RLG00000005479

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011991
Physical Location & Seq
Reverse (-)
67971178 .. 67972799
1622 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005479

Sequence Viewer

Length: 858 bp
ATGGGCATCTCTCTATCCAGAAATATAGCAAGTTTCATTTTCTCCTCTGAGTCCTCTCCCCTCTTCTCCAAGAAAAATGGTGGTATTCCCATTCCCATGCAATTGGAGAACATGGTCTCCTTGGTTTCCCGGACCGGAAGGCATTTGCAGCGCTACGATAAAGGCTGCCGCCAAGTTGTAGGATGCATACCCTACAGATATAGGCAAACAAAACAAGAATCTTTAACTACGGAGGAAATAGAAGTTCTTGTGGTTAGTTCACAAAAGGGTCAAGGAATGTTGTTCCCAAAGGGAGGTTGGGAACTTGATGAGTCTATTGAAGAAGCGGCTTACAGAGAAACGCTAGAGGAGGCTGGTGTGACTGGCCATCTTGAGCCTAAATTGGGCAAATGGCGTTACAAGAGCAAAAGCCAGGGCACTTTTCATGAGGGGTACATGTTCCCTTTGCTTGTCAACGAGCAGTTCGAGTCTTGGCCTGAGAAGACTGTCAGGAAACGAGAATGGATGACTCTTGCTGAAGCTAAAGAAGCATGCCAAAATTGGTGGATGAAGGAAGCTTTGGATGAATTTGTTCGTCGACAAACGCAGCACCAAAAGAAAGAAGAACAAGATGAGCGTGTTTTTCTGTCGCTGTACAATGTAGTTGAAGATCTAGAAACAGAACGCCTGTTAGATGTTTATTTGGCTCCTGTGTCTTTCATTGGAAGGGCTTGGCTTAGTTCAAAATACAGGTTCTGGACCACAGAGACCGAGTGTGGCATAAAGCTTGGTATATCTCAGAATGTGGGTGGATATGCTTCATTTGAAGTGGAAGATGGGATTGCTTTTGTCATCATGAAAACCACTAAAGCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

286

Amino Acids

32.87

Weight (kDa)

5.79

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 79 - 189 8.5e-14 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011991)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18300 AT1G73540
fragaria_vesca FvH4_5g17620 FvH4_5g17620
malus_domestica MD06G1232000.v1.1 MD14G1238600.v1.1
prunus_persica Prupe.5G235900_v2.0.a1
pyrus_communis pycom06g20590 pycom14g19970
rosa_chinensis RchiOBHm_Chr7g0178041
rosa_laevigata RLG00000005479
rosa_multiflora Rmu_sc0000425.1_g000002
rosa_roxburghii Rroxscaffold_3G00275250
rosa_rugosa Rorug06G0412500 Rorug06G0412500
rosa_samantha Rh7AG012100 Rh7CG012100 Rh7DG012400
rosa_wichuraiana Rw7G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 577
AciI CCGC 2 cut(s) 169, 326
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 1 cut(s) 566
AcuI CTGAAG 1 cut(s) 537
AfaI GTAC 2 cut(s) 434, 635
AfeI AGCGCT 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 293, 383
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 4 cut(s) 320, 647, 723, 806
AjnI CCWGG 1 cut(s) 411
AjuI GAANNNNNNNTTGG 2 cut(s) 542, 574
AloI GAACNNNNNNTCC 2 cut(s) 101, 133
AluBI AGCT 3 cut(s) 521, 557, 766
AluI AGCT 3 cut(s) 521, 557, 766
Alw26I GTCTC 2 cut(s) 121, 740
AlwNI CAGNNNCTG 1 cut(s) 735
Aor51HI AGCGCT 1 cut(s) 152
AoxI GGCC 2 cut(s) 364, 473
ApeKI GCWGC 3 cut(s) 148, 165, 586
ApoI RAATTY 1 cut(s) 566
Asp700I GAANNNNTTC 1 cut(s) 570
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 2 cut(s) 132, 738
AsuC2I CCSGG 1 cut(s) 130
AvaII GGWCC 2 cut(s) 132, 738
BaeGI GKGCMC 1 cut(s) 419
BalI TGGCCA 1 cut(s) 366
BbsI GAAGAC 1 cut(s) 488
BbvI GCAGC 3 cut(s) 152, 160, 598
BccI CCATC 2 cut(s) 375, 809
BciT130I CCWGG 1 cut(s) 413
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 2 cut(s) 121, 740
BfaI CTAG 2 cut(s) 344, 653
BfmI CTRYAG 1 cut(s) 193
BfoI RGCGCY 1 cut(s) 154
BglII AGATCT 1 cut(s) 649
BisI GCNGC 5 cut(s) 149, 166, 169, 327, 587
BlsI GCNGC 5 cut(s) 150, 167, 170, 328, 588
Bme1390I CCNGG 2 cut(s) 130, 413
Bme18I GGWCC 2 cut(s) 132, 738
BmgT120I GGNCC 2 cut(s) 132, 738
BmiI GGNNCC 1 cut(s) 687
BmrFI CCNGG 2 cut(s) 130, 413
BmsI GCATC 2 cut(s) 15, 173
BpiI GAAGAC 1 cut(s) 488
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 130
BsaI GGTCTC 2 cut(s) 121, 740
BsaJI CCNNGG 2 cut(s) 120, 412
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 383
Bse1I ACTGG 1 cut(s) 367
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 2 cut(s) 120, 412
BseGI GGATG 4 cut(s) 188, 510, 552, 568
BseLI CCNNNNNNNGG 2 cut(s) 293, 383
BseMII CTCAG 3 cut(s) 39, 468, 791
BseNI ACTGG 1 cut(s) 367
BseRI GAGGAG 2 cut(s) 34, 362
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 3 cut(s) 152, 160, 598
BshFI GGCC 2 cut(s) 366, 475
BsiSI CCGG 2 cut(s) 130, 135
BslI CCNNNNNNNGG 2 cut(s) 293, 383
BsmAI GTCTC 2 cut(s) 121, 740
BsnI GGCC 2 cut(s) 366, 475
Bso31I GGTCTC 2 cut(s) 121, 740
Bsp1286I GDGCHC 1 cut(s) 419
Bsp1407I TGTACA 1 cut(s) 633
Bsp143I GATC 1 cut(s) 649
BspACI CCGC 2 cut(s) 169, 326
BspANI GGCC 2 cut(s) 366, 475
BspCNI CTCAG 3 cut(s) 40, 469, 790
BspHI TCATGA 2 cut(s) 424, 834
BspLI GGNNCC 1 cut(s) 687
BspTNI GGTCTC 2 cut(s) 121, 740
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 120, 412
BssMI GATC 1 cut(s) 649
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 413
Bst4CI ACNGT 1 cut(s) 487
Bst6I CTCTTC 1 cut(s) 68
BstAUI TGTACA 1 cut(s) 633
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 4 cut(s) 48, 477, 716, 777
BstF5I GGATG 4 cut(s) 188, 510, 552, 568
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 1 cut(s) 652
BstMAI GTCTC 2 cut(s) 121, 740
BstMBI GATC 1 cut(s) 649
BstMWI GCNNNNNNNGC 2 cut(s) 148, 527
BstNI CCWGG 1 cut(s) 413
BstNSI RCATGY 2 cut(s) 439, 534
BstSCI CCNGG 2 cut(s) 128, 411
BstSFI CTRYAG 1 cut(s) 193
BstSLI GKGCMC 1 cut(s) 419
BstV1I GCAGC 3 cut(s) 152, 160, 598
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 649
BstXI CCANNNNNNTGG 1 cut(s) 103
BstYI RGATCY 1 cut(s) 649
BsuRI GGCC 2 cut(s) 366, 475
BtsCI GGATG 4 cut(s) 188, 510, 552, 568
Cac8I GCNNGC 1 cut(s) 532
CaiI CAGNNNCTG 1 cut(s) 735
CciI TCATGA 2 cut(s) 424, 834
CfoI GCGC 1 cut(s) 153
Cfr13I GGNCC 2 cut(s) 132, 738
CpoI CGGWCCG 1 cut(s) 132
Csp6I GTAC 2 cut(s) 433, 634
CspCI CAANNNNNGTGG 2 cut(s) 524, 559
CspI CGGWCCG 1 cut(s) 132
CviAII CATG 6 cut(s) 97, 112, 425, 436, 531, 835
CviQI GTAC 2 cut(s) 433, 634
DdeI CTNAG 4 cut(s) 48, 477, 716, 777
DpnI GATC 1 cut(s) 651
DpnII GATC 1 cut(s) 649
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 120
Eco31I GGTCTC 2 cut(s) 121, 740
Eco47I GGWCC 2 cut(s) 132, 738
Eco47III AGCGCT 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 537
EcoRII CCWGG 1 cut(s) 411
EcoT14I CCWWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 6 cut(s) 100, 115, 428, 439, 534, 838
FatI CATG 6 cut(s) 96, 111, 424, 435, 530, 834
FblI GTMKAC 1 cut(s) 577
Fnu4HI GCNGC 5 cut(s) 149, 166, 169, 327, 587
FokI GGATG 4 cut(s) 195, 517, 559, 575
Fsp4HI GCNGC 5 cut(s) 149, 166, 169, 327, 587
FspBI CTAG 2 cut(s) 344, 653
GlaI GCGC 1 cut(s) 152
GluI GCNGC 5 cut(s) 149, 166, 169, 327, 587
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 2 cut(s) 366, 475
HapII CCGG 2 cut(s) 130, 135
HhaI GCGC 1 cut(s) 153
Hin1II CATG 6 cut(s) 100, 115, 428, 439, 534, 838
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HincII GTYRAC 2 cut(s) 454, 578
HindII GTYRAC 2 cut(s) 454, 578
HindIII AAGCTT 2 cut(s) 555, 764
HinfI GANTC 5 cut(s) 50, 218, 311, 467, 508
HpaII CCGG 2 cut(s) 130, 135
Hpy166II GTNNAC 3 cut(s) 260, 454, 578
Hpy188I TCNGA 2 cut(s) 49, 780
Hpy188III TCNNGA 7 cut(s) 18, 371, 425, 490, 653, 736, 835
Hpy8I GTNNAC 3 cut(s) 260, 454, 578
Hpy99I CGWCG 1 cut(s) 579
HpyAV CCTTC 3 cut(s) 132, 544, 699
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4V TGCA 3 cut(s) 100, 148, 186
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 527
HpyF3I CTNAG 4 cut(s) 48, 477, 716, 777
Hsp92II CATG 6 cut(s) 100, 115, 428, 439, 534, 838
HspAI GCGC 1 cut(s) 151
Kzo9I GATC 1 cut(s) 649
LmnI GCTCC 1 cut(s) 691
Lsp1109I GCAGC 3 cut(s) 152, 160, 598
LweI GCATC 2 cut(s) 15, 173
MaeI CTAG 2 cut(s) 344, 653
MaeIII GTNAC 2 cut(s) 358, 395
MalI GATC 1 cut(s) 651
MboI GATC 1 cut(s) 649
MboII GAAGA 6 cut(s) 55, 332, 493, 614, 659, 824
MfeI CAATTG 1 cut(s) 101
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 1 cut(s) 419
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 4 cut(s) 101, 380, 538, 566
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 4 cut(s) 59, 320, 476, 502
MnlI CCTC 8 cut(s) 55, 64, 71, 226, 287, 340, 343, 421
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 570
MscI TGGCCA 1 cut(s) 366
MseI TTAA 2 cut(s) 224, 856
MslI CAYNNNNRTG 1 cut(s) 95
Msp20I TGGCCA 1 cut(s) 366
MspI CCGG 2 cut(s) 130, 135
MspR9I CCNGG 2 cut(s) 130, 413
MunI CAATTG 1 cut(s) 101
MvaI CCWGG 1 cut(s) 413
MwoI GCNNNNNNNGC 2 cut(s) 148, 527
NciI CCSGG 1 cut(s) 130
NdeII GATC 1 cut(s) 649
NlaIII CATG 6 cut(s) 100, 115, 428, 439, 534, 838
NlaIV GGNNCC 1 cut(s) 687
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 2 cut(s) 439, 534
PaeI GCATGC 1 cut(s) 534
PagI TCATGA 2 cut(s) 424, 834
PciI ACATGT 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 1 cut(s) 218
PfoI TCCNGGA 1 cut(s) 128
PkrI GCNGC 5 cut(s) 150, 167, 170, 328, 588
PleI GAGTC 4 cut(s) 58, 319, 475, 502
PpsI GAGTC 4 cut(s) 58, 319, 475, 502
PscI ACATGT 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 411
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 1 cut(s) 687
PspPI GGNCC 2 cut(s) 132, 738
PstNI CAGNNNCTG 1 cut(s) 735
PsuI RGATCY 1 cut(s) 649
RsaI GTAC 2 cut(s) 434, 635
RsaNI GTAC 2 cut(s) 433, 634
RseI CAYNNNNRTG 1 cut(s) 95
Rsr2I CGGWCCG 1 cut(s) 132
RsrII CGGWCCG 1 cut(s) 132
SalI GTCGAC 1 cut(s) 576
SaqAI TTAA 2 cut(s) 224, 856
SatI GCNGC 5 cut(s) 149, 166, 169, 327, 587
Sau3AI GATC 1 cut(s) 649
Sau96I GGNCC 2 cut(s) 132, 738
SchI GAGTC 4 cut(s) 59, 320, 476, 502
ScrFI CCNGG 2 cut(s) 130, 413
SduI GDGCHC 1 cut(s) 419
SetI ASST 5 cut(s) 298, 523, 559, 734, 768
SfaNI GCATC 2 cut(s) 15, 173
SfcI CTRYAG 1 cut(s) 193
SinI GGWCC 2 cut(s) 132, 738
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SphI GCATGC 1 cut(s) 534
Sse9I AATT 4 cut(s) 101, 380, 538, 566
SsiI CCGC 2 cut(s) 169, 326
SspMI CTAG 2 cut(s) 344, 653
StyD4I CCNGG 2 cut(s) 128, 411
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 1 cut(s) 487
TaqI TCGA 2 cut(s) 465, 577
TaqII GACCGA 1 cut(s) 764
TasI AATT 4 cut(s) 101, 380, 538, 566
TatI WGTACW 1 cut(s) 633
TauI GCSGC 2 cut(s) 171, 329
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 2 cut(s) 224, 856
Tru9I TTAA 2 cut(s) 224, 856
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 3 cut(s) 148, 165, 586
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 7 cut(s) 25, 413, 563, 579, 688, 789, 851
TspGWI ACGGA 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 132, 738
XapI RAATTY 1 cut(s) 566
XbaI TCTAGA 1 cut(s) 652
XceI RCATGY 2 cut(s) 439, 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 577
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 2 cut(s) 344, 653
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.