Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0010824

Physical Location & Seq

Genomic Coordinates Reverse (-)

30858344 .. 30859786

Gene Structure 1443 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1227400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 726 bp

ATGGAACTAGTAAACCAGTTTGCAGACCAATTCAATGTTCCAAGTGTCATGGAATATCCAAGCTTGAATTTCCACACCTACATGCCCTTCACAACCGACAGTTACTTGTTCAGCAATCAAGAACCCGAGTTCCCCGCCGGAAACATGGTCGAAAACTTTCCAAAATTAGAAGTTCATCAGACAGTTGCGGATGGAAGTGAAACCAAGAAGAGAATAGCAGTGGGTGATGCATCACAGAGCAGCTCTGGAATCTCTACTCCCCCAGTTTCTGAAACCGGGATTACGCGAAAAAATAGCGCAGGAAGAGGAAAGAGGGTCAAGAGCAATGAGAAGGAAGATGAGAAACCAAAGGAAGTTGTTCATGTTAGAGCCAGGAGAGGCCAAGCCACTGATAGCCACAGTCTAGCGGAAAGGGTTAGAAGAGGAAAAATCAATGAAAGACTGAAATGTTTGCAAGATATTGTCCCAGGGTGCTCTAACAAGACGATGGGAATGGCGGTAATGTTGGACGAGATAATTAACTATGTTCAGTCCTTGCAGAACCAGATTGAGTTCCTATCTATGAGGCTGGCTGCAGCAAGCTCTTTCTATGACTTCAACTCAAATACTGATGCTACGGAGACAATGCAGATAGCAGCAGAGTTGGAGAGATTGAAGAGAGAAGCTGCAGGATATGGAGGGTTTGGTGCTAGTTTCCAATCAACATCTTCAACTTGGTCCCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902446 GO:1902448 GO:1903506 GO:2000030 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

242

Amino Acids

26.74

Weight (kDa)

5.89

Isoelectric Point (pI)

48.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	132 - 180	2.6e-06	Helix-loop-helix DNA-binding domain
bHLH_SAC51	PF23173	137 - 182	5.9e-07	Transcription factor SAC51 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010824)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18400 AT1G18400 AT1G73830 AT1G73830
fragaria_vesca	FvH4_5g11950 FvH4_5g11950
malus_domestica	MD06G1217100.v1.1 MD14G1227400.v1.1
prunus_persica	Prupe.5G222200_v2.0.a1
pyrus_communis	pycom06g19340 pycom14g18890
rosa_chinensis	RchiOBHm_Chr7g0185551
rosa_laevigata	RLG00000004900
rosa_multiflora	Rmu_sc0010190.1_g000010
rosa_roxburghii	Rroxscaffold_3G00268820
rosa_samantha	Rh7AG072400 Rh7BG075400 Rh7CG075500 Rh7DG075200
rosa_wichuraiana	Rw7G006270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	286
AciI	CCGC	4 cut(s)	135, 188, 407, 497
AcsI	RAATTY	1 cut(s)	67
AgsI	TTSAA	5 cut(s)	34, 67, 598, 655, 711
AhlI	ACTAGT	1 cut(s)	7
AjnI	CCWGG	2 cut(s)	371, 466
AjuI	GAANNNNNNNTTGG	2 cut(s)	21, 53
AloI	GAACNNNNNNTCC	2 cut(s)	114, 146
AluBI	AGCT	4 cut(s)	63, 243, 582, 665
AluI	AGCT	4 cut(s)	63, 243, 582, 665
Alw21I	GWGCWC	1 cut(s)	476
Alw26I	GTCTC	1 cut(s)	614
AlwNI	CAGNNNCTG	1 cut(s)	269
Ama87I	CYCGRG	1 cut(s)	125
AoxI	GGCC	1 cut(s)	379
ApeKI	GCWGC	5 cut(s)	240, 572, 575, 635, 665
ApoI	RAATTY	1 cut(s)	67
Asp700I	GAANNNNTTC	2 cut(s)	156, 357
AspLEI	GCGC	1 cut(s)	299
AspS9I	GGNCC	1 cut(s)	717
AsuC2I	CCSGG	1 cut(s)	277
AsuHPI	GGTGA	1 cut(s)	236
AvaI	CYCGRG	1 cut(s)	125
AvaII	GGWCC	1 cut(s)	717
Bbv12I	GWGCWC	1 cut(s)	476
BbvI	GCAGC	5 cut(s)	252, 559, 587, 647, 652
BccI	CCATC	2 cut(s)	185, 481
BciT130I	CCWGG	2 cut(s)	373, 468
BcnI	CCSGG	1 cut(s)	277
BcoDI	GTCTC	1 cut(s)	614
BcuI	ACTAGT	1 cut(s)	7
BfaI	CTAG	3 cut(s)	8, 404, 690
BfmI	CTRYAG	2 cut(s)	573, 666
BisI	GCNGC	5 cut(s)	241, 573, 576, 636, 666
BlsI	GCNGC	5 cut(s)	242, 574, 577, 637, 667
Bme1390I	CCNGG	3 cut(s)	277, 373, 468
Bme18I	GGWCC	1 cut(s)	717
BmeT110I	CYCGRG	1 cut(s)	125
BmgT120I	GGNCC	1 cut(s)	717
BmiI	GGNNCC	1 cut(s)	719
BmrFI	CCNGG	3 cut(s)	277, 373, 468
BmrI	ACTGGG	1 cut(s)	257
BmsI	GCATC	3 cut(s)	217, 239, 601
BmuI	ACTGGG	1 cut(s)	257
BpuMI	CCSGG	1 cut(s)	277
BsaJI	CCNNGG	2 cut(s)	466, 467
Bse1I	ACTGG	2 cut(s)	16, 263
Bse3DI	GCAATG	1 cut(s)	331
BseBI	CCWGG	2 cut(s)	373, 468
BseDI	CCNNGG	2 cut(s)	466, 467
BseGI	GGATG	1 cut(s)	196
BseMI	GCAATG	1 cut(s)	331
BseNI	ACTGG	2 cut(s)	16, 263
BseXI	GCAGC	5 cut(s)	252, 559, 587, 647, 652
Bsh1236I	CGCG	1 cut(s)	286
BshFI	GGCC	1 cut(s)	381
BsiHKAI	GWGCWC	1 cut(s)	476
BsiHKCI	CYCGRG	1 cut(s)	125
BsiSI	CCGG	2 cut(s)	138, 276
BslFI	GGGAC	2 cut(s)	449, 703
BsmAI	GTCTC	1 cut(s)	614
BsmFI	GGGAC	2 cut(s)	449, 703
BsnI	GGCC	1 cut(s)	381
BsoBI	CYCGRG	1 cut(s)	125
Bsp1286I	GDGCHC	1 cut(s)	476
BspACI	CCGC	4 cut(s)	135, 188, 407, 497
BspANI	GGCC	1 cut(s)	381
BspFNI	CGCG	1 cut(s)	286
BspLI	GGNNCC	1 cut(s)	719
BspMAI	CTGCAG	2 cut(s)	577, 670
BsrDI	GCAATG	1 cut(s)	331
BsrI	ACTGG	2 cut(s)	16, 263
BssECI	CCNNGG	2 cut(s)	466, 467
Bst2UI	CCWGG	2 cut(s)	373, 468
Bst4CI	ACNGT	3 cut(s)	101, 184, 401
Bst6I	CTCTTC	4 cut(s)	203, 298, 415, 650
BstC8I	GCNNGC	2 cut(s)	570, 580
BstF5I	GGATG	1 cut(s)	196
BstFNI	CGCG	1 cut(s)	286
BstHHI	GCGC	1 cut(s)	299
BstMAI	GTCTC	1 cut(s)	614
BstNI	CCWGG	2 cut(s)	373, 468
BstNSI	RCATGY	1 cut(s)	85
BstSCI	CCNGG	3 cut(s)	275, 371, 466
BstSFI	CTRYAG	2 cut(s)	573, 666
BstUI	CGCG	1 cut(s)	286
BstV1I	GCAGC	5 cut(s)	252, 559, 587, 647, 652
BsuRI	GGCC	1 cut(s)	381
BtsCI	GGATG	1 cut(s)	196
BtsI	GCAGTG	1 cut(s)	225
BtsIMutI	CAGTG	2 cut(s)	225, 387
Cac8I	GCNNGC	2 cut(s)	570, 580
CaiI	CAGNNNCTG	1 cut(s)	269
CfoI	GCGC	1 cut(s)	299
Cfr13I	GGNCC	1 cut(s)	717
CviAII	CATG	4 cut(s)	49, 82, 145, 362
Eam1104I	CTCTTC	4 cut(s)	203, 298, 415, 650
EarI	CTCTTC	4 cut(s)	203, 298, 415, 650
Eco47I	GGWCC	1 cut(s)	717
Eco88I	CYCGRG	1 cut(s)	125
EcoRII	CCWGG	2 cut(s)	371, 466
EcoT22I	ATGCAT	1 cut(s)	232
FaeI	CATG	4 cut(s)	52, 85, 148, 365
FaiI	YATR	8 cut(s)	50, 83, 146, 363, 525, 563, 591, 675
FaqI	GGGAC	2 cut(s)	449, 703
FatI	CATG	4 cut(s)	48, 81, 144, 361
FauI	CCCGC	1 cut(s)	142
Fnu4HI	GCNGC	5 cut(s)	241, 573, 576, 636, 666
FokI	GGATG	1 cut(s)	203
Fsp4HI	GCNGC	5 cut(s)	241, 573, 576, 636, 666
FspBI	CTAG	3 cut(s)	8, 404, 690
GlaI	GCGC	1 cut(s)	298
GluI	GCNGC	5 cut(s)	241, 573, 576, 636, 666
HaeIII	GGCC	1 cut(s)	381
HapII	CCGG	2 cut(s)	138, 276
HhaI	GCGC	1 cut(s)	299
Hin1II	CATG	4 cut(s)	52, 85, 148, 365
Hin6I	GCGC	1 cut(s)	297
HinP1I	GCGC	1 cut(s)	297
HindIII	AAGCTT	1 cut(s)	61
HinfI	GANTC	1 cut(s)	249
HpaII	CCGG	2 cut(s)	138, 276
HphI	GGTGA	1 cut(s)	236
Hpy166II	GTNNAC	1 cut(s)	13
Hpy188I	TCNGA	2 cut(s)	180, 271
Hpy188III	TCNNGA	3 cut(s)	119, 246, 319
Hpy8I	GTNNAC	1 cut(s)	13
HpyAV	CCTTC	2 cut(s)	97, 325
HpyCH4III	ACNGT	3 cut(s)	101, 184, 401
HpyCH4V	TGCA	7 cut(s)	23, 230, 454, 538, 575, 628, 668
Hsp92II	CATG	4 cut(s)	52, 85, 148, 365
HspAI	GCGC	1 cut(s)	297
Lsp1109I	GCAGC	5 cut(s)	252, 559, 587, 647, 652
LweI	GCATC	3 cut(s)	217, 239, 601
MaeI	CTAG	3 cut(s)	8, 404, 690
MaeIII	GTNAC	1 cut(s)	101
MboII	GAAGA	6 cut(s)	220, 315, 347, 432, 667, 699
MhlI	GDGCHC	1 cut(s)	476
MluCI	AATT	4 cut(s)	29, 67, 164, 516
MmeI	TCCRAC	2 cut(s)	486, 624
MnlI	CCTC	6 cut(s)	299, 306, 371, 416, 558, 671
Mph1103I	ATGCAT	1 cut(s)	232
MroXI	GAANNNNTTC	2 cut(s)	156, 357
MseI	TTAA	1 cut(s)	519
MslI	CAYNNNNRTG	1 cut(s)	80
MspI	CCGG	2 cut(s)	138, 276
MspR9I	CCNGG	3 cut(s)	277, 373, 468
MvaI	CCWGG	2 cut(s)	373, 468
MvnI	CGCG	1 cut(s)	286
NciI	CCSGG	1 cut(s)	277
NlaIII	CATG	4 cut(s)	52, 85, 148, 365
NlaIV	GGNNCC	1 cut(s)	719
NsiI	ATGCAT	1 cut(s)	232
NspI	RCATGY	1 cut(s)	85
PasI	CCCWGGG	1 cut(s)	467
PdmI	GAANNNNTTC	2 cut(s)	156, 357
PfeI	GAWTC	1 cut(s)	249
PkrI	GCNGC	5 cut(s)	242, 574, 577, 637, 667
Psp6I	CCWGG	2 cut(s)	371, 466
PspGI	CCWGG	2 cut(s)	371, 466
PspN4I	GGNNCC	1 cut(s)	719
PspPI	GGNCC	1 cut(s)	717
PstI	CTGCAG	2 cut(s)	577, 670
PstNI	CAGNNNCTG	1 cut(s)	269
RseI	CAYNNNNRTG	1 cut(s)	80
SaqAI	TTAA	1 cut(s)	519
SatI	GCNGC	5 cut(s)	241, 573, 576, 636, 666
Sau96I	GGNCC	1 cut(s)	717
ScrFI	CCNGG	3 cut(s)	277, 373, 468
SduI	GDGCHC	1 cut(s)	476
SetI	ASST	5 cut(s)	65, 80, 245, 584, 667
SfaNI	GCATC	3 cut(s)	217, 239, 601
SfcI	CTRYAG	2 cut(s)	573, 666
SinI	GGWCC	1 cut(s)	717
SmiMI	CAYNNNNRTG	1 cut(s)	80
SpeI	ACTAGT	1 cut(s)	7
Sse9I	AATT	4 cut(s)	29, 67, 164, 516
SsiI	CCGC	4 cut(s)	135, 188, 407, 497
SspMI	CTAG	3 cut(s)	8, 404, 690
StyD4I	CCNGG	3 cut(s)	275, 371, 466
TaaI	ACNGT	3 cut(s)	101, 184, 401
TaqI	TCGA	1 cut(s)	150
TasI	AATT	4 cut(s)	29, 67, 164, 516
TfiI	GAWTC	1 cut(s)	249
Tru1I	TTAA	1 cut(s)	519
Tru9I	TTAA	1 cut(s)	519
TscAI	CASTG	2 cut(s)	225, 394
TseI	GCWGC	5 cut(s)	240, 572, 575, 635, 665
TspDTI	ATGAA	3 cut(s)	164, 350, 450
TspGWI	ACGGA	1 cut(s)	632
TspRI	CASTG	2 cut(s)	225, 394
VpaK11BI	GGWCC	1 cut(s)	717
XapI	RAATTY	1 cut(s)	67
XceI	RCATGY	1 cut(s)	85
XmnI	GAANNNNTTC	2 cut(s)	156, 357
XspI	CTAG	3 cut(s)	8, 404, 690
Zsp2I	ATGCAT	1 cut(s)	232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.