pycom06g19270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011562
Physical Location & Seq
Reverse (-)
22729373 .. 22730471
1099 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19270.1

Sequence Viewer

Length: 489 bp
ATGATGAGCAGGTGCGGTTACGAGCAGAGCGCCACGGCAGGCTTCGAGGGAATGGATTCAGTTGTGTGCCCAAAACCACGTCGTTTCAGCCTTCTAAATCGTTCCTTCAACGACAACATCAGACCCTTCAGATACCTCAATCACAACCAATCGGAGATTGGAGATTCTGAAGCTGGGTCTGAGATTCTGGATATTATTCTCGCCAAGGGGAATTATGATGATAGATTCAGTAATAAGCAATTGGCTTCATCGCCGCCCTTTTTCTGCGGGTCTCCGCCGAGCAGGGCATCAAATCCCGTAATCCAAGACGAGCATTTCGGCAACAGCGGCAATATAGCTCCATTTTTGCCGGCACAGCCTATGTTGTCATCGCGCAACGGAGGCTGTGTCCGGATGAAGTCTGGGGACAAGCCAGTTGCAGTGCGGATCGAAGGGTTTGATTGTCTTAGCAGGGACCGCAGGAACTGCAGCATCTCTGCTGTAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

17.77

Weight (kDa)

8.26

Isoelectric Point (pI)

77.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 373
AccIII TCCGGA 1 cut(s) 390
AciI CCGC 7 cut(s) 15, 254, 267, 275, 327, 424, 457
AclWI GGATC 1 cut(s) 434
AcuI CTGAAG 2 cut(s) 112, 189
AgsI TTSAA 1 cut(s) 109
AjiI CACGTC 1 cut(s) 80
AluBI AGCT 3 cut(s) 173, 338, 485
AluI AGCT 3 cut(s) 173, 338, 485
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 1 cut(s) 434
AlwNI CAGNNNCTG 1 cut(s) 465
Aor13HI TCCGGA 1 cut(s) 390
ApeKI GCWGC 1 cut(s) 468
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 1 cut(s) 55
AspLEI GCGC 2 cut(s) 32, 375
AspS9I GGNCC 1 cut(s) 454
AvaII GGWCC 1 cut(s) 454
BaeGI GKGCMC 1 cut(s) 71
BbvI GCAGC 1 cut(s) 480
BceAI ACGGC 1 cut(s) 51
BcoDI GTCTC 1 cut(s) 276
BfmI CTRYAG 2 cut(s) 466, 480
BfoI RGCGCY 1 cut(s) 33
BisI GCNGC 3 cut(s) 254, 328, 469
BlsI GCNGC 3 cut(s) 255, 329, 470
Bme18I GGWCC 1 cut(s) 454
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 1 cut(s) 455
BmsI GCATC 2 cut(s) 296, 480
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 33, 204
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 1 cut(s) 413
BseAI TCCGGA 1 cut(s) 390
BseDI CCNNGG 2 cut(s) 33, 204
BseGI GGATG 1 cut(s) 399
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 413
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 1 cut(s) 480
BseYI CCCAGC 1 cut(s) 173
Bsh1236I CGCG 1 cut(s) 373
BsiSI CCGG 2 cut(s) 350, 391
BslFI GGGAC 2 cut(s) 419, 467
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 2 cut(s) 419, 467
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 71
Bsp13I TCCGGA 1 cut(s) 390
Bsp143I GATC 1 cut(s) 426
BspACI CCGC 7 cut(s) 15, 254, 267, 275, 327, 424, 457
BspCNI CTCAG 1 cut(s) 172
BspEI TCCGGA 1 cut(s) 390
BspFNI CGCG 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 455
BspMAI CTGCAG 1 cut(s) 470
BspPI GGATC 1 cut(s) 434
BspTNI GGTCTC 1 cut(s) 276
BsrFI RCCGGY 1 cut(s) 349
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 33, 204
BssMI GATC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 465
BstC8I GCNNGC 2 cut(s) 40, 351
BstDEI CTNAG 3 cut(s) 180, 446, 486
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 399
BstFNI CGCG 1 cut(s) 373
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 2 cut(s) 32, 375
BstKTI GATC 1 cut(s) 429
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 1 cut(s) 426
BstMWI GCNNNNNNNGC 5 cut(s) 327, 355, 381, 456, 465
BstSFI CTRYAG 2 cut(s) 466, 480
BstSLI GKGCMC 1 cut(s) 71
BstUI CGCG 1 cut(s) 373
BstV1I GCAGC 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 33
BtgZI GCGATG 2 cut(s) 234, 354
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 399
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 2 cut(s) 40, 351
CaiI CAGNNNCTG 1 cut(s) 465
CfoI GCGC 2 cut(s) 32, 375
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 454
CviJI RGCY 9 cut(s) 42, 90, 173, 245, 338, 358, 384, 412, 485
CviKI_1 RGCY 9 cut(s) 42, 90, 173, 245, 338, 358, 384, 412, 485
DdeI CTNAG 3 cut(s) 180, 446, 486
DpnI GATC 1 cut(s) 428
DpnII GATC 1 cut(s) 426
EciI GGCGGA 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 454
Eco57I CTGAAG 2 cut(s) 112, 189
EcoT14I CCWWGG 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 204
FaiI YATR 3 cut(s) 216, 335, 362
FaqI GGGAC 2 cut(s) 419, 467
FauI CCCGC 1 cut(s) 260
Fnu4HI GCNGC 3 cut(s) 254, 328, 469
FokI GGATG 1 cut(s) 406
Fsp4HI GCNGC 3 cut(s) 254, 328, 469
GlaI GCGC 2 cut(s) 31, 374
GluI GCNGC 3 cut(s) 254, 328, 469
GsaI CCCAGC 1 cut(s) 177
HaeII RGCGCY 1 cut(s) 33
HapII CCGG 2 cut(s) 350, 391
HhaI GCGC 2 cut(s) 32, 375
Hin6I GCGC 2 cut(s) 30, 373
HinP1I GCGC 2 cut(s) 30, 373
HinfI GANTC 4 cut(s) 56, 164, 184, 225
HpaII CCGG 2 cut(s) 350, 391
Hpy188I TCNGA 5 cut(s) 122, 131, 154, 169, 181
Hpy188III TCNNGA 2 cut(s) 188, 391
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 4 cut(s) 101, 115, 136, 425
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 2 cut(s) 419, 468
HpyF10VI GCNNNNNNNGC 5 cut(s) 327, 355, 381, 456, 465
HpyF3I CTNAG 3 cut(s) 180, 446, 486
HpySE526I ACGT 1 cut(s) 79
HspAI GCGC 2 cut(s) 30, 373
Kpn2I TCCGGA 1 cut(s) 390
KroI GCCGGC 1 cut(s) 349
KroNI GCCGGC 1 cut(s) 351
Kzo9I GATC 1 cut(s) 426
LmnI GCTCC 1 cut(s) 343
Lsp1109I GCAGC 1 cut(s) 480
LweI GCATC 2 cut(s) 296, 480
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 17
MalI GATC 1 cut(s) 428
MboI GATC 1 cut(s) 426
MfeI CAATTG 1 cut(s) 239
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 2 cut(s) 211, 239
MnlI CCTC 3 cut(s) 40, 146, 374
MroI TCCGGA 1 cut(s) 390
MroNI GCCGGC 1 cut(s) 349
MroXI GAANNNNTTC 1 cut(s) 55
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 2 cut(s) 350, 391
MunI CAATTG 1 cut(s) 239
MvnI CGCG 1 cut(s) 373
MwoI GCNNNNNNNGC 5 cut(s) 327, 355, 381, 456, 465
NaeI GCCGGC 1 cut(s) 351
NdeII GATC 1 cut(s) 426
NgoMIV GCCGGC 1 cut(s) 349
NlaIV GGNNCC 1 cut(s) 455
NmeAIII GCCGAG 1 cut(s) 303
PdiI GCCGGC 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 55
PfeI GAWTC 4 cut(s) 56, 164, 184, 225
PkrI GCNGC 3 cut(s) 255, 329, 470
PspFI CCCAGC 1 cut(s) 173
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 1 cut(s) 454
PstI CTGCAG 1 cut(s) 470
PstNI CAGNNNCTG 1 cut(s) 465
SatI GCNGC 3 cut(s) 254, 328, 469
Sau3AI GATC 1 cut(s) 426
Sau96I GGNCC 1 cut(s) 454
SduI GDGCHC 1 cut(s) 71
SetI ASST 6 cut(s) 14, 82, 138, 175, 340, 487
SfaNI GCATC 2 cut(s) 296, 480
SfcI CTRYAG 2 cut(s) 466, 480
SinI GGWCC 1 cut(s) 454
Sse9I AATT 2 cut(s) 211, 239
SsiI CCGC 7 cut(s) 15, 254, 267, 275, 327, 424, 457
StyI CCWWGG 1 cut(s) 204
TaiI ACGT 1 cut(s) 82
TaqI TCGA 2 cut(s) 45, 429
TasI AATT 2 cut(s) 211, 239
TauI GCSGC 2 cut(s) 256, 330
TfiI GAWTC 4 cut(s) 56, 164, 184, 225
TscAI CASTG 1 cut(s) 426
TseI GCWGC 1 cut(s) 468
TspDTI ATGAA 2 cut(s) 237, 410
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 454
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.