s)	30, 292, 304, 744
Bse3DI	GCAATG	1 cut(s)	55
Bse8I	GATNNNNATC	2 cut(s)	143, 1320
BseBI	CCWGG	1 cut(s)	1291
BseDI	CCNNGG	3 cut(s)	174, 1267, 1290
BseGI	GGATG	3 cut(s)	144, 296, 700
BseJI	GATNNNNATC	2 cut(s)	143, 1320
BseLI	CCNNNNNNNGG	1 cut(s)	854
BseMI	GCAATG	1 cut(s)	55
BseMII	CTCAG	4 cut(s)	122, 263, 444, 701
BseNI	ACTGG	4 cut(s)	30, 292, 304, 744
BseSI	GKGCMC	2 cut(s)	37, 1078
BseX3I	CGGCCG	1 cut(s)	1234
BseXI	GCAGC	2 cut(s)	850, 888
BseYI	CCCAGC	2 cut(s)	625, 1111
BsgI	GTGCAG	1 cut(s)	816
Bsh1236I	CGCG	1 cut(s)	329
Bsh1285I	CGRYCG	1 cut(s)	1237
BshFI	GGCC	3 cut(s)	743, 1085, 1236
BsiEI	CGRYCG	1 cut(s)	1237
BsiHKAI	GWGCWC	3 cut(s)	128, 457, 1078
BsiHKCI	CYCGRG	2 cut(s)	1143, 1297
BsiSI	CCGG	1 cut(s)	810
BslFI	GGGAC	1 cut(s)	963
BslI	CCNNNNNNNGG	1 cut(s)	854
BsmAI	GTCTC	1 cut(s)	210
BsmFI	GGGAC	1 cut(s)	963
BsnI	GGCC	3 cut(s)	743, 1085, 1236
BsoBI	CYCGRG	2 cut(s)	1143, 1297
Bsp119I	TTCGAA	1 cut(s)	1050
Bsp1286I	GDGCHC	6 cut(s)	37, 128, 457, 624, 1078, 1298
Bsp143I	GATC	8 cut(s)	502, 544, 571, 593, 658, 670, 999, 1105
Bsp19I	CCATGG	1 cut(s)	174
BspACI	CCGC	4 cut(s)	1233, 1327, 1365, 1372
BspANI	GGCC	3 cut(s)	743, 1085, 1236
BspCNI	CTCAG	4 cut(s)	123, 262, 443, 700
BspFNI	CGCG	1 cut(s)	329
BspHI	TCATGA	1 cut(s)	886
BspLI	GGNNCC	4 cut(s)	519, 621, 762, 1001
BspMI	ACCTGC	1 cut(s)	814
BspPI	GGATC	7 cut(s)	566, 601, 653, 665, 994, 1007, 1113
BspQI	GCTCTTC	1 cut(s)	1174
BspT104I	TTCGAA	1 cut(s)	1050
BsrDI	GCAATG	1 cut(s)	55
BsrFI	RCCGGY	1 cut(s)	809
BsrI	ACTGG	4 cut(s)	30, 292, 304, 744
BssAI	RCCGGY	1 cut(s)	809
BssECI	CCNNGG	3 cut(s)	174, 1267, 1290
BssMI	GATC	8 cut(s)	502, 544, 571, 593, 658, 670, 999, 1105
BssNI	GRCGYC	1 cut(s)	1354
BssSI	CACGAG	1 cut(s)	281
BssT1I	CCWWGG	2 cut(s)	174, 1267
Bst2BI	CACGAG	1 cut(s)	281
Bst2UI	CCWGG	1 cut(s)	1291
Bst4CI	ACNGT	4 cut(s)	166, 772, 781, 946
Bst6I	CTCTTC	2 cut(s)	1174, 1339
BstACI	GRCGYC	1 cut(s)	1354
BstBI	TTCGAA	1 cut(s)	1050
BstC8I	GCNNGC	2 cut(s)	1186, 1287
BstDEI	CTNAG	4 cut(s)	131, 249, 430, 687
BstDSI	CCRYGG	1 cut(s)	174
BstENI	CCTNNNNNAGG	1 cut(s)	852
BstF5I	GGATG	3 cut(s)	144, 296, 700
BstFNI	CGCG	1 cut(s)	329
BstH2I	RGCGCY	1 cut(s)	899
BstHHI	GCGC	2 cut(s)	331, 898
BstKTI	GATC	8 cut(s)	505, 547, 574, 596, 661, 673, 1002, 1108
BstMAI	GTCTC	1 cut(s)	210
BstMBI	GATC	8 cut(s)	502, 544, 571, 593, 658, 670, 999, 1105
BstMCI	CGRYCG	1 cut(s)	1237
BstNI	CCWGG	1 cut(s)	1291
BstNSI	RCATGY	1 cut(s)	920
BstSCI	CCNGG	1 cut(s)	1289
BstSFI	CTRYAG	1 cut(s)	852
BstSLI	GKGCMC	2 cut(s)	37, 1078
BstUI	CGCG	1 cut(s)	329
BstV1I	GCAGC	2 cut(s)	850, 888
BstX2I	RGATCY	2 cut(s)	544, 999
BstXI	CCANNNNNNTGG	1 cut(s)	44
BstYI	RGATCY	2 cut(s)	544, 999
BstZI	CGGCCG	1 cut(s)	1234
BsuRI	GGCC	3 cut(s)	743, 1085, 1236
BtgI	CCRYGG	1 cut(s)	174
BtsCI	GGATG	3 cut(s)	144, 296, 700
BtsI	GCAGTG	2 cut(s)	1122, 1315
BtsIMutI	CAGTG	7 cut(s)	37, 171, 768, 792, 951, 1122, 1315
BveI	ACCTGC	1 cut(s)	814
Cac8I	GCNNGC	2 cut(s)	1186, 1287
CaiI	CAGNNNCTG	1 cut(s)	785
CciI	TCATGA	1 cut(s)	886
CfoI	GCGC	2 cut(s)	331, 898
Cfr10I	RCCGGY	1 cut(s)	809
Cfr13I	GGNCC	3 cut(s)	517, 812, 980
CseI	GACGC	3 cut(s)	869, 1314, 1343
Csp6I	GTAC	2 cut(s)	364, 920
CviAII	CATG	6 cut(s)	175, 494, 739, 887, 917, 1066
CviQI	GTAC	2 cut(s)	364, 920
DdeI	CTNAG	4 cut(s)	131, 249, 430, 687
DpnI	GATC	8 cut(s)	504, 546, 573, 595, 660, 672, 1001, 1107
DpnII	GATC	8 cut(s)	502, 544, 571, 593, 658, 670, 999, 1105
DraIII	CACNNNGTG	1 cut(s)	772
EaeI	YGGCCR	2 cut(s)	741, 1234
EagI	CGGCCG	1 cut(s)	1234
Eam1104I	CTCTTC	2 cut(s)	1174, 1339
EarI	CTCTTC	2 cut(s)	1174, 1339
EciI	GGCGGA	1 cut(s)	1380
Ecl136II	GAGCTC	1 cut(s)	126
EclXI	CGGCCG	1 cut(s)	1234
Eco130I	CCWWGG	2 cut(s)	174, 1267
Eco147I	AGGCCT	1 cut(s)	1085
Eco24I	GRGCYC	3 cut(s)	128, 624, 1298
Eco32I	GATATC	1 cut(s)	145
Eco47I	GGWCC	3 cut(s)	517, 812, 980
Eco52I	CGGCCG	1 cut(s)	1234
Eco53kI	GAGCTC	1 cut(s)	126
Eco57I	CTGAAG	4 cut(s)	561, 582, 921, 1158
Eco88I	CYCGRG	2 cut(s)	1143, 1297
EcoICRI	GAGCTC	1 cut(s)	126
EcoNI	CCTNNNNNAGG	1 cut(s)	852
EcoRI	GAATTC	2 cut(s)	1046, 1256
EcoRII	CCWGG	1 cut(s)	1289
EcoRV	GATATC	1 cut(s)	145
EcoT14I	CCWWGG	2 cut(s)	174, 1267
EcoT22I	ATGCAT	1 cut(s)	718
EcoT38I	GRGCYC	3 cut(s)	128, 624, 1298
ErhI	CCWWGG	2 cut(s)	174, 1267
FaeI	CATG	6 cut(s)	178, 497, 742, 890, 920, 1069
FalI	AAGNNNNNCTT	2 cut(s)	597, 629
FaqI	GGGAC	1 cut(s)	963
FatI	CATG	6 cut(s)	174, 493, 738, 886, 916, 1065
FauI	CCCGC	1 cut(s)	1320
FauNDI	CATATG	1 cut(s)	712
FbaI	TGATCA	1 cut(s)	502
Fnu4HI	GCNGC	3 cut(s)	864, 877, 1234
FokI	GGATG	3 cut(s)	151, 303, 707
FriOI	GRGCYC	3 cut(s)	128, 624, 1298
Fsp4HI	GCNGC	3 cut(s)	864, 877, 1234
FspBI	CTAG	1 cut(s)	344
GlaI	GCGC	2 cut(s)	330, 897
GluI	GCNGC	3 cut(s)	864, 877, 1234
GsaI	CCCAGC	2 cut(s)	629, 1115
GsuI	CTGGAG	2 cut(s)	75, 441
HaeII	RGCGCY	1 cut(s)	899
HaeIII	GGCC	3 cut(s)	743, 1085, 1236
HapII	CCGG	1 cut(s)	810
HgaI	GACGC	3 cut(s)	869, 1314, 1343
HhaI	GCGC	2 cut(s)	331, 898
Hin1I	GRCGYC	1 cut(s)	1354
Hin1II	CATG	6 cut(s)	178, 497, 742, 890, 920, 1069
Hin6I	GCGC	2 cut(s)	329, 896
HinP1I	GCGC	2 cut(s)	329, 896
HindIII	AAGCTT	1 cut(s)	1054
HinfI	GANTC	7 cut(s)	13, 119, 245, 254, 407, 643, 1026
HpaII	CCGG	1 cut(s)	810
HphI	GGTGA	8 cut(s)	74, 200, 283, 837, 869, 1080, 1087, 1283
Hpy166II	GTNNAC	2 cut(s)	517, 1076
Hpy188I	TCNGA	5 cut(s)	132, 580, 642, 1194, 1262
Hpy8I	GTNNAC	2 cut(s)	517, 1076
HpyAV	CCTTC	5 cut(s)	64, 584, 585, 896, 955
HpyCH4III	ACNGT	4 cut(s)	166, 772, 781, 946
HpyF3I	CTNAG	4 cut(s)	131, 249, 430, 687
Hsp92I	GRCGYC	1 cut(s)	1354
Hsp92II	CATG	6 cut(s)	178, 497, 742, 890, 920, 1069
HspAI	GCGC	2 cut(s)	329, 896
Ksp22I	TGATCA	1 cut(s)	502
Kzo9I	GATC	8 cut(s)	502, 544, 571, 593, 658, 670, 999, 1105
LguI	GCTCTTC	1 cut(s)	1174
LmnI	GCTCC	6 cut(s)	31, 61, 352, 460, 619, 703
Lsp1109I	GCAGC	2 cut(s)	850, 888
LweI	GCATC	6 cut(s)	281, 450, 685, 725, 851, 928
MaeI	CTAG	1 cut(s)	344
MaeIII	GTNAC	5 cut(s)	166, 283, 469, 857, 905
MalI	GATC	8 cut(s)	504, 546, 573, 595, 660, 672, 1001, 1107
MboI	GATC	8 cut(s)	502, 544, 571, 593, 658, 670, 999, 1105
MboII	GAAGA	8 cut(s)	71, 330, 551, 554, 617, 742, 1191, 1356
MflI	RGATCY	2 cut(s)	544, 999
MhlI	GDGCHC	6 cut(s)	37, 128, 457, 624, 1078, 1298
MlsI	TGGCCA	1 cut(s)	743
MluCI	AATT	9 cut(s)	195, 208, 323, 525, 720, 925, 1046, 1201, 1256
MluNI	TGGCCA	1 cut(s)	743
MlyI	GAGTC	2 cut(s)	7, 263
MmeI	TCCRAC	1 cut(s)	686
Mox20I	TGGCCA	1 cut(s)	743
Mph1103I	ATGCAT	1 cut(s)	718
MroXI	GAANNNNTTC	3 cut(s)	195, 965, 1256
MscI	TGGCCA	1 cut(s)	743
MseI	TTAA	2 cut(s)	206, 987
MslI	CAYNNNNRTG	1 cut(s)	885
Msp20I	TGGCCA	1 cut(s)	743
MspA1I	CMGCKG	1 cut(s)	1327
MspI	CCGG	1 cut(s)	810
MspR9I	CCNGG	1 cut(s)	1291
MvaI	CCWGG	1 cut(s)	1291
MvnI	CGCG	1 cut(s)	329
NcoI	CCATGG	1 cut(s)	174
NdeI	CATATG	1 cut(s)	712
NdeII	GATC	8 cut(s)	502, 544, 571, 593, 658, 670, 999, 1105
NlaIII	CATG	6 cut(s)	178, 497, 742, 890, 920, 1069
NlaIV	GGNNCC	4 cut(s)	519, 621, 762, 1001
NmuCI	GTSAC	4 cut(s)	166, 283, 469, 857
NsiI	ATGCAT	1 cut(s)	718
NspI	RCATGY	1 cut(s)	920
NspV	TTCGAA	1 cut(s)	1050
PaeR7I	CTCGAG	1 cut(s)	1143
PagI	TCATGA	1 cut(s)	886
PceI	AGGCCT	1 cut(s)	1085
PciI	ACATGT	1 cut(s)	916
PciSI	GCTCTTC	1 cut(s)	1174
PcsI	WCGNNNNNNNCGW	1 cut(s)	1047
PdmI	GAANNNNTTC	3 cut(s)	195, 965, 1256
PfeI	GAWTC	5 cut(s)	119, 245, 407, 643, 1026
PkrI	GCNGC	3 cut(s)	865, 878, 1235
PleI	GAGTC	2 cut(s)	7, 262
PpsI	GAGTC	2 cut(s)	7, 262
PscI	ACATGT	1 cut(s)	916
Psp124BI	GAGCTC	1 cut(s)	128
Psp6I	CCWGG	1 cut(s)	1289
PspFI	CCCAGC	2 cut(s)	625, 1111
PspGI	CCWGG	1 cut(s)	1289
PspN4I	GGNNCC	4 cut(s)	519, 621, 762, 1001
PspPI	GGNCC	3 cut(s)	517, 812, 980
PspXI	VCTCGAGB	1 cut(s)	1143
PstNI	CAGNNNCTG	1 cut(s)	785
PsuI	RGATCY	2 cut(s)	544, 999
RsaI	GTAC	2 cut(s)	365, 921
RsaNI	GTAC	2 cut(s)	364, 920
RseI	CAYNNNNRTG	1 cut(s)	885
SacI	GAGCTC	1 cut(s)	128
SapI	GCTCTTC	1 cut(s)	1174
SaqAI	TTAA	2 cut(s)	206, 987
SatI	GCNGC	3 cut(s)	864, 877, 1234
Sau3AI	GATC	8 cut(s)	502, 544, 571, 593, 658, 670, 999, 1105
Sau96I	GGNCC	3 cut(s)	517, 812, 980
SchI	GAGTC	2 cut(s)	7, 263
ScrFI	CCNGG	1 cut(s)	1291
SduI	GDGCHC	6 cut(s)	37, 128, 457, 624, 1078, 1298
SfaNI	GCATC	6 cut(s)	281, 450, 685, 725, 851, 928
SfcI	CTRYAG	1 cut(s)	852
Sfr274I	CTCGAG	1 cut(s)	1143
SfuI	TTCGAA	1 cut(s)	1050
SinI	GGWCC	3 cut(s)	517, 812, 980
SlaI	CTCGAG	1 cut(s)	1143
SmiMI	CAYNNNNRTG	1 cut(s)	885
SmlI	CTYRAG	4 cut(s)	956, 1057, 1079, 1143
SmoI	CTYRAG	4 cut(s)	956, 1057, 1079, 1143
Sse9I	AATT	9 cut(s)	195, 208, 323, 525, 720, 925, 1046, 1201, 1256
SseBI	AGGCCT	1 cut(s)	1085
SsiI	CCGC	4 cut(s)	1233, 1327, 1365, 1372
SspI	AATATT	1 cut(s)	68
SspMI	CTAG	1 cut(s)	344
SstI	GAGCTC	1 cut(s)	128
StuI	AGGCCT	1 cut(s)	1085
StyD4I	CCNGG	1 cut(s)	1289
StyI	CCWWGG	2 cut(s)	174, 1267
TaaI	ACNGT	4 cut(s)	166, 772, 781, 946
TaqI	TCGA	3 cut(s)	122, 1050, 1144
TasI	AATT	9 cut(s)	195, 208, 323, 525, 720, 925, 1046, 1201, 1256
TauI	GCSGC	1 cut(s)	1236
TfiI	GAWTC	5 cut(s)	119, 245, 407, 643, 1026
Tru1I	TTAA	2 cut(s)	206, 987
Tru9I	TTAA	2 cut(s)	206, 987
TscAI	CASTG	7 cut(s)	37, 171, 775, 799, 951, 1122, 1315
TseFI	GTSAC	4 cut(s)	166, 283, 469, 857
TseI	GCWGC	2 cut(s)	863, 876
Tsp45I	GTSAC	4 cut(s)	166, 283, 469, 857
TspDTI	ATGAA	2 cut(s)	482, 1269
TspGWI	ACGGA	2 cut(s)	743, 937
TspRI	CASTG	7 cut(s)	37, 171, 775, 799, 951, 1122, 1315
VneI	GTGCAC	1 cut(s)	1074
VpaK11BI	GGWCC	3 cut(s)	517, 812, 980
XagI	CCTNNNNNAGG	1 cut(s)	852
XapI	RAATTY	5 cut(s)	195, 323, 925, 1046, 1256
XceI	RCATGY	1 cut(s)	920
XhoI	CTCGAG	1 cut(s)	1143
XmnI	GAANNNNTTC	3 cut(s)	195, 965, 1256
XspI	CTAG	1 cut(s)	344
Zsp2I	ATGCAT	1 cut(s)	718

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.