MD14G1238600.v1.1

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011991
Physical Location & Seq
Reverse (-)
31777371 .. 31779365
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1238600.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGGTCTCATTCTCTCAAAAAATATTGTGAATTTGATTTGCAATTCTTCGAATTCTCCCGTTTTCTCCAAGAAAAATGGCGGAGATTTCATCCCCATGCAAATTGAGAACATGGTTTCTTTGGTTTCCCGGACCGGAAGACAATTCCAGCGCTATGAAAAAGGTTGCCGCCAAGTTGTAGGATGCATACCATACAGATATCGGAAAACCGATGAGGAATTGGAAGTTCTTGTGATTAGTTCACAGAAGGGGAAAGGAATGTTGTTCCCAAAGGGAGGTTGGGAAATGGATGAGTCAATTGAAGAGGCGGCGAAAAGAGAGACGTTTGAGGAAGCTGGTGTGACTGGCCATCTTGAAACTAGATTGGGATCGTGGCGTTACACGAGCAAAACCCAAGGCACGGTTCATGAGGGCTACATGTTCCCTTTGCTTGTTCAGCAGCAGTTGGAACTTTGGCCCGAGAAAAGTTCCCGAAAACGTGAATGGGTGACGGTCTCTGAAGCTAGAGAAGCATGTCAAAATTGGTGGATGAAGGAGGCTTTGGAGAAATTAGTTTGTCGACAGCCCCAACCGAAAGGAGAGGAAGGAGAAGCAACTTGTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

202

Amino Acids

23.03

Weight (kDa)

7.56

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 70 - 183 9.2e-15 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011991)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18300 AT1G73540
fragaria_vesca FvH4_5g17620 FvH4_5g17620
malus_domestica MD06G1232000.v1.1 MD14G1238600.v1.1
prunus_persica Prupe.5G235900_v2.0.a1
pyrus_communis pycom06g20590 pycom14g19970
rosa_chinensis RchiOBHm_Chr7g0178041
rosa_laevigata RLG00000005479
rosa_multiflora Rmu_sc0000425.1_g000002
rosa_roxburghii Rroxscaffold_3G00275250
rosa_rugosa Rorug06G0412500 Rorug06G0412500
rosa_samantha Rh7AG012100 Rh7CG012100 Rh7DG012400
rosa_wichuraiana Rw7G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 559
AciI CCGC 3 cut(s) 81, 169, 308
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 2 cut(s) 31, 52
AcuI CTGAAG 1 cut(s) 519
AfaI GTAC 1 cut(s) 601
AfeI AGCGCT 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 275, 400
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 2 cut(s) 302, 356
AjuI GAANNNNNNNTTGG 4 cut(s) 387, 419, 524, 556
AluBI AGCT 2 cut(s) 335, 503
AluI AGCT 2 cut(s) 335, 503
Alw26I GTCTC 3 cut(s) 11, 314, 499
AlwI GGATC 1 cut(s) 376
Ama87I CYCGRG 1 cut(s) 458
Aor51HI AGCGCT 1 cut(s) 152
AoxI GGCC 2 cut(s) 346, 455
ApeKI GCWGC 1 cut(s) 439
ApoI RAATTY 2 cut(s) 31, 52
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 2 cut(s) 132, 456
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 499
AsuII TTCGAA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 1 cut(s) 132
BalI TGGCCA 1 cut(s) 348
BauI CACGAG 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 145
BbvI GCAGC 1 cut(s) 451
BccI CCATC 1 cut(s) 357
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 3 cut(s) 11, 314, 499
BfaI CTAG 2 cut(s) 360, 504
BfoI RGCGCY 1 cut(s) 154
BisI GCNGC 3 cut(s) 169, 309, 440
BlsI GCNGC 3 cut(s) 170, 310, 441
Bme1390I CCNGG 1 cut(s) 130
Bme18I GGWCC 1 cut(s) 132
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 132, 456
BmrFI CCNGG 1 cut(s) 130
BmsI GCATC 1 cut(s) 173
BpiI GAAGAC 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 130
BsaAI YACGTR 1 cut(s) 603
BsaBI GATNNNNATC 1 cut(s) 367
BsaI GGTCTC 2 cut(s) 11, 499
BsaJI CCNNGG 1 cut(s) 394
BsaWI WCCGGW 1 cut(s) 134
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 400
Bse1I ACTGG 1 cut(s) 349
Bse8I GATNNNNATC 1 cut(s) 367
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 4 cut(s) 90, 188, 295, 534
BseJI GATNNNNATC 1 cut(s) 367
BseLI CCNNNNNNNGG 2 cut(s) 275, 400
BseNI ACTGG 1 cut(s) 349
BseXI GCAGC 1 cut(s) 451
BshFI GGCC 2 cut(s) 348, 457
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 2 cut(s) 130, 135
BslI CCNNNNNNNGG 2 cut(s) 275, 400
BsmAI GTCTC 3 cut(s) 11, 314, 499
BsmBI CGTCTC 1 cut(s) 314
BsnI GGCC 2 cut(s) 348, 457
Bso31I GGTCTC 2 cut(s) 11, 499
BsoBI CYCGRG 1 cut(s) 458
Bsp119I TTCGAA 1 cut(s) 50
Bsp143I GATC 1 cut(s) 368
BspACI CCGC 3 cut(s) 81, 169, 308
BspANI GGCC 2 cut(s) 348, 457
BspHI TCATGA 1 cut(s) 406
BspPI GGATC 1 cut(s) 376
BspT104I TTCGAA 1 cut(s) 50
BspTNI GGTCTC 2 cut(s) 11, 499
BsrI ACTGG 1 cut(s) 349
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 1 cut(s) 368
BssSI CACGAG 1 cut(s) 382
BssT1I CCWWGG 1 cut(s) 394
Bst2BI CACGAG 1 cut(s) 382
Bst4CI ACNGT 2 cut(s) 403, 493
Bst6I CTCTTC 1 cut(s) 297
BstBAI YACGTR 1 cut(s) 603
BstBI TTCGAA 1 cut(s) 50
BstF5I GGATG 4 cut(s) 90, 188, 295, 534
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 1 cut(s) 371
BstMAI GTCTC 3 cut(s) 11, 314, 499
BstMBI GATC 1 cut(s) 368
BstMWI GCNNNNNNNGC 2 cut(s) 436, 509
BstNSI RCATGY 2 cut(s) 421, 516
BstSCI CCNGG 1 cut(s) 128
BstSNI TACGTA 1 cut(s) 603
BstV1I GCAGC 1 cut(s) 451
BstV2I GAAGAC 1 cut(s) 145
BsuRI GGCC 2 cut(s) 348, 457
BtsCI GGATG 4 cut(s) 90, 188, 295, 534
CciI TCATGA 1 cut(s) 406
CfoI GCGC 1 cut(s) 153
Cfr13I GGNCC 2 cut(s) 132, 456
CpoI CGGWCCG 1 cut(s) 132
Csp6I GTAC 1 cut(s) 600
CspCI CAANNNNNGTGG 2 cut(s) 506, 541
CspI CGGWCCG 1 cut(s) 132
CviAII CATG 5 cut(s) 97, 112, 407, 418, 513
CviJI RGCY 7 cut(s) 335, 348, 414, 457, 503, 539, 565
CviKI_1 RGCY 7 cut(s) 335, 348, 414, 457, 503, 539, 565
CviQI GTAC 1 cut(s) 600
DpnI GATC 1 cut(s) 370
DpnII GATC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
EciI GGCGGA 1 cut(s) 96
Eco105I TACGTA 1 cut(s) 603
Eco130I CCWWGG 1 cut(s) 394
Eco31I GGTCTC 2 cut(s) 11, 499
Eco32I GATATC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 132
Eco47III AGCGCT 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 519
Eco88I CYCGRG 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 52
EcoRV GATATC 1 cut(s) 200
EcoT14I CCWWGG 1 cut(s) 394
EcoT22I ATGCAT 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 394
Esp3I CGTCTC 1 cut(s) 314
FaeI CATG 5 cut(s) 100, 115, 410, 421, 516
FaiI YATR 8 cut(s) 98, 113, 156, 188, 193, 408, 419, 514
FatI CATG 5 cut(s) 96, 111, 406, 417, 512
FblI GTMKAC 1 cut(s) 559
Fnu4HI GCNGC 3 cut(s) 169, 309, 440
FokI GGATG 4 cut(s) 77, 195, 302, 541
Fsp4HI GCNGC 3 cut(s) 169, 309, 440
FspBI CTAG 2 cut(s) 360, 504
GlaI GCGC 1 cut(s) 152
GluI GCNGC 3 cut(s) 169, 309, 440
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 2 cut(s) 348, 457
HapII CCGG 2 cut(s) 130, 135
HhaI GCGC 1 cut(s) 153
Hin1II CATG 5 cut(s) 100, 115, 410, 421, 516
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HincII GTYRAC 1 cut(s) 560
HindII GTYRAC 1 cut(s) 560
HinfI GANTC 1 cut(s) 293
HpaII CCGG 2 cut(s) 130, 135
HphI GGTGA 1 cut(s) 499
Hpy166II GTNNAC 2 cut(s) 242, 560
Hpy188I TCNGA 2 cut(s) 204, 499
Hpy188III TCNNGA 3 cut(s) 353, 407, 471
Hpy8I GTNNAC 2 cut(s) 242, 560
HpyAV CCTTC 3 cut(s) 241, 526, 578
HpyCH4III ACNGT 2 cut(s) 403, 493
HpyCH4IV ACGT 3 cut(s) 323, 478, 602
HpyCH4V TGCA 3 cut(s) 42, 100, 186
HpyF10VI GCNNNNNNNGC 2 cut(s) 436, 509
HpySE526I ACGT 3 cut(s) 323, 478, 602
Hsp92II CATG 5 cut(s) 100, 115, 410, 421, 516
HspAI GCGC 1 cut(s) 151
Kzo9I GATC 1 cut(s) 368
LpnPI CCDG 5 cut(s) 143, 148, 161, 321, 330
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 1 cut(s) 173
MaeI CTAG 2 cut(s) 360, 504
MaeII ACGT 3 cut(s) 323, 478, 602
MaeIII GTNAC 3 cut(s) 340, 377, 487
MalI GATC 1 cut(s) 370
MboI GATC 1 cut(s) 368
MboII GAAGA 3 cut(s) 39, 150, 314
MfeI CAATTG 1 cut(s) 297
MlsI TGGCCA 1 cut(s) 348
MluCI AATT 9 cut(s) 31, 43, 52, 102, 143, 218, 297, 520, 548
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 7 cut(s) 208, 269, 298, 322, 403, 529, 574
Mox20I TGGCCA 1 cut(s) 348
Mph1103I ATGCAT 1 cut(s) 188
MscI TGGCCA 1 cut(s) 348
MslI CAYNNNNRTG 1 cut(s) 95
Msp20I TGGCCA 1 cut(s) 348
MspI CCGG 2 cut(s) 130, 135
MspR9I CCNGG 1 cut(s) 130
MunI CAATTG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 436, 509
NciI CCSGG 1 cut(s) 130
NdeII GATC 1 cut(s) 368
NlaIII CATG 5 cut(s) 100, 115, 410, 421, 516
NmuCI GTSAC 2 cut(s) 340, 487
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 2 cut(s) 421, 516
NspV TTCGAA 1 cut(s) 50
PagI TCATGA 1 cut(s) 406
PciI ACATGT 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 128
PkrI GCNGC 3 cut(s) 170, 310, 441
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 603
PscI ACATGT 1 cut(s) 417
PspPI GGNCC 2 cut(s) 132, 456
RsaI GTAC 1 cut(s) 601
RsaNI GTAC 1 cut(s) 600
RseI CAYNNNNRTG 1 cut(s) 95
Rsr2I CGGWCCG 1 cut(s) 132
RsrII CGGWCCG 1 cut(s) 132
SalI GTCGAC 1 cut(s) 558
SatI GCNGC 3 cut(s) 169, 309, 440
Sau3AI GATC 1 cut(s) 368
Sau96I GGNCC 2 cut(s) 132, 456
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 1 cut(s) 130
SetI ASST 7 cut(s) 166, 280, 326, 337, 481, 505, 605
SfaNI GCATC 1 cut(s) 173
SfuI TTCGAA 1 cut(s) 50
SinI GGWCC 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 95
SnaBI TACGTA 1 cut(s) 603
Sse9I AATT 9 cut(s) 31, 43, 52, 102, 143, 218, 297, 520, 548
SsiI CCGC 3 cut(s) 81, 169, 308
SspI AATATT 1 cut(s) 25
SspMI CTAG 2 cut(s) 360, 504
StyD4I CCNGG 1 cut(s) 128
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 2 cut(s) 403, 493
TaiI ACGT 3 cut(s) 326, 481, 605
TaqI TCGA 2 cut(s) 50, 559
TasI AATT 9 cut(s) 31, 43, 52, 102, 143, 218, 297, 520, 548
TauI GCSGC 2 cut(s) 171, 311
TseFI GTSAC 2 cut(s) 340, 487
TseI GCWGC 1 cut(s) 439
Tsp45I GTSAC 2 cut(s) 340, 487
TspDTI ATGAA 4 cut(s) 79, 171, 395, 545
VpaK11BI GGWCC 1 cut(s) 132
XapI RAATTY 2 cut(s) 31, 52
XceI RCATGY 2 cut(s) 421, 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 559
XspI CTAG 2 cut(s) 360, 504
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.