pycom14g18810

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011562
Physical Location & Seq
Reverse (-)
20382252 .. 20384103
1852 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g18810.5

Sequence Viewer

Length: 753 bp
ATGGGAAATTTAAAAAAATATTTTTTCATTAAAATAAGTGTTAACAGAATCATCCTCTGTTTTCCCTCTCCGTCCCCTGTTTCTACGAATCATAAATACTCAGCCTCCCAACCCTTATCTCTCTCTCCCTCTCAGACGCCCAGAAAAAATCTCTGTTTTGCAGACCCGGTTTCTTCTTCCCCTCAAATCCTCCCGGAGGAGCCTCCTGTGGCTGCAGCTCAAGGCTTTATTGTTGGTGAGATCTCTCCAACAGAAGAAGCGAAGATGATGAGCAGGTGCGGTTACGAGCAGAGCGCCATGGTAGGCTGCGAGGGGATGGATTCAGTTGTGTGCCCAAAACCACGTCGTTTGAGCTTTCTAACTCCTTCCTTCAACGACAACGTCAGACCCTTTAGATACTTCAATCACAACCAATCGGAGATTGGAGATTCTGAAGCTGGGTCTGAGATTCTGGATATTATTCTCGCCAAGGGGAATTGTGATGATAGGTTTAGTACTAATCAATTGGCTTCGTCGCCGCCGTTTTTCTGCGGGTCTCCGCCGAGCAGGGCATCAAATCCTGTAATCCAAGACGAGCATTTCGGCAACAGCAGCAATATGGCTCCGTTTTTGCCGACACAGCCCATGTTGTCATCGCGCAACGGAGGCTGTGTCCGGATGAAATCCGGGGACAAGCCAGTCGCGGTGCGGATCGAAGGGTTTGATTGTCTTAGCAGGGACCGCAGGAACTGCAGCATCTCTGCTATAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

27.25

Weight (kDa)

8.03

Isoelectric Point (pI)

72.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 264
AasI GACNNNNNNGTC 1 cut(s) 677
Acc36I ACCTGC 1 cut(s) 264
AccII CGCG 2 cut(s) 637, 683
AccIII TCCGGA 1 cut(s) 654
AciI CCGC 7 cut(s) 279, 518, 531, 539, 683, 688, 721
AclWI GGATC 1 cut(s) 698
AcsI RAATTY 1 cut(s) 7
AcuI CTGAAG 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 1 cut(s) 496
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 2 cut(s) 373, 403
AjiI CACGTC 1 cut(s) 344
AluBI AGCT 4 cut(s) 218, 354, 437, 749
AluI AGCT 4 cut(s) 218, 354, 437, 749
Alw26I GTCTC 1 cut(s) 540
AlwI GGATC 1 cut(s) 698
AlwNI CAGNNNCTG 1 cut(s) 729
Aor13HI TCCGGA 1 cut(s) 654
ApeKI GCWGC 5 cut(s) 212, 215, 306, 591, 732
ApoI RAATTY 1 cut(s) 7
ArsI GACNNNNNNTTYG 2 cut(s) 563, 595
AspLEI GCGC 2 cut(s) 296, 639
AspS9I GGNCC 1 cut(s) 718
AsuC2I CCSGG 3 cut(s) 167, 194, 667
AsuHPI GGTGA 1 cut(s) 248
AvaII GGWCC 1 cut(s) 718
BaeGI GKGCMC 1 cut(s) 335
BbvI GCAGC 5 cut(s) 199, 227, 293, 603, 744
BccI CCATC 1 cut(s) 310
BceAI ACGGC 1 cut(s) 505
BcnI CCSGG 3 cut(s) 167, 194, 667
BcoDI GTCTC 1 cut(s) 540
BfmI CTRYAG 3 cut(s) 213, 730, 744
BfoI RGCGCY 1 cut(s) 297
BfuAI ACCTGC 1 cut(s) 264
BglII AGATCT 1 cut(s) 240
BisI GCNGC 6 cut(s) 213, 216, 307, 518, 592, 733
BlsI GCNGC 6 cut(s) 214, 217, 308, 519, 593, 734
BmcAI AGTACT 1 cut(s) 496
Bme1390I CCNGG 3 cut(s) 167, 194, 667
Bme18I GGWCC 1 cut(s) 718
BmgBI CACGTC 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 718
BmiI GGNNCC 3 cut(s) 201, 603, 719
BmrFI CCNGG 3 cut(s) 167, 194, 667
BmsI GCATC 2 cut(s) 560, 744
BpuEI CTTGAG 1 cut(s) 204
BpuMI CCSGG 3 cut(s) 167, 194, 667
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 540
BsaJI CCNNGG 3 cut(s) 297, 468, 666
BsaWI WCCGGW 1 cut(s) 654
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 636, 666
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 677
BseAI TCCGGA 1 cut(s) 654
BseDI CCNNGG 3 cut(s) 297, 468, 666
BseGI GGATG 3 cut(s) 51, 321, 663
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 3 cut(s) 114, 146, 435
BseNI ACTGG 1 cut(s) 677
BseRI GAGGAG 1 cut(s) 212
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 5 cut(s) 199, 227, 293, 603, 744
BseYI CCCAGC 1 cut(s) 437
Bsh1236I CGCG 2 cut(s) 637, 683
BsiSI CCGG 4 cut(s) 167, 194, 655, 666
BslFI GGGAC 3 cut(s) 58, 683, 731
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 540
BsmFI GGGAC 3 cut(s) 58, 683, 731
Bso31I GGTCTC 1 cut(s) 540
Bsp1286I GDGCHC 1 cut(s) 335
Bsp13I TCCGGA 1 cut(s) 654
Bsp143I GATC 2 cut(s) 240, 690
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 7 cut(s) 279, 518, 531, 539, 683, 688, 721
BspCNI CTCAG 3 cut(s) 113, 145, 436
BspEI TCCGGA 1 cut(s) 654
BspFNI CGCG 2 cut(s) 637, 683
BspLI GGNNCC 3 cut(s) 201, 603, 719
BspMAI CTGCAG 2 cut(s) 217, 734
BspMI ACCTGC 1 cut(s) 264
BspPI GGATC 1 cut(s) 698
BspTNI GGTCTC 1 cut(s) 540
BsrI ACTGG 1 cut(s) 677
BssECI CCNNGG 3 cut(s) 297, 468, 666
BssMI GATC 2 cut(s) 240, 690
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 2 cut(s) 297, 468
BstACI GRCGYC 1 cut(s) 137
BstAPI GCANNNNNTGC 1 cut(s) 729
BstDEI CTNAG 5 cut(s) 100, 132, 444, 710, 750
BstDSI CCRYGG 1 cut(s) 297
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 3 cut(s) 51, 321, 663
BstFNI CGCG 2 cut(s) 637, 683
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 2 cut(s) 296, 639
BstKTI GATC 2 cut(s) 243, 693
BstMAI GTCTC 1 cut(s) 540
BstMBI GATC 2 cut(s) 240, 690
BstMWI GCNNNNNNNGC 5 cut(s) 591, 619, 645, 720, 729
BstSCI CCNGG 3 cut(s) 165, 192, 665
BstSFI CTRYAG 3 cut(s) 213, 730, 744
BstSLI GKGCMC 1 cut(s) 335
BstUI CGCG 2 cut(s) 637, 683
BstV1I GCAGC 5 cut(s) 199, 227, 293, 603, 744
BstX2I RGATCY 1 cut(s) 240
BstYI RGATCY 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 297
BtgZI GCGATG 1 cut(s) 618
BtrI CACGTC 1 cut(s) 344
BtsCI GGATG 3 cut(s) 51, 321, 663
BveI ACCTGC 1 cut(s) 264
CaiI CAGNNNCTG 1 cut(s) 729
CfoI GCGC 2 cut(s) 296, 639
Cfr13I GGNCC 1 cut(s) 718
CseI GACGC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 495
CspCI CAANNNNNGTGG 2 cut(s) 330, 365
CviAII CATG 2 cut(s) 298, 625
CviQI GTAC 1 cut(s) 495
DdeI CTNAG 5 cut(s) 100, 132, 444, 710, 750
DpnI GATC 2 cut(s) 242, 692
DpnII GATC 2 cut(s) 240, 690
DraI TTTAAA 1 cut(s) 12
DrdI GACNNNNNNGTC 1 cut(s) 677
DseDI GACNNNNNNGTC 1 cut(s) 677
EciI GGCGGA 1 cut(s) 528
Eco130I CCWWGG 2 cut(s) 297, 468
Eco31I GGTCTC 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 718
Eco57I CTGAAG 1 cut(s) 453
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoT14I CCWWGG 2 cut(s) 297, 468
ErhI CCWWGG 2 cut(s) 297, 468
FaeI CATG 2 cut(s) 301, 628
FaiI YATR 5 cut(s) 93, 299, 599, 626, 746
FaqI GGGAC 3 cut(s) 58, 683, 731
FatI CATG 2 cut(s) 297, 624
FauI CCCGC 1 cut(s) 524
Fnu4HI GCNGC 6 cut(s) 213, 216, 307, 518, 592, 733
FokI GGATG 3 cut(s) 38, 328, 670
Fsp4HI GCNGC 6 cut(s) 213, 216, 307, 518, 592, 733
GlaI GCGC 2 cut(s) 295, 638
GluI GCNGC 6 cut(s) 213, 216, 307, 518, 592, 733
GsaI CCCAGC 1 cut(s) 441
HaeII RGCGCY 1 cut(s) 297
HapII CCGG 4 cut(s) 167, 194, 655, 666
HgaI GACGC 1 cut(s) 145
HhaI GCGC 2 cut(s) 296, 639
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 2 cut(s) 301, 628
Hin6I GCGC 2 cut(s) 294, 637
HinP1I GCGC 2 cut(s) 294, 637
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 5 cut(s) 48, 88, 320, 428, 448
HpaI GTTAAC 1 cut(s) 43
HpaII CCGG 4 cut(s) 167, 194, 655, 666
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 5 cut(s) 135, 386, 418, 433, 445
Hpy188III TCNNGA 2 cut(s) 452, 655
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 2 cut(s) 348, 517
HpyAV CCTTC 3 cut(s) 375, 379, 689
HpyCH4IV ACGT 2 cut(s) 343, 381
HpyCH4V TGCA 3 cut(s) 161, 215, 732
HpyF10VI GCNNNNNNNGC 5 cut(s) 591, 619, 645, 720, 729
HpyF3I CTNAG 5 cut(s) 100, 132, 444, 710, 750
HpySE526I ACGT 2 cut(s) 343, 381
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 2 cut(s) 301, 628
HspAI GCGC 2 cut(s) 294, 637
Kpn2I TCCGGA 1 cut(s) 654
KspAI GTTAAC 1 cut(s) 43
Kzo9I GATC 2 cut(s) 240, 690
LmnI GCTCC 2 cut(s) 199, 607
Lsp1109I GCAGC 5 cut(s) 199, 227, 293, 603, 744
LweI GCATC 2 cut(s) 560, 744
MaeII ACGT 2 cut(s) 343, 381
MaeIII GTNAC 1 cut(s) 281
MalI GATC 2 cut(s) 242, 692
MboI GATC 2 cut(s) 240, 690
MboII GAAGA 4 cut(s) 165, 168, 266, 274
MfeI CAATTG 1 cut(s) 503
MflI RGATCY 1 cut(s) 240
MhlI GDGCHC 1 cut(s) 335
MluCI AATT 3 cut(s) 7, 475, 503
MmeI TCCRAC 1 cut(s) 272
MroI TCCGGA 1 cut(s) 654
MseI TTAA 3 cut(s) 11, 30, 42
MspI CCGG 4 cut(s) 167, 194, 655, 666
MspR9I CCNGG 3 cut(s) 167, 194, 667
MunI CAATTG 1 cut(s) 503
MvnI CGCG 2 cut(s) 637, 683
MwoI GCNNNNNNNGC 5 cut(s) 591, 619, 645, 720, 729
NciI CCSGG 3 cut(s) 167, 194, 667
NcoI CCATGG 1 cut(s) 297
NdeII GATC 2 cut(s) 240, 690
NlaIII CATG 2 cut(s) 301, 628
NlaIV GGNNCC 3 cut(s) 201, 603, 719
NmeAIII GCCGAG 1 cut(s) 567
PaqCI CACCTGC 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 518
PfeI GAWTC 5 cut(s) 48, 88, 320, 428, 448
PflFI GACNNNGTC 1 cut(s) 380
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 6 cut(s) 214, 217, 308, 519, 593, 734
PspFI CCCAGC 1 cut(s) 437
PspN4I GGNNCC 3 cut(s) 201, 603, 719
PspPI GGNCC 1 cut(s) 718
PstI CTGCAG 2 cut(s) 217, 734
PstNI CAGNNNCTG 1 cut(s) 729
PsuI RGATCY 1 cut(s) 240
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
SaqAI TTAA 3 cut(s) 11, 30, 42
SatI GCNGC 6 cut(s) 213, 216, 307, 518, 592, 733
Sau3AI GATC 2 cut(s) 240, 690
Sau96I GGNCC 1 cut(s) 718
ScaI AGTACT 1 cut(s) 496
ScrFI CCNGG 3 cut(s) 167, 194, 667
SduI GDGCHC 1 cut(s) 335
SetI ASST 8 cut(s) 220, 278, 346, 356, 384, 439, 491, 751
SfaNI GCATC 2 cut(s) 560, 744
SfcI CTRYAG 3 cut(s) 213, 730, 744
SinI GGWCC 1 cut(s) 718
SmlI CTYRAG 1 cut(s) 219
SmoI CTYRAG 1 cut(s) 219
Sse9I AATT 3 cut(s) 7, 475, 503
SsiI CCGC 7 cut(s) 279, 518, 531, 539, 683, 688, 721
SspI AATATT 1 cut(s) 20
StyD4I CCNGG 3 cut(s) 165, 192, 665
StyI CCWWGG 2 cut(s) 297, 468
TaiI ACGT 2 cut(s) 346, 384
TaqI TCGA 1 cut(s) 693
TasI AATT 3 cut(s) 7, 475, 503
TatI WGTACW 1 cut(s) 494
TauI GCSGC 1 cut(s) 520
TfiI GAWTC 5 cut(s) 48, 88, 320, 428, 448
Tru1I TTAA 3 cut(s) 11, 30, 42
Tru9I TTAA 3 cut(s) 11, 30, 42
TseI GCWGC 5 cut(s) 212, 215, 306, 591, 732
TspDTI ATGAA 2 cut(s) 16, 674
TspGWI ACGGA 3 cut(s) 60, 594, 657
Tth111I GACNNNGTC 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 718
XagI CCTNNNNNAGG 1 cut(s) 194
XapI RAATTY 1 cut(s) 7
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
ZrmI AGTACT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.