MD14G1226700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011562
Physical Location & Seq
Reverse (-)
30803786 .. 30806232
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1226700.v1.1.491

Sequence Viewer

Length: 579 bp
ATGATGAGCAGGTGCGGTTACGAGCAGAGCGCCATGGTAGGCTGCGAGGGGATGGATTCAGTTGTGTGCCCAAAACCACGTCGATTGAGCTTCCTTACTTCTTCCTTGAACGACAACATCAGACCCTTCAGATACTTCAATCACAACCAATCGGAGATTGGAGATTCTGAAGCTGGGTCTGAGATTCTGGATATTATTCTCACCAAGGGGAATTGTGATGATAGATTCAGTACTAATCAATTGGCTTCATCGCCGCCGTTTTTCTGCGGGTCTCCGCCGAGCAGGGCATCAAATCCTGTAATCCAAGACGAGCATTTCGGCAACAGCAGCAATATGGCTCCGTTTTCGCCGACACAGCCCGTGTTGTCATCGCGCAACGGAGGCTGTGTCCGGATGAAATCCGGGGACAAGCCAGTCGCGCCCTCTGTTTTGGAAGAAGCAGAGTTTTGTGAGAAAAGTAGAGGGCTTTGTGCTTTTGGGAAAATATCAAGAGGAGGAGGAAGAAGATGTTTTTTTGAGGGGTCTTTGAGATGGAGAGAATGTAATTTTTGTATATACAAGTCATGTGAGTTGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.17

Weight (kDa)

7.44

Isoelectric Point (pI)

70.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 413
AccII CGCG 2 cut(s) 373, 419
AccIII TCCGGA 1 cut(s) 390
AciI CCGC 4 cut(s) 15, 254, 267, 275
AcuI CTGAAG 2 cut(s) 112, 189
AfaI GTAC 1 cut(s) 232
AgsI TTSAA 2 cut(s) 109, 139
AjiI CACGTC 1 cut(s) 80
AluBI AGCT 2 cut(s) 90, 173
AluI AGCT 2 cut(s) 90, 173
Alw26I GTCTC 1 cut(s) 276
Aor13HI TCCGGA 1 cut(s) 390
ApeKI GCWGC 2 cut(s) 42, 327
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AspLEI GCGC 3 cut(s) 32, 375, 421
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 1 cut(s) 193
BaeGI GKGCMC 1 cut(s) 71
BbvI GCAGC 2 cut(s) 29, 339
BccI CCATC 2 cut(s) 46, 525
BceAI ACGGC 1 cut(s) 241
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 276
BfoI RGCGCY 1 cut(s) 33
BisI GCNGC 3 cut(s) 43, 254, 328
BlsI GCNGC 3 cut(s) 44, 255, 329
BmcAI AGTACT 1 cut(s) 232
Bme1390I CCNGG 1 cut(s) 403
BmgBI CACGTC 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 403
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 3 cut(s) 33, 204, 402
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 4 cut(s) 372, 402, 526, 556
Bse1I ACTGG 1 cut(s) 413
BseAI TCCGGA 1 cut(s) 390
BseDI CCNNGG 3 cut(s) 33, 204, 402
BseGI GGATG 2 cut(s) 57, 399
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 2 cut(s) 507, 510
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 2 cut(s) 29, 339
BseYI CCCAGC 1 cut(s) 173
Bsh1236I CGCG 2 cut(s) 373, 419
BsiSI CCGG 2 cut(s) 391, 402
BslFI GGGAC 1 cut(s) 419
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 419
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 71
Bsp13I TCCGGA 1 cut(s) 390
Bsp143I GATC 1 cut(s) 573
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 4 cut(s) 15, 254, 267, 275
BspCNI CTCAG 1 cut(s) 172
BspEI TCCGGA 1 cut(s) 390
BspFNI CGCG 2 cut(s) 373, 419
BspLI GGNNCC 1 cut(s) 339
BspTNI GGTCTC 1 cut(s) 276
BsrI ACTGG 1 cut(s) 413
BssECI CCNNGG 3 cut(s) 33, 204, 402
BssMI GATC 1 cut(s) 573
BssT1I CCWWGG 2 cut(s) 33, 204
BstDEI CTNAG 1 cut(s) 180
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 2 cut(s) 57, 399
BstFNI CGCG 2 cut(s) 373, 419
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 3 cut(s) 32, 375, 421
BstKTI GATC 1 cut(s) 576
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 1 cut(s) 573
BstMWI GCNNNNNNNGC 4 cut(s) 327, 355, 381, 418
BstSCI CCNGG 1 cut(s) 401
BstSLI GKGCMC 1 cut(s) 71
BstUI CGCG 2 cut(s) 373, 419
BstV1I GCAGC 2 cut(s) 29, 339
BtgI CCRYGG 1 cut(s) 33
BtgZI GCGATG 2 cut(s) 234, 354
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 2 cut(s) 57, 399
CfoI GCGC 3 cut(s) 32, 375, 421
Csp6I GTAC 1 cut(s) 231
CspCI CAANNNNNGTGG 2 cut(s) 66, 101
CviAII CATG 2 cut(s) 34, 564
CviJI RGCY 9 cut(s) 42, 90, 173, 245, 338, 358, 384, 412, 466
CviKI_1 RGCY 9 cut(s) 42, 90, 173, 245, 338, 358, 384, 412, 466
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 1 cut(s) 180
DpnI GATC 1 cut(s) 575
DpnII GATC 1 cut(s) 573
DrdI GACNNNNNNGTC 1 cut(s) 413
DseDI GACNNNNNNGTC 1 cut(s) 413
EciI GGCGGA 1 cut(s) 264
Eco130I CCWWGG 2 cut(s) 33, 204
Eco31I GGTCTC 1 cut(s) 276
Eco57I CTGAAG 2 cut(s) 112, 189
EcoT14I CCWWGG 2 cut(s) 33, 204
ErhI CCWWGG 2 cut(s) 33, 204
FaeI CATG 2 cut(s) 37, 567
FaiI YATR 5 cut(s) 35, 335, 554, 556, 565
FaqI GGGAC 1 cut(s) 419
FatI CATG 2 cut(s) 33, 563
FauI CCCGC 1 cut(s) 260
Fnu4HI GCNGC 3 cut(s) 43, 254, 328
FokI GGATG 2 cut(s) 64, 406
Fsp4HI GCNGC 3 cut(s) 43, 254, 328
GlaI GCGC 3 cut(s) 31, 374, 420
GluI GCNGC 3 cut(s) 43, 254, 328
GsaI CCCAGC 1 cut(s) 177
HaeII RGCGCY 1 cut(s) 33
HapII CCGG 2 cut(s) 391, 402
HhaI GCGC 3 cut(s) 32, 375, 421
Hin1II CATG 2 cut(s) 37, 567
Hin6I GCGC 3 cut(s) 30, 373, 419
HinP1I GCGC 3 cut(s) 30, 373, 419
HinfI GANTC 4 cut(s) 56, 164, 184, 225
HpaII CCGG 2 cut(s) 391, 402
HphI GGTGA 1 cut(s) 193
Hpy188I TCNGA 5 cut(s) 122, 131, 154, 169, 181
Hpy188III TCNNGA 3 cut(s) 188, 391, 489
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 1 cut(s) 136
HpyCH4IV ACGT 1 cut(s) 79
HpyF10VI GCNNNNNNNGC 4 cut(s) 327, 355, 381, 418
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 2 cut(s) 37, 567
HspAI GCGC 3 cut(s) 30, 373, 419
Kpn2I TCCGGA 1 cut(s) 390
Kzo9I GATC 1 cut(s) 573
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 7 cut(s) 159, 173, 268, 309, 404, 415, 426
Lsp1109I GCAGC 2 cut(s) 29, 339
LweI GCATC 1 cut(s) 296
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 17
MalI GATC 1 cut(s) 575
MboI GATC 1 cut(s) 573
MboII GAAGA 4 cut(s) 93, 446, 513, 516
MfeI CAATTG 1 cut(s) 239
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 3 cut(s) 211, 239, 544
MnlI CCTC 8 cut(s) 40, 374, 433, 455, 485, 488, 491, 511
MroI TCCGGA 1 cut(s) 390
MspI CCGG 2 cut(s) 391, 402
MspR9I CCNGG 1 cut(s) 403
MunI CAATTG 1 cut(s) 239
MvnI CGCG 2 cut(s) 373, 419
MwoI GCNNNNNNNGC 4 cut(s) 327, 355, 381, 418
NciI CCSGG 1 cut(s) 403
NcoI CCATGG 1 cut(s) 33
NdeII GATC 1 cut(s) 573
NlaIII CATG 2 cut(s) 37, 567
NlaIV GGNNCC 1 cut(s) 339
NmeAIII GCCGAG 1 cut(s) 303
PfeI GAWTC 4 cut(s) 56, 164, 184, 225
PkrI GCNGC 3 cut(s) 44, 255, 329
PspFI CCCAGC 1 cut(s) 173
PspN4I GGNNCC 1 cut(s) 339
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
SatI GCNGC 3 cut(s) 43, 254, 328
Sau3AI GATC 1 cut(s) 573
ScaI AGTACT 1 cut(s) 232
ScrFI CCNGG 1 cut(s) 403
SduI GDGCHC 1 cut(s) 71
SetI ASST 4 cut(s) 14, 82, 92, 175
SfaNI GCATC 1 cut(s) 296
Sse9I AATT 3 cut(s) 211, 239, 544
SsiI CCGC 4 cut(s) 15, 254, 267, 275
StyD4I CCNGG 1 cut(s) 401
StyI CCWWGG 2 cut(s) 33, 204
TaiI ACGT 1 cut(s) 82
TaqI TCGA 1 cut(s) 82
TasI AATT 3 cut(s) 211, 239, 544
TatI WGTACW 1 cut(s) 230
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 4 cut(s) 56, 164, 184, 225
TseI GCWGC 2 cut(s) 42, 327
TspDTI ATGAA 2 cut(s) 237, 410
TspGWI ACGGA 2 cut(s) 330, 393
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
ZrmI AGTACT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.